Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47880.t1 | XP_003629945.1 | 98.8 | 408 | 5 | 0 | 10 | 417 | 70 | 477 | 3.50E-221 | 777.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47880.t1 | Q93YZ7 | 79.2 | 408 | 85 | 0 | 10 | 417 | 81 | 488 | 3.3e-183 | 642.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47880.t1 | G7L8T1 | 98.8 | 408 | 5 | 0 | 10 | 417 | 70 | 477 | 2.5e-221 | 777.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053272 | MS.gene47880 | 0.802984 | 4.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47880.t1 | MTR_8g088600 | 98.533 | 409 | 6 | 0 | 10 | 418 | 70 | 478 | 0.0 | 815 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47880.t1 | AT2G31810 | 78.973 | 409 | 86 | 0 | 10 | 418 | 81 | 489 | 0.0 | 675 |
| MS.gene47880.t1 | AT2G31810 | 77.662 | 385 | 85 | 1 | 35 | 418 | 35 | 419 | 0.0 | 620 |
| MS.gene47880.t1 | AT2G31810 | 43.820 | 178 | 94 | 4 | 11 | 185 | 244 | 418 | 7.28e-37 | 139 |
| MS.gene47880.t1 | AT2G31810 | 73.594 | 409 | 86 | 1 | 10 | 418 | 81 | 467 | 0.0 | 615 |
| MS.gene47880.t1 | AT5G16290 | 71.744 | 407 | 113 | 1 | 12 | 418 | 73 | 477 | 0.0 | 608 |
| MS.gene47880.t1 | AT5G16290 | 43.931 | 173 | 93 | 3 | 11 | 180 | 303 | 474 | 6.76e-38 | 143 |
| MS.gene47880.t1 | AT5G16290 | 71.744 | 407 | 113 | 1 | 12 | 418 | 73 | 477 | 0.0 | 608 |
| MS.gene47880.t1 | AT5G16290 | 43.931 | 173 | 93 | 3 | 11 | 180 | 303 | 474 | 6.76e-38 | 143 |
Find 103 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGAAATTTCGGCAAGTT+TGG | 0.241618 | 4.4:-12492603 | MS.gene47880:CDS |
| TACATACCTCAGCACGGTTT+TGG | 0.261925 | 4.4:+12493744 | None:intergenic |
| CTGTGTATGTTACAGGTTAC+TGG | 0.276804 | 4.4:-12492651 | MS.gene47880:intron |
| TCGGCTTACAACTGTGGTTC+CGG | 0.280343 | 4.4:-12489778 | MS.gene47880:CDS |
| GGAGTTCTAAACATTGTTAC+AGG | 0.294864 | 4.4:-12489991 | MS.gene47880:CDS |
| TAGAAGGGGCTATAACATTC+AGG | 0.328690 | 4.4:-12489959 | MS.gene47880:intron |
| ATGCTCTTATGTCAGCTTAC+TGG | 0.331884 | 4.4:-12479385 | MS.gene47880:intron |
| TTGTTGTTTCTGGTACTGAT+AGG | 0.341983 | 4.4:-12494006 | MS.gene47880:CDS |
| ACAGCTATTAATGCTCTTGT+TGG | 0.343686 | 4.4:-12492338 | MS.gene47880:CDS |
| CAAACTTGCCGAAATTTCTC+TGG | 0.350301 | 4.4:+12492604 | None:intergenic |
| ACGTCGTGCAGCAGCATTTA+CGG | 0.355458 | 4.4:+12488786 | None:intergenic |
| GCAGCTGAATACCGCCAAAA+TGG | 0.358419 | 4.4:+12492386 | None:intergenic |
| TTACAGGAGTCTTTGCTAGA+AGG | 0.359381 | 4.4:-12489975 | MS.gene47880:CDS |
| GTTGCAGTCCAGAGAAATTT+CGG | 0.367538 | 4.4:-12492612 | MS.gene47880:CDS |
| TTGTTTCAGGTGGCACGAAC+TGG | 0.378592 | 4.4:-12478542 | MS.gene47880:intron |
| CGGCTTACAACTGTGGTTCC+GGG | 0.378969 | 4.4:-12489777 | MS.gene47880:CDS |
| TGGACTGCAACCATCTTCCC+TGG | 0.383234 | 4.4:+12492624 | None:intergenic |
| AGTTCGGAGACACACGATTT+CGG | 0.388982 | 4.4:-12494146 | MS.gene47880:CDS |
| TGGGTGCATCTGCTCCATTT+TGG | 0.409790 | 4.4:-12492400 | MS.gene47880:CDS |
| AAACTGTCTGAAGGCTCTAT+AGG | 0.416760 | 4.4:+12491188 | None:intergenic |
| ATTAAAGAAATAGCTAGAAC+TGG | 0.427203 | 4.4:-12492579 | MS.gene47880:CDS |
| CAGAGATTGTTAGAACCATA+TGG | 0.428012 | 4.4:-12479337 | MS.gene47880:CDS |
| GGAAGATTCCGTTACGATTG+AGG | 0.430766 | 4.4:-12493592 | MS.gene47880:intron |
| ATACTTAGATTGCATTAAGA+AGG | 0.430892 | 4.4:-12492430 | MS.gene47880:intron |
| TGCATTAAGAAGGGAAAAGA+TGG | 0.440203 | 4.4:-12492420 | MS.gene47880:CDS |
| ATTGGACTTGTCCGTGAGTC+GGG | 0.448310 | 4.4:-12478515 | MS.gene47880:CDS |
| ATGTCAGCTTACTGGAGATC+TGG | 0.451443 | 4.4:-12479377 | MS.gene47880:intron |
| TGTTGTTTCTGGTACTGATA+GGG | 0.451855 | 4.4:-12494005 | MS.gene47880:CDS |
| ATCAATTCTCGCTCAGCAAA+TGG | 0.465555 | 4.4:+12488823 | None:intergenic |
| GGAATGATTAACAGAATAGC+TGG | 0.467756 | 4.4:-12494103 | MS.gene47880:CDS |
| TACTGGGGACCCAGGGAAGA+TGG | 0.476333 | 4.4:-12492634 | MS.gene47880:CDS |
| CTGTTGGTTTGAATCAAGAC+AGG | 0.482756 | 4.4:-12494042 | MS.gene47880:CDS |
| AGAGTATCCACGGAGGTGCT+TGG | 0.490482 | 4.4:+12478484 | None:intergenic |
| GTGTTCGTTGGCGATGAGAG+TGG | 0.490996 | 4.4:-12494124 | MS.gene47880:CDS |
| AGCTAAGATCGTGGACATCT+CGG | 0.492079 | 4.4:-12493613 | MS.gene47880:CDS |
| ATGCTTGTGAATGATGCTCC+TGG | 0.493572 | 4.4:-12490012 | MS.gene47880:CDS |
| TTGGGAATACACAGATTAAG+TGG | 0.503166 | 4.4:-12493653 | MS.gene47880:intron |
| GTAACAATGTTTAGAACTCC+AGG | 0.504311 | 4.4:+12489994 | None:intergenic |
| CTCGAGGGTTATTGTGTGAT+CGG | 0.508506 | 4.4:+12488714 | None:intergenic |
| CAGACCTCACAAATGCCATA+TGG | 0.508814 | 4.4:+12479322 | None:intergenic |
| GTGGCACGAACTGGACGCAT+TGG | 0.512323 | 4.4:-12478533 | MS.gene47880:CDS |
| AGCTTTAGCCCTGAAAATGC+TGG | 0.513506 | 4.4:+12488747 | None:intergenic |
| TCAAGTACTTGATGCTCAGT+GGG | 0.515705 | 4.4:-12491156 | MS.gene47880:CDS |
| TGTGTATGTTACAGGTTACT+GGG | 0.517564 | 4.4:-12492650 | MS.gene47880:intron |
| AACATTGAGTCATTAGCTGT+TGG | 0.517994 | 4.4:-12494058 | MS.gene47880:CDS |
| CACACGATTTCGGTGTTCGT+TGG | 0.523203 | 4.4:-12494136 | MS.gene47880:CDS |
| ATCAATTCACGTTCTACTTG+AGG | 0.525082 | 4.4:+12493791 | None:intergenic |
| TACAGGAGTCTTTGCTAGAA+GGG | 0.525094 | 4.4:-12489974 | MS.gene47880:CDS |
| GCAGCAATTGAATAAGTTAG+TGG | 0.527818 | 4.4:-12489727 | MS.gene47880:CDS |
| GCTTTGAACGAGAAGGTGAA+GGG | 0.528204 | 4.4:+12494252 | None:intergenic |
| TGCTTTGAACGAGAAGGTGA+AGG | 0.529153 | 4.4:+12494251 | None:intergenic |
| ATCAAGTACTTGATGCTCAG+TGG | 0.532325 | 4.4:-12491157 | MS.gene47880:CDS |
| GGACTGCAACCATCTTCCCT+GGG | 0.533876 | 4.4:+12492625 | None:intergenic |
| ACACAAATAAATCAATCAGA+AGG | 0.540820 | 4.4:+12478457 | None:intergenic |
| CATTGGACTTGTCCGTGAGT+CGG | 0.548477 | 4.4:-12478516 | MS.gene47880:CDS |
| TACTGGAGATCTGGACAAGA+TGG | 0.548502 | 4.4:-12479368 | MS.gene47880:CDS |
| GTCCACGATCTTAGCTCTGA+AGG | 0.550488 | 4.4:+12493620 | None:intergenic |
| GCTTCGTATCCAGATCTTGA+GGG | 0.557075 | 4.4:-12492365 | MS.gene47880:CDS |
| TGCTTCGTATCCAGATCTTG+AGG | 0.559999 | 4.4:-12492366 | MS.gene47880:CDS |
| ATGGCAAGTGGGTTATATCC+CGG | 0.560667 | 4.4:+12488842 | None:intergenic |
| GAATACACAGATTAAGTGGT+TGG | 0.566164 | 4.4:-12493649 | MS.gene47880:intron |
| CTGATTGACTCATCTGTACC+CGG | 0.567540 | 4.4:+12489759 | None:intergenic |
| CTCGCTCAGCAAATGGCAAG+TGG | 0.570261 | 4.4:+12488830 | None:intergenic |
| ACTTGTCCGTGAGTCGGGTG+TGG | 0.571277 | 4.4:-12478510 | MS.gene47880:CDS |
| CAGAAGTTGAAGGAATCTCT+CGG | 0.572266 | 4.4:-12489797 | MS.gene47880:CDS |
| TACTTAGATTGCATTAAGAA+GGG | 0.573249 | 4.4:-12492429 | MS.gene47880:intron |
| TGGAGTCCACACCCGACTCA+CGG | 0.576058 | 4.4:+12478504 | None:intergenic |
| AGAAATAGCTAGAACTGGAA+AGG | 0.583246 | 4.4:-12492574 | MS.gene47880:intron |
| GCTGGAGTGTTTGCGAGAAG+AGG | 0.583816 | 4.4:-12494085 | MS.gene47880:CDS |
| AATACTTGCTTTGAACGAGA+AGG | 0.587781 | 4.4:+12494245 | None:intergenic |
| AGAAGGGAAGAGAGTATCCA+CGG | 0.588571 | 4.4:+12478474 | None:intergenic |
| TTACAGGTTACTGGGGACCC+AGG | 0.591748 | 4.4:-12492642 | MS.gene47880:intron |
| CACAAATAAATCAATCAGAA+GGG | 0.591929 | 4.4:+12478458 | None:intergenic |
| ACAGGAGTCTTTGCTAGAAG+GGG | 0.592633 | 4.4:-12489973 | MS.gene47880:CDS |
| AGATGAGTCAATCAGCAAGT+TGG | 0.595540 | 4.4:-12489751 | MS.gene47880:CDS |
| TACACAGATTAAGTGGTTGG+TGG | 0.600558 | 4.4:-12493646 | MS.gene47880:intron |
| TATTTCCAGAGTTTAGCTGT+AGG | 0.601110 | 4.4:-12489825 | MS.gene47880:intron |
| TACAGGTTACTGGGGACCCA+GGG | 0.618439 | 4.4:-12492641 | MS.gene47880:intron |
| GTAGGACATTCAGAAGTTGA+AGG | 0.623086 | 4.4:-12489807 | MS.gene47880:CDS |
| TGACAAAACTGATACGCCTG+TGG | 0.633286 | 4.4:-12492294 | MS.gene47880:CDS |
| CAAAACTGATACGCCTGTGG+GGG | 0.635833 | 4.4:-12492291 | MS.gene47880:intron |
| AGCAATGACCTCAATCGTAA+CGG | 0.635927 | 4.4:+12493584 | None:intergenic |
| TGTTGGTTTGAATCAAGACA+GGG | 0.639032 | 4.4:-12494041 | MS.gene47880:CDS |
| TAAGTTAGTGGAGCTGCATG+AGG | 0.641559 | 4.4:-12489715 | MS.gene47880:intron |
| AATCTCTCGGCTTACAACTG+TGG | 0.643062 | 4.4:-12489784 | MS.gene47880:CDS |
| GCATTAAGAAGGGAAAAGAT+GGG | 0.648061 | 4.4:-12492419 | MS.gene47880:CDS |
| TCGCTCAGCAAATGGCAAGT+GGG | 0.649088 | 4.4:+12488831 | None:intergenic |
| AGAACCATATGGCATTTGTG+AGG | 0.653037 | 4.4:-12479326 | MS.gene47880:intron |
| GTGTATGTTACAGGTTACTG+GGG | 0.653049 | 4.4:-12492649 | MS.gene47880:intron |
| CTTTGAACGAGAAGGTGAAG+GGG | 0.656113 | 4.4:+12494253 | None:intergenic |
| GACAAAACTGATACGCCTGT+GGG | 0.659876 | 4.4:-12492293 | MS.gene47880:CDS |
| TTGACTGTAAAACTGTCTGA+AGG | 0.661413 | 4.4:+12491179 | None:intergenic |
| CACCTTCAGAGCTAAGATCG+TGG | 0.670216 | 4.4:-12493622 | MS.gene47880:CDS |
| GGATAATGCCTTCAGACTAG+CGG | 0.672373 | 4.4:-12490057 | MS.gene47880:intron |
| ACAAAACTGATACGCCTGTG+GGG | 0.675365 | 4.4:-12492292 | MS.gene47880:CDS |
| ATCAGTACCAGAAACAACAA+TGG | 0.675754 | 4.4:+12494009 | None:intergenic |
| GTGGACTCCAAGCACCTCCG+TGG | 0.676678 | 4.4:-12478491 | MS.gene47880:CDS |
| AGATCCCCAAAACCGTGCTG+AGG | 0.677253 | 4.4:-12493750 | MS.gene47880:intron |
| TTTGAACGAGAAGGTGAAGG+GGG | 0.683750 | 4.4:+12494254 | None:intergenic |
| CAAGTACTTGATGCTCAGTG+GGG | 0.698945 | 4.4:-12491155 | MS.gene47880:CDS |
| TGTAAGTACATACCTCAGCA+CGG | 0.718597 | 4.4:+12493738 | None:intergenic |
| CGATCACACAATAACCCTCG+AGG | 0.724076 | 4.4:-12488713 | MS.gene47880:intron |
| AGGGAAGAGAGTATCCACGG+AGG | 0.789275 | 4.4:+12478477 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 12478472 | 12494284 | 12478472 | ID=MS.gene47880 |
| chr4.4 | mRNA | 12478472 | 12494284 | 12478472 | ID=MS.gene47880.t1;Parent=MS.gene47880 |
| chr4.4 | exon | 12494250 | 12494284 | 12494250 | ID=MS.gene47880.t1.exon1;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12494250 | 12494284 | 12494250 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12493955 | 12494168 | 12493955 | ID=MS.gene47880.t1.exon2;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12493955 | 12494168 | 12493955 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12493751 | 12493834 | 12493751 | ID=MS.gene47880.t1.exon3;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12493751 | 12493834 | 12493751 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12493593 | 12493661 | 12493593 | ID=MS.gene47880.t1.exon4;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12493593 | 12493661 | 12493593 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12492575 | 12492658 | 12492575 | ID=MS.gene47880.t1.exon5;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12492575 | 12492658 | 12492575 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12492292 | 12492444 | 12492292 | ID=MS.gene47880.t1.exon6;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12492292 | 12492444 | 12492292 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12491136 | 12491222 | 12491136 | ID=MS.gene47880.t1.exon7;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12491136 | 12491222 | 12491136 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12489960 | 12490064 | 12489960 | ID=MS.gene47880.t1.exon8;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12489960 | 12490064 | 12489960 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12489716 | 12489838 | 12489716 | ID=MS.gene47880.t1.exon9;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12489716 | 12489838 | 12489716 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12488714 | 12488866 | 12488714 | ID=MS.gene47880.t1.exon10;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12488714 | 12488866 | 12488714 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12479327 | 12479392 | 12479327 | ID=MS.gene47880.t1.exon11;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12479327 | 12479392 | 12479327 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| chr4.4 | exon | 12478472 | 12478555 | 12478472 | ID=MS.gene47880.t1.exon12;Parent=MS.gene47880.t1 |
| chr4.4 | CDS | 12478472 | 12478555 | 12478472 | ID=cds.MS.gene47880.t1;Parent=MS.gene47880.t1 |
| Gene Sequence |
| Protein sequence |
