Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43730.t1 | XP_003615537.2 | 74.8 | 876 | 207 | 5 | 1 | 870 | 1 | 868 | 0.00E+00 | 1198.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43730.t1 | A4FVR1 | 31.1 | 280 | 124 | 11 | 619 | 870 | 337 | 575 | 1.9e-12 | 76.3 |
| MS.gene43730.t1 | A4FVR1 | 35.3 | 153 | 82 | 4 | 3 | 150 | 6 | 146 | 2.5e-12 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43730.t1 | G7KE04 | 74.8 | 876 | 207 | 5 | 1 | 870 | 1 | 868 | 0.0e+00 | 1198.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052965 | MS.gene43730 | 0.815312 | 9.86E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43730.t1 | MTR_5g069290 | 83.333 | 876 | 132 | 5 | 1 | 870 | 1 | 868 | 0.0 | 1404 |
| MS.gene43730.t1 | MTR_5g096290 | 48.347 | 242 | 106 | 6 | 635 | 870 | 545 | 773 | 5.19e-50 | 189 |
| MS.gene43730.t1 | MTR_5g096290 | 35.503 | 169 | 98 | 4 | 397 | 562 | 371 | 531 | 2.44e-13 | 74.7 |
| MS.gene43730.t1 | MTR_8g019650 | 26.743 | 703 | 367 | 34 | 225 | 870 | 395 | 1006 | 1.36e-33 | 140 |
| MS.gene43730.t1 | MTR_8g019650 | 56.250 | 96 | 40 | 2 | 14 | 108 | 22 | 116 | 6.39e-22 | 102 |
| MS.gene43730.t1 | MTR_8g088190 | 52.055 | 73 | 32 | 1 | 10 | 82 | 18 | 87 | 6.87e-17 | 86.3 |
| MS.gene43730.t1 | MTR_3g048270 | 40.449 | 89 | 47 | 3 | 10 | 98 | 23 | 105 | 7.80e-12 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43730.t1 | AT3G07660 | 46.102 | 885 | 418 | 22 | 1 | 870 | 1 | 841 | 0.0 | 623 |
| MS.gene43730.t1 | AT3G13990 | 28.770 | 504 | 290 | 20 | 10 | 474 | 15 | 488 | 7.92e-27 | 118 |
| MS.gene43730.t1 | AT3G13990 | 28.911 | 505 | 288 | 21 | 10 | 474 | 15 | 488 | 8.36e-27 | 118 |
| MS.gene43730.t1 | AT3G13222 | 49.153 | 118 | 46 | 4 | 5 | 121 | 9 | 113 | 3.41e-23 | 105 |
| MS.gene43730.t1 | AT1G55820 | 46.218 | 119 | 50 | 5 | 3 | 120 | 6 | 111 | 1.95e-19 | 93.6 |
| MS.gene43730.t1 | AT4G18150 | 47.727 | 88 | 41 | 3 | 10 | 97 | 15 | 97 | 7.25e-16 | 82.8 |
| MS.gene43730.t1 | AT5G46380 | 33.117 | 154 | 88 | 6 | 9 | 149 | 65 | 216 | 5.94e-12 | 70.1 |
| MS.gene43730.t1 | AT5G46380 | 33.117 | 154 | 88 | 6 | 9 | 149 | 10 | 161 | 6.35e-12 | 69.7 |
| MS.gene43730.t1 | AT1G29350 | 47.191 | 89 | 45 | 2 | 10 | 98 | 17 | 103 | 1.47e-11 | 68.9 |
Find 250 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTGATCTCTTTGATATTC+TGG | 0.088348 | 5.1:-53732010 | None:intergenic |
| GATGAGTGGTAGTGGGTTTA+AGG | 0.186674 | 5.1:+53731956 | None:intergenic |
| TAAATTCACAGGTACTTTAT+TGG | 0.208556 | 5.1:-53738118 | None:intergenic |
| GCAACAGTTGCTTCTGAAAC+TGG | 0.237265 | 5.1:+53735790 | MS.gene43730:CDS |
| GGTAGTTTGGGAGTCAATTT+TGG | 0.247910 | 5.1:+53737096 | MS.gene43730:CDS |
| GGAAGACAGGGGCAGGTTGC+TGG | 0.253745 | 5.1:-53740151 | None:intergenic |
| TGTGGGACCATTGGCAATAA+TGG | 0.263583 | 5.1:-53735626 | None:intergenic |
| TGCAACCTCATTTGATTTAT+CGG | 0.269819 | 5.1:-53735770 | None:intergenic |
| CACTCAGAAGCTTCTTCTTC+AGG | 0.272953 | 5.1:+53732097 | MS.gene43730:CDS |
| AATACATGTGGTCAAAATTC+TGG | 0.285084 | 5.1:+53736871 | MS.gene43730:CDS |
| AAATTCACAGGTACTTTATT+GGG | 0.291071 | 5.1:-53738117 | None:intergenic |
| AGTCCTTCTCTTTCGTCTGC+TGG | 0.293341 | 5.1:+53735679 | MS.gene43730:CDS |
| CGGGTCATCTAGTGCTATTC+AGG | 0.293663 | 5.1:+53738158 | MS.gene43730:CDS |
| ATTCCTGGGGCTGACACATT+TGG | 0.295584 | 5.1:-53740332 | None:intergenic |
| ACCGGCAGTTAATTTGTTCT+TGG | 0.306812 | 5.1:-53735968 | None:intergenic |
| CATGTAATGCCAAATGCTTT+TGG | 0.314911 | 5.1:+53740410 | MS.gene43730:CDS |
| TCTTGAGGAGATTGAGCATT+TGG | 0.318940 | 5.1:-53738601 | None:intergenic |
| GGAAGAAATACTGCTGTTAC+AGG | 0.321232 | 5.1:+53735122 | MS.gene43730:CDS |
| GAGATAACGGTTCAATCTTC+TGG | 0.330603 | 5.1:+53735709 | MS.gene43730:CDS |
| ACAAAACCATAGCTGTAATT+TGG | 0.334471 | 5.1:-53738252 | None:intergenic |
| TTCCGTGGGCAGCCTGAGTC+GGG | 0.336024 | 5.1:-53741184 | None:intergenic |
| CCTCCAAACTACATTCCTTA+TGG | 0.336677 | 5.1:+53740203 | MS.gene43730:CDS |
| GGAGCTGAGAACCCAACATT+GGG | 0.344599 | 5.1:-53738278 | None:intergenic |
| CTCAGCATGCACAGATGAAT+TGG | 0.346650 | 5.1:+53741318 | MS.gene43730:CDS |
| CAGCACCTGGTCGGTAAAGC+TGG | 0.351254 | 5.1:-53738498 | None:intergenic |
| AATTTGTTCTTCTCAGAATC+AGG | 0.352162 | 5.1:-53737066 | None:intergenic |
| TCAGGTACTATGATATGATT+TGG | 0.352194 | 5.1:-53737048 | None:intergenic |
| GCAGCTGTCACTGCTTGTGC+GGG | 0.362104 | 5.1:-53741227 | None:intergenic |
| CCACATGTATTTGTTGGCTT+TGG | 0.363258 | 5.1:-53736859 | None:intergenic |
| CTCAGACACGTGATATTTCT+CGG | 0.364666 | 5.1:+53738322 | MS.gene43730:CDS |
| GGAAGAGGATATTTGATTCC+TGG | 0.365318 | 5.1:-53740347 | None:intergenic |
| GGTCCCACAAATGTGGCTTC+TGG | 0.366040 | 5.1:+53735640 | MS.gene43730:CDS |
| TGGTTCCATCGGATAATTCA+TGG | 0.369194 | 5.1:+53735860 | MS.gene43730:CDS |
| CATGATTCTTCGCCACTCCC+TGG | 0.369843 | 5.1:-53736135 | None:intergenic |
| GGGGCAGGTTGCTGGGTAAC+AGG | 0.370121 | 5.1:-53740143 | None:intergenic |
| GCTGGAGGTGGGACATAAAA+TGG | 0.372492 | 5.1:-53740239 | None:intergenic |
| ATCAACATCAACCCTCTCTT+GGG | 0.382542 | 5.1:+53735923 | MS.gene43730:CDS |
| GTGGGACCATTGGCAATAAT+GGG | 0.385629 | 5.1:-53735625 | None:intergenic |
| GGAATGTAGTTTGGAGGGTA+AGG | 0.397457 | 5.1:-53740197 | None:intergenic |
| GCAGGGCTCACAATAAAGTT+TGG | 0.397497 | 5.1:-53738348 | None:intergenic |
| TTGGGTGATTGTGGATGGTC+AGG | 0.398594 | 5.1:-53738099 | None:intergenic |
| AGCTTCTGAGTGGTTTCATT+GGG | 0.399045 | 5.1:-53732086 | None:intergenic |
| ATTTCCGATTTCGCGTCTAA+TGG | 0.401669 | 5.1:-53735902 | None:intergenic |
| AAGCTTCTGAGTGGTTTCAT+TGG | 0.402439 | 5.1:-53732087 | None:intergenic |
| CTCGTGAGAGTTCGAATTCC+CGG | 0.403670 | 5.1:-53740483 | None:intergenic |
| CCACAGATTCTTCAATATCC+TGG | 0.405162 | 5.1:-53737188 | None:intergenic |
| AATGCTTAAAGAATGTTCTA+TGG | 0.405927 | 5.1:+53732061 | MS.gene43730:CDS |
| TGACCATAAGGAATGTAGTT+TGG | 0.407257 | 5.1:-53740206 | None:intergenic |
| TTGGCATTACATGGTGAGTC+AGG | 0.407647 | 5.1:-53740400 | None:intergenic |
| CGTATATATCAGCGGACAAC+AGG | 0.410693 | 5.1:+53740538 | MS.gene43730:CDS |
| GGTGCCATTAGACGCGAAAT+CGG | 0.415212 | 5.1:+53735898 | MS.gene43730:CDS |
| TCGGATAATTCATGGTTTCC+TGG | 0.418254 | 5.1:+53735868 | MS.gene43730:CDS |
| AGACCGGTTATTGTAATTAG+AGG | 0.419401 | 5.1:-53736221 | None:intergenic |
| CTCCTCCTCTGATCCAGTAC+TGG | 0.419937 | 5.1:+53735840 | MS.gene43730:CDS |
| TGCAACATTGCCATTGTACT+TGG | 0.423978 | 5.1:-53738566 | None:intergenic |
| GTCCTTCTCTTTCGTCTGCT+GGG | 0.424713 | 5.1:+53735680 | MS.gene43730:CDS |
| CCTGGGGCTGACACATTTGG+AGG | 0.425575 | 5.1:-53740329 | None:intergenic |
| TTCAACAGCTGGTTCTACTA+AGG | 0.427096 | 5.1:+53736828 | MS.gene43730:intron |
| ATATAGTAGTCACCTTGTTC+CGG | 0.429202 | 5.1:-53735231 | None:intergenic |
| TTCAAAACTGCAAAGGAAGC+TGG | 0.429461 | 5.1:+53736987 | MS.gene43730:CDS |
| CTCCACTTGGCCGGAAGACA+GGG | 0.429804 | 5.1:-53740163 | None:intergenic |
| TCTCTTTCGTCTGCTGGGAA+TGG | 0.431522 | 5.1:+53735685 | MS.gene43730:CDS |
| ATTCTACAACCTTCCACCTC+AGG | 0.432658 | 5.1:+53741142 | MS.gene43730:CDS |
| CATAATTCTGCAGCGACTAC+CGG | 0.433684 | 5.1:+53740464 | MS.gene43730:CDS |
| AATCAACATCAACCCTCTCT+TGG | 0.437546 | 5.1:+53735922 | MS.gene43730:CDS |
| CCAGGTTTGTATTGTGGAAG+AGG | 0.437588 | 5.1:-53740362 | None:intergenic |
| TTTGGAGAAATTTGCACGCC+TGG | 0.442549 | 5.1:-53738234 | None:intergenic |
| CCATATAGTCCAAAAGCATT+TGG | 0.443159 | 5.1:-53740419 | None:intergenic |
| ACTCCACTTGGCCGGAAGAC+AGG | 0.445426 | 5.1:-53740164 | None:intergenic |
| TGCATTTCCTCAACAACCTC+AGG | 0.448502 | 5.1:+53740286 | MS.gene43730:CDS |
| AGCAACCTGCCCCTGTCTTC+CGG | 0.452385 | 5.1:+53740153 | MS.gene43730:CDS |
| TTATCCGATGGAACCAGTAC+TGG | 0.452921 | 5.1:-53735853 | None:intergenic |
| GAAGACAGGGGCAGGTTGCT+GGG | 0.456269 | 5.1:-53740150 | None:intergenic |
| CCTAGTTACTTCGACTGATA+CGG | 0.457040 | 5.1:-53736920 | None:intergenic |
| ATTTCTCCAAATTACAGCTA+TGG | 0.457763 | 5.1:+53738246 | MS.gene43730:CDS |
| CTTCCTCTAATTACAATAAC+CGG | 0.460825 | 5.1:+53736218 | MS.gene43730:CDS |
| GTTCCGTGGGCAGCCTGAGT+CGG | 0.461637 | 5.1:-53741185 | None:intergenic |
| CCTGCAGCTTGAGTCGCAAC+AGG | 0.462078 | 5.1:-53740101 | None:intergenic |
| ACTCAGAAGCTTCTTCTTCA+GGG | 0.463390 | 5.1:+53732098 | MS.gene43730:CDS |
| CAAAAGCATTTGGCATTACA+TGG | 0.464966 | 5.1:-53740409 | None:intergenic |
| GCGATGTGGGTGGCTGCACC+TGG | 0.465326 | 5.1:+53741089 | MS.gene43730:CDS |
| TCATGGTTTCCTGGTGCTGT+TGG | 0.471403 | 5.1:+53735877 | MS.gene43730:CDS |
| TCTCGGTTCTTCCCAATTCA+AGG | 0.472058 | 5.1:+53740508 | MS.gene43730:CDS |
| GGAGATTGAGCATTTGGAGC+AGG | 0.474746 | 5.1:-53738595 | None:intergenic |
| GAAGAGGATATTTGATTCCT+GGG | 0.475782 | 5.1:-53740346 | None:intergenic |
| CCGTATCAGTCGAAGTAACT+AGG | 0.477477 | 5.1:+53736920 | MS.gene43730:CDS |
| GTTCCAGAAGCCACATTTGT+GGG | 0.477983 | 5.1:-53735643 | None:intergenic |
| AACCGGTCTCAACAATTGAT+TGG | 0.483215 | 5.1:+53736235 | MS.gene43730:CDS |
| AGCCACATTTGTGGGACCAT+TGG | 0.483541 | 5.1:-53735635 | None:intergenic |
| ATGATTCTTCGCCACTCCCT+GGG | 0.483592 | 5.1:-53736134 | None:intergenic |
| AATGCAACAAGCTACGTTAG+TGG | 0.488197 | 5.1:+53737123 | MS.gene43730:CDS |
| TCTGTGCAAGGAAAGATTCA+AGG | 0.488966 | 5.1:+53736106 | MS.gene43730:CDS |
| GCTGACACATTTGGAGGTGG+TGG | 0.491420 | 5.1:-53740323 | None:intergenic |
| ACCTCATTTGATTTATCGGA+AGG | 0.494113 | 5.1:-53735766 | None:intergenic |
| CAACAGTTGCTTCTGAAACT+GGG | 0.494539 | 5.1:+53735791 | MS.gene43730:CDS |
| GCCTGGGTATTGATGACCTT+CGG | 0.496749 | 5.1:-53738217 | None:intergenic |
| GTGGAACCACGCGGGAGACC+TGG | 0.496767 | 5.1:+53734647 | MS.gene43730:CDS |
| CTATATGGCTCCTCTCCAGC+TGG | 0.497010 | 5.1:+53740434 | MS.gene43730:CDS |
| CTTATGGCAGCTTCTGAGAC+TGG | 0.497242 | 5.1:+53736076 | MS.gene43730:intron |
| GAATTGGCCAAGCAATTACT+AGG | 0.497727 | 5.1:+53741334 | MS.gene43730:CDS |
| TCCTGGGATGTTTCAGAAAG+TGG | 0.497962 | 5.1:-53737171 | None:intergenic |
| CAGAATATCAAAGAGATCAC+AGG | 0.502371 | 5.1:+53732011 | MS.gene43730:CDS |
| GAATGTAGTTTGGAGGGTAA+GGG | 0.502558 | 5.1:-53740196 | None:intergenic |
| CACAGATTCTTCAATATCCT+GGG | 0.503160 | 5.1:-53737187 | None:intergenic |
| AGCTGGGCATAGTAACTAGC+TGG | 0.503589 | 5.1:-53738481 | None:intergenic |
| TATGACTTTCGCCCCGACTC+AGG | 0.504267 | 5.1:+53741172 | MS.gene43730:CDS |
| GTGGCTTCTGGAACTATTAG+TGG | 0.504498 | 5.1:+53735652 | MS.gene43730:CDS |
| AGGCAGTTACAGTCTGCGTC+TGG | 0.504814 | 5.1:+53736940 | MS.gene43730:CDS |
| GGGGCTGACACATTTGGAGG+TGG | 0.505162 | 5.1:-53740326 | None:intergenic |
| AAGAAAGAGGGACAAAAGAA+AGG | 0.505867 | 5.1:+53734188 | MS.gene43730:CDS |
| TCGAACTCTCACGAGGATCT+CGG | 0.506742 | 5.1:+53740491 | MS.gene43730:CDS |
| CTTTATTGGGTGATTGTGGA+TGG | 0.508931 | 5.1:-53738104 | None:intergenic |
| TGCAGCTGTCACTGCTTGTG+CGG | 0.509492 | 5.1:-53741228 | None:intergenic |
| TAGAATCGAGTGAGATTGAC+GGG | 0.511740 | 5.1:+53738139 | MS.gene43730:CDS |
| GGCCAATCAATTGTTGAGAC+CGG | 0.518629 | 5.1:-53736237 | None:intergenic |
| GAGTGGAGCTCATCTACCTT+CGG | 0.521935 | 5.1:+53735151 | MS.gene43730:CDS |
| CTTGGCCGGAAGACAGGGGC+AGG | 0.522527 | 5.1:-53740158 | None:intergenic |
| GTACATGAACTCCACTTGGC+CGG | 0.523164 | 5.1:-53740172 | None:intergenic |
| GCTTCTCATAATATATCTCA+TGG | 0.523993 | 5.1:+53734710 | MS.gene43730:CDS |
| AATTGGTGCATTGCTGGAGG+TGG | 0.527682 | 5.1:-53740251 | None:intergenic |
| CCTCTTCCACAATACAAACC+TGG | 0.528261 | 5.1:+53740362 | MS.gene43730:CDS |
| CTAGTTACTTCGACTGATAC+GGG | 0.529217 | 5.1:-53736919 | None:intergenic |
| AAGTAGCTTCTTCAGTGTCA+AGG | 0.529371 | 5.1:-53736966 | None:intergenic |
| CCATCATCATCAGTAACTCA+TGG | 0.530096 | 5.1:+53736175 | MS.gene43730:CDS |
| GGGAGCTGAGAACCCAACAT+TGG | 0.531833 | 5.1:-53738279 | None:intergenic |
| TTCATGAGGTCAAAAGAAAG+AGG | 0.534333 | 5.1:+53734175 | MS.gene43730:CDS |
| AAAGATTCAAGGTAAATCCC+AGG | 0.535552 | 5.1:+53736117 | MS.gene43730:CDS |
| TCTCAAACAATGGCAGGACC+TGG | 0.536492 | 5.1:+53741275 | MS.gene43730:CDS |
| TGCTGGGAATGGAGAGATAA+CGG | 0.537141 | 5.1:+53735696 | MS.gene43730:CDS |
| CCATAAGGAATGTAGTTTGG+AGG | 0.538103 | 5.1:-53740203 | None:intergenic |
| GGTACTTTATTGGGTGATTG+TGG | 0.538256 | 5.1:-53738108 | None:intergenic |
| CAGCTGGTTCTACTAAGGAA+TGG | 0.538516 | 5.1:+53736833 | MS.gene43730:intron |
| AAAGAGGGACAAAAGAAAGG+AGG | 0.538778 | 5.1:+53734191 | MS.gene43730:CDS |
| CGGTTATTGTAATTAGAGGA+AGG | 0.539416 | 5.1:-53736217 | None:intergenic |
| ATAATTCTGCAGCGACTACC+GGG | 0.541023 | 5.1:+53740465 | MS.gene43730:CDS |
| CAATTGATTGGCCCTCAGAA+AGG | 0.541403 | 5.1:+53736247 | MS.gene43730:CDS |
| GAATTATGATTATAACCAGC+TGG | 0.541828 | 5.1:-53740449 | None:intergenic |
| ACCTTCCGATAAATCAAATG+AGG | 0.542848 | 5.1:+53735765 | MS.gene43730:CDS |
| TACTGCGCCTAGTAATTGCT+TGG | 0.543688 | 5.1:-53741341 | None:intergenic |
| AATGTAGTTTGGAGGGTAAG+GGG | 0.544692 | 5.1:-53740195 | None:intergenic |
| CAATTCATCTGTGCATGCTG+AGG | 0.546216 | 5.1:-53741317 | None:intergenic |
| TTGGAGAAATTTGCACGCCT+GGG | 0.546367 | 5.1:-53738233 | None:intergenic |
| AGCACCTGGTCGGTAAAGCT+GGG | 0.546603 | 5.1:-53738497 | None:intergenic |
| CCTCCAAATGTGTCAGCCCC+AGG | 0.547907 | 5.1:+53740329 | MS.gene43730:CDS |
| CTAATGGCACCAACAGCACC+AGG | 0.548656 | 5.1:-53735886 | None:intergenic |
| AAACTTCCCCGACCACCCCT+AGG | 0.549061 | 5.1:-53734686 | None:intergenic |
| GCCCCTGTCTTCCGGCCAAG+TGG | 0.549737 | 5.1:+53740161 | MS.gene43730:CDS |
| AACTGGGTCGGCATCGTCAT+CGG | 0.551030 | 5.1:+53735807 | MS.gene43730:CDS |
| AGTTCCAGAAGCCACATTTG+TGG | 0.551364 | 5.1:-53735644 | None:intergenic |
| GAGATTGTTGTAGCAACGGA+TGG | 0.553068 | 5.1:-53741256 | None:intergenic |
| TTAGAATCGAGTGAGATTGA+CGG | 0.553569 | 5.1:+53738138 | MS.gene43730:CDS |
| CATAAGGAATGTAGTTTGGA+GGG | 0.554153 | 5.1:-53740202 | None:intergenic |
| GTAGCCCCAGCAGAAGCAAA+AGG | 0.554741 | 5.1:-53738541 | None:intergenic |
| TCCGTGGGCAGCCTGAGTCG+GGG | 0.556072 | 5.1:-53741183 | None:intergenic |
| CAACAATCTCAAACAATGGC+AGG | 0.556793 | 5.1:+53741269 | MS.gene43730:CDS |
| AAATTAGTCATGTCTCGGCC+AGG | 0.558993 | 5.1:-53741107 | None:intergenic |
| GATTGAGCATTTGGAGCAGG+TGG | 0.559667 | 5.1:-53738592 | None:intergenic |
| AACATTGCCATTGTACTTGG+TGG | 0.559871 | 5.1:-53738563 | None:intergenic |
| GCCCCGACTCAGGCTGCCCA+CGG | 0.563199 | 5.1:+53741182 | MS.gene43730:CDS |
| GCCAAGAACAAATTAACTGC+CGG | 0.563373 | 5.1:+53735967 | MS.gene43730:CDS |
| GAGGAAGGGCGACTAACTGA+AGG | 0.568207 | 5.1:-53736202 | None:intergenic |
| CCTGTTGCGACTCAAGCTGC+AGG | 0.568319 | 5.1:+53740101 | MS.gene43730:CDS |
| CAGAAGCAAAAGGAGAAAGA+CGG | 0.569551 | 5.1:-53738531 | None:intergenic |
| AACACTGCTTTGCCGTCCGA+AGG | 0.570438 | 5.1:+53738201 | MS.gene43730:CDS |
| TCTCAAGAAATAGTATCTGA+AGG | 0.570506 | 5.1:+53735200 | MS.gene43730:CDS |
| CCATGAGTTACTGATGATGA+TGG | 0.570613 | 5.1:-53736175 | None:intergenic |
| CTGTGGAGGAGCAGAACTCA+AGG | 0.570858 | 5.1:+53737205 | MS.gene43730:CDS |
| TCTGCGCCCATTATTGCCAA+TGG | 0.571659 | 5.1:+53735619 | MS.gene43730:CDS |
| TTCTACAACCTTCCACCTCA+GGG | 0.571700 | 5.1:+53741143 | MS.gene43730:CDS |
| AGCATTTGATTCCCCAAGAG+AGG | 0.572579 | 5.1:-53735935 | None:intergenic |
| GTGCTTCCCTGAGGTTGTTG+AGG | 0.573411 | 5.1:-53740293 | None:intergenic |
| CTGTCACTGCTTGTGCGGGG+TGG | 0.573678 | 5.1:-53741223 | None:intergenic |
| TGTTGATAAACATTTCCTCC+AGG | 0.575150 | 5.1:-53741293 | None:intergenic |
| AAATGCTCAATCTCCTCAAG+AGG | 0.577511 | 5.1:+53738603 | MS.gene43730:CDS |
| GCATTTGATTCCCCAAGAGA+GGG | 0.578799 | 5.1:-53735934 | None:intergenic |
| TGCCAATGGTCCCACAAATG+TGG | 0.583114 | 5.1:+53735633 | MS.gene43730:CDS |
| GCTACAACAATCTCAAACAA+TGG | 0.584988 | 5.1:+53741265 | MS.gene43730:CDS |
| ATGTAGTTTGGAGGGTAAGG+GGG | 0.585061 | 5.1:-53740194 | None:intergenic |
| TAACTAGCTGGATCATATTG+TGG | 0.585179 | 5.1:-53738469 | None:intergenic |
| CAATAATACTAATAATAATG+TGG | 0.585330 | 5.1:+53735732 | MS.gene43730:CDS |
| AGTAGCTTCTTCAGTGTCAA+GGG | 0.585388 | 5.1:-53736965 | None:intergenic |
| GTATCAATGCAAACCTCTTG+AGG | 0.587331 | 5.1:-53738616 | None:intergenic |
| TCAAGGTAAATCCCAGGGAG+TGG | 0.590490 | 5.1:+53736123 | MS.gene43730:CDS |
| AAGGTTGTAGAATGAAGTAG+TGG | 0.591342 | 5.1:-53741132 | None:intergenic |
| AAATGGTGCACTTACGTCTG+AGG | 0.591740 | 5.1:+53738065 | MS.gene43730:CDS |
| CAGCTGTCACTGCTTGTGCG+GGG | 0.592680 | 5.1:-53741226 | None:intergenic |
| CCAGGATATTGAAGAATCTG+TGG | 0.593048 | 5.1:+53737188 | MS.gene43730:CDS |
| AGATGCTTGCATAAGTTCCG+TGG | 0.593203 | 5.1:-53741199 | None:intergenic |
| AAGCTACTTCAAAACTGCAA+AGG | 0.593709 | 5.1:+53736980 | MS.gene43730:CDS |
| TGATCCAGTACTGGTTCCAT+CGG | 0.595469 | 5.1:+53735849 | MS.gene43730:CDS |
| GAGTGAAGGTTCAGCGATGT+GGG | 0.596741 | 5.1:+53741076 | MS.gene43730:intron |
| TATGCCCAGCTTTACCGACC+AGG | 0.596802 | 5.1:+53738493 | MS.gene43730:CDS |
| TGGGCAAATTAGTCATGTCT+CGG | 0.597854 | 5.1:-53741112 | None:intergenic |
| GTTTGAGATTGTTGTAGCAA+CGG | 0.600037 | 5.1:-53741260 | None:intergenic |
| ACCACTTTCTGAAACATCCC+AGG | 0.600974 | 5.1:+53737170 | MS.gene43730:CDS |
| GTCATATGCTGACCCTGAGG+TGG | 0.601191 | 5.1:-53741155 | None:intergenic |
| TTTGTTCCAGGTTTGTATTG+TGG | 0.601290 | 5.1:-53740368 | None:intergenic |
| GCTGTTACAGGCAAAGAGAG+TGG | 0.601661 | 5.1:+53735134 | MS.gene43730:CDS |
| ATGATTATAACCAGCTGGAG+AGG | 0.603104 | 5.1:-53740444 | None:intergenic |
| CGGCCGTCACTATCAGCACC+TGG | 0.604561 | 5.1:-53738511 | None:intergenic |
| TCATGAGGTCAAAAGAAAGA+GGG | 0.607899 | 5.1:+53734176 | MS.gene43730:CDS |
| ACTGGTAGCTCTTCTGTGCA+AGG | 0.609421 | 5.1:+53736094 | MS.gene43730:CDS |
| TGTGGTGCAACTTTCTCCGA+AGG | 0.612189 | 5.1:-53735167 | None:intergenic |
| AGTTGCTTCTGAAACTGGGT+CGG | 0.612810 | 5.1:+53735795 | MS.gene43730:CDS |
| GACCTTCTCTCAGATACTAG+TGG | 0.613658 | 5.1:+53735101 | MS.gene43730:intron |
| TCTCAACAACAAAGAACATG+TGG | 0.616833 | 5.1:+53734628 | MS.gene43730:CDS |
| GGTTATTGTAATTAGAGGAA+GGG | 0.617353 | 5.1:-53736216 | None:intergenic |
| TCACTCGATTCTAAATTCAC+AGG | 0.621424 | 5.1:-53738129 | None:intergenic |
| TCCACTTGGCCGGAAGACAG+GGG | 0.624961 | 5.1:-53740162 | None:intergenic |
| TGGTGCACTTACGTCTGAGG+TGG | 0.627284 | 5.1:+53738068 | MS.gene43730:CDS |
| TCAACATCAACCCTCTCTTG+GGG | 0.628659 | 5.1:+53735924 | MS.gene43730:CDS |
| GAAAATAACGTATATATCAG+CGG | 0.629067 | 5.1:+53740530 | MS.gene43730:CDS |
| TCCGAAGGTCATCAATACCC+AGG | 0.630730 | 5.1:+53738216 | MS.gene43730:CDS |
| AGAGTGAAGGTTCAGCGATG+TGG | 0.631509 | 5.1:+53741075 | MS.gene43730:intron |
| GATGCTTGCATAAGTTCCGT+GGG | 0.631805 | 5.1:-53741198 | None:intergenic |
| TGGAACCAGTACTGGATCAG+AGG | 0.632149 | 5.1:-53735845 | None:intergenic |
| AGAATATCAAAGAGATCACA+GGG | 0.635001 | 5.1:+53732012 | MS.gene43730:CDS |
| GCATTTCCTCAACAACCTCA+GGG | 0.638456 | 5.1:+53740287 | MS.gene43730:CDS |
| AAGATTCAAGGTAAATCCCA+GGG | 0.641814 | 5.1:+53736118 | MS.gene43730:CDS |
| TATGCTGACCCTGAGGTGGA+AGG | 0.644820 | 5.1:-53741151 | None:intergenic |
| AGGAAGGGCGACTAACTGAA+GGG | 0.645153 | 5.1:-53736201 | None:intergenic |
| GGAGGTATCTTGTCCACAGT+AGG | 0.645987 | 5.1:+53740071 | MS.gene43730:CDS |
| GGGGCTACCACCAAGTACAA+TGG | 0.647638 | 5.1:+53738556 | MS.gene43730:CDS |
| AACCAGTACTGGATCAGAGG+AGG | 0.653183 | 5.1:-53735842 | None:intergenic |
| GGGTATTGATGACCTTCGGA+CGG | 0.653740 | 5.1:-53738213 | None:intergenic |
| ATTGGTGCATTGCTGGAGGT+GGG | 0.656216 | 5.1:-53740250 | None:intergenic |
| AGTATAACGTGTTGACGCTG+TGG | 0.657592 | 5.1:-53737024 | None:intergenic |
| TTCCCAGCAGACGAAAGAGA+AGG | 0.658186 | 5.1:-53735682 | None:intergenic |
| GGAAACCATGAATTATCCGA+TGG | 0.660367 | 5.1:-53735865 | None:intergenic |
| CAAACAATGGCAGGACCTGG+AGG | 0.664067 | 5.1:+53741278 | MS.gene43730:CDS |
| CGGGAATTCGAACTCTCACG+AGG | 0.664119 | 5.1:+53740484 | MS.gene43730:CDS |
| GGATATTGAAGAATCTGTGG+AGG | 0.664638 | 5.1:+53737191 | MS.gene43730:CDS |
| AACAGGTGTTTGACCTACTG+TGG | 0.669264 | 5.1:-53740084 | None:intergenic |
| GTTACTGATGATGATGGAGA+TGG | 0.671333 | 5.1:-53736169 | None:intergenic |
| AAGAGGATATTTGATTCCTG+GGG | 0.672575 | 5.1:-53740345 | None:intergenic |
| AGCTGAGAACCCAACATTGG+GGG | 0.673015 | 5.1:-53738276 | None:intergenic |
| CGTCACTATCAGCACCTGGT+CGG | 0.674868 | 5.1:-53738507 | None:intergenic |
| CGACCAGGTGCTGATAGTGA+CGG | 0.681365 | 5.1:+53738508 | MS.gene43730:CDS |
| GAGCTGAGAACCCAACATTG+GGG | 0.682241 | 5.1:-53738277 | None:intergenic |
| CCAAAGCCAACAAATACATG+TGG | 0.688331 | 5.1:+53736859 | MS.gene43730:CDS |
| AGGTTGTAGAATGAAGTAGT+GGG | 0.688484 | 5.1:-53741131 | None:intergenic |
| GGATGTTTCAGAAAGTGGTG+TGG | 0.689678 | 5.1:-53737166 | None:intergenic |
| CTGAAGAAGAAGCTTCTGAG+TGG | 0.689817 | 5.1:-53732096 | None:intergenic |
| TCTTGAGAAGCTGATAAATG+TGG | 0.691478 | 5.1:-53735185 | None:intergenic |
| AAGAACATGTGGAACCACGC+GGG | 0.698749 | 5.1:+53734639 | MS.gene43730:CDS |
| TGAAGGTTCAGCGATGTGGG+TGG | 0.713947 | 5.1:+53741079 | MS.gene43730:CDS |
| AAAGTCATATGCTGACCCTG+AGG | 0.718391 | 5.1:-53741158 | None:intergenic |
| AAAGAACATGTGGAACCACG+CGG | 0.723349 | 5.1:+53734638 | MS.gene43730:CDS |
| GGATATATAGTGCTTCCCTG+AGG | 0.730562 | 5.1:-53740302 | None:intergenic |
| GTTACTTCGACTGATACGGG+AGG | 0.745635 | 5.1:-53736916 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 53731957 | 53741355 | 53731957 | ID=MS.gene43730 |
| chr5.1 | mRNA | 53731957 | 53741355 | 53731957 | ID=MS.gene43730.t1;Parent=MS.gene43730 |
| chr5.1 | exon | 53731957 | 53732119 | 53731957 | ID=MS.gene43730.t1.exon1;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53731957 | 53732119 | 53731957 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53734169 | 53734212 | 53734169 | ID=MS.gene43730.t1.exon2;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53734169 | 53734212 | 53734169 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53734626 | 53734731 | 53734626 | ID=MS.gene43730.t1.exon3;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53734626 | 53734731 | 53734626 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53735114 | 53735240 | 53735114 | ID=MS.gene43730.t1.exon4;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53735114 | 53735240 | 53735114 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53735618 | 53735988 | 53735618 | ID=MS.gene43730.t1.exon5;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53735618 | 53735988 | 53735618 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53736086 | 53736268 | 53736086 | ID=MS.gene43730.t1.exon6;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53736086 | 53736268 | 53736086 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53736836 | 53737226 | 53736836 | ID=MS.gene43730.t1.exon7;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53736836 | 53737226 | 53736836 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53738062 | 53738359 | 53738062 | ID=MS.gene43730.t1.exon8;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53738062 | 53738359 | 53738062 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53738463 | 53738624 | 53738463 | ID=MS.gene43730.t1.exon9;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53738463 | 53738624 | 53738463 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53740071 | 53740559 | 53740071 | ID=MS.gene43730.t1.exon10;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53740071 | 53740559 | 53740071 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| chr5.1 | exon | 53741077 | 53741355 | 53741077 | ID=MS.gene43730.t1.exon11;Parent=MS.gene43730.t1 |
| chr5.1 | CDS | 53741077 | 53741355 | 53741077 | ID=cds.MS.gene43730.t1;Parent=MS.gene43730.t1 |
| Gene Sequence |
| Protein sequence |
