Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48459.t1 | XP_013455531.1 | 89.5 | 390 | 39 | 1 | 1 | 390 | 1 | 388 | 3.80E-193 | 684.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48459.t1 | Q9S7T8 | 54.3 | 387 | 174 | 3 | 5 | 388 | 4 | 390 | 5.0e-117 | 422.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48459.t1 | A0A072UIQ8 | 89.5 | 390 | 39 | 1 | 1 | 390 | 1 | 388 | 2.8e-193 | 684.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006610 | MS.gene48459 | PPI |
| MS.gene065945 | MS.gene48459 | PPI |
| MS.gene031697 | MS.gene48459 | PPI |
| MS.gene48459 | MS.gene045585 | PPI |
| MS.gene48459 | MS.gene065944 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48459.t1 | MTR_4g045707 | 89.487 | 390 | 39 | 1 | 1 | 390 | 1 | 388 | 0.0 | 704 |
| MS.gene48459.t1 | MTR_3g101010 | 72.308 | 390 | 107 | 1 | 1 | 390 | 1 | 389 | 0.0 | 546 |
| MS.gene48459.t1 | MTR_8g027395 | 69.091 | 385 | 115 | 1 | 6 | 390 | 17 | 397 | 0.0 | 530 |
| MS.gene48459.t1 | MTR_3g018740 | 68.052 | 385 | 120 | 2 | 5 | 388 | 4 | 386 | 0.0 | 519 |
| MS.gene48459.t1 | MTR_3g048000 | 66.841 | 383 | 106 | 3 | 7 | 388 | 28 | 390 | 0.0 | 511 |
| MS.gene48459.t1 | MTR_3g101030 | 65.915 | 399 | 114 | 6 | 11 | 390 | 34 | 429 | 4.48e-177 | 500 |
| MS.gene48459.t1 | MTR_4g088525 | 65.489 | 368 | 117 | 2 | 25 | 389 | 8 | 368 | 1.57e-169 | 478 |
| MS.gene48459.t1 | MTR_2g046030 | 64.010 | 389 | 129 | 4 | 5 | 388 | 4 | 386 | 3.01e-169 | 478 |
| MS.gene48459.t1 | MTR_8g461160 | 62.720 | 397 | 134 | 4 | 1 | 388 | 1 | 392 | 4.34e-169 | 478 |
| MS.gene48459.t1 | MTR_8g027420 | 63.636 | 385 | 125 | 3 | 6 | 390 | 6 | 375 | 3.16e-168 | 475 |
| MS.gene48459.t1 | MTR_7g451890 | 62.211 | 389 | 143 | 2 | 6 | 390 | 14 | 402 | 6.16e-168 | 476 |
| MS.gene48459.t1 | MTR_2g045980 | 63.753 | 389 | 130 | 4 | 5 | 388 | 4 | 386 | 3.28e-167 | 473 |
| MS.gene48459.t1 | MTR_7g050750 | 62.051 | 390 | 144 | 2 | 5 | 390 | 7 | 396 | 5.77e-167 | 473 |
| MS.gene48459.t1 | MTR_3g101180 | 60.553 | 398 | 147 | 3 | 1 | 388 | 1 | 398 | 7.56e-167 | 473 |
| MS.gene48459.t1 | MTR_7g047780 | 62.982 | 389 | 140 | 2 | 6 | 390 | 14 | 402 | 1.86e-166 | 472 |
| MS.gene48459.t1 | MTR_3g101130 | 59.596 | 396 | 151 | 4 | 1 | 392 | 1 | 391 | 2.05e-162 | 461 |
| MS.gene48459.t1 | MTR_7g050810 | 61.323 | 393 | 140 | 4 | 6 | 390 | 16 | 404 | 2.57e-161 | 459 |
| MS.gene48459.t1 | MTR_4g088535 | 62.565 | 382 | 123 | 4 | 4 | 385 | 5 | 366 | 2.14e-159 | 453 |
| MS.gene48459.t1 | MTR_3g101050 | 62.176 | 386 | 119 | 5 | 10 | 389 | 33 | 397 | 1.38e-153 | 439 |
| MS.gene48459.t1 | MTR_3g102920 | 59.897 | 389 | 116 | 5 | 4 | 388 | 56 | 408 | 8.58e-149 | 427 |
| MS.gene48459.t1 | MTR_3g015620 | 58.290 | 386 | 135 | 3 | 6 | 388 | 16 | 378 | 9.21e-140 | 403 |
| MS.gene48459.t1 | MTR_3g101200 | 54.220 | 391 | 150 | 6 | 1 | 388 | 26 | 390 | 1.01e-129 | 378 |
| MS.gene48459.t1 | MTR_3g100995 | 67.315 | 257 | 81 | 2 | 134 | 390 | 11 | 264 | 7.88e-122 | 353 |
| MS.gene48459.t1 | MTR_3g101190 | 51.639 | 366 | 114 | 3 | 25 | 390 | 10 | 312 | 2.55e-120 | 351 |
| MS.gene48459.t1 | MTR_3g018747 | 67.308 | 260 | 69 | 3 | 130 | 388 | 9 | 253 | 4.65e-115 | 336 |
| MS.gene48459.t1 | MTR_3g100010 | 55.769 | 312 | 90 | 2 | 31 | 309 | 42 | 338 | 2.59e-108 | 322 |
| MS.gene48459.t1 | MTR_7g050830 | 52.866 | 314 | 115 | 3 | 6 | 297 | 16 | 318 | 1.33e-104 | 312 |
| MS.gene48459.t1 | MTR_3g015760 | 60.967 | 269 | 99 | 4 | 122 | 388 | 15 | 279 | 8.13e-100 | 298 |
| MS.gene48459.t1 | MTR_3g111160 | 70.476 | 210 | 59 | 3 | 182 | 390 | 13 | 220 | 2.95e-92 | 276 |
| MS.gene48459.t1 | MTR_3g100520 | 62.613 | 222 | 68 | 3 | 171 | 390 | 1 | 209 | 2.01e-91 | 274 |
| MS.gene48459.t1 | MTR_3g114310 | 41.013 | 395 | 210 | 11 | 11 | 390 | 24 | 410 | 8.82e-88 | 272 |
| MS.gene48459.t1 | MTR_2g046410 | 65.700 | 207 | 63 | 3 | 187 | 388 | 144 | 347 | 6.75e-87 | 267 |
| MS.gene48459.t1 | MTR_3g099970 | 67.857 | 196 | 46 | 3 | 130 | 325 | 20 | 198 | 7.20e-87 | 262 |
| MS.gene48459.t1 | MTR_3g101110 | 53.975 | 239 | 80 | 3 | 130 | 364 | 68 | 280 | 2.06e-78 | 243 |
| MS.gene48459.t1 | MTR_3g101020 | 57.754 | 187 | 52 | 2 | 204 | 390 | 1 | 160 | 2.27e-65 | 206 |
| MS.gene48459.t1 | MTR_3g101150 | 66.242 | 157 | 49 | 3 | 236 | 388 | 1 | 157 | 8.97e-63 | 199 |
| MS.gene48459.t1 | MTR_3g111150 | 57.062 | 177 | 52 | 1 | 8 | 184 | 1 | 153 | 2.10e-59 | 190 |
| MS.gene48459.t1 | MTR_3g102870 | 59.091 | 154 | 63 | 0 | 193 | 346 | 1 | 154 | 6.54e-58 | 187 |
| MS.gene48459.t1 | MTR_3g100990 | 57.595 | 158 | 64 | 2 | 1 | 158 | 1 | 155 | 8.21e-48 | 170 |
| MS.gene48459.t1 | MTR_4g093550 | 64.286 | 84 | 30 | 0 | 6 | 89 | 6 | 89 | 1.62e-28 | 107 |
| MS.gene48459.t1 | MTR_3g101140 | 55.556 | 90 | 34 | 3 | 306 | 390 | 28 | 116 | 1.28e-20 | 87.0 |
| MS.gene48459.t1 | MTR_6g046890 | 57.143 | 84 | 33 | 1 | 14 | 97 | 7 | 87 | 3.72e-20 | 86.3 |
| MS.gene48459.t1 | MTR_3g101080 | 81.250 | 32 | 6 | 0 | 349 | 380 | 8 | 39 | 8.93e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48459.t1 | AT1G47710 | 54.264 | 387 | 174 | 3 | 5 | 388 | 4 | 390 | 4.26e-149 | 427 |
| MS.gene48459.t1 | AT1G47710 | 54.264 | 387 | 174 | 3 | 5 | 388 | 31 | 417 | 1.93e-148 | 427 |
| MS.gene48459.t1 | AT2G25240 | 48.214 | 392 | 179 | 8 | 6 | 387 | 5 | 382 | 2.96e-119 | 351 |
| MS.gene48459.t1 | AT3G45220 | 45.918 | 392 | 196 | 5 | 6 | 387 | 5 | 390 | 4.18e-116 | 343 |
| MS.gene48459.t1 | AT2G26390 | 46.582 | 395 | 185 | 8 | 6 | 387 | 5 | 386 | 6.35e-112 | 333 |
| MS.gene48459.t1 | AT2G14540 | 45.361 | 388 | 185 | 6 | 5 | 387 | 32 | 397 | 1.28e-100 | 305 |
| MS.gene48459.t1 | AT1G64030 | 43.544 | 395 | 194 | 6 | 5 | 392 | 4 | 376 | 4.52e-100 | 302 |
| MS.gene48459.t1 | AT1G62170 | 42.455 | 391 | 195 | 5 | 6 | 389 | 66 | 433 | 2.30e-99 | 302 |
| MS.gene48459.t1 | AT1G62170 | 39.151 | 424 | 195 | 6 | 6 | 389 | 66 | 466 | 4.83e-92 | 285 |
| MS.gene48459.t1 | AT2G35580 | 43.396 | 371 | 178 | 9 | 27 | 387 | 23 | 371 | 1.64e-89 | 275 |
| MS.gene48459.t1 | AT1G64010 | 50.459 | 218 | 84 | 5 | 171 | 387 | 1 | 195 | 4.56e-63 | 201 |
| MS.gene48459.t1 | AT1G64010 | 50.246 | 203 | 77 | 5 | 186 | 387 | 2 | 181 | 2.34e-56 | 183 |
| MS.gene48459.t1 | AT1G62160 | 43.069 | 202 | 87 | 4 | 193 | 389 | 42 | 220 | 2.15e-34 | 127 |
| MS.gene48459.t1 | AT1G63280 | 54.783 | 115 | 48 | 2 | 130 | 242 | 8 | 120 | 3.04e-34 | 123 |
| MS.gene48459.t1 | AT1G51330 | 51.351 | 111 | 51 | 2 | 129 | 236 | 30 | 140 | 2.47e-26 | 104 |
| MS.gene48459.t1 | AT1G64020 | 38.983 | 118 | 65 | 4 | 7 | 120 | 6 | 120 | 4.10e-18 | 80.1 |
Find 99 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAATGCACTGTACTTTAA+AGG | 0.110889 | 3.1:-80276282 | MS.gene48459:CDS |
| CCAAGGTTCAATGTCTCATT+TGG | 0.239058 | 3.1:-80275952 | MS.gene48459:CDS |
| CAGTGCCTTCTTCGTTTACT+TGG | 0.246075 | 3.1:+80275797 | None:intergenic |
| CTTGAGAAAGATTAAACTTA+TGG | 0.265790 | 3.1:+80275998 | None:intergenic |
| TCATCTTGGCCCTGTTTATA+AGG | 0.290119 | 3.1:+80276114 | None:intergenic |
| CAGGTGCTCAATCCTCTTGC+TGG | 0.292012 | 3.1:-80275637 | MS.gene48459:CDS |
| TCCCGGAGGTTTGACAAATA+TGG | 0.292326 | 3.1:-80275878 | MS.gene48459:CDS |
| TTACTTGGATGAAAGACTTT+TGG | 0.294262 | 3.1:+80275812 | None:intergenic |
| GTCAAACCTCCGGGAGAAAA+AGG | 0.295384 | 3.1:+80275886 | None:intergenic |
| AAAATCATAGTCTTTAGTTT+TGG | 0.306750 | 3.1:+80276233 | None:intergenic |
| GAGACAAATGGACTTATTAA+AGG | 0.310786 | 3.1:-80276351 | MS.gene48459:CDS |
| ACACATTACATACCTTGTTC+TGG | 0.335608 | 3.1:+80276748 | None:intergenic |
| CACTGTACTTTAAAGGACTA+TGG | 0.351189 | 3.1:-80276275 | MS.gene48459:CDS |
| ACTTTGTCTTCCTTATAAAC+AGG | 0.354807 | 3.1:-80276124 | MS.gene48459:CDS |
| CAAGGTTCAATGTCTCATTT+GGG | 0.379500 | 3.1:-80275951 | MS.gene48459:CDS |
| AGCAAAGAGGATCAATATAT+TGG | 0.387919 | 3.1:-80276168 | MS.gene48459:CDS |
| AACCCATGAATTGACTTCAT+TGG | 0.393781 | 3.1:+80276380 | None:intergenic |
| CACAATCCTCTTTGTTGGAC+AGG | 0.397313 | 3.1:-80275656 | MS.gene48459:CDS |
| CGAAAACACGATGTTGTTGT+TGG | 0.397648 | 3.1:+80277049 | None:intergenic |
| ACCGGCACAATCCTCTTTGT+TGG | 0.412739 | 3.1:-80275661 | MS.gene48459:CDS |
| GTCGAGAAGTTGTTGCTTTG+TGG | 0.421022 | 3.1:+80276974 | None:intergenic |
| CTCAGCTTTGGTTGAAAAGG+TGG | 0.438522 | 3.1:-80276043 | MS.gene48459:CDS |
| AATCTTTCTCAAGTCAAAGT+AGG | 0.444523 | 3.1:-80275988 | MS.gene48459:CDS |
| CTCTGTCGCCAATGGTGTGT+GGG | 0.444711 | 3.1:-80276853 | MS.gene48459:CDS |
| GAAATCAACGGAAGCCAACT+TGG | 0.444733 | 3.1:+80276770 | None:intergenic |
| CACCATATTTGTCAAACCTC+CGG | 0.448925 | 3.1:+80275876 | None:intergenic |
| TAGAGAATCGGCGGTCATCT+TGG | 0.453950 | 3.1:+80276100 | None:intergenic |
| TGACCAATGAAGTCAATTCA+TGG | 0.453988 | 3.1:-80276383 | MS.gene48459:CDS |
| CGAAGGGAGCAGAGTCTTTG+AGG | 0.457996 | 3.1:+80276889 | None:intergenic |
| CAACGGAAGCCAACTTGGCT+TGG | 0.461840 | 3.1:+80276775 | None:intergenic |
| ACTCTCAGCTTTGGTTGAAA+AGG | 0.467571 | 3.1:-80276046 | MS.gene48459:CDS |
| AAAACATGGACTCTCAGCTT+TGG | 0.469650 | 3.1:-80276055 | MS.gene48459:CDS |
| CTCAAAGACTCTGCTCCCTT+CGG | 0.476884 | 3.1:-80276888 | MS.gene48459:CDS |
| TGCATTTGCAAAGATGAGTC+TGG | 0.477055 | 3.1:+80276296 | None:intergenic |
| AAAGACTCTGCTCCCTTCGG+CGG | 0.478699 | 3.1:-80276885 | MS.gene48459:CDS |
| AAGACTCTGCTCCCTTCGGC+GGG | 0.479210 | 3.1:-80276884 | MS.gene48459:CDS |
| TCTCTTATTAGAAATAAGAA+TGG | 0.482752 | 3.1:+80275691 | None:intergenic |
| GATACTGAGAATAACTTGAA+TGG | 0.484768 | 3.1:+80277019 | None:intergenic |
| CCTCGCCTCTCTGTCGCCAA+TGG | 0.491467 | 3.1:-80276861 | MS.gene48459:CDS |
| CTCTTATTAGAAATAAGAAT+GGG | 0.494945 | 3.1:+80275692 | None:intergenic |
| TTGTGAGTGAAACGTTGGTT+TGG | 0.497834 | 3.1:+80277099 | None:intergenic |
| AATATGGTGGACTCTCTTGA+AGG | 0.499593 | 3.1:-80275862 | MS.gene48459:CDS |
| GAATAAAGTACATAGAGAAT+CGG | 0.509178 | 3.1:+80276088 | None:intergenic |
| GGAAACAAGGCGAGAGGCGA+CGG | 0.514177 | 3.1:+80276917 | None:intergenic |
| TCCTCTTTGCTGCTCATGAA+CGG | 0.518225 | 3.1:+80276180 | None:intergenic |
| AGGTGCTCAATCCTCTTGCT+GGG | 0.520120 | 3.1:-80275636 | MS.gene48459:CDS |
| GTTGATCGATGGATTTGAAA+CGG | 0.521172 | 3.1:+80276946 | None:intergenic |
| TTGCTCAACCCACACACCAT+TGG | 0.522041 | 3.1:+80276845 | None:intergenic |
| AGGAGTTATTCAACCAGGGT+TGG | 0.525626 | 3.1:-80276331 | MS.gene48459:CDS |
| TTCTGATTTCCAAGCCAAGT+TGG | 0.531191 | 3.1:-80276784 | MS.gene48459:CDS |
| ACCATATTTGTCAAACCTCC+GGG | 0.531907 | 3.1:+80275877 | None:intergenic |
| ATCATCGCTGCTGGCTCTGA+GGG | 0.534728 | 3.1:-80276999 | MS.gene48459:CDS |
| TCTCTGTCGCCAATGGTGTG+TGG | 0.539480 | 3.1:-80276854 | MS.gene48459:CDS |
| TTCATCCAAGTAAACGAAGA+AGG | 0.540994 | 3.1:-80275802 | MS.gene48459:CDS |
| TATCATCGCTGCTGGCTCTG+AGG | 0.548469 | 3.1:-80277000 | MS.gene48459:CDS |
| TTCCGTTGATTTCCAGAACA+AGG | 0.549589 | 3.1:-80276760 | MS.gene48459:intron |
| AGAGAGGCGAGGCCCGCCGA+AGG | 0.551697 | 3.1:+80276872 | None:intergenic |
| CTTTGAGGATGACGGAAACA+AGG | 0.557585 | 3.1:+80276904 | None:intergenic |
| CTTTGTCTTCCTTATAAACA+GGG | 0.557682 | 3.1:-80276123 | MS.gene48459:CDS |
| ACAAAGTCTAGTCGAGGACG+AGG | 0.560984 | 3.1:+80275724 | None:intergenic |
| ATTCTTCCAGATGCAAAACA+TGG | 0.562696 | 3.1:-80276069 | MS.gene48459:CDS |
| GACCAATGAAGTCAATTCAT+GGG | 0.562924 | 3.1:-80276382 | MS.gene48459:CDS |
| TATGCTATTGTGAGAGGCAA+GGG | 0.564855 | 3.1:-80275757 | MS.gene48459:CDS |
| TGAAACTTCTGATATGCTGA+AGG | 0.568879 | 3.1:-80275926 | MS.gene48459:CDS |
| GTTAAGTTGTTGACCAACCC+TGG | 0.572779 | 3.1:+80276318 | None:intergenic |
| ATTCTCAGTATCATCGCTGC+TGG | 0.573481 | 3.1:-80277008 | MS.gene48459:CDS |
| AGCAGAGTCTTTGAGGATGA+CGG | 0.575024 | 3.1:+80276896 | None:intergenic |
| CCAAATGAGACATTGAACCT+TGG | 0.576402 | 3.1:+80275952 | None:intergenic |
| CTATGCTATTGTGAGAGGCA+AGG | 0.580929 | 3.1:-80275758 | MS.gene48459:CDS |
| ATTAAAGGAGTTATTCAACC+AGG | 0.584761 | 3.1:-80276336 | MS.gene48459:CDS |
| AGCGATTGTGAGTGAAACGT+TGG | 0.585010 | 3.1:+80277094 | None:intergenic |
| TCTGATATGCTGAAGGACCT+AGG | 0.594866 | 3.1:-80275919 | MS.gene48459:CDS |
| GAGAGGCGAGGCCCGCCGAA+GGG | 0.597472 | 3.1:+80276873 | None:intergenic |
| TAGTATGATAACCCAGCAAG+AGG | 0.598459 | 3.1:+80275625 | None:intergenic |
| CGGAGGTTTGACAAATATGG+TGG | 0.603716 | 3.1:-80275875 | MS.gene48459:CDS |
| GGTTGATCATTGATTTACGA+CGG | 0.604361 | 3.1:+80277120 | None:intergenic |
| TCCAACAAAGAGGATTGTGC+CGG | 0.609638 | 3.1:+80275660 | None:intergenic |
| GACGGAGTTGAGTTGATCGA+TGG | 0.611829 | 3.1:+80276935 | None:intergenic |
| CTTGCCTCTCACAATAGCAT+AGG | 0.612056 | 3.1:+80275759 | None:intergenic |
| AATAGCATAGGTTGCAGCAG+CGG | 0.614189 | 3.1:+80275771 | None:intergenic |
| TTAAAGGAGTTATTCAACCA+GGG | 0.622511 | 3.1:-80276335 | MS.gene48459:CDS |
| TACCTTGTTCTGGAAATCAA+CGG | 0.624465 | 3.1:+80276758 | None:intergenic |
| TCTCAATGGTAGCTCACTCA+AGG | 0.625100 | 3.1:-80276205 | MS.gene48459:CDS |
| AGTCTAGTCGAGGACGAGGA+GGG | 0.627803 | 3.1:+80275728 | None:intergenic |
| TGAGTGAGCTACCATTGAGA+AGG | 0.638371 | 3.1:+80276208 | None:intergenic |
| AAGTCTAGTCGAGGACGAGG+AGG | 0.638727 | 3.1:+80275727 | None:intergenic |
| GCAACCTATGCTATTGTGAG+AGG | 0.638794 | 3.1:-80275763 | MS.gene48459:CDS |
| TGAGCACCTGTCCAACAAAG+AGG | 0.641694 | 3.1:+80275650 | None:intergenic |
| TCCGTTCATGAGCAGCAAAG+AGG | 0.648841 | 3.1:-80276181 | MS.gene48459:CDS |
| TAAAGTACATAGAGAATCGG+CGG | 0.649019 | 3.1:+80276091 | None:intergenic |
| AGAATAACTTGAATGGAGAG+AGG | 0.658718 | 3.1:+80277026 | None:intergenic |
| TAGGTGACTTCAGAATTCCA+AGG | 0.662092 | 3.1:-80275969 | MS.gene48459:CDS |
| TTATTAGAAATAAGAATGGG+TGG | 0.662615 | 3.1:+80275695 | None:intergenic |
| GATGACGGAAACAAGGCGAG+AGG | 0.683563 | 3.1:+80276911 | None:intergenic |
| ACACACCATTGGCGACAGAG+AGG | 0.687034 | 3.1:+80276856 | None:intergenic |
| TCAGCTACAAAGTCTAGTCG+AGG | 0.689387 | 3.1:+80275718 | None:intergenic |
| TATGCTGAAGGACCTAGGAG+TGG | 0.695951 | 3.1:-80275914 | MS.gene48459:CDS |
| GATCGATGGATTTGAAACGG+AGG | 0.718521 | 3.1:+80276949 | None:intergenic |
| CCATTGGCGACAGAGAGGCG+AGG | 0.720718 | 3.1:+80276861 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATAATTAGAAACAATT+CGG | + | chr3.1:80276178-80276197 | None:intergenic | 15.0% |
| !!! | GAAATTTGATATGAATTTTT+AGG | - | chr3.1:80276337-80276356 | MS.gene48459:CDS | 15.0% |
| !!! | TAATTTCATAAGTTTAGTTT+AGG | - | chr3.1:80276092-80276111 | MS.gene48459:CDS | 15.0% |
| !! | CTCTTATTAGAAATAAGAAT+GGG | + | chr3.1:80277063-80277082 | None:intergenic | 20.0% |
| !! | TCAATAATTAGAAACAATTC+GGG | + | chr3.1:80276177-80276196 | None:intergenic | 20.0% |
| !! | TCTCTTATTAGAAATAAGAA+TGG | + | chr3.1:80277064-80277083 | None:intergenic | 20.0% |
| !! | TTGGATGTTAAAAAACAATA+AGG | + | chr3.1:80276245-80276264 | None:intergenic | 20.0% |
| !!! | AAAATCATAGTCTTTAGTTT+TGG | + | chr3.1:80276522-80276541 | None:intergenic | 20.0% |
| ! | AATAAGAAAGTAAGCAATTG+AGG | + | chr3.1:80276040-80276059 | None:intergenic | 25.0% |
| ! | CATTATGTTGCTAAATTAGT+TGG | + | chr3.1:80276264-80276283 | None:intergenic | 25.0% |
| ! | CTTGAGAAAGATTAAACTTA+TGG | + | chr3.1:80276757-80276776 | None:intergenic | 25.0% |
| ! | GAATAAAGTACATAGAGAAT+CGG | + | chr3.1:80276667-80276686 | None:intergenic | 25.0% |
| ! | TTATTAGAAATAAGAATGGG+TGG | + | chr3.1:80277060-80277079 | None:intergenic | 25.0% |
| ! | TTTCGTTTCGTATCAAATTA+GGG | - | chr3.1:80276134-80276153 | MS.gene48459:CDS | 25.0% |
| !! | AAGGTATGTAATGTGTTTTA+GGG | - | chr3.1:80276011-80276030 | MS.gene48459:CDS | 25.0% |
| !! | CTAATAAGAGAAGATTTTAC+CGG | - | chr3.1:80277073-80277092 | MS.gene48459:CDS | 25.0% |
| AAACAATTCGGGAATGTTAT+TGG | + | chr3.1:80276166-80276185 | None:intergenic | 30.0% | |
| AACGAAACAATAAGCTAACA+TGG | + | chr3.1:80276121-80276140 | None:intergenic | 30.0% | |
| AATCTTTCTCAAGTCAAAGT+AGG | - | chr3.1:80276764-80276783 | MS.gene48459:CDS | 30.0% | |
| ACTTTGTCTTCCTTATAAAC+AGG | - | chr3.1:80276628-80276647 | MS.gene48459:intron | 30.0% | |
| AGCAAAGAGGATCAATATAT+TGG | - | chr3.1:80276584-80276603 | MS.gene48459:intron | 30.0% | |
| ATTAAAGGAGTTATTCAACC+AGG | - | chr3.1:80276416-80276435 | MS.gene48459:intron | 30.0% | |
| CTTTGTCTTCCTTATAAACA+GGG | - | chr3.1:80276629-80276648 | MS.gene48459:intron | 30.0% | |
| GATACTGAGAATAACTTGAA+TGG | + | chr3.1:80275736-80275755 | None:intergenic | 30.0% | |
| GTTTCGTTTCGTATCAAATT+AGG | - | chr3.1:80276133-80276152 | MS.gene48459:CDS | 30.0% | |
| TTAAAGGAGTTATTCAACCA+GGG | - | chr3.1:80276417-80276436 | MS.gene48459:intron | 30.0% | |
| ! | AGAAGAAAGAATCTTTTCGA+AGG | + | chr3.1:80275952-80275971 | None:intergenic | 30.0% |
| ! | CAAGGTATGTAATGTGTTTT+AGG | - | chr3.1:80276010-80276029 | MS.gene48459:CDS | 30.0% |
| ! | GAGACAAATGGACTTATTAA+AGG | - | chr3.1:80276401-80276420 | MS.gene48459:intron | 30.0% |
| ! | TATGATTTTCACCTTCTCAA+TGG | - | chr3.1:80276533-80276552 | MS.gene48459:intron | 30.0% |
| ! | TTACTTGGATGAAAGACTTT+TGG | + | chr3.1:80276943-80276962 | None:intergenic | 30.0% |
| !! | CAATATATTGGTGCTTTTGA+TGG | - | chr3.1:80276596-80276615 | MS.gene48459:intron | 30.0% |
| !!! | GAAAGACTTTTGGAATATCT+TGG | + | chr3.1:80276933-80276952 | None:intergenic | 30.0% |
| AAAAAGGTAAAACCACTCCT+AGG | + | chr3.1:80276853-80276872 | None:intergenic | 35.0% | |
| AATTCGGGAATGTTATTGGT+AGG | + | chr3.1:80276162-80276181 | None:intergenic | 35.0% | |
| ACACATTACATACCTTGTTC+TGG | + | chr3.1:80276007-80276026 | None:intergenic | 35.0% | |
| AGAATAACTTGAATGGAGAG+AGG | + | chr3.1:80275729-80275748 | None:intergenic | 35.0% | |
| ATTCTTCCAGATGCAAAACA+TGG | - | chr3.1:80276683-80276702 | MS.gene48459:intron | 35.0% | |
| CAAGGTTCAATGTCTCATTT+GGG | - | chr3.1:80276801-80276820 | MS.gene48459:CDS | 35.0% | |
| GACCAATGAAGTCAATTCAT+GGG | - | chr3.1:80276370-80276389 | MS.gene48459:CDS | 35.0% | |
| GCAAATGCACTGTACTTTAA+AGG | - | chr3.1:80276470-80276489 | MS.gene48459:intron | 35.0% | |
| TAAAGTACATAGAGAATCGG+CGG | + | chr3.1:80276664-80276683 | None:intergenic | 35.0% | |
| TGAAACTTCTGATATGCTGA+AGG | - | chr3.1:80276826-80276845 | MS.gene48459:CDS | 35.0% | |
| TGACCAATGAAGTCAATTCA+TGG | - | chr3.1:80276369-80276388 | MS.gene48459:CDS | 35.0% | |
| TTCATCCAAGTAAACGAAGA+AGG | - | chr3.1:80276950-80276969 | MS.gene48459:CDS | 35.0% | |
| ! | AACCCATGAATTGACTTCAT+TGG | + | chr3.1:80276375-80276394 | None:intergenic | 35.0% |
| ! | CACTGTACTTTAAAGGACTA+TGG | - | chr3.1:80276477-80276496 | MS.gene48459:intron | 35.0% |
| ! | GTTGATCGATGGATTTGAAA+CGG | + | chr3.1:80275809-80275828 | None:intergenic | 35.0% |
| ! | TGGGTTGAAAAAGAGACAAA+TGG | - | chr3.1:80276389-80276408 | MS.gene48459:CDS | 35.0% |
| !! | GGTTGATCATTGATTTACGA+CGG | + | chr3.1:80275635-80275654 | None:intergenic | 35.0% |
| !! | TACCTTGTTCTGGAAATCAA+CGG | + | chr3.1:80275997-80276016 | None:intergenic | 35.0% |
| AAAACATGGACTCTCAGCTT+TGG | - | chr3.1:80276697-80276716 | MS.gene48459:intron | 40.0% | |
| ACCATATTTGTCAAACCTCC+GGG | + | chr3.1:80276878-80276897 | None:intergenic | 40.0% | |
| CACCATATTTGTCAAACCTC+CGG | + | chr3.1:80276879-80276898 | None:intergenic | 40.0% | |
| CCAAATGAGACATTGAACCT+TGG | + | chr3.1:80276803-80276822 | None:intergenic | 40.0% | |
| CCAAGGTTCAATGTCTCATT+TGG | - | chr3.1:80276800-80276819 | MS.gene48459:CDS | 40.0% | |
| CGAAAACACGATGTTGTTGT+TGG | + | chr3.1:80275706-80275725 | None:intergenic | 40.0% | |
| TAGGTGACTTCAGAATTCCA+AGG | - | chr3.1:80276783-80276802 | MS.gene48459:CDS | 40.0% | |
| TAGTATGATAACCCAGCAAG+AGG | + | chr3.1:80277130-80277149 | None:intergenic | 40.0% | |
| TATGCTATTGTGAGAGGCAA+GGG | - | chr3.1:80276995-80277014 | MS.gene48459:CDS | 40.0% | |
| TCATCTTGGCCCTGTTTATA+AGG | + | chr3.1:80276641-80276660 | None:intergenic | 40.0% | |
| TGCATTTGCAAAGATGAGTC+TGG | + | chr3.1:80276459-80276478 | None:intergenic | 40.0% | |
| TTCTGATTTCCAAGCCAAGT+TGG | - | chr3.1:80275968-80275987 | MS.gene48459:CDS | 40.0% | |
| ! | ACTCTCAGCTTTGGTTGAAA+AGG | - | chr3.1:80276706-80276725 | MS.gene48459:intron | 40.0% |
| ! | TTGTGAGTGAAACGTTGGTT+TGG | + | chr3.1:80275656-80275675 | None:intergenic | 40.0% |
| !! | AATATGGTGGACTCTCTTGA+AGG | - | chr3.1:80276890-80276909 | MS.gene48459:CDS | 40.0% |
| !! | GTGGTTTTACCTTTTTCTCC+CGG | - | chr3.1:80276857-80276876 | MS.gene48459:CDS | 40.0% |
| !! | TTCCGTTGATTTCCAGAACA+AGG | - | chr3.1:80275992-80276011 | MS.gene48459:CDS | 40.0% |
| AATAGCATAGGTTGCAGCAG+CGG | + | chr3.1:80276984-80277003 | None:intergenic | 45.0% | |
| AGCGATTGTGAGTGAAACGT+TGG | + | chr3.1:80275661-80275680 | None:intergenic | 45.0% | |
| AGGAGTTATTCAACCAGGGT+TGG | - | chr3.1:80276421-80276440 | MS.gene48459:intron | 45.0% | |
| ATTCTCAGTATCATCGCTGC+TGG | - | chr3.1:80275744-80275763 | MS.gene48459:CDS | 45.0% | |
| CACAATCCTCTTTGTTGGAC+AGG | - | chr3.1:80277096-80277115 | MS.gene48459:CDS | 45.0% | |
| CAGTGCCTTCTTCGTTTACT+TGG | + | chr3.1:80276958-80276977 | None:intergenic | 45.0% | |
| CGGAGGTTTGACAAATATGG+TGG | - | chr3.1:80276877-80276896 | MS.gene48459:CDS | 45.0% | |
| CTATGCTATTGTGAGAGGCA+AGG | - | chr3.1:80276994-80277013 | MS.gene48459:CDS | 45.0% | |
| CTTGCCTCTCACAATAGCAT+AGG | + | chr3.1:80276996-80277015 | None:intergenic | 45.0% | |
| GAAATCAACGGAAGCCAACT+TGG | + | chr3.1:80275985-80276004 | None:intergenic | 45.0% | |
| GCAACCTATGCTATTGTGAG+AGG | - | chr3.1:80276989-80277008 | MS.gene48459:CDS | 45.0% | |
| TCAGCTACAAAGTCTAGTCG+AGG | + | chr3.1:80277037-80277056 | None:intergenic | 45.0% | |
| TCCAACAAAGAGGATTGTGC+CGG | + | chr3.1:80277095-80277114 | None:intergenic | 45.0% | |
| TCCCGGAGGTTTGACAAATA+TGG | - | chr3.1:80276874-80276893 | MS.gene48459:CDS | 45.0% | |
| TCCTCTTTGCTGCTCATGAA+CGG | + | chr3.1:80276575-80276594 | None:intergenic | 45.0% | |
| TCTCAATGGTAGCTCACTCA+AGG | - | chr3.1:80276547-80276566 | MS.gene48459:intron | 45.0% | |
| TCTGATATGCTGAAGGACCT+AGG | - | chr3.1:80276833-80276852 | MS.gene48459:CDS | 45.0% | |
| TGAGTGAGCTACCATTGAGA+AGG | + | chr3.1:80276547-80276566 | None:intergenic | 45.0% | |
| ! | AGCAGAGTCTTTGAGGATGA+CGG | + | chr3.1:80275859-80275878 | None:intergenic | 45.0% |
| ! | CTCAGCTTTGGTTGAAAAGG+TGG | - | chr3.1:80276709-80276728 | MS.gene48459:intron | 45.0% |
| ! | GAGAGTCCATGTTTTGCATC+TGG | + | chr3.1:80276692-80276711 | None:intergenic | 45.0% |
| ! | GATCGATGGATTTGAAACGG+AGG | + | chr3.1:80275806-80275825 | None:intergenic | 45.0% |
| ! | GTTAAGTTGTTGACCAACCC+TGG | + | chr3.1:80276437-80276456 | None:intergenic | 45.0% |
| ! | GTTTTACCTTTTTCTCCCGG+AGG | - | chr3.1:80276860-80276879 | MS.gene48459:CDS | 45.0% |
| !! | CTTTGAGGATGACGGAAACA+AGG | + | chr3.1:80275851-80275870 | None:intergenic | 45.0% |
| !! | GTCGAGAAGTTGTTGCTTTG+TGG | + | chr3.1:80275781-80275800 | None:intergenic | 45.0% |
| ACAAAGTCTAGTCGAGGACG+AGG | + | chr3.1:80277031-80277050 | None:intergenic | 50.0% | |
| ACCGGCACAATCCTCTTTGT+TGG | - | chr3.1:80277091-80277110 | MS.gene48459:CDS | 50.0% | |
| AGGTGCTCAATCCTCTTGCT+GGG | - | chr3.1:80277116-80277135 | MS.gene48459:CDS | 50.0% | |
| CTCAAAGACTCTGCTCCCTT+CGG | - | chr3.1:80275864-80275883 | MS.gene48459:CDS | 50.0% | |
| GTCAAACCTCCGGGAGAAAA+AGG | + | chr3.1:80276869-80276888 | None:intergenic | 50.0% | |
| TAGAGAATCGGCGGTCATCT+TGG | + | chr3.1:80276655-80276674 | None:intergenic | 50.0% | |
| TATGCTGAAGGACCTAGGAG+TGG | - | chr3.1:80276838-80276857 | MS.gene48459:CDS | 50.0% | |
| TCCGTTCATGAGCAGCAAAG+AGG | - | chr3.1:80276571-80276590 | MS.gene48459:intron | 50.0% | |
| TGAGCACCTGTCCAACAAAG+AGG | + | chr3.1:80277105-80277124 | None:intergenic | 50.0% | |
| TTGCTCAACCCACACACCAT+TGG | + | chr3.1:80275910-80275929 | None:intergenic | 50.0% | |
| !! | GACGGAGTTGAGTTGATCGA+TGG | + | chr3.1:80275820-80275839 | None:intergenic | 50.0% |
| AAAGACTCTGCTCCCTTCGG+CGG | - | chr3.1:80275867-80275886 | MS.gene48459:CDS | 55.0% | |
| AAGTCTAGTCGAGGACGAGG+AGG | + | chr3.1:80277028-80277047 | None:intergenic | 55.0% | |
| ACACACCATTGGCGACAGAG+AGG | + | chr3.1:80275899-80275918 | None:intergenic | 55.0% | |
| AGTCTAGTCGAGGACGAGGA+GGG | + | chr3.1:80277027-80277046 | None:intergenic | 55.0% | |
| CAACGGAAGCCAACTTGGCT+TGG | + | chr3.1:80275980-80275999 | None:intergenic | 55.0% | |
| CAGGTGCTCAATCCTCTTGC+TGG | - | chr3.1:80277115-80277134 | MS.gene48459:CDS | 55.0% | |
| CGAAGGGAGCAGAGTCTTTG+AGG | + | chr3.1:80275866-80275885 | None:intergenic | 55.0% | |
| CTCTGTCGCCAATGGTGTGT+GGG | - | chr3.1:80275899-80275918 | MS.gene48459:CDS | 55.0% | |
| TCTCTGTCGCCAATGGTGTG+TGG | - | chr3.1:80275898-80275917 | MS.gene48459:CDS | 55.0% | |
| !! | ATCATCGCTGCTGGCTCTGA+GGG | - | chr3.1:80275753-80275772 | MS.gene48459:CDS | 55.0% |
| !! | GATGACGGAAACAAGGCGAG+AGG | + | chr3.1:80275844-80275863 | None:intergenic | 55.0% |
| !! | TATCATCGCTGCTGGCTCTG+AGG | - | chr3.1:80275752-80275771 | MS.gene48459:CDS | 55.0% |
| AAGACTCTGCTCCCTTCGGC+GGG | - | chr3.1:80275868-80275887 | MS.gene48459:CDS | 60.0% | |
| GGAAACAAGGCGAGAGGCGA+CGG | + | chr3.1:80275838-80275857 | None:intergenic | 60.0% | |
| CCATTGGCGACAGAGAGGCG+AGG | + | chr3.1:80275894-80275913 | None:intergenic | 65.0% | |
| CCTCGCCTCTCTGTCGCCAA+TGG | - | chr3.1:80275891-80275910 | MS.gene48459:CDS | 65.0% | |
| AGAGAGGCGAGGCCCGCCGA+AGG | + | chr3.1:80275883-80275902 | None:intergenic | 75.0% | |
| GAGAGGCGAGGCCCGCCGAA+GGG | + | chr3.1:80275882-80275901 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 80275624 | 80277150 | 80275624 | ID=MS.gene48459 |
| chr3.1 | mRNA | 80275624 | 80277150 | 80275624 | ID=MS.gene48459.t1;Parent=MS.gene48459 |
| chr3.1 | exon | 80276761 | 80277150 | 80276761 | ID=MS.gene48459.t1.exon1;Parent=MS.gene48459.t1 |
| chr3.1 | CDS | 80276761 | 80277150 | 80276761 | ID=cds.MS.gene48459.t1;Parent=MS.gene48459.t1 |
| chr3.1 | exon | 80275624 | 80276415 | 80275624 | ID=MS.gene48459.t1.exon2;Parent=MS.gene48459.t1 |
| chr3.1 | CDS | 80275624 | 80276415 | 80275624 | ID=cds.MS.gene48459.t1;Parent=MS.gene48459.t1 |
| Gene Sequence |
| Protein sequence |
