Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49596.t1 | XP_003613572.2 | 91 | 523 | 47 | 0 | 1 | 523 | 1 | 523 | 3.10E-243 | 850.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49596.t1 | Q9LFY0 | 35.8 | 530 | 300 | 13 | 9 | 521 | 25 | 531 | 1.1e-66 | 255.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49596.t1 | G7K9Q9 | 91.0 | 523 | 47 | 0 | 1 | 523 | 1 | 523 | 2.3e-243 | 850.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050623 | MS.gene49596 | -0.80174 | 7.81E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49596.t1 | MTR_5g038210 | 94.264 | 523 | 30 | 0 | 1 | 523 | 1 | 523 | 0.0 | 968 |
| MS.gene49596.t1 | MTR_3g065930 | 31.401 | 207 | 127 | 6 | 327 | 521 | 38 | 241 | 1.03e-15 | 77.4 |
| MS.gene49596.t1 | MTR_3g065930 | 29.434 | 265 | 153 | 7 | 289 | 521 | 38 | 300 | 1.82e-15 | 77.8 |
| MS.gene49596.t1 | MTR_3g065930 | 31.401 | 207 | 127 | 6 | 327 | 521 | 96 | 299 | 2.66e-15 | 77.0 |
| MS.gene49596.t1 | MTR_1g063080 | 32.414 | 145 | 86 | 1 | 389 | 521 | 476 | 620 | 4.11e-14 | 75.5 |
| MS.gene49596.t1 | MTR_7g113330 | 38.372 | 86 | 50 | 2 | 441 | 523 | 23 | 108 | 4.09e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49596.t1 | AT1G27070 | 40.977 | 532 | 270 | 13 | 9 | 521 | 25 | 531 | 1.15e-107 | 332 |
| MS.gene49596.t1 | AT5G03420 | 31.655 | 139 | 89 | 1 | 389 | 521 | 457 | 595 | 2.17e-14 | 76.3 |
| MS.gene49596.t1 | AT5G39790 | 38.182 | 110 | 59 | 3 | 416 | 521 | 168 | 272 | 7.39e-14 | 72.4 |
| MS.gene49596.t1 | AT5G39790 | 38.182 | 110 | 59 | 3 | 416 | 521 | 175 | 279 | 1.03e-13 | 72.0 |
| MS.gene49596.t1 | AT1G09020 | 40.476 | 84 | 48 | 1 | 440 | 521 | 18 | 101 | 1.25e-11 | 67.4 |
Find 110 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTGTAGGAAACCTTTC+TGG | 0.142621 | 5.1:-28823531 | None:intergenic |
| AATCCTGTTTCTCTGTTTAT+AGG | 0.161117 | 5.1:-28827802 | None:intergenic |
| AGCGAGTTTAGTTTGGTTCT+TGG | 0.262569 | 5.1:+28823357 | MS.gene49596:CDS |
| GGGAATGTAGCTAAGGCTTC+TGG | 0.265747 | 5.1:+28823672 | MS.gene49596:CDS |
| TTCTGATGCTTGGGAATTCC+AGG | 0.281647 | 5.1:+28829504 | MS.gene49596:CDS |
| CTAATCAACTCTTCCTTTGT+AGG | 0.289511 | 5.1:-28823543 | None:intergenic |
| GAAGGTGGGTGGTTATCTTA+TGG | 0.292331 | 5.1:+28823603 | MS.gene49596:CDS |
| AAAATCCCGTATTCAGCTTC+TGG | 0.294524 | 5.1:+28827867 | MS.gene49596:CDS |
| GGAGGTGCGAGCGATGTTTC+AGG | 0.297423 | 5.1:+28827739 | MS.gene49596:CDS |
| AATTCATGATTTCAGTTTCC+TGG | 0.300961 | 5.1:-28829522 | None:intergenic |
| TTTACTTACTCCATCTTTGA+TGG | 0.309971 | 5.1:-28824867 | None:intergenic |
| TAGAGGTGGGAGAGTGGATT+TGG | 0.344859 | 5.1:+28823563 | MS.gene49596:CDS |
| GTTTGTATTGGTCTTGTTCT+CGG | 0.350446 | 5.1:+28823424 | MS.gene49596:CDS |
| AAAGAGAGAAAGGGTCAAAT+GGG | 0.352398 | 5.1:-28823277 | None:intergenic |
| TGAAGCCGACTTGTGTAGTT+TGG | 0.362406 | 5.1:+28829768 | MS.gene49596:CDS |
| CGTGTTGATCAAGTTTGATC+TGG | 0.363057 | 5.1:-28827499 | None:intergenic |
| GGCACGTAACGAACGTAGTC+TGG | 0.370762 | 5.1:-28829551 | None:intergenic |
| AGAAATAGTTCTTTCGGACT+TGG | 0.376927 | 5.1:+28824776 | MS.gene49596:CDS |
| GCGAGTTTAGTTTGGTTCTT+GGG | 0.377017 | 5.1:+28823358 | MS.gene49596:CDS |
| GTTGGAGTTCATGCAGAATT+CGG | 0.384946 | 5.1:+28823503 | MS.gene49596:CDS |
| GTCTTTGGAGGAGGAGTTGT+TGG | 0.386494 | 5.1:+28823485 | MS.gene49596:CDS |
| GAAAGAGAGAAAGGGTCAAA+TGG | 0.390959 | 5.1:-28823278 | None:intergenic |
| CACCTCCTTTCAGAGTTTCA+CGG | 0.398502 | 5.1:-28827723 | None:intergenic |
| CAACTAACCTCATAATTTCC+AGG | 0.404914 | 5.1:-28830697 | None:intergenic |
| AATACTTCGGAAGCAATATT+AGG | 0.407953 | 5.1:-28829791 | None:intergenic |
| AAATACAGAAATAGTTCTTT+CGG | 0.409058 | 5.1:+28824770 | MS.gene49596:CDS |
| GCCAAGTTGGCAGTGTTAGA+AGG | 0.411937 | 5.1:+28829571 | MS.gene49596:CDS |
| AGCAAAGCTTTCTGATGCTT+GGG | 0.414113 | 5.1:+28829495 | MS.gene49596:CDS |
| GTTGATTAGAGGTGGGAGAG+TGG | 0.420107 | 5.1:+28823557 | MS.gene49596:CDS |
| TTTGACACGGTGTAAGGATT+GGG | 0.420481 | 5.1:+28823449 | MS.gene49596:CDS |
| TAGCAAAGCTTTCTGATGCT+TGG | 0.420489 | 5.1:+28829494 | MS.gene49596:CDS |
| AGTTCATTGTTGACGGGGAA+TGG | 0.421692 | 5.1:+28830801 | MS.gene49596:CDS |
| GGAATGTAGCTAAGGCTTCT+GGG | 0.421869 | 5.1:+28823673 | MS.gene49596:CDS |
| TCAAAAGTTTCAAAGCTTTG+TGG | 0.424632 | 5.1:-28829748 | None:intergenic |
| AGTTGGATTGATCAGAGAAC+TGG | 0.425483 | 5.1:+28827578 | MS.gene49596:CDS |
| AGTTCTTTCGGACTTGGATT+CGG | 0.426980 | 5.1:+28824782 | MS.gene49596:CDS |
| GATATAGAAGCTGAGGAGTC+TGG | 0.427099 | 5.1:+28824719 | MS.gene49596:CDS |
| TCCTTCTAACACTGCCAACT+TGG | 0.428742 | 5.1:-28829572 | None:intergenic |
| AAGGTGGGTGGTTATCTTAT+GGG | 0.429905 | 5.1:+28823604 | MS.gene49596:CDS |
| TCTCTGTTTATAGGCTCGTC+AGG | 0.435029 | 5.1:-28827793 | None:intergenic |
| CTGATTCCAGAAGCTGAATA+CGG | 0.436238 | 5.1:-28827873 | None:intergenic |
| CAGATCAAGTTCATTGTTGA+CGG | 0.437492 | 5.1:+28830794 | MS.gene49596:intron |
| GAGCCTATAAACAGAGAAAC+AGG | 0.440505 | 5.1:+28827799 | MS.gene49596:CDS |
| ATTGTCTACTACTATTGATA+AGG | 0.443311 | 5.1:-28827439 | None:intergenic |
| ACACTCAAAAGGGTTTGTAT+TGG | 0.447831 | 5.1:+28823412 | MS.gene49596:CDS |
| TATGGGTGGAATTTGAATCA+TGG | 0.447905 | 5.1:+28823621 | MS.gene49596:CDS |
| CGTTAGCATTGTAACTTCAC+TGG | 0.454556 | 5.1:-28827932 | None:intergenic |
| TGAGGGTATACTAAATCGTC+TGG | 0.458071 | 5.1:+28824745 | MS.gene49596:CDS |
| ATCCAACTTCTCCATGTCTC+TGG | 0.459928 | 5.1:-28827563 | None:intergenic |
| GCTGAGGAGTCTGGCATTGA+GGG | 0.460049 | 5.1:+28824728 | MS.gene49596:CDS |
| GGTTGATGAGACAAAGATTG+AGG | 0.461343 | 5.1:-28823301 | None:intergenic |
| AAGATGGAGAAATCAAATAC+TGG | 0.463137 | 5.1:+28830646 | MS.gene49596:CDS |
| ACCTCCTTTCAGAGTTTCAC+GGG | 0.463569 | 5.1:-28827722 | None:intergenic |
| AAGGAAGAGTTGATTAGAGG+TGG | 0.464944 | 5.1:+28823549 | MS.gene49596:CDS |
| ACAAAGGAAGAGTTGATTAG+AGG | 0.469468 | 5.1:+28823546 | MS.gene49596:CDS |
| AGCCAGAGACATGGAGAAGT+TGG | 0.471254 | 5.1:+28827561 | MS.gene49596:CDS |
| AGATTGAGGGAAAGAGAGAA+AGG | 0.478954 | 5.1:-28823287 | None:intergenic |
| GTTGATGAGACAAAGATTGA+GGG | 0.483793 | 5.1:-28823300 | None:intergenic |
| ATTTGACGGCTGGTCTTCCC+AGG | 0.483924 | 5.1:+28829826 | MS.gene49596:CDS |
| GGATTCGGAGAGAAAGAAGT+TGG | 0.492506 | 5.1:+28824797 | MS.gene49596:CDS |
| AGATCAAGTTCATTGTTGAC+GGG | 0.492824 | 5.1:+28830795 | MS.gene49596:intron |
| GTTGAAGCTATTGTGAAAGA+AGG | 0.493628 | 5.1:+28823585 | MS.gene49596:CDS |
| TAGTAGGATCATTTGACGGC+TGG | 0.500475 | 5.1:+28829816 | MS.gene49596:CDS |
| ATGGGTGGAATTTGAATCAT+GGG | 0.502752 | 5.1:+28823622 | MS.gene49596:CDS |
| AGGAAGAGTTGATTAGAGGT+GGG | 0.503229 | 5.1:+28823550 | MS.gene49596:CDS |
| CATTGCATTGCAGAGAAAGA+TGG | 0.506836 | 5.1:+28830630 | MS.gene49596:intron |
| AGCTGAGGAGTCTGGCATTG+AGG | 0.520081 | 5.1:+28824727 | MS.gene49596:CDS |
| GAAAATTGATCCATTGCGTC+CGG | 0.525932 | 5.1:+28830823 | MS.gene49596:CDS |
| TGAAGATGGGAATGTAGCTA+AGG | 0.526211 | 5.1:+28823665 | MS.gene49596:CDS |
| GTTAGAAGGAAAGATGTCAT+TGG | 0.527307 | 5.1:+28829585 | MS.gene49596:CDS |
| GATCATAGAACCATCAAAGA+TGG | 0.532293 | 5.1:+28824857 | MS.gene49596:CDS |
| TCTACTACTATTGATAAGGT+TGG | 0.533867 | 5.1:-28827435 | None:intergenic |
| CGACTTGAAATCATCAAAAG+AGG | 0.537750 | 5.1:+28827763 | MS.gene49596:CDS |
| TCCAGAAAGGTTTCCTACAA+AGG | 0.544066 | 5.1:+28823530 | MS.gene49596:CDS |
| TTTGATCTGGGAACCACTGA+GGG | 0.544333 | 5.1:-28827486 | None:intergenic |
| TTGTTAACCACCGGACGCAA+TGG | 0.544500 | 5.1:-28830833 | None:intergenic |
| GTGTTGATCAAGTTTGATCT+GGG | 0.550546 | 5.1:-28827498 | None:intergenic |
| AAGAGAGAAAGGGTCAAATG+GGG | 0.553673 | 5.1:-28823276 | None:intergenic |
| TGATTCCAGAAGCTGAATAC+GGG | 0.558491 | 5.1:-28827872 | None:intergenic |
| TAGCCATTGTTGTTAACCAC+CGG | 0.560908 | 5.1:-28830842 | None:intergenic |
| GATTGAGGGAAAGAGAGAAA+GGG | 0.565149 | 5.1:-28823286 | None:intergenic |
| ACGTTCGTTACGTGCCAAGT+TGG | 0.571317 | 5.1:+28829558 | MS.gene49596:CDS |
| GCTTGTGGTTCGGTTCAATG+AGG | 0.577033 | 5.1:-28827462 | None:intergenic |
| AGAGAGAAAGGGTCAAATGG+GGG | 0.577333 | 5.1:-28823275 | None:intergenic |
| GCTGTATCCTGGAAATTATG+AGG | 0.585979 | 5.1:+28830690 | MS.gene49596:CDS |
| CCTCTGTACTTCATTCGTTG+AGG | 0.588567 | 5.1:+28827902 | MS.gene49596:CDS |
| GAAGCTATTGTGAAAGAAGG+TGG | 0.589155 | 5.1:+28823588 | MS.gene49596:CDS |
| AATCCTCACACTGATTCAGC+TGG | 0.595907 | 5.1:-28823747 | None:intergenic |
| AATTGATCCATTGCGTCCGG+TGG | 0.599116 | 5.1:+28830826 | MS.gene49596:CDS |
| GTGGGTGGTTATCTTATGGG+TGG | 0.603777 | 5.1:+28823607 | MS.gene49596:CDS |
| GGGGAAAGTGAGAGAATGCA+TGG | 0.604674 | 5.1:-28823256 | None:intergenic |
| AAGCTATTGTGAAAGAAGGT+GGG | 0.607025 | 5.1:+28823589 | MS.gene49596:CDS |
| TGATCTGGGAACCACTGAGG+GGG | 0.608699 | 5.1:-28827484 | None:intergenic |
| ACTCAATCAAGCCAGAGACA+TGG | 0.613472 | 5.1:+28827552 | MS.gene49596:CDS |
| AAACAGGGATATAGAAGCTG+AGG | 0.619435 | 5.1:+28824712 | MS.gene49596:intron |
| CGTCCGGTGGTTAACAACAA+TGG | 0.624388 | 5.1:+28830839 | MS.gene49596:CDS |
| GAATGTAGCTAAGGCTTCTG+GGG | 0.634417 | 5.1:+28823674 | MS.gene49596:CDS |
| CAGTGAAGTTACAATGCTAA+CGG | 0.643771 | 5.1:+28827933 | MS.gene49596:CDS |
| GAATTCGTATGATTCGTCAT+CGG | 0.647447 | 5.1:+28823710 | MS.gene49596:CDS |
| GTTTGATCTGGGAACCACTG+AGG | 0.648609 | 5.1:-28827487 | None:intergenic |
| AGAATTCGGAGAATCCAGAA+AGG | 0.648857 | 5.1:+28823517 | MS.gene49596:CDS |
| CCCCGTGAAACTCTGAAAGG+AGG | 0.652678 | 5.1:+28827721 | MS.gene49596:CDS |
| GATCAAGTTCATTGTTGACG+GGG | 0.653640 | 5.1:+28830796 | MS.gene49596:intron |
| CAGATCAAACTTGATCAACA+CGG | 0.659066 | 5.1:+28827500 | MS.gene49596:CDS |
| TTAGGCCAAACTACACAAGT+CGG | 0.669876 | 5.1:-28829773 | None:intergenic |
| CTATTGTGAAAGAAGGTGGG+TGG | 0.673848 | 5.1:+28823592 | MS.gene49596:CDS |
| GTTCCCCGTGAAACTCTGAA+AGG | 0.681257 | 5.1:+28827718 | MS.gene49596:CDS |
| TTGATCTGGGAACCACTGAG+GGG | 0.685737 | 5.1:-28827485 | None:intergenic |
| CCTCCTTTCAGAGTTTCACG+GGG | 0.689583 | 5.1:-28827721 | None:intergenic |
| CCTCAACGAATGAAGTACAG+AGG | 0.710308 | 5.1:-28827902 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 28823258 | 28830895 | 28823258 | ID=MS.gene49596 |
| chr5.1 | mRNA | 28823258 | 28830895 | 28823258 | ID=MS.gene49596.t1;Parent=MS.gene49596 |
| chr5.1 | exon | 28823258 | 28823760 | 28823258 | ID=MS.gene49596.t1.exon1;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28823258 | 28823760 | 28823258 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28824718 | 28824880 | 28824718 | ID=MS.gene49596.t1.exon2;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28824718 | 28824880 | 28824718 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28827428 | 28827623 | 28827428 | ID=MS.gene49596.t1.exon3;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28827428 | 28827623 | 28827428 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28827707 | 28827954 | 28827707 | ID=MS.gene49596.t1.exon4;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28827707 | 28827954 | 28827707 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28829469 | 28829614 | 28829469 | ID=MS.gene49596.t1.exon5;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28829469 | 28829614 | 28829469 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28829700 | 28829847 | 28829700 | ID=MS.gene49596.t1.exon6;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28829700 | 28829847 | 28829700 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28830643 | 28830711 | 28830643 | ID=MS.gene49596.t1.exon7;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28830643 | 28830711 | 28830643 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| chr5.1 | exon | 28830797 | 28830895 | 28830797 | ID=MS.gene49596.t1.exon8;Parent=MS.gene49596.t1 |
| chr5.1 | CDS | 28830797 | 28830895 | 28830797 | ID=cds.MS.gene49596.t1;Parent=MS.gene49596.t1 |
| Gene Sequence |
| Protein sequence |
