Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49841.t1 | KEH25549.1 | 96.8 | 63 | 2 | 0 | 1 | 63 | 81 | 143 | 1.10E-28 | 135.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49841.t1 | Q9FLD7 | 57.1 | 63 | 27 | 0 | 1 | 63 | 214 | 276 | 6.1e-17 | 87.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49841.t1 | A0A072U706 | 96.8 | 63 | 2 | 0 | 1 | 63 | 81 | 143 | 7.6e-29 | 135.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052863 | MS.gene49841 | 0.801745 | 7.79E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCAAATATCTGATTTCTTT+TGG | 0.221928 | 6.3:+19198677 | None:intergenic |
| GCCAAAAGAAATCAGATATT+TGG | 0.232766 | 6.3:-19198678 | MS.gene49841:CDS |
| CATTCACCATAGCAGTCATT+TGG | 0.315591 | 6.3:-19199216 | MS.gene49841:CDS |
| AGTGCTGGATCTATAAATAT+TGG | 0.340737 | 6.3:+19198706 | None:intergenic |
| ATTCACCATAGCAGTCATTT+GGG | 0.401509 | 6.3:-19199215 | MS.gene49841:CDS |
| TGAATGAAATTCATATGCTT+TGG | 0.436558 | 6.3:+19199233 | None:intergenic |
| ACCATAGCAGTCATTTGGGT+TGG | 0.447311 | 6.3:-19199211 | MS.gene49841:CDS |
| TATATAGTTTAAAAGCTGTT+GGG | 0.477917 | 6.3:+19198632 | None:intergenic |
| GTATATAGTTTAAAAGCTGT+TGG | 0.503019 | 6.3:+19198631 | None:intergenic |
| ATGCTTTGGAACTCCAGAAA+AGG | 0.544312 | 6.3:+19199247 | None:intergenic |
| AAAGAAATCAGATATTTGGC+AGG | 0.557557 | 6.3:-19198674 | MS.gene49841:CDS |
| TTGGAACTCCAGAAAAGGCA+TGG | 0.584028 | 6.3:+19199252 | None:intergenic |
| TCCAACCCAAATGACTGCTA+TGG | 0.598350 | 6.3:+19199210 | None:intergenic |
| TTTGGCAGGTAACTAAGCAA+AGG | 0.625832 | 6.3:-19198660 | MS.gene49841:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAAAATATTTAGTCTAT+TGG | - | chr6.3:19198865-19198884 | MS.gene49841:intron | 10.0% |
| !!! | AATTAATAAACTTTTAGTAT+AGG | + | chr6.3:19199041-19199060 | None:intergenic | 10.0% |
| !!! | ATTAATAAACTTTTAGTATA+GGG | + | chr6.3:19199040-19199059 | None:intergenic | 10.0% |
| !!! | AATTAGAATTTTGAGTAAAA+TGG | + | chr6.3:19199126-19199145 | None:intergenic | 15.0% |
| !!! | TTAATAAACTTTTAGTATAG+GGG | + | chr6.3:19199039-19199058 | None:intergenic | 15.0% |
| !! | TATATAGTTTAAAAGCTGTT+GGG | + | chr6.3:19199259-19199278 | None:intergenic | 20.0% |
| ! | CCAATTTAGTCACTAAAATT+TGG | + | chr6.3:19199081-19199100 | None:intergenic | 25.0% |
| ! | GTATATAGTTTAAAAGCTGT+TGG | + | chr6.3:19199260-19199279 | None:intergenic | 25.0% |
| ! | TGAATGAAATTCATATGCTT+TGG | + | chr6.3:19198658-19198677 | None:intergenic | 25.0% |
| !! | ATATTTTCCAGCAATAAAAG+AGG | - | chr6.3:19199155-19199174 | MS.gene49841:intron | 25.0% |
| !! | TATTTTCCAGCAATAAAAGA+GGG | - | chr6.3:19199156-19199175 | MS.gene49841:intron | 25.0% |
| !!! | CCAAATTTTAGTGACTAAAT+TGG | - | chr6.3:19199078-19199097 | MS.gene49841:intron | 25.0% |
| AAAGAAATCAGATATTTGGC+AGG | - | chr6.3:19199214-19199233 | MS.gene49841:CDS | 30.0% | |
| GCCAAAAGAAATCAGATATT+TGG | - | chr6.3:19199210-19199229 | MS.gene49841:CDS | 30.0% | |
| ! | AGTGCTGGATCTATAAATAT+TGG | + | chr6.3:19199185-19199204 | None:intergenic | 30.0% |
| ! | GCCAAATATCTGATTTCTTT+TGG | + | chr6.3:19199214-19199233 | None:intergenic | 30.0% |
| !! | AGTCTCTAAAATTACCTCAA+AGG | - | chr6.3:19198902-19198921 | MS.gene49841:intron | 30.0% |
| ATTCACCATAGCAGTCATTT+GGG | - | chr6.3:19198673-19198692 | MS.gene49841:CDS | 35.0% | |
| TCATCACATTAGTTCCTTTG+AGG | + | chr6.3:19198919-19198938 | None:intergenic | 35.0% | |
| !! | TTCTTTTGGCTATAAAGTGC+TGG | + | chr6.3:19199200-19199219 | None:intergenic | 35.0% |
| CATTCACCATAGCAGTCATT+TGG | - | chr6.3:19198672-19198691 | MS.gene49841:CDS | 40.0% | |
| TTTGGCAGGTAACTAAGCAA+AGG | - | chr6.3:19199228-19199247 | MS.gene49841:CDS | 40.0% | |
| !! | ATGCTTTGGAACTCCAGAAA+AGG | + | chr6.3:19198644-19198663 | None:intergenic | 40.0% |
| ACCATAGCAGTCATTTGGGT+TGG | - | chr6.3:19198677-19198696 | MS.gene49841:CDS | 45.0% | |
| TCCAACCCAAATGACTGCTA+TGG | + | chr6.3:19198681-19198700 | None:intergenic | 45.0% | |
| TTGGAACTCCAGAAAAGGCA+TGG | + | chr6.3:19198639-19198658 | None:intergenic | 45.0% | |
| ! | AGGGGCATAGTACAAGTGAT+TGG | + | chr6.3:19199021-19199040 | None:intergenic | 45.0% |
| ! | TGGCTTCCCTCTTTTATTGC+TGG | + | chr6.3:19199165-19199184 | None:intergenic | 45.0% |
| !!! | AATTAAATTCTATAATTTTA+GGG | + | chr6.3:19198812-19198831 | None:intergenic | 5.0% |
| !!! | TAATTAAATTCTATAATTTT+AGG | + | chr6.3:19198813-19198832 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 19198629 | 19199281 | 19198629 | ID=MS.gene49841 |
| chr6.3 | mRNA | 19198629 | 19199281 | 19198629 | ID=MS.gene49841.t1;Parent=MS.gene49841 |
| chr6.3 | exon | 19199206 | 19199281 | 19199206 | ID=MS.gene49841.t1.exon1;Parent=MS.gene49841.t1 |
| chr6.3 | CDS | 19199206 | 19199281 | 19199206 | ID=cds.MS.gene49841.t1;Parent=MS.gene49841.t1 |
| chr6.3 | exon | 19198629 | 19198744 | 19198629 | ID=MS.gene49841.t1.exon2;Parent=MS.gene49841.t1 |
| chr6.3 | CDS | 19198629 | 19198744 | 19198629 | ID=cds.MS.gene49841.t1;Parent=MS.gene49841.t1 |
| Gene Sequence |
| Protein sequence |
