AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50212

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50212.t1 MTR_5g072570 95.532 470 21 0 1 470 1 470 0.0 945
MS.gene50212.t1 MTR_3g090130 78.205 468 97 2 1 468 1 463 0.0 770
MS.gene50212.t1 MTR_3g090150 75.738 474 105 4 1 468 1 470 0.0 748
MS.gene50212.t1 MTR_7g021130 48.157 434 216 6 30 461 31 457 1.97e-143 419
MS.gene50212.t1 MTR_8g099930 46.804 438 226 5 28 463 32 464 5.04e-141 413
MS.gene50212.t1 MTR_3g498755 46.136 440 224 7 28 461 13 445 9.73e-140 409
MS.gene50212.t1 MTR_3g434850 46.725 458 226 7 9 461 10 454 3.52e-139 408
MS.gene50212.t1 MTR_2g449850 45.642 436 224 6 31 463 39 464 6.24e-130 384
MS.gene50212.t1 MTR_7g013790 42.437 476 243 9 6 465 8 468 2.22e-124 370
MS.gene50212.t1 MTR_3g034290 44.420 448 225 8 22 465 67 494 2.93e-123 369
MS.gene50212.t1 MTR_4g089155 43.043 460 232 11 19 463 43 487 4.86e-121 362
MS.gene50212.t1 MTR_4g075150 42.612 467 244 11 7 461 9 463 6.56e-120 359
MS.gene50212.t1 MTR_4g075150 42.888 464 245 11 7 461 9 461 7.09e-120 358
MS.gene50212.t1 MTR_3g079570 45.558 439 213 10 26 461 75 490 2.05e-119 358
MS.gene50212.t1 MTR_2g012860 43.274 446 231 7 28 462 40 474 2.59e-119 358
MS.gene50212.t1 MTR_0003s0560 44.444 441 225 7 22 461 66 487 6.48e-119 357
MS.gene50212.t1 MTR_3g079590 45.392 434 214 9 26 457 74 486 2.32e-118 356
MS.gene50212.t1 MTR_3g079560 44.270 445 223 9 22 463 70 492 1.04e-116 352
MS.gene50212.t1 MTR_2g012850 42.188 448 235 8 27 463 33 467 4.48e-116 349
MS.gene50212.t1 MTR_1g040370 41.136 440 202 6 28 462 29 416 9.70e-116 346
MS.gene50212.t1 MTR_3g079600 44.667 450 224 12 22 467 68 496 4.86e-115 347
MS.gene50212.t1 MTR_1g095560 40.299 469 260 9 4 464 3 459 4.05e-112 338
MS.gene50212.t1 MTR_3g079620 42.291 454 225 12 26 467 74 502 4.51e-112 340
MS.gene50212.t1 MTR_1g021010 43.936 437 216 11 30 462 76 487 6.00e-111 337
MS.gene50212.t1 MTR_3g079630 42.391 460 225 12 22 467 70 503 5.42e-110 335
MS.gene50212.t1 MTR_2g081620 38.675 468 267 9 9 468 15 470 8.20e-110 333
MS.gene50212.t1 MTR_1g020090 43.537 441 213 11 27 461 87 497 1.83e-109 333
MS.gene50212.t1 MTR_4g122950 38.557 485 253 12 9 464 12 480 1.78e-108 330
MS.gene50212.t1 MTR_2g063000 37.740 469 271 10 2 461 7 463 2.47e-108 329
MS.gene50212.t1 MTR_2g062990 40.509 432 242 9 33 461 43 462 5.85e-108 328
MS.gene50212.t1 MTR_1g021020 43.836 438 218 10 28 462 76 488 2.28e-107 327
MS.gene50212.t1 MTR_1g095540 40.590 441 246 8 27 463 30 458 9.22e-106 322
MS.gene50212.t1 MTR_1g095560 37.725 501 260 10 4 464 3 491 2.32e-105 322
MS.gene50212.t1 MTR_4g122960 37.759 482 259 13 9 464 12 478 1.14e-103 318
MS.gene50212.t1 MTR_2g062980 39.723 433 244 9 33 461 43 462 2.38e-103 317
MS.gene50212.t1 MTR_3g034290 44.134 358 180 7 22 375 67 408 4.88e-96 296
MS.gene50212.t1 MTR_1g102130 38.341 446 229 11 28 466 90 496 5.36e-94 293
MS.gene50212.t1 MTR_2g063000 33.691 466 263 11 2 461 7 432 1.50e-87 275
MS.gene50212.t1 MTR_0003s0560 48.872 266 127 4 22 286 66 323 2.91e-81 256
MS.gene50212.t1 MTR_7g051430 32.016 506 281 13 1 457 1 492 3.99e-77 249
MS.gene50212.t1 MTR_8g011350 31.791 497 276 13 9 457 11 492 9.37e-72 235
MS.gene50212.t1 MTR_7g080180 32.150 507 280 17 6 460 4 498 1.84e-71 234
MS.gene50212.t1 MTR_8g011350 31.290 473 262 13 9 433 11 468 7.46e-64 214
MS.gene50212.t1 MTR_0075s0010 34.271 391 181 11 26 409 4 325 1.44e-59 198
MS.gene50212.t1 MTR_7g080180 31.613 465 254 17 6 418 4 456 2.14e-59 202
MS.gene50212.t1 MTR_7g051430 37.884 293 168 7 1 287 1 285 1.10e-55 191
MS.gene50212.t1 MTR_2g018630 29.867 452 275 15 29 460 36 465 2.63e-52 183
MS.gene50212.t1 MTR_2g018600 28.160 451 291 14 29 460 33 469 7.89e-50 177
MS.gene50212.t1 MTR_4g021510 29.228 479 279 19 6 456 19 465 2.99e-48 172
MS.gene50212.t1 MTR_2g018590 27.789 475 306 17 6 460 12 469 3.56e-48 172
MS.gene50212.t1 MTR_4g021535 27.579 475 300 17 2 456 12 462 1.41e-46 168
MS.gene50212.t1 MTR_4g021520 27.578 446 285 15 30 456 40 466 4.22e-46 167
MS.gene50212.t1 MTR_4g021490 27.314 443 290 15 30 456 40 466 6.17e-46 166
MS.gene50212.t1 MTR_1g050545 30.324 432 254 13 41 457 81 480 1.62e-45 166
MS.gene50212.t1 MTR_4g021530 26.622 447 288 16 30 456 40 466 1.94e-44 162
MS.gene50212.t1 MTR_4g057585 27.563 439 269 12 35 457 87 492 4.37e-44 162
MS.gene50212.t1 MTR_4g057595 27.679 448 271 13 37 468 90 500 1.24e-43 160
MS.gene50212.t1 MTR_2g095750 27.934 426 280 12 40 460 97 500 3.02e-43 160
MS.gene50212.t1 MTR_4g021500 27.703 444 283 18 30 454 40 464 1.02e-42 162
MS.gene50212.t1 MTR_4g021500 26.424 439 286 16 36 456 482 901 1.29e-37 147
MS.gene50212.t1 MTR_4g021500 27.703 444 283 18 30 454 40 464 1.04e-42 162
MS.gene50212.t1 MTR_4g021500 26.340 429 281 15 36 456 482 883 3.68e-36 142
MS.gene50212.t1 MTR_3g100420 27.826 460 290 18 28 460 52 496 2.38e-42 157
MS.gene50212.t1 MTR_2g095730 27.126 435 271 13 40 458 91 495 9.83e-41 152
MS.gene50212.t1 MTR_1g094155 27.546 432 267 12 40 457 87 486 1.46e-40 152
MS.gene50212.t1 MTR_4g057570 27.635 427 277 13 41 458 87 490 8.79e-39 147
MS.gene50212.t1 MTR_8g007520 91.549 71 6 0 346 416 111 181 1.69e-38 138
MS.gene50212.t1 MTR_4g057600 27.626 438 259 13 41 458 93 492 3.68e-37 142
MS.gene50212.t1 MTR_3g045770 25.342 438 300 12 29 454 26 448 3.70e-37 142
MS.gene50212.t1 MTR_4g021520 27.206 408 259 15 30 418 40 428 9.60e-36 137
MS.gene50212.t1 MTR_2g018630 28.177 362 225 12 113 460 20 360 5.99e-35 134
MS.gene50212.t1 MTR_2g009200 26.049 453 268 16 28 456 26 435 2.15e-32 128
MS.gene50212.t1 MTR_5g097180 26.066 422 269 12 45 455 43 432 2.30e-32 128
MS.gene50212.t1 MTR_4g021535 25.684 475 262 19 2 456 12 415 2.58e-32 127
MS.gene50212.t1 MTR_4g084710 25.114 438 269 15 37 455 37 434 2.68e-31 125
MS.gene50212.t1 MTR_2g095710 26.018 442 269 16 34 460 85 483 1.27e-26 112
MS.gene50212.t1 MTR_1g051175 54.118 85 36 2 37 120 20 102 1.50e-21 94.7
MS.gene50212.t1 MTR_7g100560 27.962 211 86 3 36 246 65 209 1.47e-19 89.7
MS.gene50212.t1 MTR_4g056520 22.174 460 276 17 42 456 32 454 1.03e-17 85.9
MS.gene50212.t1 MTR_6g086380 74.419 43 11 0 422 464 58 100 3.82e-14 68.6
MS.gene50212.t1 MTR_2g102960 82.857 35 6 0 346 380 36 70 1.31e-11 63.2
MS.gene50212.t1 MTR_2g084530 32.105 190 108 6 46 230 36 209 1.97e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50212.t1 AT5G42240 79.111 450 94 0 20 469 23 472 0.0 773
MS.gene50212.t1 AT1G43780 69.620 474 137 3 2 468 4 477 0.0 717
MS.gene50212.t1 AT5G42230 71.460 452 129 0 13 464 12 463 0.0 698
MS.gene50212.t1 AT5G42230 71.460 452 129 0 13 464 12 463 0.0 698
MS.gene50212.t1 AT2G12480 71.930 456 120 3 7 462 11 458 0.0 697
MS.gene50212.t1 AT2G12480 68.202 456 113 4 7 462 11 434 0.0 647
MS.gene50212.t1 AT2G12480 67.763 456 113 4 7 462 11 432 0.0 642
MS.gene50212.t1 AT2G12480 67.615 457 113 5 7 462 11 433 0.0 637
MS.gene50212.t1 AT2G33530 49.087 438 214 6 28 462 32 463 5.33e-149 433
MS.gene50212.t1 AT1G28110 48.292 439 214 7 28 462 30 459 2.22e-143 419
MS.gene50212.t1 AT1G28110 48.292 439 214 7 28 462 30 459 2.22e-143 419
MS.gene50212.t1 AT3G02110 42.857 483 242 10 1 462 3 472 1.11e-121 363
MS.gene50212.t1 AT3G63470 43.596 445 233 9 22 465 74 501 2.58e-120 361
MS.gene50212.t1 AT2G24010 43.213 442 230 8 27 463 28 453 1.99e-119 357
MS.gene50212.t1 AT4G30610 42.953 447 233 9 27 463 30 464 7.61e-119 356
MS.gene50212.t1 AT2G24000 42.472 445 233 8 27 460 28 460 5.19e-117 351
MS.gene50212.t1 AT4G30810 41.901 463 250 9 6 460 10 461 2.05e-116 350
MS.gene50212.t1 AT5G08260 40.333 481 254 10 5 462 7 477 3.97e-116 350
MS.gene50212.t1 AT5G23210 41.176 459 232 10 28 461 50 495 4.86e-115 347
MS.gene50212.t1 AT2G24010 41.575 457 231 9 27 463 28 468 3.87e-114 344
MS.gene50212.t1 AT1G61130 41.538 455 244 9 15 457 12 456 3.95e-113 341
MS.gene50212.t1 AT2G35770 42.889 443 232 11 26 463 34 460 1.22e-112 340
MS.gene50212.t1 AT2G35780 41.818 440 236 9 27 461 27 451 1.64e-111 337
MS.gene50212.t1 AT3G07990 41.403 442 243 9 26 463 30 459 3.03e-111 337
MS.gene50212.t1 AT1G11080 41.020 451 233 9 27 457 48 485 3.99e-111 337
MS.gene50212.t1 AT1G11080 41.020 451 233 9 27 457 48 485 4.20e-111 337
MS.gene50212.t1 AT2G24010 39.167 480 231 10 29 463 1 464 2.95e-107 327
MS.gene50212.t1 AT4G15100 39.375 480 258 9 5 463 16 483 7.25e-106 323
MS.gene50212.t1 AT2G05850 40.090 444 241 8 27 463 61 486 2.93e-104 319
MS.gene50212.t1 AT3G52020 40.498 442 240 12 27 463 77 500 1.89e-102 315
MS.gene50212.t1 AT3G17180 39.294 453 246 10 28 463 37 477 1.40e-101 312
MS.gene50212.t1 AT3G52000 39.002 441 245 9 27 463 61 481 7.64e-101 310
MS.gene50212.t1 AT5G23210 40.338 414 210 9 28 416 50 451 1.89e-98 303
MS.gene50212.t1 AT3G52010 38.739 444 246 10 27 463 62 486 1.63e-97 302
MS.gene50212.t1 AT5G23210 38.631 409 213 10 78 461 5 400 7.45e-90 280
MS.gene50212.t1 AT5G23210 38.519 405 211 10 82 461 8 399 1.04e-88 276
MS.gene50212.t1 AT2G24010 40.327 367 188 7 29 375 1 356 5.13e-85 265
MS.gene50212.t1 AT3G17180 37.260 416 228 11 22 416 27 430 4.24e-82 261
MS.gene50212.t1 AT4G12910 32.613 509 279 16 1 460 4 497 1.21e-76 248
MS.gene50212.t1 AT5G23210 37.222 360 189 9 82 416 8 355 5.22e-72 232
MS.gene50212.t1 AT3G25420 31.429 490 280 11 16 460 16 494 1.80e-69 229
MS.gene50212.t1 AT4G12910 31.906 467 254 16 1 418 4 455 3.71e-63 212
MS.gene50212.t1 AT3G56540 50.802 187 89 3 7 192 57 241 1.42e-55 186
MS.gene50212.t1 AT1G73300 29.438 445 266 15 29 460 32 441 1.71e-51 181
MS.gene50212.t1 AT1G73300 29.438 445 266 15 29 460 32 441 1.71e-51 181
MS.gene50212.t1 AT2G24000 35.880 301 171 7 170 460 109 397 3.11e-51 179
MS.gene50212.t1 AT2G24000 56.667 90 38 1 27 115 28 117 4.94e-26 109
MS.gene50212.t1 AT1G33540 28.285 449 293 14 29 460 28 464 3.70e-51 180
MS.gene50212.t1 AT1G33540 28.372 430 280 13 47 460 4 421 2.53e-50 177
MS.gene50212.t1 AT2G22920 28.700 446 270 16 29 460 24 435 4.31e-50 177
MS.gene50212.t1 AT1G73270 28.959 442 272 15 29 460 32 441 1.51e-49 175
MS.gene50212.t1 AT1G73270 28.959 442 272 15 29 460 32 441 1.98e-49 175
MS.gene50212.t1 AT2G22980 28.027 446 267 15 29 460 25 430 2.74e-49 174
MS.gene50212.t1 AT5G09640 28.788 462 277 16 29 460 26 465 2.83e-49 175
MS.gene50212.t1 AT5G36180 28.315 445 271 14 29 460 32 441 2.87e-49 174
MS.gene50212.t1 AT1G73280 29.350 477 284 18 1 460 1 441 2.90e-49 174
MS.gene50212.t1 AT1G73290 28.281 442 273 14 29 460 31 438 7.50e-48 171
MS.gene50212.t1 AT1G73310 27.540 443 277 14 29 460 32 441 1.44e-47 170
MS.gene50212.t1 AT2G23000 28.054 442 280 15 29 460 24 437 1.45e-47 170
MS.gene50212.t1 AT2G23000 28.054 442 280 15 29 460 24 437 1.45e-47 170
MS.gene50212.t1 AT2G23010 28.755 466 286 17 10 460 3 437 1.75e-47 170
MS.gene50212.t1 AT2G22990 27.803 446 274 16 29 460 22 433 4.20e-47 169
MS.gene50212.t1 AT2G23010 28.541 466 287 17 10 460 3 437 6.74e-47 168
MS.gene50212.t1 AT3G12240 29.176 449 262 17 29 460 27 436 9.46e-47 167
MS.gene50212.t1 AT2G22970 28.378 444 272 15 29 460 24 433 1.65e-46 167
MS.gene50212.t1 AT5G22980 28.738 428 268 12 40 458 95 494 6.85e-46 167
MS.gene50212.t1 AT2G22980 28.266 421 249 14 29 435 25 406 8.46e-46 164
MS.gene50212.t1 AT3G45010 29.148 446 274 13 35 467 92 508 1.31e-45 166
MS.gene50212.t1 AT3G12230 28.795 448 265 16 29 460 26 435 1.75e-45 164
MS.gene50212.t1 AT2G22980 28.333 420 248 14 29 434 25 405 1.83e-45 164
MS.gene50212.t1 AT3G10450 28.315 445 271 15 29 460 28 437 3.05e-45 164
MS.gene50212.t1 AT3G12220 28.251 446 270 16 29 460 26 435 2.30e-44 161
MS.gene50212.t1 AT3G12203 27.650 434 279 11 33 460 12 416 4.76e-44 160
MS.gene50212.t1 AT3G12203 27.650 434 279 11 33 460 33 437 6.71e-44 160
MS.gene50212.t1 AT3G10450 27.934 426 260 14 47 460 4 394 2.31e-43 157
MS.gene50212.t1 AT3G12220 28.337 427 257 15 47 460 15 405 5.65e-43 157
MS.gene50212.t1 AT2G22990 27.857 420 256 15 29 434 22 408 1.70e-42 155
MS.gene50212.t1 AT2G22990 27.791 421 257 15 29 435 22 409 2.26e-42 156
MS.gene50212.t1 AT2G22970 28.401 419 255 14 29 435 24 409 2.61e-42 155
MS.gene50212.t1 AT2G22970 28.469 418 254 14 29 434 24 408 8.24e-42 154
MS.gene50212.t1 AT3G12203 27.696 408 261 10 58 460 1 379 1.28e-41 152
MS.gene50212.t1 AT2G22990 27.130 446 268 17 29 460 22 424 6.14e-41 152
MS.gene50212.t1 AT3G10450 28.333 420 254 14 29 435 28 413 6.81e-41 151
MS.gene50212.t1 AT2G22920 27.670 412 237 15 29 419 24 395 3.36e-40 149
MS.gene50212.t1 AT3G10410 27.391 460 278 13 18 464 85 501 8.51e-40 150
MS.gene50212.t1 AT3G10450 27.930 401 243 13 47 435 4 370 4.59e-39 145
MS.gene50212.t1 AT1G73310 26.617 402 252 13 29 419 32 401 1.54e-37 142
MS.gene50212.t1 AT1G73290 27.182 401 249 13 29 419 31 398 1.89e-37 142
MS.gene50212.t1 AT1G73280 31.773 299 182 10 1 286 1 290 2.95e-35 135
MS.gene50212.t1 AT2G22920 31.343 268 165 9 29 286 24 282 1.07e-34 132
MS.gene50212.t1 AT1G73290 33.186 226 136 6 29 244 31 251 6.54e-33 127
MS.gene50212.t1 AT2G22980 30.970 268 163 9 29 286 25 280 3.32e-32 126
MS.gene50212.t1 AT5G36180 26.316 361 225 11 107 460 13 339 8.39e-32 125
MS.gene50212.t1 AT5G36180 26.316 361 225 11 107 460 13 339 8.39e-32 125
MS.gene50212.t1 AT1G73280 27.147 361 222 12 107 460 13 339 9.08e-32 124
MS.gene50212.t1 AT2G22970 33.333 240 145 7 29 259 24 257 1.08e-31 124
MS.gene50212.t1 AT1G15000 36.464 181 108 3 37 214 29 205 2.07e-29 120
MS.gene50212.t1 AT1G73280 26.724 348 214 12 120 460 1 314 1.79e-28 115
MS.gene50212.t1 AT2G22990 25.641 351 220 13 118 460 2 319 4.31e-28 114
MS.gene50212.t1 AT2G22990 25.356 351 221 13 118 460 2 319 1.97e-27 112
MS.gene50212.t1 AT2G22980 25.767 326 196 11 118 435 2 289 3.08e-27 111
MS.gene50212.t1 AT5G36180 25.816 337 210 10 106 435 12 315 3.84e-27 111
MS.gene50212.t1 AT3G12220 31.193 218 135 6 29 236 26 238 6.98e-26 109
MS.gene50212.t1 AT5G22960 30.857 175 99 3 35 208 25 178 4.35e-24 99.8
MS.gene50212.t1 AT1G73310 24.765 319 204 10 106 419 12 299 1.42e-22 98.6
MS.gene50212.t1 AT5G36180 25.392 319 198 10 107 418 13 298 1.35e-21 95.9
MS.gene50212.t1 AT2G27920 27.170 265 148 9 42 284 33 274 8.46e-20 92.0
MS.gene50212.t1 AT2G22920 25.850 294 163 13 182 460 3 256 1.80e-17 82.4
MS.gene50212.t1 AT2G27920 26.364 220 123 7 83 284 4 202 4.89e-13 70.9

Find 114 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCTCACTCTTTGGCTTAA+TGG 0.090755 5.2:+60912019 MS.gene50212:CDS
GAAACTTCTCGAACCATTTC+AGG 0.181933 5.2:-60913169 None:intergenic
GAGCACCATATGATTATTTC+TGG 0.211487 5.2:+60913810 MS.gene50212:CDS
TCTCAATTCTTGTTCAACTA+TGG 0.261897 5.2:-60914017 None:intergenic
TTCAAAACCGTATTCCAGTA+TGG 0.267358 5.2:+60914628 MS.gene50212:CDS
AAAATAAACCCCTCACTCTT+TGG 0.284368 5.2:+60912011 MS.gene50212:CDS
AGGAGGTGCCTTTACTGAAT+TGG 0.300382 5.2:+60912710 MS.gene50212:CDS
CCATCACCAGTAGGATAAAA+TGG 0.305409 5.2:-60912735 None:intergenic
GGAATGATTTCAGATGAAAT+TGG 0.326242 5.2:+60913839 MS.gene50212:CDS
TCCAGAGCGTTGTTTCTTAC+CGG 0.327019 5.2:+60913203 MS.gene50212:CDS
GATCTGGTTGTTAGTCTTCC+AGG 0.337856 5.2:+60911884 MS.gene50212:CDS
TATTGACTGAATTCAACTTT+AGG 0.341208 5.2:-60911911 None:intergenic
CTCTTTATTGAGTCTCCTGC+TGG 0.357375 5.2:+60912922 MS.gene50212:CDS
TGCTTTCGTTCTTCCTGAAA+TGG 0.362996 5.2:+60913156 MS.gene50212:CDS
GGAGGTTGGGCAACTGAGTA+TGG 0.366756 5.2:+60915313 MS.gene50212:CDS
GGAGGTGCCTTTACTGAATT+GGG 0.367283 5.2:+60912711 MS.gene50212:CDS
GGACTGTAATCTTGAACTTC+AGG 0.367870 5.2:-60914941 None:intergenic
ATTGGAAGCATATTAATGTT+AGG 0.368350 5.2:-60914591 None:intergenic
TGAACAATCCGTTTGAGAAT+TGG 0.369737 5.2:-60914609 None:intergenic
GATTCCGTTGTGCCATTACT+AGG 0.370360 5.2:+60914885 MS.gene50212:CDS
AAATAATTACCTGGCCTTTG+TGG 0.380863 5.2:-60914980 None:intergenic
CTAACAACCAGATCCTCAAT+AGG 0.380884 5.2:-60911875 None:intergenic
CTCGTCACCGATGTTGTAAT+CGG 0.389457 5.2:-60912974 None:intergenic
TTTCAAATTGAACTTGTAAC+CGG 0.392444 5.2:-60913634 None:intergenic
AAAATTGATACCTCTATTCC+AGG 0.400614 5.2:-60912784 None:intergenic
GCTCTAGAAGGTTGTGCATA+TGG 0.402187 5.2:-60915382 None:intergenic
AAACAACGCTCTGGATTTGT+AGG 0.402525 5.2:-60913195 None:intergenic
GTACCAACTTGCCGTATTCA+TGG 0.415219 5.2:+60914248 MS.gene50212:CDS
ACAACAAGGCCTTCAATTGA+TGG 0.420335 5.2:+60915451 MS.gene50212:CDS
ATTGAAGGCCTTGTTGTGTT+TGG 0.423282 5.2:-60915445 None:intergenic
AGGGTACCCTATTGAGGATC+TGG 0.425537 5.2:+60911868 MS.gene50212:CDS
TTTATTCATCCATCAATTGA+AGG 0.427596 5.2:-60915460 None:intergenic
GTAATGGCACAACGGAATCT+TGG 0.430159 5.2:-60914881 None:intergenic
TCGAACGCTCATTCGTGAAC+TGG 0.435177 5.2:+60914911 MS.gene50212:CDS
CTTTGCAATCTGCTAGTTGA+AGG 0.438085 5.2:+60911848 MS.gene50212:CDS
AGACTCAATAAAGAGAAGAT+TGG 0.442329 5.2:-60912914 None:intergenic
TACGAAAGAATTCAATGTCC+TGG 0.445941 5.2:+60912766 MS.gene50212:CDS
AATTACCTGAAAACCCATAC+TGG 0.452422 5.2:-60914642 None:intergenic
CAACCTCCCTGAGGTTCAAA+AGG 0.453733 5.2:+60914210 MS.gene50212:CDS
TTGTGGAACCAAGCTCCATA+TGG 0.454668 5.2:-60914963 None:intergenic
AACAGATGCAGTGCTCTAGA+AGG 0.459934 5.2:-60915394 None:intergenic
GGTCCGGGTTGTTCTTCCGT+TGG 0.462034 5.2:+60912687 MS.gene50212:intron
GTTAACTTTCGCCACCGTGC+GGG 0.467382 5.2:+60915342 MS.gene50212:CDS
ATTGAGTCTCCTGCTGGAGT+TGG 0.467432 5.2:+60912928 MS.gene50212:CDS
ATATGCTTCCAATTCTCAAA+CGG 0.470413 5.2:+60914601 MS.gene50212:CDS
CTGCACATGGACCATGAATA+CGG 0.472089 5.2:-60914259 None:intergenic
CAGGCTACTAAAATAAGTAT+AGG 0.472450 5.2:+60914148 MS.gene50212:intron
AATTCAATGTCCTGGAATAG+AGG 0.472547 5.2:+60912774 MS.gene50212:CDS
TTCAAGATTACAGTCCCATA+TGG 0.472759 5.2:+60914948 MS.gene50212:CDS
CTATGTGTAGGTTGGAGGTT+GGG 0.476731 5.2:+60915300 MS.gene50212:intron
CCGGGTTGTTCTTCCGTTGG+AGG 0.476735 5.2:+60912690 MS.gene50212:CDS
AATTCTTGTTCAACTATGGA+TGG 0.481844 5.2:-60914013 None:intergenic
CTGTTCAGTAACTTTGTGAA+TGG 0.483667 5.2:+60915412 MS.gene50212:CDS
TGGACCTGCATAGCTTTCTC+CGG 0.483807 5.2:-60913222 None:intergenic
CATGATGCAGATACATTATG+AGG 0.484701 5.2:-60913911 None:intergenic
AACTAGCAGATTGCAAAGAT+AGG 0.487129 5.2:-60911843 None:intergenic
GAATTACAATGGCACAGTAT+TGG 0.487226 5.2:+60911795 None:intergenic
GAAGAACGAAAGCATATCAT+TGG 0.488029 5.2:-60913147 None:intergenic
TCCGGTAAGAAACAACGCTC+TGG 0.495399 5.2:-60913204 None:intergenic
TGTGACCAGAAATAATCATA+TGG 0.497544 5.2:-60913815 None:intergenic
TCAGTAACTTTGTGAATGGT+AGG 0.501716 5.2:+60915416 MS.gene50212:CDS
TATCCTACTGGTGATGGACG+TGG 0.506127 5.2:+60912741 MS.gene50212:CDS
GGTTGTTCTTCCGTTGGAGG+AGG 0.508574 5.2:+60912693 MS.gene50212:CDS
TGAAAACCCATACTGGAATA+CGG 0.509668 5.2:-60914635 None:intergenic
TCAAAACCGTATTCCAGTAT+GGG 0.511117 5.2:+60914629 MS.gene50212:CDS
GGTTACAAGTTCAATTTGAA+AGG 0.512302 5.2:+60913636 MS.gene50212:CDS
GTTGAATTCAGTCAATATGC+TGG 0.514110 5.2:+60911917 MS.gene50212:CDS
TCCATTAAGCCAAAGAGTGA+GGG 0.516289 5.2:-60912020 None:intergenic
TTACAGTCCCATATGGAGCT+TGG 0.523649 5.2:+60914955 MS.gene50212:CDS
CTTACCGGAGAAAGCTATGC+AGG 0.524600 5.2:+60913218 MS.gene50212:CDS
AGTGATGCAAATGAAGTTGT+TGG 0.524835 5.2:+60913947 MS.gene50212:CDS
TGTGGAACCAAGCTCCATAT+GGG 0.530718 5.2:-60914962 None:intergenic
TAACAACCAGATCCTCAATA+GGG 0.533256 5.2:-60911874 None:intergenic
CATATCTATGTGTAGGTTGG+AGG 0.535597 5.2:+60915295 MS.gene50212:intron
AGTCTCCTGCTGGAGTTGGT+TGG 0.541071 5.2:+60912932 MS.gene50212:CDS
AAATGGACCCAATTCAGTAA+AGG 0.548629 5.2:-60912718 None:intergenic
AGTTGAAGGGTACCCTATTG+AGG 0.549321 5.2:+60911862 MS.gene50212:CDS
TTAACTTTCGCCACCGTGCG+GGG 0.550282 5.2:+60915343 MS.gene50212:CDS
TTTGCAATCTGCTAGTTGAA+GGG 0.556691 5.2:+60911849 MS.gene50212:CDS
TTATACTTACCCACTGCACA+TGG 0.557753 5.2:-60914272 None:intergenic
GCAAGTTGGTACGGTTCGCA+TGG 0.558199 5.2:-60914237 None:intergenic
TCTATGTGTAGGTTGGAGGT+TGG 0.559649 5.2:+60915299 MS.gene50212:intron
AACTTCATTTGCATCACTGA+TGG 0.561383 5.2:-60913942 None:intergenic
TATGACCAACCAACTCCAGC+AGG 0.564584 5.2:-60912937 None:intergenic
TATTCATGGTCCATGTGCAG+TGG 0.566051 5.2:+60914262 MS.gene50212:CDS
CTCGATCCAGATTCAAAAGT+GGG 0.567933 5.2:-60913781 None:intergenic
CGTGCGGGGTGCATCTCACA+TGG 0.568257 5.2:+60915357 MS.gene50212:CDS
AATTCAACTTTAGGTTGACC+TGG 0.576320 5.2:-60911902 None:intergenic
AGCTTGGTTCCACAAAGGCC+AGG 0.580658 5.2:+60914971 MS.gene50212:CDS
ATGAATACGGCAAGTTGGTA+CGG 0.581357 5.2:-60914246 None:intergenic
CTCCATTAAGCCAAAGAGTG+AGG 0.581909 5.2:-60912021 None:intergenic
CGATTACAACATCGGTGACG+AGG 0.590138 5.2:+60912975 MS.gene50212:CDS
TCTCGATCCAGATTCAAAAG+TGG 0.599175 5.2:-60913782 None:intergenic
CCTCCAACGGAAGAACAACC+CGG 0.601058 5.2:-60912690 None:intergenic
TATGATTATTTCTGGTCACA+TGG 0.601807 5.2:+60913818 MS.gene50212:CDS
CTCACTCTTTGGCTTAATGG+AGG 0.603231 5.2:+60912022 MS.gene50212:CDS
GGACCATGAATACGGCAAGT+TGG 0.604501 5.2:-60914251 None:intergenic
AGTAAAGGCACCTCCTCCAA+CGG 0.608931 5.2:-60912703 None:intergenic
TACATTATGAGGAGATGCGA+AGG 0.615123 5.2:-60913900 None:intergenic
ATTCATGGTCCATGTGCAGT+GGG 0.615927 5.2:+60914263 MS.gene50212:CDS
TGTTAACTTTCGCCACCGTG+CGG 0.616872 5.2:+60915341 MS.gene50212:CDS
AACGCTCTGGATTTGTAGGT+AGG 0.617990 5.2:-60913191 None:intergenic
ACAACTTCCGATTACAACAT+CGG 0.619469 5.2:+60912967 MS.gene50212:CDS
AACATCGGTGACGAGGCCAC+AGG 0.633390 5.2:+60912982 MS.gene50212:CDS
CCATTAAGCCAAAGAGTGAG+GGG 0.635706 5.2:-60912019 None:intergenic
AGCGTTCGAGAACCTAGTAA+TGG 0.641706 5.2:-60914897 None:intergenic
GGAGATTGCCAAACACAACA+AGG 0.641848 5.2:+60915437 MS.gene50212:CDS
AGAATAGCATTAGCTAACTG+AGG 0.658804 5.2:-60913591 None:intergenic
CATGTGAGATGCACCCCGCA+CGG 0.659786 5.2:-60915356 None:intergenic
GTTGATATTGATGTAAAACA+TGG 0.675625 5.2:+60911944 MS.gene50212:CDS
AAACCACGTCCATCACCAGT+AGG 0.679633 5.2:-60912744 None:intergenic
TATGGAGCTTGGTTCCACAA+AGG 0.680414 5.2:+60914966 MS.gene50212:CDS
GTGAGATGCACCCCGCACGG+TGG 0.715495 5.2:-60915353 None:intergenic
AGAACCTAGTAATGGCACAA+CGG 0.721630 5.2:-60914889 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAATAATTAAAATT+GGG - chr5.2:60913267-60913286 None:intergenic 0.0%
!!! TTTTTTTTTTTTTTTTTTTT+TGG + chr5.2:60912147-60912166 MS.gene50212:intron 0.0%
!! AATATAAGATATCATATGAA+TGG - chr5.2:60913677-60913696 None:intergenic 15.0%
!! AGATAATAAAAATAGAAAAG+AGG - chr5.2:60913491-60913510 None:intergenic 15.0%
!! ATACTTTATAATGTTTATTC+AGG + chr5.2:60913558-60913577 MS.gene50212:intron 15.0%
!! ATATAAGATATCATATGAAT+GGG - chr5.2:60913676-60913695 None:intergenic 15.0%
!! ATTGATATTAGAGAATAATT+TGG + chr5.2:60912835-60912854 MS.gene50212:intron 15.0%
!! GATAAAGTATAGTATTTATA+GGG - chr5.2:60914719-60914738 None:intergenic 15.0%
!! TGATAAAGTATAGTATTTAT+AGG - chr5.2:60914720-60914739 None:intergenic 15.0%
!! TTCATATGATATCTTATATT+TGG + chr5.2:60913677-60913696 MS.gene50212:intron 15.0%
!! TTGATATTAGAGAATAATTT+GGG + chr5.2:60912836-60912855 MS.gene50212:intron 15.0%
!!! ATAATCCTTTAATTTGTTTT+TGG + chr5.2:60913121-60913140 MS.gene50212:intron 15.0%
!! AACTGTTAAAAATAATTACC+TGG - chr5.2:60914992-60915011 None:intergenic 20.0%
!! AGTGTTTAATTAAGAAATCT+GGG + chr5.2:60913706-60913725 MS.gene50212:intron 20.0%
!! GTAAACACAATATTAAAATC+AGG - chr5.2:60912876-60912895 None:intergenic 20.0%
!! GTATAAATTAAATAGCCAAT+TGG - chr5.2:60914685-60914704 None:intergenic 20.0%
!! TAAAATTCAAATTGCATGTT+TGG - chr5.2:60912284-60912303 None:intergenic 20.0%
!! TAGTATAATAGAAATGAAAG+AGG - chr5.2:60914066-60914085 None:intergenic 20.0%
!!! ACTCTTACTTTTGATTATTA+TGG + chr5.2:60912075-60912094 MS.gene50212:intron 20.0%
!!! AGTAACTTTGATAATTTTTC+AGG + chr5.2:60912666-60912685 MS.gene50212:intron 20.0%
!!! GGTATCAATTTTGTTTATTA+TGG + chr5.2:60912795-60912814 MS.gene50212:intron 20.0%
!!! TTTGTTAGTTTTATAATGCA+TGG + chr5.2:60913096-60913115 MS.gene50212:intron 20.0%
!!! TTTTACATGTTGTTATCTTT+TGG + chr5.2:60912299-60912318 MS.gene50212:intron 20.0%
! AAGTTCAGTATTCTATAGTA+TGG + chr5.2:60915038-60915057 MS.gene50212:intron 25.0%
! AGAATTGAGATTGAAAAAGA+TGG + chr5.2:60914030-60914049 MS.gene50212:CDS 25.0%
! AGTATAGCATCTTTCATATA+AGG - chr5.2:60912530-60912549 None:intergenic 25.0%
! CCAAGAATTGTAGAAAATAA+TGG - chr5.2:60915235-60915254 None:intergenic 25.0%
! GAGTGTTTAATTAAGAAATC+TGG + chr5.2:60913705-60913724 MS.gene50212:intron 25.0%
! GTGTTAAATTATTATGACAG+TGG + chr5.2:60914855-60914874 MS.gene50212:intron 25.0%
! GTTGATATTGATGTAAAACA+TGG + chr5.2:60911944-60911963 MS.gene50212:CDS 25.0%
! TCGAAAAAAATCAACATGTA+AGG - chr5.2:60914402-60914421 None:intergenic 25.0%
! TGTCGCTAATAATAGTTTAT+GGG - chr5.2:60914315-60914334 None:intergenic 25.0%
! TTTCAAATTGAACTTGTAAC+CGG - chr5.2:60913637-60913656 None:intergenic 25.0%
!! ATTGGAAGCATATTAATGTT+AGG - chr5.2:60914594-60914613 None:intergenic 25.0%
!! CCATTATTTTCTACAATTCT+TGG + chr5.2:60915232-60915251 MS.gene50212:intron 25.0%
!! TATTGACTGAATTCAACTTT+AGG - chr5.2:60911914-60911933 None:intergenic 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG - chr5.2:60915018-60915037 None:intergenic 25.0%
AAAATCGTTCATGAGAACAA+AGG - chr5.2:60913525-60913544 None:intergenic 30.0%
AAAATTGATACCTCTATTCC+AGG - chr5.2:60912787-60912806 None:intergenic 30.0%
AAAGTTTAAATCAGTACCTG+TGG - chr5.2:60913001-60913020 None:intergenic 30.0%
AATTCTTGTTCAACTATGGA+TGG - chr5.2:60914016-60914035 None:intergenic 30.0%
ACAACACAATCTGAATTTGT+AGG - chr5.2:60912389-60912408 None:intergenic 30.0%
AGACTCAATAAAGAGAAGAT+TGG - chr5.2:60912917-60912936 None:intergenic 30.0%
ATATGCTTCCAATTCTCAAA+CGG + chr5.2:60914601-60914620 MS.gene50212:CDS 30.0%
CAAATTCAAATCCTGTTACA+AGG + chr5.2:60912608-60912627 MS.gene50212:intron 30.0%
CAACACAATCTGAATTTGTA+GGG - chr5.2:60912388-60912407 None:intergenic 30.0%
CAGGCTACTAAAATAAGTAT+AGG + chr5.2:60914148-60914167 MS.gene50212:intron 30.0%
GCATCTTTCATATAAGGTAA+AGG - chr5.2:60912524-60912543 None:intergenic 30.0%
GGAATGATTTCAGATGAAAT+TGG + chr5.2:60913839-60913858 MS.gene50212:CDS 30.0%
GGTTACAAGTTCAATTTGAA+AGG + chr5.2:60913636-60913655 MS.gene50212:CDS 30.0%
GTTTCGAAATTCTCAAAAGT+TGG + chr5.2:60913288-60913307 MS.gene50212:intron 30.0%
TCGTCATTGTTGTCAAATAA+AGG + chr5.2:60915106-60915125 MS.gene50212:intron 30.0%
TCTCAATTCTTGTTCAACTA+TGG - chr5.2:60914020-60914039 None:intergenic 30.0%
TGCATAAAGAATACAATACG+AGG - chr5.2:60913373-60913392 None:intergenic 30.0%
TGTGACCAGAAATAATCATA+TGG - chr5.2:60913818-60913837 None:intergenic 30.0%
TTATGAGATACGCTATTTAG+CGG + chr5.2:60915175-60915194 MS.gene50212:intron 30.0%
TTGATTGCATATCTATGTGT+AGG + chr5.2:60915288-60915307 MS.gene50212:intron 30.0%
TTTCAACAAAAGAGACCAAT+TGG + chr5.2:60914667-60914686 MS.gene50212:intron 30.0%
TTTGACAAGAACAGAATCTA+TGG + chr5.2:60912443-60912462 MS.gene50212:intron 30.0%
! AAAATCGCAGTCTTTTGTAA+TGG - chr5.2:60913870-60913889 None:intergenic 30.0%
! AAGACGATATCTCTAGTTTA+TGG - chr5.2:60914346-60914365 None:intergenic 30.0%
! AGACGATATCTCTAGTTTAT+GGG - chr5.2:60914345-60914364 None:intergenic 30.0%
! ATTAGCTTCTTAGTAATCAG+AGG - chr5.2:60914832-60914851 None:intergenic 30.0%
! GATCTGATTAATGTGTCTTT+TGG - chr5.2:60913245-60913264 None:intergenic 30.0%
! GTGTCGCTAATAATAGTTTA+TGG - chr5.2:60914316-60914335 None:intergenic 30.0%
! TGTAACTTTTGTCTAAATCG+CGG + chr5.2:60912231-60912250 MS.gene50212:intron 30.0%
!! GATTTTAATGCTCATTCAAC+CGG + chr5.2:60913615-60913634 MS.gene50212:CDS 30.0%
!! TATGATTATTTCTGGTCACA+TGG + chr5.2:60913818-60913837 MS.gene50212:CDS 30.0%
!!! CTTTGATAATTTTTCAGGTC+CGG + chr5.2:60912671-60912690 MS.gene50212:intron 30.0%
!!! GTAATCTAAGCTTTTTTCTC+TGG + chr5.2:60913741-60913760 MS.gene50212:intron 30.0%
!!! TTTGATAATTTTTCAGGTCC+GGG + chr5.2:60912672-60912691 MS.gene50212:intron 30.0%
!!! TTTTTGGTTCTGTGTATGTT+TGG + chr5.2:60912164-60912183 MS.gene50212:intron 30.0%
AAAATAAACCCCTCACTCTT+TGG + chr5.2:60912011-60912030 MS.gene50212:CDS 35.0%
AAATGGACCCAATTCAGTAA+AGG - chr5.2:60912721-60912740 None:intergenic 35.0%
AACTAGCAGATTGCAAAGAT+AGG - chr5.2:60911846-60911865 None:intergenic 35.0%
AACTTCATTTGCATCACTGA+TGG - chr5.2:60913945-60913964 None:intergenic 35.0%
AATATCCCAGTTAGCAATGA+TGG - chr5.2:60913413-60913432 None:intergenic 35.0%
AATTACCTGAAAACCCATAC+TGG - chr5.2:60914645-60914664 None:intergenic 35.0%
AATTCAATGTCCTGGAATAG+AGG + chr5.2:60912774-60912793 MS.gene50212:CDS 35.0%
ACAACTTCCGATTACAACAT+CGG + chr5.2:60912967-60912986 MS.gene50212:CDS 35.0%
AGAATAGCATTAGCTAACTG+AGG - chr5.2:60913594-60913613 None:intergenic 35.0%
AGTGATGCAAATGAAGTTGT+TGG + chr5.2:60913947-60913966 MS.gene50212:CDS 35.0%
CATGATGCAGATACATTATG+AGG - chr5.2:60913914-60913933 None:intergenic 35.0%
CTGTTCAGTAACTTTGTGAA+TGG + chr5.2:60915412-60915431 MS.gene50212:CDS 35.0%
GAGAGTGAGTAAAACTTGTA+AGG - chr5.2:60914772-60914791 None:intergenic 35.0%
GAGCACCATATGATTATTTC+TGG + chr5.2:60913810-60913829 MS.gene50212:CDS 35.0%
GGCTGCCAAAAACAAATTAA+AGG - chr5.2:60913129-60913148 None:intergenic 35.0%
GTTGAATTCAGTCAATATGC+TGG + chr5.2:60911917-60911936 MS.gene50212:CDS 35.0%
TAACAACCAGATCCTCAATA+GGG - chr5.2:60911877-60911896 None:intergenic 35.0%
TACGAAAGAATTCAATGTCC+TGG + chr5.2:60912766-60912785 MS.gene50212:CDS 35.0%
TCAAAACCGTATTCCAGTAT+GGG + chr5.2:60914629-60914648 MS.gene50212:CDS 35.0%
TCAGTAACTTTGTGAATGGT+AGG + chr5.2:60915416-60915435 MS.gene50212:CDS 35.0%
TCTGTGTATGTTTGGATTGA+CGG + chr5.2:60912172-60912191 MS.gene50212:intron 35.0%
TGAAAACCCATACTGGAATA+CGG - chr5.2:60914638-60914657 None:intergenic 35.0%
TGAACAATCCGTTTGAGAAT+TGG - chr5.2:60914612-60914631 None:intergenic 35.0%
TTCAAAACCGTATTCCAGTA+TGG + chr5.2:60914628-60914647 MS.gene50212:CDS 35.0%
TTCAAGATTACAGTCCCATA+TGG + chr5.2:60914948-60914967 MS.gene50212:CDS 35.0%
TTGCATATCTATGTGTAGGT+TGG + chr5.2:60915292-60915311 MS.gene50212:intron 35.0%
TTTGCAATCTGCTAGTTGAA+GGG + chr5.2:60911849-60911868 MS.gene50212:CDS 35.0%
! AAATAATTACCTGGCCTTTG+TGG - chr5.2:60914983-60915002 None:intergenic 35.0%
! ACTCCAGTTTTCCAAATCAA+CGG + chr5.2:60915077-60915096 MS.gene50212:intron 35.0%
! GAAGAACGAAAGCATATCAT+TGG - chr5.2:60913150-60913169 None:intergenic 35.0%
! TACAAGTTTTACTCACTCTC+AGG + chr5.2:60914772-60914791 MS.gene50212:intron 35.0%
!! AATTCAACTTTAGGTTGACC+TGG - chr5.2:60911905-60911924 None:intergenic 35.0%
!! GCTACAACATTGTAGTTTTG+CGG + chr5.2:60915199-60915218 MS.gene50212:intron 35.0%
!! TTTCTCTGGCTTTGTTAGAT+TGG + chr5.2:60913755-60913774 MS.gene50212:intron 35.0%
ACAACAAGGCCTTCAATTGA+TGG + chr5.2:60915451-60915470 MS.gene50212:CDS 40.0%
AGAACCTAGTAATGGCACAA+CGG - chr5.2:60914892-60914911 None:intergenic 40.0%
CCATCACCAGTAGGATAAAA+TGG - chr5.2:60912738-60912757 None:intergenic 40.0%
CGGTGAGTTTACCAAAATCA+CGG + chr5.2:60912192-60912211 MS.gene50212:intron 40.0%
CTAACAACCAGATCCTCAAT+AGG - chr5.2:60911878-60911897 None:intergenic 40.0%
CTATGCCATCATTGCTAACT+GGG + chr5.2:60913405-60913424 MS.gene50212:intron 40.0%
CTCGATCCAGATTCAAAAGT+GGG - chr5.2:60913784-60913803 None:intergenic 40.0%
CTTTGCAATCTGCTAGTTGA+AGG + chr5.2:60911848-60911867 MS.gene50212:CDS 40.0%
GAAACTTCTCGAACCATTTC+AGG - chr5.2:60913172-60913191 None:intergenic 40.0%
GGACTGTAATCTTGAACTTC+AGG - chr5.2:60914944-60914963 None:intergenic 40.0%
TACATTATGAGGAGATGCGA+AGG - chr5.2:60913903-60913922 None:intergenic 40.0%
TCCATTAAGCCAAAGAGTGA+GGG - chr5.2:60912023-60912042 None:intergenic 40.0%
TCTCGATCCAGATTCAAAAG+TGG - chr5.2:60913785-60913804 None:intergenic 40.0%
TGCTTTCGTTCTTCCTGAAA+TGG + chr5.2:60913156-60913175 MS.gene50212:CDS 40.0%
TTATACTTACCCACTGCACA+TGG - chr5.2:60914275-60914294 None:intergenic 40.0%
TTCGTCTATTTCGTTACTGC+AGG + chr5.2:60914129-60914148 MS.gene50212:intron 40.0%
! AAACAACGCTCTGGATTTGT+AGG - chr5.2:60913198-60913217 None:intergenic 40.0%
! AACTTTTGTCTAAATCGCGG+TGG + chr5.2:60912234-60912253 MS.gene50212:intron 40.0%
! ATTTTACTTCAACCTCCCTG+AGG + chr5.2:60914201-60914220 MS.gene50212:CDS 40.0%
! CAGATTATGAGCCTTGTAAC+AGG - chr5.2:60912622-60912641 None:intergenic 40.0%
! CATATCTATGTGTAGGTTGG+AGG + chr5.2:60915295-60915314 MS.gene50212:intron 40.0%
! CCATTTTATCCTACTGGTGA+TGG + chr5.2:60912735-60912754 MS.gene50212:CDS 40.0%
! GACGAAAATCACCGTTGATT+TGG - chr5.2:60915091-60915110 None:intergenic 40.0%
! GTATTCCAGTATGGGTTTTC+AGG + chr5.2:60914637-60914656 MS.gene50212:CDS 40.0%
! TCACAGTACAACCGTGATTT+TGG - chr5.2:60912206-60912225 None:intergenic 40.0%
! TTGGGTCCATTTTATCCTAC+TGG + chr5.2:60912729-60912748 MS.gene50212:CDS 40.0%
!! ATGAATACGGCAAGTTGGTA+CGG - chr5.2:60914249-60914268 None:intergenic 40.0%
!! ATTGAAGGCCTTGTTGTGTT+TGG - chr5.2:60915448-60915467 None:intergenic 40.0%
!! TCACCGTTGATTTGGAAAAC+TGG - chr5.2:60915083-60915102 None:intergenic 40.0%
!!! AGAGTGAGGGGTTTATTTTG+AGG - chr5.2:60912010-60912029 None:intergenic 40.0%
AGCGTTCGAGAACCTAGTAA+TGG - chr5.2:60914900-60914919 None:intergenic 45.0%
ATAGTGACGCTATTGCGTAG+TGG + chr5.2:60915131-60915150 MS.gene50212:intron 45.0%
ATTCATGGTCCATGTGCAGT+GGG + chr5.2:60914263-60914282 MS.gene50212:CDS 45.0%
CCAGTTAGCAATGATGGCAT+AGG - chr5.2:60913407-60913426 None:intergenic 45.0%
CCATTAAGCCAAAGAGTGAG+GGG - chr5.2:60912022-60912041 None:intergenic 45.0%
CCTATGCCATCATTGCTAAC+TGG + chr5.2:60913404-60913423 MS.gene50212:intron 45.0%
CTCCATTAAGCCAAAGAGTG+AGG - chr5.2:60912024-60912043 None:intergenic 45.0%
CTCGTCACCGATGTTGTAAT+CGG - chr5.2:60912977-60912996 None:intergenic 45.0%
CTCTTTATTGAGTCTCCTGC+TGG + chr5.2:60912922-60912941 MS.gene50212:CDS 45.0%
CTGCACATGGACCATGAATA+CGG - chr5.2:60914262-60914281 None:intergenic 45.0%
GGAGATTGCCAAACACAACA+AGG + chr5.2:60915437-60915456 MS.gene50212:CDS 45.0%
GTAATGGCACAACGGAATCT+TGG - chr5.2:60914884-60914903 None:intergenic 45.0%
GTACCAACTTGCCGTATTCA+TGG + chr5.2:60914248-60914267 MS.gene50212:CDS 45.0%
TATGGAGCTTGGTTCCACAA+AGG + chr5.2:60914966-60914985 MS.gene50212:CDS 45.0%
TATTCATGGTCCATGTGCAG+TGG + chr5.2:60914262-60914281 MS.gene50212:CDS 45.0%
TGTGGAACCAAGCTCCATAT+GGG - chr5.2:60914965-60914984 None:intergenic 45.0%
TTACAGTCCCATATGGAGCT+TGG + chr5.2:60914955-60914974 MS.gene50212:CDS 45.0%
TTGTGGAACCAAGCTCCATA+TGG - chr5.2:60914966-60914985 None:intergenic 45.0%
! AACAGATGCAGTGCTCTAGA+AGG - chr5.2:60915397-60915416 None:intergenic 45.0%
! AACGCTCTGGATTTGTAGGT+AGG - chr5.2:60913194-60913213 None:intergenic 45.0%
! CGCAGTCTTTTGTAATGGCA+AGG - chr5.2:60913865-60913884 None:intergenic 45.0%
! CTATGTGTAGGTTGGAGGTT+GGG + chr5.2:60915300-60915319 MS.gene50212:intron 45.0%
! CTCACTCTTTGGCTTAATGG+AGG + chr5.2:60912022-60912041 MS.gene50212:CDS 45.0%
! GATTCCGTTGTGCCATTACT+AGG + chr5.2:60914885-60914904 MS.gene50212:CDS 45.0%
! TCTATGTGTAGGTTGGAGGT+TGG + chr5.2:60915299-60915318 MS.gene50212:intron 45.0%
!! AGGAGGTGCCTTTACTGAAT+TGG + chr5.2:60912710-60912729 MS.gene50212:CDS 45.0%
!! AGTTGAAGGGTACCCTATTG+AGG + chr5.2:60911862-60911881 MS.gene50212:CDS 45.0%
!! GATCTGGTTGTTAGTCTTCC+AGG + chr5.2:60911884-60911903 MS.gene50212:CDS 45.0%
!! GCTCTAGAAGGTTGTGCATA+TGG - chr5.2:60915385-60915404 None:intergenic 45.0%
!! GGAGGTGCCTTTACTGAATT+GGG + chr5.2:60912711-60912730 MS.gene50212:CDS 45.0%
!! TCCAGAGCGTTGTTTCTTAC+CGG + chr5.2:60913203-60913222 MS.gene50212:CDS 45.0%
!! TGGCAACCCACTTTTGAATC+TGG + chr5.2:60913775-60913794 MS.gene50212:CDS 45.0%
!! GATAAATTAATAATTAAAAT+TGG - chr5.2:60913268-60913287 None:intergenic 5.0%
AAACCACGTCCATCACCAGT+AGG - chr5.2:60912747-60912766 None:intergenic 50.0%
ATTGAGTCTCCTGCTGGAGT+TGG + chr5.2:60912928-60912947 MS.gene50212:CDS 50.0%
CAACCTCCCTGAGGTTCAAA+AGG + chr5.2:60914210-60914229 MS.gene50212:CDS 50.0%
CGATTACAACATCGGTGACG+AGG + chr5.2:60912975-60912994 MS.gene50212:CDS 50.0%
CTTACCGGAGAAAGCTATGC+AGG + chr5.2:60913218-60913237 MS.gene50212:CDS 50.0%
GGACCATGAATACGGCAAGT+TGG - chr5.2:60914254-60914273 None:intergenic 50.0%
TATGACCAACCAACTCCAGC+AGG - chr5.2:60912940-60912959 None:intergenic 50.0%
TCCGGTAAGAAACAACGCTC+TGG - chr5.2:60913207-60913226 None:intergenic 50.0%
TCGAACGCTCATTCGTGAAC+TGG + chr5.2:60914911-60914930 MS.gene50212:CDS 50.0%
TGGACCTGCATAGCTTTCTC+CGG - chr5.2:60913225-60913244 None:intergenic 50.0%
TGTTAACTTTCGCCACCGTG+CGG + chr5.2:60915341-60915360 MS.gene50212:CDS 50.0%
! AGGGTACCCTATTGAGGATC+TGG + chr5.2:60911868-60911887 MS.gene50212:CDS 50.0%
! CCCCTCACTCTTTGGCTTAA+TGG + chr5.2:60912019-60912038 MS.gene50212:CDS 50.0%
! GGAGAGCCTTTTGAACCTCA+GGG - chr5.2:60914219-60914238 None:intergenic 50.0%
! TGGAGAGCCTTTTGAACCTC+AGG - chr5.2:60914220-60914239 None:intergenic 50.0%
!! AGTAAAGGCACCTCCTCCAA+CGG - chr5.2:60912706-60912725 None:intergenic 50.0%
!! TATCCTACTGGTGATGGACG+TGG + chr5.2:60912741-60912760 MS.gene50212:CDS 50.0%
AGCTTGGTTCCACAAAGGCC+AGG + chr5.2:60914971-60914990 MS.gene50212:CDS 55.0%
CCTCCAACGGAAGAACAACC+CGG - chr5.2:60912693-60912712 None:intergenic 55.0%
GGAGGTTGGGCAACTGAGTA+TGG + chr5.2:60915313-60915332 MS.gene50212:CDS 55.0%
GGTTGTTCTTCCGTTGGAGG+AGG + chr5.2:60912693-60912712 MS.gene50212:CDS 55.0%
GTTAACTTTCGCCACCGTGC+GGG + chr5.2:60915342-60915361 MS.gene50212:CDS 55.0%
TTAACTTTCGCCACCGTGCG+GGG + chr5.2:60915343-60915362 MS.gene50212:CDS 55.0%
! AGTCTCCTGCTGGAGTTGGT+TGG + chr5.2:60912932-60912951 MS.gene50212:CDS 55.0%
! GAGCCTTTTGAACCTCAGGG+AGG - chr5.2:60914216-60914235 None:intergenic 55.0%
!! GCAAGTTGGTACGGTTCGCA+TGG - chr5.2:60914240-60914259 None:intergenic 55.0%
AACATCGGTGACGAGGCCAC+AGG + chr5.2:60912982-60913001 MS.gene50212:CDS 60.0%
CATGTGAGATGCACCCCGCA+CGG - chr5.2:60915359-60915378 None:intergenic 60.0%
CCGGGTTGTTCTTCCGTTGG+AGG + chr5.2:60912690-60912709 MS.gene50212:CDS 60.0%
GGTCCGGGTTGTTCTTCCGT+TGG + chr5.2:60912687-60912706 MS.gene50212:intron 60.0%
CGTGCGGGGTGCATCTCACA+TGG + chr5.2:60915357-60915376 MS.gene50212:CDS 65.0%
GTGAGATGCACCCCGCACGG+TGG - chr5.2:60915356-60915375 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 60911803 60915477 60911803 ID=MS.gene50212
chr5.2 mRNA 60911803 60915477 60911803 ID=MS.gene50212.t1;Parent=MS.gene50212
chr5.2 exon 60911803 60912043 60911803 ID=MS.gene50212.t1.exon1;Parent=MS.gene50212.t1
chr5.2 CDS 60911803 60912043 60911803 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60912688 60912795 60912688 ID=MS.gene50212.t1.exon2;Parent=MS.gene50212.t1
chr5.2 CDS 60912688 60912795 60912688 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60912911 60913003 60912911 ID=MS.gene50212.t1.exon3;Parent=MS.gene50212.t1
chr5.2 CDS 60912911 60913003 60912911 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60913147 60913239 60913147 ID=MS.gene50212.t1.exon4;Parent=MS.gene50212.t1
chr5.2 CDS 60913147 60913239 60913147 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60913580 60913665 60913580 ID=MS.gene50212.t1.exon5;Parent=MS.gene50212.t1
chr5.2 CDS 60913580 60913665 60913580 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60913773 60914051 60913773 ID=MS.gene50212.t1.exon6;Parent=MS.gene50212.t1
chr5.2 CDS 60913773 60914051 60913773 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60914151 60914284 60914151 ID=MS.gene50212.t1.exon7;Parent=MS.gene50212.t1
chr5.2 CDS 60914151 60914284 60914151 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60914566 60914658 60914566 ID=MS.gene50212.t1.exon8;Parent=MS.gene50212.t1
chr5.2 CDS 60914566 60914658 60914566 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60914875 60914992 60914875 ID=MS.gene50212.t1.exon9;Parent=MS.gene50212.t1
chr5.2 CDS 60914875 60914992 60914875 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
chr5.2 exon 60915310 60915477 60915310 ID=MS.gene50212.t1.exon10;Parent=MS.gene50212.t1
chr5.2 CDS 60915310 60915477 60915310 ID=cds.MS.gene50212.t1;Parent=MS.gene50212.t1
Gene Sequence

>MS.gene50212

ATGGCACAGTATTGGTTTCTTAGTGTATTGATTGCTGTGTCCTATCTTTGCAATCTGCTAGTTGAAGGGTACCCTATTGAGGATCTGGTTGTTAGTCTTCCAGGTCAACCTAAAGTTGAATTCAGTCAATATGCTGGTTATGTTGATATTGATGTAAAACATGGTAGAAGTTTGTTTTATTATTTTGTTGAAGCTGATCATAAACCTCAAAATAAACCCCTCACTCTTTGGCTTAATGGAGGTAAATAATAATTCACTAATTATATATCATCACTCTTACTTTTGATTATTATGGTTGCTGACTTAATTTTCTTGTTATATTATTTTCAGTATATTTTGTTATCTTTTTTTTTTTTTTTTTTTTTTGGTTCTGTGTATGTTTGGATTGACGGTGAGTTTACCAAAATCACGGTTGTACTGTGATTTAGTGTAACTTTTGTCTAAATCGCGGTGGTTCAGCGTGATTTTGACAATTAGACCAAACATGCAATTTGAATTTTACATGTTGTTATCTTTTGGTGAAAAGCATTGTACTTGTGTTGTTTTGTGTGTGTTGTTTTGTGTTATGTTATGTTGTGTTGTCCCTACAAATTCAGATTGTGTTGTTGTTCTTGTCTTTATAGTACAAAATCATGTTTTCTTTGACAAGAACAGAATCTATGGATCAAAAATGTTGAATGATTCTGCTTTTCTAACAGTTGACAATTGACAGAAAGTACCTTTACCTTATATGAAAGATGCTATACTTTAATTTTATTTTTTTCGTAGAGTTTTTTTTTTTTTTTGTAAATCGCGTCATCAGATTCAAATTCAAATCCTGTTACAAGGCTCATAATCTGTATATTAATTGAGAGAGATTAAGTAGTAACTTTGATAATTTTTCAGGTCCGGGTTGTTCTTCCGTTGGAGGAGGTGCCTTTACTGAATTGGGTCCATTTTATCCTACTGGTGATGGACGTGGTTTACGAAAGAATTCAATGTCCTGGAATAGAGGTATCAATTTTGTTTATTATGGTTTGAGTTTGTGTTTTGATTGATATTAGAGAATAATTTGGGTTTATATATAGATTCCTGATTTTAATATTGTGTTTACTTTTTTTTATCATAGCTTCCAATCTTCTCTTTATTGAGTCTCCTGCTGGAGTTGGTTGGTCATACTCAAATACAACTTCCGATTACAACATCGGTGACGAGGCCACAGGTACTGATTTAAACTTTTCTGTGAACAAATTTATGTAGTTATTATTATCTATGCAAAAGAAAACATTATTTTTAGATGTGTTATGTATGAAATTTGTTAGTTTTATAATGCATGGATATAATCCTTTAATTTGTTTTTGGCAGCCAATGATATGCTTTCGTTCTTCCTGAAATGGTTCGAGAAGTTTCCTACCTACAAATCCAGAGCGTTGTTTCTTACCGGAGAAAGCTATGCAGGTCCAAAAGACACATTAATCAGATCCCAATTTTAATTATTAATTTATCGTTTCGAAATTCTCAAAAGTTGGTCTCGTGTTACAAAATATTCTGTTTTGCTGACATAATTCAGTTATACTGCATAATTAAACCTCGTATTGTATTCTTTATGCATATGCACATAACCTATGCCATCATTGCTAACTGGGATATTTGCTATACAATTATTATTTCATTTTATTTTTACATTGTTTATTTAAATATTTGTTCCTCTTTTCTATTTTTATTATCTGTTTTCTTTTACCTTTGTTCTCATGAACGATTTTTGTTTCTTTACTAATACTTTATAATGTTTATTCAGGACATTACATTCCTCAGTTAGCTAATGCTATTCTTGATTTTAATGCTCATTCAACCGGTTACAAGTTCAATTTGAAAGGAGTAGCTGTAAGATCCCATTCATATGATATCTTATATTTGGCTATTGAGTGTTTAATTAAGAAATCTGGGAATTTAATTAAAGTAATCTAAGCTTTTTTCTCTGGCTTTGTTAGATTGGCAACCCACTTTTGAATCTGGATCGAGATGCACGAGCACCATATGATTATTTCTGGTCACATGGAATGATTTCAGATGAAATTGGCCTTGCCATTACAAAAGACTGCGATTTTGATGATTATACCTTCGCATCTCCTCATAATGTATCTGCATCATGCAACACTGCCATCAGTGATGCAAATGAAGTTGTTGGCAATTATATAAACAACTATGATGTGATTCTTGATGTTTGTTATCCATCCATAGTTGAACAAGAATTGAGATTGAAAAAGATGGTACTTTTTTGCCTCTTTCATTTCTATTATACTACTAATGATATTTTTAACTTTTGATGAAATTATAAACTAATTAATTCGTCTATTTCGTTACTGCAGGCTACTAAAATAAGTATAGGTGTAGATGTCTGTATGAGTTATGAAAGAAAATTTTACTTCAACCTCCCTGAGGTTCAAAAGGCTCTCCATGCGAACCGTACCAACTTGCCGTATTCATGGTCCATGTGCAGTGGGTAAGTATAAATTAGAGACAGTCAAGTCCCATAAACTATTATTAGCGACACTCAAGTCCCATAAACTAGAGATATCGTCTTTTAATATAGCTTCTAAAATATAAACAATCATTGCCTTACATGTTGATTTTTTTCGATTGAGTTTTGCTATTTGAGATTTTTAAACTTACTTGAAATCTTGACTGCTATAATCATAATAGATATGTCTTTTTTTTTTTTTTTTTTTTTTTTCATTTTCATTAGTGATCTGATGTCTGATCTGCAGTTTTTCTTCATTGCAGTGTTTTAAATTACAGTGATACAGATCCTAACATTAATATGCTTCCAATTCTCAAACGGATTGTTCAAAACCGTATTCCAGTATGGGTTTTCAGGTAATTAATTTCAACAAAAGAGACCAATTGGCTATTTAATTTATACATTTTGATAGTCCCTATAAATACTATACTTTATCATTCATTTTACATCTTTTATCAGAACGACTCCTTACAAGTTTTACTCACTCTCAGGCTTTCAAATCTCTATTGATCATTTCTCTTGAAATCCTCTGATTACTAAGAAGCTAATCATGTGTTAAATTATTATGACAGTGGAGACCAAGATTCCGTTGTGCCATTACTAGGTTCTCGAACGCTCATTCGTGAACTGGCTGATGACCTGAAGTTCAAGATTACAGTCCCATATGGAGCTTGGTTCCACAAAGGCCAGGTAATTATTTTTAACAGTTTAACCATTTTCTGCATTTCGAAAAATAAGTTCAGTATTCTATAGTATGGTTATGCAATTTTTTTCACTCCAGTTTTCCAAATCAACGGTGATTTTCGTCATTGTTGTCAAATAAAGGCTATAGTGACGCTATTGCGTAGTGGAATTTAAACAAATCATTATTGTTATGAGATACGCTATTTAGCGGCGCTACAACATTGTAGTTTTGCGGAATTAACAAACCATTATTTTCTACAATTCTTGGTTGACAACACTAGTTTTGATGAGCAAGAGTTGATTGATTGCATATCTATGTGTAGGTTGGAGGTTGGGCAACTGAGTATGGAAATTTGTTAACTTTCGCCACCGTGCGGGGTGCATCTCACATGGTACCATATGCACAACCTTCTAGAGCACTGCATCTGTTCAGTAACTTTGTGAATGGTAGGAGATTGCCAAACACAACAAGGCCTTCAATTGATGGATGA
Protein sequence

>MS.gene50212.t1

MAQYWFLSVLIAVSYLCNLLVEGYPIEDLVVSLPGQPKVEFSQYAGYVDIDVKHGRSLFYYFVEADHKPQNKPLTLWLNGGPGCSSVGGGAFTELGPFYPTGDGRGLRKNSMSWNRASNLLFIESPAGVGWSYSNTTSDYNIGDEATANDMLSFFLKWFEKFPTYKSRALFLTGESYAGHYIPQLANAILDFNAHSTGYKFNLKGVAIGNPLLNLDRDARAPYDYFWSHGMISDEIGLAITKDCDFDDYTFASPHNVSASCNTAISDANEVVGNYINNYDVILDVCYPSIVEQELRLKKMATKISIGVDVCMSYERKFYFNLPEVQKALHANRTNLPYSWSMCSGVLNYSDTDPNINMLPILKRIVQNRIPVWVFSGDQDSVVPLLGSRTLIRELADDLKFKITVPYGAWFHKGQVGGWATEYGNLLTFATVRGASHMVPYAQPSRALHLFSNFVNGRRLPNTTRPSIDG