Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47638.t1 | XP_003628751.1 | 100 | 119 | 0 | 0 | 1 | 119 | 1 | 119 | 4.60E-58 | 233.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47638.t1 | P93654 | 79.0 | 119 | 23 | 1 | 1 | 119 | 1 | 117 | 4.2e-44 | 178.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47638.t1 | G7LGX8 | 100.0 | 119 | 0 | 0 | 1 | 119 | 1 | 119 | 3.3e-58 | 233.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052302 | MS.gene47638 | -0.803319 | 3.69E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene47638 | 0.811856 | 5.68E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47638.t1 | MTR_8g066340 | 100.000 | 119 | 0 | 0 | 1 | 119 | 1 | 119 | 3.58e-84 | 247 |
| MS.gene47638.t1 | MTR_5g029650 | 84.874 | 119 | 12 | 1 | 1 | 119 | 1 | 113 | 3.68e-67 | 204 |
| MS.gene47638.t1 | MTR_7g084590 | 63.866 | 119 | 37 | 1 | 1 | 119 | 1 | 113 | 7.48e-44 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47638.t1 | AT5G46860 | 78.992 | 119 | 23 | 1 | 1 | 119 | 1 | 117 | 5.65e-61 | 188 |
| MS.gene47638.t1 | AT4G17730 | 75.410 | 122 | 27 | 1 | 1 | 119 | 1 | 122 | 1.10e-56 | 177 |
| MS.gene47638.t1 | AT4G17730 | 75.410 | 122 | 27 | 1 | 1 | 119 | 1 | 122 | 1.48e-56 | 177 |
| MS.gene47638.t1 | AT5G16830 | 62.698 | 126 | 39 | 2 | 1 | 119 | 1 | 125 | 1.34e-44 | 147 |
| MS.gene47638.t1 | AT1G32270 | 49.020 | 102 | 37 | 2 | 33 | 119 | 107 | 208 | 2.74e-18 | 79.3 |
| MS.gene47638.t1 | AT1G32270 | 72.727 | 55 | 15 | 0 | 65 | 119 | 154 | 208 | 1.29e-17 | 77.8 |
Find 36 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCTTGATCTGTTCTTAT+TGG | 0.226571 | 6.1:-69929587 | MS.gene47638:CDS |
| CGGAGCTCAGGGGTATCTTT+GGG | 0.233346 | 6.1:+69929801 | None:intergenic |
| ACGGAGCTCAGGGGTATCTT+TGG | 0.253295 | 6.1:+69929800 | None:intergenic |
| TCAGAGAATTATGAGCTTCC+AGG | 0.393794 | 6.1:-69929970 | None:intergenic |
| ATTGAAGCTGGCCGGCCGTT+CGG | 0.402656 | 6.1:-69929945 | MS.gene47638:CDS |
| CCGGCAGCCACCGCCTGCGT+TGG | 0.409779 | 6.1:+69929885 | None:intergenic |
| CACAAGACAAGACAACATAT+TGG | 0.419081 | 6.1:-69929768 | MS.gene47638:CDS |
| CGGCAGCCACCGCCTGCGTT+GGG | 0.437078 | 6.1:+69929886 | None:intergenic |
| GAAAGCAACGTCTTGGAAGC+AGG | 0.441607 | 6.1:-69929610 | MS.gene47638:CDS |
| GACGCGGCGGACCGATCAAC+GGG | 0.441894 | 6.1:-69929917 | MS.gene47638:CDS |
| GGAGCTCAGGGGTATCTTTG+GGG | 0.447352 | 6.1:+69929802 | None:intergenic |
| ACAAGACAAGACAACATATT+GGG | 0.455371 | 6.1:-69929767 | MS.gene47638:CDS |
| GCCGGCCAGCTTCAATATCC+TGG | 0.456879 | 6.1:+69929952 | None:intergenic |
| GGGTCTTGCTTCCCGTTGAT+CGG | 0.467196 | 6.1:+69929906 | None:intergenic |
| CCAACGCAGGCGGTGGCTGC+CGG | 0.483600 | 6.1:-69929885 | MS.gene47638:CDS |
| CGCGTCTCGAACCGAACGGC+CGG | 0.491628 | 6.1:+69929934 | None:intergenic |
| AATTTCAGAAAGCAACGTCT+TGG | 0.496272 | 6.1:-69929617 | MS.gene47638:CDS |
| GCAAGACCCAACGCAGGCGG+TGG | 0.505246 | 6.1:-69929892 | MS.gene47638:CDS |
| AGCTTCCAGGATATTGAAGC+TGG | 0.537286 | 6.1:-69929957 | MS.gene47638:CDS |
| CGGCCGTTCGGTTCGAGACG+CGG | 0.540976 | 6.1:-69929933 | MS.gene47638:CDS |
| CAGAGGCTCGTTAATACCCT+CGG | 0.542593 | 6.1:-69929828 | MS.gene47638:CDS |
| TCCAGGATATTGAAGCTGGC+CGG | 0.545584 | 6.1:-69929953 | MS.gene47638:CDS |
| AAGACAACATATTGGGCAGT+TGG | 0.551926 | 6.1:-69929760 | MS.gene47638:CDS |
| CTTCTTGCTTGCATTAACAT+CGG | 0.554008 | 6.1:+69929686 | None:intergenic |
| CCGCCGTTTCGACGTTTCAG+AGG | 0.556561 | 6.1:-69929845 | MS.gene47638:CDS |
| GAGCCTCTGAAACGTCGAAA+CGG | 0.561496 | 6.1:+69929842 | None:intergenic |
| AAAGCAACGTCTTGGAAGCA+GGG | 0.578886 | 6.1:-69929609 | MS.gene47638:CDS |
| AGACGCGGCGGACCGATCAA+CGG | 0.581740 | 6.1:-69929918 | MS.gene47638:CDS |
| ACATATTGGGCAGTTGGTGA+AGG | 0.585467 | 6.1:-69929754 | MS.gene47638:CDS |
| GAAGCAAGACCCAACGCAGG+CGG | 0.609845 | 6.1:-69929895 | MS.gene47638:CDS |
| TGCTTGCATTAACATCGGCA+TGG | 0.611045 | 6.1:+69929691 | None:intergenic |
| CCGCCGCGTCTCGAACCGAA+CGG | 0.635407 | 6.1:+69929930 | None:intergenic |
| CGGGAAGCAAGACCCAACGC+AGG | 0.643716 | 6.1:-69929898 | MS.gene47638:CDS |
| GAGCTCAGGGGTATCTTTGG+GGG | 0.654694 | 6.1:+69929803 | None:intergenic |
| CCTCTGAAACGTCGAAACGG+CGG | 0.706213 | 6.1:+69929845 | None:intergenic |
| CCGTTCGGTTCGAGACGCGG+CGG | 0.734218 | 6.1:-69929930 | MS.gene47638:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAGACAAGACAACATATT+GGG | - | chr6.1:69929777-69929796 | MS.gene47638:CDS | 30.0% | |
| AATTTCAGAAAGCAACGTCT+TGG | - | chr6.1:69929927-69929946 | MS.gene47638:CDS | 35.0% | |
| CACAAGACAAGACAACATAT+TGG | - | chr6.1:69929776-69929795 | MS.gene47638:CDS | 35.0% | |
| CTTCTTGCTTGCATTAACAT+CGG | + | chr6.1:69929861-69929880 | None:intergenic | 35.0% | |
| GAAGCTTGATCTGTTCTTAT+TGG | - | chr6.1:69929957-69929976 | MS.gene47638:CDS | 35.0% | |
| ! | TTGTCTTGTGAAGCTTTTCA+CGG | + | chr6.1:69929766-69929785 | None:intergenic | 35.0% |
| !! | GGTGTTGATCTGAAAAATTC+CGG | + | chr6.1:69929681-69929700 | None:intergenic | 35.0% |
| AAGACAACATATTGGGCAGT+TGG | - | chr6.1:69929784-69929803 | MS.gene47638:CDS | 40.0% | |
| AAAGCAACGTCTTGGAAGCA+GGG | - | chr6.1:69929935-69929954 | MS.gene47638:CDS | 45.0% | |
| AGCTTCCAGGATATTGAAGC+TGG | - | chr6.1:69929587-69929606 | MS.gene47638:CDS | 45.0% | |
| TGCTTGCATTAACATCGGCA+TGG | + | chr6.1:69929856-69929875 | None:intergenic | 45.0% | |
| !! | ACATATTGGGCAGTTGGTGA+AGG | - | chr6.1:69929790-69929809 | MS.gene47638:CDS | 45.0% |
| CAGAGGCTCGTTAATACCCT+CGG | - | chr6.1:69929716-69929735 | MS.gene47638:CDS | 50.0% | |
| GAAAGCAACGTCTTGGAAGC+AGG | - | chr6.1:69929934-69929953 | MS.gene47638:CDS | 50.0% | |
| TCCAGGATATTGAAGCTGGC+CGG | - | chr6.1:69929591-69929610 | MS.gene47638:CDS | 50.0% | |
| ! | AAGCTTTTCACGGAGCTCAG+GGG | + | chr6.1:69929756-69929775 | None:intergenic | 50.0% |
| ! | GAAGCTTTTCACGGAGCTCA+GGG | + | chr6.1:69929757-69929776 | None:intergenic | 50.0% |
| ! | TGAAGCTTTTCACGGAGCTC+AGG | + | chr6.1:69929758-69929777 | None:intergenic | 50.0% |
| !! | GAGCCTCTGAAACGTCGAAA+CGG | + | chr6.1:69929705-69929724 | None:intergenic | 50.0% |
| GGGTCTTGCTTCCCGTTGAT+CGG | + | chr6.1:69929641-69929660 | None:intergenic | 55.0% | |
| ! | GGTATCTTTGGGGGTTCCGA+GGG | + | chr6.1:69929735-69929754 | None:intergenic | 55.0% |
| !! | ACGGAGCTCAGGGGTATCTT+TGG | + | chr6.1:69929747-69929766 | None:intergenic | 55.0% |
| !! | CCTCTGAAACGTCGAAACGG+CGG | + | chr6.1:69929702-69929721 | None:intergenic | 55.0% |
| !! | CGGAGCTCAGGGGTATCTTT+GGG | + | chr6.1:69929746-69929765 | None:intergenic | 55.0% |
| !! | GAGCTCAGGGGTATCTTTGG+GGG | + | chr6.1:69929744-69929763 | None:intergenic | 55.0% |
| !! | GGAGCTCAGGGGTATCTTTG+GGG | + | chr6.1:69929745-69929764 | None:intergenic | 55.0% |
| ATTGAAGCTGGCCGGCCGTT+CGG | - | chr6.1:69929599-69929618 | MS.gene47638:CDS | 60.0% | |
| CCGCCGTTTCGACGTTTCAG+AGG | - | chr6.1:69929699-69929718 | MS.gene47638:CDS | 60.0% | |
| GAAGCAAGACCCAACGCAGG+CGG | - | chr6.1:69929649-69929668 | MS.gene47638:CDS | 60.0% | |
| GCCGGCCAGCTTCAATATCC+TGG | + | chr6.1:69929595-69929614 | None:intergenic | 60.0% | |
| ! | GGGTATCTTTGGGGGTTCCG+AGG | + | chr6.1:69929736-69929755 | None:intergenic | 60.0% |
| AGACGCGGCGGACCGATCAA+CGG | - | chr6.1:69929626-69929645 | MS.gene47638:CDS | 65.0% | |
| CGGGAAGCAAGACCCAACGC+AGG | - | chr6.1:69929646-69929665 | MS.gene47638:CDS | 65.0% | |
| CCGCCGCGTCTCGAACCGAA+CGG | + | chr6.1:69929617-69929636 | None:intergenic | 70.0% | |
| CGCGTCTCGAACCGAACGGC+CGG | + | chr6.1:69929613-69929632 | None:intergenic | 70.0% | |
| GACGCGGCGGACCGATCAAC+GGG | - | chr6.1:69929627-69929646 | MS.gene47638:CDS | 70.0% | |
| GCAAGACCCAACGCAGGCGG+TGG | - | chr6.1:69929652-69929671 | MS.gene47638:CDS | 70.0% | |
| ! | CCGTTCGGTTCGAGACGCGG+CGG | - | chr6.1:69929614-69929633 | MS.gene47638:CDS | 70.0% |
| ! | CGGCCGTTCGGTTCGAGACG+CGG | - | chr6.1:69929611-69929630 | MS.gene47638:CDS | 70.0% |
| CCAACGCAGGCGGTGGCTGC+CGG | - | chr6.1:69929659-69929678 | MS.gene47638:CDS | 75.0% | |
| CGGCAGCCACCGCCTGCGTT+GGG | + | chr6.1:69929661-69929680 | None:intergenic | 75.0% | |
| ! | CCGGCAGCCACCGCCTGCGT+TGG | + | chr6.1:69929662-69929681 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 69929584 | 69929982 | 69929584 | ID=MS.gene47638 |
| chr6.1 | mRNA | 69929584 | 69929982 | 69929584 | ID=MS.gene47638.t1;Parent=MS.gene47638 |
| chr6.1 | exon | 69929584 | 69929982 | 69929584 | ID=MS.gene47638.t1.exon1;Parent=MS.gene47638.t1 |
| chr6.1 | CDS | 69929584 | 69929982 | 69929584 | ID=cds.MS.gene47638.t1;Parent=MS.gene47638.t1 |
| Gene Sequence |
| Protein sequence |
