Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50151.t1 | XP_003616435.1 | 97.8 | 90 | 1 | 1 | 1 | 90 | 1 | 89 | 7.20E-39 | 169.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50151.t1 | G7KGR6 | 97.8 | 90 | 1 | 1 | 1 | 90 | 1 | 89 | 5.2e-39 | 169.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055339 | MS.gene50151 | 0.805678 | 1.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50151.t1 | MTR_5g080260 | 97.778 | 90 | 1 | 1 | 1 | 90 | 1 | 89 | 2.84e-59 | 176 |
| MS.gene50151.t1 | MTR_3g067980 | 72.727 | 77 | 17 | 3 | 16 | 90 | 19 | 93 | 2.53e-30 | 103 |
| MS.gene50151.t1 | MTR_4g064520 | 37.255 | 102 | 32 | 2 | 20 | 89 | 1 | 102 | 3.09e-15 | 65.1 |
| MS.gene50151.t1 | MTR_2g097570 | 61.111 | 36 | 14 | 0 | 55 | 90 | 51 | 86 | 9.87e-13 | 58.5 |
| MS.gene50151.t1 | MTR_8g023610 | 57.895 | 38 | 16 | 0 | 47 | 84 | 15 | 52 | 4.54e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50151.t1 | AT2G39705 | 62.667 | 75 | 26 | 2 | 18 | 90 | 13 | 87 | 5.74e-26 | 92.0 |
| MS.gene50151.t1 | AT3G55515 | 51.471 | 68 | 31 | 1 | 23 | 90 | 9 | 74 | 1.41e-18 | 73.2 |
| MS.gene50151.t1 | AT2G36985 | 61.290 | 31 | 12 | 0 | 56 | 86 | 15 | 45 | 1.37e-11 | 54.7 |
| MS.gene50151.t1 | AT3G14362 | 65.714 | 35 | 12 | 0 | 55 | 89 | 13 | 47 | 3.56e-11 | 53.5 |
| MS.gene50151.t1 | AT4G13395 | 47.059 | 51 | 24 | 1 | 43 | 90 | 4 | 54 | 8.45e-11 | 52.8 |
| MS.gene50151.t1 | AT1G53708 | 68.571 | 35 | 11 | 0 | 55 | 89 | 13 | 47 | 9.87e-11 | 53.9 |
Find 11 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGCTTCTTCTTCTTCTA+TGG | 0.160103 | 5.4:+62994554 | None:intergenic |
| TAGAGAGGAGGTTGAGGTTG+AGG | 0.358701 | 5.4:-62994588 | None:intergenic |
| AGAGAGGAGGTTGAGGTTGA+GGG | 0.382443 | 5.4:-62994587 | None:intergenic |
| GTGTTACAATGCTCATTTGC+TGG | 0.423945 | 5.4:+62994784 | MS.gene50151:CDS |
| TGGAAGCTATCAAAGAAAGA+AGG | 0.500507 | 5.4:+62994624 | MS.gene50151:CDS |
| TCAAAGTAGAGAGGAGGTTG+AGG | 0.504111 | 5.4:-62994594 | None:intergenic |
| GAGAGGAGGTTGAGGTTGAG+GGG | 0.511193 | 5.4:-62994586 | None:intergenic |
| CATAGAAGAAGAAGAAGCCA+TGG | 0.520025 | 5.4:-62994553 | None:intergenic |
| GGAAGCTATCAAAGAAAGAA+GGG | 0.569563 | 5.4:+62994625 | MS.gene50151:CDS |
| CACGTTTCTACATCATGAGG+CGG | 0.662634 | 5.4:+62994760 | MS.gene50151:CDS |
| GAGCACGTTTCTACATCATG+AGG | 0.665259 | 5.4:+62994757 | MS.gene50151:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTCTACTTTGATGAAAAA+TGG | + | chr5.4:62994604-62994623 | MS.gene50151:CDS | 30.0% | |
| ! | CATTTTTCATCAAAGTAGAG+AGG | - | chr5.4:62994606-62994625 | None:intergenic | 30.0% |
| GGAAGCTATCAAAGAAAGAA+GGG | + | chr5.4:62994625-62994644 | MS.gene50151:CDS | 35.0% | |
| TGGAAGCTATCAAAGAAAGA+AGG | + | chr5.4:62994624-62994643 | MS.gene50151:CDS | 35.0% | |
| ! | TTTTCATCAAAGTAGAGAGG+AGG | - | chr5.4:62994603-62994622 | None:intergenic | 35.0% |
| GTGTTACAATGCTCATTTGC+TGG | + | chr5.4:62994784-62994803 | MS.gene50151:CDS | 40.0% | |
| CACGTTTCTACATCATGAGG+CGG | + | chr5.4:62994760-62994779 | MS.gene50151:CDS | 45.0% | |
| GAGCACGTTTCTACATCATG+AGG | + | chr5.4:62994757-62994776 | MS.gene50151:CDS | 45.0% | |
| TCAAAGTAGAGAGGAGGTTG+AGG | - | chr5.4:62994597-62994616 | None:intergenic | 45.0% | |
| AGAGAGGAGGTTGAGGTTGA+GGG | - | chr5.4:62994590-62994609 | None:intergenic | 50.0% | |
| TAGAGAGGAGGTTGAGGTTG+AGG | - | chr5.4:62994591-62994610 | None:intergenic | 50.0% | |
| GAGAGGAGGTTGAGGTTGAG+GGG | - | chr5.4:62994589-62994608 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 62994555 | 62994827 | 62994555 | ID=MS.gene50151 |
| chr5.4 | mRNA | 62994555 | 62994827 | 62994555 | ID=MS.gene50151.t1;Parent=MS.gene50151 |
| chr5.4 | exon | 62994555 | 62994827 | 62994555 | ID=MS.gene50151.t1.exon1;Parent=MS.gene50151.t1 |
| chr5.4 | CDS | 62994555 | 62994827 | 62994555 | ID=cds.MS.gene50151.t1;Parent=MS.gene50151.t1 |
| Gene Sequence |
| Protein sequence |
