AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50697

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50697.t1 MTR_4g072110 98.243 569 8 2 1 567 1 569 0.0 1135
MS.gene50697.t1 MTR_3g117590 77.475 404 60 2 128 531 27 399 0.0 630
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50697.t1 AT4G34740 81.944 504 81 2 51 553 67 561 0.0 847
MS.gene50697.t1 AT2G16570 83.198 494 77 3 62 553 77 566 0.0 845
MS.gene50697.t1 AT4G38880 68.952 525 133 7 6 526 27 525 0.0 716

Find 143 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGATAGCTGTTCCTGATTC+TGG 0.178498 4.3:+33707416 MS.gene50697:CDS
GGATGAGTGTTGAAGAAATA+AGG 0.235614 4.3:+33707780 MS.gene50697:CDS
GGGTTTAGGCCTTTGGTTAT+GGG 0.237862 4.3:+33707110 MS.gene50697:CDS
TCAACAATTCTTAAAATAAA+AGG 0.259457 4.3:-33707008 None:intergenic
TGGGTTTAGGCCTTTGGTTA+TGG 0.266896 4.3:+33707109 MS.gene50697:CDS
GAGAGGATGATGTCGGAAAT+TGG 0.268968 4.3:-33706504 None:intergenic
ATTATTGGTTCTTGTTACTA+TGG 0.269970 4.3:+33707719 MS.gene50697:CDS
ATTGCAAGTCCACCAATTAT+TGG 0.272119 4.3:+33707704 MS.gene50697:CDS
TGACGATGCCGGCGCCTTCT+TGG 0.275304 4.3:-33706668 None:intergenic
GTGCTGTGCTTGAAGGTAAA+AGG 0.278979 4.3:+33707585 MS.gene50697:CDS
GTTATTGTGGTTGATAATAA+TGG 0.288801 4.3:+33707227 MS.gene50697:CDS
GTAGCAGTATTCACCTCTTT+TGG 0.290374 4.3:-33708022 None:intergenic
TGAGGGATCCGTTTGGGTTT+AGG 0.298862 4.3:+33707096 MS.gene50697:CDS
GGGAGGGAGAAGGGAGGGTT+TGG 0.307776 4.3:-33706385 None:intergenic
TCCGTTTGGGTTTAGGCCTT+TGG 0.309909 4.3:+33707103 MS.gene50697:CDS
AGAGCTAAGCTTGAAGATAA+AGG 0.329947 4.3:+33706918 MS.gene50697:CDS
TGGCCGATGGCTAAGCTTCC+AGG 0.331879 4.3:-33706774 None:intergenic
CTCTTAACCTTGAGCTCTCT+TGG 0.333802 4.3:-33707920 None:intergenic
TATGAATCGCGTAGGCGATT+TGG 0.338464 4.3:+33707353 MS.gene50697:CDS
TGATGGTTTGAATGGGAGTT+TGG 0.341680 4.3:+33707961 MS.gene50697:CDS
TTGAGCCGTCTCAGAAGATT+AGG 0.349524 4.3:+33707528 MS.gene50697:CDS
TTATTGTGGTTGATAATAAT+GGG 0.351879 4.3:+33707228 MS.gene50697:CDS
TTGAGCTCTCTTGGTTCAAC+AGG 0.352244 4.3:-33707911 None:intergenic
AGAGGATGATGTCGGAAATT+GGG 0.360260 4.3:-33706503 None:intergenic
CGCCTTCTTGGCCGCGGTGT+TGG 0.365653 4.3:-33706656 None:intergenic
TTTGTTGCTGGGTACCGTTT+TGG 0.372205 4.3:+33706846 MS.gene50697:CDS
CCGGTCCGTGCTGTGCTTGA+AGG 0.375757 4.3:+33707578 MS.gene50697:CDS
GTTGGTGACTTTGTTGATGA+TGG 0.383115 4.3:+33707944 MS.gene50697:CDS
TAGTAACAAGAACCAATAAT+TGG 0.384192 4.3:-33707716 None:intergenic
CAGAGGAGTTGATATCTAAT+AGG 0.384260 4.3:+33707759 MS.gene50697:CDS
CTAATCAACCCTTGCTGAAA+AGG 0.384647 4.3:-33707479 None:intergenic
CGTGAAGAGTGCGGAGTTGT+TGG 0.401638 4.3:+33706579 MS.gene50697:CDS
GAAGATTGTGAGGTTGCTTA+AGG 0.403149 4.3:+33707652 MS.gene50697:CDS
GGAGAAGGGAGGGTTTGGAG+AGG 0.406436 4.3:-33706380 None:intergenic
TTTGTTGATGATGGTTTGAA+TGG 0.415105 4.3:+33707953 MS.gene50697:CDS
ATTGTTGTTGGGAGGGAGAA+GGG 0.420336 4.3:-33706394 None:intergenic
CAAGAGAGCTCAAGGTTAAG+AGG 0.423035 4.3:+33707921 MS.gene50697:CDS
GTCGGAGAGAGAAGGGAGAG+TGG 0.430326 4.3:-33706445 None:intergenic
ATACTATGCAAGCTATTGAT+TGG 0.430909 4.3:-33707836 None:intergenic
GATGAGTGTTGAAGAAATAA+GGG 0.433460 4.3:+33707781 MS.gene50697:CDS
GCAGCCACAACACCAGAATC+AGG 0.436019 4.3:-33707428 None:intergenic
TTGCTGTGAGGGATCCGTTT+GGG 0.437418 4.3:+33707090 MS.gene50697:CDS
TTTGGAGGAAGTGAGGAAAA+GGG 0.443047 4.3:-33706478 None:intergenic
GGAACAAATTGATGGTGGTT+GGG 0.451957 4.3:+33707982 MS.gene50697:CDS
AATAGCTTGCATAGTATGTT+GGG 0.456007 4.3:+33707842 MS.gene50697:CDS
TCTGGTGTTGTGGCTGCGCT+TGG 0.458740 4.3:+33707434 MS.gene50697:CDS
GAGAAGGGAGGGTTTGGAGA+GGG 0.461786 4.3:-33706379 None:intergenic
CAATAGCTTGCATAGTATGT+TGG 0.465848 4.3:+33707841 MS.gene50697:CDS
ATCACAACATCACAATCAAC+AGG 0.467162 4.3:-33707398 None:intergenic
GATTGTTGTTGGGAGGGAGA+AGG 0.470186 4.3:-33706395 None:intergenic
TTTGTTATGCTTGTTTCTCT+GGG 0.472388 4.3:+33707882 MS.gene50697:CDS
CTTGGTTATGCTGCGAAAGC+CGG 0.474447 4.3:+33707452 MS.gene50697:CDS
TTCTATTGTGTTTGTTACTG+AGG 0.480525 4.3:+33707058 MS.gene50697:CDS
TGCGGAGTTGTTGGAATCTA+CGG 0.482381 4.3:+33706588 MS.gene50697:CDS
GGGAGTTTGGAACAAATTGA+TGG 0.485386 4.3:+33707974 MS.gene50697:CDS
CATGTTCGTTATTCTACTGC+TGG 0.487415 4.3:+33706795 MS.gene50697:CDS
GTTGCTGTGAGGGATCCGTT+TGG 0.488507 4.3:+33707089 MS.gene50697:CDS
GTCGAAGAAGCGATTGTTGT+TGG 0.490307 4.3:-33706406 None:intergenic
TTGTTGGGAGGGAGAAGGGA+GGG 0.490395 4.3:-33706390 None:intergenic
TGCCGGCGCCTTCTTGGCCG+CGG 0.494734 4.3:-33706662 None:intergenic
ACAAAACTTGATCAGTTACC+TGG 0.496097 4.3:+33706756 MS.gene50697:CDS
CTCCAACACCGCGGCCAAGA+AGG 0.497456 4.3:+33706654 MS.gene50697:CDS
GGTTTAGGCCTTTGGTTATG+GGG 0.500914 4.3:+33707111 MS.gene50697:CDS
TGAGCCGTCTCAGAAGATTA+GGG 0.501845 4.3:+33707529 MS.gene50697:CDS
AGGGAGAGTGGATTTAGAGA+GGG 0.502352 4.3:-33706433 None:intergenic
AAGGGAGAGTGGATTTAGAG+AGG 0.504650 4.3:-33706434 None:intergenic
GACAAGACCAACACCGGTGA+TGG 0.504668 4.3:-33706715 None:intergenic
TGCTCAAAGATACACTGCTT+CGG 0.506723 4.3:-33707284 None:intergenic
TGGAACAAATTGATGGTGGT+TGG 0.506884 4.3:+33707981 MS.gene50697:CDS
GAGCTCAAGGTTAAGAGGGT+TGG 0.509152 4.3:+33707926 MS.gene50697:CDS
TCGAAGAAGCGATTGTTGTT+GGG 0.511241 4.3:-33706405 None:intergenic
TGGAAGAAAAGCAAGTGAAT+CGG 0.512363 4.3:-33707816 None:intergenic
AAAGAGGTGAATACTGCTAC+TGG 0.516631 4.3:+33708025 MS.gene50697:CDS
TTGGAGGAAGTGAGGAAAAG+GGG 0.517107 4.3:-33706477 None:intergenic
GTGAGGTTGCTTAAGGAAGC+TGG 0.520822 4.3:+33707659 MS.gene50697:CDS
TTTCGGGTGTCGGAGAGAGA+AGG 0.521313 4.3:-33706453 None:intergenic
AAACGGTACCCAGCAACAAA+AGG 0.521430 4.3:-33706843 None:intergenic
TTTGTTTGAGAGGATGATGT+CGG 0.523646 4.3:-33706511 None:intergenic
CTTCAATCCATCACCGGTGT+TGG 0.525788 4.3:+33706708 MS.gene50697:CDS
TCTCAGAAGATTAGGGACTT+TGG 0.526450 4.3:+33707536 MS.gene50697:CDS
TTGTTGATGATGGTTTGAAT+GGG 0.530340 4.3:+33707954 MS.gene50697:CDS
GTTGTTGACGGTGACGATGC+CGG 0.533028 4.3:-33706679 None:intergenic
GGAGGGCTAAGTAGCAGAGA+CGG 0.535500 4.3:-33706626 None:intergenic
TGTTCCTGATTCTGGTGTTG+TGG 0.536036 4.3:+33707424 MS.gene50697:CDS
TGGTTCTTGTTACTATGGTG+TGG 0.539936 4.3:+33707724 MS.gene50697:CDS
GATATCAACTCCTCTGAGCT+AGG 0.540111 4.3:-33707752 None:intergenic
AAGATACACTGCTTCGGCTG+CGG 0.540981 4.3:-33707278 None:intergenic
TCAACCACAATAACCTCACC+AGG 0.542973 4.3:-33707218 None:intergenic
GAGGGCTAAGTAGCAGAGAC+GGG 0.544400 4.3:-33706625 None:intergenic
TTGAAGCACGTTGTTGTTGA+CGG 0.547527 4.3:-33706691 None:intergenic
TTTCCCTGGTGAGGTTATTG+TGG 0.547630 4.3:+33707214 MS.gene50697:CDS
CCCTTGCTGAAAAGGAACAC+CGG 0.547689 4.3:-33707471 None:intergenic
CACCGCGGCCAAGAAGGCGC+CGG 0.548864 4.3:+33706660 MS.gene50697:CDS
GGATAAGCTTGTTGCTGTGA+GGG 0.549006 4.3:+33707079 MS.gene50697:CDS
GGCCGCGGTGTTGGAGAGCG+TGG 0.550054 4.3:-33706647 None:intergenic
CGTTGATGATTCAATTGTGA+GGG 0.550589 4.3:+33707616 MS.gene50697:CDS
GTTGTTGGGAGGGAGAAGGG+AGG 0.552388 4.3:-33706391 None:intergenic
ACCAAAGGCCTAAACCCAAA+CGG 0.556825 4.3:-33707104 None:intergenic
TGCTGTGCTTGAAGGTAAAA+GGG 0.558567 4.3:+33707586 MS.gene50697:CDS
TAACAAGAACCAATAATTGG+TGG 0.560555 4.3:-33707713 None:intergenic
GCGGTGTTGGAGAGCGTGGA+GGG 0.560878 4.3:-33706643 None:intergenic
AGCCAACAGAAATCCAAAAG+AGG 0.563771 4.3:+33708009 MS.gene50697:CDS
TTCGGGTGTCGGAGAGAGAA+GGG 0.564516 4.3:-33706452 None:intergenic
TTAGGCCTTTGGTTATGGGG+AGG 0.567193 4.3:+33707114 MS.gene50697:CDS
GATGCATGTGAGAAGCTTGA+AGG 0.569398 4.3:+33707029 MS.gene50697:CDS
CAACACCCACAGAACCAAAA+CGG 0.569611 4.3:-33706860 None:intergenic
TTGATTAGGTCACACTATGT+AGG 0.570960 4.3:+33707494 MS.gene50697:CDS
CCTTCAAGCACAGCACGGAC+CGG 0.573470 4.3:-33707578 None:intergenic
TGTGAAGTTGAAGCTTTCAC+CGG 0.573769 4.3:+33707559 MS.gene50697:CDS
AAGAGAGCTCAAGGTTAAGA+GGG 0.575571 4.3:+33707922 MS.gene50697:CDS
GATTGAAGCTACTTATGAGA+GGG 0.584297 4.3:+33707187 MS.gene50697:CDS
AGAAGCGATTGTTGTTGGGA+GGG 0.585596 4.3:-33706401 None:intergenic
AAGAAGCGATTGTTGTTGGG+AGG 0.590278 4.3:-33706402 None:intergenic
TGATTGAAGCTACTTATGAG+AGG 0.590354 4.3:+33707186 MS.gene50697:CDS
GTTATGGGGAGGAGAAGTAA+TGG 0.595209 4.3:+33707125 MS.gene50697:CDS
TGTTGAACCAAGAGAGCTCA+AGG 0.596313 4.3:+33707913 MS.gene50697:CDS
AAACATCCTCGTCTTGTGGG+TGG 0.601035 4.3:-33706536 None:intergenic
AACGTGCTTCAATCCATCAC+CGG 0.602027 4.3:+33706702 MS.gene50697:CDS
ATCGGAGACAAGACCAACAC+CGG 0.609069 4.3:-33706721 None:intergenic
CGCGGTGTTGGAGAGCGTGG+AGG 0.613758 4.3:-33706644 None:intergenic
TCGTTGATGATTCAATTGTG+AGG 0.613921 4.3:+33707615 MS.gene50697:CDS
ACAACTCCGCACTCTTCACG+AGG 0.619515 4.3:-33706576 None:intergenic
CAGCAGTAGAATAACGAACA+TGG 0.626295 4.3:-33706794 None:intergenic
TTACCTGGAAGCTTAGCCAT+CGG 0.629983 4.3:+33706771 MS.gene50697:CDS
AGGTTGAACATTCTTCAACA+TGG 0.631085 4.3:-33706823 None:intergenic
CAACATCGTCGAAGATTGTG+AGG 0.634583 4.3:+33707642 MS.gene50697:CDS
TCTGTGGGTGTTGCTCATAA+CGG 0.634744 4.3:+33706870 MS.gene50697:CDS
AGAAGAGAGAGTTTGAGCTG+CGG 0.634768 4.3:-33706355 None:intergenic
CTTCTCCTCCCCATAACCAA+AGG 0.635089 4.3:-33707119 None:intergenic
ATCGCGTAGGCGATTTGGTG+AGG 0.637082 4.3:+33707358 MS.gene50697:CDS
AAGTCCCTAATCTTCTGAGA+CGG 0.638630 4.3:-33707533 None:intergenic
TGAGCTCTCTTGGTTCAACA+GGG 0.647432 4.3:-33707910 None:intergenic
AGTTTGGAACAAATTGATGG+TGG 0.649376 4.3:+33707977 MS.gene50697:CDS
AGGATAAGCTTGTTGCTGTG+AGG 0.658984 4.3:+33707078 MS.gene50697:CDS
GGTCTGTTTATGAATCGCGT+AGG 0.659509 4.3:+33707345 MS.gene50697:CDS
AGAATAACGAACATGGCCGA+TGG 0.666671 4.3:-33706787 None:intergenic
GTGCAAAAGAAGTTCACATG+AGG 0.670165 4.3:+33707681 MS.gene50697:CDS
GACAAACCTCGTGAAGAGTG+CGG 0.677329 4.3:+33706570 MS.gene50697:CDS
GGCTAAGTAGCAGAGACGGG+AGG 0.683072 4.3:-33706622 None:intergenic
TGTGGATACACCTAGCTCAG+AGG 0.694828 4.3:+33707742 MS.gene50697:CDS
GTTGATGATTCAATTGTGAG+GGG 0.720824 4.3:+33707617 MS.gene50697:CDS
CAACCACAATAACCTCACCA+GGG 0.721289 4.3:-33707217 None:intergenic
CTCCACGCTCTCCAACACCG+CGG 0.815634 4.3:+33706645 MS.gene50697:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAACAATTCTTAAAATAAA+AGG - chr4.3:33707011-33707030 None:intergenic 15.0%
!! AAACAAGCATAACAAAAATT+AGG - chr4.3:33707878-33707897 None:intergenic 20.0%
!!! TTATTGTGGTTGATAATAAT+GGG + chr4.3:33707228-33707247 MS.gene50697:CDS 20.0%
!!! TTTTGTTTCATTGAATACAT+CGG - chr4.3:33706742-33706761 None:intergenic 20.0%
! TAGTAACAAGAACCAATAAT+TGG - chr4.3:33707719-33707738 None:intergenic 25.0%
!! GAAGAAATAAGGGATTTTAT+TGG + chr4.3:33707791-33707810 MS.gene50697:CDS 25.0%
!!! ATTATTGGTTCTTGTTACTA+TGG + chr4.3:33707719-33707738 MS.gene50697:CDS 25.0%
!!! GTTATTGTGGTTGATAATAA+TGG + chr4.3:33707227-33707246 MS.gene50697:CDS 25.0%
ATACTATGCAAGCTATTGAT+TGG - chr4.3:33707839-33707858 None:intergenic 30.0%
CTACCAAAAACAACAGAATT+AGG - chr4.3:33707326-33707345 None:intergenic 30.0%
TAACAAGAACCAATAATTGG+TGG - chr4.3:33707716-33707735 None:intergenic 30.0%
! AATAGCTTGCATAGTATGTT+GGG + chr4.3:33707842-33707861 MS.gene50697:CDS 30.0%
! TTCTATTGTGTTTGTTACTG+AGG + chr4.3:33707058-33707077 MS.gene50697:CDS 30.0%
! TTTGTTATGCTTGTTTCTCT+GGG + chr4.3:33707882-33707901 MS.gene50697:CDS 30.0%
!! GATGAGTGTTGAAGAAATAA+GGG + chr4.3:33707781-33707800 MS.gene50697:CDS 30.0%
!! TTGTTGATGATGGTTTGAAT+GGG + chr4.3:33707954-33707973 MS.gene50697:CDS 30.0%
!! TTTGTTGATGATGGTTTGAA+TGG + chr4.3:33707953-33707972 MS.gene50697:CDS 30.0%
!! TTTTGTTATGCTTGTTTCTC+TGG + chr4.3:33707881-33707900 MS.gene50697:CDS 30.0%
!!! CTAATTCTGTTGTTTTTGGT+AGG + chr4.3:33707324-33707343 MS.gene50697:CDS 30.0%
!!! TTGCCTAATTCTGTTGTTTT+TGG + chr4.3:33707320-33707339 MS.gene50697:CDS 30.0%
ACAAAACTTGATCAGTTACC+TGG + chr4.3:33706756-33706775 MS.gene50697:CDS 35.0%
AGAGCTAAGCTTGAAGATAA+AGG + chr4.3:33706918-33706937 MS.gene50697:CDS 35.0%
AGGTTGAACATTCTTCAACA+TGG - chr4.3:33706826-33706845 None:intergenic 35.0%
ATAAAAACATCCTCGTCTTG+TGG - chr4.3:33706543-33706562 None:intergenic 35.0%
ATCACAACATCACAATCAAC+AGG - chr4.3:33707401-33707420 None:intergenic 35.0%
ATTGCAAGTCCACCAATTAT+TGG + chr4.3:33707704-33707723 MS.gene50697:CDS 35.0%
GATTGAAGCTACTTATGAGA+GGG + chr4.3:33707187-33707206 MS.gene50697:CDS 35.0%
TAAAAACATCCTCGTCTTGT+GGG - chr4.3:33706542-33706561 None:intergenic 35.0%
TGATTGAAGCTACTTATGAG+AGG + chr4.3:33707186-33707205 MS.gene50697:CDS 35.0%
TGGAAGAAAAGCAAGTGAAT+CGG - chr4.3:33707819-33707838 None:intergenic 35.0%
TTGATTAGGTCACACTATGT+AGG + chr4.3:33707494-33707513 MS.gene50697:CDS 35.0%
TTTGTTTGAGAGGATGATGT+CGG - chr4.3:33706514-33706533 None:intergenic 35.0%
! AATGTTCAACCTTTTGTTGC+TGG + chr4.3:33706834-33706853 MS.gene50697:CDS 35.0%
! AGTTTGGAACAAATTGATGG+TGG + chr4.3:33707977-33707996 MS.gene50697:CDS 35.0%
! ATGTTCAACCTTTTGTTGCT+GGG + chr4.3:33706835-33706854 MS.gene50697:CDS 35.0%
! CAATAGCTTGCATAGTATGT+TGG + chr4.3:33707841-33707860 MS.gene50697:CDS 35.0%
!! CAGAGGAGTTGATATCTAAT+AGG + chr4.3:33707759-33707778 MS.gene50697:CDS 35.0%
!! CGTTGATGATTCAATTGTGA+GGG + chr4.3:33707616-33707635 MS.gene50697:CDS 35.0%
!! GGATGAGTGTTGAAGAAATA+AGG + chr4.3:33707780-33707799 MS.gene50697:CDS 35.0%
!! GTTGATGATTCAATTGTGAG+GGG + chr4.3:33707617-33707636 MS.gene50697:CDS 35.0%
!! TCGTTGATGATTCAATTGTG+AGG + chr4.3:33707615-33707634 MS.gene50697:CDS 35.0%
AAAGAGGTGAATACTGCTAC+TGG + chr4.3:33708025-33708044 MS.gene50697:CDS 40.0%
AAGAGAGCTCAAGGTTAAGA+GGG + chr4.3:33707922-33707941 MS.gene50697:CDS 40.0%
AGAGGATGATGTCGGAAATT+GGG - chr4.3:33706506-33706525 None:intergenic 40.0%
AGCCAACAGAAATCCAAAAG+AGG + chr4.3:33708009-33708028 MS.gene50697:CDS 40.0%
CAGCAGTAGAATAACGAACA+TGG - chr4.3:33706797-33706816 None:intergenic 40.0%
CATGTTCGTTATTCTACTGC+TGG + chr4.3:33706795-33706814 MS.gene50697:CDS 40.0%
CTAATCAACCCTTGCTGAAA+AGG - chr4.3:33707482-33707501 None:intergenic 40.0%
GAAGATTGTGAGGTTGCTTA+AGG + chr4.3:33707652-33707671 MS.gene50697:CDS 40.0%
GTGCAAAAGAAGTTCACATG+AGG + chr4.3:33707681-33707700 MS.gene50697:CDS 40.0%
TCTCAGAAGATTAGGGACTT+TGG + chr4.3:33707536-33707555 MS.gene50697:CDS 40.0%
TGATGGTTTGAATGGGAGTT+TGG + chr4.3:33707961-33707980 MS.gene50697:CDS 40.0%
TGCTCAAAGATACACTGCTT+CGG - chr4.3:33707287-33707306 None:intergenic 40.0%
TGCTGTGCTTGAAGGTAAAA+GGG + chr4.3:33707586-33707605 MS.gene50697:CDS 40.0%
TGGTTCTTGTTACTATGGTG+TGG + chr4.3:33707724-33707743 MS.gene50697:CDS 40.0%
TGTGAAGTTGAAGCTTTCAC+CGG + chr4.3:33707559-33707578 MS.gene50697:CDS 40.0%
TTTGGAGGAAGTGAGGAAAA+GGG - chr4.3:33706481-33706500 None:intergenic 40.0%
! AAGTCCCTAATCTTCTGAGA+CGG - chr4.3:33707536-33707555 None:intergenic 40.0%
! AAGTGAGGAAAAGGGGTTTT+CGG - chr4.3:33706473-33706492 None:intergenic 40.0%
! CACCTCTTTTGGATTTCTGT+TGG - chr4.3:33708014-33708033 None:intergenic 40.0%
! GATGTCGGAAATTGGGTTTT+TGG - chr4.3:33706499-33706518 None:intergenic 40.0%
! GGGAGTTTGGAACAAATTGA+TGG + chr4.3:33707974-33707993 MS.gene50697:CDS 40.0%
! GTAGCAGTATTCACCTCTTT+TGG - chr4.3:33708025-33708044 None:intergenic 40.0%
! TCGAAGAAGCGATTGTTGTT+GGG - chr4.3:33706408-33706427 None:intergenic 40.0%
! TTGAAGCACGTTGTTGTTGA+CGG - chr4.3:33706694-33706713 None:intergenic 40.0%
! TTTTACCTTCAAGCACAGCA+CGG - chr4.3:33707586-33707605 None:intergenic 40.0%
!! CTTTTCAGCAAGGGTTGATT+AGG + chr4.3:33707480-33707499 MS.gene50697:CDS 40.0%
!! GGAACAAATTGATGGTGGTT+GGG + chr4.3:33707982-33708001 MS.gene50697:CDS 40.0%
!! GTTGGTGACTTTGTTGATGA+TGG + chr4.3:33707944-33707963 MS.gene50697:CDS 40.0%
!! TGGAACAAATTGATGGTGGT+TGG + chr4.3:33707981-33708000 MS.gene50697:CDS 40.0%
!! TTTTGGAGGAAGTGAGGAAA+AGG - chr4.3:33706482-33706501 None:intergenic 40.0%
AAACGGTACCCAGCAACAAA+AGG - chr4.3:33706846-33706865 None:intergenic 45.0%
AACGTGCTTCAATCCATCAC+CGG + chr4.3:33706702-33706721 MS.gene50697:CDS 45.0%
ACCAAAGGCCTAAACCCAAA+CGG - chr4.3:33707107-33707126 None:intergenic 45.0%
AGAAGAGAGAGTTTGAGCTG+CGG - chr4.3:33706358-33706377 None:intergenic 45.0%
AGAATAACGAACATGGCCGA+TGG - chr4.3:33706790-33706809 None:intergenic 45.0%
CAACACCCACAGAACCAAAA+CGG - chr4.3:33706863-33706882 None:intergenic 45.0%
CAACATCGTCGAAGATTGTG+AGG + chr4.3:33707642-33707661 MS.gene50697:CDS 45.0%
CAACCACAATAACCTCACCA+GGG - chr4.3:33707220-33707239 None:intergenic 45.0%
CAAGAGAGCTCAAGGTTAAG+AGG + chr4.3:33707921-33707940 MS.gene50697:CDS 45.0%
CTCTTAACCTTGAGCTCTCT+TGG - chr4.3:33707923-33707942 None:intergenic 45.0%
GAGAGGATGATGTCGGAAAT+TGG - chr4.3:33706507-33706526 None:intergenic 45.0%
GATATCAACTCCTCTGAGCT+AGG - chr4.3:33707755-33707774 None:intergenic 45.0%
GATGCATGTGAGAAGCTTGA+AGG + chr4.3:33707029-33707048 MS.gene50697:CDS 45.0%
GGGTTTAGGCCTTTGGTTAT+GGG + chr4.3:33707110-33707129 MS.gene50697:CDS 45.0%
GGTCTGTTTATGAATCGCGT+AGG + chr4.3:33707345-33707364 MS.gene50697:CDS 45.0%
GGTTTAGGCCTTTGGTTATG+GGG + chr4.3:33707111-33707130 MS.gene50697:CDS 45.0%
GTCGAAGAAGCGATTGTTGT+TGG - chr4.3:33706409-33706428 None:intergenic 45.0%
GTGATAGCTGTTCCTGATTC+TGG + chr4.3:33707416-33707435 MS.gene50697:CDS 45.0%
GTGCTGTGCTTGAAGGTAAA+AGG + chr4.3:33707585-33707604 MS.gene50697:CDS 45.0%
GTTATGGGGAGGAGAAGTAA+TGG + chr4.3:33707125-33707144 MS.gene50697:CDS 45.0%
TATGAATCGCGTAGGCGATT+TGG + chr4.3:33707353-33707372 MS.gene50697:CDS 45.0%
TCAACCACAATAACCTCACC+AGG - chr4.3:33707221-33707240 None:intergenic 45.0%
TGAGCCGTCTCAGAAGATTA+GGG + chr4.3:33707529-33707548 MS.gene50697:CDS 45.0%
TGAGCTCTCTTGGTTCAACA+GGG - chr4.3:33707913-33707932 None:intergenic 45.0%
TGCGGAGTTGTTGGAATCTA+CGG + chr4.3:33706588-33706607 MS.gene50697:CDS 45.0%
TGGGTTTAGGCCTTTGGTTA+TGG + chr4.3:33707109-33707128 MS.gene50697:CDS 45.0%
TGTTGAACCAAGAGAGCTCA+AGG + chr4.3:33707913-33707932 MS.gene50697:CDS 45.0%
TTACCTGGAAGCTTAGCCAT+CGG + chr4.3:33706771-33706790 MS.gene50697:CDS 45.0%
TTGAGCCGTCTCAGAAGATT+AGG + chr4.3:33707528-33707547 MS.gene50697:CDS 45.0%
TTGAGCTCTCTTGGTTCAAC+AGG - chr4.3:33707914-33707933 None:intergenic 45.0%
TTGGAGGAAGTGAGGAAAAG+GGG - chr4.3:33706480-33706499 None:intergenic 45.0%
TTTCCCTGGTGAGGTTATTG+TGG + chr4.3:33707214-33707233 MS.gene50697:CDS 45.0%
! AAGAAGCGATTGTTGTTGGG+AGG - chr4.3:33706405-33706424 None:intergenic 45.0%
! AAGGGAGAGTGGATTTAGAG+AGG - chr4.3:33706437-33706456 None:intergenic 45.0%
! AGAAGCGATTGTTGTTGGGA+GGG - chr4.3:33706404-33706423 None:intergenic 45.0%
! AGGGAGAGTGGATTTAGAGA+GGG - chr4.3:33706436-33706455 None:intergenic 45.0%
! AGTGAGGAAAAGGGGTTTTC+GGG - chr4.3:33706472-33706491 None:intergenic 45.0%
! ATTGTTGTTGGGAGGGAGAA+GGG - chr4.3:33706397-33706416 None:intergenic 45.0%
! TATGAGAGGGAAGTTTTCCC+TGG + chr4.3:33707200-33707219 MS.gene50697:CDS 45.0%
! TGTTCCTGATTCTGGTGTTG+TGG + chr4.3:33707424-33707443 MS.gene50697:CDS 45.0%
!! AGGATAAGCTTGTTGCTGTG+AGG + chr4.3:33707078-33707097 MS.gene50697:CDS 45.0%
!! GGATAAGCTTGTTGCTGTGA+GGG + chr4.3:33707079-33707098 MS.gene50697:CDS 45.0%
!! GTCGGAAATTGGGTTTTTGG+AGG - chr4.3:33706496-33706515 None:intergenic 45.0%
!! GTGGGTGGTTTTTGTTTGAG+AGG - chr4.3:33706524-33706543 None:intergenic 45.0%
!! TCTGTGGGTGTTGCTCATAA+CGG + chr4.3:33706870-33706889 MS.gene50697:CDS 45.0%
!! TTGGGTTTTTGGAGGAAGTG+AGG - chr4.3:33706488-33706507 None:intergenic 45.0%
!! TTTGTTGCTGGGTACCGTTT+TGG + chr4.3:33706846-33706865 MS.gene50697:CDS 45.0%
AAACATCCTCGTCTTGTGGG+TGG - chr4.3:33706539-33706558 None:intergenic 50.0%
AAGATACACTGCTTCGGCTG+CGG - chr4.3:33707281-33707300 None:intergenic 50.0%
ATCGGAGACAAGACCAACAC+CGG - chr4.3:33706724-33706743 None:intergenic 50.0%
CAAAAACCACCCACAAGACG+AGG + chr4.3:33706530-33706549 MS.gene50697:CDS 50.0%
CCCTTGCTGAAAAGGAACAC+CGG - chr4.3:33707474-33707493 None:intergenic 50.0%
CTTCAATCCATCACCGGTGT+TGG + chr4.3:33706708-33706727 MS.gene50697:CDS 50.0%
CTTCTCCTCCCCATAACCAA+AGG - chr4.3:33707122-33707141 None:intergenic 50.0%
CTTGGTTATGCTGCGAAAGC+CGG + chr4.3:33707452-33707471 MS.gene50697:CDS 50.0%
GACAAACCTCGTGAAGAGTG+CGG + chr4.3:33706570-33706589 MS.gene50697:CDS 50.0%
GAGCTCAAGGTTAAGAGGGT+TGG + chr4.3:33707926-33707945 MS.gene50697:CDS 50.0%
GTGAGGTTGCTTAAGGAAGC+TGG + chr4.3:33707659-33707678 MS.gene50697:CDS 50.0%
TGTGGATACACCTAGCTCAG+AGG + chr4.3:33707742-33707761 MS.gene50697:CDS 50.0%
TTAGGCCTTTGGTTATGGGG+AGG + chr4.3:33707114-33707133 MS.gene50697:CDS 50.0%
TTGCTGTGAGGGATCCGTTT+GGG + chr4.3:33707090-33707109 MS.gene50697:CDS 50.0%
! CCGGTGTTCCTTTTCAGCAA+GGG + chr4.3:33707471-33707490 MS.gene50697:CDS 50.0%
! GATTGTTGTTGGGAGGGAGA+AGG - chr4.3:33706398-33706417 None:intergenic 50.0%
! TCCGTTTGGGTTTAGGCCTT+TGG + chr4.3:33707103-33707122 MS.gene50697:CDS 50.0%
! TGAGGGATCCGTTTGGGTTT+AGG + chr4.3:33707096-33707115 MS.gene50697:CDS 50.0%
!!! GAAAAGGGGTTTTCGGGTGT+CGG - chr4.3:33706466-33706485 None:intergenic 50.0%
!!! GGGTACCGTTTTGGTTCTGT+GGG + chr4.3:33706855-33706874 MS.gene50697:CDS 50.0%
!!! TGGGTACCGTTTTGGTTCTG+TGG + chr4.3:33706854-33706873 MS.gene50697:CDS 50.0%
ACAACTCCGCACTCTTCACG+AGG - chr4.3:33706579-33706598 None:intergenic 55.0%
GACAAGACCAACACCGGTGA+TGG - chr4.3:33706718-33706737 None:intergenic 55.0%
GAGAAGGGAGGGTTTGGAGA+GGG - chr4.3:33706382-33706401 None:intergenic 55.0%
GAGGGCTAAGTAGCAGAGAC+GGG - chr4.3:33706628-33706647 None:intergenic 55.0%
GCAGCCACAACACCAGAATC+AGG - chr4.3:33707431-33707450 None:intergenic 55.0%
GGAGGGCTAAGTAGCAGAGA+CGG - chr4.3:33706629-33706648 None:intergenic 55.0%
GTTGCTGTGAGGGATCCGTT+TGG + chr4.3:33707089-33707108 MS.gene50697:CDS 55.0%
GTTGTTGACGGTGACGATGC+CGG - chr4.3:33706682-33706701 None:intergenic 55.0%
TTGTTGGGAGGGAGAAGGGA+GGG - chr4.3:33706393-33706412 None:intergenic 55.0%
! CGTGAAGAGTGCGGAGTTGT+TGG + chr4.3:33706579-33706598 MS.gene50697:CDS 55.0%
! GAGGGAAGTTTTCCCTGGTG+AGG + chr4.3:33707205-33707224 MS.gene50697:CDS 55.0%
! GCCGGTGTTCCTTTTCAGCA+AGG + chr4.3:33707470-33707489 MS.gene50697:CDS 55.0%
!! ATCGCGTAGGCGATTTGGTG+AGG + chr4.3:33707358-33707377 MS.gene50697:CDS 55.0%
!! TTCGGGTGTCGGAGAGAGAA+GGG - chr4.3:33706455-33706474 None:intergenic 55.0%
!! TTTCGGGTGTCGGAGAGAGA+AGG - chr4.3:33706456-33706475 None:intergenic 55.0%
CCTTCAAGCACAGCACGGAC+CGG - chr4.3:33707581-33707600 None:intergenic 60.0%
GGAGAAGGGAGGGTTTGGAG+AGG - chr4.3:33706383-33706402 None:intergenic 60.0%
GGCTAAGTAGCAGAGACGGG+AGG - chr4.3:33706625-33706644 None:intergenic 60.0%
GTCGGAGAGAGAAGGGAGAG+TGG - chr4.3:33706448-33706467 None:intergenic 60.0%
GTTGTTGGGAGGGAGAAGGG+AGG - chr4.3:33706394-33706413 None:intergenic 60.0%
TGGCCGATGGCTAAGCTTCC+AGG - chr4.3:33706777-33706796 None:intergenic 60.0%
!! TCTGGTGTTGTGGCTGCGCT+TGG + chr4.3:33707434-33707453 MS.gene50697:CDS 60.0%
CCGGTCCGTGCTGTGCTTGA+AGG + chr4.3:33707578-33707597 MS.gene50697:CDS 65.0%
CTCCAACACCGCGGCCAAGA+AGG + chr4.3:33706654-33706673 MS.gene50697:CDS 65.0%
CTCCACGCTCTCCAACACCG+CGG + chr4.3:33706645-33706664 MS.gene50697:CDS 65.0%
GGGAGGGAGAAGGGAGGGTT+TGG - chr4.3:33706388-33706407 None:intergenic 65.0%
TGACGATGCCGGCGCCTTCT+TGG - chr4.3:33706671-33706690 None:intergenic 65.0%
! GCGGTGTTGGAGAGCGTGGA+GGG - chr4.3:33706646-33706665 None:intergenic 65.0%
CGCCTTCTTGGCCGCGGTGT+TGG - chr4.3:33706659-33706678 None:intergenic 70.0%
! CGCGGTGTTGGAGAGCGTGG+AGG - chr4.3:33706647-33706666 None:intergenic 70.0%
TGCCGGCGCCTTCTTGGCCG+CGG - chr4.3:33706665-33706684 None:intergenic 75.0%
! CACCGCGGCCAAGAAGGCGC+CGG + chr4.3:33706660-33706679 MS.gene50697:CDS 75.0%
! GGCCGCGGTGTTGGAGAGCG+TGG - chr4.3:33706650-33706669 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 33706351 33708054 33706351 ID=MS.gene50697
chr4.3 mRNA 33706351 33708054 33706351 ID=MS.gene50697.t1;Parent=MS.gene50697
chr4.3 exon 33706351 33708054 33706351 ID=MS.gene50697.t1.exon1;Parent=MS.gene50697.t1
chr4.3 CDS 33706351 33708054 33706351 ID=cds.MS.gene50697.t1;Parent=MS.gene50697.t1
Gene Sequence

>MS.gene50697

ATGGCCGCAGCTCAAACTCTCTCTTCTTCCCTCTCCAAACCCTCCCTTCTCCCTCCCAACAACAATCGCTTCTTCGACACATCCCTCTCTAAATCCACTCTCCCTTCTCTCTCCGACACCCGAAAACCCCTTTTCCTCACTTCCTCCAAAAACCCAATTTCCGACATCATCCTCTCAAACAAAAACCACCCACAAGACGAGGATGTTTTTATCAACGACGACAAACCTCGTGAAGAGTGCGGAGTTGTTGGAATCTACGGCGACTCAGAAGCCTCCCGTCTCTGCTACTTAGCCCTCCACGCTCTCCAACACCGCGGCCAAGAAGGCGCCGGCATCGTCACCGTCAACAACAACGTGCTTCAATCCATCACCGGTGTTGGTCTTGTCTCCGATGTATTCAATGAAACAAAACTTGATCAGTTACCTGGAAGCTTAGCCATCGGCCATGTTCGTTATTCTACTGCTGGACAGTCCATGTTGAAGAATGTTCAACCTTTTGTTGCTGGGTACCGTTTTGGTTCTGTGGGTGTTGCTCATAACGGTAATTTAGTTAATTATCGTACTTTAAGAGCTAAGCTTGAAGATAAAGGTTCGATTTTTAACACTACTTCTGATACTGAAGTTGTTCTTCATTTGATTGCTACTTCAAAGCATAGACCTTTTATTTTAAGAATTGTTGATGCATGTGAGAAGCTTGAAGGTGCTTATTCTATTGTGTTTGTTACTGAGGATAAGCTTGTTGCTGTGAGGGATCCGTTTGGGTTTAGGCCTTTGGTTATGGGGAGGAGAAGTAATGGTGCTGTTGTTTTTGCTTCTGAGACTTGTGCTCTTGATTTGATTGAAGCTACTTATGAGAGGGAAGTTTTCCCTGGTGAGGTTATTGTGGTTGATAATAATGGGATTCAATCGCTCTGTTTGATGTCGCATCCGCAGCCGAAGCAGTGTATCTTTGAGCATATTTACTTTGCTTTGCCTAATTCTGTTGTTTTTGGTAGGTCTGTTTATGAATCGCGTAGGCGATTTGGTGAGGTGCTTGCTACTGAAAGTCCTGTTGATTGTGATGTTGTGATAGCTGTTCCTGATTCTGGTGTTGTGGCTGCGCTTGGTTATGCTGCGAAAGCCGGTGTTCCTTTTCAGCAAGGGTTGATTAGGTCACACTATGTAGGAAGAACTTTCATTGAGCCGTCTCAGAAGATTAGGGACTTTGGTGTGAAGTTGAAGCTTTCACCGGTCCGTGCTGTGCTTGAAGGTAAAAGGGTTGTTGTCGTTGATGATTCAATTGTGAGGGGAACAACATCGTCGAAGATTGTGAGGTTGCTTAAGGAAGCTGGTGCAAAAGAAGTTCACATGAGGATTGCAAGTCCACCAATTATTGGTTCTTGTTACTATGGTGTGGATACACCTAGCTCAGAGGAGTTGATATCTAATAGGATGAGTGTTGAAGAAATAAGGGATTTTATTGGATCCGATTCACTTGCTTTTCTTCCAATCAATAGCTTGCATAGTATGTTGGGTAATGATTCTCCTAATTTTTGTTATGCTTGTTTCTCTGGGAAATACCCTGTTGAACCAAGAGAGCTCAAGGTTAAGAGGGTTGGTGACTTTGTTGATGATGGTTTGAATGGGAGTTTGGAACAAATTGATGGTGGTTGGGTTCAAGCCAACAGAAATCCAAAAGAGGTGAATACTGCTACTGGTCTTTGA
Protein sequence

>MS.gene50697.t1

MAAAQTLSSSLSKPSLLPPNNNRFFDTSLSKSTLPSLSDTRKPLFLTSSKNPISDIILSNKNHPQDEDVFINDDKPREECGVVGIYGDSEASRLCYLALHALQHRGQEGAGIVTVNNNVLQSITGVGLVSDVFNETKLDQLPGSLAIGHVRYSTAGQSMLKNVQPFVAGYRFGSVGVAHNGNLVNYRTLRAKLEDKGSIFNTTSDTEVVLHLIATSKHRPFILRIVDACEKLEGAYSIVFVTEDKLVAVRDPFGFRPLVMGRRSNGAVVFASETCALDLIEATYEREVFPGEVIVVDNNGIQSLCLMSHPQPKQCIFEHIYFALPNSVVFGRSVYESRRRFGEVLATESPVDCDVVIAVPDSGVVAALGYAAKAGVPFQQGLIRSHYVGRTFIEPSQKIRDFGVKLKLSPVRAVLEGKRVVVVDDSIVRGTTSSKIVRLLKEAGAKEVHMRIASPPIIGSCYYGVDTPSSEELISNRMSVEEIRDFIGSDSLAFLPINSLHSMLGNDSPNFCYACFSGKYPVEPRELKVKRVGDFVDDGLNGSLEQIDGGWVQANRNPKEVNTATGL