AlfalfaGEDB | MS.gene49418

KEGG: K22066
GO: -
PFAM: PF01722.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49418.t1	AES76469.1	95.2	105	5	0	1	105	1	105	4.60E-45	190.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49418.t1	Q682I1	61.8	89	34	0	11	99	67	155	1.7e-24	113.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49418.t1	G7KL80	95.2	105	5	0	1	105	1	105	3.3e-45	190.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051832	MS.gene49418	-0.804738	1.87E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49418.t1	MTR_6g079650	95.238	105	5	0	1	105	1	105	1.29e-70	206
MS.gene49418.t1	MTR_1g047990	58.696	92	37	1	9	100	260	350	1.56e-32	116

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49418.t1	AT1G55805	61.798	89	34	0	11	99	67	155	3.37e-36	120
MS.gene49418.t1	AT4G26500	59.184	98	38	1	5	100	274	371	1.41e-34	122

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGATGATGAATTGAAATC+TGG	0.289460	6.2:-77026756	MS.gene49418:CDS
CACTTCAGGGGTAATTTCCT+TGG	0.302109	6.2:+77026701	None:intergenic
ACACGTCGTCTAGCTCTATA+AGG	0.321269	6.2:+77026910	None:intergenic
GCACCGTATCATTTCACTTC+AGG	0.325677	6.2:+77026687	None:intergenic
TTTCAATTTGAAAATCGTTT+CGG	0.390771	6.2:-77026832	MS.gene49418:CDS
CTCTATAAGGGTGGCGGAGA+GGG	0.419218	6.2:+77026923	None:intergenic
ACTTCAGGGGTAATTTCCTT+GGG	0.433764	6.2:+77026702	None:intergenic
CACCGTATCATTTCACTTCA+GGG	0.453061	6.2:+77026688	None:intergenic
GGGTGGCGGAGAGGGATGAT+TGG	0.453980	6.2:+77026931	None:intergenic
TCTTGTTAAACGACATCGTA+TGG	0.487307	6.2:-77026790	MS.gene49418:CDS
TTGATGATGAATTGAAATCT+GGG	0.490766	6.2:-77026755	MS.gene49418:CDS
ATCGTTTCGGAGAAATTCGA+AGG	0.523092	6.2:-77026819	MS.gene49418:CDS
CATTGTTGCGAAAACGCCCA+AGG	0.564394	6.2:-77026718	MS.gene49418:CDS
CACGTCGTCTAGCTCTATAA+GGG	0.584411	6.2:+77026911	None:intergenic
GTCTAGCTCTATAAGGGTGG+CGG	0.602686	6.2:+77026917	None:intergenic
GCTCTATAAGGGTGGCGGAG+AGG	0.611742	6.2:+77026922	None:intergenic
ACCGTATCATTTCACTTCAG+GGG	0.622027	6.2:+77026689	None:intergenic
GGCGCGTGAGAGCAGTGTGG+TGG	0.631194	6.2:+77026974	None:intergenic
GTCGTCTAGCTCTATAAGGG+TGG	0.691485	6.2:+77026914	None:intergenic
GACGTGTCGTATCAACACGC+AGG	0.719674	6.2:-77026894	MS.gene49418:CDS
GGTGGCGCGTGAGAGCAGTG+TGG	0.785749	6.2:+77026971	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTCAATTTGAAAATCGTTT+CGG	-	chr6.2:77026853-77026872	MS.gene49418:CDS	20.0%
!!!	AAACGATTTTCAAATTGAAA+TGG	+	chr6.2:77026853-77026872	None:intergenic	20.0%
!!!	AACGATTTTCAAATTGAAAT+GGG	+	chr6.2:77026852-77026871	None:intergenic	20.0%
!!!	TTGATGATGAATTGAAATCT+GGG	-	chr6.2:77026930-77026949	MS.gene49418:CDS	25.0%
!!	CTTGATGATGAATTGAAATC+TGG	-	chr6.2:77026929-77026948	MS.gene49418:CDS	30.0%
	TCTTGTTAAACGACATCGTA+TGG	-	chr6.2:77026895-77026914	MS.gene49418:CDS	35.0%
!!!	TTGGAGTTTTGTTTGGATTC+TGG	+	chr6.2:77026738-77026757	None:intergenic	35.0%
	ACTTCAGGGGTAATTTCCTT+GGG	+	chr6.2:77026986-77027005	None:intergenic	40.0%
	ATCGTTTCGGAGAAATTCGA+AGG	-	chr6.2:77026866-77026885	MS.gene49418:CDS	40.0%
!!!	GAGTTTTGTTTGGATTCTGG+TGG	+	chr6.2:77026735-77026754	None:intergenic	40.0%
!!!	GGGATGATTGGAGTTTTGTT+TGG	+	chr6.2:77026745-77026764	None:intergenic	40.0%
	CACTTCAGGGGTAATTTCCT+TGG	+	chr6.2:77026987-77027006	None:intergenic	45.0%
!!	ACACGTCGTCTAGCTCTATA+AGG	+	chr6.2:77026778-77026797	None:intergenic	45.0%
!!	CACGTCGTCTAGCTCTATAA+GGG	+	chr6.2:77026777-77026796	None:intergenic	45.0%
	CATTGTTGCGAAAACGCCCA+AGG	-	chr6.2:77026967-77026986	MS.gene49418:CDS	50.0%
!	CTTGGGCGTTTTCGCAACAA+TGG	+	chr6.2:77026969-77026988	None:intergenic	50.0%
!!	GTCGTCTAGCTCTATAAGGG+TGG	+	chr6.2:77026774-77026793	None:intergenic	50.0%
!!	GTCTAGCTCTATAAGGGTGG+CGG	+	chr6.2:77026771-77026790	None:intergenic	50.0%
	GACGTGTCGTATCAACACGC+AGG	-	chr6.2:77026791-77026810	MS.gene49418:CDS	55.0%
!!	CTCTATAAGGGTGGCGGAGA+GGG	+	chr6.2:77026765-77026784	None:intergenic	55.0%
!!	GCTCTATAAGGGTGGCGGAG+AGG	+	chr6.2:77026766-77026785	None:intergenic	60.0%
!!	GGGTGGCGGAGAGGGATGAT+TGG	+	chr6.2:77026757-77026776	None:intergenic	65.0%
	GGCGCGTGAGAGCAGTGTGG+TGG	+	chr6.2:77026714-77026733	None:intergenic	70.0%
	GGTGGCGCGTGAGAGCAGTG+TGG	+	chr6.2:77026717-77026736	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	77026695	77027012	77026695	ID=MS.gene49418
chr6.2	mRNA	77026695	77027012	77026695	ID=MS.gene49418.t1;Parent=MS.gene49418
chr6.2	exon	77026695	77027012	77026695	ID=MS.gene49418.t1.exon1;Parent=MS.gene49418.t1
chr6.2	CDS	77026695	77027012	77026695	ID=cds.MS.gene49418.t1;Parent=MS.gene49418.t1

Gene Sequence	>MS.gene49418 ATGAGTATTCGCAGCACCACCACACTGCTCTCACGCGCCACCAGAATCCAAACAAAACTCCAATCATCCCTCTCCGCCACCCTTATAGAGCTAGACGACGTGTCGTATCAACACGCAGGTCACGCAGCAATGAAAGAATCATCTGAGCAAGAAACCCATTTCAATTTGAAAATCGTTTCGGAGAAATTCGAAGGATTGAATCTTGTTAAACGACATCGTATGGTTTATGAAGTTCTTGATGATGAATTGAAATCTGGGCTTCATGCTCTTTCCATTGTTGCGAAAACGCCCAAGGAAATTACCCCTGAAGTGAAATGA
Protein sequence	>MS.gene49418.t1 MSIRSTTTLLSRATRIQTKLQSSLSATLIELDDVSYQHAGHAAMKESSEQETHFNLKIVSEKFEGLNLVKRHRMVYEVLDDELKSGLHALSIVAKTPKEITPEVK

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M. sativa cultivar XinJiangDaYe / MS.gene49418