AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53254

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53254.t1 MTR_8g087470 95.570 316 13 1 1 315 1 316 0.0 525
MS.gene53254.t1 MTR_8g087460 93.987 316 18 1 1 315 1 316 0.0 518
MS.gene53254.t1 MTR_2g017950 60.248 322 83 9 4 312 2 291 3.34e-106 311
MS.gene53254.t1 MTR_4g059840 51.362 257 95 8 7 256 5 238 1.81e-69 218
MS.gene53254.t1 MTR_4g053380 50.752 266 104 10 6 265 6 250 1.11e-66 211
MS.gene53254.t1 MTR_4g059790 49.402 251 101 9 71 315 53 283 8.33e-66 209
MS.gene53254.t1 MTR_2g017970 59.563 183 65 5 93 274 68 242 1.77e-61 197
MS.gene53254.t1 MTR_2g017960 52.232 224 90 5 93 315 68 275 2.63e-61 196
MS.gene53254.t1 MTR_4g059720 62.011 179 58 5 67 244 21 190 3.30e-61 195
MS.gene53254.t1 MTR_2g093970 41.720 314 152 8 4 312 1 288 7.75e-61 196
MS.gene53254.t1 MTR_2g093980 45.878 279 123 8 5 281 1 253 8.73e-60 193
MS.gene53254.t1 MTR_2g093990 57.222 180 72 3 66 244 14 189 1.05e-57 186
MS.gene53254.t1 MTR_4g059780 57.921 202 68 8 42 242 52 237 2.65e-56 184
MS.gene53254.t1 MTR_8g087450 43.729 295 142 10 22 314 16 288 1.51e-55 182
MS.gene53254.t1 MTR_7g101080 51.266 158 70 4 87 242 35 187 5.17e-45 154
MS.gene53254.t1 MTR_2g090765 42.458 179 93 6 66 242 14 184 1.91e-29 113
MS.gene53254.t1 MTR_5g098060 31.977 172 113 2 92 263 47 214 1.67e-18 84.0
MS.gene53254.t1 MTR_7g075453 32.812 192 106 6 74 255 165 343 2.21e-16 79.7
MS.gene53254.t1 MTR_2g437700 30.769 156 97 4 92 242 194 343 1.18e-13 71.6
MS.gene53254.t1 MTR_5g098420 30.978 184 103 7 76 255 188 351 4.27e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53254.t1 AT5G03170 51.579 190 81 6 71 258 18 198 2.46e-53 175
MS.gene53254.t1 AT5G60490 57.718 149 57 3 93 239 39 183 2.65e-49 165
MS.gene53254.t1 AT2G20520 41.451 193 103 6 66 255 15 200 9.35e-32 119
MS.gene53254.t1 AT5G44130 40.719 167 92 4 92 255 35 197 3.84e-28 109
MS.gene53254.t1 AT1G03870 34.842 221 123 6 92 310 38 239 1.36e-27 108
MS.gene53254.t1 AT3G46550 31.492 181 113 4 80 255 194 368 1.02e-17 83.6
MS.gene53254.t1 AT3G60900 30.928 194 119 4 79 270 180 360 3.75e-13 70.1
MS.gene53254.t1 AT2G04780 31.333 150 95 4 92 239 45 188 1.91e-11 63.5
MS.gene53254.t1 AT2G04780 31.333 150 95 4 92 239 45 188 1.91e-11 63.5

Find 87 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCAATGGTGGGTCCTTT+TGG 0.235120 4.1:+13201190 None:intergenic
GCCTCTTGATCTTGTTCTTC+CGG 0.272392 4.1:-13200735 MS.gene53254:CDS
TAGCAAACTTGCTATTTATC+AGG 0.275231 4.1:-13200774 MS.gene53254:CDS
GGTGCTGTTGCTGTTGTTGC+TGG 0.314474 4.1:+13201286 None:intergenic
GTTGCTGTTGTTGCTGGTAA+TGG 0.333085 4.1:+13201292 None:intergenic
GAGGAACCCTGCTTTGAGTT+TGG 0.336926 4.1:+13201015 None:intergenic
AGAGTTGGGCTAATGTGGTT+GGG 0.351783 4.1:+13201380 None:intergenic
GCAGTTAATGCTACCGTTAC+TGG 0.358591 4.1:-13200812 MS.gene53254:CDS
TTGGAGAATGCAGAATCTTC+TGG 0.359282 4.1:+13201034 None:intergenic
GTTGGTGGAGTTGTTCTATA+AGG 0.359667 4.1:-13200521 None:intergenic
CCTGCTGATGTTTCTGCTGC+AGG 0.360987 4.1:-13200587 MS.gene53254:CDS
TTTGGCTCCTGCACCGGCTA+AGG 0.362528 4.1:-13200699 MS.gene53254:CDS
CAGTGGCAATCAAGTTAGTA+TGG 0.363282 4.1:-13200843 MS.gene53254:CDS
TCCGGCTAAGGCTCCAGCTT+TGG 0.363612 4.1:-13200717 MS.gene53254:CDS
TCACCGGCTTGTGTTTGAAC+AGG 0.366430 4.1:+13200899 None:intergenic
GAGAGTTGGGCTAATGTGGT+TGG 0.368809 4.1:+13201379 None:intergenic
GGTTTGAATTGGTGAGAGTT+GGG 0.383872 4.1:+13201366 None:intergenic
GGGTTTGAATTGGTGAGAGT+TGG 0.392657 4.1:+13201365 None:intergenic
TGCTATTTATCAGGTTGATA+AGG 0.394299 4.1:-13200765 MS.gene53254:CDS
AATTAGAACGGAGAACCTTT+TGG 0.395304 4.1:+13201147 None:intergenic
GTGAGGGTGATGATATTGTT+GGG 0.400745 4.1:+13201344 None:intergenic
TTTGGAATGGGTTTGGTTGG+TGG 0.406116 4.1:-13200536 MS.gene53254:CDS
GGTGCACCAGTTGGTGTTGT+TGG 0.418778 4.1:+13201232 None:intergenic
TGATCTTGTTCTTCCGGCTA+AGG 0.420269 4.1:-13200729 MS.gene53254:CDS
GGTGAGGGTGATGATATTGT+TGG 0.425491 4.1:+13201343 None:intergenic
GATATTGTTGGGGTTTGAAT+TGG 0.430089 4.1:+13201355 None:intergenic
GCCGGTGCAGGAGCCAAAGC+TGG 0.431263 4.1:+13200704 None:intergenic
TGTTTGAACAGGGTTGGTTA+AGG 0.439720 4.1:+13200910 None:intergenic
CTGCTGCAGGATCTGTGCAT+TGG 0.442652 4.1:-13200574 MS.gene53254:CDS
AAGGGTTTGTTGCCTAAGGC+TGG 0.443081 4.1:-13200680 MS.gene53254:CDS
GTATCATCACCATCAGGTTC+AGG 0.443291 4.1:-13201076 MS.gene53254:CDS
CTTGTTCCAACAACACCAAC+TGG 0.444147 4.1:-13201238 MS.gene53254:CDS
GGTGGTGATGCTGTTGTTGA+TGG 0.445870 4.1:+13201313 None:intergenic
TGATGCTGTTGTTGATGGTA+AGG 0.449757 4.1:+13201318 None:intergenic
AAGAATGTTGATTATGTCAA+TGG 0.455371 4.1:+13201177 None:intergenic
TTGGCTCCTGCACCGGCTAA+GGG 0.463641 4.1:-13200698 MS.gene53254:CDS
AACAAACCCTTAGCCGGTGC+AGG 0.463951 4.1:+13200692 None:intergenic
GCATTCTCCAAACTCAAAGC+AGG 0.466381 4.1:-13201022 MS.gene53254:CDS
GGCTAAGGGTTTGTTGCCTA+AGG 0.468336 4.1:-13200684 MS.gene53254:CDS
AATTCTTCGGTTGTAGATGA+TGG 0.470488 4.1:-13200650 MS.gene53254:CDS
GAGTTGGGCTAATGTGGTTG+GGG 0.472510 4.1:+13201381 None:intergenic
GTAGATGATGGTAGCGGTGC+TGG 0.479324 4.1:-13200638 MS.gene53254:CDS
TCCAGCTTTGGCTCCTGCAC+CGG 0.487588 4.1:-13200705 MS.gene53254:CDS
GTCTATCAGTGAGGGAGTTG+AGG 0.498053 4.1:+13200996 None:intergenic
TGAGGGTGATGATATTGTTG+GGG 0.500322 4.1:+13201345 None:intergenic
AGCGGAACTGTGCTTAATCC+TGG 0.504963 4.1:+13201259 None:intergenic
TGGTGAGAGTTGGGCTAATG+TGG 0.506138 4.1:+13201375 None:intergenic
GGCTTGTGTTTGAACAGGGT+TGG 0.506531 4.1:+13200904 None:intergenic
GTTGATGGTAAGGGTGGTGA+GGG 0.507310 4.1:+13201328 None:intergenic
CACCGGCTTGTGTTTGAACA+GGG 0.508119 4.1:+13200900 None:intergenic
ATACTAGCTGCGAGTTGAGT+TGG 0.509205 4.1:+13201095 None:intergenic
GCCAAAGCTGGAGCCTTAGC+CGG 0.517768 4.1:+13200716 None:intergenic
AACGGTAGCATTAACTGCTC+CGG 0.527378 4.1:+13200817 None:intergenic
TGTTGATGGTAAGGGTGGTG+AGG 0.528646 4.1:+13201327 None:intergenic
CAGCTAGTATCATCACCATC+AGG 0.530331 4.1:-13201082 MS.gene53254:CDS
CCCACCAAAGCAATCCCAAA+AGG 0.532318 4.1:-13201205 MS.gene53254:CDS
AAATAGAAACAAACGAAGCA+AGG 0.540997 4.1:+13200945 None:intergenic
AGCGGTGCTGGCAGTGATGA+TGG 0.560075 4.1:-13200626 MS.gene53254:CDS
TTAGGCAACAAACCCTTAGC+CGG 0.560846 4.1:+13200686 None:intergenic
ATAAATAGCAAGTTTGCTAT+CGG 0.562446 4.1:+13200778 None:intergenic
GTTGGTGTTGTTGGAACAAG+CGG 0.574823 4.1:+13201241 None:intergenic
AACCCTGTTCAAACACAAGC+CGG 0.576254 4.1:-13200902 MS.gene53254:CDS
AATTGCTAGAAGAAAGAATG+AGG 0.576326 4.1:+13201414 None:intergenic
GCTGTTGTTGATGGTAAGGG+TGG 0.581658 4.1:+13201322 None:intergenic
GCTGTTGTTGCTGGTAATGG+TGG 0.582499 4.1:+13201295 None:intergenic
CACAAGCCGGTGATGATGCT+AGG 0.584174 4.1:-13200889 MS.gene53254:CDS
AATCAAGTTAGTATGGCTAC+CGG 0.593663 4.1:-13200836 MS.gene53254:CDS
TCGGTTGTAGATGATGGTAG+CGG 0.599850 4.1:-13200644 MS.gene53254:CDS
ACAGCAACAGCACCATCACC+AGG 0.599988 4.1:-13201277 MS.gene53254:CDS
GATGCTGTTGTTGATGGTAA+GGG 0.606751 4.1:+13201319 None:intergenic
CCTGCAGCAGAAACATCAGC+AGG 0.607919 4.1:+13200587 None:intergenic
TCATCACCATCAGGTTCAGG+AGG 0.610251 4.1:-13201073 MS.gene53254:CDS
CATTCTCCAAACTCAAAGCA+GGG 0.610410 4.1:-13201021 MS.gene53254:CDS
TTTGAGAAGACGAATTAGAA+CGG 0.612652 4.1:+13201135 None:intergenic
GCCGGAAGAACAAGATCAAG+AGG 0.613702 4.1:+13200734 None:intergenic
AATGTTGATTATGTCAATGG+TGG 0.616748 4.1:+13201180 None:intergenic
AATAGAAACAAACGAAGCAA+GGG 0.620497 4.1:+13200946 None:intergenic
TTGAAGCCTAGCATCATCAC+CGG 0.621788 4.1:+13200883 None:intergenic
TGCTAGAAGAAAGAATGAGG+TGG 0.621900 4.1:+13201417 None:intergenic
CAGTGATGATGGTGATGGAA+AGG 0.627687 4.1:-13200615 MS.gene53254:CDS
ACTGTGCTTAATCCTGGTGA+TGG 0.634396 4.1:+13201265 None:intergenic
GGTATAAACAGTACCAGTAA+CGG 0.640082 4.1:+13200799 None:intergenic
GCTGGCAGTGATGATGGTGA+TGG 0.645590 4.1:-13200620 MS.gene53254:CDS
GTTAACCCTCCTGAACCTGA+TGG 0.664853 4.1:+13201067 None:intergenic
CTGAACGTCACTACTTACAG+TGG 0.677815 4.1:-13200860 MS.gene53254:CDS
ATGTTGATTATGTCAATGGT+GGG 0.681774 4.1:+13201181 None:intergenic
CATCACCATCAGGTTCAGGA+GGG 0.700637 4.1:-13201072 MS.gene53254:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATGTTGATTATGTCAA+TGG + chr4.1:13200797-13200816 None:intergenic 25.0%
!!! AATGTTGTTGTTTTTGGAAT+GGG - chr4.1:13201423-13201442 MS.gene53254:CDS 25.0%
!!! ATAAATAGCAAGTTTGCTAT+CGG + chr4.1:13201196-13201215 None:intergenic 25.0%
AAATAGAAACAAACGAAGCA+AGG + chr4.1:13201029-13201048 None:intergenic 30.0%
AATAGAAACAAACGAAGCAA+GGG + chr4.1:13201028-13201047 None:intergenic 30.0%
AATTGCTAGAAGAAAGAATG+AGG + chr4.1:13200560-13200579 None:intergenic 30.0%
ACATTCTTCAAAAAGCCAAA+AGG - chr4.1:13200809-13200828 MS.gene53254:CDS 30.0%
TTTGAGAAGACGAATTAGAA+CGG + chr4.1:13200839-13200858 None:intergenic 30.0%
! AACTTTTTGTCTATCAGTGA+GGG + chr4.1:13200986-13201005 None:intergenic 30.0%
! AATGTTGATTATGTCAATGG+TGG + chr4.1:13200794-13200813 None:intergenic 30.0%
! ATGTTGATTATGTCAATGGT+GGG + chr4.1:13200793-13200812 None:intergenic 30.0%
!! TAGCAAACTTGCTATTTATC+AGG - chr4.1:13201197-13201216 MS.gene53254:CDS 30.0%
!! TGCTATTTATCAGGTTGATA+AGG - chr4.1:13201206-13201225 MS.gene53254:CDS 30.0%
!!! GAATGTTGTTGTTTTTGGAA+TGG - chr4.1:13201422-13201441 MS.gene53254:CDS 30.0%
AATCAAGTTAGTATGGCTAC+CGG - chr4.1:13201135-13201154 MS.gene53254:CDS 35.0%
AATTCTTCGGTTGTAGATGA+TGG - chr4.1:13201321-13201340 MS.gene53254:CDS 35.0%
AGAATCTTCTGGTGCAAAAA+TGG + chr4.1:13200929-13200948 None:intergenic 35.0%
GATATTGTTGGGGTTTGAAT+TGG + chr4.1:13200619-13200638 None:intergenic 35.0%
GGCTGGAAAAACAAATTCTT+CGG - chr4.1:13201308-13201327 MS.gene53254:CDS 35.0%
GGTATAAACAGTACCAGTAA+CGG + chr4.1:13201175-13201194 None:intergenic 35.0%
! AATTAGAACGGAGAACCTTT+TGG + chr4.1:13200827-13200846 None:intergenic 35.0%
! CAACTTTTTGTCTATCAGTG+AGG + chr4.1:13200987-13201006 None:intergenic 35.0%
!! GAATTTGTTTTTCCAGCCTT+AGG + chr4.1:13201306-13201325 None:intergenic 35.0%
!!! TGGCTGAATGTTGTTGTTTT+TGG - chr4.1:13201417-13201436 MS.gene53254:CDS 35.0%
!!! TGTTGTTTTTGGAATGGGTT+TGG - chr4.1:13201428-13201447 MS.gene53254:CDS 35.0%
CAGTGGCAATCAAGTTAGTA+TGG - chr4.1:13201128-13201147 MS.gene53254:CDS 40.0%
CATTCTCCAAACTCAAAGCA+GGG - chr4.1:13200950-13200969 MS.gene53254:CDS 40.0%
GGTTTGAATTGGTGAGAGTT+GGG + chr4.1:13200608-13200627 None:intergenic 40.0%
GTGAGGGTGATGATATTGTT+GGG + chr4.1:13200630-13200649 None:intergenic 40.0%
TGAGGGTGATGATATTGTTG+GGG + chr4.1:13200629-13200648 None:intergenic 40.0%
TGCTAGAAGAAAGAATGAGG+TGG + chr4.1:13200557-13200576 None:intergenic 40.0%
TTGGAGAATGCAGAATCTTC+TGG + chr4.1:13200940-13200959 None:intergenic 40.0%
!! GATGCTGTTGTTGATGGTAA+GGG + chr4.1:13200655-13200674 None:intergenic 40.0%
!! TGATGCTGTTGTTGATGGTA+AGG + chr4.1:13200656-13200675 None:intergenic 40.0%
!! TGTTTGAACAGGGTTGGTTA+AGG + chr4.1:13201064-13201083 None:intergenic 40.0%
!!! GTTTTTGGAATGGGTTTGGT+TGG - chr4.1:13201432-13201451 MS.gene53254:CDS 40.0%
AACCCTGTTCAAACACAAGC+CGG - chr4.1:13201069-13201088 MS.gene53254:CDS 45.0%
AACGGTAGCATTAACTGCTC+CGG + chr4.1:13201157-13201176 None:intergenic 45.0%
ACTGTGCTTAATCCTGGTGA+TGG + chr4.1:13200709-13200728 None:intergenic 45.0%
AGAGTTGGGCTAATGTGGTT+GGG + chr4.1:13200594-13200613 None:intergenic 45.0%
CAGCTAGTATCATCACCATC+AGG - chr4.1:13200889-13200908 MS.gene53254:CDS 45.0%
CTGAACGTCACTACTTACAG+TGG - chr4.1:13201111-13201130 MS.gene53254:CDS 45.0%
CTTGTTCCAACAACACCAAC+TGG - chr4.1:13200733-13200752 MS.gene53254:CDS 45.0%
GCAGTTAATGCTACCGTTAC+TGG - chr4.1:13201159-13201178 MS.gene53254:CDS 45.0%
GCATTCTCCAAACTCAAAGC+AGG - chr4.1:13200949-13200968 MS.gene53254:CDS 45.0%
GCCTCTTGATCTTGTTCTTC+CGG - chr4.1:13201236-13201255 MS.gene53254:CDS 45.0%
GGGTTTGAATTGGTGAGAGT+TGG + chr4.1:13200609-13200628 None:intergenic 45.0%
GGTGAGGGTGATGATATTGT+TGG + chr4.1:13200631-13200650 None:intergenic 45.0%
GTATCATCACCATCAGGTTC+AGG - chr4.1:13200895-13200914 MS.gene53254:CDS 45.0%
TGATCTTGTTCTTCCGGCTA+AGG - chr4.1:13201242-13201261 MS.gene53254:CDS 45.0%
TTAGGCAACAAACCCTTAGC+CGG + chr4.1:13201288-13201307 None:intergenic 45.0%
TTGAAGCCTAGCATCATCAC+CGG + chr4.1:13201091-13201110 None:intergenic 45.0%
! ATACTAGCTGCGAGTTGAGT+TGG + chr4.1:13200879-13200898 None:intergenic 45.0%
! GTTGCTGTTGTTGCTGGTAA+TGG + chr4.1:13200682-13200701 None:intergenic 45.0%
! TCGGTTGTAGATGATGGTAG+CGG - chr4.1:13201327-13201346 MS.gene53254:CDS 45.0%
! TTTGGAATGGGTTTGGTTGG+TGG - chr4.1:13201435-13201454 MS.gene53254:CDS 45.0%
!! CAGTGATGATGGTGATGGAA+AGG - chr4.1:13201356-13201375 MS.gene53254:CDS 45.0%
!! GTTGGTGTTGTTGGAACAAG+CGG + chr4.1:13200733-13200752 None:intergenic 45.0%
!!! ATGTCAATGGTGGGTCCTTT+TGG + chr4.1:13200784-13200803 None:intergenic 45.0%
!!! CCTTTTGGGATTGCTTTGGT+GGG + chr4.1:13200769-13200788 None:intergenic 45.0%
!!! CTTTTGGGATTGCTTTGGTG+GGG + chr4.1:13200768-13200787 None:intergenic 45.0%
!!! TCCTTTTGGGATTGCTTTGG+TGG + chr4.1:13200770-13200789 None:intergenic 45.0%
!!! TGTCAATGGTGGGTCCTTTT+GGG + chr4.1:13200783-13200802 None:intergenic 45.0%
!!! TTTTGGGATTGCTTTGGTGG+GGG + chr4.1:13200767-13200786 None:intergenic 45.0%
AGCGGAACTGTGCTTAATCC+TGG + chr4.1:13200715-13200734 None:intergenic 50.0%
CATCACCATCAGGTTCAGGA+GGG - chr4.1:13200899-13200918 MS.gene53254:CDS 50.0%
CCCACCAAAGCAATCCCAAA+AGG - chr4.1:13200766-13200785 MS.gene53254:CDS 50.0%
GAGAGTTGGGCTAATGTGGT+TGG + chr4.1:13200595-13200614 None:intergenic 50.0%
GAGTTGGGCTAATGTGGTTG+GGG + chr4.1:13200593-13200612 None:intergenic 50.0%
GCCGGAAGAACAAGATCAAG+AGG + chr4.1:13201240-13201259 None:intergenic 50.0%
GGCTTGTGTTTGAACAGGGT+TGG + chr4.1:13201070-13201089 None:intergenic 50.0%
GTCTATCAGTGAGGGAGTTG+AGG + chr4.1:13200978-13200997 None:intergenic 50.0%
GTTAACCCTCCTGAACCTGA+TGG + chr4.1:13200907-13200926 None:intergenic 50.0%
TCATCACCATCAGGTTCAGG+AGG - chr4.1:13200898-13200917 MS.gene53254:CDS 50.0%
TGGTGAGAGTTGGGCTAATG+TGG + chr4.1:13200599-13200618 None:intergenic 50.0%
! GAGGAACCCTGCTTTGAGTT+TGG + chr4.1:13200959-13200978 None:intergenic 50.0%
! GGTGGTGATGCTGTTGTTGA+TGG + chr4.1:13200661-13200680 None:intergenic 50.0%
!! AAGGGTTTGTTGCCTAAGGC+TGG - chr4.1:13201291-13201310 MS.gene53254:CDS 50.0%
!! CACCGGCTTGTGTTTGAACA+GGG + chr4.1:13201074-13201093 None:intergenic 50.0%
!! GCTGTTGTTGATGGTAAGGG+TGG + chr4.1:13200652-13200671 None:intergenic 50.0%
!! GCTGTTGTTGCTGGTAATGG+TGG + chr4.1:13200679-13200698 None:intergenic 50.0%
!! GGCTAAGGGTTTGTTGCCTA+AGG - chr4.1:13201287-13201306 MS.gene53254:CDS 50.0%
!! GTTGATGGTAAGGGTGGTGA+GGG + chr4.1:13200646-13200665 None:intergenic 50.0%
!! TCACCGGCTTGTGTTTGAAC+AGG + chr4.1:13201075-13201094 None:intergenic 50.0%
!! TGTTGATGGTAAGGGTGGTG+AGG + chr4.1:13200647-13200666 None:intergenic 50.0%
!!! GGGTCCTTTTGGGATTGCTT+TGG + chr4.1:13200773-13200792 None:intergenic 50.0%
AACAAACCCTTAGCCGGTGC+AGG + chr4.1:13201282-13201301 None:intergenic 55.0%
ACAGCAACAGCACCATCACC+AGG - chr4.1:13200694-13200713 MS.gene53254:CDS 55.0%
CCTGCAGCAGAAACATCAGC+AGG + chr4.1:13201387-13201406 None:intergenic 55.0%
CTGCTGCAGGATCTGTGCAT+TGG - chr4.1:13201397-13201416 MS.gene53254:CDS 55.0%
! CACAAGCCGGTGATGATGCT+AGG - chr4.1:13201082-13201101 MS.gene53254:CDS 55.0%
! CCTGCTGATGTTTCTGCTGC+AGG - chr4.1:13201384-13201403 MS.gene53254:CDS 55.0%
!! GCTGGCAGTGATGATGGTGA+TGG - chr4.1:13201351-13201370 MS.gene53254:CDS 55.0%
!! GGTGCACCAGTTGGTGTTGT+TGG + chr4.1:13200742-13200761 None:intergenic 55.0%
!! GGTGCTGTTGCTGTTGTTGC+TGG + chr4.1:13200688-13200707 None:intergenic 55.0%
!! GTAGATGATGGTAGCGGTGC+TGG - chr4.1:13201333-13201352 MS.gene53254:CDS 55.0%
GCCAAAGCTGGAGCCTTAGC+CGG + chr4.1:13201258-13201277 None:intergenic 60.0%
GGGATTGCTTTGGTGGGGGA+TGG + chr4.1:13200763-13200782 None:intergenic 60.0%
TCCGGCTAAGGCTCCAGCTT+TGG - chr4.1:13201254-13201273 MS.gene53254:CDS 60.0%
! TCCAGCTTTGGCTCCTGCAC+CGG - chr4.1:13201266-13201285 MS.gene53254:CDS 60.0%
!! AGCGGTGCTGGCAGTGATGA+TGG - chr4.1:13201345-13201364 MS.gene53254:CDS 60.0%
!! TTGGCTCCTGCACCGGCTAA+GGG - chr4.1:13201273-13201292 MS.gene53254:CDS 60.0%
!! TTTGGCTCCTGCACCGGCTA+AGG - chr4.1:13201272-13201291 MS.gene53254:CDS 60.0%
GTGGGGGATGGTGCACCAGT+TGG + chr4.1:13200751-13200770 None:intergenic 65.0%
GCCGGTGCAGGAGCCAAAGC+TGG + chr4.1:13201270-13201289 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13200523 13201470 13200523 ID=MS.gene53254
chr4.1 mRNA 13200523 13201470 13200523 ID=MS.gene53254.t1;Parent=MS.gene53254
chr4.1 exon 13200523 13201470 13200523 ID=MS.gene53254.t1.exon1;Parent=MS.gene53254.t1
chr4.1 CDS 13200523 13201470 13200523 ID=cds.MS.gene53254.t1;Parent=MS.gene53254.t1
Gene Sequence

>MS.gene53254

ATGAAAAATATGATGCAACAGTCTCTCATTTCCACCTCATTCTTTCTTCTAGCAATTACTTTCTACACCCCAACCACATTAGCCCAACTCTCACCAATTCAAACCCCAACAATATCATCACCCTCACCACCCTTACCATCAACAACAGCATCACCACCATTACCAGCAACAACAGCAACAGCACCATCACCAGGATTAAGCACAGTTCCGCTTGTTCCAACAACACCAACTGGTGCACCATCCCCCACCAAAGCAATCCCAAAAGGACCCACCATTGACATAATCAACATTCTTCAAAAAGCCAAAAGGTTCTCCGTTCTAATTCGTCTTCTCAAAACAACACAATTAATAAACCAACTCAACTCGCAGCTAGTATCATCACCATCAGGTTCAGGAGGGTTAACCATTTTTGCACCAGAAGATTCTGCATTCTCCAAACTCAAAGCAGGGTTCCTCAACTCCCTCACTGATAGACAAAAAGTTGAACTCTTACAGTTTCACTCCCTTGCTTCGTTTGTTTCTATTTCAAACTTTGATACCTTAACCAACCCTGTTCAAACACAAGCCGGTGATGATGCTAGGCTTCAACTGAACGTCACTACTTACAGTGGCAATCAAGTTAGTATGGCTACCGGAGCAGTTAATGCTACCGTTACTGGTACTGTTTATACCGATAGCAAACTTGCTATTTATCAGGTTGATAAGGTACTTATGCCTCTTGATCTTGTTCTTCCGGCTAAGGCTCCAGCTTTGGCTCCTGCACCGGCTAAGGGTTTGTTGCCTAAGGCTGGAAAAACAAATTCTTCGGTTGTAGATGATGGTAGCGGTGCTGGCAGTGATGATGGTGATGGAAAGGCTTTACCTGCTGATGTTTCTGCTGCAGGATCTGTGCATTGGCTGAATGTTGTTGTTTTTGGAATGGGTTTGGTTGGTGGAGTTGTTCTATAA
Protein sequence

>MS.gene53254.t1

MKNMMQQSLISTSFFLLAITFYTPTTLAQLSPIQTPTISSPSPPLPSTTASPPLPATTATAPSPGLSTVPLVPTTPTGAPSPTKAIPKGPTIDIINILQKAKRFSVLIRLLKTTQLINQLNSQLVSSPSGSGGLTIFAPEDSAFSKLKAGFLNSLTDRQKVELLQFHSLASFVSISNFDTLTNPVQTQAGDDARLQLNVTTYSGNQVSMATGAVNATVTGTVYTDSKLAIYQVDKVLMPLDLVLPAKAPALAPAPAKGLLPKAGKTNSSVVDDGSGAGSDDGDGKALPADVSAAGSVHWLNVVVFGMGLVGGVVL