Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52817.t1 | AES74062.2 | 92.4 | 368 | 27 | 1 | 9 | 376 | 11 | 377 | 8.20E-177 | 629.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52817.t1 | Q6DYE5 | 39.6 | 364 | 199 | 7 | 25 | 373 | 29 | 386 | 1.7e-58 | 228.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52817.t1 | G7J5U4 | 92.4 | 368 | 27 | 1 | 9 | 376 | 11 | 377 | 5.9e-177 | 629.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049499 | MS.gene52817 | 0.803205 | 3.90E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene52817 | 0.813449 | 2.55E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene52817 | 0.805947 | 1.04E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene52817 | 0.807955 | 3.93E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52817.t1 | MTR_3g114460 | 91.892 | 370 | 29 | 1 | 7 | 376 | 9 | 377 | 0.0 | 663 |
| MS.gene52817.t1 | MTR_8g068520 | 51.862 | 376 | 153 | 9 | 13 | 375 | 17 | 377 | 4.43e-120 | 352 |
| MS.gene52817.t1 | MTR_1g025960 | 51.596 | 376 | 151 | 8 | 11 | 375 | 11 | 366 | 1.34e-116 | 343 |
| MS.gene52817.t1 | MTR_1g100200 | 28.375 | 363 | 205 | 11 | 30 | 372 | 10 | 337 | 1.82e-29 | 116 |
| MS.gene52817.t1 | MTR_1g100200 | 28.453 | 362 | 204 | 11 | 31 | 372 | 14 | 340 | 2.53e-29 | 116 |
| MS.gene52817.t1 | MTR_1g100200 | 28.453 | 362 | 204 | 11 | 31 | 372 | 34 | 360 | 4.48e-29 | 116 |
| MS.gene52817.t1 | MTR_7g084240 | 24.561 | 285 | 179 | 12 | 97 | 376 | 124 | 377 | 9.00e-13 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52817.t1 | AT1G20180 | 40.110 | 364 | 197 | 7 | 25 | 373 | 29 | 386 | 1.43e-69 | 224 |
| MS.gene52817.t1 | AT1G20180 | 36.813 | 364 | 178 | 8 | 25 | 373 | 29 | 355 | 1.59e-58 | 194 |
| MS.gene52817.t1 | AT3G49070 | 29.737 | 380 | 214 | 8 | 12 | 374 | 16 | 359 | 6.03e-36 | 135 |
| MS.gene52817.t1 | AT3G49070 | 30.000 | 380 | 213 | 8 | 12 | 374 | 56 | 399 | 1.30e-35 | 135 |
| MS.gene52817.t1 | AT3G19330 | 25.135 | 370 | 243 | 10 | 12 | 372 | 28 | 372 | 3.51e-12 | 67.8 |
| MS.gene52817.t1 | AT3G19330 | 25.135 | 370 | 243 | 10 | 12 | 372 | 28 | 372 | 3.51e-12 | 67.8 |
| MS.gene52817.t1 | AT3G19250 | 22.835 | 381 | 259 | 11 | 1 | 372 | 1 | 355 | 6.41e-12 | 66.6 |
Find 53 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATTGCCAAACTAACTTTA+TGG | 0.111324 | 3.1:+90687812 | MS.gene52817:CDS |
| TTTGCTTTCCATAGCATAAT+TGG | 0.142059 | 3.1:+90687498 | MS.gene52817:CDS |
| GCGGAAGTTAATAACATTAT+TGG | 0.198214 | 3.1:-90687322 | None:intergenic |
| CGTCAAACTAGATTTGATTA+TGG | 0.245353 | 3.1:+90687159 | MS.gene52817:CDS |
| AAGAGATCAAAGTGGGAAAA+TGG | 0.266547 | 3.1:+90687573 | MS.gene52817:CDS |
| TTATATAGTGATAAATGATT+TGG | 0.292938 | 3.1:+90687653 | MS.gene52817:CDS |
| GATATTTGTGTGAAGAATTA+TGG | 0.298600 | 3.1:+90687738 | MS.gene52817:CDS |
| TGTAACAAAGCTCAAGGATA+TGG | 0.306198 | 3.1:+90686946 | MS.gene52817:CDS |
| ACTGATCCATAAAGTTAGTT+TGG | 0.327126 | 3.1:-90687818 | None:intergenic |
| TATGTAGTTGGTGGATCCTT+TGG | 0.338019 | 3.1:+90687537 | MS.gene52817:CDS |
| GGTTAAGAGGTTAGAGGATG+AGG | 0.399784 | 3.1:+90687692 | MS.gene52817:CDS |
| GCTGCTGCCCCTTATGTAGT+TGG | 0.407925 | 3.1:+90687525 | MS.gene52817:CDS |
| TGCAAGAAAGTTGGAGGAAT+CGG | 0.409959 | 3.1:+90687432 | MS.gene52817:CDS |
| TTCTTTCTTCCGAAGAAGAT+CGG | 0.414371 | 3.1:+90686593 | MS.gene52817:CDS |
| TTTGTAACAATTTCTTGTCT+AGG | 0.414371 | 3.1:-90687039 | None:intergenic |
| TGTTGCGACAAAATCCTTCA+AGG | 0.417769 | 3.1:+90687132 | MS.gene52817:CDS |
| TTAACTCACCCGATCTTCTT+CGG | 0.418614 | 3.1:-90686602 | None:intergenic |
| CAAATTGATGTTGCTGCAAA+AGG | 0.419880 | 3.1:+90687627 | MS.gene52817:CDS |
| TTGCTTTCCATAGCATAATT+GGG | 0.423917 | 3.1:+90687499 | MS.gene52817:CDS |
| AAGAGGTTATGCAAGAAAGT+TGG | 0.425159 | 3.1:+90687423 | MS.gene52817:CDS |
| AAGGATCCACCAACTACATA+AGG | 0.429019 | 3.1:-90687534 | None:intergenic |
| AAGAATTATGGTCATGGTAA+TGG | 0.433359 | 3.1:+90687750 | MS.gene52817:CDS |
| TGAAGAAGAAGAAGTCGAAC+AGG | 0.439516 | 3.1:-90687007 | None:intergenic |
| TAGTTTGACGAACACCTTGA+AGG | 0.455617 | 3.1:-90687146 | None:intergenic |
| TCTTTCTTCCGAAGAAGATC+GGG | 0.456820 | 3.1:+90686594 | MS.gene52817:CDS |
| CCGTATGGTTAAGAGGTTAG+AGG | 0.460969 | 3.1:+90687686 | MS.gene52817:CDS |
| AACTAACTTTATGGATCAGT+TGG | 0.461239 | 3.1:+90687821 | MS.gene52817:CDS |
| CTATGAGCCGTATGGTTAAG+AGG | 0.474901 | 3.1:+90687679 | MS.gene52817:CDS |
| AAATTGATGTTGCTGCAAAA+GGG | 0.477002 | 3.1:+90687628 | MS.gene52817:CDS |
| AGGATCCACCAACTACATAA+GGG | 0.488584 | 3.1:-90687533 | None:intergenic |
| TAGAGGATGAGGTTGAACAT+TGG | 0.517451 | 3.1:+90687703 | MS.gene52817:CDS |
| GAAGAAGAAGAGATCAAAGT+GGG | 0.525471 | 3.1:+90687566 | MS.gene52817:CDS |
| TGAGGTTGAACATTGGAGAG+AGG | 0.530933 | 3.1:+90687710 | MS.gene52817:CDS |
| ACAAAGACATGCTTCTAAGC+TGG | 0.533545 | 3.1:-90687112 | None:intergenic |
| AGGTTATGCAAGAAAGTTGG+AGG | 0.533933 | 3.1:+90687426 | MS.gene52817:CDS |
| CTTCTTCTTCATCAAACCAA+AGG | 0.546523 | 3.1:-90687553 | None:intergenic |
| CAGCTAACCCAATTATGCTA+TGG | 0.556147 | 3.1:-90687506 | None:intergenic |
| GGATCAGTTGGAAGAGCTTG+AGG | 0.556162 | 3.1:+90687833 | MS.gene52817:CDS |
| AATTGATGTTGCTGCAAAAG+GGG | 0.557456 | 3.1:+90687629 | MS.gene52817:CDS |
| GCTGCCCCTTATGTAGTTGG+TGG | 0.559630 | 3.1:+90687528 | MS.gene52817:CDS |
| TAAGGTGCATCATCTTCTAG+TGG | 0.565366 | 3.1:+90687077 | MS.gene52817:CDS |
| GACATATGTAACAAAGCTCA+AGG | 0.571855 | 3.1:+90686940 | MS.gene52817:CDS |
| CCTCTAACCTCTTAACCATA+CGG | 0.573081 | 3.1:-90687686 | None:intergenic |
| AATTTGTTCTGAATGCATTG+AGG | 0.584357 | 3.1:-90686909 | None:intergenic |
| TGAAGAAGAAGAGATCAAAG+TGG | 0.592386 | 3.1:+90687565 | MS.gene52817:CDS |
| TTTGGATACTATGAGCCGTA+TGG | 0.601069 | 3.1:+90687671 | MS.gene52817:CDS |
| ATTATGGTCATGGTAATGGA+AGG | 0.610111 | 3.1:+90687754 | MS.gene52817:CDS |
| AATATTGAAGATGGTTCTGA+GGG | 0.614569 | 3.1:+90687782 | MS.gene52817:CDS |
| AAGGTGTGTAATATTGAAGA+TGG | 0.621343 | 3.1:+90687773 | MS.gene52817:CDS |
| ATCATCGAAAGAACGTGATG+TGG | 0.634822 | 3.1:+90686846 | MS.gene52817:CDS |
| GGATCCACCAACTACATAAG+GGG | 0.639730 | 3.1:-90687532 | None:intergenic |
| TAATATTGAAGATGGTTCTG+AGG | 0.641414 | 3.1:+90687781 | MS.gene52817:CDS |
| TGTGTGAAGAATTATGGTCA+TGG | 0.650409 | 3.1:+90687744 | MS.gene52817:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACTAATTAATTAATTACT+TGG | - | chr3.1:90686754-90686773 | None:intergenic | 10.0% |
| !! | TTATATAGTGATAAATGATT+TGG | + | chr3.1:90687653-90687672 | MS.gene52817:CDS | 15.0% |
| !! | GAAGAAAAATAACAATAAAG+AGG | + | chr3.1:90687406-90687425 | MS.gene52817:CDS | 20.0% |
| !! | TTATTAATAAAAAAACCTGC+AGG | + | chr3.1:90686817-90686836 | MS.gene52817:intron | 20.0% |
| ! | AAAAATGATCAAGAGCTTTA+AGG | + | chr3.1:90687059-90687078 | MS.gene52817:CDS | 25.0% |
| ! | GATATTTGTGTGAAGAATTA+TGG | + | chr3.1:90687738-90687757 | MS.gene52817:CDS | 25.0% |
| ! | TTTGTAACAATTTCTTGTCT+AGG | - | chr3.1:90687042-90687061 | None:intergenic | 25.0% |
| !! | TGTCATATTCAACAACTTTT+TGG | - | chr3.1:90687215-90687234 | None:intergenic | 25.0% |
| AAATTGATGTTGCTGCAAAA+GGG | + | chr3.1:90687628-90687647 | MS.gene52817:CDS | 30.0% | |
| AACTAACTTTATGGATCAGT+TGG | + | chr3.1:90687821-90687840 | MS.gene52817:CDS | 30.0% | |
| AAGAATTATGGTCATGGTAA+TGG | + | chr3.1:90687750-90687769 | MS.gene52817:CDS | 30.0% | |
| AAGGTGTGTAATATTGAAGA+TGG | + | chr3.1:90687773-90687792 | MS.gene52817:CDS | 30.0% | |
| ACTGATCCATAAAGTTAGTT+TGG | - | chr3.1:90687821-90687840 | None:intergenic | 30.0% | |
| CGTCAAACTAGATTTGATTA+TGG | + | chr3.1:90687159-90687178 | MS.gene52817:CDS | 30.0% | |
| GCGGAAGTTAATAACATTAT+TGG | - | chr3.1:90687325-90687344 | None:intergenic | 30.0% | |
| TGATTGCCAAACTAACTTTA+TGG | + | chr3.1:90687812-90687831 | MS.gene52817:CDS | 30.0% | |
| TTAATAAAAAAACCTGCAGG+TGG | + | chr3.1:90686820-90686839 | MS.gene52817:intron | 30.0% | |
| TTGCTTTCCATAGCATAATT+GGG | + | chr3.1:90687499-90687518 | MS.gene52817:CDS | 30.0% | |
| TTTGCTTTCCATAGCATAAT+TGG | + | chr3.1:90687498-90687517 | MS.gene52817:CDS | 30.0% | |
| ! | AATTTGTTCTGAATGCATTG+AGG | - | chr3.1:90686912-90686931 | None:intergenic | 30.0% |
| ! | TAATATTGAAGATGGTTCTG+AGG | + | chr3.1:90687781-90687800 | MS.gene52817:CDS | 30.0% |
| ! | TGTGTTTTTCTTGAATATCG+CGG | - | chr3.1:90687344-90687363 | None:intergenic | 30.0% |
| !! | AATATTGAAGATGGTTCTGA+GGG | + | chr3.1:90687782-90687801 | MS.gene52817:CDS | 30.0% |
| AAGAGATCAAAGTGGGAAAA+TGG | + | chr3.1:90687573-90687592 | MS.gene52817:CDS | 35.0% | |
| AAGAGGTTATGCAAGAAAGT+TGG | + | chr3.1:90687423-90687442 | MS.gene52817:CDS | 35.0% | |
| AATTGATGTTGCTGCAAAAG+GGG | + | chr3.1:90687629-90687648 | MS.gene52817:CDS | 35.0% | |
| CAAATTGATGTTGCTGCAAA+AGG | + | chr3.1:90687627-90687646 | MS.gene52817:CDS | 35.0% | |
| CTTCTTCTTCATCAAACCAA+AGG | - | chr3.1:90687556-90687575 | None:intergenic | 35.0% | |
| GAAGAAGAAGAGATCAAAGT+GGG | + | chr3.1:90687566-90687585 | MS.gene52817:CDS | 35.0% | |
| GAATTGAACTCCAATATCAG+AGG | + | chr3.1:90686714-90686733 | MS.gene52817:intron | 35.0% | |
| GACATATGTAACAAAGCTCA+AGG | + | chr3.1:90686940-90686959 | MS.gene52817:CDS | 35.0% | |
| TAACAAAGCCACCAAAAAAG+CGG | - | chr3.1:90687475-90687494 | None:intergenic | 35.0% | |
| TGAAGAAGAAGAGATCAAAG+TGG | + | chr3.1:90687565-90687584 | MS.gene52817:CDS | 35.0% | |
| TGTAACAAAGCTCAAGGATA+TGG | + | chr3.1:90686946-90686965 | MS.gene52817:CDS | 35.0% | |
| TGTGTGAAGAATTATGGTCA+TGG | + | chr3.1:90687744-90687763 | MS.gene52817:CDS | 35.0% | |
| TTCTTTCTTCCGAAGAAGAT+CGG | + | chr3.1:90686593-90686612 | MS.gene52817:CDS | 35.0% | |
| !! | ATTATGGTCATGGTAATGGA+AGG | + | chr3.1:90687754-90687773 | MS.gene52817:CDS | 35.0% |
| AAGGATCCACCAACTACATA+AGG | - | chr3.1:90687537-90687556 | None:intergenic | 40.0% | |
| ACAAAGACATGCTTCTAAGC+TGG | - | chr3.1:90687115-90687134 | None:intergenic | 40.0% | |
| AGGATCCACCAACTACATAA+GGG | - | chr3.1:90687536-90687555 | None:intergenic | 40.0% | |
| AGGTTATGCAAGAAAGTTGG+AGG | + | chr3.1:90687426-90687445 | MS.gene52817:CDS | 40.0% | |
| ATAAAAAAACCTGCAGGTGG+TGG | + | chr3.1:90686823-90686842 | MS.gene52817:intron | 40.0% | |
| ATCATCGAAAGAACGTGATG+TGG | + | chr3.1:90686846-90686865 | MS.gene52817:CDS | 40.0% | |
| CAGCTAACCCAATTATGCTA+TGG | - | chr3.1:90687509-90687528 | None:intergenic | 40.0% | |
| CCTCTAACCTCTTAACCATA+CGG | - | chr3.1:90687689-90687708 | None:intergenic | 40.0% | |
| TAAGGTGCATCATCTTCTAG+TGG | + | chr3.1:90687077-90687096 | MS.gene52817:CDS | 40.0% | |
| TAGAGGATGAGGTTGAACAT+TGG | + | chr3.1:90687703-90687722 | MS.gene52817:CDS | 40.0% | |
| TAGTTTGACGAACACCTTGA+AGG | - | chr3.1:90687149-90687168 | None:intergenic | 40.0% | |
| TCTTTCTTCCGAAGAAGATC+GGG | + | chr3.1:90686594-90686613 | MS.gene52817:CDS | 40.0% | |
| TGAAGAAGAAGAAGTCGAAC+AGG | - | chr3.1:90687010-90687029 | None:intergenic | 40.0% | |
| TGCAAGAAAGTTGGAGGAAT+CGG | + | chr3.1:90687432-90687451 | MS.gene52817:CDS | 40.0% | |
| TGTTGCGACAAAATCCTTCA+AGG | + | chr3.1:90687132-90687151 | MS.gene52817:CDS | 40.0% | |
| TTAACTCACCCGATCTTCTT+CGG | - | chr3.1:90686605-90686624 | None:intergenic | 40.0% | |
| TTGAACTCCAATATCAGAGG+TGG | + | chr3.1:90686717-90686736 | MS.gene52817:intron | 40.0% | |
| TTTGGATACTATGAGCCGTA+TGG | + | chr3.1:90687671-90687690 | MS.gene52817:CDS | 40.0% | |
| !! | TATGTAGTTGGTGGATCCTT+TGG | + | chr3.1:90687537-90687556 | MS.gene52817:CDS | 40.0% |
| !!! | CGTTTCAGAAACCGCTTTTT+TGG | + | chr3.1:90687461-90687480 | MS.gene52817:CDS | 40.0% |
| !!! | TTCAGAAACCGCTTTTTTGG+TGG | + | chr3.1:90687464-90687483 | MS.gene52817:CDS | 40.0% |
| AAGCACACCACCTCTGATAT+TGG | - | chr3.1:90686727-90686746 | None:intergenic | 45.0% | |
| AATATCAGAGGTGGTGTGCT+TGG | + | chr3.1:90686726-90686745 | MS.gene52817:intron | 45.0% | |
| CCGTATGGTTAAGAGGTTAG+AGG | + | chr3.1:90687686-90687705 | MS.gene52817:CDS | 45.0% | |
| CTATGAGCCGTATGGTTAAG+AGG | + | chr3.1:90687679-90687698 | MS.gene52817:CDS | 45.0% | |
| GGATCCACCAACTACATAAG+GGG | - | chr3.1:90687535-90687554 | None:intergenic | 45.0% | |
| GGTTAAGAGGTTAGAGGATG+AGG | + | chr3.1:90687692-90687711 | MS.gene52817:CDS | 45.0% | |
| TGAGGTTGAACATTGGAGAG+AGG | + | chr3.1:90687710-90687729 | MS.gene52817:CDS | 45.0% | |
| GGATCAGTTGGAAGAGCTTG+AGG | + | chr3.1:90687833-90687852 | MS.gene52817:CDS | 50.0% | |
| GCTGCCCCTTATGTAGTTGG+TGG | + | chr3.1:90687528-90687547 | MS.gene52817:CDS | 55.0% | |
| GCTGCTGCCCCTTATGTAGT+TGG | + | chr3.1:90687525-90687544 | MS.gene52817:CDS | 55.0% | |
| TTCGATGATCCACCACCTGC+AGG | - | chr3.1:90686835-90686854 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 90686579 | 90687935 | 90686579 | ID=MS.gene52817 |
| chr3.1 | mRNA | 90686579 | 90687935 | 90686579 | ID=MS.gene52817.t1;Parent=MS.gene52817 |
| chr3.1 | exon | 90686579 | 90686615 | 90686579 | ID=MS.gene52817.t1.exon1;Parent=MS.gene52817.t1 |
| chr3.1 | CDS | 90686579 | 90686615 | 90686579 | ID=cds.MS.gene52817.t1;Parent=MS.gene52817.t1 |
| chr3.1 | exon | 90686839 | 90687935 | 90686839 | ID=MS.gene52817.t1.exon2;Parent=MS.gene52817.t1 |
| chr3.1 | CDS | 90686839 | 90687935 | 90686839 | ID=cds.MS.gene52817.t1;Parent=MS.gene52817.t1 |
| Gene Sequence |
| Protein sequence |
