Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45669.t1 | GAU39980.1 | 63.3 | 109 | 36 | 1 | 4 | 108 | 5 | 113 | 6.00E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45669.t1 | A0A2Z6NT15 | 63.3 | 109 | 36 | 1 | 4 | 108 | 5 | 113 | 4.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053294 | MS.gene45669 | 0.810353 | 1.20E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45669.t1 | MTR_2g010010 | 65.714 | 105 | 13 | 2 | 1 | 105 | 1 | 82 | 2.96e-38 | 124 |
| MS.gene45669.t1 | MTR_2g017540 | 41.441 | 111 | 52 | 1 | 14 | 124 | 25 | 122 | 3.26e-24 | 90.1 |
| MS.gene45669.t1 | MTR_1g070405 | 48.276 | 87 | 42 | 2 | 5 | 91 | 10 | 93 | 1.94e-22 | 85.5 |
| MS.gene45669.t1 | MTR_3g045410 | 50.685 | 73 | 34 | 1 | 15 | 87 | 54 | 124 | 4.65e-22 | 85.5 |
| MS.gene45669.t1 | MTR_5g021030 | 41.346 | 104 | 46 | 4 | 20 | 123 | 23 | 111 | 6.73e-22 | 84.0 |
| MS.gene45669.t1 | MTR_2g008290 | 41.346 | 104 | 46 | 4 | 20 | 123 | 23 | 111 | 8.92e-22 | 83.6 |
| MS.gene45669.t1 | MTR_1g039350 | 45.161 | 93 | 47 | 3 | 1 | 91 | 1 | 91 | 4.65e-21 | 81.6 |
| MS.gene45669.t1 | MTR_8g070590 | 54.412 | 68 | 29 | 1 | 20 | 87 | 22 | 87 | 4.90e-20 | 79.3 |
| MS.gene45669.t1 | MTR_5g046210 | 80.645 | 31 | 6 | 0 | 51 | 81 | 24 | 54 | 5.82e-13 | 59.3 |
| MS.gene45669.t1 | MTR_1g025050 | 69.231 | 39 | 10 | 2 | 20 | 58 | 24 | 60 | 4.59e-12 | 57.8 |
| MS.gene45669.t1 | MTR_3g117010 | 50.000 | 50 | 22 | 2 | 13 | 60 | 15 | 63 | 7.95e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGCTGTTGTGGTCATTA+TGG | 0.211304 | 4.4:-37785092 | MS.gene45669:CDS |
| AATCCTGGGCGCAGGTTTAA+TGG | 0.272546 | 4.4:-37785395 | MS.gene45669:CDS |
| CTAGCTGTTGTGGTCATTAT+GGG | 0.285906 | 4.4:-37785091 | MS.gene45669:CDS |
| ACGTGAAGCACAACTGTTAC+TGG | 0.310016 | 4.4:+37785481 | None:intergenic |
| AGGGATGCAGTTTCTTCAAT+TGG | 0.391710 | 4.4:-37785261 | MS.gene45669:CDS |
| GGTCTGTGTTGTTATGTTGA+AGG | 0.410752 | 4.4:-37785065 | MS.gene45669:CDS |
| GGGCGCAGGTTTAATGGATG+TGG | 0.417726 | 4.4:-37785389 | MS.gene45669:CDS |
| TGGTACGATGAAGAAATGTC+TGG | 0.428975 | 4.4:-37785241 | MS.gene45669:CDS |
| GTAGGAGAGATGTGCGTTGC+TGG | 0.445951 | 4.4:-37785460 | MS.gene45669:CDS |
| ATTCACCTCTTACTACTGCT+TGG | 0.466260 | 4.4:-37785427 | MS.gene45669:CDS |
| AGTTGATGAACTTAAGAAGA+AGG | 0.472690 | 4.4:-37785182 | MS.gene45669:CDS |
| GCTTGGACAACTGAGAATCC+TGG | 0.478913 | 4.4:-37785410 | MS.gene45669:CDS |
| GTTGCTTATTCTAGCTGTTG+TGG | 0.480453 | 4.4:-37785101 | MS.gene45669:CDS |
| TGTTGTGGTCATTATGGGAT+TGG | 0.484490 | 4.4:-37785086 | MS.gene45669:CDS |
| TAATGGATGTGGGTTATACA+AGG | 0.516891 | 4.4:-37785378 | MS.gene45669:intron |
| GTTGTCCAAGCAGTAGTAAG+AGG | 0.528710 | 4.4:+37785422 | None:intergenic |
| CAATCAGTTGCAGGGACGAA+AGG | 0.534522 | 4.4:-37785281 | MS.gene45669:intron |
| CATGAAGTTGAAAGAAGATG+AGG | 0.547179 | 4.4:-37785155 | MS.gene45669:CDS |
| AATCAGTTGCAGGGACGAAA+GGG | 0.548663 | 4.4:-37785280 | MS.gene45669:intron |
| CTTGGACAACTGAGAATCCT+GGG | 0.566427 | 4.4:-37785409 | MS.gene45669:CDS |
| GGCGCAGGTTTAATGGATGT+GGG | 0.572661 | 4.4:-37785388 | MS.gene45669:CDS |
| TGTCTGGGCAATCAGTTGCA+GGG | 0.577802 | 4.4:-37785289 | MS.gene45669:intron |
| CATCCATTAAACCTGCGCCC+AGG | 0.591823 | 4.4:+37785392 | None:intergenic |
| AATGTCTGGGCGTGCAAATG+AGG | 0.599624 | 4.4:-37785227 | MS.gene45669:CDS |
| CAACTGAGAATCCTGGGCGC+AGG | 0.600457 | 4.4:-37785403 | MS.gene45669:CDS |
| GGTACGATGAAGAAATGTCT+GGG | 0.616900 | 4.4:-37785240 | MS.gene45669:CDS |
| GTAACAGTTGTGCTTCACGT+AGG | 0.750736 | 4.4:-37785478 | MS.gene45669:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAACAAATTGTTAATTC+TGG | + | chr4.4:37785254-37785273 | None:intergenic | 20.0% |
| !! | CAAAACAAATTGTTAATTCT+GGG | + | chr4.4:37785253-37785272 | None:intergenic | 20.0% |
| !!! | AATTAACAATTTGTTTTGTC+TGG | - | chr4.4:37785255-37785274 | MS.gene45669:CDS | 20.0% |
| !!! | ATTAACAATTTGTTTTGTCT+GGG | - | chr4.4:37785256-37785275 | MS.gene45669:CDS | 20.0% |
| !! | AAGAAGCTAAAATTTTGTCA+AGG | - | chr4.4:37785436-37785455 | MS.gene45669:CDS | 25.0% |
| !! | AGAAGCTAAAATTTTGTCAA+GGG | - | chr4.4:37785437-37785456 | MS.gene45669:CDS | 25.0% |
| ! | TGAGGTGATTTTGTCATTAA+TGG | - | chr4.4:37785351-37785370 | MS.gene45669:intron | 30.0% |
| !! | AGTTGATGAACTTAAGAAGA+AGG | - | chr4.4:37785378-37785397 | MS.gene45669:intron | 30.0% |
| CATGAAGTTGAAAGAAGATG+AGG | - | chr4.4:37785405-37785424 | MS.gene45669:CDS | 35.0% | |
| ! | TAATGGATGTGGGTTATACA+AGG | - | chr4.4:37785182-37785201 | MS.gene45669:CDS | 35.0% |
| AGGGATGCAGTTTCTTCAAT+TGG | - | chr4.4:37785299-37785318 | MS.gene45669:intron | 40.0% | |
| ATTCACCTCTTACTACTGCT+TGG | - | chr4.4:37785133-37785152 | MS.gene45669:CDS | 40.0% | |
| CTAGCTGTTGTGGTCATTAT+GGG | - | chr4.4:37785469-37785488 | MS.gene45669:CDS | 40.0% | |
| GGTACGATGAAGAAATGTCT+GGG | - | chr4.4:37785320-37785339 | MS.gene45669:intron | 40.0% | |
| TCTAGCTGTTGTGGTCATTA+TGG | - | chr4.4:37785468-37785487 | MS.gene45669:CDS | 40.0% | |
| TGGTACGATGAAGAAATGTC+TGG | - | chr4.4:37785319-37785338 | MS.gene45669:intron | 40.0% | |
| TGTTGTGGTCATTATGGGAT+TGG | - | chr4.4:37785474-37785493 | MS.gene45669:CDS | 40.0% | |
| ! | GGTCTGTGTTGTTATGTTGA+AGG | - | chr4.4:37785495-37785514 | MS.gene45669:CDS | 40.0% |
| ! | GTTGCTTATTCTAGCTGTTG+TGG | - | chr4.4:37785459-37785478 | MS.gene45669:CDS | 40.0% |
| AATCAGTTGCAGGGACGAAA+GGG | - | chr4.4:37785280-37785299 | MS.gene45669:intron | 45.0% | |
| ACGTGAAGCACAACTGTTAC+TGG | + | chr4.4:37785082-37785101 | None:intergenic | 45.0% | |
| CTTGGACAACTGAGAATCCT+GGG | - | chr4.4:37785151-37785170 | MS.gene45669:CDS | 45.0% | |
| GTAACAGTTGTGCTTCACGT+AGG | - | chr4.4:37785082-37785101 | MS.gene45669:CDS | 45.0% | |
| GTTGTCCAAGCAGTAGTAAG+AGG | + | chr4.4:37785141-37785160 | None:intergenic | 45.0% | |
| AATCCTGGGCGCAGGTTTAA+TGG | - | chr4.4:37785165-37785184 | MS.gene45669:CDS | 50.0% | |
| AATGTCTGGGCGTGCAAATG+AGG | - | chr4.4:37785333-37785352 | MS.gene45669:intron | 50.0% | |
| CAATCAGTTGCAGGGACGAA+AGG | - | chr4.4:37785279-37785298 | MS.gene45669:intron | 50.0% | |
| GCTTGGACAACTGAGAATCC+TGG | - | chr4.4:37785150-37785169 | MS.gene45669:CDS | 50.0% | |
| TGTCTGGGCAATCAGTTGCA+GGG | - | chr4.4:37785271-37785290 | MS.gene45669:CDS | 50.0% | |
| TTGTCTGGGCAATCAGTTGC+AGG | - | chr4.4:37785270-37785289 | MS.gene45669:CDS | 50.0% | |
| ! | GGCGCAGGTTTAATGGATGT+GGG | - | chr4.4:37785172-37785191 | MS.gene45669:CDS | 50.0% |
| CATCCATTAAACCTGCGCCC+AGG | + | chr4.4:37785171-37785190 | None:intergenic | 55.0% | |
| GTAGGAGAGATGTGCGTTGC+TGG | - | chr4.4:37785100-37785119 | MS.gene45669:CDS | 55.0% | |
| ! | GGGCGCAGGTTTAATGGATG+TGG | - | chr4.4:37785171-37785190 | MS.gene45669:CDS | 55.0% |
| CAACTGAGAATCCTGGGCGC+AGG | - | chr4.4:37785157-37785176 | MS.gene45669:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 37785060 | 37785522 | 37785060 | ID=MS.gene45669 |
| chr4.4 | mRNA | 37785060 | 37785522 | 37785060 | ID=MS.gene45669.t1;Parent=MS.gene45669 |
| chr4.4 | exon | 37785379 | 37785522 | 37785379 | ID=MS.gene45669.t1.exon1;Parent=MS.gene45669.t1 |
| chr4.4 | CDS | 37785379 | 37785522 | 37785379 | ID=cds.MS.gene45669.t1;Parent=MS.gene45669.t1 |
| chr4.4 | exon | 37785060 | 37785296 | 37785060 | ID=MS.gene45669.t1.exon2;Parent=MS.gene45669.t1 |
| chr4.4 | CDS | 37785060 | 37785296 | 37785060 | ID=cds.MS.gene45669.t1;Parent=MS.gene45669.t1 |
| Gene Sequence |
| Protein sequence |
