Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48413.t1 | XP_003602912.1 | 99.3 | 135 | 1 | 0 | 1 | 135 | 383 | 517 | 1.60E-69 | 271.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48413.t1 | Q7Y030 | 78.4 | 134 | 29 | 0 | 1 | 134 | 384 | 517 | 4.9e-56 | 218.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48413.t1 | G7J538 | 99.3 | 135 | 1 | 0 | 1 | 135 | 383 | 517 | 1.1e-69 | 271.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055374 | MS.gene48413 | 0.814207 | 1.73E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48413.t1 | MTR_3g100310 | 99.259 | 135 | 1 | 0 | 1 | 135 | 383 | 517 | 7.66e-95 | 283 |
| MS.gene48413.t1 | MTR_5g032100 | 72.593 | 135 | 37 | 0 | 1 | 135 | 369 | 503 | 3.92e-69 | 216 |
| MS.gene48413.t1 | MTR_4g084570 | 35.714 | 140 | 84 | 3 | 1 | 135 | 411 | 549 | 2.53e-27 | 105 |
| MS.gene48413.t1 | MTR_3g005340 | 35.252 | 139 | 84 | 3 | 1 | 134 | 408 | 545 | 1.45e-25 | 100 |
| MS.gene48413.t1 | MTR_1g031590 | 34.286 | 140 | 86 | 3 | 1 | 135 | 410 | 548 | 2.29e-25 | 100 |
| MS.gene48413.t1 | MTR_3g099480 | 34.211 | 152 | 82 | 3 | 1 | 134 | 387 | 538 | 2.91e-23 | 94.4 |
| MS.gene48413.t1 | MTR_4g023030 | 40.299 | 134 | 77 | 2 | 3 | 133 | 483 | 616 | 1.47e-22 | 92.4 |
| MS.gene48413.t1 | MTR_3g026650 | 38.060 | 134 | 80 | 2 | 3 | 133 | 423 | 556 | 1.20e-20 | 86.7 |
| MS.gene48413.t1 | MTR_5g059260 | 35.211 | 142 | 83 | 4 | 1 | 133 | 418 | 559 | 1.93e-20 | 86.3 |
| MS.gene48413.t1 | MTR_3g086570 | 29.927 | 137 | 94 | 1 | 1 | 135 | 413 | 549 | 5.13e-19 | 82.0 |
| MS.gene48413.t1 | MTR_3g096020 | 33.775 | 151 | 82 | 3 | 1 | 133 | 436 | 586 | 6.79e-19 | 82.0 |
| MS.gene48413.t1 | MTR_5g064620 | 30.719 | 153 | 88 | 3 | 1 | 135 | 361 | 513 | 2.44e-18 | 80.1 |
| MS.gene48413.t1 | MTR_4g082310 | 32.867 | 143 | 86 | 3 | 1 | 133 | 347 | 489 | 8.86e-18 | 78.6 |
| MS.gene48413.t1 | MTR_5g042200 | 32.867 | 143 | 86 | 4 | 1 | 133 | 335 | 477 | 3.73e-17 | 76.6 |
| MS.gene48413.t1 | MTR_6g016015 | 32.394 | 142 | 86 | 4 | 1 | 132 | 366 | 507 | 6.96e-17 | 75.9 |
| MS.gene48413.t1 | MTR_4g125460 | 31.469 | 143 | 86 | 4 | 4 | 135 | 339 | 480 | 7.90e-17 | 75.9 |
| MS.gene48413.t1 | MTR_4g125460 | 31.469 | 143 | 86 | 4 | 4 | 135 | 478 | 619 | 9.02e-17 | 75.9 |
| MS.gene48413.t1 | MTR_2g016470 | 31.250 | 144 | 84 | 6 | 1 | 134 | 477 | 615 | 1.23e-16 | 75.5 |
| MS.gene48413.t1 | MTR_2g059350 | 33.099 | 142 | 85 | 4 | 1 | 132 | 366 | 507 | 1.45e-16 | 75.1 |
| MS.gene48413.t1 | MTR_4g110030 | 33.099 | 142 | 85 | 4 | 1 | 132 | 367 | 508 | 3.59e-16 | 73.9 |
| MS.gene48413.t1 | MTR_5g081690 | 31.034 | 145 | 87 | 5 | 1 | 134 | 369 | 511 | 9.59e-16 | 72.8 |
| MS.gene48413.t1 | MTR_4g132590 | 31.206 | 141 | 90 | 5 | 1 | 135 | 355 | 494 | 1.96e-15 | 72.0 |
| MS.gene48413.t1 | MTR_4g132590 | 31.206 | 141 | 90 | 5 | 1 | 135 | 454 | 593 | 2.01e-15 | 72.0 |
| MS.gene48413.t1 | MTR_5g099010 | 34.307 | 137 | 86 | 4 | 1 | 134 | 365 | 500 | 3.57e-15 | 71.2 |
| MS.gene48413.t1 | MTR_1g101990 | 32.143 | 140 | 89 | 5 | 1 | 135 | 142 | 280 | 1.78e-14 | 68.6 |
| MS.gene48413.t1 | MTR_4g019210 | 28.671 | 143 | 91 | 4 | 1 | 133 | 480 | 621 | 1.30e-13 | 66.6 |
| MS.gene48413.t1 | MTR_3g115220 | 32.353 | 136 | 88 | 4 | 1 | 133 | 358 | 492 | 1.68e-13 | 66.2 |
| MS.gene48413.t1 | MTR_5g012770 | 32.353 | 136 | 87 | 5 | 1 | 133 | 361 | 494 | 3.00e-13 | 65.5 |
| MS.gene48413.t1 | MTR_4g100810 | 31.618 | 136 | 88 | 5 | 1 | 133 | 356 | 489 | 4.82e-13 | 65.1 |
| MS.gene48413.t1 | MTR_1g102030 | 30.282 | 142 | 89 | 5 | 1 | 135 | 346 | 484 | 9.98e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48413.t1 | AT4G24530 | 78.358 | 134 | 29 | 0 | 1 | 134 | 384 | 517 | 6.61e-73 | 226 |
| MS.gene48413.t1 | AT5G65470 | 76.692 | 133 | 31 | 0 | 1 | 133 | 370 | 502 | 9.73e-72 | 223 |
| MS.gene48413.t1 | AT2G01480 | 37.410 | 139 | 81 | 3 | 1 | 134 | 415 | 552 | 4.26e-26 | 102 |
| MS.gene48413.t1 | AT1G14970 | 35.971 | 139 | 83 | 3 | 1 | 134 | 270 | 407 | 5.21e-26 | 101 |
| MS.gene48413.t1 | AT1G14970 | 35.971 | 139 | 83 | 3 | 1 | 134 | 296 | 433 | 5.95e-26 | 101 |
| MS.gene48413.t1 | AT1G35510 | 36.691 | 139 | 82 | 3 | 1 | 134 | 420 | 557 | 6.32e-26 | 102 |
| MS.gene48413.t1 | AT1G14970 | 35.971 | 139 | 83 | 3 | 1 | 134 | 416 | 553 | 1.05e-25 | 101 |
| MS.gene48413.t1 | AT2G44500 | 38.806 | 134 | 79 | 2 | 3 | 133 | 428 | 561 | 1.04e-21 | 90.1 |
| MS.gene48413.t1 | AT3G07900 | 34.328 | 134 | 85 | 2 | 3 | 133 | 439 | 572 | 3.61e-21 | 88.6 |
| MS.gene48413.t1 | AT1G38065 | 34.507 | 142 | 84 | 4 | 1 | 133 | 287 | 428 | 1.41e-20 | 86.7 |
| MS.gene48413.t1 | AT1G38065 | 34.507 | 142 | 84 | 4 | 1 | 133 | 297 | 438 | 1.51e-20 | 86.3 |
| MS.gene48413.t1 | AT1G38131 | 34.507 | 142 | 84 | 4 | 1 | 133 | 416 | 557 | 2.06e-20 | 86.3 |
| MS.gene48413.t1 | AT1G22460 | 32.237 | 152 | 85 | 3 | 1 | 134 | 407 | 558 | 1.89e-18 | 80.5 |
| MS.gene48413.t1 | AT5G63390 | 33.824 | 136 | 87 | 2 | 2 | 134 | 414 | 549 | 3.25e-18 | 80.1 |
| MS.gene48413.t1 | AT3G26370 | 29.197 | 137 | 95 | 1 | 1 | 135 | 421 | 557 | 4.78e-18 | 79.3 |
| MS.gene48413.t1 | AT5G64600 | 31.788 | 151 | 85 | 3 | 1 | 133 | 364 | 514 | 1.94e-17 | 77.8 |
| MS.gene48413.t1 | AT1G14020 | 31.469 | 143 | 88 | 3 | 1 | 133 | 341 | 483 | 4.84e-16 | 73.6 |
| MS.gene48413.t1 | AT5G35570 | 30.714 | 140 | 87 | 3 | 4 | 133 | 501 | 640 | 7.27e-16 | 73.2 |
| MS.gene48413.t1 | AT4G38390 | 32.847 | 137 | 90 | 2 | 1 | 135 | 371 | 507 | 1.12e-15 | 72.8 |
| MS.gene48413.t1 | AT1G76270 | 32.353 | 136 | 88 | 4 | 1 | 133 | 352 | 486 | 1.26e-15 | 72.4 |
| MS.gene48413.t1 | AT3G02250 | 33.099 | 142 | 85 | 4 | 1 | 132 | 357 | 498 | 1.46e-15 | 72.4 |
| MS.gene48413.t1 | AT2G01480 | 35.185 | 108 | 67 | 1 | 1 | 105 | 415 | 522 | 6.65e-15 | 70.5 |
| MS.gene48413.t1 | AT1G20550 | 33.824 | 136 | 86 | 4 | 1 | 133 | 355 | 489 | 1.24e-14 | 69.7 |
| MS.gene48413.t1 | AT5G15740 | 30.282 | 142 | 89 | 4 | 1 | 132 | 357 | 498 | 2.85e-14 | 68.6 |
| MS.gene48413.t1 | AT2G03280 | 32.867 | 143 | 85 | 5 | 1 | 132 | 338 | 480 | 4.22e-14 | 68.2 |
| MS.gene48413.t1 | AT1G29200 | 30.556 | 144 | 85 | 6 | 1 | 134 | 356 | 494 | 5.01e-14 | 67.8 |
| MS.gene48413.t1 | AT1G29200 | 30.556 | 144 | 85 | 6 | 1 | 134 | 356 | 494 | 5.01e-14 | 67.8 |
| MS.gene48413.t1 | AT2G03280 | 32.867 | 143 | 85 | 5 | 1 | 132 | 365 | 507 | 5.04e-14 | 67.8 |
| MS.gene48413.t1 | AT1G29200 | 29.577 | 142 | 89 | 5 | 1 | 134 | 282 | 420 | 5.14e-14 | 67.8 |
| MS.gene48413.t1 | AT1G29200 | 29.452 | 146 | 84 | 6 | 1 | 134 | 472 | 610 | 5.78e-14 | 67.8 |
| MS.gene48413.t1 | AT4G16650 | 33.577 | 137 | 86 | 5 | 1 | 134 | 370 | 504 | 7.03e-14 | 67.4 |
| MS.gene48413.t1 | AT4G16650 | 33.577 | 137 | 86 | 5 | 1 | 134 | 370 | 504 | 7.03e-14 | 67.4 |
| MS.gene48413.t1 | AT2G37980 | 30.769 | 143 | 88 | 5 | 1 | 133 | 474 | 615 | 9.07e-14 | 67.4 |
| MS.gene48413.t1 | AT1G04910 | 31.206 | 141 | 87 | 5 | 1 | 134 | 338 | 475 | 1.38e-13 | 66.6 |
| MS.gene48413.t1 | AT3G54100 | 27.972 | 143 | 92 | 4 | 1 | 133 | 470 | 611 | 2.53e-13 | 65.9 |
| MS.gene48413.t1 | AT1G62330 | 29.496 | 139 | 91 | 5 | 1 | 133 | 512 | 649 | 1.13e-12 | 63.9 |
| MS.gene48413.t1 | AT5G01100 | 27.972 | 143 | 90 | 3 | 4 | 133 | 466 | 608 | 9.59e-12 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACCAAACATGGCACTTT+TGG | 0.192496 | 3.2:-83552022 | MS.gene48413:CDS |
| CATCTTCATTTCGGCTTCTC+CGG | 0.300796 | 3.2:-83552245 | MS.gene48413:CDS |
| GAGACAATGGCCAAAGTTAA+TGG | 0.337935 | 3.2:-83552319 | MS.gene48413:CDS |
| GCACAGCGACATCTTCATTT+CGG | 0.365352 | 3.2:-83552254 | MS.gene48413:CDS |
| TTTCAGCGTGCGGTTCTTGA+TGG | 0.397111 | 3.2:-83551961 | MS.gene48413:CDS |
| CGATGCCTTTCCATTAACTT+TGG | 0.411811 | 3.2:+83552309 | None:intergenic |
| ATCTTCATTTCGGCTTCTCC+GGG | 0.439681 | 3.2:-83552244 | MS.gene48413:CDS |
| TGGCCCAAAAGTGCCATGTT+TGG | 0.467421 | 3.2:+83552018 | None:intergenic |
| AGGTTTATGGTGGAAAAGCT+AGG | 0.473316 | 3.2:-83552402 | MS.gene48413:CDS |
| TGCTCTTATTCTGAAATAGC+TGG | 0.476445 | 3.2:+83551998 | None:intergenic |
| CTACACATGCAATCAGGAGC+AGG | 0.479880 | 3.2:+83551877 | None:intergenic |
| TTATGGTGGAAAAGCTAGGT+TGG | 0.500381 | 3.2:-83552398 | MS.gene48413:CDS |
| TAAGCTCTACACATGCAATC+AGG | 0.507747 | 3.2:+83551871 | None:intergenic |
| GGGCAATATGCACAATGCTC+TGG | 0.515544 | 3.2:-83552224 | MS.gene48413:intron |
| GGGACAGATAAGATTAAGAA+AGG | 0.523765 | 3.2:-83551923 | MS.gene48413:CDS |
| AGAGCATTGTGCATATTGCC+CGG | 0.559884 | 3.2:+83552226 | None:intergenic |
| TGATGGACATAAGAACAGAC+AGG | 0.569668 | 3.2:-83551944 | MS.gene48413:CDS |
| TCTAGTCTCTGCAGAGACAA+TGG | 0.592187 | 3.2:-83552332 | MS.gene48413:CDS |
| TTCAGAATAAGAGCATAGGC+TGG | 0.602241 | 3.2:-83551990 | MS.gene48413:CDS |
| GAAGACTATCAGACCAAACA+TGG | 0.603497 | 3.2:-83552031 | MS.gene48413:CDS |
| CTATTTCAGAATAAGAGCAT+AGG | 0.610443 | 3.2:-83551994 | MS.gene48413:CDS |
| CTGGTCAGAATTTCAGCGTG+CGG | 0.613267 | 3.2:-83551971 | MS.gene48413:CDS |
| AATGGCCAAAGTTAATGGAA+AGG | 0.628911 | 3.2:-83552314 | MS.gene48413:CDS |
| TACACATGCAATCAGGAGCA+GGG | 0.643795 | 3.2:+83551878 | None:intergenic |
| GATGGACATAAGAACAGACA+GGG | 0.720717 | 3.2:-83551943 | MS.gene48413:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAGATGTTTGGTTTAAA+CGG | + | chr3.2:83552184-83552203 | None:intergenic | 25.0% |
| !! | CTTTTCTTATCTTCCATAAA+AGG | + | chr3.2:83551918-83551937 | None:intergenic | 25.0% |
| !! | TGGTTTAAACGGTAAATTTT+GGG | + | chr3.2:83552173-83552192 | None:intergenic | 25.0% |
| !! | TTGGTTTAAACGGTAAATTT+TGG | + | chr3.2:83552174-83552193 | None:intergenic | 25.0% |
| ! | CTATTTCAGAATAAGAGCAT+AGG | - | chr3.2:83552276-83552295 | MS.gene48413:CDS | 30.0% |
| ! | GGTTTAAACGGTAAATTTTG+GGG | + | chr3.2:83552172-83552191 | None:intergenic | 30.0% |
| !! | AAGCACGTTATTTCCTTTTA+TGG | - | chr3.2:83551902-83551921 | MS.gene48413:CDS | 30.0% |
| AATGGCCAAAGTTAATGGAA+AGG | - | chr3.2:83551956-83551975 | MS.gene48413:CDS | 35.0% | |
| CTGAAGATGCAAAAGATGTT+TGG | + | chr3.2:83552193-83552212 | None:intergenic | 35.0% | |
| GGGACAGATAAGATTAAGAA+AGG | - | chr3.2:83552347-83552366 | MS.gene48413:CDS | 35.0% | |
| ! | CAAACATCTTTTGCATCTTC+AGG | - | chr3.2:83552191-83552210 | MS.gene48413:intron | 35.0% |
| !! | TGCTCTTATTCTGAAATAGC+TGG | + | chr3.2:83552275-83552294 | None:intergenic | 35.0% |
| CGATGCCTTTCCATTAACTT+TGG | + | chr3.2:83551964-83551983 | None:intergenic | 40.0% | |
| GAAGACTATCAGACCAAACA+TGG | - | chr3.2:83552239-83552258 | MS.gene48413:CDS | 40.0% | |
| GAGACAATGGCCAAAGTTAA+TGG | - | chr3.2:83551951-83551970 | MS.gene48413:CDS | 40.0% | |
| GATGGACATAAGAACAGACA+GGG | - | chr3.2:83552327-83552346 | MS.gene48413:CDS | 40.0% | |
| TAAGCTCTACACATGCAATC+AGG | + | chr3.2:83552402-83552421 | None:intergenic | 40.0% | |
| TGATTGCATGTGTAGAGCTT+AGG | - | chr3.2:83552401-83552420 | MS.gene48413:CDS | 40.0% | |
| TGTATTGCTGCAAGACATGT+AGG | - | chr3.2:83552142-83552161 | MS.gene48413:intron | 40.0% | |
| ! | ACATCTTTTGCATCTTCAGG+TGG | - | chr3.2:83552194-83552213 | MS.gene48413:intron | 40.0% |
| ! | AGACCAAACATGGCACTTTT+GGG | - | chr3.2:83552249-83552268 | MS.gene48413:CDS | 40.0% |
| ! | TGATGGACATAAGAACAGAC+AGG | - | chr3.2:83552326-83552345 | MS.gene48413:CDS | 40.0% |
| ! | TTATGGTGGAAAAGCTAGGT+TGG | - | chr3.2:83551872-83551891 | MS.gene48413:CDS | 40.0% |
| ! | TTCAGAATAAGAGCATAGGC+TGG | - | chr3.2:83552280-83552299 | MS.gene48413:CDS | 40.0% |
| ATCTTCATTTCGGCTTCTCC+GGG | - | chr3.2:83552026-83552045 | MS.gene48413:CDS | 45.0% | |
| CATCTTCATTTCGGCTTCTC+CGG | - | chr3.2:83552025-83552044 | MS.gene48413:CDS | 45.0% | |
| GCACAGCGACATCTTCATTT+CGG | - | chr3.2:83552016-83552035 | MS.gene48413:CDS | 45.0% | |
| TACACATGCAATCAGGAGCA+GGG | + | chr3.2:83552395-83552414 | None:intergenic | 45.0% | |
| TCTAGTCTCTGCAGAGACAA+TGG | - | chr3.2:83551938-83551957 | MS.gene48413:CDS | 45.0% | |
| ! | CAGACCAAACATGGCACTTT+TGG | - | chr3.2:83552248-83552267 | MS.gene48413:CDS | 45.0% |
| !! | AGAGCATTGTGCATATTGCC+CGG | + | chr3.2:83552047-83552066 | None:intergenic | 45.0% |
| CTACACATGCAATCAGGAGC+AGG | + | chr3.2:83552396-83552415 | None:intergenic | 50.0% | |
| CTGGTCAGAATTTCAGCGTG+CGG | - | chr3.2:83552299-83552318 | MS.gene48413:CDS | 50.0% | |
| GGGCAATATGCACAATGCTC+TGG | - | chr3.2:83552046-83552065 | MS.gene48413:CDS | 50.0% | |
| TTTCAGCGTGCGGTTCTTGA+TGG | - | chr3.2:83552309-83552328 | MS.gene48413:CDS | 50.0% | |
| !! | TGGCCCAAAAGTGCCATGTT+TGG | + | chr3.2:83552255-83552274 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 83551870 | 83552422 | 83551870 | ID=MS.gene48413 |
| chr3.2 | mRNA | 83551870 | 83552422 | 83551870 | ID=MS.gene48413.t1;Parent=MS.gene48413 |
| chr3.2 | exon | 83552225 | 83552422 | 83552225 | ID=MS.gene48413.t1.exon1;Parent=MS.gene48413.t1 |
| chr3.2 | CDS | 83552225 | 83552422 | 83552225 | ID=cds.MS.gene48413.t1;Parent=MS.gene48413.t1 |
| chr3.2 | exon | 83551870 | 83552079 | 83551870 | ID=MS.gene48413.t1.exon2;Parent=MS.gene48413.t1 |
| chr3.2 | CDS | 83551870 | 83552079 | 83551870 | ID=cds.MS.gene48413.t1;Parent=MS.gene48413.t1 |
| Gene Sequence |
| Protein sequence |
