AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49190.t1 MTR_5g072570 95.106 470 23 0 1 470 1 470 0.0 942
MS.gene49190.t1 MTR_3g090130 77.778 468 99 2 1 468 1 463 0.0 768
MS.gene49190.t1 MTR_3g090150 75.316 474 107 4 1 468 1 470 0.0 745
MS.gene49190.t1 MTR_7g021130 47.926 434 217 6 30 461 31 457 8.26e-143 417
MS.gene49190.t1 MTR_8g099930 46.575 438 227 5 28 463 32 464 1.11e-140 412
MS.gene49190.t1 MTR_3g498755 45.909 440 225 7 28 461 13 445 3.32e-139 407
MS.gene49190.t1 MTR_3g434850 46.507 458 227 7 9 461 10 454 9.68e-139 407
MS.gene49190.t1 MTR_2g449850 45.413 436 225 6 31 463 39 464 1.60e-129 383
MS.gene49190.t1 MTR_7g013790 42.227 476 244 9 6 465 8 468 4.69e-124 369
MS.gene49190.t1 MTR_3g034290 44.295 447 225 8 23 465 68 494 1.99e-122 366
MS.gene49190.t1 MTR_4g089155 42.826 460 233 11 19 463 43 487 1.56e-120 361
MS.gene49190.t1 MTR_4g075150 42.398 467 245 11 7 461 9 463 1.76e-119 358
MS.gene49190.t1 MTR_4g075150 42.672 464 246 11 7 461 9 461 2.28e-119 357
MS.gene49190.t1 MTR_3g079570 45.330 439 214 10 26 461 75 490 5.15e-119 357
MS.gene49190.t1 MTR_2g012860 43.049 446 232 7 28 462 40 474 6.80e-119 357
MS.gene49190.t1 MTR_3g079590 45.161 434 215 9 26 457 74 486 4.15e-118 355
MS.gene49190.t1 MTR_0003s0560 43.991 441 227 7 22 461 66 487 5.34e-118 355
MS.gene49190.t1 MTR_3g079560 43.820 445 225 9 22 463 70 492 9.24e-116 349
MS.gene49190.t1 MTR_2g012850 41.964 448 236 8 27 463 33 467 1.31e-115 348
MS.gene49190.t1 MTR_1g040370 40.909 440 203 6 28 462 29 416 2.84e-115 345
MS.gene49190.t1 MTR_3g079600 44.222 450 226 12 22 467 68 496 3.63e-114 345
MS.gene49190.t1 MTR_1g095560 40.085 469 261 9 4 464 3 459 6.66e-112 338
MS.gene49190.t1 MTR_3g079620 42.070 454 226 12 26 467 74 502 1.29e-111 339
MS.gene49190.t1 MTR_1g021010 43.707 437 217 11 30 462 76 487 2.03e-110 335
MS.gene49190.t1 MTR_3g079630 42.325 456 223 12 26 467 74 503 9.80e-110 334
MS.gene49190.t1 MTR_2g081620 38.462 468 268 9 9 468 15 470 2.55e-109 332
MS.gene49190.t1 MTR_1g020090 43.311 441 214 11 27 461 87 497 4.98e-109 332
MS.gene49190.t1 MTR_4g122950 38.351 485 254 12 9 464 12 480 5.06e-108 329
MS.gene49190.t1 MTR_2g062990 40.278 432 243 9 33 461 43 462 1.56e-107 327
MS.gene49190.t1 MTR_2g063000 37.527 469 272 10 2 461 7 463 1.94e-107 327
MS.gene49190.t1 MTR_1g021020 43.607 438 219 10 28 462 76 488 4.35e-107 327
MS.gene49190.t1 MTR_1g095540 40.363 441 247 8 27 463 30 458 2.71e-105 321
MS.gene49190.t1 MTR_1g095560 37.525 501 261 10 4 464 3 491 6.75e-105 321
MS.gene49190.t1 MTR_4g122960 37.552 482 260 13 9 464 12 478 2.48e-103 317
MS.gene49190.t1 MTR_2g062980 39.492 433 245 9 33 461 43 462 5.94e-103 315
MS.gene49190.t1 MTR_3g034290 43.978 357 180 7 23 375 68 408 2.16e-95 294
MS.gene49190.t1 MTR_1g102130 38.565 446 228 12 28 466 90 496 1.41e-93 292
MS.gene49190.t1 MTR_2g063000 33.476 466 264 11 2 461 7 432 7.82e-87 273
MS.gene49190.t1 MTR_0003s0560 48.120 266 129 4 22 286 66 323 2.41e-80 253
MS.gene49190.t1 MTR_7g051430 32.016 506 281 13 1 457 1 492 3.67e-77 249
MS.gene49190.t1 MTR_8g011350 31.590 497 277 13 9 457 11 492 1.69e-71 234
MS.gene49190.t1 MTR_7g080180 31.755 507 282 17 6 460 4 498 1.63e-70 232
MS.gene49190.t1 MTR_8g011350 31.078 473 263 13 9 433 11 468 1.82e-63 213
MS.gene49190.t1 MTR_0075s0010 34.015 391 182 11 26 409 4 325 5.20e-59 197
MS.gene49190.t1 MTR_7g080180 31.183 465 256 17 6 418 4 456 1.63e-58 199
MS.gene49190.t1 MTR_7g051430 37.458 299 173 7 1 293 1 291 6.96e-56 191
MS.gene49190.t1 MTR_2g018630 29.374 463 285 15 18 460 25 465 4.10e-52 182
MS.gene49190.t1 MTR_2g018600 27.706 462 301 14 18 460 22 469 1.19e-49 176
MS.gene49190.t1 MTR_2g018590 28.000 475 305 17 6 460 12 469 9.16e-49 174
MS.gene49190.t1 MTR_4g021510 29.019 479 280 19 6 456 19 465 5.83e-48 172
MS.gene49190.t1 MTR_4g021535 27.579 475 300 18 2 456 12 462 2.45e-46 167
MS.gene49190.t1 MTR_4g021520 27.354 446 286 15 30 456 40 466 9.57e-46 166
MS.gene49190.t1 MTR_4g021490 27.088 443 291 15 30 456 40 466 1.61e-45 165
MS.gene49190.t1 MTR_1g050545 30.093 432 255 13 41 457 81 480 4.24e-45 164
MS.gene49190.t1 MTR_4g021530 26.398 447 289 16 30 456 40 466 4.43e-44 161
MS.gene49190.t1 MTR_4g057585 27.335 439 270 12 35 457 87 492 1.19e-43 160
MS.gene49190.t1 MTR_4g057595 27.455 448 272 13 37 468 90 500 3.20e-43 159
MS.gene49190.t1 MTR_2g095750 27.700 426 281 12 40 460 97 500 7.87e-43 159
MS.gene49190.t1 MTR_3g100420 27.826 460 290 18 28 460 52 496 1.64e-42 157
MS.gene49190.t1 MTR_4g021500 27.477 444 284 18 30 454 40 464 2.44e-42 160
MS.gene49190.t1 MTR_4g021500 26.196 439 287 16 36 456 482 901 2.90e-37 145
MS.gene49190.t1 MTR_4g021500 27.477 444 284 18 30 454 40 464 2.88e-42 160
MS.gene49190.t1 MTR_4g021500 26.107 429 282 15 36 456 482 883 9.08e-36 141
MS.gene49190.t1 MTR_2g095730 26.897 435 272 13 40 458 91 495 2.47e-40 152
MS.gene49190.t1 MTR_1g094155 27.315 432 268 12 40 457 87 486 4.61e-40 150
MS.gene49190.t1 MTR_8g007520 91.549 71 6 0 346 416 111 181 1.72e-38 138
MS.gene49190.t1 MTR_4g057570 27.400 427 278 13 41 458 87 490 2.58e-38 145
MS.gene49190.t1 MTR_3g045770 25.114 438 301 12 29 454 26 448 5.15e-37 141
MS.gene49190.t1 MTR_4g057600 27.397 438 260 13 41 458 93 492 1.07e-36 141
MS.gene49190.t1 MTR_4g021520 26.961 408 260 15 30 418 40 428 2.01e-35 136
MS.gene49190.t1 MTR_2g018630 28.453 362 224 12 113 460 20 360 7.26e-35 134
MS.gene49190.t1 MTR_5g097180 25.829 422 270 12 45 455 43 432 5.94e-32 127
MS.gene49190.t1 MTR_2g009200 25.828 453 269 16 28 456 26 435 6.33e-32 127
MS.gene49190.t1 MTR_4g021535 25.474 475 263 19 2 456 12 415 6.59e-32 126
MS.gene49190.t1 MTR_4g084710 24.886 438 270 15 37 455 37 434 7.18e-31 124
MS.gene49190.t1 MTR_2g095710 26.018 442 269 16 34 460 85 483 1.23e-26 112
MS.gene49190.t1 MTR_1g051175 52.941 85 37 2 37 120 20 102 4.61e-21 93.2
MS.gene49190.t1 MTR_7g100560 27.962 211 86 3 36 246 65 209 1.60e-19 89.7
MS.gene49190.t1 MTR_4g056520 21.957 460 277 17 42 456 32 454 2.51e-17 84.7
MS.gene49190.t1 MTR_6g086380 74.419 43 11 0 422 464 58 100 1.13e-14 70.1
MS.gene49190.t1 MTR_2g102960 82.857 35 6 0 346 380 36 70 1.34e-11 63.2
MS.gene49190.t1 MTR_2g084530 32.105 190 108 6 46 230 36 209 2.68e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49190.t1 AT5G42240 78.889 450 95 0 20 469 23 472 0.0 772
MS.gene49190.t1 AT1G43780 69.198 474 139 3 2 468 4 477 0.0 715
MS.gene49190.t1 AT5G42230 71.239 452 130 0 13 464 12 463 0.0 697
MS.gene49190.t1 AT5G42230 71.239 452 130 0 13 464 12 463 0.0 697
MS.gene49190.t1 AT2G12480 71.491 456 122 3 7 462 11 458 0.0 696
MS.gene49190.t1 AT2G12480 67.763 456 115 4 7 462 11 434 0.0 644
MS.gene49190.t1 AT2G12480 67.325 456 115 4 7 462 11 432 0.0 640
MS.gene49190.t1 AT2G12480 67.177 457 115 5 7 462 11 433 0.0 635
MS.gene49190.t1 AT2G33530 48.858 438 215 6 28 462 32 463 1.65e-148 432
MS.gene49190.t1 AT1G28110 48.064 439 215 7 28 462 30 459 8.29e-143 417
MS.gene49190.t1 AT1G28110 48.064 439 215 7 28 462 30 459 8.29e-143 417
MS.gene49190.t1 AT3G02110 44.222 450 223 8 28 462 36 472 4.04e-121 362
MS.gene49190.t1 AT3G63470 43.146 445 235 9 22 465 74 501 1.91e-119 359
MS.gene49190.t1 AT2G24010 42.986 442 231 8 27 463 28 453 4.48e-119 356
MS.gene49190.t1 AT4G30610 42.729 447 234 9 27 463 30 464 2.45e-118 355
MS.gene49190.t1 AT2G24000 42.247 445 234 8 27 460 28 460 1.33e-116 350
MS.gene49190.t1 AT4G30810 41.667 456 250 8 9 460 18 461 7.36e-116 349
MS.gene49190.t1 AT5G08260 39.917 481 256 10 5 462 7 477 1.67e-115 348
MS.gene49190.t1 AT5G23210 40.959 459 233 10 28 461 50 495 1.45e-114 346
MS.gene49190.t1 AT2G24010 41.357 457 232 9 27 463 28 468 1.14e-113 343
MS.gene49190.t1 AT1G61130 41.319 455 245 9 15 457 12 456 1.30e-112 340
MS.gene49190.t1 AT2G35770 42.664 443 233 11 26 463 34 460 3.12e-112 339
MS.gene49190.t1 AT2G35780 41.591 440 237 9 27 461 27 451 4.19e-111 336
MS.gene49190.t1 AT1G11080 40.798 451 234 9 27 457 48 485 9.85e-111 336
MS.gene49190.t1 AT3G07990 41.176 442 244 9 26 463 30 459 1.06e-110 335
MS.gene49190.t1 AT1G11080 40.798 451 234 9 27 457 48 485 1.21e-110 336
MS.gene49190.t1 AT2G24010 38.958 480 232 10 29 463 1 464 8.95e-107 325
MS.gene49190.t1 AT4G15100 39.167 480 259 9 5 463 16 483 1.33e-105 323
MS.gene49190.t1 AT2G05850 39.865 444 242 8 27 463 61 486 5.25e-104 319
MS.gene49190.t1 AT3G52020 40.271 442 241 12 27 463 77 500 5.74e-102 314
MS.gene49190.t1 AT3G17180 39.093 463 249 11 22 463 27 477 1.69e-101 312
MS.gene49190.t1 AT3G52000 38.776 441 246 9 27 463 61 481 2.08e-100 309
MS.gene49190.t1 AT5G23210 40.097 414 211 9 28 416 50 451 5.93e-98 302
MS.gene49190.t1 AT3G52010 38.514 444 247 10 27 463 62 486 3.72e-97 301
MS.gene49190.t1 AT5G23210 38.386 409 214 10 78 461 5 400 1.80e-89 278
MS.gene49190.t1 AT5G23210 38.272 405 212 10 82 461 8 399 2.95e-88 275
MS.gene49190.t1 AT2G24010 40.054 367 189 7 29 375 1 356 1.59e-84 264
MS.gene49190.t1 AT3G17180 37.260 416 228 11 22 416 27 430 3.50e-82 261
MS.gene49190.t1 AT4G12910 32.220 509 281 16 1 460 4 497 9.88e-76 246
MS.gene49190.t1 AT5G23210 36.944 360 190 9 82 416 8 355 1.36e-71 231
MS.gene49190.t1 AT3G25420 31.224 490 281 11 16 460 16 494 3.92e-69 228
MS.gene49190.t1 AT4G12910 31.478 467 256 16 1 418 4 455 3.05e-62 209
MS.gene49190.t1 AT3G56540 50.802 187 89 3 7 192 57 241 1.43e-55 186
MS.gene49190.t1 AT2G24000 35.880 301 171 7 170 460 109 397 3.76e-51 179
MS.gene49190.t1 AT2G24000 55.556 90 39 1 27 115 28 117 1.35e-25 108
MS.gene49190.t1 AT1G73300 29.213 445 267 15 29 460 32 441 4.71e-51 179
MS.gene49190.t1 AT1G73300 29.213 445 267 15 29 460 32 441 4.71e-51 179
MS.gene49190.t1 AT1G33540 28.062 449 294 14 29 460 28 464 9.29e-51 179
MS.gene49190.t1 AT1G33540 28.140 430 281 13 47 460 4 421 8.27e-50 176
MS.gene49190.t1 AT2G22920 28.475 446 271 16 29 460 24 435 1.18e-49 176
MS.gene49190.t1 AT1G73270 28.733 442 273 15 29 460 32 441 4.13e-49 174
MS.gene49190.t1 AT1G73270 28.733 442 273 15 29 460 32 441 5.86e-49 174
MS.gene49190.t1 AT5G09640 28.571 462 278 16 29 460 26 465 6.06e-49 174
MS.gene49190.t1 AT2G22980 27.803 446 268 15 29 460 25 430 6.96e-49 173
MS.gene49190.t1 AT5G36180 28.090 445 272 14 29 460 32 441 7.75e-49 173
MS.gene49190.t1 AT1G73280 29.140 477 285 18 1 460 1 441 7.91e-49 173
MS.gene49190.t1 AT1G73290 28.054 442 274 14 29 460 31 438 2.17e-47 169
MS.gene49190.t1 AT1G73310 27.314 443 278 14 29 460 32 441 4.23e-47 169
MS.gene49190.t1 AT2G23000 27.828 442 281 15 29 460 24 437 4.42e-47 169
MS.gene49190.t1 AT2G23000 27.828 442 281 15 29 460 24 437 4.42e-47 169
MS.gene49190.t1 AT2G23010 28.541 466 287 17 10 460 3 437 5.10e-47 168
MS.gene49190.t1 AT2G22990 27.578 446 275 16 29 460 22 433 1.19e-46 167
MS.gene49190.t1 AT2G23010 28.326 466 288 17 10 460 3 437 1.68e-46 167
MS.gene49190.t1 AT3G12240 28.953 449 263 17 29 460 27 436 3.57e-46 166
MS.gene49190.t1 AT2G22970 28.153 444 273 15 29 460 24 433 4.91e-46 166
MS.gene49190.t1 AT5G22980 28.505 428 269 12 40 458 95 494 2.01e-45 166
MS.gene49190.t1 AT2G22980 28.029 421 250 14 29 435 25 406 2.35e-45 163
MS.gene49190.t1 AT3G45010 28.924 446 275 13 35 467 92 508 4.27e-45 165
MS.gene49190.t1 AT2G22980 28.095 420 249 14 29 434 25 405 5.43e-45 163
MS.gene49190.t1 AT3G12230 28.571 448 266 16 29 460 26 435 5.53e-45 163
MS.gene49190.t1 AT3G10450 28.090 445 272 15 29 460 28 437 9.60e-45 162
MS.gene49190.t1 AT3G12220 28.027 446 271 16 29 460 26 435 5.00e-44 160
MS.gene49190.t1 AT3G12203 27.419 434 280 11 33 460 12 416 1.36e-43 159
MS.gene49190.t1 AT3G12203 26.991 452 295 11 15 460 15 437 1.43e-43 159
MS.gene49190.t1 AT3G10450 27.700 426 261 14 47 460 4 394 7.67e-43 156
MS.gene49190.t1 AT3G12220 28.103 427 258 15 47 460 15 405 1.54e-42 155
MS.gene49190.t1 AT2G22990 27.619 420 257 15 29 434 22 408 4.86e-42 154
MS.gene49190.t1 AT2G22970 28.162 419 256 14 29 435 24 409 6.09e-42 154
MS.gene49190.t1 AT2G22990 27.553 421 258 15 29 435 22 409 7.54e-42 155
MS.gene49190.t1 AT2G22970 28.230 418 255 14 29 434 24 408 2.32e-41 153
MS.gene49190.t1 AT3G12203 27.451 408 262 10 58 460 1 379 3.42e-41 151
MS.gene49190.t1 AT2G22990 26.906 446 269 17 29 460 22 424 1.60e-40 150
MS.gene49190.t1 AT3G10450 28.095 420 255 14 29 435 28 413 1.96e-40 150
MS.gene49190.t1 AT2G22920 27.427 412 238 15 29 419 24 395 8.39e-40 148
MS.gene49190.t1 AT3G10410 27.703 444 275 13 35 464 90 501 2.65e-39 149
MS.gene49190.t1 AT3G10450 27.681 401 244 13 47 435 4 370 1.37e-38 144
MS.gene49190.t1 AT1G73310 26.368 402 253 13 29 419 32 401 4.25e-37 141
MS.gene49190.t1 AT1G73290 26.933 401 250 13 29 419 31 398 4.93e-37 140
MS.gene49190.t1 AT1G73280 31.438 299 183 10 1 286 1 290 6.27e-35 134
MS.gene49190.t1 AT2G22920 30.970 268 166 9 29 286 24 282 2.65e-34 131
MS.gene49190.t1 AT1G73290 32.743 226 137 6 29 244 31 251 1.85e-32 125
MS.gene49190.t1 AT2G22980 30.597 268 164 9 29 286 25 280 9.02e-32 125
MS.gene49190.t1 AT5G36180 26.331 357 222 11 111 460 17 339 1.13e-31 124
MS.gene49190.t1 AT5G36180 26.331 357 222 11 111 460 17 339 1.13e-31 124
MS.gene49190.t1 AT1G73280 27.171 357 219 12 111 460 17 339 1.42e-31 124
MS.gene49190.t1 AT2G22970 32.917 240 146 7 29 259 24 257 2.98e-31 122
MS.gene49190.t1 AT1G15000 35.912 181 109 3 37 214 29 205 4.99e-29 119
MS.gene49190.t1 AT1G73280 26.724 348 214 12 120 460 1 314 1.78e-28 115
MS.gene49190.t1 AT2G22990 25.641 351 220 13 118 460 2 319 4.48e-28 114
MS.gene49190.t1 AT2G22990 25.356 351 221 13 118 460 2 319 1.99e-27 112
MS.gene49190.t1 AT2G22980 25.767 326 196 11 118 435 2 289 4.18e-27 110
MS.gene49190.t1 AT5G36180 25.904 332 206 10 111 435 17 315 6.72e-27 110
MS.gene49190.t1 AT3G12220 30.734 218 136 6 29 236 26 238 1.68e-25 108
MS.gene49190.t1 AT5G22960 30.857 175 99 3 35 208 25 178 4.26e-24 99.8
MS.gene49190.t1 AT1G73310 24.841 314 200 10 111 419 17 299 2.04e-22 98.2
MS.gene49190.t1 AT5G36180 25.397 315 195 10 111 418 17 298 1.76e-21 95.9
MS.gene49190.t1 AT2G27920 26.792 265 149 9 42 284 33 274 2.03e-19 90.9
MS.gene49190.t1 AT2G22920 25.850 294 163 13 182 460 3 256 1.69e-17 82.4
MS.gene49190.t1 AT2G27920 27.461 193 117 6 83 273 4 175 1.31e-12 69.7

Find 112 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCTCACTCTTTGGCTTAA+TGG 0.090755 5.4:+61471267 MS.gene49190:CDS
GAAACTTCTCGAACCATTTC+AGG 0.181933 5.4:-61472440 None:intergenic
GAGCACCATATGATTATTTC+TGG 0.211487 5.4:+61473076 MS.gene49190:CDS
TTCAAAACCGTATTCCAGTA+TGG 0.267358 5.4:+61473884 MS.gene49190:CDS
AAAATAAACCCCTCACTCTT+TGG 0.284368 5.4:+61471259 MS.gene49190:CDS
GGAGGTGCCTTTACTGAATT+AGG 0.292107 5.4:+61471986 MS.gene49190:CDS
TCTCAGTTCTTGTTCAACTA+TGG 0.292611 5.4:-61473283 None:intergenic
GATCTGGTTGTTAGTCTTCC+TGG 0.309372 5.4:+61471132 MS.gene49190:CDS
GGAATGATTTCAGATGAAAT+TGG 0.326242 5.4:+61473105 MS.gene49190:CDS
TCCAGAGCGTTGTTTCTTAC+CGG 0.327019 5.4:+61472474 MS.gene49190:CDS
TATTGACTGAATTCAACTTT+AGG 0.341208 5.4:-61471159 None:intergenic
CTCTTTATTGAGTCTCCTGC+TGG 0.357375 5.4:+61472191 MS.gene49190:CDS
TGCTTTCGTTCTTCCTGAAA+TGG 0.362996 5.4:+61472427 MS.gene49190:CDS
GGAGGTTGGGCAACTGAGTA+TGG 0.366756 5.4:+61474473 MS.gene49190:CDS
GGACTGTAATCTTGAACTTC+AGG 0.367870 5.4:-61474197 None:intergenic
ATTGGAAGCATATTAATGTT+AGG 0.368350 5.4:-61473847 None:intergenic
TGAACAATCCGTTTGAGAAT+TGG 0.369737 5.4:-61473865 None:intergenic
GATTCCGTTGTGCCATTACT+AGG 0.370360 5.4:+61474141 MS.gene49190:CDS
AAATAATTACCTGGCCTTTG+TGG 0.380863 5.4:-61474236 None:intergenic
CTAACAACCAGATCCTCAAT+AGG 0.380884 5.4:-61471123 None:intergenic
CTCGTCACCGATGTTGTAAT+CGG 0.389457 5.4:-61472243 None:intergenic
TTTCAAATTGAACTTGTAAC+CGG 0.392444 5.4:-61472900 None:intergenic
AAAATTGATACCTCTATTCC+AGG 0.400614 5.4:-61472059 None:intergenic
GCTCTAGAAGGTTGTGCATA+TGG 0.402187 5.4:-61474542 None:intergenic
AAACAACGCTCTGGATTTGT+AGG 0.402525 5.4:-61472466 None:intergenic
CCTGGTTGTTCTTCCGTTGG+AGG 0.405009 5.4:+61471965 MS.gene49190:CDS
AGGGTACCCTATTGAGGATC+TGG 0.409525 5.4:+61471116 MS.gene49190:CDS
ACAACAAGACCTTCAATTGA+TGG 0.411072 5.4:+61474611 MS.gene49190:CDS
GTACCAACTTGCCGTATTCA+TGG 0.415219 5.4:+61473514 MS.gene49190:CDS
ATTGAAGGTCTTGTTGTGTT+TGG 0.428205 5.4:-61474605 None:intergenic
GTAATGGCACAACGGAATCT+TGG 0.430159 5.4:-61474137 None:intergenic
CTTTGCAATCTGCTAGTTCA+AGG 0.430603 5.4:+61471096 MS.gene49190:CDS
TTTATTCATCCATCAATTGA+AGG 0.432413 5.4:-61474620 None:intergenic
TCGAACGCTCATTCGTGAAC+TGG 0.435177 5.4:+61474167 MS.gene49190:CDS
AGACTCAATAAAGAGAAGAT+TGG 0.442329 5.4:-61472183 None:intergenic
AATTACCTGAAAACCCATAC+TGG 0.452422 5.4:-61473898 None:intergenic
CAACCTCCCTGAGGTTCAAA+AGG 0.453733 5.4:+61473476 MS.gene49190:CDS
TTGTGGAACCAAGCTCCATA+TGG 0.454668 5.4:-61474219 None:intergenic
GGTCCTGGTTGTTCTTCCGT+TGG 0.459552 5.4:+61471962 MS.gene49190:intron
AACAGATGCAGTGCTCTAGA+AGG 0.459934 5.4:-61474554 None:intergenic
TACGAAAGAATTCAATGTCC+TGG 0.463993 5.4:+61472041 MS.gene49190:CDS
GTTAACTTTCGCCACCGTGC+GGG 0.467382 5.4:+61474502 MS.gene49190:CDS
ATTGAGTCTCCTGCTGGAGT+TGG 0.467432 5.4:+61472197 MS.gene49190:CDS
ATATGCTTCCAATTCTCAAA+CGG 0.470413 5.4:+61473857 MS.gene49190:CDS
CTGCACATGGACCATGAATA+CGG 0.472089 5.4:-61473525 None:intergenic
CAGGCTACTAAAATAAGTAT+AGG 0.472450 5.4:+61473414 MS.gene49190:intron
AATTCAATGTCCTGGAATAG+AGG 0.472547 5.4:+61472049 MS.gene49190:CDS
TTCAAGATTACAGTCCCATA+TGG 0.472759 5.4:+61474204 MS.gene49190:CDS
CTATGTGTAGGTTGGAGGTT+GGG 0.476731 5.4:+61474460 MS.gene49190:intron
CTGTTCAGTAACTTTGTGAA+TGG 0.483667 5.4:+61474572 MS.gene49190:CDS
TGGACCTGCATAGCTTTCTC+CGG 0.483807 5.4:-61472493 None:intergenic
AACTAGCAGATTGCAAAGAT+AGG 0.487129 5.4:-61471091 None:intergenic
GAATTACAATGGCACAGTAT+TGG 0.487226 5.4:+61471043 None:intergenic
GAAGAACGAAAGCATATCAT+TGG 0.488029 5.4:-61472418 None:intergenic
AGTGATGCAAATGAAGTTGT+CGG 0.492780 5.4:+61473213 MS.gene49190:CDS
TCCGGTAAGAAACAACGCTC+TGG 0.495399 5.4:-61472475 None:intergenic
TGTGACCAGAAATAATCATA+TGG 0.497544 5.4:-61473081 None:intergenic
TCAGTAACTTTGTGAATGGT+AGG 0.501716 5.4:+61474576 MS.gene49190:CDS
GGTTGTTCTTCCGTTGGAGG+AGG 0.506091 5.4:+61471968 MS.gene49190:CDS
CCATCACCAGTAGGATAAAA+CGG 0.509390 5.4:-61472010 None:intergenic
TGAAAACCCATACTGGAATA+CGG 0.509668 5.4:-61473891 None:intergenic
TCAAAACCGTATTCCAGTAT+GGG 0.511117 5.4:+61473885 MS.gene49190:CDS
GGTTACAAGTTCAATTTGAA+AGG 0.512302 5.4:+61472902 MS.gene49190:CDS
GTTGAATTCAGTCAATATGC+TGG 0.514110 5.4:+61471165 MS.gene49190:CDS
AGTTCTTGTTCAACTATGGA+TGG 0.514324 5.4:-61473279 None:intergenic
TCCATTAAGCCAAAGAGTGA+GGG 0.516289 5.4:-61471268 None:intergenic
CATGATGCTGATACATTATG+AGG 0.518908 5.4:-61473177 None:intergenic
TTACAGTCCCATATGGAGCT+TGG 0.523649 5.4:+61474211 MS.gene49190:CDS
CTTACCGGAGAAAGCTATGC+AGG 0.524600 5.4:+61472489 MS.gene49190:CDS
TGTGGAACCAAGCTCCATAT+GGG 0.530718 5.4:-61474218 None:intergenic
TAACAACCAGATCCTCAATA+GGG 0.533256 5.4:-61471122 None:intergenic
CATATCTATGTGTAGGTTGG+AGG 0.535597 5.4:+61474455 MS.gene49190:intron
TATCCTACTGGTGATGGACG+TGG 0.538754 5.4:+61472016 MS.gene49190:CDS
AGTCTCCTGCTGGAGTTGGT+TGG 0.541071 5.4:+61472201 MS.gene49190:CDS
TTAACTTTCGCCACCGTGCG+GGG 0.550282 5.4:+61474503 MS.gene49190:CDS
AGTTCAAGGGTACCCTATTG+AGG 0.551760 5.4:+61471110 MS.gene49190:CDS
TTATACTTACCCACTGCACA+TGG 0.557753 5.4:-61473538 None:intergenic
GCAAGTTGGTACGGTTCGCA+TGG 0.558199 5.4:-61473503 None:intergenic
TTTGCAATCTGCTAGTTCAA+GGG 0.559131 5.4:+61471097 MS.gene49190:CDS
TCTATGTGTAGGTTGGAGGT+TGG 0.559649 5.4:+61474459 MS.gene49190:intron
AAACGGACCTAATTCAGTAA+AGG 0.561047 5.4:-61471993 None:intergenic
AACTTCATTTGCATCACTGA+TGG 0.561383 5.4:-61473208 None:intergenic
TATGACCAACCAACTCCAGC+AGG 0.564584 5.4:-61472206 None:intergenic
TATTCATGGTCCATGTGCAG+TGG 0.566051 5.4:+61473528 MS.gene49190:CDS
CTCGATCCAGATTCAAAAGT+GGG 0.567933 5.4:-61473047 None:intergenic
CGTGCGGGGTGCATCTCACA+TGG 0.568257 5.4:+61474517 MS.gene49190:CDS
AGCTTGGTTCCACAAAGGCC+AGG 0.580658 5.4:+61474227 MS.gene49190:CDS
ATGAATACGGCAAGTTGGTA+CGG 0.581357 5.4:-61473512 None:intergenic
CTCCATTAAGCCAAAGAGTG+AGG 0.581909 5.4:-61471269 None:intergenic
CGATTACAACATCGGTGACG+AGG 0.590138 5.4:+61472244 MS.gene49190:CDS
AATTCAACTTTAGGTTGACC+AGG 0.595792 5.4:-61471150 None:intergenic
TCTCGATCCAGATTCAAAAG+TGG 0.599175 5.4:-61473048 None:intergenic
TATGATTATTTCTGGTCACA+TGG 0.601807 5.4:+61473084 MS.gene49190:CDS
CTCACTCTTTGGCTTAATGG+AGG 0.603231 5.4:+61471270 MS.gene49190:CDS
CCTCCAACGGAAGAACAACC+AGG 0.604280 5.4:-61471965 None:intergenic
GGACCATGAATACGGCAAGT+TGG 0.604501 5.4:-61473517 None:intergenic
AGTAAAGGCACCTCCTCCAA+CGG 0.608931 5.4:-61471978 None:intergenic
ATTCATGGTCCATGTGCAGT+GGG 0.615927 5.4:+61473529 MS.gene49190:CDS
TGTTAACTTTCGCCACCGTG+CGG 0.616872 5.4:+61474501 MS.gene49190:CDS
AACGCTCTGGATTTGTAGGT+AGG 0.617990 5.4:-61472462 None:intergenic
ACAACTTCCGATTACAACAT+CGG 0.619469 5.4:+61472236 MS.gene49190:CDS
TACATTATGAGGAGATGCGA+AGG 0.628696 5.4:-61473166 None:intergenic
AACATCGGTGACGAGGCCAC+AGG 0.633390 5.4:+61472251 MS.gene49190:CDS
CCATTAAGCCAAAGAGTGAG+GGG 0.635706 5.4:-61471267 None:intergenic
AGCGTTCGAGAACCTAGTAA+TGG 0.641706 5.4:-61474153 None:intergenic
AGAATAGCATTAGCTAACTG+AGG 0.658804 5.4:-61472857 None:intergenic
CATGTGAGATGCACCCCGCA+CGG 0.659786 5.4:-61474516 None:intergenic
GTTGATATTGATGTAAAACA+TGG 0.675625 5.4:+61471192 MS.gene49190:CDS
ACACCACGTCCATCACCAGT+AGG 0.679633 5.4:-61472019 None:intergenic
TATGGAGCTTGGTTCCACAA+AGG 0.680414 5.4:+61474222 MS.gene49190:CDS
GTGAGATGCACCCCGCACGG+TGG 0.715495 5.4:-61474513 None:intergenic
AGAACCTAGTAATGGCACAA+CGG 0.721630 5.4:-61474145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAATAATTAAAATT+GGG - chr5.4:61472538-61472557 None:intergenic 0.0%
!!! TTTTATTATTATTTTTTTTT+TGG + chr5.4:61471416-61471435 MS.gene49190:intron 0.0%
!! AATATAAGATATCATATGAA+TGG - chr5.4:61472943-61472962 None:intergenic 15.0%
!! AGATAATAAAAATAGAAAAG+AGG - chr5.4:61472757-61472776 None:intergenic 15.0%
!! ATACTTTATAATGTTTATTC+AGG + chr5.4:61472824-61472843 MS.gene49190:intron 15.0%
!! ATATAAGATATCATATGAAT+GGG - chr5.4:61472942-61472961 None:intergenic 15.0%
!! GATAAAGTATAGTATTTATA+GGG - chr5.4:61473975-61473994 None:intergenic 15.0%
!! TGATAAAGTATAGTATTTAT+AGG - chr5.4:61473976-61473995 None:intergenic 15.0%
!! TTCATATGATATCTTATATT+TGG + chr5.4:61472943-61472962 MS.gene49190:intron 15.0%
!!! ATAATCCTTTAATTTGTTTT+TGG + chr5.4:61472392-61472411 MS.gene49190:intron 15.0%
!! AACTGTTAAAAATAATTACC+TGG - chr5.4:61474248-61474267 None:intergenic 20.0%
!! AGTGTTTAATTAAGAAATCT+GGG + chr5.4:61472972-61472991 MS.gene49190:intron 20.0%
!! GTAAACACAATATTAAAATC+AGG - chr5.4:61472146-61472165 None:intergenic 20.0%
!! GTATAAATTAAATAGCCAAT+TGG - chr5.4:61473941-61473960 None:intergenic 20.0%
!! TAAAATTCAAATTGCATGTT+TGG - chr5.4:61471552-61471571 None:intergenic 20.0%
!! TAGTATAATAGAAATGAAAG+AGG - chr5.4:61473332-61473351 None:intergenic 20.0%
!!! AGTAACTTTGTTAATTTTTC+AGG + chr5.4:61471941-61471960 MS.gene49190:intron 20.0%
!!! ATTTTGATTATTATTGTTGC+TGG + chr5.4:61471348-61471367 MS.gene49190:intron 20.0%
!!! TTTGTTAGTTTTATAATGCA+TGG + chr5.4:61472367-61472386 MS.gene49190:intron 20.0%
!!! TTTTACATGTTGTTATCTTT+TGG + chr5.4:61471567-61471586 MS.gene49190:intron 20.0%
!!! TTTTTTTTTTTGTAAATCGG+CGG + chr5.4:61471851-61471870 MS.gene49190:intron 20.0%
! AAAATTGTTCATGAGAACAA+AGG - chr5.4:61472791-61472810 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG + chr5.4:61474294-61474313 MS.gene49190:intron 25.0%
! AGTATAGCATCTTTCATATA+AGG - chr5.4:61471799-61471818 None:intergenic 25.0%
! GAGTGTTTAATTAAGAAATC+TGG + chr5.4:61472971-61472990 MS.gene49190:intron 25.0%
! GTGTTAAATTATTATGACAG+TGG + chr5.4:61474111-61474130 MS.gene49190:intron 25.0%
! GTTGATATTGATGTAAAACA+TGG + chr5.4:61471192-61471211 MS.gene49190:CDS 25.0%
! TCGAAAAAAATCAACATGTA+AGG - chr5.4:61473668-61473687 None:intergenic 25.0%
! TGTCGCTAATAATAGTTTAT+GGG - chr5.4:61473581-61473600 None:intergenic 25.0%
! TTTCAAATTGAACTTGTAAC+CGG - chr5.4:61472903-61472922 None:intergenic 25.0%
! TTTGTGTATGTTTGAATTGA+CGG + chr5.4:61471440-61471459 MS.gene49190:intron 25.0%
!! ATTGGAAGCATATTAATGTT+AGG - chr5.4:61473850-61473869 None:intergenic 25.0%
!! TATTGACTGAATTCAACTTT+AGG - chr5.4:61471162-61471181 None:intergenic 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG - chr5.4:61474274-61474293 None:intergenic 25.0%
AAAATTGATACCTCTATTCC+AGG - chr5.4:61472062-61472081 None:intergenic 30.0%
AAAGTTTAAATCAGTACCTG+TGG - chr5.4:61472270-61472289 None:intergenic 30.0%
AGAACTGAGATTGAAAAAGA+TGG + chr5.4:61473296-61473315 MS.gene49190:CDS 30.0%
AGACTCAATAAAGAGAAGAT+TGG - chr5.4:61472186-61472205 None:intergenic 30.0%
ATATGCTTCCAATTCTCAAA+CGG + chr5.4:61473857-61473876 MS.gene49190:CDS 30.0%
CAAATTCAAATCCTGTTACA+AGG + chr5.4:61471883-61471902 MS.gene49190:intron 30.0%
CAGGCTACTAAAATAAGTAT+AGG + chr5.4:61473414-61473433 MS.gene49190:intron 30.0%
GCATCTTTCATATAAGGTAA+AGG - chr5.4:61471793-61471812 None:intergenic 30.0%
GGAATGATTTCAGATGAAAT+TGG + chr5.4:61473105-61473124 MS.gene49190:CDS 30.0%
GGTTACAAGTTCAATTTGAA+AGG + chr5.4:61472902-61472921 MS.gene49190:CDS 30.0%
GTTTCGAAATTCTCAAAAGT+TGG + chr5.4:61472559-61472578 MS.gene49190:intron 30.0%
TCATCACTGTTGTCAAATAA+AGG + chr5.4:61474362-61474381 MS.gene49190:intron 30.0%
TGCATAAAGAATACAATACG+AGG - chr5.4:61472644-61472663 None:intergenic 30.0%
TGTGACCAGAAATAATCATA+TGG - chr5.4:61473084-61473103 None:intergenic 30.0%
TTGATTGCATATCTATGTGT+AGG + chr5.4:61474448-61474467 MS.gene49190:intron 30.0%
TTTCAACAAAAGAGACCAAT+TGG + chr5.4:61473923-61473942 MS.gene49190:intron 30.0%
TTTGACAAGAACAGAATCTT+TGG + chr5.4:61471711-61471730 MS.gene49190:intron 30.0%
! AAAATCGCAGTCTTTTGTAA+TGG - chr5.4:61473136-61473155 None:intergenic 30.0%
! AAGACGATATCTCTAGTTTA+TGG - chr5.4:61473612-61473631 None:intergenic 30.0%
! AGACGATATCTCTAGTTTAT+GGG - chr5.4:61473611-61473630 None:intergenic 30.0%
! ATTAGCTTCTTAGTAATCAG+AGG - chr5.4:61474088-61474107 None:intergenic 30.0%
! GATCTGATTAATGTGTCTTT+TGG - chr5.4:61472516-61472535 None:intergenic 30.0%
! GTGTCGCTAATAATAGTTTA+TGG - chr5.4:61473582-61473601 None:intergenic 30.0%
!! GATTTTAATGCTCATTCAAC+CGG + chr5.4:61472881-61472900 MS.gene49190:CDS 30.0%
!! TATGATTATTTCTGGTCACA+TGG + chr5.4:61473084-61473103 MS.gene49190:CDS 30.0%
!!! GTAATCTAAGCTTTTTTCTC+TGG + chr5.4:61473007-61473026 MS.gene49190:intron 30.0%
!!! TTTGTTAATTTTTCAGGTCC+TGG + chr5.4:61471947-61471966 MS.gene49190:intron 30.0%
AAAATAAACCCCTCACTCTT+TGG + chr5.4:61471259-61471278 MS.gene49190:CDS 35.0%
AAACGGACCTAATTCAGTAA+AGG - chr5.4:61471996-61472015 None:intergenic 35.0%
AACTAGCAGATTGCAAAGAT+AGG - chr5.4:61471094-61471113 None:intergenic 35.0%
AACTTCATTTGCATCACTGA+TGG - chr5.4:61473211-61473230 None:intergenic 35.0%
AATATCCCAGTTAGCAATGA+TGG - chr5.4:61472684-61472703 None:intergenic 35.0%
AATTACCTGAAAACCCATAC+TGG - chr5.4:61473901-61473920 None:intergenic 35.0%
AATTCAATGTCCTGGAATAG+AGG + chr5.4:61472049-61472068 MS.gene49190:CDS 35.0%
ACAACAAGACCTTCAATTGA+TGG + chr5.4:61474611-61474630 MS.gene49190:CDS 35.0%
ACAACTCAACCTGAATTTGT+AGG - chr5.4:61471657-61471676 None:intergenic 35.0%
ACAACTTCCGATTACAACAT+CGG + chr5.4:61472236-61472255 MS.gene49190:CDS 35.0%
AGAATAGCATTAGCTAACTG+AGG - chr5.4:61472860-61472879 None:intergenic 35.0%
AGTGATGCAAATGAAGTTGT+CGG + chr5.4:61473213-61473232 MS.gene49190:CDS 35.0%
AGTTCTTGTTCAACTATGGA+TGG - chr5.4:61473282-61473301 None:intergenic 35.0%
CAACTCAACCTGAATTTGTA+GGG - chr5.4:61471656-61471675 None:intergenic 35.0%
CATGATGCTGATACATTATG+AGG - chr5.4:61473180-61473199 None:intergenic 35.0%
CTGTTCAGTAACTTTGTGAA+TGG + chr5.4:61474572-61474591 MS.gene49190:CDS 35.0%
GAGAGTGAGTAAAACTTGTA+AGG - chr5.4:61474028-61474047 None:intergenic 35.0%
GAGCACCATATGATTATTTC+TGG + chr5.4:61473076-61473095 MS.gene49190:CDS 35.0%
GGCTGCCAAAAACAAATTAA+AGG - chr5.4:61472400-61472419 None:intergenic 35.0%
GTTGAATTCAGTCAATATGC+TGG + chr5.4:61471165-61471184 MS.gene49190:CDS 35.0%
TAACAACCAGATCCTCAATA+GGG - chr5.4:61471125-61471144 None:intergenic 35.0%
TACGAAAGAATTCAATGTCC+TGG + chr5.4:61472041-61472060 MS.gene49190:CDS 35.0%
TCAAAACCGTATTCCAGTAT+GGG + chr5.4:61473885-61473904 MS.gene49190:CDS 35.0%
TCAGTAACTTTGTGAATGGT+AGG + chr5.4:61474576-61474595 MS.gene49190:CDS 35.0%
TCTCAGTTCTTGTTCAACTA+TGG - chr5.4:61473286-61473305 None:intergenic 35.0%
TGAAAACCCATACTGGAATA+CGG - chr5.4:61473894-61473913 None:intergenic 35.0%
TGAACAATCCGTTTGAGAAT+TGG - chr5.4:61473868-61473887 None:intergenic 35.0%
TTCAAAACCGTATTCCAGTA+TGG + chr5.4:61473884-61473903 MS.gene49190:CDS 35.0%
TTCAAGATTACAGTCCCATA+TGG + chr5.4:61474204-61474223 MS.gene49190:CDS 35.0%
TTGCATATCTATGTGTAGGT+TGG + chr5.4:61474452-61474471 MS.gene49190:intron 35.0%
TTTGCAATCTGCTAGTTCAA+GGG + chr5.4:61471097-61471116 MS.gene49190:CDS 35.0%
! AAATAATTACCTGGCCTTTG+TGG - chr5.4:61474239-61474258 None:intergenic 35.0%
! ACTCCAGTTTTCCAAATCAA+CGG + chr5.4:61474333-61474352 MS.gene49190:intron 35.0%
! GAAGAACGAAAGCATATCAT+TGG - chr5.4:61472421-61472440 None:intergenic 35.0%
! GCTTTTCTAACAGTTGACAA+TGG + chr5.4:61471757-61471776 MS.gene49190:intron 35.0%
! TACAAGTTTTACTCACTCTC+AGG + chr5.4:61474028-61474047 MS.gene49190:intron 35.0%
! TGTAACTTTTGTCCAAATCG+CGG + chr5.4:61471499-61471518 MS.gene49190:intron 35.0%
!! AATTCAACTTTAGGTTGACC+AGG - chr5.4:61471153-61471172 None:intergenic 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG - chr5.4:61474608-61474627 None:intergenic 35.0%
!! TTTCTCTGGCTTTGTTAGAT+TGG + chr5.4:61473021-61473040 MS.gene49190:intron 35.0%
ACTAAATCACACTACAACCG+TGG - chr5.4:61471480-61471499 None:intergenic 40.0%
AGAACCTAGTAATGGCACAA+CGG - chr5.4:61474148-61474167 None:intergenic 40.0%
CCATCACCAGTAGGATAAAA+CGG - chr5.4:61472013-61472032 None:intergenic 40.0%
CTAACAACCAGATCCTCAAT+AGG - chr5.4:61471126-61471145 None:intergenic 40.0%
CTATGCCATCATTGCTAACT+GGG + chr5.4:61472676-61472695 MS.gene49190:intron 40.0%
CTCGATCCAGATTCAAAAGT+GGG - chr5.4:61473050-61473069 None:intergenic 40.0%
CTTTGCAATCTGCTAGTTCA+AGG + chr5.4:61471096-61471115 MS.gene49190:CDS 40.0%
GAAACTTCTCGAACCATTTC+AGG - chr5.4:61472443-61472462 None:intergenic 40.0%
GGACTGTAATCTTGAACTTC+AGG - chr5.4:61474200-61474219 None:intergenic 40.0%
TACATTATGAGGAGATGCGA+AGG - chr5.4:61473169-61473188 None:intergenic 40.0%
TCCATTAAGCCAAAGAGTGA+GGG - chr5.4:61471271-61471290 None:intergenic 40.0%
TCTCGATCCAGATTCAAAAG+TGG - chr5.4:61473051-61473070 None:intergenic 40.0%
TGCTTTCGTTCTTCCTGAAA+TGG + chr5.4:61472427-61472446 MS.gene49190:CDS 40.0%
TGTGTTGTCCCTACAAATTC+AGG + chr5.4:61471645-61471664 MS.gene49190:intron 40.0%
TTATACTTACCCACTGCACA+TGG - chr5.4:61473541-61473560 None:intergenic 40.0%
TTCGTCTATTTCGTTACTGC+AGG + chr5.4:61473395-61473414 MS.gene49190:intron 40.0%
! AAACAACGCTCTGGATTTGT+AGG - chr5.4:61472469-61472488 None:intergenic 40.0%
! ATTTTACTTCAACCTCCCTG+AGG + chr5.4:61473467-61473486 MS.gene49190:CDS 40.0%
! CAGATTATGAGCCTTGTAAC+AGG - chr5.4:61471897-61471916 None:intergenic 40.0%
! CATATCTATGTGTAGGTTGG+AGG + chr5.4:61474455-61474474 MS.gene49190:intron 40.0%
! GATGAAAACCACCGTTGATT+TGG - chr5.4:61474347-61474366 None:intergenic 40.0%
! GTATTCCAGTATGGGTTTTC+AGG + chr5.4:61473893-61473912 MS.gene49190:CDS 40.0%
! TTAGGTCCGTTTTATCCTAC+TGG + chr5.4:61472004-61472023 MS.gene49190:CDS 40.0%
!! ATGAATACGGCAAGTTGGTA+CGG - chr5.4:61473515-61473534 None:intergenic 40.0%
!!! AGAGTGAGGGGTTTATTTTG+AGG - chr5.4:61471258-61471277 None:intergenic 40.0%
AGCGTTCGAGAACCTAGTAA+TGG - chr5.4:61474156-61474175 None:intergenic 45.0%
ATAGTGACGCTATTGCGTAG+TGG + chr5.4:61474387-61474406 MS.gene49190:intron 45.0%
ATTCATGGTCCATGTGCAGT+GGG + chr5.4:61473529-61473548 MS.gene49190:CDS 45.0%
CCAGTTAGCAATGATGGCAT+AGG - chr5.4:61472678-61472697 None:intergenic 45.0%
CCATTAAGCCAAAGAGTGAG+GGG - chr5.4:61471270-61471289 None:intergenic 45.0%
CCTATGCCATCATTGCTAAC+TGG + chr5.4:61472675-61472694 MS.gene49190:intron 45.0%
CGGTGAGTTTACCAAAACCA+CGG + chr5.4:61471460-61471479 MS.gene49190:intron 45.0%
CTCCATTAAGCCAAAGAGTG+AGG - chr5.4:61471272-61471291 None:intergenic 45.0%
CTCGTCACCGATGTTGTAAT+CGG - chr5.4:61472246-61472265 None:intergenic 45.0%
CTCTTTATTGAGTCTCCTGC+TGG + chr5.4:61472191-61472210 MS.gene49190:CDS 45.0%
CTGCACATGGACCATGAATA+CGG - chr5.4:61473528-61473547 None:intergenic 45.0%
GTAATGGCACAACGGAATCT+TGG - chr5.4:61474140-61474159 None:intergenic 45.0%
GTACCAACTTGCCGTATTCA+TGG + chr5.4:61473514-61473533 MS.gene49190:CDS 45.0%
TATGGAGCTTGGTTCCACAA+AGG + chr5.4:61474222-61474241 MS.gene49190:CDS 45.0%
TATTCATGGTCCATGTGCAG+TGG + chr5.4:61473528-61473547 MS.gene49190:CDS 45.0%
TGTGGAACCAAGCTCCATAT+GGG - chr5.4:61474221-61474240 None:intergenic 45.0%
TTACAGTCCCATATGGAGCT+TGG + chr5.4:61474211-61474230 MS.gene49190:CDS 45.0%
TTGTGGAACCAAGCTCCATA+TGG - chr5.4:61474222-61474241 None:intergenic 45.0%
! AACAGATGCAGTGCTCTAGA+AGG - chr5.4:61474557-61474576 None:intergenic 45.0%
! AACGCTCTGGATTTGTAGGT+AGG - chr5.4:61472465-61472484 None:intergenic 45.0%
! AACTTTTGTCCAAATCGCGG+TGG + chr5.4:61471502-61471521 MS.gene49190:intron 45.0%
! CCAGTTTTCCAAATCAACGG+TGG + chr5.4:61474336-61474355 MS.gene49190:intron 45.0%
! CCGTTTTATCCTACTGGTGA+TGG + chr5.4:61472010-61472029 MS.gene49190:CDS 45.0%
! CGCAGTCTTTTGTAATGGCA+AGG - chr5.4:61473131-61473150 None:intergenic 45.0%
! CTATGTGTAGGTTGGAGGTT+GGG + chr5.4:61474460-61474479 MS.gene49190:intron 45.0%
! CTCACTCTTTGGCTTAATGG+AGG + chr5.4:61471270-61471289 MS.gene49190:CDS 45.0%
! GATTCCGTTGTGCCATTACT+AGG + chr5.4:61474141-61474160 MS.gene49190:CDS 45.0%
! TCACACTACAACCGTGGTTT+TGG - chr5.4:61471474-61471493 None:intergenic 45.0%
! TCTATGTGTAGGTTGGAGGT+TGG + chr5.4:61474459-61474478 MS.gene49190:intron 45.0%
!! AGTTCAAGGGTACCCTATTG+AGG + chr5.4:61471110-61471129 MS.gene49190:CDS 45.0%
!! CCACCGTTGATTTGGAAAAC+TGG - chr5.4:61474339-61474358 None:intergenic 45.0%
!! GATCTGGTTGTTAGTCTTCC+TGG + chr5.4:61471132-61471151 MS.gene49190:CDS 45.0%
!! GCTCTAGAAGGTTGTGCATA+TGG - chr5.4:61474545-61474564 None:intergenic 45.0%
!! GGAGGTGCCTTTACTGAATT+AGG + chr5.4:61471986-61472005 MS.gene49190:CDS 45.0%
!! TCCAGAGCGTTGTTTCTTAC+CGG + chr5.4:61472474-61472493 MS.gene49190:CDS 45.0%
!! TGGCAACCCACTTTTGAATC+TGG + chr5.4:61473041-61473060 MS.gene49190:CDS 45.0%
!! GATAAATTAATAATTAAAAT+TGG - chr5.4:61472539-61472558 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTGTAAAT+CGG + chr5.4:61471848-61471867 MS.gene49190:intron 5.0%
ATTGAGTCTCCTGCTGGAGT+TGG + chr5.4:61472197-61472216 MS.gene49190:CDS 50.0%
CAACCTCCCTGAGGTTCAAA+AGG + chr5.4:61473476-61473495 MS.gene49190:CDS 50.0%
CGATTACAACATCGGTGACG+AGG + chr5.4:61472244-61472263 MS.gene49190:CDS 50.0%
CTTACCGGAGAAAGCTATGC+AGG + chr5.4:61472489-61472508 MS.gene49190:CDS 50.0%
GGACCATGAATACGGCAAGT+TGG - chr5.4:61473520-61473539 None:intergenic 50.0%
TATGACCAACCAACTCCAGC+AGG - chr5.4:61472209-61472228 None:intergenic 50.0%
TCCGGTAAGAAACAACGCTC+TGG - chr5.4:61472478-61472497 None:intergenic 50.0%
TCGAACGCTCATTCGTGAAC+TGG + chr5.4:61474167-61474186 MS.gene49190:CDS 50.0%
TGGACCTGCATAGCTTTCTC+CGG - chr5.4:61472496-61472515 None:intergenic 50.0%
TGTTAACTTTCGCCACCGTG+CGG + chr5.4:61474501-61474520 MS.gene49190:CDS 50.0%
! AGGGTACCCTATTGAGGATC+TGG + chr5.4:61471116-61471135 MS.gene49190:CDS 50.0%
! CCCCTCACTCTTTGGCTTAA+TGG + chr5.4:61471267-61471286 MS.gene49190:CDS 50.0%
! GGAGAGCCTTTTGAACCTCA+GGG - chr5.4:61473485-61473504 None:intergenic 50.0%
! TGGAGAGCCTTTTGAACCTC+AGG - chr5.4:61473486-61473505 None:intergenic 50.0%
!! AGTAAAGGCACCTCCTCCAA+CGG - chr5.4:61471981-61472000 None:intergenic 50.0%
!! TATCCTACTGGTGATGGACG+TGG + chr5.4:61472016-61472035 MS.gene49190:CDS 50.0%
ACACCACGTCCATCACCAGT+AGG - chr5.4:61472022-61472041 None:intergenic 55.0%
AGCTTGGTTCCACAAAGGCC+AGG + chr5.4:61474227-61474246 MS.gene49190:CDS 55.0%
CCTCCAACGGAAGAACAACC+AGG - chr5.4:61471968-61471987 None:intergenic 55.0%
CCTGGTTGTTCTTCCGTTGG+AGG + chr5.4:61471965-61471984 MS.gene49190:CDS 55.0%
GGAGGTTGGGCAACTGAGTA+TGG + chr5.4:61474473-61474492 MS.gene49190:CDS 55.0%
GGTCCTGGTTGTTCTTCCGT+TGG + chr5.4:61471962-61471981 MS.gene49190:intron 55.0%
GGTTGTTCTTCCGTTGGAGG+AGG + chr5.4:61471968-61471987 MS.gene49190:CDS 55.0%
GTTAACTTTCGCCACCGTGC+GGG + chr5.4:61474502-61474521 MS.gene49190:CDS 55.0%
TTAACTTTCGCCACCGTGCG+GGG + chr5.4:61474503-61474522 MS.gene49190:CDS 55.0%
! AGTCTCCTGCTGGAGTTGGT+TGG + chr5.4:61472201-61472220 MS.gene49190:CDS 55.0%
! GAGCCTTTTGAACCTCAGGG+AGG - chr5.4:61473482-61473501 None:intergenic 55.0%
!! GCAAGTTGGTACGGTTCGCA+TGG - chr5.4:61473506-61473525 None:intergenic 55.0%
AACATCGGTGACGAGGCCAC+AGG + chr5.4:61472251-61472270 MS.gene49190:CDS 60.0%
CACGCTGAACCACCGCGATT+TGG - chr5.4:61471514-61471533 None:intergenic 60.0%
CATGTGAGATGCACCCCGCA+CGG - chr5.4:61474519-61474538 None:intergenic 60.0%
CGTGCGGGGTGCATCTCACA+TGG + chr5.4:61474517-61474536 MS.gene49190:CDS 65.0%
GTGAGATGCACCCCGCACGG+TGG - chr5.4:61474516-61474535 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 61471051 61474637 61471051 ID=MS.gene49190
chr5.4 mRNA 61471051 61474637 61471051 ID=MS.gene49190.t1;Parent=MS.gene49190
chr5.4 exon 61471051 61471291 61471051 ID=MS.gene49190.t1.exon1;Parent=MS.gene49190.t1
chr5.4 CDS 61471051 61471291 61471051 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61471963 61472070 61471963 ID=MS.gene49190.t1.exon2;Parent=MS.gene49190.t1
chr5.4 CDS 61471963 61472070 61471963 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61472180 61472272 61472180 ID=MS.gene49190.t1.exon3;Parent=MS.gene49190.t1
chr5.4 CDS 61472180 61472272 61472180 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61472418 61472510 61472418 ID=MS.gene49190.t1.exon4;Parent=MS.gene49190.t1
chr5.4 CDS 61472418 61472510 61472418 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61472846 61472931 61472846 ID=MS.gene49190.t1.exon5;Parent=MS.gene49190.t1
chr5.4 CDS 61472846 61472931 61472846 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61473039 61473317 61473039 ID=MS.gene49190.t1.exon6;Parent=MS.gene49190.t1
chr5.4 CDS 61473039 61473317 61473039 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61473417 61473550 61473417 ID=MS.gene49190.t1.exon7;Parent=MS.gene49190.t1
chr5.4 CDS 61473417 61473550 61473417 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61473822 61473914 61473822 ID=MS.gene49190.t1.exon8;Parent=MS.gene49190.t1
chr5.4 CDS 61473822 61473914 61473822 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61474131 61474248 61474131 ID=MS.gene49190.t1.exon9;Parent=MS.gene49190.t1
chr5.4 CDS 61474131 61474248 61474131 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
chr5.4 exon 61474470 61474637 61474470 ID=MS.gene49190.t1.exon10;Parent=MS.gene49190.t1
chr5.4 CDS 61474470 61474637 61474470 ID=cds.MS.gene49190.t1;Parent=MS.gene49190.t1
Gene Sequence

>MS.gene49190

ATGGCACAGTATTGGTTTCTTAGTGTATTGATTGCTGTGTCCTATCTTTGCAATCTGCTAGTTCAAGGGTACCCTATTGAGGATCTGGTTGTTAGTCTTCCTGGTCAACCTAAAGTTGAATTCAGTCAATATGCTGGTTATGTTGATATTGATGTAAAACATGGTAGAAGTTTGTTTTATTATTTTGTTGAAGCTGATCATAAACCTCAAAATAAACCCCTCACTCTTTGGCTTAATGGAGGTAAATAATAATTCACTAATATATTATATATCATCACTTTTAAATCATCACTTTTAATTTTGATTATTATTGTTGCTGGCTTAATTTTCTTGTTTTATTATTTTCAGTATATTTTTTGTTATCTTTTTATTATTATTTTTTTTTTGGTTTTGTGTATGTTTGAATTGACGGTGAGTTTACCAAAACCACGGTTGTAGTGTGATTTAGTGTAACTTTTGTCCAAATCGCGGTGGTTCAGCGTGATTTTGACAATTAGACCAAACATGCAATTTGAATTTTACATGTTGTTATCTTTTGGTGAAAAGCATTGTACTTGTGTTGTTTTGTGTGTGTTGTTTTGTGTTATGTTATGTTGTGTTGTCCCTACAAATTCAGGTTGAGTTGTTGTTCTTGTCTTGATAGTACAGAATCATGTTTTCTTTGACAAGAACAGAATCTTTGGATCAAAAATGTTGAATGATTCTTGCTTTTCTAACAGTTGACAATGGACAGAAAGTACCTTTACCTTATATGAAAGATGCTATACTTTAATTTTATTTTTTTCATAGAGTTTTTTTTTTTTTTTTTTTTGTAAATCGGCGGCATCAGATTCAAATTCAAATCCTGTTACAAGGCTCATAATCTGTATATTAATTGAGAGAGATTAAGTAGTAACTTTGTTAATTTTTCAGGTCCTGGTTGTTCTTCCGTTGGAGGAGGTGCCTTTACTGAATTAGGTCCGTTTTATCCTACTGGTGATGGACGTGGTGTACGAAAGAATTCAATGTCCTGGAATAGAGGTATCAATTTTGTTTATTATTGTTTGAGTTTGTGTTTTGATTGATATTAGAGAATAATTTTATATATAGATTCCTGATTTTAATATTGTGTTTACTTTTTTTATCATAGCTTCCAATCTTCTCTTTATTGAGTCTCCTGCTGGAGTTGGTTGGTCATACTCAAATACAACTTCCGATTACAACATCGGTGACGAGGCCACAGGTACTGATTTAAACTTTTCTGTGAACAAATTTATATGTAGTTATTATTATCTATGCAAAAGAAAACATTATTTTTAGATGTGTTATGTATGAAATTTGTTAGTTTTATAATGCATGGATATAATCCTTTAATTTGTTTTTGGCAGCCAATGATATGCTTTCGTTCTTCCTGAAATGGTTCGAGAAGTTTCCTACCTACAAATCCAGAGCGTTGTTTCTTACCGGAGAAAGCTATGCAGGTCCAAAAGACACATTAATCAGATCCCAATTTTAATTATTAATTTATCGTTTCGAAATTCTCAAAAGTTGGTCTCGTGTTACAAAATATTCTGTTTTGCTGACATAATTCAGTTATACTGCATAATTAAACCTCGTATTGTATTCTTTATGCATATGCACATAACCTATGCCATCATTGCTAACTGGGATATTGCTATACAATTATTATCTCATTTTATTTATTGTTTATTGAAATATTTGTTCCTCTTTTCTATTTTTATTATCTGTTTTCTTTTACCTTTGTTCTCATGAACAATTTTTGTTTCTTTACTAATACTTTATAATGTTTATTCAGGACATTACATTCCTCAGTTAGCTAATGCTATTCTTGATTTTAATGCTCATTCAACCGGTTACAAGTTCAATTTGAAAGGAGTAGCTGTAAGATCCCATTCATATGATATCTTATATTTGGCTATTGAGTGTTTAATTAAGAAATCTGGGAATTTAATTAAAGTAATCTAAGCTTTTTTCTCTGGCTTTGTTAGATTGGCAACCCACTTTTGAATCTGGATCGAGATGCACGAGCACCATATGATTATTTCTGGTCACATGGAATGATTTCAGATGAAATTGGCCTTGCCATTACAAAAGACTGCGATTTTGATGATTATACCTTCGCATCTCCTCATAATGTATCAGCATCATGCAACACTGCCATCAGTGATGCAAATGAAGTTGTCGGCAATTATATAAACAACTATGATGTGATTCTTGATGTTTGTTATCCATCCATAGTTGAACAAGAACTGAGATTGAAAAAGATGGTACTTTTTTGCCTCTTTCATTTCTATTATACTACTAATGATATTTTTAACTTATGATGAAATTATAAACTAATTAATTCGTCTATTTCGTTACTGCAGGCTACTAAAATAAGTATAGGTGTAGATGTCTGTATGAGTTATGAAAGAAAATTTTACTTCAACCTCCCTGAGGTTCAAAAGGCTCTCCATGCGAACCGTACCAACTTGCCGTATTCATGGTCCATGTGCAGTGGGTAAGTATAAATTAGAGACAGTCAAGTCCCATAAACTATTATTAGCGACACTCAAGTCCCATAAACTAGAGATATCGTCTTTTAATATAGCTTCTAAAATATAAACAATCATTGCCTTACATGTTGATTTTTTTCGATTGAGTTTTGCTATTTGAGATTTTTAAACTTACTTGAAATCTTGACTGCTATAATCATAATAGATATGTCTTTTTTTTTTTTTTCATTTTCATTAGTGATCTGATGTCTGATCTGCAGTTTTTCTTCATTGCAGTGTTTTAAATTACAGTGATACAGATCCTAACATTAATATGCTTCCAATTCTCAAACGGATTGTTCAAAACCGTATTCCAGTATGGGTTTTCAGGTAATTAATTTCAACAAAAGAGACCAATTGGCTATTTAATTTATACATTTTGATAGTCCCTATAAATACTATACTTTATCATTCATTTTACATCTTTTATCAGAACGACTCCTTACAAGTTTTACTCACTCTCAGGCTTTCAAATCTCTATTGATCATTTCTCTTGAAATCCTCTGATTACTAAGAAGCTAATCATGTGTTAAATTATTATGACAGTGGAGACCAAGATTCCGTTGTGCCATTACTAGGTTCTCGAACGCTCATTCGTGAACTGGCTGATGACCTGAAGTTCAAGATTACAGTCCCATATGGAGCTTGGTTCCACAAAGGCCAGGTAATTATTTTTAACAGTTTAACCATTTTCTGCATTTCGAAAAATAAGTTCAGTATTCTATAGTATGGTTATGCAATTTTTTTCACTCCAGTTTTCCAAATCAACGGTGGTTTTCATCACTGTTGTCAAATAAAGGCTATAGTGACGCTATTGCGTAGTGGAATTTAAACAAATCATTATTGTTATGAGTAAGAGTTGATTGATTGCATATCTATGTGTAGGTTGGAGGTTGGGCAACTGAGTATGGAAATTTGTTAACTTTCGCCACCGTGCGGGGTGCATCTCACATGGTACCATATGCACAACCTTCTAGAGCACTGCATCTGTTCAGTAACTTTGTGAATGGTAGGAGATTGCCAAACACAACAAGACCTTCAATTGATGGATGA
Protein sequence

>MS.gene49190.t1

MAQYWFLSVLIAVSYLCNLLVQGYPIEDLVVSLPGQPKVEFSQYAGYVDIDVKHGRSLFYYFVEADHKPQNKPLTLWLNGGPGCSSVGGGAFTELGPFYPTGDGRGVRKNSMSWNRASNLLFIESPAGVGWSYSNTTSDYNIGDEATANDMLSFFLKWFEKFPTYKSRALFLTGESYAGHYIPQLANAILDFNAHSTGYKFNLKGVAIGNPLLNLDRDARAPYDYFWSHGMISDEIGLAITKDCDFDDYTFASPHNVSASCNTAISDANEVVGNYINNYDVILDVCYPSIVEQELRLKKMATKISIGVDVCMSYERKFYFNLPEVQKALHANRTNLPYSWSMCSGVLNYSDTDPNINMLPILKRIVQNRIPVWVFSGDQDSVVPLLGSRTLIRELADDLKFKITVPYGAWFHKGQVGGWATEYGNLLTFATVRGASHMVPYAQPSRALHLFSNFVNGRRLPNTTRPSIDG