AlfalfaGEDB | MS.gene44123

M. sativa cultivar XinJiangDaYe / MS.gene44123

KEGG: K13447
GO: GO:0003674 GO:0003824 GO:0004601 GO:0008150 GO:0008152 GO:0016209 GO:0016491 GO:0016651 GO:0016684 GO:0050664 GO:0055114
PFAM: PF08414.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44123.t1	XP_003591142.1	88.4	172	15	2	1	171	1	168	3.60E-71	277.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44123.t1	Q948T9	64.0	161	42	2	15	170	8	157	9.4e-44	177.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44123.t1	G7I8P3	88.4	172	15	2	1	171	1	168	2.6e-71	277.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056059	MS.gene44123	0.814893	1.22E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44123.t1	MTR_1g083290	92.442	172	8	2	1	171	1	168	2.41e-101	310
MS.gene44123.t1	MTR_7g113130	71.271	181	42	5	1	171	1	181	5.69e-74	235
MS.gene44123.t1	MTR_7g113130	71.271	181	42	5	1	171	1	181	8.44e-73	234
MS.gene44123.t1	MTR_7g113130	71.271	181	42	5	1	171	1	181	1.11e-72	234
MS.gene44123.t1	MTR_3g098350	34.940	166	84	5	29	170	10	175	1.86e-19	85.1
MS.gene44123.t1	MTR_8g095520	33.571	140	76	4	49	171	40	179	2.38e-19	84.7
MS.gene44123.t1	MTR_3g098380	32.203	177	85	7	26	171	28	200	1.67e-18	82.4
MS.gene44123.t1	MTR_3g098320	37.736	159	85	6	21	170	21	174	2.00e-18	82.0
MS.gene44123.t1	MTR_3g098320	37.736	159	85	6	21	170	21	174	2.48e-18	81.6
MS.gene44123.t1	MTR_3g098320	37.736	159	85	6	21	170	21	174	2.88e-18	81.6
MS.gene44123.t1	MTR_3g098320	36.943	157	85	6	21	168	21	172	5.56e-17	77.8
MS.gene44123.t1	MTR_3g098320	36.943	157	85	6	21	168	21	172	8.42e-17	77.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44123.t1	AT5G51060	36.994	173	85	6	22	171	16	187	1.60e-24	99.8
MS.gene44123.t1	AT5G47910	38.503	187	81	10	17	171	28	212	3.60e-20	87.0
MS.gene44123.t1	AT1G09090	43.307	127	50	5	47	170	23	130	9.78e-20	85.9
MS.gene44123.t1	AT1G09090	43.307	127	50	5	47	170	23	130	1.18e-19	85.5
MS.gene44123.t1	AT5G07390	36.986	146	70	4	47	170	36	181	2.55e-15	73.2
MS.gene44123.t1	AT5G07390	36.986	146	70	4	47	170	36	181	3.30e-15	72.8
MS.gene44123.t1	AT3G45810	44.828	58	29	1	116	170	106	163	5.85e-11	60.5
MS.gene44123.t1	AT5G60010	44.828	58	29	1	116	170	96	153	6.66e-11	60.5

Find 42 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACACCGAAACGGCGTTTC+TGG	0.250511	1.3:+58814698	MS.gene44123:CDS
CATCAAAACGCTTCTCAACT+TGG	0.366463	1.3:-58814902	None:intergenic
GAAGCGTCCGTCGTCGCTTT+CGG	0.373980	1.3:+58814734	MS.gene44123:CDS
CTTCGGCTTTCTGTGCTCTC+TGG	0.375928	1.3:-58814510	None:intergenic
GCTTCAGCGGGCCGCTAAGC+GGG	0.384442	1.3:+58814544	MS.gene44123:CDS
CACTGGCTAAATCGAGTCTT+GGG	0.426185	1.3:-58814948	None:intergenic
AGCTCACGGGTGGGCTTCAG+CGG	0.449072	1.3:+58814531	MS.gene44123:CDS
GATGCTAAAGGCCCGCTTAG+CGG	0.450959	1.3:-58814555	None:intergenic
GCTTGCCAGAAACGCCGTTT+CGG	0.451321	1.3:-58814703	None:intergenic
CTTCAAGGATGACGGTGAAA+TGG	0.466601	1.3:+58814617	MS.gene44123:CDS
AAGGTTGATAGATCAAAATC+TGG	0.469714	1.3:+58814816	MS.gene44123:CDS
TAAACTAAGACAGGTGTCTC+AGG	0.471090	1.3:+58814758	MS.gene44123:CDS
GGCTTCAGCGGGCCGCTAAG+CGG	0.473400	1.3:+58814543	MS.gene44123:CDS
TGAAGCCCACCCGTGAGCTT+CGG	0.474667	1.3:-58814527	None:intergenic
GAACAAAAGTGGAAGCTTCA+AGG	0.484269	1.3:+58814602	MS.gene44123:CDS
GCACTGGCTAAATCGAGTCT+TGG	0.494740	1.3:-58814949	None:intergenic
AACCGAAGCAAGAACAAAAG+TGG	0.503450	1.3:+58814591	MS.gene44123:CDS
CACAGAAAGCCGAAGCTCAC+GGG	0.516919	1.3:+58814518	MS.gene44123:CDS
GGTGCTGCTCGTGCTCTACA+AGG	0.517296	1.3:+58814837	MS.gene44123:CDS
ATCAAAACGCTTCTCAACTT+GGG	0.524127	1.3:-58814901	None:intergenic
GCTCACGGGTGGGCTTCAGC+GGG	0.534753	1.3:+58814532	MS.gene44123:CDS
GCACAGAAAGCCGAAGCTCA+CGG	0.538948	1.3:+58814517	MS.gene44123:CDS
AAACGGCGTTTCTGGCAAGC+CGG	0.551405	1.3:+58814706	MS.gene44123:CDS
GTTTCCGTTCAGAATATTCG+CGG	0.564937	1.3:+58814672	MS.gene44123:CDS
ACATGGACATACCTATGCAC+TGG	0.571320	1.3:-58814965	None:intergenic
AGAAAGCCGAAGCTCACGGG+TGG	0.572651	1.3:+58814521	MS.gene44123:CDS
GCTTTCGGCTAAACTAAGAC+AGG	0.576796	1.3:+58814749	MS.gene44123:CDS
TTTGATGAGTTGGCTGTGGA+GGG	0.587990	1.3:+58814918	MS.gene44123:CDS
TCGCGGTGGAGACACCGAAA+CGG	0.593743	1.3:+58814689	MS.gene44123:CDS
ACGACGGACGCTTCTCAAGC+CGG	0.596085	1.3:-58814725	None:intergenic
AGTTTAGCCGAAAGCGACGA+CGG	0.602465	1.3:-58814741	None:intergenic
AGTGGAAGCTTCAAGGATGA+CGG	0.607876	1.3:+58814609	MS.gene44123:CDS
CAAGGATGACGGTGAAATGG+TGG	0.608567	1.3:+58814620	MS.gene44123:CDS
AAGAGAGCAAACATGAGTCA+TGG	0.614927	1.3:+58814481	MS.gene44123:CDS
AAAACGTTGGAAGTGAAGGT+TGG	0.620114	1.3:+58814877	MS.gene44123:CDS
ACTCGATTTAGCCAGTGCAT+AGG	0.626507	1.3:+58814954	MS.gene44123:CDS
GAGCAAACATGAGTCATGGT+CGG	0.629365	1.3:+58814485	MS.gene44123:CDS
GAAAGCCGAAGCTCACGGGT+GGG	0.641161	1.3:+58814522	MS.gene44123:CDS
TCCGTTCAGAATATTCGCGG+TGG	0.679005	1.3:+58814675	MS.gene44123:CDS
TCCACCGCGAATATTCTGAA+CGG	0.684589	1.3:-58814676	None:intergenic
TGAAATGGTGGAGATAACGT+TGG	0.685098	1.3:+58814632	MS.gene44123:CDS
ATGAAGCATAGACTATAACA+TGG	0.703673	1.3:-58814982	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTTTATGACAAAAAACGT+TGG	+	chr1.3:58814864-58814883	MS.gene44123:CDS	25.0%
!!	AAGGTTGATAGATCAAAATC+TGG	+	chr1.3:58814816-58814835	MS.gene44123:CDS	30.0%
	ACAAAAAACGTTGGAAGTGA+AGG	+	chr1.3:58814873-58814892	MS.gene44123:CDS	35.0%
	ATCAAAACGCTTCTCAACTT+GGG	-	chr1.3:58814904-58814923	None:intergenic	35.0%
!!	TTCCACTTTTGTTCTTGCTT+CGG	-	chr1.3:58814596-58814615	None:intergenic	35.0%
!!!	GTCTTTTAAGGCGTTTGATA+AGG	+	chr1.3:58814797-58814816	MS.gene44123:CDS	35.0%
	AAAACGTTGGAAGTGAAGGT+TGG	+	chr1.3:58814877-58814896	MS.gene44123:CDS	40.0%
	AACCGAAGCAAGAACAAAAG+TGG	+	chr1.3:58814591-58814610	MS.gene44123:CDS	40.0%
	AAGAGAGCAAACATGAGTCA+TGG	+	chr1.3:58814481-58814500	MS.gene44123:CDS	40.0%
	CATCAAAACGCTTCTCAACT+TGG	-	chr1.3:58814905-58814924	None:intergenic	40.0%
	GAACAAAAGTGGAAGCTTCA+AGG	+	chr1.3:58814602-58814621	MS.gene44123:CDS	40.0%
	GTTTCCGTTCAGAATATTCG+CGG	+	chr1.3:58814672-58814691	MS.gene44123:CDS	40.0%
	TAAACTAAGACAGGTGTCTC+AGG	+	chr1.3:58814758-58814777	MS.gene44123:CDS	40.0%
!	TGAAATGGTGGAGATAACGT+TGG	+	chr1.3:58814632-58814651	MS.gene44123:CDS	40.0%
!!!	CGCTGTTTTTTGGATGCTAA+AGG	-	chr1.3:58814570-58814589	None:intergenic	40.0%
!!!	TGAGAAGCGTTTTGATGAGT+TGG	+	chr1.3:58814908-58814927	MS.gene44123:CDS	40.0%
	ACATGGACATACCTATGCAC+TGG	-	chr1.3:58814968-58814987	None:intergenic	45.0%
	ACTCGATTTAGCCAGTGCAT+AGG	+	chr1.3:58814954-58814973	MS.gene44123:CDS	45.0%
	AGTGGAAGCTTCAAGGATGA+CGG	+	chr1.3:58814609-58814628	MS.gene44123:CDS	45.0%
	GAGCAAACATGAGTCATGGT+CGG	+	chr1.3:58814485-58814504	MS.gene44123:CDS	45.0%
	GCTTTCGGCTAAACTAAGAC+AGG	+	chr1.3:58814749-58814768	MS.gene44123:CDS	45.0%
	TCCACCGCGAATATTCTGAA+CGG	-	chr1.3:58814679-58814698	None:intergenic	45.0%
!	CACTGGCTAAATCGAGTCTT+GGG	-	chr1.3:58814951-58814970	None:intergenic	45.0%
!	CTTCAAGGATGACGGTGAAA+TGG	+	chr1.3:58814617-58814636	MS.gene44123:CDS	45.0%
!	GAAGCGCATGACGTCTTTTA+AGG	+	chr1.3:58814785-58814804	MS.gene44123:CDS	45.0%
!	TTTGATGAGTTGGCTGTGGA+GGG	+	chr1.3:58814918-58814937	MS.gene44123:CDS	45.0%
!!	TTTTGATGAGTTGGCTGTGG+AGG	+	chr1.3:58814917-58814936	MS.gene44123:CDS	45.0%
!!!	GCTTCGGTTTCGCTGTTTTT+TGG	-	chr1.3:58814580-58814599	None:intergenic	45.0%
	AGTTTAGCCGAAAGCGACGA+CGG	-	chr1.3:58814744-58814763	None:intergenic	50.0%
	TCCGTTCAGAATATTCGCGG+TGG	+	chr1.3:58814675-58814694	MS.gene44123:CDS	50.0%
!	CAAGGATGACGGTGAAATGG+TGG	+	chr1.3:58814620-58814639	MS.gene44123:CDS	50.0%
!!	GCACTGGCTAAATCGAGTCT+TGG	-	chr1.3:58814952-58814971	None:intergenic	50.0%
!!	GCGTTTTGATGAGTTGGCTG+TGG	+	chr1.3:58814914-58814933	MS.gene44123:CDS	50.0%
	AGACACCGAAACGGCGTTTC+TGG	+	chr1.3:58814698-58814717	MS.gene44123:CDS	55.0%
	CACAGAAAGCCGAAGCTCAC+GGG	+	chr1.3:58814518-58814537	MS.gene44123:CDS	55.0%
	CTTCGGCTTTCTGTGCTCTC+TGG	-	chr1.3:58814513-58814532	None:intergenic	55.0%
	GATGCTAAAGGCCCGCTTAG+CGG	-	chr1.3:58814558-58814577	None:intergenic	55.0%
	GCACAGAAAGCCGAAGCTCA+CGG	+	chr1.3:58814517-58814536	MS.gene44123:CDS	55.0%
	GCTTGCCAGAAACGCCGTTT+CGG	-	chr1.3:58814706-58814725	None:intergenic	55.0%
!!	AAACGGCGTTTCTGGCAAGC+CGG	+	chr1.3:58814706-58814725	MS.gene44123:CDS	55.0%
	ACGACGGACGCTTCTCAAGC+CGG	-	chr1.3:58814728-58814747	None:intergenic	60.0%
	AGAAAGCCGAAGCTCACGGG+TGG	+	chr1.3:58814521-58814540	MS.gene44123:CDS	60.0%
	GAAAGCCGAAGCTCACGGGT+GGG	+	chr1.3:58814522-58814541	MS.gene44123:CDS	60.0%
	TCGCGGTGGAGACACCGAAA+CGG	+	chr1.3:58814689-58814708	MS.gene44123:CDS	60.0%
	TGAAGCCCACCCGTGAGCTT+CGG	-	chr1.3:58814530-58814549	None:intergenic	60.0%
!	GAAGCGTCCGTCGTCGCTTT+CGG	+	chr1.3:58814734-58814753	MS.gene44123:CDS	60.0%
!!	GGTGCTGCTCGTGCTCTACA+AGG	+	chr1.3:58814837-58814856	MS.gene44123:CDS	60.0%
!	AGCTCACGGGTGGGCTTCAG+CGG	+	chr1.3:58814531-58814550	MS.gene44123:CDS	65.0%
	GCTTCAGCGGGCCGCTAAGC+GGG	+	chr1.3:58814544-58814563	MS.gene44123:CDS	70.0%
	GGCTTCAGCGGGCCGCTAAG+CGG	+	chr1.3:58814543-58814562	MS.gene44123:CDS	70.0%
!	GCTCACGGGTGGGCTTCAGC+GGG	+	chr1.3:58814532-58814551	MS.gene44123:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	58814471	58814992	58814471	ID=MS.gene44123
chr1.3	mRNA	58814471	58814992	58814471	ID=MS.gene44123.t1;Parent=MS.gene44123
chr1.3	exon	58814471	58814992	58814471	ID=MS.gene44123.t1.exon1;Parent=MS.gene44123.t1
chr1.3	CDS	58814471	58814992	58814471	ID=cds.MS.gene44123.t1;Parent=MS.gene44123.t1

Gene Sequence

>MS.gene44123

ATGGAAGTTCAAGAGAGCAAACATGAGTCATGGTCGGAGCCAGAGAGCACAGAAAGCCGAAGCTCACGGGTGGGCTTCAGCGGGCCGCTAAGCGGGCCTTTAGCATCCAAAAAACAGCGAAACCGAAGCAAGAACAAAAGTGGAAGCTTCAAGGATGACGGTGAAATGGTGGAGATAACGTTGGACGTACGTGATGACTCTGTTTCCGTTCAGAATATTCGCGGTGGAGACACCGAAACGGCGTTTCTGGCAAGCCGGCTTGAGAAGCGTCCGTCGTCGCTTTCGGCTAAACTAAGACAGGTGTCTCAGGAGCTGAAGCGCATGACGTCTTTTAAGGCGTTTGATAAGGTTGATAGATCAAAATCTGGTGCTGCTCGTGCTCTACAAGGTCTTAAGTTTATGACAAAAAACGTTGGAAGTGAAGGTTGGTCCCAAGTTGAGAAGCGTTTTGATGAGTTGGCTGTGGAGGGAAAATTGCCCAAGACTCGATTTAGCCAGTGCATAGGTATGTCCATGTTATAG

Protein sequence

>MS.gene44123.t1

MEVQESKHESWSEPESTESRSSRVGFSGPLSGPLASKKQRNRSKNKSGSFKDDGEMVEITLDVRDDSVSVQNIRGGDTETAFLASRLEKRPSSLSAKLRQVSQELKRMTSFKAFDKVDRSKSGAARALQGLKFMTKNVGSEGWSQVEKRFDELAVEGKLPKTRFSQCIGMSML