Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51566.t1 | RHN76904.1 | 79.1 | 115 | 16 | 2 | 1 | 107 | 1 | 115 | 3.90E-20 | 107.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51566.t1 | A0A396JIC7 | 79.1 | 115 | 16 | 2 | 1 | 107 | 1 | 115 | 2.8e-20 | 107.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04995 | MS.gene51566 | 0.828618 | 8.14E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTCGCCGGATTGCTGAAT+TGG | 0.309332 | 1.4:+8824534 | MS.gene51566:CDS |
| GATCGTCTCCGGTAGCTAAA+TGG | 0.396904 | 1.4:+8824578 | MS.gene51566:intron |
| AAACGCAGCGGCGGAGGATG+AGG | 0.422533 | 1.4:+8824355 | MS.gene51566:CDS |
| GGCCGAAAACGCAGCGGCGG+AGG | 0.424027 | 1.4:+8824349 | MS.gene51566:CDS |
| GGGTGAGGACGAGGATTGTA+AGG | 0.437405 | 1.4:+8824415 | MS.gene51566:CDS |
| CTCGGTGCTGCAAAATTTGA+AGG | 0.452756 | 1.4:+8824647 | MS.gene51566:CDS |
| TAAGAAGGATTCTGATATTG+AGG | 0.461648 | 1.4:+8824388 | MS.gene51566:CDS |
| TGAGGTGGTGGGTGAGGACG+AGG | 0.480180 | 1.4:+8824406 | MS.gene51566:CDS |
| AGCCTGCGGATTATCGAGAA+TGG | 0.495049 | 1.4:-8824279 | None:intergenic |
| GCCTGCGGATTATCGAGAAT+GGG | 0.496692 | 1.4:-8824278 | None:intergenic |
| AGCTAAATGGTCTCTTCCGC+CGG | 0.506884 | 1.4:+8824591 | MS.gene51566:intron |
| TCCCATTCTCGATAATCCGC+AGG | 0.518135 | 1.4:+8824277 | MS.gene51566:CDS |
| CTCTTCCGCCGGAGTATGAA+CGG | 0.520118 | 1.4:+8824602 | MS.gene51566:CDS |
| AGCGGCGGAGGATGAGGCCG+AGG | 0.521798 | 1.4:+8824361 | MS.gene51566:CDS |
| AGTATCTACTTCTGTGACCT+CGG | 0.524021 | 1.4:+8824629 | MS.gene51566:CDS |
| AGAATCCTTCTTACAATCCT+CGG | 0.527857 | 1.4:-8824378 | None:intergenic |
| GGCGGCCGAAAACGCAGCGG+CGG | 0.533517 | 1.4:+8824346 | MS.gene51566:CDS |
| GGATTCTGATATTGAGGTGG+TGG | 0.537381 | 1.4:+8824394 | MS.gene51566:CDS |
| GCGACTCCAATTCAGCAATC+CGG | 0.545155 | 1.4:-8824540 | None:intergenic |
| TGACGATGAAGATCGTAAGA+TGG | 0.545924 | 1.4:+8824469 | MS.gene51566:CDS |
| TCCGCCGGAGTATGAACGGC+CGG | 0.547855 | 1.4:+8824606 | MS.gene51566:CDS |
| TGATATTGAGGTGGTGGGTG+AGG | 0.560426 | 1.4:+8824400 | MS.gene51566:CDS |
| TGATAATAAAACGACGGAGG+AGG | 0.569750 | 1.4:+8824445 | MS.gene51566:CDS |
| TACTCCGGCCGTTCATACTC+CGG | 0.584329 | 1.4:-8824610 | None:intergenic |
| TGAGGCCGAGGATTGTAAGA+AGG | 0.586604 | 1.4:+8824373 | MS.gene51566:CDS |
| TGAGGACGAGGATTGTAAGG+TGG | 0.605028 | 1.4:+8824418 | MS.gene51566:CDS |
| AATTCACGTCGGTTATCATA+CGG | 0.606103 | 1.4:+8824325 | MS.gene51566:CDS |
| GAAGGATTCTGATATTGAGG+TGG | 0.624310 | 1.4:+8824391 | MS.gene51566:CDS |
| GGTCACAGAAGTAGATACTC+CGG | 0.644247 | 1.4:-8824625 | None:intergenic |
| GATCGTAAGATGGATTCTGA+TGG | 0.644883 | 1.4:+8824479 | MS.gene51566:CDS |
| TACGGCGGCCGAAAACGCAG+CGG | 0.665004 | 1.4:+8824343 | MS.gene51566:CDS |
| TCCAATTCAGCAATCCGGCG+AGG | 0.671477 | 1.4:-8824535 | None:intergenic |
| TCCGGCCGTTCATACTCCGG+CGG | 0.676143 | 1.4:-8824607 | None:intergenic |
| TTCTGATAATAAAACGACGG+AGG | 0.680167 | 1.4:+8824442 | MS.gene51566:CDS |
| AAAATGTTGCGAATCAAACA+CGG | 0.685323 | 1.4:-8824504 | None:intergenic |
| GGATTGCTGAATTGGAGTCG+CGG | 0.688638 | 1.4:+8824542 | MS.gene51566:CDS |
| GATTCTGATATTGAGGTGGT+GGG | 0.689311 | 1.4:+8824395 | MS.gene51566:CDS |
| GGATTCTGATAATAAAACGA+CGG | 0.719251 | 1.4:+8824439 | MS.gene51566:CDS |
| TCACGTCGGTTATCATACGG+CGG | 0.733450 | 1.4:+8824328 | MS.gene51566:CDS |
| GCTGCGGACGAAATTCACGT+CGG | 0.753969 | 1.4:+8824314 | MS.gene51566:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGTTGCGAATCAAACA+CGG | - | chr1.4:8824507-8824526 | None:intergenic | 30.0% | |
| GGATTCTGATAATAAAACGA+CGG | + | chr1.4:8824439-8824458 | MS.gene51566:CDS | 30.0% | |
| TAAGAAGGATTCTGATATTG+AGG | + | chr1.4:8824388-8824407 | MS.gene51566:CDS | 30.0% | |
| AGAATCCTTCTTACAATCCT+CGG | - | chr1.4:8824381-8824400 | None:intergenic | 35.0% | |
| TTCTGATAATAAAACGACGG+AGG | + | chr1.4:8824442-8824461 | MS.gene51566:CDS | 35.0% | |
| ! | AATTCACGTCGGTTATCATA+CGG | + | chr1.4:8824325-8824344 | MS.gene51566:CDS | 35.0% |
| AGTATCTACTTCTGTGACCT+CGG | + | chr1.4:8824629-8824648 | MS.gene51566:CDS | 40.0% | |
| GAAGGATTCTGATATTGAGG+TGG | + | chr1.4:8824391-8824410 | MS.gene51566:CDS | 40.0% | |
| GATTCTGATATTGAGGTGGT+GGG | + | chr1.4:8824395-8824414 | MS.gene51566:CDS | 40.0% | |
| TGACGATGAAGATCGTAAGA+TGG | + | chr1.4:8824469-8824488 | MS.gene51566:CDS | 40.0% | |
| TGATAATAAAACGACGGAGG+AGG | + | chr1.4:8824445-8824464 | MS.gene51566:CDS | 40.0% | |
| ! | GATCGTAAGATGGATTCTGA+TGG | + | chr1.4:8824479-8824498 | MS.gene51566:CDS | 40.0% |
| !! | GCAGAATTTTTCAAAGCCTG+CGG | - | chr1.4:8824296-8824315 | None:intergenic | 40.0% |
| !! | GGCTTTGAAAAATTCTGCTG+CGG | + | chr1.4:8824298-8824317 | MS.gene51566:CDS | 40.0% |
| GGAAGAGACCATTTAGCTAC+CGG | - | chr1.4:8824589-8824608 | None:intergenic | 45.0% | |
| GGATTCTGATATTGAGGTGG+TGG | + | chr1.4:8824394-8824413 | MS.gene51566:CDS | 45.0% | |
| GGTCACAGAAGTAGATACTC+CGG | - | chr1.4:8824628-8824647 | None:intergenic | 45.0% | |
| TGACTGTAAGTGATCGTCTC+CGG | + | chr1.4:8824567-8824586 | MS.gene51566:intron | 45.0% | |
| ! | CTTCAAATTTTGCAGCACCG+AGG | - | chr1.4:8824649-8824668 | None:intergenic | 45.0% |
| !! | CTCGGTGCTGCAAAATTTGA+AGG | + | chr1.4:8824647-8824666 | MS.gene51566:CDS | 45.0% |
| AGCTAAATGGTCTCTTCCGC+CGG | + | chr1.4:8824591-8824610 | MS.gene51566:intron | 50.0% | |
| GATCGTCTCCGGTAGCTAAA+TGG | + | chr1.4:8824578-8824597 | MS.gene51566:intron | 50.0% | |
| GCGACTCCAATTCAGCAATC+CGG | - | chr1.4:8824543-8824562 | None:intergenic | 50.0% | |
| GGATTGCTGAATTGGAGTCG+CGG | + | chr1.4:8824542-8824561 | MS.gene51566:CDS | 50.0% | |
| TCCCATTCTCGATAATCCGC+AGG | + | chr1.4:8824277-8824296 | MS.gene51566:CDS | 50.0% | |
| TGAGGACGAGGATTGTAAGG+TGG | + | chr1.4:8824418-8824437 | MS.gene51566:CDS | 50.0% | |
| TGAGGCCGAGGATTGTAAGA+AGG | + | chr1.4:8824373-8824392 | MS.gene51566:CDS | 50.0% | |
| ! | AGCCTGCGGATTATCGAGAA+TGG | - | chr1.4:8824282-8824301 | None:intergenic | 50.0% |
| ! | CATTTTCACTCTCACCTCGC+CGG | + | chr1.4:8824521-8824540 | MS.gene51566:CDS | 50.0% |
| ! | GCCTGCGGATTATCGAGAAT+GGG | - | chr1.4:8824281-8824300 | None:intergenic | 50.0% |
| ! | TCACGTCGGTTATCATACGG+CGG | + | chr1.4:8824328-8824347 | MS.gene51566:CDS | 50.0% |
| !! | TGATATTGAGGTGGTGGGTG+AGG | + | chr1.4:8824400-8824419 | MS.gene51566:CDS | 50.0% |
| ACCTCGCCGGATTGCTGAAT+TGG | + | chr1.4:8824534-8824553 | MS.gene51566:CDS | 55.0% | |
| CTCTTCCGCCGGAGTATGAA+CGG | + | chr1.4:8824602-8824621 | MS.gene51566:CDS | 55.0% | |
| GCTGCGGACGAAATTCACGT+CGG | + | chr1.4:8824314-8824333 | MS.gene51566:CDS | 55.0% | |
| GGGTGAGGACGAGGATTGTA+AGG | + | chr1.4:8824415-8824434 | MS.gene51566:CDS | 55.0% | |
| TACTCCGGCCGTTCATACTC+CGG | - | chr1.4:8824613-8824632 | None:intergenic | 55.0% | |
| TCCAATTCAGCAATCCGGCG+AGG | - | chr1.4:8824538-8824557 | None:intergenic | 55.0% | |
| ! | ATCCTCCGCCGCTGCGTTTT+CGG | - | chr1.4:8824354-8824373 | None:intergenic | 60.0% |
| AAACGCAGCGGCGGAGGATG+AGG | + | chr1.4:8824355-8824374 | MS.gene51566:CDS | 65.0% | |
| TACGGCGGCCGAAAACGCAG+CGG | + | chr1.4:8824343-8824362 | MS.gene51566:CDS | 65.0% | |
| TCCGCCGGAGTATGAACGGC+CGG | + | chr1.4:8824606-8824625 | MS.gene51566:CDS | 65.0% | |
| TCCGGCCGTTCATACTCCGG+CGG | - | chr1.4:8824610-8824629 | None:intergenic | 65.0% | |
| !! | TGAGGTGGTGGGTGAGGACG+AGG | + | chr1.4:8824406-8824425 | MS.gene51566:CDS | 65.0% |
| AGCGGCGGAGGATGAGGCCG+AGG | + | chr1.4:8824361-8824380 | MS.gene51566:CDS | 75.0% | |
| GGCCGAAAACGCAGCGGCGG+AGG | + | chr1.4:8824349-8824368 | MS.gene51566:CDS | 75.0% | |
| GGCGGCCGAAAACGCAGCGG+CGG | + | chr1.4:8824346-8824365 | MS.gene51566:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 8824269 | 8824697 | 8824269 | ID=MS.gene51566 |
| chr1.4 | mRNA | 8824269 | 8824697 | 8824269 | ID=MS.gene51566.t1;Parent=MS.gene51566 |
| chr1.4 | exon | 8824269 | 8824571 | 8824269 | ID=MS.gene51566.t1.exon1;Parent=MS.gene51566.t1 |
| chr1.4 | CDS | 8824269 | 8824571 | 8824269 | ID=cds.MS.gene51566.t1;Parent=MS.gene51566.t1 |
| chr1.4 | exon | 8824593 | 8824697 | 8824593 | ID=MS.gene51566.t1.exon2;Parent=MS.gene51566.t1 |
| chr1.4 | CDS | 8824593 | 8824697 | 8824593 | ID=cds.MS.gene51566.t1;Parent=MS.gene51566.t1 |
| Gene Sequence |
| Protein sequence |
