Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53086.t1 | XP_004490115.1 | 85.5 | 110 | 15 | 1 | 1 | 110 | 1 | 109 | 1.60E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53086.t1 | A0A1S2XJ41 | 85.5 | 110 | 15 | 1 | 1 | 110 | 1 | 109 | 1.1e-43 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059545 | MS.gene53086 | -0.816854 | 4.46E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53086.t1 | MTR_5g042520 | 85.714 | 112 | 14 | 1 | 1 | 110 | 1 | 112 | 1.23e-60 | 187 |
| MS.gene53086.t1 | MTR_5g042520 | 85.714 | 112 | 14 | 1 | 1 | 110 | 1 | 112 | 2.49e-60 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53086.t1 | AT1G13990 | 47.287 | 129 | 47 | 5 | 1 | 110 | 1 | 127 | 1.62e-19 | 80.9 |
| MS.gene53086.t1 | AT1G13990 | 47.287 | 129 | 47 | 5 | 1 | 110 | 1 | 127 | 2.47e-19 | 80.9 |
| MS.gene53086.t1 | AT1G13990 | 47.287 | 129 | 47 | 5 | 1 | 110 | 1 | 127 | 2.79e-19 | 80.9 |
Find 47 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACTCATCCGAGCTTCC+AGG | 0.336242 | 5.1:-31995502 | None:intergenic |
| CGACGGAAGGGGTTGGTGGG+AGG | 0.341218 | 5.1:-31995307 | None:intergenic |
| GTGGCGACGGAAGGGGTTGG+TGG | 0.346987 | 5.1:-31995311 | None:intergenic |
| CATATGGATGGTAAAATTCC+TGG | 0.374107 | 5.1:+31995484 | MS.gene53086:CDS |
| GTTGGTGGGAGGGAGGACTA+TGG | 0.393546 | 5.1:-31995296 | None:intergenic |
| GGCGGGTGTGGCGACGGAAG+GGG | 0.401705 | 5.1:-31995318 | None:intergenic |
| AGAGAGAGAGGCGGCGGGTG+TGG | 0.404308 | 5.1:-31995330 | None:intergenic |
| GCGGCGGGTGTGGCGACGGA+AGG | 0.409468 | 5.1:-31995320 | None:intergenic |
| CTTCGTTCCTCCTCCTGGTT+CGG | 0.428630 | 5.1:-31995396 | None:intergenic |
| CGATACTTCGTTCCTCCTCC+TGG | 0.435769 | 5.1:-31995401 | None:intergenic |
| AGCAGCGGTGGAGGAGAGGA+CGG | 0.456583 | 5.1:+31995433 | MS.gene53086:CDS |
| GGAAGGGGTTGGTGGGAGGG+AGG | 0.458182 | 5.1:-31995303 | None:intergenic |
| GACGGAAGGGGTTGGTGGGA+GGG | 0.458747 | 5.1:-31995306 | None:intergenic |
| GGTGGAGAAAGCGCTTCATA+TGG | 0.468825 | 5.1:+31995468 | MS.gene53086:CDS |
| TTTGATTCTAGCAGCTATGA+TGG | 0.481586 | 5.1:+31995577 | MS.gene53086:CDS |
| GGAGAAGAGAGAGAGGCGGC+GGG | 0.484370 | 5.1:-31995335 | None:intergenic |
| CGGCGGGTGTGGCGACGGAA+GGG | 0.485102 | 5.1:-31995319 | None:intergenic |
| TGCAAAGTCAGCAGCGGTGG+AGG | 0.488867 | 5.1:+31995424 | MS.gene53086:CDS |
| GGGAGAAGAGAGAGAGGCGG+CGG | 0.493916 | 5.1:-31995336 | None:intergenic |
| AGAGGCGGCGGGTGTGGCGA+CGG | 0.499779 | 5.1:-31995324 | None:intergenic |
| AGAAGAGTCTCGGAGGAAGA+TGG | 0.501382 | 5.1:-31995360 | None:intergenic |
| GCAGCGGTGGAGGAGAGGAC+GGG | 0.502707 | 5.1:+31995434 | MS.gene53086:CDS |
| AGAGGACGGGAGAGATGAAG+AGG | 0.520462 | 5.1:+31995447 | MS.gene53086:CDS |
| CTACACATCGCGTCCGAACC+AGG | 0.520972 | 5.1:+31995383 | MS.gene53086:CDS |
| AGTCAGCAGCGGTGGAGGAG+AGG | 0.526025 | 5.1:+31995429 | MS.gene53086:CDS |
| GGTGTGGCGACGGAAGGGGT+TGG | 0.531215 | 5.1:-31995314 | None:intergenic |
| GGACGGGAGAGATGAAGAGG+TGG | 0.531364 | 5.1:+31995450 | MS.gene53086:CDS |
| TGGCGACGGAAGGGGTTGGT+GGG | 0.533423 | 5.1:-31995310 | None:intergenic |
| GAGTCTCGGAGGAAGATGGC+GGG | 0.543744 | 5.1:-31995356 | None:intergenic |
| ACTATGGAAGGTGATATTGA+AGG | 0.552945 | 5.1:-31995280 | None:intergenic |
| ATCATAGCTGCTAGAATCAA+AGG | 0.555592 | 5.1:-31995575 | None:intergenic |
| TGGTAAAATTCCTGGAAGCT+CGG | 0.563150 | 5.1:+31995492 | MS.gene53086:CDS |
| TCGTGCAAAGTCAGCAGCGG+TGG | 0.577748 | 5.1:+31995421 | MS.gene53086:CDS |
| GATGGCGGGAGAAGAGAGAG+AGG | 0.579533 | 5.1:-31995342 | None:intergenic |
| ATCGCGTCCGAACCAGGAGG+AGG | 0.582438 | 5.1:+31995389 | MS.gene53086:CDS |
| AGAGTCTCGGAGGAAGATGG+CGG | 0.608815 | 5.1:-31995357 | None:intergenic |
| TCCTACGCATATCATAAGCA+CGG | 0.615988 | 5.1:-31995540 | None:intergenic |
| GGCGGGAGAAGAGAGAGAGG+CGG | 0.617937 | 5.1:-31995339 | None:intergenic |
| GAGAAAGCGCTTCATATGGA+TGG | 0.619677 | 5.1:+31995472 | MS.gene53086:CDS |
| CCTACGCATATCATAAGCAC+GGG | 0.621176 | 5.1:-31995539 | None:intergenic |
| CGATGTGTAGAGAAGAGTCT+CGG | 0.639811 | 5.1:-31995370 | None:intergenic |
| CCCGTGCTTATGATATGCGT+AGG | 0.646813 | 5.1:+31995539 | MS.gene53086:CDS |
| ACGCATATCATAAGCACGGG+AGG | 0.665509 | 5.1:-31995536 | None:intergenic |
| GTGGGAGGGAGGACTATGGA+AGG | 0.675207 | 5.1:-31995292 | None:intergenic |
| TGTGTAGAGAAGAGTCTCGG+AGG | 0.689941 | 5.1:-31995367 | None:intergenic |
| GTATCGTGCAAAGTCAGCAG+CGG | 0.717527 | 5.1:+31995418 | MS.gene53086:CDS |
| CACATCGCGTCCGAACCAGG+AGG | 0.764506 | 5.1:+31995386 | MS.gene53086:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTATGGAAGGTGATATTGA+AGG | - | chr5.1:31995283-31995302 | None:intergenic | 35.0% | |
| ATCATAGCTGCTAGAATCAA+AGG | - | chr5.1:31995578-31995597 | None:intergenic | 35.0% | |
| CATATGGATGGTAAAATTCC+TGG | + | chr5.1:31995484-31995503 | MS.gene53086:CDS | 35.0% | |
| TCCTACGCATATCATAAGCA+CGG | - | chr5.1:31995543-31995562 | None:intergenic | 40.0% | |
| TGGTAAAATTCCTGGAAGCT+CGG | + | chr5.1:31995492-31995511 | MS.gene53086:CDS | 40.0% | |
| CCTACGCATATCATAAGCAC+GGG | - | chr5.1:31995542-31995561 | None:intergenic | 45.0% | |
| CGATGTGTAGAGAAGAGTCT+CGG | - | chr5.1:31995373-31995392 | None:intergenic | 45.0% | |
| !! | GAGAAAGCGCTTCATATGGA+TGG | + | chr5.1:31995472-31995491 | MS.gene53086:CDS | 45.0% |
| AAGAACTCATCCGAGCTTCC+AGG | - | chr5.1:31995505-31995524 | None:intergenic | 50.0% | |
| ACGCATATCATAAGCACGGG+AGG | - | chr5.1:31995539-31995558 | None:intergenic | 50.0% | |
| AGAAGAGTCTCGGAGGAAGA+TGG | - | chr5.1:31995363-31995382 | None:intergenic | 50.0% | |
| CCCGTGCTTATGATATGCGT+AGG | + | chr5.1:31995539-31995558 | MS.gene53086:CDS | 50.0% | |
| GTATCGTGCAAAGTCAGCAG+CGG | + | chr5.1:31995418-31995437 | MS.gene53086:CDS | 50.0% | |
| TGTGTAGAGAAGAGTCTCGG+AGG | - | chr5.1:31995370-31995389 | None:intergenic | 50.0% | |
| !! | GGTGGAGAAAGCGCTTCATA+TGG | + | chr5.1:31995468-31995487 | MS.gene53086:CDS | 50.0% |
| AGAGGACGGGAGAGATGAAG+AGG | + | chr5.1:31995447-31995466 | MS.gene53086:CDS | 55.0% | |
| AGAGTCTCGGAGGAAGATGG+CGG | - | chr5.1:31995360-31995379 | None:intergenic | 55.0% | |
| CGATACTTCGTTCCTCCTCC+TGG | - | chr5.1:31995404-31995423 | None:intergenic | 55.0% | |
| CTTCGTTCCTCCTCCTGGTT+CGG | - | chr5.1:31995399-31995418 | None:intergenic | 55.0% | |
| CTACACATCGCGTCCGAACC+AGG | + | chr5.1:31995383-31995402 | MS.gene53086:CDS | 60.0% | |
| GAGTCTCGGAGGAAGATGGC+GGG | - | chr5.1:31995359-31995378 | None:intergenic | 60.0% | |
| GGACGGGAGAGATGAAGAGG+TGG | + | chr5.1:31995450-31995469 | MS.gene53086:CDS | 60.0% | |
| GTGGGAGGGAGGACTATGGA+AGG | - | chr5.1:31995295-31995314 | None:intergenic | 60.0% | |
| TCGTGCAAAGTCAGCAGCGG+TGG | + | chr5.1:31995421-31995440 | MS.gene53086:CDS | 60.0% | |
| TGCAAAGTCAGCAGCGGTGG+AGG | + | chr5.1:31995424-31995443 | MS.gene53086:CDS | 60.0% | |
| ! | GTTGGTGGGAGGGAGGACTA+TGG | - | chr5.1:31995299-31995318 | None:intergenic | 60.0% |
| !! | GATGGCGGGAGAAGAGAGAG+AGG | - | chr5.1:31995345-31995364 | None:intergenic | 60.0% |
| AGCAGCGGTGGAGGAGAGGA+CGG | + | chr5.1:31995433-31995452 | MS.gene53086:CDS | 65.0% | |
| AGTCAGCAGCGGTGGAGGAG+AGG | + | chr5.1:31995429-31995448 | MS.gene53086:CDS | 65.0% | |
| ATCGCGTCCGAACCAGGAGG+AGG | + | chr5.1:31995389-31995408 | MS.gene53086:CDS | 65.0% | |
| CACATCGCGTCCGAACCAGG+AGG | + | chr5.1:31995386-31995405 | MS.gene53086:CDS | 65.0% | |
| GGAGAAGAGAGAGAGGCGGC+GGG | - | chr5.1:31995338-31995357 | None:intergenic | 65.0% | |
| GGCGGGAGAAGAGAGAGAGG+CGG | - | chr5.1:31995342-31995361 | None:intergenic | 65.0% | |
| GGGAGAAGAGAGAGAGGCGG+CGG | - | chr5.1:31995339-31995358 | None:intergenic | 65.0% | |
| ! | TGGCGACGGAAGGGGTTGGT+GGG | - | chr5.1:31995313-31995332 | None:intergenic | 65.0% |
| !! | GACGGAAGGGGTTGGTGGGA+GGG | - | chr5.1:31995309-31995328 | None:intergenic | 65.0% |
| AGAGAGAGAGGCGGCGGGTG+TGG | - | chr5.1:31995333-31995352 | None:intergenic | 70.0% | |
| GCAGCGGTGGAGGAGAGGAC+GGG | + | chr5.1:31995434-31995453 | MS.gene53086:CDS | 70.0% | |
| GGTGTGGCGACGGAAGGGGT+TGG | - | chr5.1:31995317-31995336 | None:intergenic | 70.0% | |
| GTGGCGACGGAAGGGGTTGG+TGG | - | chr5.1:31995314-31995333 | None:intergenic | 70.0% | |
| !! | CGACGGAAGGGGTTGGTGGG+AGG | - | chr5.1:31995310-31995329 | None:intergenic | 70.0% |
| !! | GGAAGGGGTTGGTGGGAGGG+AGG | - | chr5.1:31995306-31995325 | None:intergenic | 70.0% |
| AGAGGCGGCGGGTGTGGCGA+CGG | - | chr5.1:31995327-31995346 | None:intergenic | 75.0% | |
| CGGCGGGTGTGGCGACGGAA+GGG | - | chr5.1:31995322-31995341 | None:intergenic | 75.0% | |
| GGCGGGTGTGGCGACGGAAG+GGG | - | chr5.1:31995321-31995340 | None:intergenic | 75.0% | |
| ! | GCGGCGGGTGTGGCGACGGA+AGG | - | chr5.1:31995323-31995342 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 31995268 | 31995598 | 31995268 | ID=MS.gene53086 |
| chr5.1 | mRNA | 31995268 | 31995598 | 31995268 | ID=MS.gene53086.t1;Parent=MS.gene53086 |
| chr5.1 | exon | 31995268 | 31995598 | 31995268 | ID=MS.gene53086.t1.exon1;Parent=MS.gene53086.t1 |
| chr5.1 | CDS | 31995268 | 31995598 | 31995268 | ID=cds.MS.gene53086.t1;Parent=MS.gene53086.t1 |
| Gene Sequence |
| Protein sequence |
