Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46263.t1 | XP_003602049.1 | 90.6 | 170 | 13 | 2 | 1 | 168 | 1 | 169 | 1.30E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46263.t1 | G7LH76 | 90.6 | 170 | 13 | 2 | 1 | 168 | 1 | 169 | 9.6e-79 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050612 | MS.gene46263 | 0.801106 | 1.05E-48 | -1.69E-46 |
| MS.gene051317 | MS.gene46263 | 0.81255 | 4.01E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene46263 | 0.80866 | 2.78E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46263.t1 | MTR_3g088400 | 93.529 | 170 | 8 | 2 | 1 | 168 | 1 | 169 | 2.11e-95 | 273 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46263.t1 | AT1G77400 | 32.203 | 236 | 85 | 9 | 2 | 168 | 3 | 232 | 1.29e-15 | 72.0 |
Find 64 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGAATGAGGGAGATTC+CGG | 0.280355 | 3.1:+71283052 | None:intergenic |
| CATTTGAATGGAACTGATCC+TGG | 0.357724 | 3.1:+71283124 | None:intergenic |
| ACGGCCCCTTCTTGCGCAAC+CGG | 0.388573 | 3.1:-71282924 | MS.gene46263:CDS |
| CGCACTCCGGTTGCGCAAGA+AGG | 0.392092 | 3.1:+71282918 | None:intergenic |
| CAGGATCAGTTCCATTCAAA+TGG | 0.392591 | 3.1:-71283123 | MS.gene46263:CDS |
| GGTTTGATTTCCCATTTGAA+TGG | 0.399102 | 3.1:+71283112 | None:intergenic |
| TGATGATGGTGGTGAGGAAT+GGG | 0.403240 | 3.1:+71283082 | None:intergenic |
| TCCAGTTCTGATGTATAATC+AGG | 0.413146 | 3.1:+71282815 | None:intergenic |
| CTAAGTCCTTGTCGCCGTTC+CGG | 0.421562 | 3.1:-71282757 | MS.gene46263:CDS |
| CTCCGTAGCCGCTTCAGAAA+AGG | 0.423405 | 3.1:+71282869 | None:intergenic |
| TTCTGAAGCGGCTACGGAGC+AGG | 0.428600 | 3.1:-71282865 | MS.gene46263:CDS |
| CCGGAGTGCGTGACTTCGTC+AGG | 0.434551 | 3.1:-71282905 | MS.gene46263:CDS |
| TTGGTGATGCTGAATGGGCT+TGG | 0.438084 | 3.1:-71282688 | MS.gene46263:CDS |
| ACCTGATTATACATCAGAAC+TGG | 0.451790 | 3.1:-71282816 | MS.gene46263:CDS |
| GCTTGAATGAGGGAGATTCC+GGG | 0.452305 | 3.1:+71283053 | None:intergenic |
| ATGATGATGGTGGTGAGGAA+TGG | 0.456155 | 3.1:+71283081 | None:intergenic |
| TGGTGAGGAATGGGGAGTCC+TGG | 0.459210 | 3.1:+71283091 | None:intergenic |
| CTCCTTCCGGTCAAGTCCTA+AGG | 0.468786 | 3.1:-71282972 | MS.gene46263:CDS |
| ACCCATTGGTGATGCTGAAT+GGG | 0.470201 | 3.1:-71282693 | MS.gene46263:CDS |
| ATTCCCGTACACGCTCCTTC+CGG | 0.478158 | 3.1:-71282985 | MS.gene46263:CDS |
| TTCTGATGTATAATCAGGTT+CGG | 0.481587 | 3.1:+71282820 | None:intergenic |
| GATGCTGAATGGGCTTGGTT+CGG | 0.481588 | 3.1:-71282683 | MS.gene46263:CDS |
| GCACTCCGGTTGCGCAAGAA+GGG | 0.484553 | 3.1:+71282919 | None:intergenic |
| ACATCAGAACTGGAGACAAT+TGG | 0.489478 | 3.1:-71282806 | MS.gene46263:CDS |
| CATCTCTCAGAACCGACCTT+AGG | 0.491967 | 3.1:+71282956 | None:intergenic |
| GCCCATTCAGCATCACCAAT+GGG | 0.491981 | 3.1:+71282692 | None:intergenic |
| AACCCATTGGTGATGCTGAA+TGG | 0.502313 | 3.1:-71282694 | MS.gene46263:CDS |
| TTGACCGGAAGGAGCGTGTA+CGG | 0.502706 | 3.1:+71282981 | None:intergenic |
| TGGAGGTTTGAGCTTGAATG+AGG | 0.514311 | 3.1:+71283042 | None:intergenic |
| CTGAGAGATGGCGGTTTGAA+CGG | 0.517905 | 3.1:-71282943 | MS.gene46263:CDS |
| ACAGAAGACGACACGGTTGA+AGG | 0.525245 | 3.1:+71282731 | None:intergenic |
| GATGATTCATTCAAGAAGCC+AGG | 0.528926 | 3.1:-71283142 | MS.gene46263:CDS |
| TAGGAGCCGAGTTGTGGTGG+AGG | 0.536112 | 3.1:+71283025 | None:intergenic |
| AGGATCAGTTCCATTCAAAT+GGG | 0.539229 | 3.1:-71283122 | MS.gene46263:CDS |
| CTCCTACAAGTTATCTCCGG+TGG | 0.542586 | 3.1:-71283008 | MS.gene46263:CDS |
| GCGGCTACGGAGCAGGAAGA+AGG | 0.543315 | 3.1:-71282858 | MS.gene46263:CDS |
| TGACCGGAAGGAGCGTGTAC+GGG | 0.549214 | 3.1:+71282982 | None:intergenic |
| TCAGCATCACCAATGGGTTG+TGG | 0.553882 | 3.1:+71282698 | None:intergenic |
| CGGCTCCTACAAGTTATCTC+CGG | 0.555895 | 3.1:-71283011 | MS.gene46263:CDS |
| CTAAGGTCGGTTCTGAGAGA+TGG | 0.555995 | 3.1:-71282955 | MS.gene46263:CDS |
| TTGTAGGAGCCGAGTTGTGG+TGG | 0.561649 | 3.1:+71283022 | None:intergenic |
| AACTTGTAGGAGCCGAGTTG+TGG | 0.563307 | 3.1:+71283019 | None:intergenic |
| AACCGACCTTAGGACTTGAC+CGG | 0.564275 | 3.1:+71282966 | None:intergenic |
| ACGGTTGAAGGCCGCCGGAA+CGG | 0.564700 | 3.1:+71282743 | None:intergenic |
| AGCCCATTCAGCATCACCAA+TGG | 0.565303 | 3.1:+71282691 | None:intergenic |
| GGAGGTTTGAGCTTGAATGA+GGG | 0.589475 | 3.1:+71283043 | None:intergenic |
| TTCAAATGGGAAATCAAACC+AGG | 0.594700 | 3.1:-71283109 | MS.gene46263:CDS |
| CACTCCGGTTGCGCAAGAAG+GGG | 0.595751 | 3.1:+71282920 | None:intergenic |
| ATCCACCGGAGATAACTTGT+AGG | 0.597714 | 3.1:+71283006 | None:intergenic |
| GATGATGGTGGTGAGGAATG+GGG | 0.602221 | 3.1:+71283083 | None:intergenic |
| AGGCCGCCGGAACGGCGACA+AGG | 0.608271 | 3.1:+71282751 | None:intergenic |
| TTCCGGTCAAGTCCTAAGGT+CGG | 0.611011 | 3.1:-71282968 | MS.gene46263:CDS |
| CTCAAACCTCCACCACAACT+CGG | 0.613111 | 3.1:-71283031 | MS.gene46263:CDS |
| CCACCATCATCATCAAAACC+CGG | 0.614647 | 3.1:-71283071 | MS.gene46263:CDS |
| GAGCGTGTACGGGAATCCAC+CGG | 0.615955 | 3.1:+71282992 | None:intergenic |
| ACGACACGGTTGAAGGCCGC+CGG | 0.618792 | 3.1:+71282738 | None:intergenic |
| CCTGACGAAGTCACGCACTC+CGG | 0.625012 | 3.1:+71282905 | None:intergenic |
| GCGTCGTCACCACAACCCAT+TGG | 0.626471 | 3.1:-71282707 | MS.gene46263:CDS |
| AGTCCTTGTCGCCGTTCCGG+CGG | 0.638705 | 3.1:-71282754 | MS.gene46263:CDS |
| GACCTTAGGACTTGACCGGA+AGG | 0.639720 | 3.1:+71282970 | None:intergenic |
| AGGTCGGTTCTGAGAGATGG+CGG | 0.643746 | 3.1:-71282952 | MS.gene46263:CDS |
| AACTGGAGACAATTGGTCGG+TGG | 0.669577 | 3.1:-71282799 | MS.gene46263:CDS |
| CAGAACTGGAGACAATTGGT+CGG | 0.680092 | 3.1:-71282802 | MS.gene46263:CDS |
| CGACGCAACAGAAGACGACA+CGG | 0.727699 | 3.1:+71282724 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCTGATGTATAATCAGGTT+CGG | + | chr3.1:71283013-71283032 | None:intergenic | 30.0% | |
| ACCTGATTATACATCAGAAC+TGG | - | chr3.1:71283014-71283033 | MS.gene46263:CDS | 35.0% | |
| AGGATCAGTTCCATTCAAAT+GGG | - | chr3.1:71282708-71282727 | MS.gene46263:CDS | 35.0% | |
| TTCAAATGGGAAATCAAACC+AGG | - | chr3.1:71282721-71282740 | MS.gene46263:CDS | 35.0% | |
| ! | GGTTTGATTTCCCATTTGAA+TGG | + | chr3.1:71282721-71282740 | None:intergenic | 35.0% |
| ! | TCCAGTTCTGATGTATAATC+AGG | + | chr3.1:71283018-71283037 | None:intergenic | 35.0% |
| ACATCAGAACTGGAGACAAT+TGG | - | chr3.1:71283024-71283043 | MS.gene46263:CDS | 40.0% | |
| CAGGATCAGTTCCATTCAAA+TGG | - | chr3.1:71282707-71282726 | MS.gene46263:CDS | 40.0% | |
| CATTTGAATGGAACTGATCC+TGG | + | chr3.1:71282709-71282728 | None:intergenic | 40.0% | |
| GATGATTCATTCAAGAAGCC+AGG | - | chr3.1:71282688-71282707 | MS.gene46263:CDS | 40.0% | |
| !!! | ATTCCGGGTTTTGATGATGA+TGG | + | chr3.1:71282765-71282784 | None:intergenic | 40.0% |
| !!! | TTTTGATGATGATGGTGGTG+AGG | + | chr3.1:71282757-71282776 | None:intergenic | 40.0% |
| AGCTTGAATGAGGGAGATTC+CGG | + | chr3.1:71282781-71282800 | None:intergenic | 45.0% | |
| CAGAACTGGAGACAATTGGT+CGG | - | chr3.1:71283028-71283047 | MS.gene46263:CDS | 45.0% | |
| CCACCATCATCATCAAAACC+CGG | - | chr3.1:71282759-71282778 | MS.gene46263:CDS | 45.0% | |
| GGAGGTTTGAGCTTGAATGA+GGG | + | chr3.1:71282790-71282809 | None:intergenic | 45.0% | |
| TGGAGGTTTGAGCTTGAATG+AGG | + | chr3.1:71282791-71282810 | None:intergenic | 45.0% | |
| ! | ATCCACCGGAGATAACTTGT+AGG | + | chr3.1:71282827-71282846 | None:intergenic | 45.0% |
| !! | AACCCATTGGTGATGCTGAA+TGG | - | chr3.1:71283136-71283155 | MS.gene46263:CDS | 45.0% |
| !! | ACCCATTGGTGATGCTGAAT+GGG | - | chr3.1:71283137-71283156 | MS.gene46263:CDS | 45.0% |
| !! | ATGATGATGGTGGTGAGGAA+TGG | + | chr3.1:71282752-71282771 | None:intergenic | 45.0% |
| !! | TCTTCTCGCCTTTTCTGAAG+CGG | - | chr3.1:71282953-71282972 | MS.gene46263:CDS | 45.0% |
| !! | TGATGATGGTGGTGAGGAAT+GGG | + | chr3.1:71282751-71282770 | None:intergenic | 45.0% |
| AACCGACCTTAGGACTTGAC+CGG | + | chr3.1:71282867-71282886 | None:intergenic | 50.0% | |
| AACTGGAGACAATTGGTCGG+TGG | - | chr3.1:71283031-71283050 | MS.gene46263:CDS | 50.0% | |
| AACTTGTAGGAGCCGAGTTG+TGG | + | chr3.1:71282814-71282833 | None:intergenic | 50.0% | |
| ACAGAAGACGACACGGTTGA+AGG | + | chr3.1:71283102-71283121 | None:intergenic | 50.0% | |
| AGCCCATTCAGCATCACCAA+TGG | + | chr3.1:71283142-71283161 | None:intergenic | 50.0% | |
| CATCTCTCAGAACCGACCTT+AGG | + | chr3.1:71282877-71282896 | None:intergenic | 50.0% | |
| CGGCTCCTACAAGTTATCTC+CGG | - | chr3.1:71282819-71282838 | MS.gene46263:CDS | 50.0% | |
| CTCAAACCTCCACCACAACT+CGG | - | chr3.1:71282799-71282818 | MS.gene46263:CDS | 50.0% | |
| CTCCTACAAGTTATCTCCGG+TGG | - | chr3.1:71282822-71282841 | MS.gene46263:CDS | 50.0% | |
| GCCCATTCAGCATCACCAAT+GGG | + | chr3.1:71283141-71283160 | None:intergenic | 50.0% | |
| GCTTGAATGAGGGAGATTCC+GGG | + | chr3.1:71282780-71282799 | None:intergenic | 50.0% | |
| TTCCGGTCAAGTCCTAAGGT+CGG | - | chr3.1:71282862-71282881 | MS.gene46263:CDS | 50.0% | |
| ! | CTGAGAGATGGCGGTTTGAA+CGG | - | chr3.1:71282887-71282906 | MS.gene46263:CDS | 50.0% |
| ! | GATGCTGAATGGGCTTGGTT+CGG | - | chr3.1:71283147-71283166 | MS.gene46263:CDS | 50.0% |
| !! | CTAAGGTCGGTTCTGAGAGA+TGG | - | chr3.1:71282875-71282894 | MS.gene46263:CDS | 50.0% |
| !! | GATGATGGTGGTGAGGAATG+GGG | + | chr3.1:71282750-71282769 | None:intergenic | 50.0% |
| !! | TCAGCATCACCAATGGGTTG+TGG | + | chr3.1:71283135-71283154 | None:intergenic | 50.0% |
| !! | TTGGTGATGCTGAATGGGCT+TGG | - | chr3.1:71283142-71283161 | MS.gene46263:CDS | 50.0% |
| !!! | CCGGGTTTTGATGATGATGG+TGG | + | chr3.1:71282762-71282781 | None:intergenic | 50.0% |
| ATTCCCGTACACGCTCCTTC+CGG | - | chr3.1:71282845-71282864 | MS.gene46263:CDS | 55.0% | |
| CGACGCAACAGAAGACGACA+CGG | + | chr3.1:71283109-71283128 | None:intergenic | 55.0% | |
| CTAAGTCCTTGTCGCCGTTC+CGG | - | chr3.1:71283073-71283092 | MS.gene46263:CDS | 55.0% | |
| CTCCGTAGCCGCTTCAGAAA+AGG | + | chr3.1:71282964-71282983 | None:intergenic | 55.0% | |
| CTCCTTCCGGTCAAGTCCTA+AGG | - | chr3.1:71282858-71282877 | MS.gene46263:CDS | 55.0% | |
| GACCTTAGGACTTGACCGGA+AGG | + | chr3.1:71282863-71282882 | None:intergenic | 55.0% | |
| TTGTAGGAGCCGAGTTGTGG+TGG | + | chr3.1:71282811-71282830 | None:intergenic | 55.0% | |
| ! | TTGACCGGAAGGAGCGTGTA+CGG | + | chr3.1:71282852-71282871 | None:intergenic | 55.0% |
| !! | AGGTCGGTTCTGAGAGATGG+CGG | - | chr3.1:71282878-71282897 | MS.gene46263:CDS | 55.0% |
| !!! | CGCCTTTTCTGAAGCGGCTA+CGG | - | chr3.1:71282959-71282978 | MS.gene46263:CDS | 55.0% |
| CACTCCGGTTGCGCAAGAAG+GGG | + | chr3.1:71282913-71282932 | None:intergenic | 60.0% | |
| CCTGACGAAGTCACGCACTC+CGG | + | chr3.1:71282928-71282947 | None:intergenic | 60.0% | |
| GAGCGTGTACGGGAATCCAC+CGG | + | chr3.1:71282841-71282860 | None:intergenic | 60.0% | |
| GCACTCCGGTTGCGCAAGAA+GGG | + | chr3.1:71282914-71282933 | None:intergenic | 60.0% | |
| GCGTCGTCACCACAACCCAT+TGG | - | chr3.1:71283123-71283142 | MS.gene46263:CDS | 60.0% | |
| TAGGAGCCGAGTTGTGGTGG+AGG | + | chr3.1:71282808-71282827 | None:intergenic | 60.0% | |
| TGGTGAGGAATGGGGAGTCC+TGG | + | chr3.1:71282742-71282761 | None:intergenic | 60.0% | |
| ! | TGACCGGAAGGAGCGTGTAC+GGG | + | chr3.1:71282851-71282870 | None:intergenic | 60.0% |
| !! | TTCTGAAGCGGCTACGGAGC+AGG | - | chr3.1:71282965-71282984 | MS.gene46263:CDS | 60.0% |
| ACGACACGGTTGAAGGCCGC+CGG | + | chr3.1:71283095-71283114 | None:intergenic | 65.0% | |
| ACGGCCCCTTCTTGCGCAAC+CGG | - | chr3.1:71282906-71282925 | MS.gene46263:CDS | 65.0% | |
| ACGGTTGAAGGCCGCCGGAA+CGG | + | chr3.1:71283090-71283109 | None:intergenic | 65.0% | |
| AGTCCTTGTCGCCGTTCCGG+CGG | - | chr3.1:71283076-71283095 | MS.gene46263:CDS | 65.0% | |
| CCGGAGTGCGTGACTTCGTC+AGG | - | chr3.1:71282925-71282944 | MS.gene46263:CDS | 65.0% | |
| CGCACTCCGGTTGCGCAAGA+AGG | + | chr3.1:71282915-71282934 | None:intergenic | 65.0% | |
| GCGGCTACGGAGCAGGAAGA+AGG | - | chr3.1:71282972-71282991 | MS.gene46263:CDS | 65.0% | |
| AGGCCGCCGGAACGGCGACA+AGG | + | chr3.1:71283082-71283101 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 71282673 | 71283179 | 71282673 | ID=MS.gene46263 |
| chr3.1 | mRNA | 71282673 | 71283179 | 71282673 | ID=MS.gene46263.t1;Parent=MS.gene46263 |
| chr3.1 | exon | 71282673 | 71283179 | 71282673 | ID=MS.gene46263.t1.exon1;Parent=MS.gene46263.t1 |
| chr3.1 | CDS | 71282673 | 71283179 | 71282673 | ID=cds.MS.gene46263.t1;Parent=MS.gene46263.t1 |
| Gene Sequence |
| Protein sequence |
