Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43697.t1 | XP_003615484.1 | 98 | 147 | 3 | 0 | 1 | 147 | 1 | 147 | 1.10E-81 | 312.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43697.t1 | G7K658 | 98.0 | 147 | 3 | 0 | 1 | 147 | 1 | 147 | 8.2e-82 | 312.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05410 | MS.gene43697 | 0.806419 | 8.30E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43697.t1 | MTR_5g068660 | 97.959 | 147 | 3 | 0 | 1 | 147 | 1 | 147 | 1.11e-109 | 308 |
| MS.gene43697.t1 | MTR_3g093320 | 65.986 | 147 | 22 | 3 | 1 | 147 | 1 | 119 | 1.82e-59 | 180 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43697.t1 | AT5G24610 | 71.918 | 146 | 38 | 2 | 1 | 145 | 1 | 144 | 8.15e-72 | 212 |
| MS.gene43697.t1 | AT3G49550 | 69.128 | 149 | 42 | 3 | 1 | 147 | 1 | 147 | 1.49e-70 | 209 |
Find 42 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTATCTGTCAAGTATTT+TGG | 0.086537 | 5.1:+53294619 | MS.gene43697:CDS |
| TTCCAGTCGCATTTGTGAAC+AGG | 0.332197 | 5.1:-53294659 | None:intergenic |
| TTCCCCAAACACTTTCGTTT+TGG | 0.336557 | 5.1:-53294685 | None:intergenic |
| CCTGTTCTTCAGTTGGAGTA+AGG | 0.348208 | 5.1:-53294823 | None:intergenic |
| ATGCCAAAACGAAAGTGTTT+GGG | 0.350359 | 5.1:+53294682 | MS.gene43697:CDS |
| AATGCCAAAACGAAAGTGTT+TGG | 0.356504 | 5.1:+53294681 | MS.gene43697:CDS |
| GAAGTTTCAATGGAGGTATA+TGG | 0.356919 | 5.1:+53294566 | None:intergenic |
| CTCACTGCCTGTTCTTCAGT+TGG | 0.398254 | 5.1:-53294830 | None:intergenic |
| GGAGGTATATGGAAAATCTA+TGG | 0.409354 | 5.1:+53294577 | MS.gene43697:CDS |
| GACAGATAAATAACATTTGA+AGG | 0.421919 | 5.1:-53294608 | None:intergenic |
| CCTTACTCCAACTGAAGAAC+AGG | 0.443337 | 5.1:+53294823 | MS.gene43697:CDS |
| ATGTTTCGCTGCAAGCTGAC+AGG | 0.449969 | 5.1:+53294710 | MS.gene43697:CDS |
| CTGTTCTTCAGTTGGAGTAA+GGG | 0.465524 | 5.1:-53294822 | None:intergenic |
| GCAGAGAAAGATCTCTCAAA+AGG | 0.477866 | 5.1:-53294947 | None:intergenic |
| TATCCATGCCATCTCCAACT+TGG | 0.481067 | 5.1:-53294988 | None:intergenic |
| TCTCCAACTTGGCTGCAGAT+AGG | 0.485639 | 5.1:-53294977 | None:intergenic |
| GGAAAATTTGACGACTTGCT+AGG | 0.493282 | 5.1:-53294799 | None:intergenic |
| TTGACGACTTGCTAGGCAAA+GGG | 0.507539 | 5.1:-53294792 | None:intergenic |
| GGTTATCATAGAGAATTCTC+TGG | 0.526901 | 5.1:-53294763 | None:intergenic |
| CGGTGGTTGTAAGCGTAGGC+GGG | 0.527174 | 5.1:+53294904 | MS.gene43697:CDS |
| CCCCTGTTCACAAATGCGAC+TGG | 0.528911 | 5.1:+53294657 | MS.gene43697:CDS |
| TCGCATTTGTGAACAGGGGT+TGG | 0.530520 | 5.1:-53294653 | None:intergenic |
| AGTCCTATCTGCAGCCAAGT+TGG | 0.539791 | 5.1:+53294974 | MS.gene43697:CDS |
| TTGCTAGGCAAAGGGAGCTA+TGG | 0.541413 | 5.1:-53294784 | None:intergenic |
| TCCAGTCGCATTTGTGAACA+GGG | 0.542299 | 5.1:-53294658 | None:intergenic |
| TGTTCTTCAGTTGGAGTAAG+GGG | 0.550641 | 5.1:-53294821 | None:intergenic |
| ATCTGCAGCCAAGTTGGAGA+TGG | 0.552457 | 5.1:+53294980 | MS.gene43697:CDS |
| CAGCCAAGTTGGAGATGGCA+TGG | 0.553002 | 5.1:+53294985 | MS.gene43697:CDS |
| TGTTTCGCTGCAAGCTGACA+GGG | 0.556183 | 5.1:+53294711 | MS.gene43697:CDS |
| ATCTCTCAAAAGGAGAAGGA+TGG | 0.556591 | 5.1:-53294937 | None:intergenic |
| ACGGTGGTTGTAAGCGTAGG+CGG | 0.561151 | 5.1:+53294903 | MS.gene43697:CDS |
| AGGCAGTGAGAGGCGTTCGA+AGG | 0.561321 | 5.1:+53294843 | MS.gene43697:CDS |
| AAAGATCTCTCAAAAGGAGA+AGG | 0.563086 | 5.1:-53294941 | None:intergenic |
| GAACAGGGGTTGGACCATCA+TGG | 0.588831 | 5.1:-53294643 | None:intergenic |
| TTTGACGACTTGCTAGGCAA+AGG | 0.589756 | 5.1:-53294793 | None:intergenic |
| AATTCACACGTTGATAATAA+CGG | 0.592198 | 5.1:+53294884 | MS.gene43697:CDS |
| ATAACGGTGGTTGTAAGCGT+AGG | 0.611122 | 5.1:+53294900 | MS.gene43697:CDS |
| TGCCAAAACGAAAGTGTTTG+GGG | 0.620413 | 5.1:+53294683 | MS.gene43697:CDS |
| ACTGAAGAACAGGCAGTGAG+AGG | 0.628808 | 5.1:+53294833 | MS.gene43697:CDS |
| GTTCTTCAGTTGGAGTAAGG+GGG | 0.636393 | 5.1:-53294820 | None:intergenic |
| CCAGTCGCATTTGTGAACAG+GGG | 0.641312 | 5.1:-53294657 | None:intergenic |
| TCACACGTTGATAATAACGG+TGG | 0.701112 | 5.1:+53294887 | MS.gene43697:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATCTGTCAAGTATTTT+GGG | + | chr5.1:53294620-53294639 | MS.gene43697:CDS | 20.0% |
| !!! | TATTTATCTGTCAAGTATTT+TGG | + | chr5.1:53294619-53294638 | MS.gene43697:CDS | 20.0% |
| ! | GACAGATAAATAACATTTGA+AGG | - | chr5.1:53294611-53294630 | None:intergenic | 25.0% |
| !!! | AATTCACACGTTGATAATAA+CGG | + | chr5.1:53294884-53294903 | MS.gene43697:CDS | 25.0% |
| AAAGATCTCTCAAAAGGAGA+AGG | - | chr5.1:53294944-53294963 | None:intergenic | 35.0% | |
| GGTTATCATAGAGAATTCTC+TGG | - | chr5.1:53294766-53294785 | None:intergenic | 35.0% | |
| ! | GGAGGTATATGGAAAATCTA+TGG | + | chr5.1:53294577-53294596 | MS.gene43697:CDS | 35.0% |
| !! | AATGCCAAAACGAAAGTGTT+TGG | + | chr5.1:53294681-53294700 | MS.gene43697:CDS | 35.0% |
| !! | ATGCCAAAACGAAAGTGTTT+GGG | + | chr5.1:53294682-53294701 | MS.gene43697:CDS | 35.0% |
| ATCTCTCAAAAGGAGAAGGA+TGG | - | chr5.1:53294940-53294959 | None:intergenic | 40.0% | |
| CTGTTCTTCAGTTGGAGTAA+GGG | - | chr5.1:53294825-53294844 | None:intergenic | 40.0% | |
| GCAGAGAAAGATCTCTCAAA+AGG | - | chr5.1:53294950-53294969 | None:intergenic | 40.0% | |
| TGTTCTTCAGTTGGAGTAAG+GGG | - | chr5.1:53294824-53294843 | None:intergenic | 40.0% | |
| ! | GGAAAATTTGACGACTTGCT+AGG | - | chr5.1:53294802-53294821 | None:intergenic | 40.0% |
| ! | TTCCCCAAACACTTTCGTTT+TGG | - | chr5.1:53294688-53294707 | None:intergenic | 40.0% |
| !! | TCAAGTATTTTGGGCCATGA+TGG | + | chr5.1:53294629-53294648 | MS.gene43697:CDS | 40.0% |
| !! | TCACACGTTGATAATAACGG+TGG | + | chr5.1:53294887-53294906 | MS.gene43697:CDS | 40.0% |
| !! | TGCCAAAACGAAAGTGTTTG+GGG | + | chr5.1:53294683-53294702 | MS.gene43697:CDS | 40.0% |
| ATAACGGTGGTTGTAAGCGT+AGG | + | chr5.1:53294900-53294919 | MS.gene43697:CDS | 45.0% | |
| CCTGTTCTTCAGTTGGAGTA+AGG | - | chr5.1:53294826-53294845 | None:intergenic | 45.0% | |
| CCTTACTCCAACTGAAGAAC+AGG | + | chr5.1:53294823-53294842 | MS.gene43697:CDS | 45.0% | |
| GTTCTTCAGTTGGAGTAAGG+GGG | - | chr5.1:53294823-53294842 | None:intergenic | 45.0% | |
| TATCCATGCCATCTCCAACT+TGG | - | chr5.1:53294991-53295010 | None:intergenic | 45.0% | |
| TCCAGTCGCATTTGTGAACA+GGG | - | chr5.1:53294661-53294680 | None:intergenic | 45.0% | |
| TTCCAGTCGCATTTGTGAAC+AGG | - | chr5.1:53294662-53294681 | None:intergenic | 45.0% | |
| !! | TTGACGACTTGCTAGGCAAA+GGG | - | chr5.1:53294795-53294814 | None:intergenic | 45.0% |
| !! | TTTGACGACTTGCTAGGCAA+AGG | - | chr5.1:53294796-53294815 | None:intergenic | 45.0% |
| ACTGAAGAACAGGCAGTGAG+AGG | + | chr5.1:53294833-53294852 | MS.gene43697:CDS | 50.0% | |
| AGTCCTATCTGCAGCCAAGT+TGG | + | chr5.1:53294974-53294993 | MS.gene43697:CDS | 50.0% | |
| ATGTTTCGCTGCAAGCTGAC+AGG | + | chr5.1:53294710-53294729 | MS.gene43697:CDS | 50.0% | |
| CCAGTCGCATTTGTGAACAG+GGG | - | chr5.1:53294660-53294679 | None:intergenic | 50.0% | |
| CTCACTGCCTGTTCTTCAGT+TGG | - | chr5.1:53294833-53294852 | None:intergenic | 50.0% | |
| TCGCATTTGTGAACAGGGGT+TGG | - | chr5.1:53294656-53294675 | None:intergenic | 50.0% | |
| TCTCCAACTTGGCTGCAGAT+AGG | - | chr5.1:53294980-53294999 | None:intergenic | 50.0% | |
| TGTTTCGCTGCAAGCTGACA+GGG | + | chr5.1:53294711-53294730 | MS.gene43697:CDS | 50.0% | |
| TTGCTAGGCAAAGGGAGCTA+TGG | - | chr5.1:53294787-53294806 | None:intergenic | 50.0% | |
| ! | ATCTGCAGCCAAGTTGGAGA+TGG | + | chr5.1:53294980-53294999 | MS.gene43697:CDS | 50.0% |
| ACGGTGGTTGTAAGCGTAGG+CGG | + | chr5.1:53294903-53294922 | MS.gene43697:CDS | 55.0% | |
| CCCCTGTTCACAAATGCGAC+TGG | + | chr5.1:53294657-53294676 | MS.gene43697:CDS | 55.0% | |
| GAACAGGGGTTGGACCATCA+TGG | - | chr5.1:53294646-53294665 | None:intergenic | 55.0% | |
| ! | CAGCCAAGTTGGAGATGGCA+TGG | + | chr5.1:53294985-53295004 | MS.gene43697:CDS | 55.0% |
| CGGTGGTTGTAAGCGTAGGC+GGG | + | chr5.1:53294904-53294923 | MS.gene43697:CDS | 60.0% | |
| !! | AGGCAGTGAGAGGCGTTCGA+AGG | + | chr5.1:53294843-53294862 | MS.gene43697:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 53294575 | 53295018 | 53294575 | ID=MS.gene43697 |
| chr5.1 | mRNA | 53294575 | 53295018 | 53294575 | ID=MS.gene43697.t1;Parent=MS.gene43697 |
| chr5.1 | exon | 53294575 | 53295018 | 53294575 | ID=MS.gene43697.t1.exon1;Parent=MS.gene43697.t1 |
| chr5.1 | CDS | 53294575 | 53295018 | 53294575 | ID=cds.MS.gene43697.t1;Parent=MS.gene43697.t1 |
| Gene Sequence |
| Protein sequence |
