Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50568.t1 | XP_004500230.1 | 55.6 | 72 | 7 | 1 | 4 | 50 | 128 | 199 | 1.30E-09 | 72 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50568.t1 | A0A1S2Y6M5 | 55.6 | 72 | 7 | 1 | 4 | 50 | 128 | 199 | 9.5e-10 | 72.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056174 | MS.gene50568 | 0.803802 | 2.93E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50568.t1 | MTR_3g462790 | 54.167 | 72 | 8 | 1 | 4 | 50 | 117 | 188 | 6.42e-17 | 72.8 |
| MS.gene50568.t1 | MTR_5g069710 | 45.833 | 72 | 13 | 2 | 4 | 50 | 103 | 173 | 7.18e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTCTTCCTTTCCTGATC+AGG | 0.329243 | 1.2:-54263640 | None:intergenic |
| GTGAGTTCTTCTGTTTATAA+TGG | 0.342042 | 1.2:+54263574 | MS.gene50568:CDS |
| ACTGCAAAGAGAGGCTCTTT+TGG | 0.360910 | 1.2:+54263610 | MS.gene50568:CDS |
| CTATGTTTCTCTTCGGTCCA+TGG | 0.450029 | 1.2:-54263667 | None:intergenic |
| TTTCTTGCAGTAATTATGAC+GGG | 0.452089 | 1.2:+54264404 | MS.gene50568:intron |
| TCCATACCTATGTTTCTCTT+CGG | 0.456234 | 1.2:-54263674 | None:intergenic |
| AAAATTGAATTTGAAGGCGG+TGG | 0.501288 | 1.2:+54264066 | MS.gene50568:CDS |
| CCTGATCAGGAAAGGAAGAA+AGG | 0.540469 | 1.2:+54263640 | MS.gene50568:CDS |
| AGAGGAATTACTGCAAAGAG+AGG | 0.554018 | 1.2:+54263601 | MS.gene50568:CDS |
| AAAGGAAGAAAGGTGTTCCA+TGG | 0.554656 | 1.2:+54263650 | MS.gene50568:CDS |
| TGCAGTAATTATGACGGGGT+TGG | 0.556333 | 1.2:+54264409 | MS.gene50568:intron |
| TCATGGCTAGATACTTGTGT+TGG | 0.563631 | 1.2:-54264028 | None:intergenic |
| CATGGACCGAAGAGAAACAT+AGG | 0.594700 | 1.2:+54263668 | MS.gene50568:CDS |
| GTAGATCTCCTGATCAGGAA+AGG | 0.602196 | 1.2:+54263632 | MS.gene50568:CDS |
| TTCTTGCAGTAATTATGACG+GGG | 0.666715 | 1.2:+54264405 | MS.gene50568:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAAAAAATTGAATTTGA+AGG | + | chr1.2:54264060-54264079 | MS.gene50568:CDS | 15.0% |
| !!! | ATATTTATTTAAGTTGTTGA+GGG | + | chr1.2:54263883-54263902 | MS.gene50568:intron | 15.0% |
| !!! | GGAATTTAAATTTTTAAGAT+AGG | - | chr1.2:54264131-54264150 | None:intergenic | 15.0% |
| !!! | TTGTTATTGATTGATATATA+CGG | - | chr1.2:54264353-54264372 | None:intergenic | 15.0% |
| !! | TAAAAAATTGAATTTGAAGG+CGG | + | chr1.2:54264063-54264082 | MS.gene50568:CDS | 20.0% |
| !! | TGATGAATCATATAAGAAAT+TGG | + | chr1.2:54263468-54263487 | MS.gene50568:intron | 20.0% |
| !! | TTTACCCTTTACTTTATATT+AGG | + | chr1.2:54263552-54263571 | MS.gene50568:intron | 20.0% |
| !!! | ACAACTTTACTATTTTTGTA+TGG | + | chr1.2:54264167-54264186 | MS.gene50568:intron | 20.0% |
| !!! | ATTGACATGTTATTTCTATT+GGG | + | chr1.2:54263941-54263960 | MS.gene50568:intron | 20.0% |
| !!! | GATATTTATTTAAGTTGTTG+AGG | + | chr1.2:54263882-54263901 | MS.gene50568:intron | 20.0% |
| !!! | TATTTATTTAAGTTGTTGAG+GGG | + | chr1.2:54263884-54263903 | MS.gene50568:intron | 20.0% |
| ! | AATATACATGATAGCTTAGT+AGG | - | chr1.2:54264198-54264217 | None:intergenic | 25.0% |
| ! | ACTCACCTAATATAAAGTAA+AGG | - | chr1.2:54263560-54263579 | None:intergenic | 25.0% |
| ! | ATATTGTAGTAAGATGAGTA+TGG | + | chr1.2:54264242-54264261 | MS.gene50568:intron | 25.0% |
| ! | CTCACCTAATATAAAGTAAA+GGG | - | chr1.2:54263559-54263578 | None:intergenic | 25.0% |
| ! | TCATCTTACTACAATATCAT+TGG | - | chr1.2:54264239-54264258 | None:intergenic | 25.0% |
| !! | CATTGGTAATAGATGATAAA+TGG | - | chr1.2:54264222-54264241 | None:intergenic | 25.0% |
| !! | TTGACTTAACTTCATTTTAG+TGG | + | chr1.2:54264309-54264328 | MS.gene50568:intron | 25.0% |
| !! | TTTTCTTGCAGTAATTATGA+CGG | + | chr1.2:54264403-54264422 | MS.gene50568:intron | 25.0% |
| !!! | CATTGACATGTTATTTCTAT+TGG | + | chr1.2:54263940-54263959 | MS.gene50568:intron | 25.0% |
| !!! | GAAGTTGATGTTTGTAATAT+AGG | + | chr1.2:54263490-54263509 | MS.gene50568:intron | 25.0% |
| !!! | GCTGGTTTAGTTATAATTTT+TGG | + | chr1.2:54263514-54263533 | MS.gene50568:intron | 25.0% |
| !!! | TCTGTTTATAATGGTTTTAG+AGG | + | chr1.2:54263583-54263602 | MS.gene50568:CDS | 25.0% |
| !!! | TGACTTAACTTCATTTTAGT+GGG | + | chr1.2:54264310-54264329 | MS.gene50568:intron | 25.0% |
| !!! | TGGTCTTTTACATTTTGATA+TGG | + | chr1.2:54263814-54263833 | MS.gene50568:intron | 25.0% |
| AAAAGGATGGAAAAGGTAAT+TGG | + | chr1.2:54263969-54263988 | MS.gene50568:intron | 30.0% | |
| ATACGTAGTTATTGAGAGTT+AGG | + | chr1.2:54263773-54263792 | MS.gene50568:intron | 30.0% | |
| ATTTCTATTGGGTCTGAAAA+AGG | + | chr1.2:54263952-54263971 | MS.gene50568:intron | 30.0% | |
| GTATCATTTCAGATGCTTTA+TGG | - | chr1.2:54263427-54263446 | None:intergenic | 30.0% | |
| GTGAGTTCTTCTGTTTATAA+TGG | + | chr1.2:54263574-54263593 | MS.gene50568:CDS | 30.0% | |
| TGATCACAAAATTCCTTGAA+AGG | - | chr1.2:54264000-54264019 | None:intergenic | 30.0% | |
| TTTCTTGCAGTAATTATGAC+GGG | + | chr1.2:54264404-54264423 | MS.gene50568:intron | 30.0% | |
| ! | AAAGCATCTGAAATGATACA+TGG | + | chr1.2:54263428-54263447 | MS.gene50568:intron | 30.0% |
| ! | AGTAAAGTTGTCTTCTAGAA+AGG | - | chr1.2:54264158-54264177 | None:intergenic | 30.0% |
| ! | CAAGGAATTTTGTGATCATT+AGG | + | chr1.2:54264002-54264021 | MS.gene50568:intron | 30.0% |
| !! | TTTTACTTGTTGAAGCTTCA+TGG | - | chr1.2:54264048-54264067 | None:intergenic | 30.0% |
| AAAATTGAATTTGAAGGCGG+TGG | + | chr1.2:54264066-54264085 | MS.gene50568:CDS | 35.0% | |
| AACTCAAAATCTCCAGTCTT+AGG | + | chr1.2:54264266-54264285 | MS.gene50568:intron | 35.0% | |
| GAGAGTTAGGTATTTCCTAA+TGG | + | chr1.2:54263786-54263805 | MS.gene50568:intron | 35.0% | |
| GATGTTTGTAATATAGGAGC+TGG | + | chr1.2:54263496-54263515 | MS.gene50568:intron | 35.0% | |
| GTAATTGGAGAAACCTTTCA+AGG | + | chr1.2:54263984-54264003 | MS.gene50568:intron | 35.0% | |
| GTGTTGTCCTTATGCTTAAA+AGG | + | chr1.2:54264100-54264119 | MS.gene50568:intron | 35.0% | |
| TAAAAGACCACAACACCATT+AGG | - | chr1.2:54263804-54263823 | None:intergenic | 35.0% | |
| TCCATACCTATGTTTCTCTT+CGG | - | chr1.2:54263677-54263696 | None:intergenic | 35.0% | |
| TTCTTGCAGTAATTATGACG+GGG | + | chr1.2:54264405-54264424 | MS.gene50568:intron | 35.0% | |
| TTGTCCTTATGCTTAAAAGG+TGG | + | chr1.2:54264103-54264122 | MS.gene50568:intron | 35.0% | |
| ! | GTTGTCTTCTAGAAAGGAAA+AGG | - | chr1.2:54264152-54264171 | None:intergenic | 35.0% |
| ACCGAAGAGAAACATAGGTA+TGG | + | chr1.2:54263673-54263692 | MS.gene50568:intron | 40.0% | |
| AGAGGAATTACTGCAAAGAG+AGG | + | chr1.2:54263601-54263620 | MS.gene50568:CDS | 40.0% | |
| CAAAATCTCCAGTCTTAGGA+GGG | + | chr1.2:54264270-54264289 | MS.gene50568:intron | 40.0% | |
| CTATTGGGTCTGAAAAAGGA+TGG | + | chr1.2:54263956-54263975 | MS.gene50568:intron | 40.0% | |
| GCATAAGGACAACACTTACA+TGG | - | chr1.2:54264095-54264114 | None:intergenic | 40.0% | |
| GGTCTGAAAAAGGATGGAAA+AGG | + | chr1.2:54263962-54263981 | MS.gene50568:intron | 40.0% | |
| TCAAAATCTCCAGTCTTAGG+AGG | + | chr1.2:54264269-54264288 | MS.gene50568:intron | 40.0% | |
| TCATGGCTAGATACTTGTGT+TGG | - | chr1.2:54264031-54264050 | None:intergenic | 40.0% | |
| ! | GGAACCACCTTTTAAGCATA+AGG | - | chr1.2:54264110-54264129 | None:intergenic | 40.0% |
| ! | GGTATTTCCTAATGGTGTTG+TGG | + | chr1.2:54263794-54263813 | MS.gene50568:intron | 40.0% |
| ! | TATTTTGTCCCTCCTAAGAC+TGG | - | chr1.2:54264281-54264300 | None:intergenic | 40.0% |
| ! | TTTTGGTAGATCTCCTGATC+AGG | + | chr1.2:54263627-54263646 | MS.gene50568:CDS | 40.0% |
| !! | AAAGGAAGAAAGGTGTTCCA+TGG | + | chr1.2:54263650-54263669 | MS.gene50568:CDS | 40.0% |
| CATGGACCGAAGAGAAACAT+AGG | + | chr1.2:54263668-54263687 | MS.gene50568:CDS | 45.0% | |
| CCTGATCAGGAAAGGAAGAA+AGG | + | chr1.2:54263640-54263659 | MS.gene50568:CDS | 45.0% | |
| CCTTTCTTCCTTTCCTGATC+AGG | - | chr1.2:54263643-54263662 | None:intergenic | 45.0% | |
| CTATGTTTCTCTTCGGTCCA+TGG | - | chr1.2:54263670-54263689 | None:intergenic | 45.0% | |
| GTAGATCTCCTGATCAGGAA+AGG | + | chr1.2:54263632-54263651 | MS.gene50568:CDS | 45.0% | |
| ! | ACTGCAAAGAGAGGCTCTTT+TGG | + | chr1.2:54263610-54263629 | MS.gene50568:CDS | 45.0% |
| ! | TGCAGTAATTATGACGGGGT+TGG | + | chr1.2:54264409-54264428 | MS.gene50568:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 54263367 | 54264444 | 54263367 | ID=MS.gene50568 |
| chr1.2 | mRNA | 54263367 | 54264444 | 54263367 | ID=MS.gene50568.t1;Parent=MS.gene50568 |
| chr1.2 | exon | 54263367 | 54263375 | 54263367 | ID=MS.gene50568.t1.exon1;Parent=MS.gene50568.t1 |
| chr1.2 | CDS | 54263367 | 54263375 | 54263367 | ID=cds.MS.gene50568.t1;Parent=MS.gene50568.t1 |
| chr1.2 | exon | 54263574 | 54263689 | 54263574 | ID=MS.gene50568.t1.exon2;Parent=MS.gene50568.t1 |
| chr1.2 | CDS | 54263574 | 54263689 | 54263574 | ID=cds.MS.gene50568.t1;Parent=MS.gene50568.t1 |
| chr1.2 | exon | 54264024 | 54264095 | 54264024 | ID=MS.gene50568.t1.exon3;Parent=MS.gene50568.t1 |
| chr1.2 | CDS | 54264024 | 54264095 | 54264024 | ID=cds.MS.gene50568.t1;Parent=MS.gene50568.t1 |
| chr1.2 | exon | 54264414 | 54264444 | 54264414 | ID=MS.gene50568.t1.exon4;Parent=MS.gene50568.t1 |
| chr1.2 | CDS | 54264414 | 54264444 | 54264414 | ID=cds.MS.gene50568.t1;Parent=MS.gene50568.t1 |
| Gene Sequence |
| Protein sequence |
