AlfalfaGEDB | MS.gene53259

M. sativa cultivar XinJiangDaYe / MS.gene53259

KEGG: -
GO: -
PFAM: PF02469.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53259.t1	RHN42678.1	94.5	292	16	0	1	292	1	292	2.00E-98	369

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53259.t1	Q8LEE9	51.8	164	77	2	65	227	27	189	6.5e-37	156.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53259.t1	G7L792	94.5	292	16	0	1	292	1	292	1.5e-98	369.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056470	MS.gene53259	0.892385	1.67E-74	-1.69E-46
MS.gene058848	MS.gene53259	0.925727	1.24E-90	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53259.t1	MTR_8g087450	96.918	292	9	0	1	292	1	292	0.0	566
MS.gene53259.t1	MTR_2g017960	71.379	290	66	6	1	289	1	274	3.09e-116	335
MS.gene53259.t1	MTR_2g017970	77.143	210	46	2	76	285	67	274	1.61e-109	318
MS.gene53259.t1	MTR_4g059840	55.187	241	93	6	58	288	53	288	3.51e-80	244
MS.gene53259.t1	MTR_4g053380	49.836	305	121	8	2	291	7	294	2.22e-78	240
MS.gene53259.t1	MTR_4g059790	60.199	201	70	3	58	248	53	253	5.49e-78	239
MS.gene53259.t1	MTR_2g093980	48.814	295	130	9	1	288	1	281	9.75e-76	233
MS.gene53259.t1	MTR_4g059780	49.815	271	115	5	21	284	45	301	8.99e-69	216
MS.gene53259.t1	MTR_2g093970	45.890	292	125	7	10	285	13	287	7.21e-68	213
MS.gene53259.t1	MTR_2g017950	43.345	293	139	8	6	285	12	290	2.67e-64	204
MS.gene53259.t1	MTR_8g087470	45.113	266	127	6	41	288	51	315	1.05e-62	201
MS.gene53259.t1	MTR_8g087460	46.212	264	121	7	45	288	53	315	1.05e-61	198
MS.gene53259.t1	MTR_4g059720	45.415	229	84	4	1	228	4	192	1.04e-56	183
MS.gene53259.t1	MTR_2g093990	46.117	206	101	3	77	279	41	239	7.22e-53	173
MS.gene53259.t1	MTR_7g101080	44.578	166	90	2	58	222	21	185	2.35e-41	144
MS.gene53259.t1	MTR_2g090765	35.565	239	132	4	50	286	29	247	1.41e-27	107
MS.gene53259.t1	MTR_5g098060	30.588	170	113	3	77	243	48	215	1.06e-15	75.5
MS.gene53259.t1	MTR_7g075453	31.746	189	113	4	68	250	175	353	3.68e-14	72.4
MS.gene53259.t1	MTR_2g437700	31.544	149	95	4	77	221	195	340	1.88e-12	67.4
MS.gene53259.t1	MTR_5g098420	30.270	185	114	5	55	235	177	350	7.20e-12	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53259.t1	AT5G60490	54.795	146	64	2	77	221	39	183	7.29e-47	158
MS.gene53259.t1	AT5G03170	46.023	176	93	2	70	244	29	203	7.20e-45	152
MS.gene53259.t1	AT5G44130	40.437	183	103	3	77	257	36	214	1.10e-33	123
MS.gene53259.t1	AT2G20520	41.622	185	95	5	77	249	38	221	1.34e-29	112
MS.gene53259.t1	AT1G03870	38.830	188	109	4	77	263	39	221	2.85e-29	112
MS.gene53259.t1	AT2G04780	31.544	149	96	3	75	221	44	188	2.65e-13	68.9
MS.gene53259.t1	AT2G04780	31.544	149	96	3	75	221	44	188	2.65e-13	68.9
MS.gene53259.t1	AT3G46550	28.495	186	120	8	63	243	194	371	1.06e-12	68.2
MS.gene53259.t1	AT3G60900	28.488	172	117	3	70	240	183	349	3.26e-12	67.0

Find 49 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGCTGGAGCTTTAGCTTT+AGG	0.270473	4.1:+13267505	None:intergenic
TTCGAACTTGATACATATTC+AGG	0.279696	4.1:+13267730	None:intergenic
GAATTCTCTTTCTCAGCTTT+AGG	0.296501	4.1:+13267466	None:intergenic
TGCTATATATCATGTGAATA+AGG	0.305049	4.1:-13267554	MS.gene53259:CDS
CAGATTAATGTGACTGCATA+TGG	0.308276	4.1:-13267649	MS.gene53259:CDS
GGTGCTGGAGCAGGTGTTGA+AGG	0.331645	4.1:+13268045	None:intergenic
ATGCATGTTATTCAAGCTAA+TGG	0.337878	4.1:+13267393	None:intergenic
GAATAAGGTTCTTATTCCTT+TGG	0.338628	4.1:-13267539	MS.gene53259:CDS
TCTTGGTTTCCTGACGAATC+AGG	0.343060	4.1:+13267976	None:intergenic
AGCAGGTTACTTCAACTCCT+TGG	0.397163	4.1:-13267794	MS.gene53259:CDS
TGTGGTAATGCTGGGACTAA+TGG	0.407871	4.1:+13268015	None:intergenic
GATTAGTGTGTTGAATGATT+TGG	0.407894	4.1:+13267930	None:intergenic
TTAGAATCTTGATTATGTCT+TGG	0.417666	4.1:+13267959	None:intergenic
GCCTTAGCAAGAGCTGGTGC+TGG	0.431209	4.1:+13267490	None:intergenic
TCTGAAAAGGCACCATCATC+TGG	0.442423	4.1:+13267823	None:intergenic
AGAAGATAGAGGCAAGTAGT+AGG	0.446057	4.1:+13268157	None:intergenic
ATATCAGTGTTGGTTTCCCT+TGG	0.450067	4.1:-13267367	MS.gene53259:CDS
GCAGGTTACTTCAACTCCTT+GGG	0.455965	4.1:-13267793	MS.gene53259:CDS
AACAGTGTGAATATTTCAAC+TGG	0.459076	4.1:-13267625	MS.gene53259:CDS
GTAGGTGATTGTGGTAATGC+TGG	0.464029	4.1:+13268006	None:intergenic
GACGAATCAGGATTTGTTGT+AGG	0.471829	4.1:+13267988	None:intergenic
GTGCTGGAGCAGGTGTTGAA+GGG	0.481443	4.1:+13268046	None:intergenic
CATTAATCTGAAATCCTTGT+GGG	0.483229	4.1:+13267662	None:intergenic
ACCTGCTTTGAGTTCTGAAA+AGG	0.484192	4.1:+13267810	None:intergenic
TTAGGTGCCTTAGCAAGAGC+TGG	0.489973	4.1:+13267484	None:intergenic
GGATTTGTTGTAGGTGATTG+TGG	0.513333	4.1:+13267997	None:intergenic
ACAATTCTTGCACCAGATGA+TGG	0.514841	4.1:-13267835	MS.gene53259:CDS
GGAGCAGGTGTTGAAGGGGT+AGG	0.521942	4.1:+13268051	None:intergenic
ACAACAAATCCTGATTCGTC+AGG	0.522883	4.1:-13267985	MS.gene53259:CDS
AGTGGTGATTGAGAAGATAG+AGG	0.531658	4.1:+13268146	None:intergenic
TTTGTGTTGTCTTGAGCAAA+CGG	0.534377	4.1:+13267908	None:intergenic
TCCAGCACCAGCTCTTGCTA+AGG	0.542557	4.1:-13267491	MS.gene53259:CDS
AGCGTAAACGATTCCAGTGA+TGG	0.545525	4.1:+13267588	None:intergenic
TTACAAGCTGTGCATTGACT+TGG	0.551173	4.1:+13267878	None:intergenic
CATGCATGGAATATCAGTGT+TGG	0.573529	4.1:-13267377	MS.gene53259:CDS
TGCTGGAGCAGGTGTTGAAG+GGG	0.574142	4.1:+13268047	None:intergenic
ACATTAATCTGAAATCCTTG+TGG	0.574915	4.1:+13267661	None:intergenic
CTGCAAGCAGAGAAATTCCA+AGG	0.575775	4.1:+13267350	None:intergenic
AGCTGGTGATTGAGCTAAAG+TGG	0.578327	4.1:+13268128	None:intergenic
ATTAGCTTGAATAACATGCA+TGG	0.579278	4.1:-13267391	MS.gene53259:CDS
TAGGTGATTGTGGTAATGCT+GGG	0.582717	4.1:+13268007	None:intergenic
GTTGTTGATACAACCATCAC+TGG	0.593007	4.1:-13267601	MS.gene53259:CDS
TTAGGCTTAACAAAATCCAA+AGG	0.601169	4.1:+13267523	None:intergenic
CTAGCTAGTGATAGCCCACA+AGG	0.607212	4.1:-13267676	MS.gene53259:CDS
TGATCAGACTCAGGCACACA+AGG	0.622744	4.1:-13267428	MS.gene53259:CDS
AGATGATGATGATCAGACTC+AGG	0.626792	4.1:-13267437	MS.gene53259:CDS
CACTAGCTAGTGTCAACACA+GGG	0.648522	4.1:+13267689	None:intergenic
TGCAAGCAGAGAAATTCCAA+GGG	0.658685	4.1:+13267351	None:intergenic
TCACTAGCTAGTGTCAACAC+AGG	0.685638	4.1:+13267688	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGCTATATATCATGTGAATA+AGG	-	chr4.1:13267956-13267975	MS.gene53259:CDS	25.0%
!	TTAGAATCTTGATTATGTCT+TGG	+	chr4.1:13267554-13267573	None:intergenic	25.0%
	AACAGTGTGAATATTTCAAC+TGG	-	chr4.1:13267885-13267904	MS.gene53259:CDS	30.0%
	ATGCATGTTATTCAAGCTAA+TGG	+	chr4.1:13268120-13268139	None:intergenic	30.0%
	CAGCTTGTAACTACAAAAAA+TGG	-	chr4.1:13267645-13267664	MS.gene53259:CDS	30.0%
	CTTGTAACTACAAAAAATGG+AGG	-	chr4.1:13267648-13267667	MS.gene53259:CDS	30.0%
	GAATAAGGTTCTTATTCCTT+TGG	-	chr4.1:13267971-13267990	MS.gene53259:CDS	30.0%
	GATTAGTGTGTTGAATGATT+TGG	+	chr4.1:13267583-13267602	None:intergenic	30.0%
	TTAGGCTTAACAAAATCCAA+AGG	+	chr4.1:13267990-13268009	None:intergenic	30.0%
	TTCGAACTTGATACATATTC+AGG	+	chr4.1:13267783-13267802	None:intergenic	30.0%
!	ACATTAATCTGAAATCCTTG+TGG	+	chr4.1:13267852-13267871	None:intergenic	30.0%
!	ATTAGCTTGAATAACATGCA+TGG	-	chr4.1:13268119-13268138	MS.gene53259:CDS	30.0%
!	CATTAATCTGAAATCCTTGT+GGG	+	chr4.1:13267851-13267870	None:intergenic	30.0%
	CAGATTAATGTGACTGCATA+TGG	-	chr4.1:13267861-13267880	MS.gene53259:CDS	35.0%
	GAATTCTCTTTCTCAGCTTT+AGG	+	chr4.1:13268047-13268066	None:intergenic	35.0%
	TTTGTGTTGTCTTGAGCAAA+CGG	+	chr4.1:13267605-13267624	None:intergenic	35.0%
!!	TTTTTGTTGTCTTTCTCCCA+AGG	+	chr4.1:13267736-13267755	None:intergenic	35.0%
	ACAATTCTTGCACCAGATGA+TGG	-	chr4.1:13267675-13267694	MS.gene53259:CDS	40.0%
	AGAAGATAGAGGCAAGTAGT+AGG	+	chr4.1:13267356-13267375	None:intergenic	40.0%
	AGTGGTGATTGAGAAGATAG+AGG	+	chr4.1:13267367-13267386	None:intergenic	40.0%
	CATGCATGGAATATCAGTGT+TGG	-	chr4.1:13268133-13268152	MS.gene53259:CDS	40.0%
	GTTGTTGATACAACCATCAC+TGG	-	chr4.1:13267909-13267928	MS.gene53259:CDS	40.0%
	TGCAAGCAGAGAAATTCCAA+GGG	+	chr4.1:13268162-13268181	None:intergenic	40.0%
!	ACAACAAATCCTGATTCGTC+AGG	-	chr4.1:13267525-13267544	MS.gene53259:CDS	40.0%
!	AGATGATGATGATCAGACTC+AGG	-	chr4.1:13268073-13268092	MS.gene53259:CDS	40.0%
!	TAGGTGATTGTGGTAATGCT+GGG	+	chr4.1:13267506-13267525	None:intergenic	40.0%
!!	ACCTGCTTTGAGTTCTGAAA+AGG	+	chr4.1:13267703-13267722	None:intergenic	40.0%
!!	ATATCAGTGTTGGTTTCCCT+TGG	-	chr4.1:13268143-13268162	MS.gene53259:CDS	40.0%
!!	GACGAATCAGGATTTGTTGT+AGG	+	chr4.1:13267525-13267544	None:intergenic	40.0%
!!	GGATTTGTTGTAGGTGATTG+TGG	+	chr4.1:13267516-13267535	None:intergenic	40.0%
!!	TTACAAGCTGTGCATTGACT+TGG	+	chr4.1:13267635-13267654	None:intergenic	40.0%
!!	TTGAAGGGGTAGGTTTTGTA+GGG	+	chr4.1:13267452-13267471	None:intergenic	40.0%
!!!	TTTGTAGGTGCTGGTTTTTC+TGG	+	chr4.1:13267423-13267442	None:intergenic	40.0%
	AGCAGGTTACTTCAACTCCT+TGG	-	chr4.1:13267716-13267735	MS.gene53259:CDS	45.0%
	AGCGTAAACGATTCCAGTGA+TGG	+	chr4.1:13267925-13267944	None:intergenic	45.0%
	AGCTGGTGATTGAGCTAAAG+TGG	+	chr4.1:13267385-13267404	None:intergenic	45.0%
	CACTAGCTAGTGTCAACACA+GGG	+	chr4.1:13267824-13267843	None:intergenic	45.0%
	CTGCAAGCAGAGAAATTCCA+AGG	+	chr4.1:13268163-13268182	None:intergenic	45.0%
	GCAGGTTACTTCAACTCCTT+GGG	-	chr4.1:13267717-13267736	MS.gene53259:CDS	45.0%
	TCACTAGCTAGTGTCAACAC+AGG	+	chr4.1:13267825-13267844	None:intergenic	45.0%
	TCTTGGTTTCCTGACGAATC+AGG	+	chr4.1:13267537-13267556	None:intergenic	45.0%
!	GCCTTTTCAGAACTCAAAGC+AGG	-	chr4.1:13267699-13267718	MS.gene53259:CDS	45.0%
!	GTAGGTGATTGTGGTAATGC+TGG	+	chr4.1:13267507-13267526	None:intergenic	45.0%
!	TGTGGTAATGCTGGGACTAA+TGG	+	chr4.1:13267498-13267517	None:intergenic	45.0%
!!	ACTAATGGTTTTGCTGGTGC+TGG	+	chr4.1:13267483-13267502	None:intergenic	45.0%
!!	GTTGAAGGGGTAGGTTTTGT+AGG	+	chr4.1:13267453-13267472	None:intergenic	45.0%
!!	TCTGAAAAGGCACCATCATC+TGG	+	chr4.1:13267690-13267709	None:intergenic	45.0%
!!	TGAAGGGGTAGGTTTTGTAG+GGG	+	chr4.1:13267451-13267470	None:intergenic	45.0%
!!!	GCTGGTTTTTCTGGTGCTTT+TGG	+	chr4.1:13267414-13267433	None:intergenic	45.0%
!!!	TTTGTAGGGGCTGGTTTTGT+AGG	+	chr4.1:13267438-13267457	None:intergenic	45.0%
	CTAGCTAGTGATAGCCCACA+AGG	-	chr4.1:13267834-13267853	MS.gene53259:CDS	50.0%
	TGATCAGACTCAGGCACACA+AGG	-	chr4.1:13268082-13268101	MS.gene53259:CDS	50.0%
	TTAGGTGCCTTAGCAAGAGC+TGG	+	chr4.1:13268029-13268048	None:intergenic	50.0%
!!	GCTGGGACTAATGGTTTTGC+TGG	+	chr4.1:13267489-13267508	None:intergenic	50.0%
!!	GGTGCTGGAGCTTTAGCTTT+AGG	+	chr4.1:13268008-13268027	None:intergenic	50.0%
	TCCAGCACCAGCTCTTGCTA+AGG	-	chr4.1:13268019-13268038	MS.gene53259:CDS	55.0%
!!	GTGCTGGAGCAGGTGTTGAA+GGG	+	chr4.1:13267467-13267486	None:intergenic	55.0%
!!	TGCTGGAGCAGGTGTTGAAG+GGG	+	chr4.1:13267466-13267485	None:intergenic	55.0%
	GCCTTAGCAAGAGCTGGTGC+TGG	+	chr4.1:13268023-13268042	None:intergenic	60.0%
!!	GGAGCAGGTGTTGAAGGGGT+AGG	+	chr4.1:13267462-13267481	None:intergenic	60.0%
!!	GGGGTAGGTTTTGTAGGGGC+TGG	+	chr4.1:13267447-13267466	None:intergenic	60.0%
!!	GGTGCTGGAGCAGGTGTTGA+AGG	+	chr4.1:13267468-13267487	None:intergenic	60.0%
!!	GGTTTTGCTGGTGCTGGAGC+AGG	+	chr4.1:13267477-13267496	None:intergenic	60.0%
!!!	GGGGCTGGTTTTGTAGGTGC+TGG	+	chr4.1:13267432-13267451	None:intergenic	60.0%
!!!	GGTGCTTTTGGTGCTGCAGC+TGG	+	chr4.1:13267402-13267421	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	13267327	13268205	13267327	ID=MS.gene53259
chr4.1	mRNA	13267327	13268205	13267327	ID=MS.gene53259.t1;Parent=MS.gene53259
chr4.1	exon	13267327	13268205	13267327	ID=MS.gene53259.t1.exon1;Parent=MS.gene53259.t1
chr4.1	CDS	13267327	13268205	13267327	ID=cds.MS.gene53259.t1;Parent=MS.gene53259.t1

Gene Sequence

>MS.gene53259

ATGAATCAAATTTTCTTGTTCTCACTCCTACTACTTGCCTCTATCTTCTCAATCACCACTTTAGCTCAATCACCAGCTGCAGCACCAAAAGCACCAGAAAAACCAGCACCTACAAAACCAGCCCCTACAAAACCTACCCCTTCAACACCTGCTCCAGCACCAGCAAAACCATTAGTCCCAGCATTACCACAATCACCTACAACAAATCCTGATTCGTCAGGAAACCAAGACATAATCAAGATTCTAAGAAAAGCCAAATCATTCAACACACTAATCCGTTTGCTCAAGACAACACAAATCATCAACCAAGTCAATGCACAGCTTGTAACTACAAAAAATGGAGGCTTGACAATTCTTGCACCAGATGATGGTGCCTTTTCAGAACTCAAAGCAGGTTACTTCAACTCCTTGGGAGAAAGACAACAAAAAGAATTGATTCAGTATCATGTTCTTCCTGAATATGTATCAAGTTCGAACTTTGATTCTCTCAGCAACCCTGTGTTGACACTAGCTAGTGATAGCCCACAAGGATTTCAGATTAATGTGACTGCATATGGAAACAGTGTGAATATTTCAACTGGCGTTGTTGATACAACCATCACTGGAATCGTTTACGCTGATAAAACACTTGCTATATATCATGTGAATAAGGTTCTTATTCCTTTGGATTTTGTTAAGCCTAAAGCTAAAGCTCCAGCACCAGCTCTTGCTAAGGCACCTAAAGCTGAGAAAGAGAATTCATCTGCAGATGATGATGATCAGACTCAGGCACACAAGGATAGTTCTGATGCCATTAGCTTGAATAACATGCATGGAATATCAGTGTTGGTTTCCCTTGGAATTTCTCTGCTTGCAGTTGCAGTAACAACTATGTTGTGA

Protein sequence

>MS.gene53259.t1

MNQIFLFSLLLLASIFSITTLAQSPAAAPKAPEKPAPTKPAPTKPTPSTPAPAPAKPLVPALPQSPTTNPDSSGNQDIIKILRKAKSFNTLIRLLKTTQIINQVNAQLVTTKNGGLTILAPDDGAFSELKAGYFNSLGERQQKELIQYHVLPEYVSSSNFDSLSNPVLTLASDSPQGFQINVTAYGNSVNISTGVVDTTITGIVYADKTLAIYHVNKVLIPLDFVKPKAKAPAPALAKAPKAEKENSSADDDDQTQAHKDSSDAISLNNMHGISVLVSLGISLLAVAVTTML