Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52789.t1 | XP_013462246.1 | 98.1 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 2.30E-47 | 198 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52789.t1 | A0A072V3Q4 | 98.1 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 1.6e-47 | 198.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05595 | MS.gene52789 | -0.819988 | 8.70E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52789.t1 | MTR_3g115060 | 98.131 | 107 | 2 | 0 | 1 | 107 | 1 | 107 | 4.64e-73 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52789.t1 | AT1G20430 | 65.882 | 85 | 29 | 0 | 23 | 107 | 29 | 113 | 1.05e-33 | 113 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTTAAGCATGAAATCTT+TGG | 0.222509 | 3.1:-91069381 | MS.gene52789:CDS |
| CTCTTAAGCATGAAATCTTT+GGG | 0.311239 | 3.1:-91069380 | MS.gene52789:CDS |
| CCGAATGCCGCCGGTGCCGT+TGG | 0.453325 | 3.1:-91069488 | MS.gene52789:CDS |
| CCTCTTGGAGTCCATGGATT+CGG | 0.472053 | 3.1:+91069348 | None:intergenic |
| CCAACGGCACCGGCGGCATT+CGG | 0.480360 | 3.1:+91069488 | None:intergenic |
| TCAGATCATGAGTATCCTCT+TGG | 0.496481 | 3.1:+91069333 | None:intergenic |
| GAGTATCCTCTTGGAGTCCA+TGG | 0.510166 | 3.1:+91069342 | None:intergenic |
| ATTCGGTCGCACTGAGGAGG+AGG | 0.524049 | 3.1:+91069505 | None:intergenic |
| GAAGCTTCTCAAGATTCTAC+TGG | 0.533479 | 3.1:-91069251 | MS.gene52789:CDS |
| GGACAAAAGTACCGAATCCA+TGG | 0.544794 | 3.1:-91069359 | MS.gene52789:CDS |
| CTTCTTCGAACCAACGGCAC+CGG | 0.551741 | 3.1:+91069478 | None:intergenic |
| TAGCTTCTTCTTCGAACCAA+CGG | 0.568765 | 3.1:+91069472 | None:intergenic |
| CTTCGAACCAACGGCACCGG+CGG | 0.570741 | 3.1:+91069481 | None:intergenic |
| GGCATTCGGTCGCACTGAGG+AGG | 0.571642 | 3.1:+91069502 | None:intergenic |
| CACTGAGGAGGAGGAAGACA+TGG | 0.572521 | 3.1:+91069514 | None:intergenic |
| TCAGTGCGACCGAATGCCGC+CGG | 0.579043 | 3.1:-91069497 | MS.gene52789:CDS |
| AAGAGGATACTCATGATCTG+AGG | 0.589972 | 3.1:-91069331 | MS.gene52789:CDS |
| AGAGGATACTCATGATCTGA+GGG | 0.604407 | 3.1:-91069330 | MS.gene52789:CDS |
| CCGAATCCATGGACTCCAAG+AGG | 0.665176 | 3.1:-91069348 | MS.gene52789:CDS |
| GGCGGCATTCGGTCGCACTG+AGG | 0.704327 | 3.1:+91069499 | None:intergenic |
| ATTCATTGAAGAGACTACGA+AGG | 0.718138 | 3.1:+91069213 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTTAAGCATGAAATCTTT+GGG | - | chr3.1:91069343-91069362 | MS.gene52789:CDS | 30.0% | |
| TCTCTTAAGCATGAAATCTT+TGG | - | chr3.1:91069342-91069361 | MS.gene52789:CDS | 30.0% | |
| ! | CTTGATGTTTTTCATACGAT+CGG | + | chr3.1:91069437-91069456 | None:intergenic | 30.0% |
| ATTCATTGAAGAGACTACGA+AGG | + | chr3.1:91069513-91069532 | None:intergenic | 35.0% | |
| ! | ATTCAACTTTTCGCAATGAC+TGG | - | chr3.1:91069313-91069332 | MS.gene52789:CDS | 35.0% |
| AAGAGGATACTCATGATCTG+AGG | - | chr3.1:91069392-91069411 | MS.gene52789:CDS | 40.0% | |
| AGAGGATACTCATGATCTGA+GGG | - | chr3.1:91069393-91069412 | MS.gene52789:CDS | 40.0% | |
| GAAGCTTCTCAAGATTCTAC+TGG | - | chr3.1:91069472-91069491 | MS.gene52789:CDS | 40.0% | |
| TAGCTTCTTCTTCGAACCAA+CGG | + | chr3.1:91069254-91069273 | None:intergenic | 40.0% | |
| TCAGATCATGAGTATCCTCT+TGG | + | chr3.1:91069393-91069412 | None:intergenic | 40.0% | |
| !! | GGACAAAAGTACCGAATCCA+TGG | - | chr3.1:91069364-91069383 | MS.gene52789:CDS | 45.0% |
| CCTCTTGGAGTCCATGGATT+CGG | + | chr3.1:91069378-91069397 | None:intergenic | 50.0% | |
| GAGTATCCTCTTGGAGTCCA+TGG | + | chr3.1:91069384-91069403 | None:intergenic | 50.0% | |
| CCGAATCCATGGACTCCAAG+AGG | - | chr3.1:91069375-91069394 | MS.gene52789:CDS | 55.0% | |
| CTTCTTCGAACCAACGGCAC+CGG | + | chr3.1:91069248-91069267 | None:intergenic | 55.0% | |
| ATTCGGTCGCACTGAGGAGG+AGG | + | chr3.1:91069221-91069240 | None:intergenic | 60.0% | |
| GGCATTCGGTCGCACTGAGG+AGG | + | chr3.1:91069224-91069243 | None:intergenic | 65.0% | |
| TCAGTGCGACCGAATGCCGC+CGG | - | chr3.1:91069226-91069245 | MS.gene52789:CDS | 65.0% | |
| ! | CTTCGAACCAACGGCACCGG+CGG | + | chr3.1:91069245-91069264 | None:intergenic | 65.0% |
| GGCGGCATTCGGTCGCACTG+AGG | + | chr3.1:91069227-91069246 | None:intergenic | 70.0% | |
| !! | CCAACGGCACCGGCGGCATT+CGG | + | chr3.1:91069238-91069257 | None:intergenic | 70.0% |
| !! | CCGAATGCCGCCGGTGCCGT+TGG | - | chr3.1:91069235-91069254 | MS.gene52789:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 91069211 | 91069534 | 91069211 | ID=MS.gene52789 |
| chr3.1 | mRNA | 91069211 | 91069534 | 91069211 | ID=MS.gene52789.t1;Parent=MS.gene52789 |
| chr3.1 | exon | 91069211 | 91069534 | 91069211 | ID=MS.gene52789.t1.exon1;Parent=MS.gene52789.t1 |
| chr3.1 | CDS | 91069211 | 91069534 | 91069211 | ID=cds.MS.gene52789.t1;Parent=MS.gene52789.t1 |
| Gene Sequence |
| Protein sequence |
