AlfalfaGEDB | MS.gene53267

M. sativa cultivar XinJiangDaYe / MS.gene53267

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28 PF08263.15 PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53267.t1	XP_003629836.2	95.9	632	24	1	1	630	1	632	5.20E-293	1016.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53267.t1	Q9LJY0	48.5	602	290	7	28	613	51	648	1.6e-120	434.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53267.t1	G7L789	95.9	632	24	1	1	630	1	632	3.8e-293	1016.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058495	MS.gene53267	0.81397	1.96E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53267.t1	MTR_8g087420	97.152	632	16	1	1	630	1	632	0.0	1262
MS.gene53267.t1	MTR_1g009270	62.019	624	221	8	1	615	2	618	0.0	764
MS.gene53267.t1	MTR_8g101260	45.774	627	304	12	9	616	12	621	0.0	527
MS.gene53267.t1	MTR_6g093050	44.817	627	303	13	13	616	15	621	6.65e-174	509
MS.gene53267.t1	MTR_2g105900	44.462	632	295	10	24	613	2	619	5.10e-172	504
MS.gene53267.t1	MTR_2g042710	39.803	608	343	7	23	610	23	627	9.24e-149	444
MS.gene53267.t1	MTR_4g124990	39.040	625	354	10	10	612	7	626	5.67e-145	434
MS.gene53267.t1	MTR_8g021350	38.760	645	331	11	28	612	31	671	1.86e-144	436
MS.gene53267.t1	MTR_3g093710	36.699	624	360	19	8	612	8	615	9.74e-104	328
MS.gene53267.t1	MTR_1g061590	31.183	651	397	13	7	612	3	647	1.21e-100	321

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53267.t1	AT4G31250	44.495	654	306	11	1	611	1	640	0.0	536
MS.gene53267.t1	AT5G35390	44.637	634	313	12	7	615	12	632	4.83e-177	518
MS.gene53267.t1	AT3G20190	45.860	628	310	10	10	613	27	648	1.64e-174	512
MS.gene53267.t1	AT1G50610	44.268	628	308	9	27	619	45	665	7.04e-169	498
MS.gene53267.t1	AT2G07040	43.922	617	320	11	11	611	6	612	3.22e-168	495
MS.gene53267.t1	AT3G42880	37.224	634	356	13	11	611	7	631	5.61e-131	399
MS.gene53267.t1	AT5G20690	35.781	640	358	11	22	612	23	658	3.14e-126	387
MS.gene53267.t1	AT3G20190	48.905	411	193	6	216	613	53	459	1.48e-113	349
MS.gene53267.t1	AT1G72460	35.556	630	375	9	8	608	3	630	6.78e-113	352
MS.gene53267.t1	AT2G26730	34.603	630	373	16	24	629	23	637	3.83e-99	317

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Find 134 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACTCTGTAGAAAATC+TGG	0.391109	4.3:+13444320	None:intergenic
AAAGTCTAGGTAATTGAGCA+AGG	0.571288	4.3:+13444384	None:intergenic
AAATCAATATCATAAAGTCT+AGG	0.597803	4.3:+13444371	None:intergenic
AAATGAAGATTTGGCAATGT+GGG	0.597827	4.3:-13442908	MS.gene53267:CDS
AAATTACTTAAGGCATGGGA+AGG	0.597555	4.3:-13442935	MS.gene53267:CDS
AACACTAAAACTCTTCAAAT+TGG	0.276070	4.3:+13444586	None:intergenic
AACCCTTCAAAAGCATCATC+AGG	0.459040	4.3:+13444467	None:intergenic
AAGAGCGACGTATGGTGTTT+AGG	0.257578	4.3:-13442997	MS.gene53267:CDS
AAGGGTTGAGTTTGATTTAC+AGG	0.289643	4.3:-13443682	MS.gene53267:CDS
AAGTCTAGGTAATTGAGCAA+GGG	0.628160	4.3:+13444385	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGAAGATTTGGCAATGT+GGG	-	chr4.3:13444624-13444643	MS.gene53267:CDS	30.0%
ACACGTTTGAAGATAATCAA+AGG	-	chr4.3:13444298-13444317	MS.gene53267:CDS	30.0%
AGTCCATACTCAGTTAAAAA+AGG	+	chr4.3:13444427-13444446	None:intergenic	30.0%
AGTTCTATGATCATATGAGA+AGG	-	chr4.3:13444002-13444021	MS.gene53267:CDS	30.0%
ATAAGAACATGGTGTTTCTT+AGG	+	chr4.3:13443602-13443621	None:intergenic	30.0%
ATTCTATTAATGTGTGCACA+TGG	-	chr4.3:13442823-13442842	MS.gene53267:CDS	30.0%
CATAATAGAATGCAACTAGA+GGG	+	chr4.3:13444057-13444076	None:intergenic	30.0%
CATATCTAAAAATGGTTCCT+AGG	+	chr4.3:13443461-13443480	None:intergenic	30.0%
CCTGCATTCAATAAAATTGT+GGG	-	chr4.3:13442996-13443015	MS.gene53267:CDS	30.0%
CTGACCAAAAATAAATTCTC+TGG	-	chr4.3:13443035-13443054	MS.gene53267:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	13442669	13444885	13442669	ID=MS.gene53267
chr4.3	mRNA	13442669	13444885	13442669	ID=MS.gene53267.t1;Parent=MS.gene53267
chr4.3	exon	13444237	13444885	13444237	ID=MS.gene53267.t1.exon1;Parent=MS.gene53267.t1
chr4.3	CDS	13444237	13444885	13444237	ID=cds.MS.gene53267.t1;Parent=MS.gene53267.t1
chr4.3	exon	13443412	13444041	13443412	ID=MS.gene53267.t1.exon2;Parent=MS.gene53267.t1
chr4.3	CDS	13443412	13444041	13443412	ID=cds.MS.gene53267.t1;Parent=MS.gene53267.t1
chr4.3	exon	13442669	13443282	13442669	ID=MS.gene53267.t1.exon3;Parent=MS.gene53267.t1
chr4.3	CDS	13442669	13443282	13442669	ID=cds.MS.gene53267.t1;Parent=MS.gene53267.t1

Gene Sequence

>MS.gene53267

ATGTTTCATGAAAGAGAATACATCTCTATTATCATTCTCTTCATGATAGCCTCTTGTTTCTTACCATCCTCAACAGCTGACACAGATGCTCAAATTCTAGTGAACTTCAAAAGCTTCTTATCCAATGCAGATGCTTTAAACAATTGGAGTAACGATTCTATTAATGTGTGCACATGGACTGGTTTAATATGCATCAATCAAACTATACTTCATGGTTTGAGACTAGAAAATATGGGACTAAGTGGAACAATTAATGTAGACATCCTTATGAAATTGTCCAATTTGAAGAGTTTTAGTGTTATTAACAATAATTTTGAGGGTCCAATGCCTGCATTCAATAAAATTGTGGGATTAAGGGCATTGTTTCTGACCAAAAATAAATTCTCTGGTGAAATTCCTGATGATGCTTTTGAAGGGTTAAGATGGCTCAAGAGAGTATTCTTAGCAGAAAATGGATTTAAGGGTCATATTCCTAAATCCCTTGCTCAATTACCTAGACTTTATGATATTGATTTGCATGGAAATAGCTTTGATGGCAATATACCAGATTTTCTACAGAGTGGTTTCAGAGTGTTTGATTTATCTAACAATCAATTGGAGGGTGCAATACCAGAAGGTTTAAGAAATGAGGATCCAAGTTCTTTTGCAGGTATGCAATTAGTGTTTAATTTGTTCTTACTTATTGCTATGAGAAATTCAAAACAAATATCCAAATTTATTTTGAATTTATTCCGACTATAATCAATCTTTCAGATTTTCTTGGATTTGGATTGTTACTTTGTTCTTGTTCCTAGGAACCATTTTTAGATATGTTTTATTTTATCTAATATTCCTTGTGCAACAGGCAACAAAGGTCTGTGCGGAAAACCTCTTGAACAGCCCTGCAGCGAAAGCCATAGTGCTCCGCGTGAAGAAGAAAAAGAAAAAGAACCTAAGAAACACCATGTTCTTATTAGTATTATTGCATTTGTGGTAGTGCTTATATTGGCTTCAATACTAGCACTTTTGTACATCCGTTACCGTAGAAAGAAAGCTGCAGAAAAATCCATTTGGAATATGGAGAATGCACAATCACAGTCTCATAACACCAACACCAGTACAGTTTCCACTAGTGAGGCCAAATCAATTGTAGTAGAATCCAAGAAAAACAAAGATGAAGATTTGAACTTCGTTTCGAACGAAAGGGTTGAGTTTGATTTACAGGACTTGTTAAGGGCCTCAGCTGAGGTTTTAGGAAGTGGAAGTTTTGGATCCACTTACAAGGCTATGGTATTGACAGGACCAGTTGTTGTTGTGAAAAGATTTAAGCACATGAACAAAGTTGGCAAAAAAGAGTTCTATGATCATATGAGAAGGCTTGGAAGGTTGACACATCCTAATCTACTCCCTCTAGTTGCATTCTATTATGGAAAAGAAGAGAAGCTTTTGATACATGATTTTGCAGAAAATGGCAGCTTGGCTAGTCATCTTCACGGTATGATATGCCTACTTTAATTCATTTTATAATCTATATCATAAGAAACATCAAATGTAACTAGTAATTTTGATTGTGATTGTGCCAAATTATGCCTCGTGTTTATGTTGATTTGCATTATGAATGCAGGTAGACATTGTGAGTTGGATTGGGCAACACGTTTGAAGATAATCAAAGGAGTAGCTAGAGGTTTGGCTTACCTATACAGAGAGTTTCCGGACGAAAAGTTACCACATGGACATCTAAAATCTTCTAATGTTGTGTTAGACCATTCATTTGAGCCTTTTTTAACTGAGTATGGACTTGTGGAAGTCACTGACTTGAAGCATGCTCAACAGTTCATGGTAGGCTTCAAGTCCCCAGAGGTGAGTCAACATGAAGGGCCGAGCGAAAAGAGCGACGTATGGTGTTTAGGGATACTTATTTTGGAGCTATTGACAGGAAAGTTCCCTGCAAATTACTTAAGGCATGGGAAGGGAGCAAATGAAGATTTGGCAATGTGGGTGGAATCTATAGTGAGGGAAGGGTGGAGTGGAGAAGTGTTGGATAAAAGCATTGGTGGTGGGAGTAGAGGTGAAGAAGGTGAGATGTTGAAGCTGTTGAGGATTGGAATGAGTTGTTGTGAATGGAGTTTGGAGAATAGGTTGGGTTGGAAGGATGCAGTGGCAAAGATTGAGGAGTTGAAAGAAATGGATCATGTAGGGGTTGGAGTTCAAAGTCAAGACTCTGATTTGTCTCTTTGA

Protein sequence

>MS.gene53267.t1

MFHEREYISIIILFMIASCFLPSSTADTDAQILVNFKSFLSNADALNNWSNDSINVCTWTGLICINQTILHGLRLENMGLSGTINVDILMKLSNLKSFSVINNNFEGPMPAFNKIVGLRALFLTKNKFSGEIPDDAFEGLRWLKRVFLAENGFKGHIPKSLAQLPRLYDIDLHGNSFDGNIPDFLQSGFRVFDLSNNQLEGAIPEGLRNEDPSSFAGNKGLCGKPLEQPCSESHSAPREEEKEKEPKKHHVLISIIAFVVVLILASILALLYIRYRRKKAAEKSIWNMENAQSQSHNTNTSTVSTSEAKSIVVESKKNKDEDLNFVSNERVEFDLQDLLRASAEVLGSGSFGSTYKAMVLTGPVVVVKRFKHMNKVGKKEFYDHMRRLGRLTHPNLLPLVAFYYGKEEKLLIHDFAENGSLASHLHGRHCELDWATRLKIIKGVARGLAYLYREFPDEKLPHGHLKSSNVVLDHSFEPFLTEYGLVEVTDLKHAQQFMVGFKSPEVSQHEGPSEKSDVWCLGILILELLTGKFPANYLRHGKGANEDLAMWVESIVREGWSGEVLDKSIGGGSRGEEGEMLKLLRIGMSCCEWSLENRLGWKDAVAKIEELKEMDHVGVGVQSQDSDLSL