Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44137.t1 | XP_003591157.1 | 97.8 | 90 | 0 | 1 | 1 | 88 | 1 | 90 | 3.80E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44137.t1 | G7I8Q8 | 97.8 | 90 | 0 | 1 | 1 | 88 | 1 | 90 | 2.7e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056071 | MS.gene44137 | 0.910857 | 1.17E-82 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44137.t1 | MTR_1g083420 | 97.778 | 90 | 0 | 1 | 1 | 88 | 1 | 90 | 2.16e-59 | 176 |
| MS.gene44137.t1 | MTR_3g072170 | 46.575 | 73 | 38 | 1 | 8 | 79 | 1 | 73 | 1.99e-18 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44137.t1 | AT3G05550 | 83.544 | 79 | 13 | 0 | 1 | 79 | 1 | 79 | 8.00e-34 | 112 |
| MS.gene44137.t1 | AT5G27760 | 78.205 | 78 | 17 | 0 | 1 | 78 | 1 | 78 | 1.14e-31 | 106 |
| MS.gene44137.t1 | AT5G27760 | 72.222 | 54 | 15 | 0 | 1 | 54 | 1 | 54 | 6.60e-27 | 94.0 |
Find 22 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCCTGAGAGATTCGATT+TGG | 0.327609 | 1.3:-59005942 | None:intergenic |
| AAATCGAATCTCTCAGGAAA+TGG | 0.335949 | 1.3:+59005944 | MS.gene44137:CDS |
| AAAAGGCTGCAAGGGATTCT+AGG | 0.341872 | 1.3:+59007688 | MS.gene44137:CDS |
| TTGTGGCTTAGCGGTATTAC+GGG | 0.371274 | 1.3:+59006108 | MS.gene44137:CDS |
| TTTGTGGCTTAGCGGTATTA+CGG | 0.376845 | 1.3:+59006107 | MS.gene44137:CDS |
| CGGGTTCAATTGCGTACAAT+TGG | 0.387745 | 1.3:+59006127 | MS.gene44137:CDS |
| CTTACACTGGGAGCATTAGC+TGG | 0.431990 | 1.3:+59007627 | MS.gene44137:CDS |
| TTTCCTGAGAGATTCGATTT+GGG | 0.436962 | 1.3:-59005941 | None:intergenic |
| TGATCACAAGTCTGAAGAAA+AGG | 0.467242 | 1.3:+59007671 | MS.gene44137:CDS |
| TTTCTCGATTAGGTTGTTTG+TGG | 0.469318 | 1.3:+59006091 | MS.gene44137:intron |
| AATCGAATCTCTCAGGAAAT+GGG | 0.471588 | 1.3:+59005945 | MS.gene44137:CDS |
| AAACCCAAATCGAATCTCTC+AGG | 0.502597 | 1.3:+59005938 | MS.gene44137:CDS |
| GCATGCTCAAGCTCTTACAC+TGG | 0.522809 | 1.3:+59007614 | MS.gene44137:CDS |
| GTCTGAAGAAAAGGCTGCAA+GGG | 0.524443 | 1.3:+59007680 | MS.gene44137:CDS |
| TTAGGTTGTTTGTGGCTTAG+CGG | 0.532960 | 1.3:+59006099 | MS.gene44137:intron |
| GTGTTAAGATTATTCACGCC+AGG | 0.537077 | 1.3:+59006172 | MS.gene44137:CDS |
| AGCATTAGCTGGAGCTGCAG+TGG | 0.578637 | 1.3:+59007638 | MS.gene44137:CDS |
| CATGCTCAAGCTCTTACACT+GGG | 0.582325 | 1.3:+59007615 | MS.gene44137:CDS |
| AGTCTGAAGAAAAGGCTGCA+AGG | 0.589757 | 1.3:+59007679 | MS.gene44137:CDS |
| GAACACAAACTCCGTACTGT+TGG | 0.612045 | 1.3:+59005973 | MS.gene44137:CDS |
| ATGGAAATTTACCAACAGTA+CGG | 0.620884 | 1.3:-59005984 | None:intergenic |
| GGCGTGAATAATCTTAACAC+TGG | 0.626108 | 1.3:-59006169 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATATTATTATTATTATTT+TGG | + | chr1.3:59006503-59006522 | MS.gene44137:intron | 0.0% |
| !! | ATCATATCATCATAAAATAA+AGG | - | chr1.3:59006030-59006049 | None:intergenic | 15.0% |
| !! | TATATATTTACTTGAATTGA+TGG | - | chr1.3:59006359-59006378 | None:intergenic | 15.0% |
| !!! | AATGAATTTTATACAGTTTA+GGG | - | chr1.3:59006391-59006410 | None:intergenic | 15.0% |
| !!! | ATTTTATGATGATATGATTT+AGG | + | chr1.3:59006032-59006051 | MS.gene44137:intron | 15.0% |
| !!! | TAATGAATTTTATACAGTTT+AGG | - | chr1.3:59006392-59006411 | None:intergenic | 15.0% |
| !! | ACAATAATAATTAACAAACC+TGG | - | chr1.3:59006193-59006212 | None:intergenic | 20.0% |
| !! | TATCATCATAAAATAAAGGA+AGG | - | chr1.3:59006026-59006045 | None:intergenic | 20.0% |
| !!! | AAATTCTTCTTTTTGGTAAA+CGG | + | chr1.3:59006973-59006992 | MS.gene44137:intron | 20.0% |
| !!! | TTCAAGTTAGAATCTATTTT+GGG | + | chr1.3:59006798-59006817 | MS.gene44137:intron | 20.0% |
| !!! | TTTAATTTGTTTTCTCGATT+AGG | + | chr1.3:59006081-59006100 | MS.gene44137:intron | 20.0% |
| !!! | TTTTTGAGTTATTTTGTATC+AGG | + | chr1.3:59007589-59007608 | MS.gene44137:intron | 20.0% |
| ! | AATAAAAAGGATACACTTCA+AGG | - | chr1.3:59007236-59007255 | None:intergenic | 25.0% |
| ! | AGGAAATTGATTGATTGATA+TGG | - | chr1.3:59006006-59006025 | None:intergenic | 25.0% |
| ! | ATAAAAAGGATACACTTCAA+GGG | - | chr1.3:59007235-59007254 | None:intergenic | 25.0% |
| ! | ATAATCGCCATACAATTAAT+CGG | - | chr1.3:59007444-59007463 | None:intergenic | 25.0% |
| ! | TTTAGTCCTAAGATCTTAAT+AGG | - | chr1.3:59006426-59006445 | None:intergenic | 25.0% |
| !! | AACACAATTTTTCTATGACT+TGG | - | chr1.3:59007038-59007057 | None:intergenic | 25.0% |
| !! | ACAGATTTTCATATACTTGT+GGG | + | chr1.3:59006449-59006468 | MS.gene44137:intron | 25.0% |
| !! | ATAAACACCGATTAATTGTA+TGG | + | chr1.3:59007434-59007453 | MS.gene44137:intron | 25.0% |
| !! | TCAAGTGAACAATTAATTTG+AGG | + | chr1.3:59006733-59006752 | MS.gene44137:intron | 25.0% |
| !! | TCAAGTTAGAATCTATTTTG+GGG | + | chr1.3:59006799-59006818 | MS.gene44137:intron | 25.0% |
| !! | TCTGAGATTTGGAAATAAAA+AGG | - | chr1.3:59007249-59007268 | None:intergenic | 25.0% |
| !! | TTTCTTTTTCTTATTGTCCT+TGG | + | chr1.3:59007394-59007413 | MS.gene44137:intron | 25.0% |
| !!! | CTTCAAGTTAGAATCTATTT+TGG | + | chr1.3:59006797-59006816 | MS.gene44137:intron | 25.0% |
| !!! | TTTTATTTCCAAATCTCAGA+TGG | + | chr1.3:59007249-59007268 | MS.gene44137:intron | 25.0% |
| AGAAGTTCACTATTCAATTG+AGG | - | chr1.3:59007492-59007511 | None:intergenic | 30.0% | |
| ATGGAAATTTACCAACAGTA+CGG | - | chr1.3:59005987-59006006 | None:intergenic | 30.0% | |
| TAAAAAGGATACACTTCAAG+GGG | - | chr1.3:59007234-59007253 | None:intergenic | 30.0% | |
| TCATGGCAAAATGAAAGTAA+GGG | - | chr1.3:59007201-59007220 | None:intergenic | 30.0% | |
| TTCATGGCAAAATGAAAGTA+AGG | - | chr1.3:59007202-59007221 | None:intergenic | 30.0% | |
| TTTCATATAACAAAGAGCCA+AGG | - | chr1.3:59007414-59007433 | None:intergenic | 30.0% | |
| ! | CAATTGATGTTCATTTTCAC+GGG | + | chr1.3:59007358-59007377 | MS.gene44137:intron | 30.0% |
| ! | GACAGATTTTCATATACTTG+TGG | + | chr1.3:59006448-59006467 | MS.gene44137:intron | 30.0% |
| ! | GCAATTGATGTTCATTTTCA+CGG | + | chr1.3:59007357-59007376 | MS.gene44137:intron | 30.0% |
| ! | GGGTAAGAAATTCTTCTTTT+TGG | + | chr1.3:59006966-59006985 | MS.gene44137:intron | 30.0% |
| ! | TGTTTGATTATTTTCGTTGC+AGG | + | chr1.3:59006295-59006314 | MS.gene44137:intron | 30.0% |
| !!! | TTAACACTGGTTTTCATGTT+AGG | - | chr1.3:59006159-59006178 | None:intergenic | 30.0% |
| AAAGTGAAAACTACCGCAAA+AGG | - | chr1.3:59006627-59006646 | None:intergenic | 35.0% | |
| AAATCGAATCTCTCAGGAAA+TGG | + | chr1.3:59005944-59005963 | MS.gene44137:CDS | 35.0% | |
| AATCGAATCTCTCAGGAAAT+GGG | + | chr1.3:59005945-59005964 | MS.gene44137:CDS | 35.0% | |
| ATTTCCTGAGAGATTCGATT+TGG | - | chr1.3:59005945-59005964 | None:intergenic | 35.0% | |
| CAGAAATTTCGTTGCATGAA+GGG | + | chr1.3:59006272-59006291 | MS.gene44137:intron | 35.0% | |
| GAAAAATTGTGTTTCGCAAC+AGG | + | chr1.3:59007045-59007064 | MS.gene44137:intron | 35.0% | |
| GTCTCTCCTATTAAGATCTT+AGG | + | chr1.3:59006417-59006436 | MS.gene44137:intron | 35.0% | |
| TCAGAAATTTCGTTGCATGA+AGG | + | chr1.3:59006271-59006290 | MS.gene44137:intron | 35.0% | |
| TGATCACAAGTCTGAAGAAA+AGG | + | chr1.3:59007671-59007690 | MS.gene44137:CDS | 35.0% | |
| TTTAACGAAGCAAGTCATCA+TGG | - | chr1.3:59006603-59006622 | None:intergenic | 35.0% | |
| TTTCCTGAGAGATTCGATTT+GGG | - | chr1.3:59005944-59005963 | None:intergenic | 35.0% | |
| ! | TGCTTCGTTAAATCCTTTTG+CGG | + | chr1.3:59006611-59006630 | MS.gene44137:intron | 35.0% |
| ! | TGTTGTTTGATTCTAGTGCA+TGG | + | chr1.3:59007289-59007308 | MS.gene44137:intron | 35.0% |
| ! | TTTCTCGATTAGGTTGTTTG+TGG | + | chr1.3:59006091-59006110 | MS.gene44137:intron | 35.0% |
| ! | TTTTGACGAGGGTTACTATA+GGG | + | chr1.3:59006564-59006583 | MS.gene44137:intron | 35.0% |
| !! | AGATTGTGAAGTTTTGACGA+GGG | + | chr1.3:59006553-59006572 | MS.gene44137:intron | 35.0% |
| !! | AGTTTTCACTTTTGCCTGAT+TGG | + | chr1.3:59006635-59006654 | MS.gene44137:intron | 35.0% |
| !!! | CCTTTTTAGAGTCCTCAATT+GGG | + | chr1.3:59006933-59006952 | MS.gene44137:intron | 35.0% |
| !!! | CTTTTTAGAGTCCTCAATTG+GGG | + | chr1.3:59006934-59006953 | MS.gene44137:intron | 35.0% |
| !!! | TCCTTTTTAGAGTCCTCAAT+TGG | + | chr1.3:59006932-59006951 | MS.gene44137:intron | 35.0% |
| !!! | TGCGTTGTAGAAGCTTTTTT+TGG | + | chr1.3:59006996-59007015 | MS.gene44137:intron | 35.0% |
| AAACCCAAATCGAATCTCTC+AGG | + | chr1.3:59005938-59005957 | MS.gene44137:CDS | 40.0% | |
| ATGAAAGTAAGGGAGATGTG+AGG | - | chr1.3:59007191-59007210 | None:intergenic | 40.0% | |
| CAAGGGGAAACTTGAATTCA+TGG | - | chr1.3:59007218-59007237 | None:intergenic | 40.0% | |
| CACTATTCAATTGAGGAGAC+AGG | - | chr1.3:59007485-59007504 | None:intergenic | 40.0% | |
| GCATGGCAAAACGAAAGTAA+GGG | - | chr1.3:59007148-59007167 | None:intergenic | 40.0% | |
| GGCGTGAATAATCTTAACAC+TGG | - | chr1.3:59006172-59006191 | None:intergenic | 40.0% | |
| GTGTTAAGATTATTCACGCC+AGG | + | chr1.3:59006172-59006191 | MS.gene44137:CDS | 40.0% | |
| TCCAAATCTCAGATGGCAAT+TGG | + | chr1.3:59007256-59007275 | MS.gene44137:intron | 40.0% | |
| TGCATGGCAAAACGAAAGTA+AGG | - | chr1.3:59007149-59007168 | None:intergenic | 40.0% | |
| TTTGTGGCTTAGCGGTATTA+CGG | + | chr1.3:59006107-59006126 | MS.gene44137:CDS | 40.0% | |
| ! | ATGTTCATTTTCACGGGTCT+TGG | + | chr1.3:59007364-59007383 | MS.gene44137:intron | 40.0% |
| ! | CAGGTTGAGCGAATAAATCA+AGG | - | chr1.3:59007110-59007129 | None:intergenic | 40.0% |
| ! | CCCAATTGAGGACTCTAAAA+AGG | - | chr1.3:59006936-59006955 | None:intergenic | 40.0% |
| ! | TCCAATTGCCATCTGAGATT+TGG | - | chr1.3:59007260-59007279 | None:intergenic | 40.0% |
| ! | TTAGGTTGTTTGTGGCTTAG+CGG | + | chr1.3:59006099-59006118 | MS.gene44137:intron | 40.0% |
| !! | GAGATTGTGAAGTTTTGACG+AGG | + | chr1.3:59006552-59006571 | MS.gene44137:intron | 40.0% |
| !! | GTTTTGACGAGGGTTACTAT+AGG | + | chr1.3:59006563-59006582 | MS.gene44137:intron | 40.0% |
| !! | TCACTTTTGCCTGATTGGTT+CGG | + | chr1.3:59006640-59006659 | MS.gene44137:intron | 40.0% |
| AAAAGGCTGCAAGGGATTCT+AGG | + | chr1.3:59007688-59007707 | MS.gene44137:CDS | 45.0% | |
| ACGAAAGTAAGGGAGATGCA+AGG | - | chr1.3:59007138-59007157 | None:intergenic | 45.0% | |
| AGGGAGATGCAAGGTATAAC+AGG | - | chr1.3:59007129-59007148 | None:intergenic | 45.0% | |
| AGTCTGAAGAAAAGGCTGCA+AGG | + | chr1.3:59007679-59007698 | MS.gene44137:CDS | 45.0% | |
| CATGCTCAAGCTCTTACACT+GGG | + | chr1.3:59007615-59007634 | MS.gene44137:CDS | 45.0% | |
| CGAGAGAAAACTCGAATGCA+TGG | - | chr1.3:59007165-59007184 | None:intergenic | 45.0% | |
| CGGGTTCAATTGCGTACAAT+TGG | + | chr1.3:59006127-59006146 | MS.gene44137:CDS | 45.0% | |
| CTCAATTGGGGTAGTTGGAA+GGG | + | chr1.3:59006946-59006965 | MS.gene44137:intron | 45.0% | |
| GAACACAAACTCCGTACTGT+TGG | + | chr1.3:59005973-59005992 | MS.gene44137:CDS | 45.0% | |
| GTCTGAAGAAAAGGCTGCAA+GGG | + | chr1.3:59007680-59007699 | MS.gene44137:CDS | 45.0% | |
| TGAAACAATGCTGACGTGCT+TGG | + | chr1.3:59007335-59007354 | MS.gene44137:intron | 45.0% | |
| TTGTGGCTTAGCGGTATTAC+GGG | + | chr1.3:59006108-59006127 | MS.gene44137:CDS | 45.0% | |
| !! | CACTTTTGCCTGATTGGTTC+GGG | + | chr1.3:59006641-59006660 | MS.gene44137:intron | 45.0% |
| !!! | ACTTTTGCCTGATTGGTTCG+GGG | + | chr1.3:59006642-59006661 | MS.gene44137:intron | 45.0% |
| CCTCAATTGGGGTAGTTGGA+AGG | + | chr1.3:59006945-59006964 | MS.gene44137:intron | 50.0% | |
| CCTTCCAACTACCCCAATTG+AGG | - | chr1.3:59006948-59006967 | None:intergenic | 50.0% | |
| GAGTCCTCAATTGGGGTAGT+TGG | + | chr1.3:59006941-59006960 | MS.gene44137:intron | 50.0% | |
| GCATGCTCAAGCTCTTACAC+TGG | + | chr1.3:59007614-59007633 | MS.gene44137:CDS | 50.0% | |
| !! | ATTGGTTCGGGGACGAGAAT+TGG | + | chr1.3:59006653-59006672 | MS.gene44137:intron | 50.0% |
| !! | CTTACACTGGGAGCATTAGC+TGG | + | chr1.3:59007627-59007646 | MS.gene44137:CDS | 50.0% |
| !! | TCGGGGACGAGAATTGGTTT+TGG | + | chr1.3:59006659-59006678 | MS.gene44137:intron | 50.0% |
| AGCATTAGCTGGAGCTGCAG+TGG | + | chr1.3:59007638-59007657 | MS.gene44137:CDS | 55.0% | |
| TTCTCGTCCCCGAACCAATC+AGG | - | chr1.3:59006652-59006671 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 59005925 | 59007716 | 59005925 | ID=MS.gene44137 |
| chr1.3 | mRNA | 59005925 | 59007716 | 59005925 | ID=MS.gene44137.t1;Parent=MS.gene44137 |
| chr1.3 | exon | 59005925 | 59005994 | 59005925 | ID=MS.gene44137.t1.exon1;Parent=MS.gene44137.t1 |
| chr1.3 | CDS | 59005925 | 59005994 | 59005925 | ID=cds.MS.gene44137.t1;Parent=MS.gene44137.t1 |
| chr1.3 | exon | 59006103 | 59006193 | 59006103 | ID=MS.gene44137.t1.exon2;Parent=MS.gene44137.t1 |
| chr1.3 | CDS | 59006103 | 59006193 | 59006103 | ID=cds.MS.gene44137.t1;Parent=MS.gene44137.t1 |
| chr1.3 | exon | 59007611 | 59007716 | 59007611 | ID=MS.gene44137.t1.exon3;Parent=MS.gene44137.t1 |
| chr1.3 | CDS | 59007611 | 59007716 | 59007611 | ID=cds.MS.gene44137.t1;Parent=MS.gene44137.t1 |
| Gene Sequence |
| Protein sequence |
