AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49005


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49005.t1 MTR_7g056393 83.333 180 30 0 1 180 1 180 1.39e-105 311
MS.gene49005.t1 MTR_7g056393 91.954 87 7 0 174 260 287 373 2.98e-47 161
MS.gene49005.t1 MTR_4g119410 81.111 180 34 0 1 180 1 180 1.58e-104 308
MS.gene49005.t1 MTR_4g119410 90.000 80 8 0 181 260 294 373 8.85e-43 149
MS.gene49005.t1 MTR_8g006930 73.096 197 53 0 1 197 1 197 1.49e-103 305
MS.gene49005.t1 MTR_8g006930 85.185 81 12 0 180 260 293 373 1.36e-41 146
MS.gene49005.t1 MTR_7g078010 72.680 194 53 0 1 194 1 194 4.72e-103 301
MS.gene49005.t1 MTR_7g078010 72.680 194 53 0 1 194 1 194 5.54e-102 301
MS.gene49005.t1 MTR_7g078010 73.563 87 23 0 174 260 287 373 6.54e-36 131
MS.gene49005.t1 MTR_7g056347 67.513 197 64 0 1 197 1 197 2.39e-91 275
MS.gene49005.t1 MTR_7g056347 75.309 81 20 0 180 260 293 373 2.32e-36 132
MS.gene49005.t1 MTR_8g063070 60.000 180 72 0 1 180 1 180 2.17e-75 234
MS.gene49005.t1 MTR_8g063070 58.750 80 33 0 181 260 294 373 3.15e-24 100
MS.gene49005.t1 MTR_8g062600 55.556 198 88 0 1 198 1 198 1.23e-73 229
MS.gene49005.t1 MTR_8g062600 59.770 87 35 0 174 260 287 373 1.52e-27 109
MS.gene49005.t1 MTR_4g024660 60.556 180 71 0 1 180 1 180 8.15e-73 227
MS.gene49005.t1 MTR_4g024660 59.770 87 35 0 174 260 287 373 1.29e-28 112
MS.gene49005.t1 MTR_1g093120 60.335 179 65 3 8 180 2 180 1.58e-71 224
MS.gene49005.t1 MTR_1g093120 68.750 80 22 1 181 260 294 370 3.95e-30 116
MS.gene49005.t1 MTR_1g093140 58.201 189 67 3 1 180 1 186 4.29e-71 223
MS.gene49005.t1 MTR_1g093140 72.500 80 21 1 181 260 300 378 2.57e-31 119
MS.gene49005.t1 MTR_1g093110 56.842 190 76 2 11 194 8 197 1.84e-70 221
MS.gene49005.t1 MTR_1g093110 72.500 80 20 1 181 260 297 374 5.24e-32 121
MS.gene49005.t1 MTR_7g056370 52.941 187 87 1 27 212 6 192 1.76e-67 208
MS.gene49005.t1 MTR_2g069010 58.788 165 66 1 17 179 17 181 1.27e-62 201
MS.gene49005.t1 MTR_2g069010 56.818 88 36 2 174 260 289 375 8.42e-23 96.3
MS.gene49005.t1 MTR_2g068960 57.576 165 68 1 17 179 11 175 6.14e-62 199
MS.gene49005.t1 MTR_2g068960 56.098 82 34 2 180 260 289 369 4.22e-20 88.6
MS.gene49005.t1 MTR_2g068950 58.182 165 67 1 17 179 11 175 7.42e-62 199
MS.gene49005.t1 MTR_2g068950 56.098 82 34 2 180 260 289 369 3.21e-20 89.0
MS.gene49005.t1 MTR_2g069020 56.897 174 72 2 17 187 15 188 1.33e-61 198
MS.gene49005.t1 MTR_2g069020 57.143 70 28 2 180 249 293 360 7.60e-18 82.4
MS.gene49005.t1 MTR_8g469400 54.011 187 74 1 1 187 1 175 2.94e-61 197
MS.gene49005.t1 MTR_8g469400 58.621 87 36 0 174 260 274 360 3.62e-28 110
MS.gene49005.t1 MTR_2g069020 56.897 174 72 2 17 187 15 188 4.29e-61 197
MS.gene49005.t1 MTR_2g069020 57.143 70 28 2 180 249 293 360 1.07e-17 82.0
MS.gene49005.t1 MTR_4g099380 54.268 164 68 2 19 180 124 282 1.34e-57 185
MS.gene49005.t1 MTR_2g069060 47.087 206 79 2 1 194 1 188 9.53e-57 185
MS.gene49005.t1 MTR_2g069060 60.494 81 26 2 180 260 275 349 1.66e-23 97.8
MS.gene49005.t1 MTR_2g069070 47.449 196 85 1 1 196 1 178 5.32e-54 173
MS.gene49005.t1 MTR_8g041600 40.102 197 115 2 1 194 1 197 6.15e-52 171
MS.gene49005.t1 MTR_8g041620 41.117 197 113 2 1 194 1 197 6.66e-52 173
MS.gene49005.t1 MTR_8g041620 56.790 81 33 2 180 260 296 374 1.53e-22 95.5
MS.gene49005.t1 MTR_8g041600 40.102 197 115 2 1 194 1 197 2.72e-51 172
MS.gene49005.t1 MTR_8g041600 59.259 81 31 2 180 260 296 374 8.68e-24 99.0
MS.gene49005.t1 MTR_8g009080 45.856 181 97 1 1 180 27 207 1.86e-48 163
MS.gene49005.t1 MTR_8g009080 45.856 181 97 1 1 180 27 207 2.64e-48 163
MS.gene49005.t1 MTR_8g009080 45.856 181 97 1 1 180 27 207 6.72e-48 163
MS.gene49005.t1 MTR_8g009080 50.617 81 37 1 180 260 320 397 9.93e-20 87.8
MS.gene49005.t1 MTR_8g009090 43.750 192 98 4 6 194 4 188 1.18e-47 162
MS.gene49005.t1 MTR_8g009090 55.556 81 33 1 180 260 287 364 1.08e-21 93.2
MS.gene49005.t1 MTR_8g008930 43.299 194 99 5 5 194 3 189 1.81e-46 159
MS.gene49005.t1 MTR_8g008930 51.852 81 36 1 180 260 288 365 7.76e-20 87.8
MS.gene49005.t1 MTR_2g069210 44.000 175 89 2 11 180 7 177 3.20e-45 155
MS.gene49005.t1 MTR_2g069210 61.728 81 28 1 180 260 290 367 2.60e-22 94.7
MS.gene49005.t1 MTR_8g028435 42.614 176 94 3 22 194 17 188 1.52e-44 154
MS.gene49005.t1 MTR_8g028435 56.790 81 32 1 180 260 287 364 6.17e-24 99.4
MS.gene49005.t1 MTR_1g043760 44.785 163 89 1 18 180 5 166 2.05e-44 153
MS.gene49005.t1 MTR_1g043760 45.946 74 37 1 180 253 279 349 1.28e-12 67.0
MS.gene49005.t1 MTR_2g069300 45.455 165 87 2 18 180 4 167 3.87e-44 152
MS.gene49005.t1 MTR_2g069300 51.852 81 36 1 180 260 281 358 3.79e-18 83.2
MS.gene49005.t1 MTR_1g070185 44.242 165 89 2 17 180 18 180 8.91e-44 152
MS.gene49005.t1 MTR_1g070185 41.975 81 41 1 180 260 293 367 1.46e-11 63.9
MS.gene49005.t1 MTR_2g069060 48.649 148 64 1 59 194 19 166 2.17e-43 148
MS.gene49005.t1 MTR_1g044105 42.857 168 89 3 18 179 5 171 2.20e-43 145
MS.gene49005.t1 MTR_8g009170 41.243 177 96 4 22 194 17 189 5.71e-43 148
MS.gene49005.t1 MTR_8g009160 41.243 177 97 3 21 194 16 188 1.57e-42 149
MS.gene49005.t1 MTR_8g009160 51.852 81 36 1 180 260 287 364 1.63e-21 92.4
MS.gene49005.t1 MTR_8g009170 41.243 177 96 4 22 194 17 189 1.72e-42 149
MS.gene49005.t1 MTR_8g009170 54.878 82 33 2 180 260 288 366 6.72e-22 93.6
MS.gene49005.t1 MTR_8g009110 40.341 176 101 4 21 194 16 189 4.32e-42 147
MS.gene49005.t1 MTR_8g009110 54.321 81 34 1 180 260 288 365 1.84e-21 92.4
MS.gene49005.t1 MTR_1g043770 41.860 172 96 3 13 184 2 169 1.80e-41 144
MS.gene49005.t1 MTR_1g043770 41.860 172 96 3 13 184 2 169 3.25e-41 145
MS.gene49005.t1 MTR_1g043770 44.444 81 42 1 180 260 278 355 5.06e-15 73.9
MS.gene49005.t1 MTR_2g069190 43.182 176 97 2 7 180 7 181 1.19e-40 144
MS.gene49005.t1 MTR_2g069190 55.952 84 28 2 180 260 294 371 8.74e-21 90.5
MS.gene49005.t1 MTR_8g009150 41.243 177 96 4 22 194 17 189 1.28e-40 143
MS.gene49005.t1 MTR_8g009150 50.617 81 37 1 180 260 268 345 1.94e-19 86.7
MS.gene49005.t1 MTR_2g069090 39.175 194 109 2 6 194 6 195 1.52e-40 144
MS.gene49005.t1 MTR_2g069090 59.259 81 30 1 180 260 295 372 1.29e-22 95.9
MS.gene49005.t1 MTR_1g043810 41.463 164 94 2 18 180 5 167 6.41e-40 141
MS.gene49005.t1 MTR_1g043810 41.463 164 94 2 18 180 5 167 1.24e-39 140
MS.gene49005.t1 MTR_1g043810 50.667 75 34 1 181 255 281 352 9.18e-17 79.0
MS.gene49005.t1 MTR_4g117880 42.328 189 100 5 11 194 5 189 1.90e-39 140
MS.gene49005.t1 MTR_4g117880 48.780 82 38 2 180 260 288 366 1.30e-15 75.9
MS.gene49005.t1 MTR_8g009120 43.827 162 83 4 22 179 17 174 2.22e-39 140
MS.gene49005.t1 MTR_8g009120 54.217 83 35 1 178 260 274 353 2.62e-22 94.7
MS.gene49005.t1 MTR_0032s0040 39.691 194 102 5 12 194 7 196 5.31e-39 139
MS.gene49005.t1 MTR_0032s0040 58.025 81 30 2 181 260 296 373 5.91e-19 85.5
MS.gene49005.t1 MTR_7g029655 37.640 178 103 2 12 184 7 181 6.98e-38 136
MS.gene49005.t1 MTR_7g029655 53.933 89 37 3 174 260 284 370 1.80e-18 84.0
MS.gene49005.t1 MTR_7g105070 41.916 167 95 2 15 180 6 171 8.37e-38 136
MS.gene49005.t1 MTR_7g105070 41.975 81 43 2 181 260 285 362 2.04e-11 63.5
MS.gene49005.t1 MTR_1g043775 40.854 164 95 2 18 180 5 167 1.35e-37 134
MS.gene49005.t1 MTR_1g043775 40.854 164 95 2 18 180 5 167 2.71e-37 134
MS.gene49005.t1 MTR_1g043775 49.333 75 35 1 181 255 281 352 1.60e-15 75.5
MS.gene49005.t1 MTR_1g032220 41.317 167 91 4 17 180 21 183 2.33e-36 132
MS.gene49005.t1 MTR_1g032250 38.889 180 101 5 6 180 8 183 1.98e-34 127
MS.gene49005.t1 MTR_1g032140 40.120 167 93 4 17 180 14 176 2.83e-34 127
MS.gene49005.t1 MTR_4g099390 70.000 80 23 1 181 260 6 84 2.21e-33 117
MS.gene49005.t1 MTR_7g056353 38.172 186 86 4 6 190 8 165 1.80e-31 121
MS.gene49005.t1 MTR_7g056353 63.291 79 29 0 181 259 268 346 3.72e-25 103
MS.gene49005.t1 MTR_8g008960 50.667 75 34 1 186 260 31 102 4.56e-21 85.5
MS.gene49005.t1 MTR_2g069130 51.579 95 40 2 71 163 12 102 1.07e-20 85.5
MS.gene49005.t1 MTR_8g009180 51.316 76 34 1 180 255 217 289 7.02e-19 84.3
MS.gene49005.t1 MTR_1g062110 32.168 143 90 4 32 169 26 166 5.01e-16 77.0
MS.gene49005.t1 MTR_1g062110 32.168 143 90 4 32 169 26 166 5.13e-16 76.6
MS.gene49005.t1 MTR_1g032230 32.877 146 72 4 12 154 10 132 4.73e-15 73.2
MS.gene49005.t1 MTR_7g102820 32.432 148 85 7 32 169 25 167 1.03e-14 73.6
MS.gene49005.t1 MTR_3g091350 28.261 184 105 7 1 172 1 169 3.96e-12 65.9
MS.gene49005.t1 MTR_7g068650 29.310 116 76 2 23 132 5 120 5.60e-12 65.5
MS.gene49005.t1 MTR_5g055820 28.105 153 95 5 32 178 12 155 7.68e-12 64.7
MS.gene49005.t1 MTR_1g043840 39.726 73 43 1 78 150 257 328 4.46e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49005.t1 AT1G06650 44.809 183 96 2 17 194 10 192 2.63e-50 167
MS.gene49005.t1 AT1G06650 44.809 183 96 2 17 194 10 192 1.30e-49 167
MS.gene49005.t1 AT1G06650 48.780 82 38 2 180 260 291 369 1.46e-16 78.6
MS.gene49005.t1 AT1G06640 45.856 181 91 3 11 184 2 182 6.48e-49 163
MS.gene49005.t1 AT1G06640 46.471 170 86 2 16 180 9 178 1.42e-48 163
MS.gene49005.t1 AT2G30840 50.296 169 80 2 15 179 3 171 3.60e-48 162
MS.gene49005.t1 AT1G06640 45.977 174 89 2 16 184 9 182 4.07e-48 163
MS.gene49005.t1 AT1G06640 47.561 82 39 2 180 260 291 369 2.01e-15 75.5
MS.gene49005.t1 AT2G30840 50.296 169 80 2 15 179 3 171 7.36e-48 162
MS.gene49005.t1 AT2G30840 55.682 88 32 4 175 260 274 356 2.19e-18 84.0
MS.gene49005.t1 AT2G30840 50.296 169 80 2 15 179 3 171 7.86e-48 162
MS.gene49005.t1 AT2G30840 57.831 83 28 4 180 260 285 362 5.44e-18 82.8
MS.gene49005.t1 AT1G06620 46.386 166 88 1 15 179 9 174 7.91e-48 160
MS.gene49005.t1 AT2G30830 49.091 165 82 2 17 180 5 168 2.39e-47 161
MS.gene49005.t1 AT2G30830 50.602 83 34 3 180 260 281 358 2.29e-15 75.1
MS.gene49005.t1 AT1G06620 46.386 166 88 1 15 179 9 174 5.65e-47 160
MS.gene49005.t1 AT1G06620 53.947 76 32 1 180 255 288 360 7.32e-18 82.4
MS.gene49005.t1 AT2G25450 43.558 163 92 0 17 179 5 167 9.78e-46 157
MS.gene49005.t1 AT2G25450 51.807 83 34 4 180 260 281 359 1.17e-13 70.1
MS.gene49005.t1 AT1G03410 43.976 166 89 2 18 179 5 170 5.42e-44 152
MS.gene49005.t1 AT1G03410 42.529 174 96 2 10 179 34 207 1.31e-43 152
MS.gene49005.t1 AT1G06645 45.912 159 81 2 16 169 22 180 4.56e-43 150
MS.gene49005.t1 AT1G06645 48.780 82 38 2 180 260 301 379 1.20e-16 79.0
MS.gene49005.t1 AT5G59540 42.857 168 92 3 17 180 9 176 3.48e-42 145
MS.gene49005.t1 AT5G59540 42.857 168 92 3 17 180 9 176 1.48e-41 146
MS.gene49005.t1 AT5G59540 58.025 81 31 1 180 260 289 366 5.43e-22 94.0
MS.gene49005.t1 AT1G03400 37.363 182 104 2 18 197 5 178 1.42e-39 140
MS.gene49005.t1 AT1G03400 37.363 182 104 2 18 197 5 178 4.77e-39 140
MS.gene49005.t1 AT5G59530 39.521 167 98 3 17 180 8 174 1.55e-38 138
MS.gene49005.t1 AT5G59530 55.556 81 33 1 180 260 287 364 2.31e-21 92.0
MS.gene49005.t1 AT1G04350 38.922 167 96 3 17 180 6 169 1.73e-38 138
MS.gene49005.t1 AT1G04350 53.659 82 34 2 180 260 282 360 1.21e-17 81.6
MS.gene49005.t1 AT3G61400 31.282 195 128 2 9 197 1 195 3.98e-35 127
MS.gene49005.t1 AT3G61400 31.282 195 128 2 9 197 1 195 1.99e-34 127
MS.gene49005.t1 AT3G61400 44.578 83 42 2 174 255 287 366 2.47e-13 69.3
MS.gene49005.t1 AT5G43440 38.012 171 100 4 19 184 10 179 1.10e-33 124
MS.gene49005.t1 AT5G43440 38.012 171 100 4 19 184 10 179 3.84e-33 124
MS.gene49005.t1 AT5G43440 49.383 81 38 1 180 260 288 365 3.43e-15 74.7
MS.gene49005.t1 AT5G43450 37.952 166 98 3 18 180 9 172 5.19e-33 122
MS.gene49005.t1 AT5G43450 37.952 166 98 3 18 180 9 172 1.47e-32 122
MS.gene49005.t1 AT5G43450 52.632 76 33 1 180 255 285 357 1.82e-14 72.8
MS.gene49005.t1 AT1G04380 36.691 139 84 2 42 180 21 155 1.01e-26 105
MS.gene49005.t1 AT1G04380 36.691 139 84 2 42 180 21 155 2.28e-26 105
MS.gene49005.t1 AT1G04380 55.556 81 33 1 180 260 268 345 2.55e-21 91.7
MS.gene49005.t1 AT2G44800 27.273 143 101 2 19 159 2 143 7.58e-13 67.8
MS.gene49005.t1 AT2G36690 30.556 144 92 4 32 169 20 161 1.67e-12 67.0
MS.gene49005.t1 AT2G36690 30.556 144 92 4 32 169 20 161 1.67e-12 67.0
MS.gene49005.t1 AT3G60290 30.952 126 84 2 36 159 19 143 1.73e-11 63.9
MS.gene49005.t1 AT4G10490 33.036 112 73 2 66 177 41 150 1.93e-11 63.5
MS.gene49005.t1 AT5G24530 29.801 151 86 4 41 187 23 157 4.43e-11 62.8

Find 58 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTGATCTCCAACTTTC+CGG 0.217968 7.3:+48557026 None:intergenic
AAAGGTTTATGGTCCTATTA+AGG 0.275771 7.3:-48555176 MS.gene49005:CDS
CGAAATTTCCACTATTATCT+TGG 0.281145 7.3:-48556863 MS.gene49005:CDS
GTCAGAGGATTGGTAGAGTC+TGG 0.290115 7.3:-48557078 MS.gene49005:CDS
GAAGGTACATCAAAGGTTTA+TGG 0.290841 7.3:-48555187 MS.gene49005:CDS
TTAGCGGCCAAGCTGCTAAT+TGG 0.291740 7.3:-48556726 MS.gene49005:CDS
CATATGGATGGTAGTTCATT+TGG 0.297189 7.3:+48556661 None:intergenic
TCTGAAAGGAGCTCCTTAAT+AGG 0.307082 7.3:+48555163 None:intergenic
CATGGCATGCGGTTCGAATT+TGG 0.319624 7.3:+48556914 None:intergenic
AAGCTTGCTACCGAAATTCT+TGG 0.320171 7.3:+48555235 None:intergenic
CCTAGATGGAAACTCTTTCT+TGG 0.320238 7.3:-48555074 MS.gene49005:CDS
GGTAGTTCATTTGGTTTGAA+TGG 0.352643 7.3:+48556670 None:intergenic
GAACATTGGCCCAAGAATTT+CGG 0.356167 7.3:-48555245 MS.gene49005:CDS
GATTGATGTGTATGTCTTTG+AGG 0.364574 7.3:+48556962 None:intergenic
CTTAGTTAATTACCTGCATA+TGG 0.366858 7.3:+48556645 None:intergenic
TATTATGCAAAAGGCCTAGA+TGG 0.373788 7.3:-48555088 MS.gene49005:CDS
ATGGTAATGTCTTTGTAGAT+TGG 0.378270 7.3:+48555130 None:intergenic
TCTATCATAAGTGGAATTAG+TGG 0.395303 7.3:+48557140 None:intergenic
ACCTTTGATGTACCTTCAAT+TGG 0.399600 7.3:+48555193 None:intergenic
ATCTTGGATGAAATGATTGA+TGG 0.408517 7.3:-48556847 MS.gene49005:CDS
GGTAGAAAACACAAATCAAT+TGG 0.412811 7.3:-48557169 MS.gene49005:CDS
TATGTCTTTGAGGTCTATAA+TGG 0.413542 7.3:+48556972 None:intergenic
TTTGCACATTATTATGCAAA+AGG 0.427901 7.3:-48555097 MS.gene49005:CDS
AGCTTGCTACCGAAATTCTT+GGG 0.447441 7.3:+48555236 None:intergenic
TCAGAGGATTGGTAGAGTCT+GGG 0.462084 7.3:-48557077 MS.gene49005:CDS
ATGTTGAATTCACAACTTGA+AGG 0.473909 7.3:+48555048 None:intergenic
TCCAATGGAACCTTGTTTAG+CGG 0.475131 7.3:-48556742 MS.gene49005:CDS
TCATTTGGTTTGAATGGATG+AGG 0.483777 7.3:+48556676 None:intergenic
GCCGCTAAACAAGGTTCCAT+TGG 0.487811 7.3:+48556741 None:intergenic
CCAAGAAAGAGTTTCCATCT+AGG 0.487963 7.3:+48555074 None:intergenic
GTAATGTCTTTGTAGATTGG+AGG 0.488313 7.3:+48555133 None:intergenic
AAAGTTATGTATTTCTCCAA+TGG 0.490517 7.3:-48556757 MS.gene49005:CDS
CAATCCAGCTCTACGTATAG+AGG 0.493480 7.3:-48556944 MS.gene49005:CDS
AAAAGTTGGTGTCAGAGGAT+TGG 0.504230 7.3:-48557088 MS.gene49005:CDS
CATTTCATCCAAGATAATAG+TGG 0.505113 7.3:+48556855 None:intergenic
ATGTCTTTGAGGTCTATAAT+GGG 0.505465 7.3:+48556973 None:intergenic
AATTCTCATGAACCAATTGA+AGG 0.506027 7.3:-48555205 MS.gene49005:CDS
TATAATGGGAACACTCAACT+TGG 0.531180 7.3:+48556987 None:intergenic
GTTAATTACCTGCATATGGA+TGG 0.532420 7.3:+48556649 None:intergenic
ATCACCTCTATACGTAGAGC+TGG 0.533602 7.3:+48556940 None:intergenic
TAATGTCTTTGTAGATTGGA+GGG 0.537461 7.3:+48555134 None:intergenic
ATGAACTACCATCCATATGC+AGG 0.538470 7.3:-48556657 MS.gene49005:intron
TTCGAACCGCATGCCATGAG+TGG 0.545312 7.3:-48556909 MS.gene49005:CDS
AGCAGCTTGGCCGCTAAACA+AGG 0.553269 7.3:+48556732 None:intergenic
GATGAAATGATTGATGGAAT+CGG 0.555039 7.3:-48556841 MS.gene49005:CDS
TATCTCTCCAATTAGCAGCT+TGG 0.556726 7.3:+48556719 None:intergenic
CAATGTTCTGTGATAAGACT+CGG 0.558348 7.3:+48555260 None:intergenic
TGTTGAATTCACAACTTGAA+GGG 0.564135 7.3:+48555049 None:intergenic
TCGAACCGCATGCCATGAGT+GGG 0.565520 7.3:-48556908 MS.gene49005:CDS
GATTCAAAAGTTGGTGTCAG+AGG 0.566152 7.3:-48557093 MS.gene49005:CDS
ACCAATTGAAGGTACATCAA+AGG 0.570655 7.3:-48555194 MS.gene49005:CDS
AGAAAACACAAATCAATTGG+AGG 0.577651 7.3:-48557166 MS.gene49005:CDS
CAGAGGATTGGTAGAGTCTG+GGG 0.587912 7.3:-48557076 MS.gene49005:CDS
GCTAATTGGAGAGATACACT+TGG 0.617281 7.3:-48556712 MS.gene49005:CDS
TTTGAATGGATGAGGAGCAA+CGG 0.642538 7.3:+48556684 None:intergenic
CGAGTCTTATCACAGAACAT+TGG 0.652569 7.3:-48555259 MS.gene49005:CDS
AAATCCCCACTCATGGCATG+CGG 0.677597 7.3:+48556903 None:intergenic
CGAACCGCATGCCATGAGTG+GGG 0.694194 7.3:-48556907 MS.gene49005:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTCATTATAAATTGAT+GGG + chr7.3:48555629-48555648 None:intergenic 15.0%
!! AAAATATTCCCAAAAAATAA+GGG - chr7.3:48556262-48556281 MS.gene49005:intron 15.0%
!! AAATCAAACATTAATAGATA+TGG - chr7.3:48556008-48556027 MS.gene49005:intron 15.0%
!! ATAAAATATTCCAAAAGATA+AGG - chr7.3:48556201-48556220 MS.gene49005:intron 15.0%
!! TAAAATATTCCAAAAGATAA+GGG - chr7.3:48556202-48556221 MS.gene49005:intron 15.0%
!! TAAAATATTCCCAAAAAATA+AGG - chr7.3:48556261-48556280 MS.gene49005:intron 15.0%
!!! ATCAGTTATATTTTTTTGTA+AGG + chr7.3:48556734-48556753 None:intergenic 15.0%
!!! TCAGTTATATTTTTTTGTAA+GGG + chr7.3:48556733-48556752 None:intergenic 15.0%
!! AGTAATATACTAATATGCAA+TGG + chr7.3:48556788-48556807 None:intergenic 20.0%
!! CATAATCTTTGAATTATTGT+CGG - chr7.3:48556295-48556314 MS.gene49005:intron 20.0%
!! GAAAACTCATTATAAATTGA+TGG + chr7.3:48555630-48555649 None:intergenic 20.0%
!! TAATTTGAAGGAAAAAAACT+CGG - chr7.3:48555945-48555964 MS.gene49005:intron 20.0%
! AAAGTTATGTATTTCTCCAA+TGG - chr7.3:48555474-48555493 MS.gene49005:intron 25.0%
! AATTATAATTGTCCTCAAGA+CGG - chr7.3:48556678-48556697 MS.gene49005:CDS 25.0%
! ATTATAGTGAACTGCAATTA+TGG - chr7.3:48556819-48556838 MS.gene49005:CDS 25.0%
! TAACTACGAGTATAATTTGA+AGG - chr7.3:48555933-48555952 MS.gene49005:intron 25.0%
! TTATAGTGAACTGCAATTAT+GGG - chr7.3:48556820-48556839 MS.gene49005:CDS 25.0%
! TTCAAATTATACTCGTAGTT+AGG + chr7.3:48555934-48555953 None:intergenic 25.0%
! TTGACTTAAACAATGACAAA+GGG + chr7.3:48556587-48556606 None:intergenic 25.0%
! TTTCAAATGGACATGATATA+TGG + chr7.3:48556159-48556178 None:intergenic 25.0%
! TTTGCACATTATTATGCAAA+AGG - chr7.3:48557134-48557153 MS.gene49005:CDS 25.0%
!! ATGTGCAAAGAAATCTTTTA+TGG + chr7.3:48557123-48557142 None:intergenic 25.0%
!! ATTGACTTAAACAATGACAA+AGG + chr7.3:48556588-48556607 None:intergenic 25.0%
!! CATTTGAAATATTCCCAAAA+GGG - chr7.3:48556170-48556189 MS.gene49005:intron 25.0%
!! TGAAGTTATGCATAATCTTT+TGG - chr7.3:48556612-48556631 MS.gene49005:intron 25.0%
!!! TTAGGAAAATGCTATTTCAA+GGG - chr7.3:48555659-48555678 MS.gene49005:intron 25.0%
!!! TTTAGGAAAATGCTATTTCA+AGG - chr7.3:48555658-48555677 MS.gene49005:intron 25.0%
AAAGAGTTGAATTGTTGTGA+AGG - chr7.3:48556347-48556366 MS.gene49005:intron 30.0%
AAAGGTTTATGGTCCTATTA+AGG - chr7.3:48557055-48557074 MS.gene49005:CDS 30.0%
AAGTTCTTCATGAGGATAAA+TGG - chr7.3:48556492-48556511 MS.gene49005:intron 30.0%
AATCTTTGAATTATTGTCGG+AGG - chr7.3:48556298-48556317 MS.gene49005:intron 30.0%
AATTCTCATGAACCAATTGA+AGG - chr7.3:48557026-48557045 MS.gene49005:CDS 30.0%
AGAAAACACAAATCAATTGG+AGG - chr7.3:48555065-48555084 MS.gene49005:CDS 30.0%
ATCTTGGATGAAATGATTGA+TGG - chr7.3:48555384-48555403 MS.gene49005:intron 30.0%
ATTCCAAAAGATAAGGGATT+TGG - chr7.3:48556208-48556227 MS.gene49005:intron 30.0%
CATTTCATCCAAGATAATAG+TGG + chr7.3:48555379-48555398 None:intergenic 30.0%
CGAAATTTCCACTATTATCT+TGG - chr7.3:48555368-48555387 MS.gene49005:intron 30.0%
CTTAGTTAATTACCTGCATA+TGG + chr7.3:48555589-48555608 None:intergenic 30.0%
GAAGACTCATGAATGTATAA+TGG + chr7.3:48555908-48555927 None:intergenic 30.0%
GATGAAATGATTGATGGAAT+CGG - chr7.3:48555390-48555409 MS.gene49005:intron 30.0%
GGTAGAAAACACAAATCAAT+TGG - chr7.3:48555062-48555081 MS.gene49005:CDS 30.0%
TAACACCTTCAAGAACTTTA+TGG - chr7.3:48556042-48556061 MS.gene49005:intron 30.0%
TAACATTGTTGAAGGGAATA+TGG + chr7.3:48556704-48556723 None:intergenic 30.0%
TAATGTCTTTGTAGATTGGA+GGG + chr7.3:48557100-48557119 None:intergenic 30.0%
TCCCAAAAAATAAGGGATTT+GGG - chr7.3:48556269-48556288 MS.gene49005:intron 30.0%
TCTATCATAAGTGGAATTAG+TGG + chr7.3:48555094-48555113 None:intergenic 30.0%
TTACTTCTACAAGATCAACT+TGG - chr7.3:48556461-48556480 MS.gene49005:intron 30.0%
TTCCAAAAGATAAGGGATTT+GGG - chr7.3:48556209-48556228 MS.gene49005:intron 30.0%
TTCCCAAAAAATAAGGGATT+TGG - chr7.3:48556268-48556287 MS.gene49005:intron 30.0%
! ATGGTAATGTCTTTGTAGAT+TGG + chr7.3:48557104-48557123 None:intergenic 30.0%
! CCATTTGAAATATTCCCAAA+AGG - chr7.3:48556169-48556188 MS.gene49005:intron 30.0%
! GTCTTGTTAACATTGTTGAA+GGG + chr7.3:48556711-48556730 None:intergenic 30.0%
! TTCAGCTTTTCTATCATAAG+TGG + chr7.3:48555103-48555122 None:intergenic 30.0%
! TTTATCACACTATCCCTTTT+GGG + chr7.3:48556186-48556205 None:intergenic 30.0%
! TTTTATCACACTATCCCTTT+TGG + chr7.3:48556187-48556206 None:intergenic 30.0%
!! ATGTCTTTGAGGTCTATAAT+GGG + chr7.3:48555261-48555280 None:intergenic 30.0%
!! CCTTTTGGGAATATTTCAAA+TGG + chr7.3:48556172-48556191 None:intergenic 30.0%
!! GCTTTTGATGATTCAAAAGT+TGG - chr7.3:48555129-48555148 MS.gene49005:CDS 30.0%
!! TATGTCTTTGAGGTCTATAA+TGG + chr7.3:48555262-48555281 None:intergenic 30.0%
!! TTTGCTATTTGCCTAATACA+AGG + chr7.3:48555772-48555791 None:intergenic 30.0%
ACCTTTGATGTACCTTCAAT+TGG + chr7.3:48557041-48557060 None:intergenic 35.0%
ATCAATGTTCCTCCTATACA+TGG - chr7.3:48556515-48556534 MS.gene49005:intron 35.0%
CAAGTCCATAAAGTTCTTGA+AGG + chr7.3:48556050-48556069 None:intergenic 35.0%
CAATGTTCTGTGATAAGACT+CGG + chr7.3:48556974-48556993 None:intergenic 35.0%
CACAACAATTCAACTCTTTG+AGG + chr7.3:48556346-48556365 None:intergenic 35.0%
CATATGGATGGTAGTTCATT+TGG + chr7.3:48555573-48555592 None:intergenic 35.0%
CCTGAACCTGAATTAACAAT+GGG - chr7.3:48556404-48556423 MS.gene49005:intron 35.0%
GAAGCTACTATCAGTATGTT+TGG + chr7.3:48556435-48556454 None:intergenic 35.0%
GATTGATGTGTATGTCTTTG+AGG + chr7.3:48555272-48555291 None:intergenic 35.0%
GTAATGTCTTTGTAGATTGG+AGG + chr7.3:48557101-48557120 None:intergenic 35.0%
GTGAAAAATACGTGGAATCT+TGG + chr7.3:48555184-48555203 None:intergenic 35.0%
GTTAATTACCTGCATATGGA+TGG + chr7.3:48555585-48555604 None:intergenic 35.0%
TATTATGCAAAAGGCCTAGA+TGG - chr7.3:48557143-48557162 MS.gene49005:CDS 35.0%
TATTTGCCTAATACAAGGAG+TGG + chr7.3:48555767-48555786 None:intergenic 35.0%
TCCTGAACCTGAATTAACAA+TGG - chr7.3:48556403-48556422 MS.gene49005:intron 35.0%
TCGCGAAACATTCGTAATAA+TGG - chr7.3:48555813-48555832 MS.gene49005:intron 35.0%
TGTGAAGGACTCTTTATTCA+AGG - chr7.3:48556362-48556381 MS.gene49005:intron 35.0%
! AACCCAAATCCCTTATCTTT+TGG + chr7.3:48556214-48556233 None:intergenic 35.0%
! ACCAATTGAAGGTACATCAA+AGG - chr7.3:48557037-48557056 MS.gene49005:CDS 35.0%
! ATTCCACGTATTTTTCACAC+CGG - chr7.3:48555186-48555205 MS.gene49005:CDS 35.0%
! GAAGGTACATCAAAGGTTTA+TGG - chr7.3:48557044-48557063 MS.gene49005:CDS 35.0%
! TATAATGGGAACACTCAACT+TGG + chr7.3:48555247-48555266 None:intergenic 35.0%
! TATTTTTCACACCGGAAAGT+TGG - chr7.3:48555194-48555213 MS.gene49005:CDS 35.0%
! TGAGTTTTCGCTGTAGTTTT+AGG - chr7.3:48555641-48555660 MS.gene49005:intron 35.0%
! TGATGGGCAAGAACATAATT+AGG + chr7.3:48555613-48555632 None:intergenic 35.0%
!! GGTAGTTCATTTGGTTTGAA+TGG + chr7.3:48555564-48555583 None:intergenic 35.0%
!! GGTCTTGTTAACATTGTTGA+AGG + chr7.3:48556712-48556731 None:intergenic 35.0%
!! TCATTTGGTTTGAATGGATG+AGG + chr7.3:48555558-48555577 None:intergenic 35.0%
!! TCTTGAAGGTGTTACTTCAT+CGG + chr7.3:48556036-48556055 None:intergenic 35.0%
!!! AGCCCAAATCCCTTATTTTT+TGG + chr7.3:48556274-48556293 None:intergenic 35.0%
!!! GCCCAAATCCCTTATTTTTT+GGG + chr7.3:48556273-48556292 None:intergenic 35.0%
AAACGTAGGAGATCTTCTAC+AGG - chr7.3:48556550-48556569 MS.gene49005:intron 40.0%
ATGAACTACCATCCATATGC+AGG - chr7.3:48555574-48555593 MS.gene49005:intron 40.0%
CCAAGAAAGAGTTTCCATCT+AGG + chr7.3:48557160-48557179 None:intergenic 40.0%
CCTAGATGGAAACTCTTTCT+TGG - chr7.3:48557157-48557176 MS.gene49005:CDS 40.0%
CTTCTACAAGATCAACTTGG+TGG - chr7.3:48556464-48556483 MS.gene49005:intron 40.0%
GAACATTGGCCCAAGAATTT+CGG - chr7.3:48556986-48557005 MS.gene49005:CDS 40.0%
GCTAATTGGAGAGATACACT+TGG - chr7.3:48555519-48555538 MS.gene49005:intron 40.0%
TATCTCTCCAATTAGCAGCT+TGG + chr7.3:48555515-48555534 None:intergenic 40.0%
TCTGAAAGGAGCTCCTTAAT+AGG + chr7.3:48557071-48557090 None:intergenic 40.0%
TGCAGCTTATCTCAAATGAC+AGG - chr7.3:48556931-48556950 MS.gene49005:CDS 40.0%
TTCAGTGATCTCCAACTTTC+CGG + chr7.3:48555208-48555227 None:intergenic 40.0%
TTTCCACCACTCCTTGTATT+AGG - chr7.3:48555758-48555777 MS.gene49005:intron 40.0%
TTTCCGGTGTGAAAAATACG+TGG + chr7.3:48555192-48555211 None:intergenic 40.0%
TTTGAATGGATGAGGAGCAA+CGG + chr7.3:48555550-48555569 None:intergenic 40.0%
! CCCATTGTTAATTCAGGTTC+AGG + chr7.3:48556407-48556426 None:intergenic 40.0%
! CGAGTCTTATCACAGAACAT+TGG - chr7.3:48556972-48556991 MS.gene49005:CDS 40.0%
! GTTAATTCAGGTTCAGGACA+TGG + chr7.3:48556401-48556420 None:intergenic 40.0%
! TCCAATGGAACCTTGTTTAG+CGG - chr7.3:48555489-48555508 MS.gene49005:intron 40.0%
! TGGTCTTCAAGTTCTTCATG+AGG - chr7.3:48556484-48556503 MS.gene49005:intron 40.0%
! TTGGAGGGTTTTCTTCTGAA+AGG + chr7.3:48557085-48557104 None:intergenic 40.0%
!! AAAAGTTGGTGTCAGAGGAT+TGG - chr7.3:48555143-48555162 MS.gene49005:CDS 40.0%
!! AAGCTTGCTACCGAAATTCT+TGG + chr7.3:48556999-48557018 None:intergenic 40.0%
!! AGCTTGCTACCGAAATTCTT+GGG + chr7.3:48556998-48557017 None:intergenic 40.0%
!! GAATTATTGTCGGAGGCATT+AGG - chr7.3:48556305-48556324 MS.gene49005:intron 40.0%
!! GATTCAAAAGTTGGTGTCAG+AGG - chr7.3:48555138-48555157 MS.gene49005:CDS 40.0%
ACAAGAGCTCCATGTATAGG+AGG + chr7.3:48556527-48556546 None:intergenic 45.0%
ACCACAAGAGCTCCATGTAT+AGG + chr7.3:48556530-48556549 None:intergenic 45.0%
ATCACCTCTATACGTAGAGC+TGG + chr7.3:48555294-48555313 None:intergenic 45.0%
CAATCCAGCTCTACGTATAG+AGG - chr7.3:48555287-48555306 MS.gene49005:intron 45.0%
CCTGAAAAAATCCCCACTCA+TGG + chr7.3:48555338-48555357 None:intergenic 45.0%
GCAGTGCCCATTGTTAATTC+AGG + chr7.3:48556413-48556432 None:intergenic 45.0%
TCAGTAAGTGCCACATGTGT+CGG + chr7.3:48555709-48555728 None:intergenic 45.0%
TCCTATACATGGAGCTCTTG+TGG - chr7.3:48556526-48556545 MS.gene49005:intron 45.0%
TTGCCTAATACAAGGAGTGG+TGG + chr7.3:48555764-48555783 None:intergenic 45.0%
! AATTCAGGTTCAGGACATGG+TGG + chr7.3:48556398-48556417 None:intergenic 45.0%
! TCAGAGGATTGGTAGAGTCT+GGG - chr7.3:48555154-48555173 MS.gene49005:CDS 45.0%
! TGTGGCACTTACTGATTGCA+TGG - chr7.3:48555714-48555733 MS.gene49005:intron 45.0%
!!! CCATGAGTGGGGATTTTTTC+AGG - chr7.3:48555335-48555354 MS.gene49005:intron 45.0%
AAATCCCCACTCATGGCATG+CGG + chr7.3:48555331-48555350 None:intergenic 50.0%
AAGGGAATATGGCCGTCTTG+AGG + chr7.3:48556693-48556712 None:intergenic 50.0%
ACATGTGTCGGGAAATGCTC+GGG + chr7.3:48555697-48555716 None:intergenic 50.0%
CACATGTGTCGGGAAATGCT+CGG + chr7.3:48555698-48555717 None:intergenic 50.0%
CAGTAAGTGCCACATGTGTC+GGG + chr7.3:48555708-48555727 None:intergenic 50.0%
CATGGCATGCGGTTCGAATT+TGG + chr7.3:48555320-48555339 None:intergenic 50.0%
GCCGCTAAACAAGGTTCCAT+TGG + chr7.3:48555493-48555512 None:intergenic 50.0%
GGAGCTCTTGTGGTAAACGT+AGG - chr7.3:48556536-48556555 MS.gene49005:intron 50.0%
TTAGCGGCCAAGCTGCTAAT+TGG - chr7.3:48555505-48555524 MS.gene49005:intron 50.0%
! CAGAGGATTGGTAGAGTCTG+GGG - chr7.3:48555155-48555174 MS.gene49005:CDS 50.0%
! GTCAGAGGATTGGTAGAGTC+TGG - chr7.3:48555153-48555172 MS.gene49005:CDS 50.0%
AGCAGCTTGGCCGCTAAACA+AGG + chr7.3:48555502-48555521 None:intergenic 55.0%
CGAGCATTTCCCGACACATG+TGG - chr7.3:48555696-48555715 MS.gene49005:intron 55.0%
TCGAACCGCATGCCATGAGT+GGG - chr7.3:48555323-48555342 MS.gene49005:intron 55.0%
TTCGAACCGCATGCCATGAG+TGG - chr7.3:48555322-48555341 MS.gene49005:intron 55.0%
CGAACCGCATGCCATGAGTG+GGG - chr7.3:48555324-48555343 MS.gene49005:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 48555057 48557196 48555057 ID=MS.gene49005
chr7.3 mRNA 48555057 48557196 48555057 ID=MS.gene49005.t1;Parent=MS.gene49005
chr7.3 exon 48556658 48557196 48556658 ID=MS.gene49005.t1.exon1;Parent=MS.gene49005.t1
chr7.3 CDS 48556658 48557196 48556658 ID=cds.MS.gene49005.t1;Parent=MS.gene49005.t1
chr7.3 exon 48555057 48555300 48555057 ID=MS.gene49005.t1.exon2;Parent=MS.gene49005.t1
chr7.3 CDS 48555057 48555300 48555057 ID=cds.MS.gene49005.t1;Parent=MS.gene49005.t1
Gene Sequence

>MS.gene49005

ATGGTGGTAGAAAACACAAATCAATTGGAGGAATCCACTAATTCCACTTATGATAGAAAAGCTGAAGTGAAAGCTTTTGATGATTCAAAAGTTGGTGTCAGAGGATTGGTAGAGTCTGGGGTGTCCAAGATTCCACGTATTTTTCACACCGGAAAGTTGGAGATCACTGAAAACTCAGATATTGTCTCCAAGTTGAGTGTTCCCATTATAGACCTCAAAGACATACACATCAATCCAGCTCTACGTATAGAGGTGATAGACCAAATTCGAACCGCATGCCATGAGTGGGGATTTTTTCAGGTGATTAATCACGAAATTTCCACTATTATCTTGGATGAAATGATTGATGGAATCGGTAAGTTTCATGAACAAGATATTGATGTAAGAAAAGAGTTTTACACTCGTGATTTAAAGAAAAAAGTTATGTATTTCTCCAATGGAACCTTGTTTAGCGGCCAAGCTGCTAATTGGAGAGATACACTTGGTTTCGCCGTTGCTCCTCATCCATTCAAACCAAATGAACTACCATCCATATGCAGGTAATTAACTAAGTCCTAATTATGTTCTTGCCCATCAATTTATAATGAGTTTTCGCTGTAGTTTTAGGAAAATGCTATTTCAAGGGAATGATATGCTTCCCGAGCATTTCCCGACACATGTGGCACTTACTGATTGCATGGTCAATTAGTCACTTGTTGTCTTTTCCACCACTCCTTGTATTAGGCAAATAGCAAATTTTGATTGTGCATGTTTAAGTCGCGAAACATTCGTAATAATGGTTTTCGCTTTATATAGCATTACATGTTGATTTATTATGTTTAATTTTATTATGTTTTTATTTATTAATTCCATTATACATTCATGAGTCTTCTCACCTAACTACGAGTATAATTTGAAGGAAAAAAACTCGGTTTTTATTTTATTTTACAATATCAATAGTTTTGAAACGAAAAATCAAACATTAATAGATATGGAACCGATGAAGTAACACCTTCAAGAACTTTATGGACTTGCATTGCAGCACTAATTATGACATATGATACAATCAAGTTAACTAGATTTCACATTTGTTACGATACATGACATATGATCATTTTAGCCATATATCATGTCCATTTGAAATATTCCCAAAAGGGATAGTGTGATAAAATATTCCAAAAGATAAGGGATTTGGGTTTCTTACATGTTTGCAGAGATAGTGTGATAAAATATTCCCAAAAAATAAGGGATTTGGGCTTCATAATCTTTGAATTATTGTCGGAGGCATTAGGACTTGATCGTGATTACCTCAAAGAGTTGAATTGTTGTGAAGGACTCTTTATTCAAGGTCATTACTATCCACCATGTCCTGAACCTGAATTAACAATGGGCACTGCCAAACATACTGATAGTAGCTTCATAACATTACTTCTACAAGATCAACTTGGTGGTCTTCAAGTTCTTCATGAGGATAAATGGATCAATGTTCCTCCTATACATGGAGCTCTTGTGGTAAACGTAGGAGATCTTCTACAGGTAAATATTACTCCCTTTGTCATTGTTTAAGTCAATGATTTGAAGTTATGCATAATCTTTTGGATAGTCTGTTTTCTTGCTATTTTCATTCAACAATGTTCTTCAGAATTATAATTGTCCTCAAGACGGCCATATTCCCTTCAACAATGTTAACAAGACCCTTACAAAAAAATATAACTGATCAATTTTCACATCATTTGTATTTTCATAGTACCATTGCATATTAGTATATTACTATCAATATTTAATTATAGTGAACTGCAATTATGGGCTAGAAATCATAATAATAAAAAAAAATCATTGATTTCTTCTTCAATGAAAGTTTGTGAGAATAATTTATCATTTTCTTTTTGTACTATTGCAGCTTATCTCAAATGACAGGTTTGTAAGTGTTTATCACCGAGTCTTATCACAGAACATTGGCCCAAGAATTTCGGTAGCAAGCTTCTTTGTGAATTCTCATGAACCAATTGAAGGTACATCAAAGGTTTATGGTCCTATTAAGGAGCTCCTTTCAGAAGAAAACCCTCCAATCTACAAAGACATTACCATAAAAGATTTCTTTGCACATTATTATGCAAAAGGCCTAGATGGAAACTCTTTCTTGGAGCCCTTCAAGTTGTGA

Protein sequence

>MS.gene49005.t1

MVVENTNQLEESTNSTYDRKAEVKAFDDSKVGVRGLVESGVSKIPRIFHTGKLEITENSDIVSKLSVPIIDLKDIHINPALRIEVIDQIRTACHEWGFFQVINHEISTIILDEMIDGIGKFHEQDIDVRKEFYTRDLKKKVMYFSNGTLFSGQAANWRDTLGFAVAPHPFKPNELPSICRFVSVYHRVLSQNIGPRISVASFFVNSHEPIEGTSKVYGPIKELLSEENPPIYKDITIKDFFAHYYAKGLDGNSFLEPFKL