Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52985.t1 | XP_027366903.1 | 91.7 | 96 | 8 | 0 | 1 | 96 | 63 | 158 | 3.30E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52985.t1 | Q9ZS51 | 62.1 | 95 | 36 | 0 | 1 | 95 | 77 | 171 | 1.3e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52985.t1 | G7I4T1 | 93.8 | 96 | 6 | 0 | 1 | 96 | 75 | 170 | 4.1e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene06077 | MS.gene52985 | -0.802181 | 6.34E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52985.t1 | MTR_1g075540 | 93.750 | 96 | 6 | 0 | 1 | 96 | 75 | 170 | 5.92e-65 | 194 |
| MS.gene52985.t1 | MTR_1g075540 | 93.684 | 95 | 6 | 0 | 1 | 95 | 75 | 169 | 7.06e-64 | 191 |
| MS.gene52985.t1 | MTR_2g023850 | 58.333 | 96 | 40 | 0 | 1 | 96 | 75 | 170 | 3.13e-41 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52985.t1 | AT4G14305 | 79.167 | 96 | 20 | 0 | 1 | 96 | 75 | 170 | 1.96e-56 | 172 |
| MS.gene52985.t1 | AT4G14305 | 79.167 | 96 | 20 | 0 | 1 | 96 | 63 | 158 | 2.60e-56 | 172 |
| MS.gene52985.t1 | AT4G04470 | 61.458 | 96 | 37 | 0 | 1 | 96 | 77 | 172 | 7.22e-43 | 138 |
| MS.gene52985.t1 | AT4G04470 | 61.458 | 96 | 37 | 0 | 1 | 96 | 77 | 172 | 7.22e-43 | 138 |
Find 16 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACCTTCCACGCCGTCAAC+TGG | 0.382299 | 1.1:+56504340 | None:intergenic |
| TATTATGGCTTGATTATTGA+AGG | 0.419845 | 1.1:-56505465 | MS.gene52985:intron |
| CAATGAAACTGTTGCTAAGA+AGG | 0.429878 | 1.1:-56505692 | MS.gene52985:intron |
| GGAACTGCAAAGGCATATAC+TGG | 0.477910 | 1.1:+56504194 | None:intergenic |
| CTATCCTACTGTCCAGTTGA+CGG | 0.478021 | 1.1:-56504352 | MS.gene52985:CDS |
| ACAGCTTTGTTGGTTCATGC+TGG | 0.506633 | 1.1:-56504159 | MS.gene52985:intron |
| GTTGGTTTGCAGGAAGACCT+TGG | 0.511703 | 1.1:-56504404 | MS.gene52985:intron |
| AGCAAATAACTAGTTCTCCA+TGG | 0.534969 | 1.1:-56505509 | MS.gene52985:CDS |
| GAGAACTAGTTATTTGCTCA+AGG | 0.536726 | 1.1:+56505514 | None:intergenic |
| TGTCCAGTTGACGGCGTGGA+AGG | 0.546675 | 1.1:-56504343 | MS.gene52985:intron |
| TTGTTCATGACTATATTCCA+AGG | 0.574186 | 1.1:+56504387 | None:intergenic |
| CACGCCGTCAACTGGACAGT+AGG | 0.614091 | 1.1:+56504348 | None:intergenic |
| TAATTCACCCAACCAACGAT+AGG | 0.618119 | 1.1:+56504217 | None:intergenic |
| AATAAAACACGGAACTGCAA+AGG | 0.621872 | 1.1:+56504184 | None:intergenic |
| CTACTGTCCAGTTGACGGCG+TGG | 0.646340 | 1.1:-56504347 | MS.gene52985:CDS |
| CAAAGCTGTGAAATAAAACA+CGG | 0.671747 | 1.1:+56504173 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATGAATAAGTATCAT+TGG | - | chr1.1:56505161-56505180 | MS.gene52985:intron | 15.0% |
| !! | AAGAGTTAAAATACATAAAT+AGG | - | chr1.1:56505425-56505444 | MS.gene52985:intron | 15.0% |
| !! | ACTCAAAATAATATTCATTT+CGG | - | chr1.1:56505649-56505668 | MS.gene52985:intron | 15.0% |
| !! | ATAATGATTATTGTAAACTT+AGG | + | chr1.1:56504105-56504124 | None:intergenic | 15.0% |
| !! | ATGATTAAGATATTATCAAT+AGG | + | chr1.1:56504713-56504732 | None:intergenic | 15.0% |
| !! | CAATTAATACTAAATATTAG+AGG | + | chr1.1:56503967-56503986 | None:intergenic | 15.0% |
| !! | GAAAACATTTATAGAATAAA+AGG | - | chr1.1:56504259-56504278 | MS.gene52985:intron | 15.0% |
| !! | TAATGATTATTGTAAACTTA+GGG | + | chr1.1:56504104-56504123 | None:intergenic | 15.0% |
| !! | TCAAAATTATCAATGTTTAT+AGG | - | chr1.1:56504067-56504086 | MS.gene52985:intron | 15.0% |
| !! | TGTTTAAATGAAAATCTTAT+TGG | + | chr1.1:56505406-56505425 | None:intergenic | 15.0% |
| !!! | ATGATACTTATTCATTATTT+TGG | + | chr1.1:56505161-56505180 | None:intergenic | 15.0% |
| !! | AATTTAAGAAAGTAAGGATT+TGG | + | chr1.1:56504338-56504357 | None:intergenic | 20.0% |
| !! | AGATTACAAAAGGTTATATT+CGG | - | chr1.1:56505220-56505239 | MS.gene52985:intron | 20.0% |
| !! | GAATGAAATATCATATTACT+AGG | - | chr1.1:56503679-56503698 | MS.gene52985:intron | 20.0% |
| !! | GCAATTAAAATTCAAATTGA+GGG | + | chr1.1:56505566-56505585 | None:intergenic | 20.0% |
| !! | TCATTATTTACAAGTCAATA+TGG | - | chr1.1:56504118-56504137 | MS.gene52985:intron | 20.0% |
| !! | TGCAATTAAAATTCAAATTG+AGG | + | chr1.1:56505567-56505586 | None:intergenic | 20.0% |
| !! | TTAACATTGAATTTAATTCC+CGG | - | chr1.1:56504376-56504395 | MS.gene52985:CDS | 20.0% |
| !!! | ACATTGATAATTTTGAACTT+CGG | + | chr1.1:56504063-56504082 | None:intergenic | 20.0% |
| !!! | CAATTTGAATAGTTAGTTTT+TGG | - | chr1.1:56504941-56504960 | MS.gene52985:intron | 20.0% |
| !!! | CATAAATAGGTTTTTAATGA+AGG | - | chr1.1:56505438-56505457 | MS.gene52985:intron | 20.0% |
| !!! | GAATATTATTTTGAGTATGA+TGG | + | chr1.1:56505645-56505664 | None:intergenic | 20.0% |
| !!! | TCACAATTTTTACTTAATCA+AGG | + | chr1.1:56505506-56505525 | None:intergenic | 20.0% |
| !!! | TTTTTGTTCATGATGTATTA+TGG | - | chr1.1:56503740-56503759 | MS.gene52985:intron | 20.0% |
| ! | AACTATTCAAATTGTTCCAT+TGG | + | chr1.1:56504935-56504954 | None:intergenic | 25.0% |
| ! | AATGAATAAGTATCATTGGT+TGG | - | chr1.1:56505165-56505184 | MS.gene52985:intron | 25.0% |
| ! | CAGAACAATTTAAGAAAGTA+AGG | + | chr1.1:56504344-56504363 | None:intergenic | 25.0% |
| ! | GCAATATCATAATTAAAAGC+AGG | + | chr1.1:56505360-56505379 | None:intergenic | 25.0% |
| ! | TATTATGGCTTGATTATTGA+AGG | - | chr1.1:56503755-56503774 | MS.gene52985:intron | 25.0% |
| ! | TGAAACATGTACATGAATAA+AGG | - | chr1.1:56503846-56503865 | MS.gene52985:intron | 25.0% |
| ! | TTAAGATATTATCAATAGGC+TGG | + | chr1.1:56504709-56504728 | None:intergenic | 25.0% |
| ! | TTACCTTACAAGTATATGAA+GGG | - | chr1.1:56504304-56504323 | MS.gene52985:intron | 25.0% |
| ! | TTGATTAAACTATACAAGAG+AGG | + | chr1.1:56504589-56504608 | None:intergenic | 25.0% |
| ! | TTGTTCACAATCTCATATTA+AGG | + | chr1.1:56504232-56504251 | None:intergenic | 25.0% |
| !! | TAGATGAAGTTTAAGCTTTT+TGG | - | chr1.1:56504626-56504645 | MS.gene52985:intron | 25.0% |
| !! | TGTAACTTGTAAAGTTGTAA+TGG | - | chr1.1:56503886-56503905 | MS.gene52985:intron | 25.0% |
| !! | TTTTCATAAAACAAGTACCA+GGG | + | chr1.1:56503651-56503670 | None:intergenic | 25.0% |
| !! | TTTTTCATAAAACAAGTACC+AGG | + | chr1.1:56503652-56503671 | None:intergenic | 25.0% |
| !!! | TGAATTTTAATTGCACCATA+AGG | - | chr1.1:56505572-56505591 | MS.gene52985:intron | 25.0% |
| !!! | TGTATAGTTGTACTGATTTA+TGG | - | chr1.1:56505685-56505704 | MS.gene52985:intron | 25.0% |
| !!! | TTGAATAGTTAGTTTTTGGA+AGG | - | chr1.1:56504945-56504964 | MS.gene52985:intron | 25.0% |
| AAAATTGTGAAGATGCAATG+TGG | - | chr1.1:56505516-56505535 | MS.gene52985:CDS | 30.0% | |
| AACCAAACTGAAATAGTGAA+GGG | - | chr1.1:56504515-56504534 | MS.gene52985:intron | 30.0% | |
| ATGAACAAAAAGTTGTTCCA+TGG | + | chr1.1:56503731-56503750 | None:intergenic | 30.0% | |
| CAAAGCTGTGAAATAAAACA+CGG | + | chr1.1:56505050-56505069 | None:intergenic | 30.0% | |
| CATATTTACTCTATAGAAGC+AGG | - | chr1.1:56505251-56505270 | MS.gene52985:intron | 30.0% | |
| CATGCAGTATAGATTACAAA+AGG | - | chr1.1:56505210-56505229 | MS.gene52985:intron | 30.0% | |
| CTATTTATCCCTATAACCAA+AGG | + | chr1.1:56503589-56503608 | None:intergenic | 30.0% | |
| CTGCTTCTATAGAGTAAATA+TGG | + | chr1.1:56505253-56505272 | None:intergenic | 30.0% | |
| CTTACCTTACAAGTATATGA+AGG | - | chr1.1:56504303-56504322 | MS.gene52985:intron | 30.0% | |
| GATAGTGTCAATACATCATT+AGG | - | chr1.1:56505380-56505399 | MS.gene52985:intron | 30.0% | |
| GTCATAAATCTTCTTCTGTT+TGG | - | chr1.1:56504909-56504928 | MS.gene52985:intron | 30.0% | |
| TACTATGTCCTTTGGTTATA+GGG | - | chr1.1:56503578-56503597 | MS.gene52985:intron | 30.0% | |
| TCAAAATTAAAGTGTGAGGT+TGG | - | chr1.1:56504798-56504817 | MS.gene52985:intron | 30.0% | |
| TGCTTCAAAATTAAAGTGTG+AGG | - | chr1.1:56504794-56504813 | MS.gene52985:intron | 30.0% | |
| TTCTGCTTAATTCCTAACTT+TGG | - | chr1.1:56503995-56504014 | MS.gene52985:intron | 30.0% | |
| TTGTTCATGACTATATTCCA+AGG | + | chr1.1:56504836-56504855 | None:intergenic | 30.0% | |
| ! | AAAAGTTTTGCACCGAAATT+TGG | + | chr1.1:56504191-56504210 | None:intergenic | 30.0% |
| ! | AAAGTTTTGCACCGAAATTT+GGG | + | chr1.1:56504190-56504209 | None:intergenic | 30.0% |
| ! | GAATGTCAATTCTGTTTTCT+TGG | + | chr1.1:56503945-56503964 | None:intergenic | 30.0% |
| ! | TTAATTCCTAACTTTGGTTC+TGG | - | chr1.1:56504001-56504020 | MS.gene52985:intron | 30.0% |
| !!! | ATTTTAATTGCACCATAAGG+TGG | - | chr1.1:56505575-56505594 | MS.gene52985:intron | 30.0% |
| !!! | GTTTTATTTCACAGCTTTGT+TGG | - | chr1.1:56505051-56505070 | MS.gene52985:intron | 30.0% |
| !!! | GTTTTTTGAACATGCAGTTT+TGG | - | chr1.1:56504980-56504999 | MS.gene52985:intron | 30.0% |
| AAAGCAGGACTCGATAAAAA+AGG | + | chr1.1:56505345-56505364 | None:intergenic | 35.0% | |
| AATAAAACACGGAACTGCAA+AGG | + | chr1.1:56505039-56505058 | None:intergenic | 35.0% | |
| ACATTGAATTTAATTCCCGG+AGG | - | chr1.1:56504379-56504398 | MS.gene52985:CDS | 35.0% | |
| AGACCCTTCATATACTTGTA+AGG | + | chr1.1:56504310-56504329 | None:intergenic | 35.0% | |
| AGACCTTCTATCCCAAATTT+CGG | - | chr1.1:56504176-56504195 | MS.gene52985:CDS | 35.0% | |
| ATAGTACCAGAACCAAAGTT+AGG | + | chr1.1:56504010-56504029 | None:intergenic | 35.0% | |
| ATTGTGTGTACTATGTCCTT+TGG | - | chr1.1:56503570-56503589 | MS.gene52985:intron | 35.0% | |
| CAATGAAACTGTTGCTAAGA+AGG | - | chr1.1:56503528-56503547 | MS.gene52985:intron | 35.0% | |
| CAGTACAACTATACACGATT+TGG | + | chr1.1:56505680-56505699 | None:intergenic | 35.0% | |
| GAACCAAACTGAAATAGTGA+AGG | - | chr1.1:56504514-56504533 | MS.gene52985:intron | 35.0% | |
| GAGAACTAGTTATTTGCTCA+AGG | + | chr1.1:56503709-56503728 | None:intergenic | 35.0% | |
| GTACTATGTCCTTTGGTTAT+AGG | - | chr1.1:56503577-56503596 | MS.gene52985:intron | 35.0% | |
| GTTCATAGTACTGTGAGTAT+AGG | - | chr1.1:56505187-56505206 | MS.gene52985:intron | 35.0% | |
| TGATTTATGGTCATTGTCCA+GGG | - | chr1.1:56505698-56505717 | MS.gene52985:CDS | 35.0% | |
| ! | ACAATGCACACTGATTGATT+TGG | - | chr1.1:56505467-56505486 | MS.gene52985:CDS | 35.0% |
| ! | AGCAAATAACTAGTTCTCCA+TGG | - | chr1.1:56503711-56503730 | MS.gene52985:intron | 35.0% |
| ! | ATTCTGTTTTCTTGGTAGAG+AGG | + | chr1.1:56503937-56503956 | None:intergenic | 35.0% |
| ! | GTATTTTGTGTTGTGTGATC+AGG | - | chr1.1:56503608-56503627 | MS.gene52985:intron | 35.0% |
| ! | TGAGTATGATGGAATAGTCT+CGG | + | chr1.1:56505634-56505653 | None:intergenic | 35.0% |
| ! | TTTGAAGCACTATGCAATGA+AGG | + | chr1.1:56504783-56504802 | None:intergenic | 35.0% |
| !! | AAACTTTTCAAGGGGAAGAA+AGG | - | chr1.1:56503505-56503524 | MS.gene52985:intron | 35.0% |
| ATAACCAGAGAACCACCTTA+TGG | + | chr1.1:56505590-56505609 | None:intergenic | 40.0% | |
| ATCTTCTTCTGTTTGGCCAA+TGG | - | chr1.1:56504916-56504935 | MS.gene52985:intron | 40.0% | |
| CTGATTTATGGTCATTGTCC+AGG | - | chr1.1:56505697-56505716 | MS.gene52985:CDS | 40.0% | |
| GACCCTTCACTATTTCAGTT+TGG | + | chr1.1:56504520-56504539 | None:intergenic | 40.0% | |
| TAAAACAAGTACCAGGGCTA+AGG | + | chr1.1:56503645-56503664 | None:intergenic | 40.0% | |
| TAATTCACCCAACCAACGAT+AGG | + | chr1.1:56505006-56505025 | None:intergenic | 40.0% | |
| TAGGCTGGAATTGTTTCATG+AGG | + | chr1.1:56504694-56504713 | None:intergenic | 40.0% | |
| TTCCTTATATTGCTCTCCTC+CGG | + | chr1.1:56504398-56504417 | None:intergenic | 40.0% | |
| ! | TGTTTTCTTGGTAGAGAGGT+GGG | + | chr1.1:56503933-56503952 | None:intergenic | 40.0% |
| ! | TTCTATGTTTTCCTTAGCCC+TGG | - | chr1.1:56503631-56503650 | MS.gene52985:intron | 40.0% |
| !!! | AGGCTCAGTTTTGACTTAAC+AGG | - | chr1.1:56505271-56505290 | MS.gene52985:intron | 40.0% |
| !!! | CAGTTTTGACTTAACAGGAG+AGG | - | chr1.1:56505276-56505295 | MS.gene52985:intron | 40.0% |
| CTATCCTACTGTCCAGTTGA+CGG | - | chr1.1:56504868-56504887 | MS.gene52985:intron | 45.0% | |
| TCCTTATATTGCTCTCCTCC+GGG | + | chr1.1:56504397-56504416 | None:intergenic | 45.0% | |
| ! | CTGTTTTCTTGGTAGAGAGG+TGG | + | chr1.1:56503934-56503953 | None:intergenic | 45.0% |
| ! | GCACCGAAATTTGGGATAGA+AGG | + | chr1.1:56504182-56504201 | None:intergenic | 45.0% |
| ! | GGAACTGCAAAGGCATATAC+TGG | + | chr1.1:56505029-56505048 | None:intergenic | 45.0% |
| !! | AAAGTGTGAGGTTGGTTTGC+AGG | - | chr1.1:56504806-56504825 | MS.gene52985:intron | 45.0% |
| !! | ACAGCTTTGTTGGTTCATGC+TGG | - | chr1.1:56505061-56505080 | MS.gene52985:intron | 45.0% |
| !! | AGTTTTGGCCTATCGTTGGT+TGG | - | chr1.1:56504995-56505014 | MS.gene52985:intron | 45.0% |
| !! | ATGCAGTTTTGGCCTATCGT+TGG | - | chr1.1:56504991-56505010 | MS.gene52985:intron | 45.0% |
| !! | GTTTTGGCCTATCGTTGGTT+GGG | - | chr1.1:56504996-56505015 | MS.gene52985:intron | 45.0% |
| GAGAATATCACGATGAGCCC+TGG | + | chr1.1:56505718-56505737 | None:intergenic | 50.0% | |
| TCCCGGAGGAGAGCAATATA+AGG | - | chr1.1:56504393-56504412 | MS.gene52985:CDS | 50.0% | |
| TGCACCATAAGGTGGTTCTC+TGG | - | chr1.1:56505583-56505602 | MS.gene52985:intron | 50.0% | |
| !! | GGTGGGTTACACAACACAAG+AGG | + | chr1.1:56503916-56503935 | None:intergenic | 50.0% |
| !! | GTTGGTTTGCAGGAAGACCT+TGG | - | chr1.1:56504816-56504835 | MS.gene52985:intron | 50.0% |
| ATACCTTCCACGCCGTCAAC+TGG | + | chr1.1:56504883-56504902 | None:intergenic | 55.0% | |
| CACGCCGTCAACTGGACAGT+AGG | + | chr1.1:56504875-56504894 | None:intergenic | 60.0% | |
| CTACTGTCCAGTTGACGGCG+TGG | - | chr1.1:56504873-56504892 | MS.gene52985:intron | 60.0% | |
| TGTCCAGTTGACGGCGTGGA+AGG | - | chr1.1:56504877-56504896 | MS.gene52985:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 56503499 | 56505743 | 56503499 | ID=MS.gene52985 |
| chr1.1 | mRNA | 56503499 | 56505743 | 56503499 | ID=MS.gene52985.t1;Parent=MS.gene52985 |
| chr1.1 | exon | 56505693 | 56505743 | 56505693 | ID=MS.gene52985.t1.exon1;Parent=MS.gene52985.t1 |
| chr1.1 | CDS | 56505693 | 56505743 | 56505693 | ID=cds.MS.gene52985.t1;Parent=MS.gene52985.t1 |
| chr1.1 | exon | 56505466 | 56505541 | 56505466 | ID=MS.gene52985.t1.exon2;Parent=MS.gene52985.t1 |
| chr1.1 | CDS | 56505466 | 56505541 | 56505466 | ID=cds.MS.gene52985.t1;Parent=MS.gene52985.t1 |
| chr1.1 | exon | 56504344 | 56504414 | 56504344 | ID=MS.gene52985.t1.exon3;Parent=MS.gene52985.t1 |
| chr1.1 | CDS | 56504344 | 56504414 | 56504344 | ID=cds.MS.gene52985.t1;Parent=MS.gene52985.t1 |
| chr1.1 | exon | 56504160 | 56504245 | 56504160 | ID=MS.gene52985.t1.exon4;Parent=MS.gene52985.t1 |
| chr1.1 | CDS | 56504160 | 56504245 | 56504160 | ID=cds.MS.gene52985.t1;Parent=MS.gene52985.t1 |
| chr1.1 | exon | 56503499 | 56503523 | 56503499 | ID=MS.gene52985.t1.exon5;Parent=MS.gene52985.t1 |
| chr1.1 | CDS | 56503499 | 56503523 | 56503499 | ID=cds.MS.gene52985.t1;Parent=MS.gene52985.t1 |
| Gene Sequence |
| Protein sequence |
