Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45430.t1 | XP_013443967.1 | 95.5 | 242 | 11 | 0 | 72 | 313 | 39 | 280 | 7.40E-131 | 476.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45430.t1 | Q2JRH1 | 42.6 | 216 | 109 | 5 | 98 | 311 | 34 | 236 | 4.1e-37 | 156.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45430.t1 | A0A072TKF1 | 95.5 | 242 | 11 | 0 | 72 | 313 | 39 | 280 | 5.3e-131 | 476.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057121 | MS.gene45430 | 0.821213 | 4.55E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45430.t1 | MTR_8g009700 | 93.023 | 258 | 18 | 0 | 56 | 313 | 23 | 280 | 0.0 | 502 |
| MS.gene45430.t1 | MTR_8g009680 | 88.142 | 253 | 27 | 2 | 62 | 311 | 63 | 315 | 8.99e-165 | 461 |
| MS.gene45430.t1 | MTR_6g018680 | 39.796 | 196 | 109 | 3 | 116 | 310 | 479 | 666 | 1.93e-38 | 144 |
| MS.gene45430.t1 | MTR_8g009240 | 67.273 | 55 | 15 | 1 | 5 | 56 | 70 | 124 | 2.81e-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45430.t1 | AT5G17660 | 63.415 | 246 | 80 | 4 | 73 | 313 | 68 | 308 | 3.74e-114 | 332 |
Find 71 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAACTGCTTAAAAGAAAT+TGG | 0.087540 | 8.4:+78291012 | None:intergenic |
| CTCTGATCAATTTGGCCTTA+TGG | 0.174747 | 8.4:-78294881 | MS.gene45430:CDS |
| TCTGATCAATTTGGCCTTAT+GGG | 0.206934 | 8.4:-78294880 | MS.gene45430:CDS |
| GAACATTGCTCTGATCAATT+TGG | 0.249003 | 8.4:-78294889 | MS.gene45430:CDS |
| TTTCTCAGACAGTACTTTGC+TGG | 0.310858 | 8.4:+78294815 | None:intergenic |
| TTTGCAGTTAATTTCGATTT+CGG | 0.313636 | 8.4:-78290971 | MS.gene45430:intron |
| CCCAAATGATTTCTCGCTTT+AGG | 0.331633 | 8.4:+78291382 | None:intergenic |
| ACCTAAAGCGAGAAATCATT+TGG | 0.332484 | 8.4:-78291383 | MS.gene45430:CDS |
| TCAACTGCTTAAAAGAAATT+GGG | 0.339051 | 8.4:+78291013 | None:intergenic |
| AAAATTCCTCCGCAACTTAG+AGG | 0.340276 | 8.4:-78294765 | MS.gene45430:intron |
| TAGCCTTGACACTGATTTCA+AGG | 0.344509 | 8.4:-78294911 | MS.gene45430:CDS |
| GTGCATCAACTTCATCTAAC+TGG | 0.350365 | 8.4:+78290114 | None:intergenic |
| ATCATTTGGGATTGGAAATA+CGG | 0.352445 | 8.4:-78291369 | MS.gene45430:CDS |
| TAAGCAACCCTATGGGGATT+AGG | 0.353856 | 8.4:-78290034 | MS.gene45430:CDS |
| TATGTCATATTTCAGAATTT+GGG | 0.367238 | 8.4:-78293432 | MS.gene45430:intron |
| TGGTCAAGCGTGCTGAGTTA+TGG | 0.382252 | 8.4:-78291184 | MS.gene45430:CDS |
| TTGCCACTCATGGTAGATAT+TGG | 0.411854 | 8.4:-78291966 | MS.gene45430:CDS |
| ATTTGGGTCATGGCAGAATC+AGG | 0.419446 | 8.4:-78293416 | MS.gene45430:CDS |
| TTCTCAGACAGTACTTTGCT+GGG | 0.420636 | 8.4:+78294816 | None:intergenic |
| GGTCAAGCGTGCTGAGTTAT+GGG | 0.420860 | 8.4:-78291183 | MS.gene45430:CDS |
| CCTCAATCTCTCTTACAACT+TGG | 0.429111 | 8.4:-78293562 | MS.gene45430:intron |
| GGGATTGGAAATACGGCAAA+AGG | 0.432298 | 8.4:-78291362 | MS.gene45430:intron |
| AGCGAGAAATCATTTGGGAT+TGG | 0.464188 | 8.4:-78291377 | MS.gene45430:CDS |
| CCTAAAGCGAGAAATCATTT+GGG | 0.468102 | 8.4:-78291382 | MS.gene45430:CDS |
| ATGGTTGTTAAGCAACCCTA+TGG | 0.468931 | 8.4:-78290042 | MS.gene45430:CDS |
| TCTACCATGAGTGGCAATGC+AGG | 0.471708 | 8.4:+78291972 | None:intergenic |
| TTCCAGTCCGGTACTTGTGC+AGG | 0.475538 | 8.4:+78292011 | None:intergenic |
| TAATTTATCTCCCGGAGGAC+AGG | 0.477829 | 8.4:-78290531 | MS.gene45430:intron |
| GCAGCGGCAGGTTTCTTATG+TGG | 0.479775 | 8.4:-78291420 | MS.gene45430:CDS |
| TGTGAAAATAAGAACCCATA+AGG | 0.488209 | 8.4:+78294866 | None:intergenic |
| CTCTCAGTCCTAATCCCCAT+AGG | 0.490442 | 8.4:+78290026 | None:intergenic |
| TGAGTTATGGGTAAAAGATC+TGG | 0.497316 | 8.4:-78291171 | MS.gene45430:CDS |
| ATTGTAGATAATTTATCTCC+CGG | 0.499583 | 8.4:-78290539 | MS.gene45430:CDS |
| CAGAAAATATACCTGTCCTC+CGG | 0.501225 | 8.4:+78290520 | None:intergenic |
| AAATAATGTCAGGCAGCGGC+AGG | 0.509305 | 8.4:-78291432 | MS.gene45430:intron |
| AAGCTCACAACTTGTTGCAT+TGG | 0.510644 | 8.4:-78293595 | MS.gene45430:CDS |
| AGTTGTGAGCTTCGTAATTG+TGG | 0.514100 | 8.4:+78293606 | None:intergenic |
| CCAAGTTGTAAGAGAGATTG+AGG | 0.515832 | 8.4:+78293562 | None:intergenic |
| GAACATACAGAGAAAGAAGA+AGG | 0.534792 | 8.4:+78293381 | None:intergenic |
| GCAAAGACCTGGTTCCAGTC+CGG | 0.539469 | 8.4:+78291999 | None:intergenic |
| ATAGACTCCTGCACAAGTAC+CGG | 0.542196 | 8.4:-78292018 | MS.gene45430:intron |
| ACAAGTACCGGACTGGAACC+AGG | 0.546375 | 8.4:-78292006 | MS.gene45430:CDS |
| TCTCAGTCCTAATCCCCATA+GGG | 0.549156 | 8.4:+78290027 | None:intergenic |
| AGAAAATATACCTGTCCTCC+GGG | 0.550378 | 8.4:+78290521 | None:intergenic |
| GAGATTCATGCTGAATTCGA+AGG | 0.552662 | 8.4:-78290002 | MS.gene45430:CDS |
| ATGGGTAAAAGATCTGGCTC+TGG | 0.555239 | 8.4:-78291165 | MS.gene45430:CDS |
| GAAATTAACTGCAAAGGACC+AGG | 0.564124 | 8.4:+78290979 | None:intergenic |
| CTCCTGCACAAGTACCGGAC+TGG | 0.564496 | 8.4:-78292013 | MS.gene45430:CDS |
| TGGTTGTTAAGCAACCCTAT+GGG | 0.564909 | 8.4:-78290041 | MS.gene45430:CDS |
| AGACCCTGCATTGCCACTCA+TGG | 0.575995 | 8.4:-78291976 | MS.gene45430:CDS |
| AAGGATTGAAAATCTATCGA+AGG | 0.577412 | 8.4:-78289983 | MS.gene45430:CDS |
| CTCATGGTAGATATTGGATG+TGG | 0.587804 | 8.4:-78291960 | MS.gene45430:intron |
| ATGCAGGGTCTGCAAAGACC+TGG | 0.597840 | 8.4:+78291988 | None:intergenic |
| CAACTGCTTAAAAGAAATTG+GGG | 0.598384 | 8.4:+78291014 | None:intergenic |
| ATATTTCAGAATTTGGGTCA+TGG | 0.598590 | 8.4:-78293426 | MS.gene45430:intron |
| GTTCCTTGAAATCAGTGTCA+AGG | 0.600166 | 8.4:+78294908 | None:intergenic |
| CAGTTGATAGAATCATATCC+TGG | 0.600339 | 8.4:-78290997 | MS.gene45430:CDS |
| AAGGTTGTATGAGAAGAAGC+AGG | 0.607540 | 8.4:-78289964 | MS.gene45430:CDS |
| CTACCATGAGTGGCAATGCA+GGG | 0.613691 | 8.4:+78291973 | None:intergenic |
| GGCAATCTACCTCTAAGTTG+CGG | 0.630114 | 8.4:+78294756 | None:intergenic |
| GGTTGTTAAGCAACCCTATG+GGG | 0.644610 | 8.4:-78290040 | MS.gene45430:CDS |
| AATCTACCTCTAAGTTGCGG+AGG | 0.644985 | 8.4:+78294759 | None:intergenic |
| CAGAGTTCAAAGCATCTATG+TGG | 0.648244 | 8.4:+78290087 | None:intergenic |
| GTAGTGGCAGTAGCAGAACA+AGG | 0.649141 | 8.4:-78293642 | MS.gene45430:CDS |
| GAAATCGAAATTAACTGCAA+AGG | 0.655811 | 8.4:+78290973 | None:intergenic |
| CATCCAATATCTACCATGAG+TGG | 0.659846 | 8.4:+78291963 | None:intergenic |
| GATATATTGTGTGACAGTGA+CGG | 0.668648 | 8.4:-78290065 | MS.gene45430:CDS |
| TCTACAATAGTACCGACCAA+AGG | 0.679747 | 8.4:+78290554 | None:intergenic |
| ACAATCTGACGCACTTGATG+TGG | 0.682256 | 8.4:-78290153 | MS.gene45430:CDS |
| TATTGTGTGACAGTGACGGA+TGG | 0.692410 | 8.4:-78290061 | MS.gene45430:CDS |
| GTAGATAATTTATCTCCCGG+AGG | 0.720822 | 8.4:-78290536 | MS.gene45430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 78289959 | 78299048 | 78289959 | ID=MS.gene45430 |
| chr8.4 | mRNA | 78289959 | 78299048 | 78289959 | ID=MS.gene45430.t1;Parent=MS.gene45430 |
| chr8.4 | exon | 78299039 | 78299048 | 78299039 | ID=MS.gene45430.t1.exon1;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78299039 | 78299048 | 78299039 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78294766 | 78294930 | 78294766 | ID=MS.gene45430.t1.exon2;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78294766 | 78294930 | 78294766 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78293563 | 78293663 | 78293563 | ID=MS.gene45430.t1.exon3;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78293563 | 78293663 | 78293563 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78293389 | 78293439 | 78293389 | ID=MS.gene45430.t1.exon4;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78293389 | 78293439 | 78293389 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78291961 | 78292036 | 78291961 | ID=MS.gene45430.t1.exon5;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78291961 | 78292036 | 78291961 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78291363 | 78291442 | 78291363 | ID=MS.gene45430.t1.exon6;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78291363 | 78291442 | 78291363 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78291158 | 78291207 | 78291158 | ID=MS.gene45430.t1.exon7;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78291158 | 78291207 | 78291158 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78290972 | 78291056 | 78290972 | ID=MS.gene45430.t1.exon8;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78290972 | 78291056 | 78290972 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78290532 | 78290630 | 78290532 | ID=MS.gene45430.t1.exon9;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78290532 | 78290630 | 78290532 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| chr8.4 | exon | 78289959 | 78290183 | 78289959 | ID=MS.gene45430.t1.exon10;Parent=MS.gene45430.t1 |
| chr8.4 | CDS | 78289959 | 78290183 | 78289959 | ID=cds.MS.gene45430.t1;Parent=MS.gene45430.t1 |
| Gene Sequence |
| Protein sequence |
