Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49080.t1 | XP_013469679.1 | 68.6 | 121 | 38 | 0 | 25 | 145 | 89 | 209 | 9.60E-09 | 70.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49080.t1 | Q9FFA5 | 94.1 | 119 | 7 | 0 | 25 | 143 | 82 | 200 | 3.5e-07 | 56.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49080.t1 | G8A207 | 68.6 | 121 | 38 | 0 | 25 | 145 | 89 | 209 | 7.0e-09 | 70.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052056 | MS.gene49080 | -0.823917 | 1.07E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49080.t1 | MTR_1g100647 | 95.868 | 121 | 5 | 0 | 25 | 145 | 89 | 209 | 2.51e-76 | 226 |
| MS.gene49080.t1 | MTR_7g076730 | 67.742 | 124 | 40 | 0 | 22 | 145 | 10 | 133 | 1.60e-53 | 165 |
| MS.gene49080.t1 | MTR_8g031370 | 66.942 | 121 | 40 | 0 | 25 | 145 | 80 | 200 | 1.53e-52 | 165 |
| MS.gene49080.t1 | MTR_5g010590 | 61.157 | 121 | 47 | 0 | 25 | 145 | 87 | 207 | 8.57e-45 | 145 |
| MS.gene49080.t1 | MTR_8g097320 | 57.851 | 121 | 51 | 0 | 25 | 145 | 78 | 198 | 2.06e-44 | 144 |
| MS.gene49080.t1 | MTR_8g097320 | 57.851 | 121 | 51 | 0 | 25 | 145 | 81 | 201 | 2.34e-44 | 144 |
| MS.gene49080.t1 | MTR_5g010590 | 61.404 | 57 | 22 | 0 | 25 | 81 | 87 | 143 | 1.90e-15 | 70.1 |
| MS.gene49080.t1 | MTR_1g052560 | 35.043 | 117 | 76 | 0 | 24 | 140 | 153 | 269 | 1.81e-14 | 68.6 |
| MS.gene49080.t1 | MTR_1g019280 | 36.170 | 94 | 60 | 0 | 42 | 135 | 513 | 606 | 4.69e-13 | 65.5 |
| MS.gene49080.t1 | MTR_1g019280 | 36.170 | 94 | 60 | 0 | 42 | 135 | 451 | 544 | 1.94e-12 | 63.5 |
| MS.gene49080.t1 | MTR_7g091990 | 34.513 | 113 | 74 | 0 | 28 | 140 | 163 | 275 | 1.83e-11 | 60.5 |
| MS.gene49080.t1 | MTR_8g079482 | 38.532 | 109 | 64 | 1 | 30 | 138 | 322 | 427 | 5.34e-11 | 59.7 |
| MS.gene49080.t1 | MTR_8g079482 | 38.532 | 109 | 64 | 1 | 30 | 138 | 412 | 517 | 6.81e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49080.t1 | AT5G23750 | 78.512 | 121 | 26 | 0 | 25 | 145 | 81 | 201 | 3.55e-61 | 187 |
| MS.gene49080.t1 | AT5G23750 | 78.512 | 121 | 26 | 0 | 25 | 145 | 109 | 229 | 4.34e-61 | 188 |
| MS.gene49080.t1 | AT5G23750 | 78.512 | 121 | 26 | 0 | 25 | 145 | 82 | 202 | 5.73e-61 | 186 |
| MS.gene49080.t1 | AT3G48940 | 73.554 | 121 | 32 | 0 | 25 | 145 | 55 | 175 | 7.52e-59 | 180 |
| MS.gene49080.t1 | AT3G61260 | 68.595 | 121 | 38 | 0 | 25 | 145 | 92 | 212 | 2.04e-53 | 168 |
| MS.gene49080.t1 | AT2G45820 | 70.248 | 121 | 36 | 0 | 25 | 145 | 70 | 190 | 1.05e-47 | 152 |
| MS.gene49080.t1 | AT1G63295 | 43.056 | 144 | 74 | 4 | 8 | 145 | 10 | 151 | 7.74e-25 | 93.2 |
| MS.gene49080.t1 | AT1G63295 | 43.609 | 133 | 70 | 2 | 8 | 137 | 50 | 180 | 1.06e-24 | 94.0 |
| MS.gene49080.t1 | AT4G00670 | 42.157 | 102 | 59 | 0 | 39 | 140 | 16 | 117 | 7.44e-21 | 82.4 |
| MS.gene49080.t1 | AT3G57540 | 32.258 | 124 | 82 | 1 | 23 | 144 | 172 | 295 | 5.39e-12 | 62.0 |
| MS.gene49080.t1 | AT2G41870 | 33.884 | 121 | 79 | 1 | 26 | 145 | 153 | 273 | 1.20e-11 | 60.8 |
| MS.gene49080.t1 | AT1G30320 | 40.594 | 101 | 60 | 0 | 42 | 142 | 401 | 501 | 6.43e-11 | 59.3 |
Find 24 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGACCAAAGAGTTTCTT+TGG | 0.217523 | 1.3:-69513524 | None:intergenic |
| TCGATAGAGCCGCCCTCTTT+TGG | 0.297765 | 1.3:-69512843 | None:intergenic |
| GGAACAACTAGAGAAGAAAA+AGG | 0.300679 | 1.3:+69513355 | MS.gene49080:intron |
| ACTGTTCTCCCATGCTGTTA+TGG | 0.379485 | 1.3:-69513206 | None:intergenic |
| ACAGCTCCAAAGAAACTCTT+TGG | 0.398417 | 1.3:+69513518 | MS.gene49080:CDS |
| AAGAGGTAGCTGCAAAATAC+AGG | 0.425184 | 1.3:+69513486 | MS.gene49080:CDS |
| GCTGCAAAATACAGGGCAAC+TGG | 0.430234 | 1.3:+69513494 | MS.gene49080:CDS |
| CAAGGCAGCTGAAGAAAAGA+AGG | 0.521517 | 1.3:+69513421 | MS.gene49080:CDS |
| TTTCTCTTCAGTAACTTCAT+CGG | 0.524248 | 1.3:-69512820 | None:intergenic |
| GGCTGTCACTAATCAAAGCA+TGG | 0.531510 | 1.3:+69512992 | MS.gene49080:CDS |
| CAAGAGTTGCAACAGAAAAG+AGG | 0.549164 | 1.3:+69512971 | MS.gene49080:CDS |
| GCTATGATTGAGGCAAAGAA+AGG | 0.579579 | 1.3:+69513443 | MS.gene49080:CDS |
| AACTTTCATCCATAACAGCA+TGG | 0.579782 | 1.3:+69513197 | MS.gene49080:CDS |
| TACTGAAGAGAAACCAAAAG+AGG | 0.582288 | 1.3:+69512830 | MS.gene49080:CDS |
| AGAATTACTCAAAGCAGAAG+AGG | 0.586549 | 1.3:+69513469 | MS.gene49080:CDS |
| CAAAATAGCAGCACTTCACA+AGG | 0.592299 | 1.3:+69513403 | MS.gene49080:CDS |
| AGAAAAGAAGGCTATGATTG+AGG | 0.593916 | 1.3:+69513433 | MS.gene49080:CDS |
| AGCTGAATTGAGAAAACTTG+AGG | 0.599321 | 1.3:+69513246 | MS.gene49080:CDS |
| GCTGTCACTAATCAAAGCAT+GGG | 0.606143 | 1.3:+69512993 | MS.gene49080:CDS |
| ACTTTCATCCATAACAGCAT+GGG | 0.613867 | 1.3:+69513198 | MS.gene49080:CDS |
| AGAGGTAGCTGCAAAATACA+GGG | 0.626640 | 1.3:+69513487 | MS.gene49080:CDS |
| ACTGAAGAGAAACCAAAAGA+GGG | 0.650321 | 1.3:+69512831 | MS.gene49080:CDS |
| GAAGAGAAACCAAAAGAGGG+CGG | 0.701063 | 1.3:+69512834 | MS.gene49080:CDS |
| GAGGGCGGCTCTATCGACCG+TGG | 0.735676 | 1.3:+69512849 | MS.gene49080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATGCTTAATGTGTATTTT+TGG | + | chr1.3:69513065-69513084 | MS.gene49080:intron | 20.0% |
| ! | AATACACATTAAGCATTAGA+TGG | - | chr1.3:69513063-69513082 | None:intergenic | 25.0% |
| ! | ACATTGAATGTGACAAAATA+TGG | + | chr1.3:69513295-69513314 | MS.gene49080:intron | 25.0% |
| ! | ATTGAATGTGACAAAATATG+GGG | + | chr1.3:69513297-69513316 | MS.gene49080:intron | 25.0% |
| ! | CATTGAATGTGACAAAATAT+GGG | + | chr1.3:69513296-69513315 | MS.gene49080:intron | 25.0% |
| ! | TACTAATCTCCTTAGAATTA+TGG | - | chr1.3:69513128-69513147 | None:intergenic | 25.0% |
| !!! | AATTACTTTTCACTGTGAAT+AGG | + | chr1.3:69513334-69513353 | MS.gene49080:intron | 25.0% |
| AAAAGACCAAAGAGTTTCTT+TGG | - | chr1.3:69513527-69513546 | None:intergenic | 30.0% | |
| ACTTCATATGCTAACATTAC+AGG | + | chr1.3:69512912-69512931 | MS.gene49080:intron | 30.0% | |
| ATTGAGTCACCATAATTCTA+AGG | + | chr1.3:69513116-69513135 | MS.gene49080:intron | 30.0% | |
| CAATGACAAGATATATACCA+CGG | - | chr1.3:69512869-69512888 | None:intergenic | 30.0% | |
| GAACAACTAGAGAAGAAAAA+GGG | + | chr1.3:69513356-69513375 | MS.gene49080:CDS | 30.0% | |
| TAGCATATGAAGTGCATAAA+GGG | - | chr1.3:69512905-69512924 | None:intergenic | 30.0% | |
| TATGGTGACTCAATGTAATA+AGG | - | chr1.3:69513110-69513129 | None:intergenic | 30.0% | |
| TTAGCATATGAAGTGCATAA+AGG | - | chr1.3:69512906-69512925 | None:intergenic | 30.0% | |
| TTGAATGTGACAAAATATGG+GGG | + | chr1.3:69513298-69513317 | MS.gene49080:intron | 30.0% | |
| AACTTTCATCCATAACAGCA+TGG | + | chr1.3:69513197-69513216 | MS.gene49080:CDS | 35.0% | |
| ACTGAAGAGAAACCAAAAGA+GGG | + | chr1.3:69512831-69512850 | MS.gene49080:CDS | 35.0% | |
| ACTTTCATCCATAACAGCAT+GGG | + | chr1.3:69513198-69513217 | MS.gene49080:CDS | 35.0% | |
| AGAAAAGAAGGCTATGATTG+AGG | + | chr1.3:69513433-69513452 | MS.gene49080:CDS | 35.0% | |
| AGAATTACTCAAAGCAGAAG+AGG | + | chr1.3:69513469-69513488 | MS.gene49080:CDS | 35.0% | |
| AGCTGAATTGAGAAAACTTG+AGG | + | chr1.3:69513246-69513265 | MS.gene49080:CDS | 35.0% | |
| GGAACAACTAGAGAAGAAAA+AGG | + | chr1.3:69513355-69513374 | MS.gene49080:intron | 35.0% | |
| TACTGAAGAGAAACCAAAAG+AGG | + | chr1.3:69512830-69512849 | MS.gene49080:CDS | 35.0% | |
| AAGAGGTAGCTGCAAAATAC+AGG | + | chr1.3:69513486-69513505 | MS.gene49080:CDS | 40.0% | |
| ACAGCTCCAAAGAAACTCTT+TGG | + | chr1.3:69513518-69513537 | MS.gene49080:CDS | 40.0% | |
| AGAGGTAGCTGCAAAATACA+GGG | + | chr1.3:69513487-69513506 | MS.gene49080:CDS | 40.0% | |
| CAAAATAGCAGCACTTCACA+AGG | + | chr1.3:69513403-69513422 | MS.gene49080:CDS | 40.0% | |
| CAAGAGTTGCAACAGAAAAG+AGG | + | chr1.3:69512971-69512990 | MS.gene49080:CDS | 40.0% | |
| CATTAAGCATTAGATGGTGC+AGG | - | chr1.3:69513057-69513076 | None:intergenic | 40.0% | |
| GCTATGATTGAGGCAAAGAA+AGG | + | chr1.3:69513443-69513462 | MS.gene49080:CDS | 40.0% | |
| GCTGTCACTAATCAAAGCAT+GGG | + | chr1.3:69512993-69513012 | MS.gene49080:CDS | 40.0% | |
| ACTGTTCTCCCATGCTGTTA+TGG | - | chr1.3:69513209-69513228 | None:intergenic | 45.0% | |
| CAAGGCAGCTGAAGAAAAGA+AGG | + | chr1.3:69513421-69513440 | MS.gene49080:CDS | 45.0% | |
| GAAGAGAAACCAAAAGAGGG+CGG | + | chr1.3:69512834-69512853 | MS.gene49080:CDS | 45.0% | |
| GGCTGTCACTAATCAAAGCA+TGG | + | chr1.3:69512992-69513011 | MS.gene49080:CDS | 45.0% | |
| GCTGCAAAATACAGGGCAAC+TGG | + | chr1.3:69513494-69513513 | MS.gene49080:CDS | 50.0% | |
| ! | TCGATAGAGCCGCCCTCTTT+TGG | - | chr1.3:69512846-69512865 | None:intergenic | 55.0% |
| GAGGGCGGCTCTATCGACCG+TGG | + | chr1.3:69512849-69512868 | MS.gene49080:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 69512823 | 69513550 | 69512823 | ID=MS.gene49080 |
| chr1.3 | mRNA | 69512823 | 69513550 | 69512823 | ID=MS.gene49080.t1;Parent=MS.gene49080 |
| chr1.3 | exon | 69512823 | 69512870 | 69512823 | ID=MS.gene49080.t1.exon1;Parent=MS.gene49080.t1 |
| chr1.3 | CDS | 69512823 | 69512870 | 69512823 | ID=cds.MS.gene49080.t1;Parent=MS.gene49080.t1 |
| chr1.3 | exon | 69512934 | 69513046 | 69512934 | ID=MS.gene49080.t1.exon2;Parent=MS.gene49080.t1 |
| chr1.3 | CDS | 69512934 | 69513046 | 69512934 | ID=cds.MS.gene49080.t1;Parent=MS.gene49080.t1 |
| chr1.3 | exon | 69513186 | 69513267 | 69513186 | ID=MS.gene49080.t1.exon3;Parent=MS.gene49080.t1 |
| chr1.3 | CDS | 69513186 | 69513267 | 69513186 | ID=cds.MS.gene49080.t1;Parent=MS.gene49080.t1 |
| chr1.3 | exon | 69513356 | 69513550 | 69513356 | ID=MS.gene49080.t1.exon4;Parent=MS.gene49080.t1 |
| chr1.3 | CDS | 69513356 | 69513550 | 69513356 | ID=cds.MS.gene49080.t1;Parent=MS.gene49080.t1 |
| Gene Sequence |
| Protein sequence |
