Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49704.t1 | XP_013446887.1 | 97.9 | 916 | 16 | 1 | 1 | 916 | 1 | 913 | 0.00E+00 | 1209.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49704.t1 | Q0WQ57 | 48.3 | 983 | 349 | 27 | 1 | 916 | 1 | 891 | 1.1e-85 | 319.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49704.t1 | A0A072TTU0 | 97.9 | 916 | 16 | 1 | 1 | 916 | 1 | 913 | 0.0e+00 | 1209.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057682 | MS.gene49704 | 0.816781 | 4.63E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49704.t1 | MTR_8g494210 | 98.690 | 916 | 9 | 1 | 1 | 916 | 1 | 913 | 0.0 | 1816 |
| MS.gene49704.t1 | MTR_8g494210 | 98.421 | 760 | 9 | 1 | 1 | 760 | 1 | 757 | 0.0 | 1483 |
| MS.gene49704.t1 | MTR_3g096790 | 77.358 | 318 | 61 | 3 | 610 | 916 | 355 | 672 | 5.74e-125 | 393 |
| MS.gene49704.t1 | MTR_3g096790 | 62.393 | 234 | 80 | 4 | 201 | 426 | 53 | 286 | 2.09e-66 | 235 |
| MS.gene49704.t1 | MTR_3g096790 | 77.358 | 318 | 61 | 3 | 610 | 916 | 418 | 735 | 7.56e-125 | 395 |
| MS.gene49704.t1 | MTR_3g096790 | 54.799 | 323 | 129 | 6 | 120 | 426 | 28 | 349 | 1.78e-75 | 262 |
| MS.gene49704.t1 | MTR_3g088495 | 60.408 | 245 | 83 | 3 | 684 | 915 | 277 | 520 | 4.34e-81 | 272 |
| MS.gene49704.t1 | MTR_8g077150 | 51.187 | 379 | 135 | 9 | 561 | 914 | 1087 | 1440 | 2.28e-79 | 282 |
| MS.gene49704.t1 | MTR_8g077150 | 51.093 | 366 | 133 | 9 | 561 | 904 | 1087 | 1428 | 7.38e-74 | 266 |
| MS.gene49704.t1 | MTR_1g022415 | 40.654 | 428 | 188 | 8 | 502 | 914 | 991 | 1367 | 4.13e-73 | 263 |
| MS.gene49704.t1 | MTR_8g094000 | 63.830 | 188 | 31 | 4 | 107 | 294 | 50 | 200 | 1.60e-55 | 191 |
| MS.gene49704.t1 | MTR_3g111700 | 64.516 | 93 | 33 | 0 | 819 | 911 | 596 | 688 | 2.78e-37 | 150 |
| MS.gene49704.t1 | MTR_3g111700 | 64.516 | 93 | 33 | 0 | 819 | 911 | 592 | 684 | 2.90e-37 | 150 |
| MS.gene49704.t1 | MTR_3g088495 | 52.874 | 174 | 68 | 3 | 684 | 844 | 277 | 449 | 8.96e-37 | 145 |
| MS.gene49704.t1 | MTR_3g088495 | 52.874 | 174 | 68 | 3 | 684 | 844 | 277 | 449 | 1.01e-36 | 145 |
| MS.gene49704.t1 | MTR_8g079472 | 53.608 | 97 | 45 | 0 | 819 | 915 | 365 | 461 | 3.03e-31 | 129 |
| MS.gene49704.t1 | MTR_2g049770 | 56.701 | 97 | 42 | 0 | 819 | 915 | 357 | 453 | 4.27e-31 | 128 |
| MS.gene49704.t1 | MTR_6g038630 | 80.597 | 67 | 8 | 1 | 96 | 162 | 1 | 62 | 1.36e-25 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49704.t1 | AT4G12770 | 47.336 | 976 | 369 | 31 | 1 | 916 | 1 | 891 | 0.0 | 577 |
| MS.gene49704.t1 | AT4G12770 | 47.282 | 975 | 369 | 31 | 1 | 916 | 1 | 889 | 0.0 | 576 |
| MS.gene49704.t1 | AT4G12780 | 47.256 | 984 | 371 | 32 | 1 | 916 | 1 | 904 | 0.0 | 575 |
| MS.gene49704.t1 | AT4G12780 | 45.935 | 984 | 357 | 30 | 1 | 916 | 1 | 877 | 2.94e-179 | 541 |
| MS.gene49704.t1 | AT4G12780 | 45.155 | 1001 | 357 | 31 | 1 | 916 | 1 | 894 | 1.00e-174 | 530 |
| MS.gene49704.t1 | AT4G12780 | 42.290 | 882 | 361 | 32 | 1 | 814 | 1 | 802 | 2.51e-112 | 364 |
| MS.gene49704.t1 | AT4G12780 | 40.816 | 882 | 347 | 30 | 1 | 814 | 1 | 775 | 4.74e-100 | 330 |
| MS.gene49704.t1 | AT4G12780 | 40.816 | 882 | 347 | 30 | 1 | 814 | 1 | 775 | 4.74e-100 | 330 |
| MS.gene49704.t1 | AT1G21660 | 56.504 | 246 | 90 | 4 | 680 | 915 | 284 | 522 | 7.34e-82 | 274 |
| MS.gene49704.t1 | AT1G21660 | 31.959 | 291 | 125 | 14 | 1 | 265 | 1 | 244 | 8.77e-11 | 65.9 |
| MS.gene49704.t1 | AT4G36520 | 52.431 | 288 | 87 | 7 | 638 | 913 | 1171 | 1420 | 1.26e-73 | 265 |
| MS.gene49704.t1 | AT1G75310 | 43.213 | 361 | 155 | 8 | 576 | 914 | 1118 | 1450 | 6.71e-66 | 242 |
| MS.gene49704.t1 | AT1G75310 | 44.598 | 361 | 147 | 9 | 576 | 914 | 1118 | 1447 | 8.13e-65 | 238 |
| MS.gene49704.t1 | AT1G75100 | 59.783 | 92 | 37 | 0 | 821 | 912 | 556 | 647 | 7.13e-34 | 139 |
| MS.gene49704.t1 | AT1G30280 | 48.352 | 91 | 46 | 1 | 825 | 914 | 361 | 451 | 4.73e-22 | 100 |
| MS.gene49704.t1 | AT1G30280 | 49.383 | 81 | 40 | 1 | 825 | 904 | 361 | 441 | 3.75e-19 | 92.0 |
Find 266 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAAACCACTATTATTATT+TGG | 0.152986 | 4.4:+8059090 | None:intergenic |
| CCCTTCTGCTGTACTTTATC+TGG | 0.178713 | 4.4:+8054547 | None:intergenic |
| GATGATCTTGAATCCTTCTT+TGG | 0.209699 | 4.4:-8058067 | MS.gene49704:CDS |
| CCAAATAATAATAGTGGTTT+TGG | 0.234183 | 4.4:-8059090 | MS.gene49704:CDS |
| CTTCTGCAGCTGCCCTTTCT+CGG | 0.239054 | 4.4:+8058272 | None:intergenic |
| GAAAAGGTAGGCATGTAAAA+TGG | 0.249633 | 4.4:+8059156 | None:intergenic |
| GCTGCTAAAAGCAAAGAATT+TGG | 0.249874 | 4.4:-8058586 | MS.gene49704:CDS |
| CCTATCCATTGCTTCCTTCA+TGG | 0.253657 | 4.4:+8058663 | None:intergenic |
| CTTCTATCTTCTTCCGATTT+CGG | 0.267797 | 4.4:-8061916 | MS.gene49704:CDS |
| AACAGGCATGCTCCTGATAT+TGG | 0.270642 | 4.4:-8059594 | MS.gene49704:intron |
| ACTTCTCTCAACTTCGATTT+CGG | 0.288273 | 4.4:-8061823 | MS.gene49704:CDS |
| GATGATCTTACCTCAATCTT+TGG | 0.292226 | 4.4:-8057588 | MS.gene49704:CDS |
| GACGATCTTCTTGGTGGATT+TGG | 0.293717 | 4.4:-8061490 | MS.gene49704:CDS |
| TTAGGCATGCAAAGGAAGTT+AGG | 0.295874 | 4.4:-8058627 | MS.gene49704:CDS |
| CGGTGGCGGTGAAGAATTTC+CGG | 0.298291 | 4.4:+8061633 | None:intergenic |
| GATGATTTGTTAGCTGGATT+TGG | 0.307679 | 4.4:-8061394 | MS.gene49704:CDS |
| GATTTGGATTCCATGTTTCC+TGG | 0.308043 | 4.4:-8061661 | MS.gene49704:CDS |
| GAATTTGAAGATGATGATTT+AGG | 0.314246 | 4.4:+8061853 | None:intergenic |
| CTGCTGTTAAGAAGGCTTAT+AGG | 0.314547 | 4.4:-8054591 | MS.gene49704:CDS |
| CAGCTCTTTCAGCACGCTCT+CGG | 0.322315 | 4.4:+8058320 | None:intergenic |
| AGGGTCTGTGAAGTTGCTTC+TGG | 0.325149 | 4.4:+8059470 | None:intergenic |
| TCGCGATGCACCCTTCGATT+TGG | 0.328096 | 4.4:-8061677 | MS.gene49704:CDS |
| TCGAAGGGTTCTGGTAGTTC+TGG | 0.353181 | 4.4:-8061460 | MS.gene49704:CDS |
| AGACATATCTGCCGGAATAT+TGG | 0.355042 | 4.4:+8059062 | None:intergenic |
| GGTAAGGATTCGAAGGGTTC+TGG | 0.366296 | 4.4:-8061469 | MS.gene49704:CDS |
| GGTGCATTTGACGATCTTCT+TGG | 0.369941 | 4.4:-8061499 | MS.gene49704:CDS |
| TGCTTCTGGATGAATCCATT+GGG | 0.371684 | 4.4:+8059484 | None:intergenic |
| AGAAGCTGCAGCCCTCTCTC+TGG | 0.377572 | 4.4:+8058117 | None:intergenic |
| CGGAAATTCTTCACCGCCAC+CGG | 0.383257 | 4.4:-8061632 | MS.gene49704:CDS |
| TTCTGCAGCTGCCCTTTCTC+GGG | 0.385416 | 4.4:+8058273 | None:intergenic |
| AAAGAAGATTAATGAAAGAA+AGG | 0.388497 | 4.4:-8058465 | MS.gene49704:CDS |
| AAAAGGCCTTGGAGCACTGC+TGG | 0.390010 | 4.4:+8058030 | None:intergenic |
| ACTGGCTGCCAACCACATTC+AGG | 0.393307 | 4.4:+8054640 | None:intergenic |
| CTGCTAAAAGCAAAGAATTT+GGG | 0.394734 | 4.4:-8058585 | MS.gene49704:CDS |
| TGCATCGCGAGGATTGGATT+TGG | 0.400673 | 4.4:+8061690 | None:intergenic |
| GGTGAAGAGATCGTCGAAAC+TGG | 0.400835 | 4.4:+8061732 | None:intergenic |
| CGTGGAGGAGGTGGTCTTGA+AGG | 0.405564 | 4.4:+8058946 | None:intergenic |
| AGCTCTTTCAGCACGCTCTC+GGG | 0.406825 | 4.4:+8058321 | None:intergenic |
| AAAACATCGTCGTCGTAAAC+CGG | 0.409777 | 4.4:+8061598 | None:intergenic |
| CAGCAGCCCTCTCAGCTCTC+TGG | 0.410280 | 4.4:+8058365 | None:intergenic |
| AAGCAACTTCACAGACCCTT+TGG | 0.414694 | 4.4:-8059466 | MS.gene49704:CDS |
| GAATGGGTCTTCTGCTTCGT+TGG | 0.420693 | 4.4:+8059530 | None:intergenic |
| TCAGAGGACAAACCAATATC+AGG | 0.421078 | 4.4:+8059582 | None:intergenic |
| TCGCCTCCAAATAATAATAG+TGG | 0.421406 | 4.4:-8059096 | MS.gene49704:CDS |
| ATCGGATGTACGAGCGGATC+GGG | 0.421622 | 4.4:+8061798 | None:intergenic |
| AAAATCCCCATCACTTTCAA+TGG | 0.422856 | 4.4:+8059188 | None:intergenic |
| GAAGAATTTCCGGCGGATCC+AGG | 0.423256 | 4.4:+8061643 | None:intergenic |
| GGATGAACCAACAGAAGAAT+GGG | 0.423369 | 4.4:-8058090 | MS.gene49704:CDS |
| GAAGGGTGCATCGCGAGGAT+TGG | 0.424702 | 4.4:+8061684 | None:intergenic |
| CACGGCTGCTGCGGCCATGA+AGG | 0.424999 | 4.4:-8058677 | MS.gene49704:CDS |
| AATGCACCACTATCCGATTT+CGG | 0.430444 | 4.4:+8061514 | None:intergenic |
| AAAAGGTAGGCATGTAAAAT+GGG | 0.431302 | 4.4:+8059157 | None:intergenic |
| CACATGCTGAAGCCCGAGAA+AGG | 0.432511 | 4.4:-8058285 | MS.gene49704:CDS |
| ACTGTGGAGTAGTCCCTAAA+AGG | 0.432654 | 4.4:+8058013 | None:intergenic |
| AAGAAGATTAATGAAAGAAA+GGG | 0.433122 | 4.4:-8058464 | MS.gene49704:CDS |
| TGCAGCCCTCTCTCTGGCCT+CGG | 0.434795 | 4.4:+8058123 | None:intergenic |
| ATCCCAGAAAACTTATCAAC+TGG | 0.435062 | 4.4:+8059243 | None:intergenic |
| GAAGTAGACCTTTGGCCAAC+TGG | 0.437021 | 4.4:+8059117 | None:intergenic |
| ATAGGGCTGAGGCTAAATTT+AGG | 0.438312 | 4.4:-8058645 | MS.gene49704:CDS |
| AGCTGAACGTGCTGCAGAAA+GGG | 0.440378 | 4.4:-8058152 | MS.gene49704:CDS |
| AGCAAAGGAAAGAGCCGAAA+AGG | 0.440956 | 4.4:-8058251 | MS.gene49704:CDS |
| TTGGAGGCGAAGTAGACCTT+TGG | 0.441025 | 4.4:+8059109 | None:intergenic |
| CTTGAATCCTTCTTTGGCGC+AGG | 0.441661 | 4.4:-8058061 | MS.gene49704:CDS |
| TGCAGAAAGGGCTGCTGCCG+AGG | 0.441888 | 4.4:-8058140 | MS.gene49704:CDS |
| TTGAGGTTTGATACCGAAAT+CGG | 0.445371 | 4.4:+8061903 | None:intergenic |
| AAGCAAAGAATTTGGGCAAT+CGG | 0.447137 | 4.4:-8058578 | MS.gene49704:CDS |
| GTTGATGCAAATACTTTGAA+TGG | 0.447847 | 4.4:+8059513 | None:intergenic |
| AGCCGAAAAGGCTGCTGCAG+CGG | 0.449414 | 4.4:-8058239 | MS.gene49704:CDS |
| TTCCCGGTGCTCTTCAGCCC+AGG | 0.449839 | 4.4:+8061565 | None:intergenic |
| AGGATGAACCAACAGAAGAA+TGG | 0.450203 | 4.4:-8058091 | MS.gene49704:CDS |
| CGGTGAACCTGTTCCTGACC+TGG | 0.450442 | 4.4:+8058909 | None:intergenic |
| TACAGCTGCTGCTGTTAAGA+AGG | 0.453611 | 4.4:-8054599 | MS.gene49704:CDS |
| GAAATTAAGCGATGGTCTGC+AGG | 0.454087 | 4.4:-8056174 | MS.gene49704:CDS |
| TGCTGCAGCGGCAAAGGAGA+GGG | 0.454506 | 4.4:-8058227 | MS.gene49704:CDS |
| ATTTACTAATTTCTTCCAAA+GGG | 0.457496 | 4.4:+8059451 | None:intergenic |
| ATTGGCCTTCTTTCTAGTGT+TGG | 0.457603 | 4.4:+8058879 | None:intergenic |
| TCTTGAAACTCTCCGGATGA+TGG | 0.459248 | 4.4:+8056706 | None:intergenic |
| AATAGTTCATCAACTTCAAA+TGG | 0.459870 | 4.4:-8059402 | MS.gene49704:CDS |
| GATCATCCCCATTCTTCTGT+TGG | 0.462204 | 4.4:+8058083 | None:intergenic |
| ATTGGATTTGGAGTTGGATC+CGG | 0.462599 | 4.4:+8061702 | None:intergenic |
| TAGTTCTGGTAGGAAAGGTT+TGG | 0.462986 | 4.4:-8061446 | MS.gene49704:CDS |
| GGAAAGAGAAAAGGCTAGAC+AGG | 0.463810 | 4.4:-8058440 | MS.gene49704:CDS |
| TTTATTAGAATCAGATGAAA+AGG | 0.464167 | 4.4:+8059140 | None:intergenic |
| AAGATTATCGAAATCGTTCA+TGG | 0.464678 | 4.4:+8061936 | None:intergenic |
| AGTAAGGATAACAGTGCTCC+AGG | 0.469085 | 4.4:-8059303 | MS.gene49704:CDS |
| ATATTCACTGGCCGTGGAGG+AGG | 0.470034 | 4.4:+8058934 | None:intergenic |
| TTTGGAGTTGGATCCGGTGG+TGG | 0.470126 | 4.4:+8061708 | None:intergenic |
| GCCACCGGTTTATGATAAAC+CGG | 0.470876 | 4.4:-8061617 | MS.gene49704:CDS |
| CGGCAAAGGAGAGGGAAGCA+CGG | 0.474267 | 4.4:-8058219 | MS.gene49704:CDS |
| GAGCTGAGAGGGCTGCTGTT+GGG | 0.474704 | 4.4:-8058360 | MS.gene49704:CDS |
| TTGCTTCTGGATGAATCCAT+TGG | 0.475210 | 4.4:+8059483 | None:intergenic |
| TTCAACTTCTTGAAACTCTC+CGG | 0.475999 | 4.4:+8056699 | None:intergenic |
| TTCTCGTGTGGCTCTTTCTA+CGG | 0.477359 | 4.4:+8058417 | None:intergenic |
| TAGGCATGCAAAGGAAGTTA+GGG | 0.478161 | 4.4:-8058626 | MS.gene49704:CDS |
| TGGAATCATGTGAGGATGTA+TGG | 0.478218 | 4.4:-8059020 | MS.gene49704:CDS |
| GGAGGATCCGAAAACGGAAT+CGG | 0.478568 | 4.4:+8061780 | None:intergenic |
| TCCAAAATATGAAGAAAACT+TGG | 0.480365 | 4.4:-8059040 | MS.gene49704:CDS |
| TTCACTGGCCGTGGAGGAGG+TGG | 0.480599 | 4.4:+8058937 | None:intergenic |
| AAAGGCTGCTGCAGCGGCAA+AGG | 0.483077 | 4.4:-8058233 | MS.gene49704:CDS |
| TCTTCTTGGTGGATTTGGTA+AGG | 0.484525 | 4.4:-8061485 | MS.gene49704:CDS |
| TTTGAAGCTGCTCCATCATC+CGG | 0.484991 | 4.4:-8056718 | MS.gene49704:intron |
| GCGAGGATTGGATTTGGAGT+TGG | 0.486697 | 4.4:+8061696 | None:intergenic |
| GTGCCTGGGCTGAAGAGCAC+CGG | 0.487604 | 4.4:-8061568 | MS.gene49704:CDS |
| GTGTATTGATTTCAGAGGCT+TGG | 0.487654 | 4.4:-8056213 | MS.gene49704:intron |
| AATATTCCCAGGTCAGGAAC+AGG | 0.488483 | 4.4:-8058916 | MS.gene49704:CDS |
| TTGATGCAAATACTTTGAAT+GGG | 0.490120 | 4.4:+8059514 | None:intergenic |
| TCTGGTAGTTCTGGTAGGAA+AGG | 0.491013 | 4.4:-8061451 | MS.gene49704:CDS |
| GGAACGCCACCAGAGGTCTC+AGG | 0.493525 | 4.4:-8056644 | MS.gene49704:CDS |
| GAGTAGTCCCTAAAAGGCCT+TGG | 0.493856 | 4.4:+8058019 | None:intergenic |
| GTCGCGGTTGTGACGGTTGG+TGG | 0.493959 | 4.4:+8061759 | None:intergenic |
| AGAAAGGGAGGAAAGAGAAA+AGG | 0.494730 | 4.4:-8058449 | MS.gene49704:CDS |
| GATCTTACCTCAATCTTTGG+AGG | 0.495365 | 4.4:-8057585 | MS.gene49704:intron |
| GGCAAAGGAGAGGGAAGCAC+GGG | 0.495927 | 4.4:-8058218 | MS.gene49704:CDS |
| TGGGTCTTCTGCTTCGTTGG+AGG | 0.496130 | 4.4:+8059533 | None:intergenic |
| AGTTCATCAAACTGAGATGT+TGG | 0.497722 | 4.4:+8058781 | None:intergenic |
| AAATCCCCATCACTTTCAAT+GGG | 0.501076 | 4.4:+8059189 | None:intergenic |
| GGAGAGTTTCAAGAAGTTGA+AGG | 0.502098 | 4.4:-8056697 | MS.gene49704:CDS |
| TTTCTAGTGTTGGTAGAAGC+CGG | 0.502574 | 4.4:+8058889 | None:intergenic |
| TTCATCTCTAACTCATCATC+GGG | 0.502847 | 4.4:+8058700 | None:intergenic |
| AAGCTGAACGTGCTGCAGAA+AGG | 0.503734 | 4.4:-8058153 | MS.gene49704:CDS |
| CGGGAAAGAAGAAAATTCAT+TGG | 0.506491 | 4.4:+8058861 | None:intergenic |
| GATCGCCCGAGCCCATCAAA+AGG | 0.507618 | 4.4:+8059357 | None:intergenic |
| GGAGTTGGATCCGGTGGTGG+TGG | 0.507911 | 4.4:+8061711 | None:intergenic |
| ACATTCCCATTGAAAGTGAT+GGG | 0.508982 | 4.4:-8059194 | MS.gene49704:CDS |
| AAACCACTATTATTATTTGG+AGG | 0.509321 | 4.4:+8059093 | None:intergenic |
| CTGCTGCAGCGGCAAAGGAG+AGG | 0.509413 | 4.4:-8058228 | MS.gene49704:CDS |
| TCCTGAGAGGCACTCACAAA+AGG | 0.509893 | 4.4:-8059217 | MS.gene49704:CDS |
| ACATGCTGAAGCCCGAGAAA+GGG | 0.510189 | 4.4:-8058284 | MS.gene49704:CDS |
| GATTTAGGTGGTTGAGACAT+TGG | 0.513013 | 4.4:+8061868 | None:intergenic |
| GTTGGTGGAGGATCCGAAAA+CGG | 0.513829 | 4.4:+8061774 | None:intergenic |
| CTGGGAATATTCACTGGCCG+TGG | 0.514534 | 4.4:+8058928 | None:intergenic |
| AATTTACTAATTTCTTCCAA+AGG | 0.514675 | 4.4:+8059450 | None:intergenic |
| TGAAAGAGCTGCAGTGCAAA+GGG | 0.515365 | 4.4:-8058308 | MS.gene49704:CDS |
| TGCTGCCGAGGCCAGAGAGA+GGG | 0.515569 | 4.4:-8058128 | MS.gene49704:CDS |
| TGGGCTCGGGCGATCTGTAC+CGG | 0.517078 | 4.4:-8059349 | MS.gene49704:CDS |
| CCAGTGAATATTCCCAGGTC+AGG | 0.519832 | 4.4:-8058922 | MS.gene49704:CDS |
| TCTTGAACCTGCGCCAAAGA+AGG | 0.519881 | 4.4:+8058054 | None:intergenic |
| GGAGTCGCGGTTGTGACGGT+TGG | 0.521276 | 4.4:+8061756 | None:intergenic |
| CCTGACCTGGGAATATTCAC+TGG | 0.521454 | 4.4:+8058922 | None:intergenic |
| CCATGAAGGAAGCAATGGAT+AGG | 0.521468 | 4.4:-8058663 | MS.gene49704:CDS |
| CTGAAAGAGCTGCAGTGCAA+AGG | 0.523551 | 4.4:-8058309 | MS.gene49704:CDS |
| CAGGTCAGGAACAGGTTCAC+CGG | 0.523999 | 4.4:-8058908 | MS.gene49704:CDS |
| CTGCTGCCGAGGCCAGAGAG+AGG | 0.524296 | 4.4:-8058129 | MS.gene49704:CDS |
| AAGGGCAGCTGCAGAAGCAA+AGG | 0.525358 | 4.4:-8058266 | MS.gene49704:CDS |
| GTCTGCTACAGCTGCAGCAT+CGG | 0.526007 | 4.4:-8058809 | MS.gene49704:CDS |
| AAGATTAATGAAAGAAAGGG+AGG | 0.527517 | 4.4:-8058461 | MS.gene49704:CDS |
| CTTGTGCTTGTGTTCAACCC+TGG | 0.527896 | 4.4:+8059285 | None:intergenic |
| TGCATCAACTTCAGCCCCAA+TGG | 0.528165 | 4.4:-8059499 | MS.gene49704:CDS |
| GCCTCGCCGAAATCGGATAG+TGG | 0.528352 | 4.4:-8061520 | MS.gene49704:CDS |
| ATCAAATCAGTTAAACCGAC+TGG | 0.528472 | 4.4:+8054622 | None:intergenic |
| CGATGGTCTGCAGGAAAAGA+GGG | 0.530420 | 4.4:-8056165 | MS.gene49704:CDS |
| TCCATGTTTCCTGGATCCGC+CGG | 0.532234 | 4.4:-8061652 | MS.gene49704:CDS |
| GTTCATCTCTAACTCATCAT+CGG | 0.536958 | 4.4:+8058699 | None:intergenic |
| GTAAATCATCGTCTAGTGAC+AGG | 0.537836 | 4.4:-8061366 | MS.gene49704:intron |
| GAGAGAATCGAGTGGAACCC+GGG | 0.537935 | 4.4:+8058842 | None:intergenic |
| AGCACGCCAGAGAGCTGAGA+GGG | 0.538752 | 4.4:-8058371 | MS.gene49704:CDS |
| TGCTGTGGCCTGAATGTGGT+TGG | 0.539341 | 4.4:-8054648 | MS.gene49704:CDS |
| AACACAAGCACAAGTTGGAC+TGG | 0.539509 | 4.4:-8059276 | MS.gene49704:CDS |
| AATGATGCTGAGAGTGGAAA+TGG | 0.540517 | 4.4:-8058727 | MS.gene49704:CDS |
| GCATTTGACGATCTTCTTGG+TGG | 0.542044 | 4.4:-8061496 | MS.gene49704:CDS |
| TCCAGATAAAGTACAGCAGA+AGG | 0.543113 | 4.4:-8054548 | MS.gene49704:CDS |
| TTGAAGGGGAAAGTGAAGAA+AGG | 0.545134 | 4.4:-8056681 | MS.gene49704:CDS |
| GCGATGGTCTGCAGGAAAAG+AGG | 0.545276 | 4.4:-8056166 | MS.gene49704:CDS |
| TTCTACCAACACTAGAAAGA+AGG | 0.545550 | 4.4:-8058884 | MS.gene49704:CDS |
| TCAACAATATTACTTGATGA+TGG | 0.546732 | 4.4:+8057609 | None:intergenic |
| TGAGAGAATCGAGTGGAACC+CGG | 0.546757 | 4.4:+8058841 | None:intergenic |
| GAGAGTTTCAAGAAGTTGAA+GGG | 0.547918 | 4.4:-8056696 | MS.gene49704:CDS |
| GGAGGTGGTCTTGAAGGCGG+TGG | 0.549807 | 4.4:+8058952 | None:intergenic |
| GCTGCAGCTGTAGCAGACCT+AGG | 0.550205 | 4.4:+8058814 | None:intergenic |
| AGAGCTGAGAGGGCTGCTGT+TGG | 0.550262 | 4.4:-8058361 | MS.gene49704:CDS |
| TCTGATTCTAATAAACCAGT+TGG | 0.550868 | 4.4:-8059132 | MS.gene49704:CDS |
| AAACCTACCTCCAAAGATTG+AGG | 0.552663 | 4.4:+8057578 | None:intergenic |
| GACATTCCCATTGAAAGTGA+TGG | 0.553237 | 4.4:-8059195 | MS.gene49704:CDS |
| TCAGAGGCTTGGTGAAACAC+TGG | 0.553477 | 4.4:-8056202 | MS.gene49704:intron |
| TGGATTTGGTAAGGATTCGA+AGG | 0.554191 | 4.4:-8061476 | MS.gene49704:CDS |
| AATCGGATGTACGAGCGGAT+CGG | 0.556082 | 4.4:+8061797 | None:intergenic |
| AAAGGCCTTGGAGCACTGCT+GGG | 0.557735 | 4.4:+8058031 | None:intergenic |
| TTTGTACTAGGCAAAAGCAT+TGG | 0.557931 | 4.4:-8056483 | MS.gene49704:intron |
| GGAAAGAGCTACAGCTGCGA+GGG | 0.561717 | 4.4:-8058197 | MS.gene49704:CDS |
| CCACACGCTCCTGAGACCTC+TGG | 0.562188 | 4.4:+8056635 | None:intergenic |
| TAAACCGGTTTATCATAAAC+CGG | 0.562418 | 4.4:+8061613 | None:intergenic |
| GCGGTTGTGACGGTTGGTGG+AGG | 0.566011 | 4.4:+8061762 | None:intergenic |
| AGAAAGGCGAAAAGCTAGGT+TGG | 0.567707 | 4.4:-8056665 | MS.gene49704:CDS |
| TTAGAATCAGATGAAAAGGT+AGG | 0.568900 | 4.4:+8059144 | None:intergenic |
| ATCTGAGAGAAATAGCAGTA+AGG | 0.569026 | 4.4:-8059319 | MS.gene49704:CDS |
| CATGAAGGAAGCAATGGATA+GGG | 0.569681 | 4.4:-8058662 | MS.gene49704:CDS |
| CGAGGGCTGAAACTGAAGCA+CGG | 0.569761 | 4.4:-8058180 | MS.gene49704:CDS |
| CTCGATTCTCTCATGACCCT+AGG | 0.569854 | 4.4:-8058831 | MS.gene49704:CDS |
| TGGAGGAGTCGCGGTTGTGA+CGG | 0.570460 | 4.4:+8061752 | None:intergenic |
| GGAAGCAATGGATAGGGCTG+AGG | 0.570749 | 4.4:-8058656 | MS.gene49704:CDS |
| CACGGCCAGTGAATATTCCC+AGG | 0.573443 | 4.4:-8058927 | MS.gene49704:CDS |
| TATCTGCCGGAATATTGGCT+TGG | 0.575063 | 4.4:+8059067 | None:intergenic |
| GTGAAGAAAGGCGAAAAGCT+AGG | 0.575378 | 4.4:-8056669 | MS.gene49704:CDS |
| AGAGATGAACACGGCTGCTG+CGG | 0.576590 | 4.4:-8058686 | MS.gene49704:CDS |
| AAACATGGAATCCAAATCGA+AGG | 0.578717 | 4.4:+8061666 | None:intergenic |
| GGTGAACCTGTTCCTGACCT+GGG | 0.579607 | 4.4:+8058910 | None:intergenic |
| TGCCTGGGCTGAAGAGCACC+GGG | 0.580373 | 4.4:-8061567 | MS.gene49704:CDS |
| ATCGCCCGAGCCCATCAAAA+GGG | 0.580965 | 4.4:+8059358 | None:intergenic |
| TGCACTTACAGTTGGGTCAG+AGG | 0.581383 | 4.4:+8059566 | None:intergenic |
| GATCTCTTCACCACCACCAC+CGG | 0.582900 | 4.4:-8061721 | MS.gene49704:CDS |
| GGATTTGGAGTTGGATCCGG+TGG | 0.584491 | 4.4:+8061705 | None:intergenic |
| AAGAAAGGTTAGAACGTGAC+CGG | 0.584639 | 4.4:-8058516 | MS.gene49704:CDS |
| AAATCGAAGGGTGCATCGCG+AGG | 0.586156 | 4.4:+8061679 | None:intergenic |
| GGAGGAGGTGGTCTTGAAGG+CGG | 0.586321 | 4.4:+8058949 | None:intergenic |
| CACGGTAATGATGCTGAGAG+TGG | 0.586656 | 4.4:-8058733 | MS.gene49704:CDS |
| AAAGGTGTTCGACCTACTCA+AGG | 0.588294 | 4.4:-8054494 | MS.gene49704:intron |
| TTTGCTGCAGGCAGTAGTCA+CGG | 0.590352 | 4.4:-8058751 | MS.gene49704:CDS |
| GAAGGCGGTGGAGCAGTGGT+TGG | 0.591573 | 4.4:+8058964 | None:intergenic |
| CTAAGTACATACATATTGCA+AGG | 0.591810 | 4.4:+8056119 | None:intergenic |
| GAGGGCTGAAACTGAAGCAC+GGG | 0.592338 | 4.4:-8058179 | MS.gene49704:CDS |
| GGTTGAACACAAGCACAAGT+TGG | 0.595734 | 4.4:-8059281 | MS.gene49704:CDS |
| CTAATAAACCAGTTGGCCAA+AGG | 0.597078 | 4.4:-8059125 | MS.gene49704:CDS |
| AGAGGGCTGCAGCTTCTGCA+AGG | 0.597470 | 4.4:-8058111 | MS.gene49704:CDS |
| TTCAGGAGGCAGATGCAACA+AGG | 0.599208 | 4.4:-8057662 | MS.gene49704:intron |
| GGCTAAATTTAGGCATGCAA+AGG | 0.599654 | 4.4:-8058635 | MS.gene49704:CDS |
| GAATGTGGTTGGCAGCCAGT+CGG | 0.601721 | 4.4:-8054637 | MS.gene49704:CDS |
| CAGGTGCTGTGGCCTGAATG+TGG | 0.603624 | 4.4:-8054652 | MS.gene49704:CDS |
| GGAATATTCACTGGCCGTGG+AGG | 0.605353 | 4.4:+8058931 | None:intergenic |
| CACGCTCCTGAGACCTCTGG+TGG | 0.605546 | 4.4:+8056638 | None:intergenic |
| CAAGACCCAGCAGTGCTCCA+AGG | 0.607904 | 4.4:-8058036 | MS.gene49704:CDS |
| GGATTTGGTAAGGATTCGAA+GGG | 0.608506 | 4.4:-8061475 | MS.gene49704:CDS |
| CAAAGATTAAAACAGCTCCT+CGG | 0.608998 | 4.4:+8053678 | None:intergenic |
| CTGCAGCTGTAGCAGACCTA+GGG | 0.609563 | 4.4:+8058815 | None:intergenic |
| TTTCTCTCAGATGAGAATGC+CGG | 0.610142 | 4.4:+8059330 | None:intergenic |
| ATTGGAGCGGATTTACCTTG+AGG | 0.613326 | 4.4:+8061886 | None:intergenic |
| ATTGGCTGAGAAGAATCAGC+GGG | 0.616730 | 4.4:-8056465 | MS.gene49704:CDS |
| AGGGTTCTGGTAGTTCTGGT+AGG | 0.617467 | 4.4:-8061456 | MS.gene49704:CDS |
| TCCGGCGGATCCAGGAAACA+TGG | 0.618063 | 4.4:+8061651 | None:intergenic |
| GCTTCTGGATGAATCCATTG+GGG | 0.620153 | 4.4:+8059485 | None:intergenic |
| CGTCGAAACTGGAGGAGTCG+CGG | 0.622735 | 4.4:+8061743 | None:intergenic |
| AACATGGAATCCAAATCGAA+GGG | 0.624907 | 4.4:+8061667 | None:intergenic |
| CCAGAGGTCTCAGGAGCGTG+TGG | 0.625131 | 4.4:-8056635 | MS.gene49704:intron |
| GATTTCGGTATCAAACCTCA+AGG | 0.625698 | 4.4:-8061901 | MS.gene49704:CDS |
| TCTTGAAGGCGGTGGAGCAG+TGG | 0.630091 | 4.4:+8058960 | None:intergenic |
| AACGGAATCGGATGTACGAG+CGG | 0.632703 | 4.4:+8061792 | None:intergenic |
| GGGAAAGAGCTACAGCTGCG+AGG | 0.632894 | 4.4:-8058198 | MS.gene49704:CDS |
| TTTGAAGATGATGATTTAGG+TGG | 0.635995 | 4.4:+8061856 | None:intergenic |
| GAAGAGATCGTCGAAACTGG+AGG | 0.639227 | 4.4:+8061735 | None:intergenic |
| CCAGATAAAGTACAGCAGAA+GGG | 0.639268 | 4.4:-8054547 | MS.gene49704:CDS |
| GGTTTATCATAAACCGGTGG+CGG | 0.641019 | 4.4:+8061619 | None:intergenic |
| CTTCAAGACCACCTCCTCCA+CGG | 0.642558 | 4.4:-8058945 | MS.gene49704:CDS |
| AGAGTTTCAAGAAGTTGAAG+GGG | 0.644368 | 4.4:-8056695 | MS.gene49704:CDS |
| CATTCCCATTGAAAGTGATG+GGG | 0.644400 | 4.4:-8059193 | MS.gene49704:CDS |
| GTGGAATAAATTCAATTCCG+AGG | 0.646105 | 4.4:-8053695 | MS.gene49704:CDS |
| AAGCACGCCAGAGAGCTGAG+AGG | 0.648062 | 4.4:-8058372 | MS.gene49704:CDS |
| GTAAGGATAACAGTGCTCCA+GGG | 0.648086 | 4.4:-8059302 | MS.gene49704:CDS |
| TGCGGCCATGAAGGAAGCAA+TGG | 0.656878 | 4.4:-8058668 | MS.gene49704:CDS |
| AAAGGTAGGCATGTAAAATG+GGG | 0.656986 | 4.4:+8059158 | None:intergenic |
| CTAGGTTGGAACGCCACCAG+AGG | 0.658505 | 4.4:-8056651 | MS.gene49704:CDS |
| GCAGATGCAACAAGGAAAAG+CGG | 0.658611 | 4.4:-8057654 | MS.gene49704:CDS |
| TAGGGAGAGAGAGCATACAA+AGG | 0.660083 | 4.4:-8058608 | MS.gene49704:CDS |
| TCGTTCACGAGCTTCTCGTG+TGG | 0.664835 | 4.4:+8058405 | None:intergenic |
| TGAATGATTATCAGGTGCTG+TGG | 0.671340 | 4.4:-8054663 | MS.gene49704:intron |
| CATTGGCTGAGAAGAATCAG+CGG | 0.682639 | 4.4:-8056466 | MS.gene49704:CDS |
| TGGCGGTGAAGAATTTCCGG+CGG | 0.690962 | 4.4:+8061636 | None:intergenic |
| AGAAAACTTGGAATCATGTG+AGG | 0.691683 | 4.4:-8059028 | MS.gene49704:CDS |
| AGATGACAGAGCTATACCAG+AGG | 0.698203 | 4.4:-8058551 | MS.gene49704:CDS |
| ACCACTATCCGATTTCGGCG+AGG | 0.700962 | 4.4:+8061519 | None:intergenic |
| GATGAACCAACAGAAGAATG+GGG | 0.701521 | 4.4:-8058089 | MS.gene49704:CDS |
| AAGGTAGGCATGTAAAATGG+GGG | 0.703036 | 4.4:+8059159 | None:intergenic |
| TGATGAGTTAGAGATGAACA+CGG | 0.706185 | 4.4:-8058695 | MS.gene49704:CDS |
| ACCGGTTTATCATAAACCGG+TGG | 0.707473 | 4.4:+8061616 | None:intergenic |
| TTTCTGGGATGAGTCCTGAG+AGG | 0.717258 | 4.4:-8059230 | MS.gene49704:CDS |
| AGGTGGTTGAGACATTGGAG+CGG | 0.727912 | 4.4:+8061873 | None:intergenic |
| AGGGTCATGAGAGAATCGAG+TGG | 0.729101 | 4.4:+8058834 | None:intergenic |
| AGTACATACATATTGCAAGG+TGG | 0.748823 | 4.4:+8056122 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 8053684 | 8061956 | 8053684 | ID=MS.gene49704 |
| chr4.4 | mRNA | 8053684 | 8061956 | 8053684 | ID=MS.gene49704.t1;Parent=MS.gene49704 |
| chr4.4 | exon | 8061367 | 8061956 | 8061367 | ID=MS.gene49704.t1.exon1;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8061367 | 8061956 | 8061367 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8058015 | 8059611 | 8058015 | ID=MS.gene49704.t1.exon2;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8058015 | 8059611 | 8058015 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8057586 | 8057679 | 8057586 | ID=MS.gene49704.t1.exon3;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8057586 | 8057679 | 8057586 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8056636 | 8056733 | 8056636 | ID=MS.gene49704.t1.exon4;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8056636 | 8056733 | 8056636 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8056430 | 8056495 | 8056430 | ID=MS.gene49704.t1.exon5;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8056430 | 8056495 | 8056430 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8056131 | 8056220 | 8056131 | ID=MS.gene49704.t1.exon6;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8056131 | 8056220 | 8056131 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8054495 | 8054671 | 8054495 | ID=MS.gene49704.t1.exon7;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8054495 | 8054671 | 8054495 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| chr4.4 | exon | 8053684 | 8053722 | 8053684 | ID=MS.gene49704.t1.exon8;Parent=MS.gene49704.t1 |
| chr4.4 | CDS | 8053684 | 8053722 | 8053684 | ID=cds.MS.gene49704.t1;Parent=MS.gene49704.t1 |
| Gene Sequence |
| Protein sequence |
