Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49618.t1 | XP_003613528.1 | 99 | 96 | 1 | 0 | 5 | 100 | 166 | 261 | 2.40E-43 | 184.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49618.t1 | G7K8D4 | 99.0 | 96 | 1 | 0 | 5 | 100 | 166 | 261 | 1.7e-43 | 184.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057618 | MS.gene49618 | 0.930881 | 8.57E-94 | -1.69E-46 |
| MS.gene058171 | MS.gene49618 | 0.807418 | 5.11E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene49618 | 0.804809 | 1.81E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49618.t1 | MTR_5g037700 | 98.958 | 96 | 1 | 0 | 5 | 100 | 166 | 261 | 2.75e-63 | 192 |
| MS.gene49618.t1 | MTR_5g037710 | 84.146 | 82 | 13 | 0 | 5 | 86 | 161 | 242 | 1.75e-42 | 139 |
| MS.gene49618.t1 | MTR_5g037650 | 78.652 | 89 | 19 | 0 | 5 | 93 | 166 | 254 | 2.38e-40 | 134 |
| MS.gene49618.t1 | MTR_5g037630 | 81.395 | 86 | 16 | 0 | 5 | 90 | 166 | 251 | 7.75e-40 | 133 |
| MS.gene49618.t1 | MTR_8g470390 | 63.636 | 88 | 32 | 0 | 5 | 92 | 163 | 250 | 3.50e-24 | 92.4 |
| MS.gene49618.t1 | MTR_8g469680 | 58.621 | 87 | 36 | 0 | 6 | 92 | 203 | 289 | 8.17e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCCTCATACTTACAAATT+TGG | 0.175795 | 5.1:+28412615 | None:intergenic |
| ATCTAGTACATTAATCTAAA+AGG | 0.183870 | 5.1:+28412415 | None:intergenic |
| ACTTACAAATTTGGTGATCT+AGG | 0.360019 | 5.1:+28412624 | None:intergenic |
| ACGTATCACAGAACTTAAAA+TGG | 0.368676 | 5.1:-28412516 | MS.gene49618:CDS |
| TCTAGTACATTAATCTAAAA+GGG | 0.439493 | 5.1:+28412416 | None:intergenic |
| TCACCAAATTTGTAAGTATG+AGG | 0.471914 | 5.1:-28412618 | MS.gene49618:CDS |
| GTTTGAGTACAGGACAGAAC+TGG | 0.483600 | 5.1:-28412708 | MS.gene49618:CDS |
| ATTAGTTGAGGAAATTGCTA+TGG | 0.510520 | 5.1:-28412684 | MS.gene49618:CDS |
| AAGATTAATCGAGTATATGT+TGG | 0.562322 | 5.1:-28412650 | MS.gene49618:CDS |
| GTTATGCTTGTTCAAATCAA+CGG | 0.564491 | 5.1:+28412550 | None:intergenic |
| GACAGAACTGGAATTAGTTG+AGG | 0.600512 | 5.1:-28412696 | MS.gene49618:CDS |
| AGATTTAGAGAACTCAAACA+AGG | 0.649542 | 5.1:-28412450 | MS.gene49618:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGATTAATCGAGTATATGT+TGG | - | chr5.1:28412490-28412509 | MS.gene49618:CDS | 25.0% |
| ACGTATCACAGAACTTAAAA+TGG | - | chr5.1:28412624-28412643 | MS.gene49618:CDS | 30.0% | |
| AGATTTAGAGAACTCAAACA+AGG | - | chr5.1:28412690-28412709 | MS.gene49618:CDS | 30.0% | |
| ATTAGTTGAGGAAATTGCTA+TGG | - | chr5.1:28412456-28412475 | MS.gene49618:CDS | 30.0% | |
| GTTATGCTTGTTCAAATCAA+CGG | + | chr5.1:28412593-28412612 | None:intergenic | 30.0% | |
| GTTCCTCATACTTACAAATT+TGG | + | chr5.1:28412528-28412547 | None:intergenic | 30.0% | |
| TCACCAAATTTGTAAGTATG+AGG | - | chr5.1:28412522-28412541 | MS.gene49618:CDS | 30.0% | |
| ! | ACTTACAAATTTGGTGATCT+AGG | + | chr5.1:28412519-28412538 | None:intergenic | 30.0% |
| GACAGAACTGGAATTAGTTG+AGG | - | chr5.1:28412444-28412463 | MS.gene49618:CDS | 40.0% | |
| GTTTGAGTACAGGACAGAAC+TGG | - | chr5.1:28412432-28412451 | MS.gene49618:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 28412430 | 28412732 | 28412430 | ID=MS.gene49618 |
| chr5.1 | mRNA | 28412430 | 28412732 | 28412430 | ID=MS.gene49618.t1;Parent=MS.gene49618 |
| chr5.1 | exon | 28412430 | 28412732 | 28412430 | ID=MS.gene49618.t1.exon1;Parent=MS.gene49618.t1 |
| chr5.1 | CDS | 28412430 | 28412732 | 28412430 | ID=cds.MS.gene49618.t1;Parent=MS.gene49618.t1 |
| Gene Sequence |
| Protein sequence |
