Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50172.t1 | RHN56971.1 | 100 | 35 | 0 | 0 | 52 | 86 | 51 | 85 | 4.20E-12 | 80.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50172.t1 | Q8L3X8 | 94.3 | 35 | 2 | 0 | 52 | 86 | 51 | 85 | 8.6e-14 | 77.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50172.t1 | G7KBR1 | 100.0 | 35 | 0 | 0 | 52 | 86 | 51 | 85 | 3.0e-12 | 80.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050384 | MS.gene50172 | 0.800697 | 1.28E-48 | -1.69E-46 |
| MS.gene051328 | MS.gene50172 | 0.814071 | 1.86E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50172.t1 | MTR_5g079870 | 96.552 | 87 | 0 | 2 | 1 | 86 | 1 | 85 | 3.63e-47 | 150 |
| MS.gene50172.t1 | MTR_5g079870 | 96.552 | 87 | 0 | 2 | 1 | 86 | 1 | 85 | 4.66e-47 | 151 |
| MS.gene50172.t1 | MTR_4g118630 | 72.973 | 37 | 10 | 0 | 50 | 86 | 50 | 86 | 1.85e-14 | 66.6 |
| MS.gene50172.t1 | MTR_1g054675 | 66.667 | 39 | 13 | 0 | 48 | 86 | 43 | 81 | 4.46e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50172.t1 | AT3G55460 | 76.744 | 86 | 19 | 1 | 1 | 86 | 1 | 85 | 4.34e-20 | 81.3 |
| MS.gene50172.t1 | AT3G13570 | 55.556 | 63 | 28 | 0 | 24 | 86 | 13 | 75 | 1.89e-15 | 68.9 |
| MS.gene50172.t1 | AT1G55310 | 55.556 | 63 | 27 | 1 | 24 | 86 | 13 | 74 | 2.88e-14 | 65.1 |
| MS.gene50172.t1 | AT1G55310 | 55.556 | 63 | 27 | 1 | 24 | 86 | 13 | 74 | 2.88e-14 | 65.1 |
| MS.gene50172.t1 | AT1G55310 | 55.556 | 63 | 27 | 1 | 24 | 86 | 13 | 74 | 5.47e-14 | 65.5 |
| MS.gene50172.t1 | AT1G55310 | 55.556 | 63 | 27 | 1 | 24 | 86 | 13 | 74 | 5.50e-14 | 65.1 |
| MS.gene50172.t1 | AT5G18810 | 62.162 | 37 | 14 | 0 | 50 | 86 | 49 | 85 | 1.07e-12 | 61.2 |
| MS.gene50172.t1 | AT5G18810 | 62.162 | 37 | 14 | 0 | 50 | 86 | 49 | 85 | 1.08e-12 | 61.2 |
Find 47 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGGTGGTGGTGGAGGTGG+TGG | 0.246287 | 5.4:+62713752 | MS.gene50172:CDS |
| GACTCCCATTGTTGCTTTCC+CGG | 0.253796 | 5.4:-62713823 | None:intergenic |
| TACGGTGGTGGTGGTGGAGG+TGG | 0.291679 | 5.4:+62713785 | MS.gene50172:CDS |
| GGTGGAAGGGGTGGTGGTGG+AGG | 0.293749 | 5.4:+62713746 | MS.gene50172:CDS |
| GGTGGTGGTGGAGGTGGAGG+TGG | 0.295066 | 5.4:+62713791 | MS.gene50172:CDS |
| GGTGGTGGAGGTGGAGGTGG+AGG | 0.304108 | 5.4:+62713794 | MS.gene50172:CDS |
| GGAAGGGGTGGTGGTGGAGG+TGG | 0.318067 | 5.4:+62713749 | MS.gene50172:CDS |
| GGTGGTGGTGGTGGAGGTGG+AGG | 0.326296 | 5.4:+62713788 | MS.gene50172:CDS |
| TTTGGTGAGGAACATTCCTT+TGG | 0.338345 | 5.4:+62713847 | MS.gene50172:CDS |
| TACGGTGGAAGGGGTGGTGG+TGG | 0.340275 | 5.4:+62713743 | MS.gene50172:CDS |
| GTGGTGGTGGAGGTGGTGGC+AGG | 0.354325 | 5.4:+62713756 | MS.gene50172:CDS |
| AAGCAACAATGGGAGTCTTT+TGG | 0.361759 | 5.4:+62713829 | MS.gene50172:CDS |
| GGATACGGTGGAAGGGGTGG+TGG | 0.373851 | 5.4:+62713740 | MS.gene50172:CDS |
| AGAGCGCCGTTCGAGCGATT+TGG | 0.380468 | 5.4:+62713890 | MS.gene50172:CDS |
| GGATACGGTGGTGGTGGTGG+AGG | 0.422326 | 5.4:+62713782 | MS.gene50172:CDS |
| AGAGGATACGGTGGTGGTGG+TGG | 0.426776 | 5.4:+62713779 | MS.gene50172:CDS |
| AACGGCGCTCTCAGCTCCTC+AGG | 0.453718 | 5.4:-62713878 | None:intergenic |
| ATACCGAAGGATTATTACTC+TGG | 0.454877 | 5.4:+62713932 | MS.gene50172:CDS |
| AGAGGATACGGTGGAAGGGG+TGG | 0.456533 | 5.4:+62713737 | MS.gene50172:CDS |
| AGGAGAGGATACGGTGGTGG+TGG | 0.464641 | 5.4:+62713776 | MS.gene50172:CDS |
| GGTGGAGGTGGTGGCAGGAG+AGG | 0.467395 | 5.4:+62713761 | MS.gene50172:CDS |
| GGTGGTGGCAGGAGAGGATA+CGG | 0.477335 | 5.4:+62713767 | MS.gene50172:CDS |
| CGGAGAGGATACGGTGGAAG+GGG | 0.485150 | 5.4:+62713734 | MS.gene50172:CDS |
| GCCGTTCGAGCGATTTGGAC+CGG | 0.493695 | 5.4:+62713895 | MS.gene50172:CDS |
| CACCCAGAGTAATAATCCTT+CGG | 0.507340 | 5.4:-62713935 | None:intergenic |
| GGCAGGAGAGGATACGGTGG+TGG | 0.517250 | 5.4:+62713773 | MS.gene50172:CDS |
| CGTGGAGGACTGTAGTAACC+TGG | 0.521171 | 5.4:-62713713 | None:intergenic |
| GTGGAGGTGGAGGTAGACGC+CGG | 0.524197 | 5.4:+62713804 | MS.gene50172:CDS |
| ACGGAGAGGATACGGTGGAA+GGG | 0.525215 | 5.4:+62713733 | MS.gene50172:CDS |
| CACGGAGAGGATACGGTGGA+AGG | 0.525743 | 5.4:+62713732 | MS.gene50172:CDS |
| AGTCCTCCACGGAGAGGATA+CGG | 0.532850 | 5.4:+62713725 | MS.gene50172:CDS |
| TCCTTTGGATTGTAGACCTG+AGG | 0.559671 | 5.4:+62713862 | MS.gene50172:CDS |
| TCGTGATGTTTACATACCGA+AGG | 0.573384 | 5.4:+62713919 | MS.gene50172:CDS |
| TGGAGGTGGAGGTAGACGCC+GGG | 0.573511 | 5.4:+62713805 | MS.gene50172:CDS |
| GGTATGTAAACATCACGAAC+CGG | 0.574540 | 5.4:-62713914 | None:intergenic |
| ACCGGTCCAAATCGCTCGAA+CGG | 0.577721 | 5.4:-62713896 | None:intergenic |
| TACCGAAGGATTATTACTCT+GGG | 0.581860 | 5.4:+62713933 | MS.gene50172:CDS |
| GACGCCGGGAAAGCAACAAT+GGG | 0.582698 | 5.4:+62713819 | MS.gene50172:CDS |
| AGACGCCGGGAAAGCAACAA+TGG | 0.585883 | 5.4:+62713818 | MS.gene50172:CDS |
| TCCTCAGGTCTACAATCCAA+AGG | 0.618913 | 5.4:-62713863 | None:intergenic |
| AAAATGCGGAGGAACAGTCC+AGG | 0.636769 | 5.4:+62713695 | None:intergenic |
| CCACCGTATCCTCTCCGTGG+AGG | 0.661599 | 5.4:-62713728 | None:intergenic |
| CAGGTTACTACAGTCCTCCA+CGG | 0.673504 | 5.4:+62713714 | MS.gene50172:CDS |
| CTTCCACCGTATCCTCTCCG+TGG | 0.674969 | 5.4:-62713731 | None:intergenic |
| TACTACAGTCCTCCACGGAG+AGG | 0.678746 | 5.4:+62713719 | MS.gene50172:CDS |
| CCTCCACGGAGAGGATACGG+TGG | 0.734592 | 5.4:+62713728 | MS.gene50172:CDS |
| GGTGGCAGGAGAGGATACGG+TGG | 0.740224 | 5.4:+62713770 | MS.gene50172:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACCGAAGGATTATTACTC+TGG | + | chr5.4:62713932-62713951 | MS.gene50172:CDS | 35.0% | |
| TACCGAAGGATTATTACTCT+GGG | + | chr5.4:62713933-62713952 | MS.gene50172:CDS | 35.0% | |
| CACCCAGAGTAATAATCCTT+CGG | - | chr5.4:62713938-62713957 | None:intergenic | 40.0% | |
| GGTATGTAAACATCACGAAC+CGG | - | chr5.4:62713917-62713936 | None:intergenic | 40.0% | |
| TCGTGATGTTTACATACCGA+AGG | + | chr5.4:62713919-62713938 | MS.gene50172:CDS | 40.0% | |
| TTTGGTGAGGAACATTCCTT+TGG | + | chr5.4:62713847-62713866 | MS.gene50172:CDS | 40.0% | |
| ! | AAGCAACAATGGGAGTCTTT+TGG | + | chr5.4:62713829-62713848 | MS.gene50172:CDS | 40.0% |
| TCCTCAGGTCTACAATCCAA+AGG | - | chr5.4:62713866-62713885 | None:intergenic | 45.0% | |
| TCCTTTGGATTGTAGACCTG+AGG | + | chr5.4:62713862-62713881 | MS.gene50172:CDS | 45.0% | |
| ! | ACAATGGGAGTCTTTTGGTG+AGG | + | chr5.4:62713834-62713853 | MS.gene50172:CDS | 45.0% |
| CAGGTTACTACAGTCCTCCA+CGG | + | chr5.4:62713714-62713733 | MS.gene50172:CDS | 50.0% | |
| !! | GACTCCCATTGTTGCTTTCC+CGG | - | chr5.4:62713826-62713845 | None:intergenic | 50.0% |
| ACCGGTCCAAATCGCTCGAA+CGG | - | chr5.4:62713899-62713918 | None:intergenic | 55.0% | |
| ACGGAGAGGATACGGTGGAA+GGG | + | chr5.4:62713733-62713752 | MS.gene50172:CDS | 55.0% | |
| AGACGCCGGGAAAGCAACAA+TGG | + | chr5.4:62713818-62713837 | MS.gene50172:CDS | 55.0% | |
| AGTCCTCCACGGAGAGGATA+CGG | + | chr5.4:62713725-62713744 | MS.gene50172:CDS | 55.0% | |
| CGTGGAGGACTGTAGTAACC+TGG | - | chr5.4:62713716-62713735 | None:intergenic | 55.0% | |
| GACGCCGGGAAAGCAACAAT+GGG | + | chr5.4:62713819-62713838 | MS.gene50172:CDS | 55.0% | |
| TACTACAGTCCTCCACGGAG+AGG | + | chr5.4:62713719-62713738 | MS.gene50172:CDS | 55.0% | |
| AGAGGATACGGTGGAAGGGG+TGG | + | chr5.4:62713737-62713756 | MS.gene50172:CDS | 60.0% | |
| CACGGAGAGGATACGGTGGA+AGG | + | chr5.4:62713732-62713751 | MS.gene50172:CDS | 60.0% | |
| CGGAGAGGATACGGTGGAAG+GGG | + | chr5.4:62713734-62713753 | MS.gene50172:CDS | 60.0% | |
| CTTCCACCGTATCCTCTCCG+TGG | - | chr5.4:62713734-62713753 | None:intergenic | 60.0% | |
| ! | AGGAGAGGATACGGTGGTGG+TGG | + | chr5.4:62713776-62713795 | MS.gene50172:CDS | 60.0% |
| ! | GCCGTTCGAGCGATTTGGAC+CGG | + | chr5.4:62713895-62713914 | MS.gene50172:CDS | 60.0% |
| ! | GGTGGTGGCAGGAGAGGATA+CGG | + | chr5.4:62713767-62713786 | MS.gene50172:CDS | 60.0% |
| !! | AGAGCGCCGTTCGAGCGATT+TGG | + | chr5.4:62713890-62713909 | MS.gene50172:CDS | 60.0% |
| !! | AGAGGATACGGTGGTGGTGG+TGG | + | chr5.4:62713779-62713798 | MS.gene50172:CDS | 60.0% |
| AACGGCGCTCTCAGCTCCTC+AGG | - | chr5.4:62713881-62713900 | None:intergenic | 65.0% | |
| CCACCGTATCCTCTCCGTGG+AGG | - | chr5.4:62713731-62713750 | None:intergenic | 65.0% | |
| CCTCCACGGAGAGGATACGG+TGG | + | chr5.4:62713728-62713747 | MS.gene50172:CDS | 65.0% | |
| GGATACGGTGGAAGGGGTGG+TGG | + | chr5.4:62713740-62713759 | MS.gene50172:CDS | 65.0% | |
| GGCAGGAGAGGATACGGTGG+TGG | + | chr5.4:62713773-62713792 | MS.gene50172:CDS | 65.0% | |
| GGTGGCAGGAGAGGATACGG+TGG | + | chr5.4:62713770-62713789 | MS.gene50172:CDS | 65.0% | |
| GTGGAGGTGGAGGTAGACGC+CGG | + | chr5.4:62713804-62713823 | MS.gene50172:CDS | 65.0% | |
| TGGAGGTGGAGGTAGACGCC+GGG | + | chr5.4:62713805-62713824 | MS.gene50172:CDS | 65.0% | |
| ! | TACGGTGGAAGGGGTGGTGG+TGG | + | chr5.4:62713743-62713762 | MS.gene50172:CDS | 65.0% |
| !! | GGATACGGTGGTGGTGGTGG+AGG | + | chr5.4:62713782-62713801 | MS.gene50172:CDS | 65.0% |
| !! | TACGGTGGTGGTGGTGGAGG+TGG | + | chr5.4:62713785-62713804 | MS.gene50172:CDS | 65.0% |
| ! | GGTGGAGGTGGTGGCAGGAG+AGG | + | chr5.4:62713761-62713780 | MS.gene50172:CDS | 70.0% |
| !! | AGGGGTGGTGGTGGAGGTGG+TGG | + | chr5.4:62713752-62713771 | MS.gene50172:CDS | 70.0% |
| !! | GGAAGGGGTGGTGGTGGAGG+TGG | + | chr5.4:62713749-62713768 | MS.gene50172:CDS | 70.0% |
| !! | GGTGGAAGGGGTGGTGGTGG+AGG | + | chr5.4:62713746-62713765 | MS.gene50172:CDS | 70.0% |
| !! | GGTGGTGGAGGTGGAGGTGG+AGG | + | chr5.4:62713794-62713813 | MS.gene50172:CDS | 70.0% |
| !! | GGTGGTGGTGGAGGTGGAGG+TGG | + | chr5.4:62713791-62713810 | MS.gene50172:CDS | 70.0% |
| !! | GGTGGTGGTGGTGGAGGTGG+AGG | + | chr5.4:62713788-62713807 | MS.gene50172:CDS | 70.0% |
| !! | GTGGTGGTGGAGGTGGTGGC+AGG | + | chr5.4:62713756-62713775 | MS.gene50172:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 62713698 | 62713958 | 62713698 | ID=MS.gene50172 |
| chr5.4 | mRNA | 62713698 | 62713958 | 62713698 | ID=MS.gene50172.t1;Parent=MS.gene50172 |
| chr5.4 | exon | 62713698 | 62713958 | 62713698 | ID=MS.gene50172.t1.exon1;Parent=MS.gene50172.t1 |
| chr5.4 | CDS | 62713698 | 62713958 | 62713698 | ID=cds.MS.gene50172.t1;Parent=MS.gene50172.t1 |
| Gene Sequence |
| Protein sequence |
