Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44587.t1 | XP_004488690.1 | 52.9 | 227 | 94 | 4 | 1 | 221 | 1 | 220 | 4.30E-56 | 228 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44587.t1 | P08688 | 43.1 | 225 | 115 | 5 | 6 | 221 | 7 | 227 | 4.1e-47 | 189.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44587.t1 | A0A1S2XHS6 | 52.9 | 227 | 94 | 4 | 1 | 221 | 1 | 220 | 3.1e-56 | 228.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061092 | MS.gene44587 | 0.818802 | 1.62E-52 | -1.69E-46 |
| MS.gene061094 | MS.gene44587 | 0.822418 | 2.39E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44587.t1 | MTR_1g066530 | 48.472 | 229 | 105 | 4 | 1 | 223 | 1 | 222 | 3.28e-69 | 211 |
| MS.gene44587.t1 | MTR_6g073540 | 45.690 | 232 | 112 | 4 | 1 | 221 | 1 | 229 | 3.16e-60 | 189 |
| MS.gene44587.t1 | MTR_6g048620 | 46.460 | 226 | 109 | 5 | 5 | 221 | 4 | 226 | 9.08e-58 | 182 |
| MS.gene44587.t1 | MTR_6g045077 | 45.089 | 224 | 107 | 5 | 6 | 221 | 5 | 220 | 4.65e-55 | 175 |
| MS.gene44587.t1 | MTR_6g048910 | 44.493 | 227 | 108 | 6 | 5 | 221 | 4 | 222 | 2.12e-54 | 174 |
| MS.gene44587.t1 | MTR_6g072470 | 47.761 | 201 | 93 | 3 | 1 | 190 | 1 | 200 | 2.57e-54 | 173 |
| MS.gene44587.t1 | MTR_0695s0030 | 41.850 | 227 | 120 | 4 | 6 | 224 | 65 | 287 | 3.74e-54 | 176 |
| MS.gene44587.t1 | MTR_0057s0100 | 41.518 | 224 | 119 | 3 | 6 | 221 | 7 | 226 | 1.04e-53 | 172 |
| MS.gene44587.t1 | MTR_0057s0170 | 41.071 | 224 | 120 | 3 | 6 | 221 | 7 | 226 | 1.47e-52 | 169 |
| MS.gene44587.t1 | MTR_8g038100 | 42.731 | 227 | 118 | 4 | 6 | 224 | 281 | 503 | 2.01e-52 | 177 |
| MS.gene44587.t1 | MTR_7g103990 | 44.248 | 226 | 111 | 8 | 6 | 221 | 7 | 227 | 3.60e-52 | 168 |
| MS.gene44587.t1 | MTR_8g038070 | 41.964 | 224 | 118 | 4 | 6 | 221 | 219 | 438 | 4.82e-52 | 174 |
| MS.gene44587.t1 | MTR_6g040290 | 39.381 | 226 | 124 | 4 | 6 | 221 | 7 | 229 | 1.65e-46 | 154 |
| MS.gene44587.t1 | MTR_7g012000 | 40.566 | 212 | 89 | 3 | 6 | 210 | 7 | 188 | 2.72e-45 | 150 |
| MS.gene44587.t1 | MTR_0807s0020 | 37.333 | 225 | 120 | 6 | 6 | 221 | 6 | 218 | 6.10e-38 | 131 |
| MS.gene44587.t1 | MTR_0216s0030 | 36.410 | 195 | 110 | 5 | 6 | 191 | 3 | 192 | 1.00e-35 | 125 |
| MS.gene44587.t1 | MTR_4g102470 | 40.571 | 175 | 87 | 4 | 6 | 178 | 7 | 166 | 5.07e-33 | 119 |
| MS.gene44587.t1 | MTR_4g102470 | 32.941 | 170 | 87 | 5 | 57 | 224 | 7 | 151 | 1.16e-13 | 67.8 |
| MS.gene44587.t1 | MTR_6g048810 | 42.105 | 152 | 79 | 4 | 72 | 221 | 156 | 300 | 2.03e-31 | 117 |
| MS.gene44587.t1 | MTR_6g048880 | 38.857 | 175 | 94 | 5 | 52 | 221 | 12 | 178 | 1.14e-30 | 111 |
| MS.gene44587.t1 | MTR_6g049040 | 40.800 | 125 | 53 | 2 | 1 | 119 | 1 | 110 | 1.14e-20 | 84.7 |
| MS.gene44587.t1 | MTR_6g049040 | 30.081 | 123 | 75 | 2 | 66 | 188 | 16 | 127 | 1.83e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAATACCAAATTGTTAAT+GGG | + | 43115:90102-90121 | MS.gene44587:CDS | 20.0% |
| !! | CACTCAATATTATATACAAT+TGG | - | 43115:90152-90171 | None:intergenic | 20.0% |
| ! | ATAGACTATGGTTTATATTC+AGG | + | 43115:90230-90249 | MS.gene44587:CDS | 25.0% |
| ! | CAGAAATACCAAATTGTTAA+TGG | + | 43115:90101-90120 | MS.gene44587:CDS | 25.0% |
| ! | CATTAACAATTTGGTATTTC+TGG | - | 43115:90103-90122 | None:intergenic | 25.0% |
| ! | CTAAATTGTCTTCATGAAAT+AGG | - | 43115:90373-90392 | None:intergenic | 25.0% |
| !! | TTTGAAAAAGGAATTGATTC+CGG | + | 43115:90476-90495 | MS.gene44587:CDS | 25.0% |
| AACACATCAGAACTATGAAT+TGG | - | 43115:90128-90147 | None:intergenic | 30.0% | |
| ATTACTTACAGGTCCTATTA+TGG | + | 43115:90262-90281 | MS.gene44587:CDS | 30.0% | |
| ATTCAATGTGCATAGATGAA+CGG | - | 43115:90028-90047 | None:intergenic | 30.0% | |
| GAATGTGCAAAAATAGACTA+TGG | + | 43115:90218-90237 | MS.gene44587:CDS | 30.0% | |
| TACTTTGCACGTATCAATAT+TGG | + | 43115:90545-90564 | MS.gene44587:CDS | 30.0% | |
| TGAAGACAATTTAGTCACTA+TGG | + | 43115:90379-90398 | MS.gene44587:CDS | 30.0% | |
| ! | AACGAAGCTTACATTTTCAA+AGG | + | 43115:90518-90537 | MS.gene44587:CDS | 30.0% |
| ! | AACGAGGCATTTATATTCTT+TGG | + | 43115:90191-90210 | MS.gene44587:CDS | 30.0% |
| !! | TATAAATGCCTCGTTGTTTT+CGG | - | 43115:90186-90205 | None:intergenic | 30.0% |
| AATAGGACCTGTAAGTAATG+TGG | - | 43115:90261-90280 | None:intergenic | 35.0% | |
| GAATTGGCCCATTAACAATT+TGG | - | 43115:90112-90131 | None:intergenic | 35.0% | |
| GTCAAATCCACTTTCAAACA+CGG | - | 43115:90318-90337 | None:intergenic | 35.0% | |
| GTCGGTACAGTATTTGAAAA+AGG | + | 43115:90464-90483 | MS.gene44587:CDS | 35.0% | |
| GTTACTTGTTCGTAGATGAA+AGG | + | 43115:90048-90067 | MS.gene44587:CDS | 35.0% | |
| ! | ACTGGTGACTATTTTGTCAA+TGG | + | 43115:90587-90606 | MS.gene44587:CDS | 35.0% |
| ! | CAATCGCGAATTTTCTTAAC+CGG | - | 43115:90431-90450 | None:intergenic | 35.0% |
| !! | AATATTGGCTCAGTTGCATT+TGG | + | 43115:90560-90579 | MS.gene44587:CDS | 35.0% |
| !! | ATTTGGTATTTCTGGAATCC+AGG | - | 43115:90095-90114 | None:intergenic | 35.0% |
| CGTAATGTGAGAAGCAAAAC+CGG | - | 43115:90498-90517 | None:intergenic | 40.0% | |
| CTTACAGGTCCTATTATGGT+CGG | + | 43115:90266-90285 | MS.gene44587:CDS | 40.0% | |
| GATTGTTTCCGTTGTTTGGT+CGG | + | 43115:90446-90465 | MS.gene44587:CDS | 40.0% | |
| GGAAAAGCCACATTACTTAC+AGG | + | 43115:90251-90270 | MS.gene44587:CDS | 40.0% | |
| GTAGTTGTGAATTATGCTCC+TGG | + | 43115:90074-90093 | MS.gene44587:CDS | 40.0% | |
| GTGGATTTGACACTGCAATA+AGG | + | 43115:90327-90346 | MS.gene44587:CDS | 40.0% | |
| TAGAGTTAATCCGTGATGAG+TGG | + | 43115:90615-90634 | MS.gene44587:CDS | 40.0% | |
| TAGGATAACATCTTTCCCAG+TGG | - | 43115:90354-90373 | None:intergenic | 40.0% | |
| TGTAATATGCTGTGAAGAGC+AGG | - | 43115:90641-90660 | None:intergenic | 40.0% | |
| ! | ATACTGTACCGACCAAACAA+CGG | - | 43115:90457-90476 | None:intergenic | 40.0% |
| AAGGGAACCGTGTTTGAAAG+TGG | + | 43115:90308-90327 | MS.gene44587:CDS | 45.0% | |
| ACACTGCAATAAGGTCCACT+GGG | + | 43115:90336-90355 | MS.gene44587:CDS | 45.0% | |
| ATCACTTCAAGCGTCTTCGT+GGG | + | 43115:90405-90424 | MS.gene44587:CDS | 45.0% | |
| CTTTGATGCCGAAAACAACG+AGG | + | 43115:90175-90194 | MS.gene44587:CDS | 45.0% | |
| GCCATGTTTCCTTGTCTCAA+GGG | + | 43115:90290-90309 | MS.gene44587:CDS | 45.0% | |
| GTGCATAGATGAACGGAATG+CGG | - | 43115:90021-90040 | None:intergenic | 45.0% | |
| TATCACTTCAAGCGTCTTCG+TGG | + | 43115:90404-90423 | MS.gene44587:CDS | 45.0% | |
| TCCCTTGAGACAAGGAAACA+TGG | - | 43115:90294-90313 | None:intergenic | 45.0% | |
| TCGCGATTGTTTCCGTTGTT+TGG | + | 43115:90442-90461 | MS.gene44587:CDS | 45.0% | |
| TGCCATGTTTCCTTGTCTCA+AGG | + | 43115:90289-90308 | MS.gene44587:CDS | 45.0% | |
| ! | ATTGGCTCAGTTGCATTTGG+TGG | + | 43115:90563-90582 | MS.gene44587:CDS | 45.0% |
| !! | AACATGGCACCGACCATAAT+AGG | - | 43115:90278-90297 | None:intergenic | 45.0% |
| !! | CTCGTTGTTTTCGGCATCAA+AGG | - | 43115:90177-90196 | None:intergenic | 45.0% |
| AACACGGTTCCCTTGAGACA+AGG | - | 43115:90302-90321 | None:intergenic | 50.0% | |
| GACACTGCAATAAGGTCCAC+TGG | + | 43115:90335-90354 | MS.gene44587:CDS | 50.0% | |
| ! | TCAGTTGCATTTGGTGGCAC+TGG | + | 43115:90569-90588 | MS.gene44587:CDS | 50.0% |
| GAAGAGCAGGCCACTCATCA+CGG | - | 43115:90628-90647 | None:intergenic | 55.0% | |
| CTTCAAGCGTCTTCGTGGGC+CGG | + | 43115:90409-90428 | MS.gene44587:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43115 | gene | 89996 | 90670 | 89996 | ID=MS.gene44587 |
| 43115 | mRNA | 89996 | 90670 | 89996 | ID=MS.gene44587.t1;Parent=MS.gene44587 |
| 43115 | exon | 89996 | 90670 | 89996 | ID=MS.gene44587.t1.exon1;Parent=MS.gene44587.t1 |
| 43115 | CDS | 89996 | 90670 | 89996 | ID=cds.MS.gene44587.t1;Parent=MS.gene44587.t1 |
| Gene Sequence |
| Protein sequence |
