AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52419

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52419.t1 MTR_2g436860 97.931 145 2 1 3 147 5 148 6.59e-97 276
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52419.t1 AT4G15790 54.967 151 54 3 3 147 5 147 8.33e-46 146
MS.gene52419.t1 AT4G15790 45.109 184 54 4 3 147 5 180 7.56e-39 130

Find 25 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCCTCCTTTGGTAATAC+TGG 0.187408 2.3:-54542355 None:intergenic
ATGCTAGCTTAAGAAACATT+AGG 0.285076 2.3:+54542166 MS.gene52419:CDS
CGACATTAAGTGTTTGCTTA+AGG 0.328554 2.3:-54541986 None:intergenic
GAATCAAGATCCGAGCTTCT+TGG 0.345358 2.3:+54539141 MS.gene52419:CDS
TGCTAGCTTAAGAAACATTA+GGG 0.356810 2.3:+54542167 MS.gene52419:CDS
TAGAATTCAAAGTTTGAAAC+AGG 0.385367 2.3:+54539164 MS.gene52419:CDS
AGAGAAGGAGCTGTGGAATC+AGG 0.387365 2.3:-54538864 None:intergenic
ATTACTAGGATTTGCAAGGT+TGG 0.464153 2.3:+54539287 MS.gene52419:intron
GCAAGGTTGGAGGTCAAAGT+TGG 0.498307 2.3:+54539300 MS.gene52419:CDS
GAGAGGGAGAGAAGGAGCTG+TGG 0.502514 2.3:-54538871 None:intergenic
GTGGATGAGAGAGGGAGAGA+AGG 0.522013 2.3:-54538879 None:intergenic
ACTAGGATTTGCAAGGTTGG+AGG 0.527934 2.3:+54539290 MS.gene52419:intron
TTGAATTCTACCAAGAAGCT+CGG 0.531172 2.3:-54539151 None:intergenic
AATGATTACTAGGATTTGCA+AGG 0.536838 2.3:+54539283 MS.gene52419:intron
CTCTTGTTGCTGCTGAAGAG+TGG 0.539219 2.3:-54542136 None:intergenic
GCCAGTATTACCAAAGGAGG+AGG 0.543767 2.3:+54542354 MS.gene52419:CDS
GTGGAATCAGGTGAAGACAT+TGG 0.550371 2.3:-54538852 None:intergenic
ATTAGGAATTTCAAGATCTG+AGG 0.557465 2.3:+54542112 MS.gene52419:intron
AAAGTTGGATACACAAGTCA+AGG 0.560365 2.3:+54539315 MS.gene52419:CDS
GACTCACAGTGGATGAGAGA+GGG 0.585993 2.3:-54538887 None:intergenic
CACTCTTCAGCAGCAACAAG+AGG 0.604456 2.3:+54542137 MS.gene52419:CDS
GGACTCACAGTGGATGAGAG+AGG 0.605991 2.3:-54538888 None:intergenic
GCAGCCAGTATTACCAAAGG+AGG 0.620030 2.3:+54542351 MS.gene52419:CDS
AGAGCAGCCAGTATTACCAA+AGG 0.629734 2.3:+54542348 MS.gene52419:CDS
TAAGATAGAAGAAATTGTCA+AGG 0.632308 2.3:+54542324 MS.gene52419:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATATAAAATATAAAAGA+AGG - chr2.3:54541056-54541075 None:intergenic 10.0%
!! AATAAAACTATAACTTTACT+TGG - chr2.3:54540655-54540674 None:intergenic 15.0%
!! AATATAAAATTGAATTGGAT+AGG - chr2.3:54540541-54540560 None:intergenic 15.0%
!! AATTAATCACAAAAACATTA+TGG + chr2.3:54539070-54539089 MS.gene52419:intron 15.0%
!! ATTATTAAAAAAGAGAGAAA+AGG - chr2.3:54539706-54539725 None:intergenic 15.0%
!! GAAAAACAATAGAATAAAAT+GGG - chr2.3:54538935-54538954 None:intergenic 15.0%
!! TGAAAAACAATAGAATAAAA+TGG - chr2.3:54538936-54538955 None:intergenic 15.0%
!!! TTATTTTATGACCAAATATT+AGG + chr2.3:54539988-54540007 MS.gene52419:intron 15.0%
!!! TTGTTTTTGAAAACAAAATT+AGG + chr2.3:54542095-54542114 MS.gene52419:intron 15.0%
!!! TTTTTGTTTTGTTTTGTTTT+TGG + chr2.3:54539253-54539272 MS.gene52419:intron 15.0%
!! AAATACCAAATGTATGTATA+AGG - chr2.3:54540758-54540777 None:intergenic 20.0%
!! AACTAGCTATACTTAATTAA+GGG - chr2.3:54541388-54541407 None:intergenic 20.0%
!! AAGAAAAAAAACTTCACATT+AGG - chr2.3:54540785-54540804 None:intergenic 20.0%
!! AATGATTAAGAAATACCTTA+TGG - chr2.3:54540179-54540198 None:intergenic 20.0%
!! AATTAATGAATCACATGAAA+AGG - chr2.3:54539195-54539214 None:intergenic 20.0%
!! ACAGACATAAAATAAAGATA+AGG - chr2.3:54541746-54541765 None:intergenic 20.0%
!! ACTCTGTTAAAAGTTAAAAA+TGG - chr2.3:54541421-54541440 None:intergenic 20.0%
!! AGAAAAAAAACTTCACATTA+GGG - chr2.3:54540784-54540803 None:intergenic 20.0%
!! CAGACATAAAATAAAGATAA+GGG - chr2.3:54541745-54541764 None:intergenic 20.0%
!! CTCTGTTAAAAGTTAAAAAT+GGG - chr2.3:54541420-54541439 None:intergenic 20.0%
!! GTGCTAATATAAAATTGAAT+TGG - chr2.3:54540546-54540565 None:intergenic 20.0%
!! TAACTAAATTAAGTATTCGA+AGG - chr2.3:54542230-54542249 None:intergenic 20.0%
!! TGATTCATTAATTAGTTCAT+GGG + chr2.3:54539202-54539221 MS.gene52419:intron 20.0%
!! TTATACATACATTTGGTATT+TGG + chr2.3:54540757-54540776 MS.gene52419:intron 20.0%
!! TTATTCCTTATACATACATT+TGG + chr2.3:54540750-54540769 MS.gene52419:intron 20.0%
!! TTCTTAATCATTGTATCAAA+AGG + chr2.3:54540187-54540206 MS.gene52419:intron 20.0%
!!! AAGTTTTTTTTCTTTCTTTC+AGG + chr2.3:54540791-54540810 MS.gene52419:intron 20.0%
!!! AATTGTTTTTACAATCAAAG+AGG + chr2.3:54540870-54540889 MS.gene52419:intron 20.0%
!!! AGTTTTTTTTCTTTCTTTCA+GGG + chr2.3:54540792-54540811 MS.gene52419:intron 20.0%
!!! ATGTTTATTTTTGTTATCTC+AGG + chr2.3:54539110-54539129 MS.gene52419:intron 20.0%
!!! ATTGTTTTTACAATCAAAGA+GGG + chr2.3:54540871-54540890 MS.gene52419:intron 20.0%
!!! CATAATAAAGATATTTCTAC+AGG - chr2.3:54541462-54541481 None:intergenic 20.0%
! AAAAAAGAGAGAAAAGGTTT+AGG - chr2.3:54539700-54539719 None:intergenic 25.0%
! AAAAAGAGAGAAAAGGTTTA+GGG - chr2.3:54539699-54539718 None:intergenic 25.0%
! AACAAAAATAAACATGACGT+TGG - chr2.3:54539105-54539124 None:intergenic 25.0%
! AACAAAAATTCAGCTAAGAA+TGG - chr2.3:54539241-54539260 None:intergenic 25.0%
! AACATTTCATCTTTAGTGAT+AGG - chr2.3:54539442-54539461 None:intergenic 25.0%
! ACTTATAACATATCCATAAG+TGG - chr2.3:54540977-54540996 None:intergenic 25.0%
! ATAGACATGATAGAACATTA+TGG + chr2.3:54541825-54541844 MS.gene52419:intron 25.0%
! ATTAAGGGTAGAAAATACTT+TGG - chr2.3:54541373-54541392 None:intergenic 25.0%
! CAAAACAACAAATTTATCAC+TGG - chr2.3:54541922-54541941 None:intergenic 25.0%
! CAACTAGCTATACTTAATTA+AGG - chr2.3:54541389-54541408 None:intergenic 25.0%
! CTTAATTAAGTATAGCTAGT+TGG + chr2.3:54541387-54541406 MS.gene52419:intron 25.0%
! GTGATTCATTAATTAGTTCA+TGG + chr2.3:54539201-54539220 MS.gene52419:intron 25.0%
! GTGTATATTTCAGTTTACAA+TGG - chr2.3:54541298-54541317 None:intergenic 25.0%
! TAAGATAGAAGAAATTGTCA+AGG + chr2.3:54542324-54542343 MS.gene52419:CDS 25.0%
! TTAAGGGTAGAAAATACTTT+GGG - chr2.3:54541372-54541391 None:intergenic 25.0%
! TTAGCACAAAATACAGATTT+AGG + chr2.3:54540559-54540578 MS.gene52419:intron 25.0%
! TTGCTATAAAGGTTATTCAA+GGG + chr2.3:54540714-54540733 MS.gene52419:intron 25.0%
! TTTCATAGGGTAAACAAATA+TGG - chr2.3:54539642-54539661 None:intergenic 25.0%
!! AAAGAAAAGCATAAAAGAAG+AGG - chr2.3:54538912-54538931 None:intergenic 25.0%
!! AGTTAACTGTATACTGATTA+CGG + chr2.3:54541345-54541364 MS.gene52419:intron 25.0%
!! TAGAATTCAAAGTTTGAAAC+AGG + chr2.3:54539164-54539183 MS.gene52419:CDS 25.0%
!! TGTTTCTATAAGCTATCTTA+AGG + chr2.3:54540928-54540947 MS.gene52419:intron 25.0%
!! TTGATTTTATCCTTTCTGTT+TGG - chr2.3:54540316-54540335 None:intergenic 25.0%
!! TTTGCTATAAAGGTTATTCA+AGG + chr2.3:54540713-54540732 MS.gene52419:intron 25.0%
!! TTTTAATAATAACTCCCAAG+TGG + chr2.3:54539716-54539735 MS.gene52419:intron 25.0%
!!! ATCTTCTATGCTTTTTTTCT+TGG + chr2.3:54540143-54540162 MS.gene52419:intron 25.0%
!!! GTTTTTTTTCTTTCTTTCAG+GGG + chr2.3:54540793-54540812 MS.gene52419:intron 25.0%
!!! TTCTGAAATGGAAAAAAAGA+AGG - chr2.3:54538985-54539004 None:intergenic 25.0%
!!! TTCTTTTTTTCCATTTCAGA+AGG + chr2.3:54538984-54539003 MS.gene52419:intron 25.0%
AAAATGGTTGTCATGTTTGA+GGG - chr2.3:54541200-54541219 None:intergenic 30.0%
AAATCACGGCAAATGATAAT+TGG + chr2.3:54539906-54539925 MS.gene52419:intron 30.0%
AATAACCTTTATAGCAAACG+TGG - chr2.3:54540711-54540730 None:intergenic 30.0%
AATCACGGCAAATGATAATT+GGG + chr2.3:54539907-54539926 MS.gene52419:intron 30.0%
AATGATTACTAGGATTTGCA+AGG + chr2.3:54539283-54539302 MS.gene52419:intron 30.0%
AGAACATTGATCATACAAAG+TGG - chr2.3:54539969-54539988 None:intergenic 30.0%
AGATCTAGTGATTAATGAGT+TGG + chr2.3:54541800-54541819 MS.gene52419:intron 30.0%
AGCAGAAATTTCACTAACAA+AGG - chr2.3:54541517-54541536 None:intergenic 30.0%
ATAACCTTTATAGCAAACGT+GGG - chr2.3:54540710-54540729 None:intergenic 30.0%
ATATGGTATGTTTCGATTGA+TGG - chr2.3:54539625-54539644 None:intergenic 30.0%
ATGCACTTACTTATTTATGC+TGG + chr2.3:54540026-54540045 MS.gene52419:intron 30.0%
ATGGAAAAAAAGAAGGTAAG+CGG - chr2.3:54538978-54538997 None:intergenic 30.0%
ATGTTTGTAGCCAAATTTCA+TGG + chr2.3:54540497-54540516 MS.gene52419:intron 30.0%
ATTAGGAATTTCAAGATCTG+AGG + chr2.3:54542112-54542131 MS.gene52419:intron 30.0%
CATAACTCAAATCTACAAGT+TGG + chr2.3:54540457-54540476 MS.gene52419:intron 30.0%
CCAAATTTCATGGTCTAATA+GGG + chr2.3:54540507-54540526 MS.gene52419:intron 30.0%
GTAAAACAGTATCGCATATA+GGG + chr2.3:54541150-54541169 MS.gene52419:intron 30.0%
GTATCAAAAGGAAAGTTTCT+AGG + chr2.3:54540199-54540218 MS.gene52419:intron 30.0%
GTTTGTACTCTGTATATCAT+AGG - chr2.3:54539797-54539816 None:intergenic 30.0%
TACAAGGACCAAAATGAAAA+TGG - chr2.3:54541216-54541235 None:intergenic 30.0%
TATAAAGGTTATTCAAGGGA+TGG + chr2.3:54540718-54540737 MS.gene52419:intron 30.0%
TATGTTGACCATATATGTCT+GGG + chr2.3:54541269-54541288 MS.gene52419:intron 30.0%
TCCAATTTCCTCAAAAATTG+CGG + chr2.3:54539017-54539036 MS.gene52419:CDS 30.0%
TCCAGTTACTATGAAATACA+AGG - chr2.3:54541232-54541251 None:intergenic 30.0%
TCCTTGTATTTCATAGTAAC+TGG + chr2.3:54541228-54541247 MS.gene52419:intron 30.0%
TGGATCTGTTAATGATTACT+AGG + chr2.3:54539273-54539292 MS.gene52419:intron 30.0%
TGTAAAACAGTATCGCATAT+AGG + chr2.3:54541149-54541168 MS.gene52419:intron 30.0%
TGTATTTCATAGTAACTGGA+TGG + chr2.3:54541232-54541251 MS.gene52419:intron 30.0%
TTATGTTGACCATATATGTC+TGG + chr2.3:54541268-54541287 MS.gene52419:intron 30.0%
TTGTTAGTGAAATTTCTGCT+TGG + chr2.3:54541517-54541536 MS.gene52419:intron 30.0%
! AATATAAAAGAAGGCACGAT+TGG - chr2.3:54541047-54541066 None:intergenic 30.0%
! ACATATCCATAAGTGGTTTT+CGG - chr2.3:54540970-54540989 None:intergenic 30.0%
! ATGCTAGCTTAAGAAACATT+AGG + chr2.3:54542166-54542185 MS.gene52419:CDS 30.0%
! ATGTTTCGATTGATGGTATA+AGG - chr2.3:54539618-54539637 None:intergenic 30.0%
! ATTTAGGGTATGTTTGAGAA+TGG + chr2.3:54539556-54539575 MS.gene52419:intron 30.0%
! CAACTTGTAGATTTGAGTTA+TGG - chr2.3:54540459-54540478 None:intergenic 30.0%
! CATGACAACCATTTTCATTT+TGG + chr2.3:54541205-54541224 MS.gene52419:intron 30.0%
! CTAAACTCTTCTTTTACAAC+TGG + chr2.3:54541661-54541680 MS.gene52419:intron 30.0%
! GTAATATTCTCCTTCTGAAA+TGG - chr2.3:54538997-54539016 None:intergenic 30.0%
! GTATAATACCGCAATTTTTG+AGG - chr2.3:54539028-54539047 None:intergenic 30.0%
! TGCTAGCTTAAGAAACATTA+GGG + chr2.3:54542167-54542186 MS.gene52419:CDS 30.0%
! TTTTAGCAGAGTGAAATGTA+GGG + chr2.3:54541119-54541138 MS.gene52419:intron 30.0%
!! CTTTTAGTTGCATTTGAGTT+AGG + chr2.3:54539476-54539495 MS.gene52419:intron 30.0%
!! TAGGGTAAGTATCGAAAATT+GGG + chr2.3:54542185-54542204 MS.gene52419:intron 30.0%
!! TTAGGGTAAGTATCGAAAAT+TGG + chr2.3:54542184-54542203 MS.gene52419:intron 30.0%
!! TTATACCATGTTTGTTTCTG+CGG + chr2.3:54539822-54539841 MS.gene52419:intron 30.0%
!! TTGGAGCTTTGTTAAAATCA+CGG + chr2.3:54539892-54539911 MS.gene52419:intron 30.0%
!!! CTTGTGTCTTGTTTGTTTTT+TGG + chr2.3:54541626-54541645 MS.gene52419:intron 30.0%
AAAGCATAGAAGATGTGATG+TGG - chr2.3:54540137-54540156 None:intergenic 35.0%
AAAGTTGGATACACAAGTCA+AGG + chr2.3:54539315-54539334 MS.gene52419:CDS 35.0%
AAATGGTTGTCATGTTTGAG+GGG - chr2.3:54541199-54541218 None:intergenic 35.0%
AACAAACAAGACACAAGAAG+AGG - chr2.3:54541623-54541642 None:intergenic 35.0%
AAGCTCCAAATAGTAACTGT+TGG - chr2.3:54539881-54539900 None:intergenic 35.0%
AATAAGCCGAAAACCACTTA+TGG + chr2.3:54540961-54540980 MS.gene52419:intron 35.0%
ACCCTATGAAACAACAATGA+AGG + chr2.3:54539651-54539670 MS.gene52419:intron 35.0%
ATTACTAGGATTTGCAAGGT+TGG + chr2.3:54539287-54539306 MS.gene52419:intron 35.0%
CACATCACTCTCCTAATATT+TGG - chr2.3:54540002-54540021 None:intergenic 35.0%
CATAACAACAATGACAGTGA+AGG - chr2.3:54540239-54540258 None:intergenic 35.0%
CCCTATGAAACAACAATGAA+GGG + chr2.3:54539652-54539671 MS.gene52419:intron 35.0%
CCCTATTAGACCATGAAATT+TGG - chr2.3:54540510-54540529 None:intergenic 35.0%
CGACATTAAGTGTTTGCTTA+AGG - chr2.3:54541989-54542008 None:intergenic 35.0%
CTATAATCGAGACAACCAAA+AGG - chr2.3:54540630-54540649 None:intergenic 35.0%
GAAAATGGTTGTCATGTTTG+AGG - chr2.3:54541201-54541220 None:intergenic 35.0%
GCCAAATTTCATGGTCTAAT+AGG + chr2.3:54540506-54540525 MS.gene52419:intron 35.0%
TAATAATAACTCCCAAGTGG+AGG + chr2.3:54539719-54539738 MS.gene52419:intron 35.0%
TACAAAGTGGCTGTTTGATT+TGG - chr2.3:54539956-54539975 None:intergenic 35.0%
TCTAACAAATAGTGCGACAA+GGG - chr2.3:54541098-54541117 None:intergenic 35.0%
TCTGACCAACAGTTACTATT+TGG + chr2.3:54539873-54539892 MS.gene52419:intron 35.0%
TTGAATTCTACCAAGAAGCT+CGG - chr2.3:54539154-54539173 None:intergenic 35.0%
! GACAGTTTTTCCAAACAGAA+AGG + chr2.3:54540303-54540322 MS.gene52419:intron 35.0%
! TAGTTGCATTTGAGTTAGGA+AGG + chr2.3:54539480-54539499 MS.gene52419:intron 35.0%
! TGGCATCAAGTTGTACAATT+TGG + chr2.3:54540098-54540117 MS.gene52419:intron 35.0%
! TTTACAATCAAAGAGGGCAT+TGG + chr2.3:54540877-54540896 MS.gene52419:intron 35.0%
! TTTCGATTGATGGTATAAGG+TGG - chr2.3:54539615-54539634 None:intergenic 35.0%
!! AATTTCTGCTTGGTGTTGAT+AGG + chr2.3:54541527-54541546 MS.gene52419:intron 35.0%
!! ACCGCAATTTTTGAGGAAAT+TGG - chr2.3:54539021-54539040 None:intergenic 35.0%
!! ATTTCTGCTTGGTGTTGATA+GGG + chr2.3:54541528-54541547 MS.gene52419:intron 35.0%
!! CCCTTCATTGTTGTTTCATA+GGG - chr2.3:54539655-54539674 None:intergenic 35.0%
!! GTAACTGGATGGCTATTTTA+TGG + chr2.3:54541243-54541262 MS.gene52419:intron 35.0%
!! GTTTTAGCAGAGTGAAATGT+AGG + chr2.3:54541118-54541137 MS.gene52419:intron 35.0%
!! TTACAATCAAAGAGGGCATT+GGG + chr2.3:54540878-54540897 MS.gene52419:intron 35.0%
AAGCCAAACACCATCACTTT+GGG - chr2.3:54540425-54540444 None:intergenic 40.0%
AATGGCAACCCAGACATATA+TGG - chr2.3:54541280-54541299 None:intergenic 40.0%
AGTCATAGTCATAGCTCAGA+AGG + chr2.3:54541580-54541599 MS.gene52419:intron 40.0%
ATGTTAACGGCTCCTAGAAT+TGG + chr2.3:54540365-54540384 MS.gene52419:intron 40.0%
CCCTCTCAAACAAAGTCTTA+GGG + chr2.3:54539757-54539776 MS.gene52419:intron 40.0%
CCTAAATTTCCGAGACTAGA+GGG - chr2.3:54539543-54539562 None:intergenic 40.0%
CCTCTAGTCTCGGAAATTTA+GGG + chr2.3:54539541-54539560 MS.gene52419:intron 40.0%
CTAAATTTCCGAGACTAGAG+GGG - chr2.3:54539542-54539561 None:intergenic 40.0%
CTACTTAGTGGGATAAGACT+TGG + chr2.3:54541873-54541892 MS.gene52419:intron 40.0%
CTCTAACAAATAGTGCGACA+AGG - chr2.3:54541099-54541118 None:intergenic 40.0%
CTTGGAGAGTTAACTCCATA+AGG + chr2.3:54540161-54540180 MS.gene52419:intron 40.0%
TAAAAGAAGAGGACTCACAG+TGG - chr2.3:54538901-54538920 None:intergenic 40.0%
TCAAAATGCATTGCAGCTTG+AGG + chr2.3:54542261-54542280 MS.gene52419:intron 40.0%
TGATAATCTCTCCTCCACTT+GGG - chr2.3:54539733-54539752 None:intergenic 40.0%
TGGTATAAGGTGGAATGAAG+CGG - chr2.3:54539605-54539624 None:intergenic 40.0%
TGTTAACGGCTCCTAGAATT+GGG + chr2.3:54540366-54540385 MS.gene52419:intron 40.0%
TTAAGCATCCACCCAATTCT+AGG - chr2.3:54540380-54540399 None:intergenic 40.0%
TTAGCTGAAACCCAAAGTGA+TGG + chr2.3:54540412-54540431 MS.gene52419:intron 40.0%
! AAACCCAAAGTGATGGTGTT+TGG + chr2.3:54540419-54540438 MS.gene52419:intron 40.0%
! ACAACAATGAAGGGCTTTGT+AGG + chr2.3:54539661-54539680 MS.gene52419:intron 40.0%
! ACCCTAAGACTTTGTTTGAG+AGG - chr2.3:54539761-54539780 None:intergenic 40.0%
! ATCACTTTGGGTTTCAGCTA+AGG - chr2.3:54540413-54540432 None:intergenic 40.0%
! ATTTGAGTTAGGAAGGTGGA+GGG + chr2.3:54539487-54539506 MS.gene52419:intron 40.0%
! CAAGTCTTATCCCACTAAGT+AGG - chr2.3:54541875-54541894 None:intergenic 40.0%
! CCCTAAGACTTTGTTTGAGA+GGG - chr2.3:54539760-54539779 None:intergenic 40.0%
! TTGCATTTGAGTTAGGAAGG+TGG + chr2.3:54539483-54539502 MS.gene52419:intron 40.0%
!! GCCCTTCATTGTTGTTTCAT+AGG - chr2.3:54539656-54539675 None:intergenic 40.0%
!! GTCTTCTGGAAATCTCCTTT+TGG + chr2.3:54540612-54540631 MS.gene52419:intron 40.0%
!! TACAATCAAAGAGGGCATTG+GGG + chr2.3:54540879-54540898 MS.gene52419:intron 40.0%
!! TGTGTTCTTTGCTAGTCTTC+TGG + chr2.3:54540598-54540617 MS.gene52419:intron 40.0%
ACGTAGCTGATCCTACTTAG+TGG + chr2.3:54541861-54541880 MS.gene52419:intron 45.0%
ACTAAGTAGGATCAGCTACG+TGG - chr2.3:54541862-54541881 None:intergenic 45.0%
ACTAGGATTTGCAAGGTTGG+AGG + chr2.3:54539290-54539309 MS.gene52419:intron 45.0%
AGAGAAAACCCCTCTAGTCT+CGG + chr2.3:54539531-54539550 MS.gene52419:intron 45.0%
AGAGCAGCCAGTATTACCAA+AGG + chr2.3:54542348-54542367 MS.gene52419:CDS 45.0%
AGCAGAGTGAAATGTAGGGA+CGG + chr2.3:54541123-54541142 MS.gene52419:intron 45.0%
AGTCAGCATAGCGTTACAGA+TGG - chr2.3:54540071-54540090 None:intergenic 45.0%
CAAGCCAAACACCATCACTT+TGG - chr2.3:54540426-54540445 None:intergenic 45.0%
CCCTAAATTTCCGAGACTAG+AGG - chr2.3:54539544-54539563 None:intergenic 45.0%
CCCTCTAGTCTCGGAAATTT+AGG + chr2.3:54539540-54539559 MS.gene52419:intron 45.0%
CGTAGCTGATCCTACTTAGT+GGG + chr2.3:54541862-54541881 MS.gene52419:intron 45.0%
CTGATAATCTCTCCTCCACT+TGG - chr2.3:54539734-54539753 None:intergenic 45.0%
GAATCAAGATCCGAGCTTCT+TGG + chr2.3:54539141-54539160 MS.gene52419:CDS 45.0%
GCCCTCTCAAACAAAGTCTT+AGG + chr2.3:54539756-54539775 MS.gene52419:intron 45.0%
GGGCAACAGCAAACTAAAGA+GGG - chr2.3:54540690-54540709 None:intergenic 45.0%
GTGTTTGATTCTGTTCTCGC+AGG + chr2.3:54541953-54541972 MS.gene52419:intron 45.0%
TGGGCAACAGCAAACTAAAG+AGG - chr2.3:54540691-54540710 None:intergenic 45.0%
! CATTTGAGTTAGGAAGGTGG+AGG + chr2.3:54539486-54539505 MS.gene52419:intron 45.0%
! TCCTCCTCCTTTGGTAATAC+TGG - chr2.3:54542358-54542377 None:intergenic 45.0%
! TCTGTAACGCTATGCTGACT+TGG + chr2.3:54540071-54540090 MS.gene52419:intron 45.0%
! TTGGCATTTTCCTCCTCCTT+TGG - chr2.3:54542367-54542386 None:intergenic 45.0%
! TTTGAGTTAGGAAGGTGGAG+GGG + chr2.3:54539488-54539507 MS.gene52419:intron 45.0%
!! CTGTAACGCTATGCTGACTT+GGG + chr2.3:54540072-54540091 MS.gene52419:intron 45.0%
!! CTTTCTTTCAGGGGAGCATT+TGG + chr2.3:54540802-54540821 MS.gene52419:intron 45.0%
!! GTTGCCCACGTTTGCTATAA+AGG + chr2.3:54540703-54540722 MS.gene52419:intron 45.0%
!! TGGCTGTTTGATTTGGCAGT+GGG - chr2.3:54539949-54539968 None:intergenic 45.0%
!! TTTGATTTGGCAGTGGGAGA+AGG - chr2.3:54539943-54539962 None:intergenic 45.0%
!! AATTAATTAATAATAAAATG+AGG - chr2.3:54539050-54539069 None:intergenic 5.0%
!! ATTTAAAAAAAAAATGTTAA+CGG + chr2.3:54540352-54540371 MS.gene52419:intron 5.0%
AAGGTGGAGGGGAATGCATT+TGG + chr2.3:54539499-54539518 MS.gene52419:intron 50.0%
AGAGAAGGAGCTGTGGAATC+AGG - chr2.3:54538867-54538886 None:intergenic 50.0%
AGGGGAATGCATTTGGGGAA+AGG + chr2.3:54539506-54539525 MS.gene52419:intron 50.0%
AGGTGGAGGGGAATGCATTT+GGG + chr2.3:54539500-54539519 MS.gene52419:intron 50.0%
CACTCTTCAGCAGCAACAAG+AGG + chr2.3:54542137-54542156 MS.gene52419:CDS 50.0%
CTCTTGTTGCTGCTGAAGAG+TGG - chr2.3:54542139-54542158 None:intergenic 50.0%
GACTCACAGTGGATGAGAGA+GGG - chr2.3:54538890-54538909 None:intergenic 50.0%
GCAAGGTTGGAGGTCAAAGT+TGG + chr2.3:54539300-54539319 MS.gene52419:CDS 50.0%
GCAGCCAGTATTACCAAAGG+AGG + chr2.3:54542351-54542370 MS.gene52419:CDS 50.0%
GCCAGTATTACCAAAGGAGG+AGG + chr2.3:54542354-54542373 MS.gene52419:CDS 50.0%
GGGAATGCATTTGGGGAAAG+GGG + chr2.3:54539508-54539527 MS.gene52419:intron 50.0%
GGGGAATGCATTTGGGGAAA+GGG + chr2.3:54539507-54539526 MS.gene52419:intron 50.0%
GTAACTGTTGGTCAGACGTG+TGG - chr2.3:54539869-54539888 None:intergenic 50.0%
TAACGGCTCCTAGAATTGGG+TGG + chr2.3:54540369-54540388 MS.gene52419:intron 50.0%
!! GTGGCTGTTTGATTTGGCAG+TGG - chr2.3:54539950-54539969 None:intergenic 50.0%
CGCAGCCGCAGAAACAAACA+TGG - chr2.3:54539830-54539849 None:intergenic 55.0%
GGACTCACAGTGGATGAGAG+AGG - chr2.3:54538891-54538910 None:intergenic 55.0%
GGTGGAGGGGAATGCATTTG+GGG + chr2.3:54539501-54539520 MS.gene52419:intron 55.0%
GTGGATGAGAGAGGGAGAGA+AGG - chr2.3:54538882-54538901 None:intergenic 55.0%
!! CGCTATGCTGACTTGGGAGT+TGG + chr2.3:54540078-54540097 MS.gene52419:intron 55.0%
GAGAGGGAGAGAAGGAGCTG+TGG - chr2.3:54538874-54538893 None:intergenic 60.0%
TCTGCGGCTGCGCTGGAATA+CGG + chr2.3:54539838-54539857 MS.gene52419:intron 60.0%
!! GTTTGTTTCTGCGGCTGCGC+TGG + chr2.3:54539831-54539850 MS.gene52419:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 54538855 54542402 54538855 ID=MS.gene52419
chr2.3 mRNA 54538855 54542402 54538855 ID=MS.gene52419.t1;Parent=MS.gene52419
chr2.3 exon 54538855 54538902 54538855 ID=MS.gene52419.t1.exon1;Parent=MS.gene52419.t1
chr2.3 CDS 54538855 54538902 54538855 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
chr2.3 exon 54539003 54539038 54539003 ID=MS.gene52419.t1.exon2;Parent=MS.gene52419.t1
chr2.3 CDS 54539003 54539038 54539003 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
chr2.3 exon 54539132 54539185 54539132 ID=MS.gene52419.t1.exon3;Parent=MS.gene52419.t1
chr2.3 CDS 54539132 54539185 54539132 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
chr2.3 exon 54539295 54539348 54539295 ID=MS.gene52419.t1.exon4;Parent=MS.gene52419.t1
chr2.3 CDS 54539295 54539348 54539295 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
chr2.3 exon 54541975 54542028 54541975 ID=MS.gene52419.t1.exon5;Parent=MS.gene52419.t1
chr2.3 CDS 54541975 54542028 54541975 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
chr2.3 exon 54542117 54542188 54542117 ID=MS.gene52419.t1.exon6;Parent=MS.gene52419.t1
chr2.3 CDS 54542117 54542188 54542117 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
chr2.3 exon 54542277 54542402 54542277 ID=MS.gene52419.t1.exon7;Parent=MS.gene52419.t1
chr2.3 CDS 54542277 54542402 54542277 ID=cds.MS.gene52419.t1;Parent=MS.gene52419.t1
Gene Sequence

>MS.gene52419

ATGTCTTCACCTGATTCCACAGCTCCTTCTCTCCCTCTCTCATCCACTGTGAGTCCTCTTCTTTTATGCTTTTCTTTCCCATTTTATTCTATTGTTTTTCATTTTCAGTATTAATAATCACCGCTTACCTTCTTTTTTTCCATTTCAGAAGGAGAATATTACTCCAATTTCCTCAAAAATTGCGGTATTATACCTCATTTTATTATTAATTAATTAATTAATCACAAAAACATTATGGAATGAACTTCCAACGTCATGTTTATTTTTGTTATCTCAGGAACTTAATGAATCAAGATCCGAGCTTCTTGGTAGAATTCAAAGTTTGAAACAGGTAACACCTTTTCATGTGATTCATTAATTAGTTCATGGGTGTGTTTCTGTTTCCATTCTTAGCTGAATTTTTGTTTTGTTTTGTTTTTGGATCTGTTAATGATTACTAGGATTTGCAAGGTTGGAGGTCAAAGTTGGATACACAAGTCAAGGTTTATCGCGATGTAAGTCACTCTTATTAATCACAATCTGCTCAATACTTGATTTTGCTTCTGTTTTGTGTCAACAGCAAGATTATGATTATGATTATGCTTCCTATCACTAAAGATGAAATGTTATGATTCAACACTTCTTTTAGTTGCATTTGAGTTAGGAAGGTGGAGGGGAATGCATTTGGGGAAAGGGGAGAGAAAACCCCTCTAGTCTCGGAAATTTAGGGTATGTTTGAGAATGGATGTTATCTTATTACATTTCATTCCGCTTCATTCCACCTTATACCATCAATCGAAACATACCATATTTGTTTACCCTATGAAACAACAATGAAGGGCTTTGTAGGTTTTTAATAAATCCCTAAACCTTTTCTCTCTTTTTTAATAATAACTCCCAAGTGGAGGAGAGATTATCAGACGCCCTCTCAAACAAAGTCTTAGGGTGCACATAACATAGCCTATGATATACAGAGTACAAACTGATATTATACCATGTTTGTTTCTGCGGCTGCGCTGGAATACGGCTTCACCACACGTCTGACCAACAGTTACTATTTGGAGCTTTGTTAAAATCACGGCAAATGATAATTGGGATGCGAGTTTCCTTCTCCCACTGCCAAATCAAACAGCCACTTTGTATGATCAATGTTCTTATTTTATGACCAAATATTAGGAGAGTGATGTGTCACATGCACTTACTTATTTATGCTGGCTCTGCTTTTTGTATTTGTCCATCTGTAACGCTATGCTGACTTGGGAGTTGGCATCAAGTTGTACAATTTGGTTCATTTACTTATCCACATCACATCTTCTATGCTTTTTTTCTTGGAGAGTTAACTCCATAAGGTATTTCTTAATCATTGTATCAAAAGGAAAGTTTCTAGGCTGTAATATGAAAGCCTTCACTGTCATTGTTGTTATGAATCAGACTGCATTTGATTAAAACTACAGAGATCTCTTGAATATGACAGTTTTTCCAAACAGAAAGGATAAAATCAAGTGTGTTGTATCAATTATTTAAAAAAAAAATGTTAACGGCTCCTAGAATTGGGTGGATGCTTAACAACTGCTTTCCTTAGCTGAAACCCAAAGTGATGGTGTTTGGCTTGTCATGCTCAACCATAACTCAAATCTACAAGTTGGATAACAAATACACGAAAATGTTTGTAGCCAAATTTCATGGTCTAATAGGGTAGTATATCCTATCCAATTCAATTTTATATTAGCACAAAATACAGATTTAGGATATATTCATACAAGTTGTGTTCTTTGCTAGTCTTCTGGAAATCTCCTTTTGGTTGTCTCGATTATAGAACCAAGTAAAGTTATAGTTTTATTTACATATATTGTCCCTCTTTAGTTTGCTGTTGCCCACGTTTGCTATAAAGGTTATTCAAGGGATGGTATATGTTATTATTCCTTATACATACATTTGGTATTTGGTCCCTAATGTGAAGTTTTTTTTCTTTCTTTCAGGGGAGCATTTGGTTAAACAGTTTGATTAAACACATATTCATTAAACATTTATGTATAAATTGTTTTTACAATCAAAGAGGGCATTGGGGTAAACTTCACATAAACTTGAATAGATTGTTTCTATAAGCTATCTTAAGGAGCTTATAAAAATAAGCCGAAAACCACTTATGGATATGTTATAAGTTACTTCTTTAAGTTTTTTCACACACTTGTATAAGCGCTTTAGTTCTTCCAATCGTGCCTTCTTTTATATTTTATATGTACAATTGCTTTTCAATTCATCCCTTGTCGCACTATTTGTTAGAGTTTTAGCAGAGTGAAATGTAGGGACGGAGTTGTAAAACAGTATCGCATATAGGGATTATAAATATTTAATATTTTGTCCCCTCAAACATGACAACCATTTTCATTTTGGTCCTTGTATTTCATAGTAACTGGATGGCTATTTTATGGTGTTATGTTGACCATATATGTCTGGGTTGCCATTGTAAACTGAAATATACACAAGAATTAAATTGAAAATCTTTTCTTGAGTTAACTGTATACTGATTACGGACCCAAAGTATTTTCTACCCTTAATTAAGTATAGCTAGTTGGAAAGAATCCCATTTTTAACTTTTAACAGAGTTAAACAGCTAAAATGAAACCTGTAGAAATATCTTTATTATGCACAGACACGTGTCATATGTTTGCATTGATTACCTTTGTTAGTGAAATTTCTGCTTGGTGTTGATAGGGTTCATAATATCATTCATAATGTGCTCAATAGTCATAGTCATAGCTCAGAAGGATTAATAATGATTTGATCCTCTTCTTGTGTCTTGTTTGTTTTTTGGTTATTGTTGTAGCTAAACTCTTCTTTTACAACTGGTACTAATGTTACTTTAGCTTTATATTCTTCTTCTTTTTTTTTTTTTACAAATACTTTGCCCTTATCTTTATTTTATGTCTGTGCTATTGATATGATATATGAGAAACTATTAAGAAAGATCTAGTGATTAATGAGTTGGTTATAGACATGATAGAACATTATGGTGTCATTTGTTCCACGTAGCTGATCCTACTTAGTGGGATAAGACTTGGTTGTTGTTGTTGTTATATTTATGCCAGTGATAAATTTGTTGTTTTGCAACTATTGATGTGTTTGATTCTGTTCTCGCAGGAGCTGTCTGACCTTAAGCAAACACTTAATGTCGAAGTAGAGCAACTTAGAGCAGTGAGTGTCTTTTTAATTATTATTTTTCTCAAGTGCTCTCTTTCAATTAAAATTCATGTGTGCGATTTGTTTTTGAAAACAAAATTAGGAATTTCAAGATCTGAGGACCACTCTTCAGCAGCAACAAGAGGATGTTAATGCTAGCTTAAGAAACATTAGGGTAAGTATCGAAAATTGGGTTACTTTTGAAAATCACTTCCTTCGAATACTTAATTTAGTTAGTGCACTGACATCAAAATGCATTGCAGCTTGAGGATGTCTCAGCAGATGCAAAGCAAGCTCAGTCACAAGAAACTAAGATAGAAGAAATTGTCAAGGAAGAGCAGCCAGTATTACCAAAGGAGGAGGAAAATGCCAAAATAGCTGAAAATTAA
Protein sequence

>MS.gene52419.t1

MSSPDSTAPSLPLSSTKENITPISSKIAELNESRSELLGRIQSLKQDLQGWRSKLDTQVKVYRDELSDLKQTLNVEVEQLRAEFQDLRTTLQQQQEDVNASLRNIRLEDVSADAKQAQSQETKIEEIVKEEQPVLPKEEENAKIAEN