AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53273

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53273.t1 MTR_8g087460 95.886 316 12 1 1 315 1 316 0.0 528
MS.gene53273.t1 MTR_8g087470 95.570 316 13 1 1 315 1 316 0.0 525
MS.gene53273.t1 MTR_2g017950 62.460 309 73 8 15 312 15 291 1.15e-109 320
MS.gene53273.t1 MTR_4g059840 52.308 260 94 8 4 256 2 238 5.36e-72 224
MS.gene53273.t1 MTR_4g053380 51.504 266 102 9 6 265 6 250 5.12e-70 219
MS.gene53273.t1 MTR_4g059790 52.500 240 85 7 23 256 22 238 6.72e-68 214
MS.gene53273.t1 MTR_2g093970 43.137 306 143 8 13 313 10 289 2.45e-61 197
MS.gene53273.t1 MTR_2g017960 50.442 226 89 6 93 311 68 277 7.01e-61 195
MS.gene53273.t1 MTR_2g017970 51.570 223 90 6 93 314 68 273 7.99e-61 195
MS.gene53273.t1 MTR_4g059720 50.855 234 60 6 13 244 10 190 1.42e-60 194
MS.gene53273.t1 MTR_2g093980 51.754 228 93 7 55 281 42 253 3.99e-59 191
MS.gene53273.t1 MTR_2g093990 56.667 180 73 3 66 244 14 189 1.51e-57 186
MS.gene53273.t1 MTR_8g087450 51.802 222 93 7 22 242 16 224 5.82e-56 183
MS.gene53273.t1 MTR_4g059780 57.426 202 69 8 42 242 52 237 8.29e-55 181
MS.gene53273.t1 MTR_7g101080 50.000 158 72 4 87 242 35 187 4.97e-44 151
MS.gene53273.t1 MTR_2g090765 40.223 179 97 6 66 242 14 184 6.34e-29 111
MS.gene53273.t1 MTR_5g098060 31.395 172 114 2 92 263 47 214 3.67e-18 82.8
MS.gene53273.t1 MTR_7g075453 33.333 189 105 6 74 256 165 338 1.16e-15 77.4
MS.gene53273.t1 MTR_2g437700 30.769 156 97 4 92 242 194 343 4.18e-13 69.7
MS.gene53273.t1 MTR_5g098420 30.978 184 103 7 76 255 188 351 3.43e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53273.t1 AT5G03170 51.053 190 82 6 71 258 18 198 1.50e-52 173
MS.gene53273.t1 AT5G60490 56.376 149 59 3 93 239 39 183 3.58e-48 162
MS.gene53273.t1 AT2G20520 41.451 193 103 6 66 255 15 200 1.25e-31 119
MS.gene53273.t1 AT5G44130 40.120 167 93 4 92 255 35 197 3.16e-27 107
MS.gene53273.t1 AT1G03870 35.052 194 116 5 92 279 38 227 3.66e-25 101
MS.gene53273.t1 AT3G46550 32.544 169 103 4 92 255 206 368 3.63e-18 84.7
MS.gene53273.t1 AT3G60900 30.000 180 115 3 93 270 190 360 9.96e-12 65.9
MS.gene53273.t1 AT2G04780 30.667 150 96 4 92 239 45 188 2.03e-11 63.5
MS.gene53273.t1 AT2G04780 30.667 150 96 4 92 239 45 188 2.03e-11 63.5

Find 90 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCAATGGTGGGTCCTTT+TGG 0.235120 4.1:+13224412 None:intergenic
GCCTCTTGATCTTGTTCTTC+CGG 0.272392 4.1:-13223957 MS.gene53273:CDS
TAGCAAACTTGCTATTTATC+AGG 0.275231 4.1:-13223996 MS.gene53273:CDS
GGTGCTGTTGCTGTTGTTGC+TGG 0.314474 4.1:+13224508 None:intergenic
CCTGCTGATGTTTCTGCTGC+AGG 0.329762 4.1:-13223809 MS.gene53273:CDS
GTTGCTGTTGTTGCTGGTAA+TGG 0.333085 4.1:+13224514 None:intergenic
GAGGAACCCTGCTTTGAGTT+TGG 0.336926 4.1:+13224237 None:intergenic
GTTGGTGGAATTGTTCTATA+AGG 0.340654 4.1:-13223743 None:intergenic
AATTAGAACGGAAAACCTTT+TGG 0.343872 4.1:+13224369 None:intergenic
GGGTGGAATTGGTGAGAGTT+GGG 0.354472 4.1:+13224588 None:intergenic
TTGGAGAATGCAGAATCTTC+TGG 0.359282 4.1:+13224256 None:intergenic
GCAGTTAATGCTACCGTTAC+CGG 0.364782 4.1:-13224034 MS.gene53273:CDS
TCACCGGCTTGTGTTTGAAC+AGG 0.366430 4.1:+13224121 None:intergenic
GGGGTGGAATTGGTGAGAGT+TGG 0.379400 4.1:+13224587 None:intergenic
GTTAACCCTCCTGAACTTGA+TGG 0.380393 4.1:+13224289 None:intergenic
CGGTGGCAGTCAAGTTAGTA+TGG 0.391071 4.1:-13224065 MS.gene53273:CDS
TCTGGCTCCGGCGCCAGCTA+AGG 0.394000 4.1:-13223921 MS.gene53273:CDS
TGCTATTTATCAGGTTGATA+AGG 0.394299 4.1:-13223987 MS.gene53273:CDS
TGCTGCAGGGTCTGTGATGT+GGG 0.418106 4.1:-13223795 MS.gene53273:CDS
TGATCTTGTTCTTCCGGCTA+AGG 0.420269 4.1:-13223951 MS.gene53273:CDS
GTATCATCGCCATCAAGTTC+AGG 0.420444 4.1:-13224298 MS.gene53273:CDS
TCCGGCTAAGGCTCCATCTC+TGG 0.421914 4.1:-13223939 MS.gene53273:CDS
GGTGCACCAGTTGGTGTTGT+TGG 0.431617 4.1:+13224454 None:intergenic
GGTGATGGAGATGATGTTGT+AGG 0.435283 4.1:+13224565 None:intergenic
TGTTTGAACAGGGTTGGTTA+AGG 0.439720 4.1:+13224132 None:intergenic
GGGACTGTTATGGTGGGTGA+TGG 0.441052 4.1:+13224433 None:intergenic
AAGGGTTTGTTGCCTAAGGC+TGG 0.443081 4.1:-13223902 MS.gene53273:CDS
GGTGGTGATGCTGTTGTTGA+TGG 0.445870 4.1:+13224535 None:intergenic
TGATGCTGTTGTTGATGGTA+AGG 0.449757 4.1:+13224540 None:intergenic
CTGGCTCCGGCGCCAGCTAA+GGG 0.452323 4.1:-13223920 MS.gene53273:CDS
CTGCTGCAGGGTCTGTGATG+TGG 0.454235 4.1:-13223796 MS.gene53273:CDS
AAGAATGTTGATTATGTCAA+TGG 0.455371 4.1:+13224399 None:intergenic
CAACTGAACGTCACTACTTA+CGG 0.460289 4.1:-13224085 MS.gene53273:CDS
AGCTAAGGGTTTGTTGCCTA+AGG 0.460321 4.1:-13223906 MS.gene53273:CDS
GTGATGGAGATGATGTTGTA+GGG 0.461192 4.1:+13224566 None:intergenic
GCATTCTCCAAACTCAAAGC+AGG 0.466381 4.1:-13224244 MS.gene53273:CDS
CTTGTTCCAACAACACCAAC+TGG 0.476196 4.1:-13224460 MS.gene53273:CDS
CTGCTGATGTTTCTGCTGCA+GGG 0.487153 4.1:-13223808 MS.gene53273:CDS
TGGTGAGAGTTGGGCTAAAG+TGG 0.489699 4.1:+13224597 None:intergenic
TTAGGCAACAAACCCTTAGC+TGG 0.492766 4.1:+13223908 None:intergenic
GTCTATCAGTGAGGGAGTTG+AGG 0.498053 4.1:+13224218 None:intergenic
AACAAACCCTTAGCTGGCGC+CGG 0.504015 4.1:+13223914 None:intergenic
GGCTTGTGTTTGAACAGGGT+TGG 0.506531 4.1:+13224126 None:intergenic
CACCGGCTTGTGTTTGAACA+GGG 0.508119 4.1:+13224122 None:intergenic
GTAGACGATGGCAGTGGTGC+TGG 0.509847 4.1:-13223860 MS.gene53273:CDS
GCCAGAGATGGAGCCTTAGC+CGG 0.520251 4.1:+13223938 None:intergenic
GCTGGCGCCGGAGCCAGAGA+TGG 0.521879 4.1:+13223926 None:intergenic
TAAGGCTCCATCTCTGGCTC+CGG 0.530879 4.1:-13223933 MS.gene53273:CDS
AACGGTAGCATTAACTGCTC+CGG 0.540707 4.1:+13224039 None:intergenic
AGTGGAACTGTGCTTAATCC+TGG 0.544466 4.1:+13224481 None:intergenic
TGATGGAGATGATGTTGTAG+GGG 0.546507 4.1:+13224567 None:intergenic
TTTGGAATGAGTTTGGTTGG+TGG 0.550464 4.1:-13223758 MS.gene53273:CDS
AGTGGTGCTGGTAGTGATGA+TGG 0.554877 4.1:-13223848 MS.gene53273:CDS
GTTGGTGTTGTTGGAACAAG+TGG 0.555566 4.1:+13224463 None:intergenic
GTTGATGGTAAGGGTGGTGA+TGG 0.560733 4.1:+13224550 None:intergenic
CCCACCATAACAGTCCCAAA+AGG 0.562203 4.1:-13224427 MS.gene53273:CDS
ATAAATAGCAAGTTTGCTAT+CGG 0.562446 4.1:+13224000 None:intergenic
ATACTAGCTGCGAGTTGAGT+TGG 0.564006 4.1:+13224317 None:intergenic
GGTATAAACAGTACCGGTAA+CGG 0.565166 4.1:+13224021 None:intergenic
TTTGAGAAGCCGAATTAGAA+CGG 0.569636 4.1:+13224357 None:intergenic
GTGGGTGATGGTGCACCAGT+TGG 0.574265 4.1:+13224445 None:intergenic
AACCCTGTTCAAACACAAGC+CGG 0.576254 4.1:-13224124 MS.gene53273:CDS
AATTGCTAGAAGAAGAAATG+AGG 0.579432 4.1:+13224636 None:intergenic
GCTGTTGTTGATGGTAAGGG+TGG 0.581658 4.1:+13224544 None:intergenic
GCTGTTGTTGCTGGTAATGG+TGG 0.582499 4.1:+13224517 None:intergenic
TGCTAGAAGAAGAAATGAGG+TGG 0.583738 4.1:+13224639 None:intergenic
CACAAGCCGGTGATGATGCT+AGG 0.584174 4.1:-13224111 MS.gene53273:CDS
GATGGAGATGATGTTGTAGG+GGG 0.584616 4.1:+13224568 None:intergenic
GAGTTGGGCTAAAGTGGTGG+AGG 0.587512 4.1:+13224603 None:intergenic
TGAGAGTTGGGCTAAAGTGG+TGG 0.590350 4.1:+13224600 None:intergenic
TCGGTTGTAGACGATGGCAG+TGG 0.591876 4.1:-13223866 MS.gene53273:CDS
ACAGCAACAGCACCATCACC+AGG 0.599988 4.1:-13224499 MS.gene53273:CDS
AGTCAAGTTAGTATGGCTAC+CGG 0.601147 4.1:-13224058 MS.gene53273:CDS
ACTGTGCTTAATCCTGGTGA+TGG 0.604248 4.1:+13224487 None:intergenic
GATGCTGTTGTTGATGGTAA+GGG 0.606751 4.1:+13224541 None:intergenic
GCTATCGGTATAAACAGTAC+CGG 0.606873 4.1:+13224015 None:intergenic
CATTCTCCAAACTCAAAGCA+GGG 0.610410 4.1:-13224243 MS.gene53273:CDS
GCCGGAAGAACAAGATCAAG+AGG 0.613702 4.1:+13223956 None:intergenic
CCTGCAGCAGAAACATCAGC+AGG 0.614736 4.1:+13223809 None:intergenic
AATGTTGATTATGTCAATGG+TGG 0.616748 4.1:+13224402 None:intergenic
TTGAAGCCTAGCATCATCAC+CGG 0.621788 4.1:+13224105 None:intergenic
TCATCGCCATCAAGTTCAGG+AGG 0.622735 4.1:-13224295 MS.gene53273:CDS
AAATCGAAACGAATGAAGCG+AGG 0.637672 4.1:+13224167 None:intergenic
AATCGAAACGAATGAAGCGA+GGG 0.648536 4.1:+13224168 None:intergenic
TAGTGATGATGGTGATGGCA+AGG 0.668905 4.1:-13223837 MS.gene53273:CDS
AATTCATCGGTTGTAGACGA+TGG 0.674617 4.1:-13223872 MS.gene53273:CDS
GCTGGTAGTGATGATGGTGA+TGG 0.674633 4.1:-13223842 MS.gene53273:CDS
ATGTTGATTATGTCAATGGT+GGG 0.681774 4.1:+13224403 None:intergenic
CATCGCCATCAAGTTCAGGA+GGG 0.702614 4.1:-13224294 MS.gene53273:CDS
CTGAACGTCACTACTTACGG+TGG 0.722980 4.1:-13224082 MS.gene53273:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATGTTGATTATGTCAA+TGG + chr4.1:13224019-13224038 None:intergenic 25.0%
!!! ATAAATAGCAAGTTTGCTAT+CGG + chr4.1:13224418-13224437 None:intergenic 25.0%
AATTGCTAGAAGAAGAAATG+AGG + chr4.1:13223782-13223801 None:intergenic 30.0%
ACATTCTTCAAAAAGCCAAA+AGG - chr4.1:13224031-13224050 MS.gene53273:CDS 30.0%
! AAAGGTTTTCCGTTCTAATT+CGG - chr4.1:13224049-13224068 MS.gene53273:CDS 30.0%
! AACTTTTTGTCTATCAGTGA+GGG + chr4.1:13224208-13224227 None:intergenic 30.0%
! AATGTTGATTATGTCAATGG+TGG + chr4.1:13224016-13224035 None:intergenic 30.0%
! AATTAGAACGGAAAACCTTT+TGG + chr4.1:13224049-13224068 None:intergenic 30.0%
! ATGTTGATTATGTCAATGGT+GGG + chr4.1:13224015-13224034 None:intergenic 30.0%
!! TAGCAAACTTGCTATTTATC+AGG - chr4.1:13224419-13224438 MS.gene53273:CDS 30.0%
!! TGCTATTTATCAGGTTGATA+AGG - chr4.1:13224428-13224447 MS.gene53273:CDS 30.0%
!!! TGTTGTTTTTGGAATGAGTT+TGG - chr4.1:13224650-13224669 MS.gene53273:CDS 30.0%
GGCTGGAAAAACTAATTCAT+CGG - chr4.1:13224530-13224549 MS.gene53273:CDS 35.0%
TTTGAGAAGCCGAATTAGAA+CGG + chr4.1:13224061-13224080 None:intergenic 35.0%
! CAACTTTTTGTCTATCAGTG+AGG + chr4.1:13224209-13224228 None:intergenic 35.0%
!!! GAATTAGTTTTTCCAGCCTT+AGG + chr4.1:13224528-13224547 None:intergenic 35.0%
!!! GTTTTTGGAATGAGTTTGGT+TGG - chr4.1:13224654-13224673 MS.gene53273:CDS 35.0%
!!! TGGGTGAATGTTGTTGTTTT+TGG - chr4.1:13224639-13224658 MS.gene53273:CDS 35.0%
AAATCGAAACGAATGAAGCG+AGG + chr4.1:13224251-13224270 None:intergenic 40.0%
AATCGAAACGAATGAAGCGA+GGG + chr4.1:13224250-13224269 None:intergenic 40.0%
AGTCAAGTTAGTATGGCTAC+CGG - chr4.1:13224357-13224376 MS.gene53273:CDS 40.0%
CAACTGAACGTCACTACTTA+CGG - chr4.1:13224330-13224349 MS.gene53273:CDS 40.0%
CATTCTCCAAACTCAAAGCA+GGG - chr4.1:13224172-13224191 MS.gene53273:CDS 40.0%
GCTATCGGTATAAACAGTAC+CGG + chr4.1:13224403-13224422 None:intergenic 40.0%
TGATGGAGATGATGTTGTAG+GGG + chr4.1:13223851-13223870 None:intergenic 40.0%
TGCTAGAAGAAGAAATGAGG+TGG + chr4.1:13223779-13223798 None:intergenic 40.0%
TTGGAGAATGCAGAATCTTC+TGG + chr4.1:13224162-13224181 None:intergenic 40.0%
! AATTCATCGGTTGTAGACGA+TGG - chr4.1:13224543-13224562 MS.gene53273:CDS 40.0%
! GTGATGGAGATGATGTTGTA+GGG + chr4.1:13223852-13223871 None:intergenic 40.0%
! TTTGGAATGAGTTTGGTTGG+TGG - chr4.1:13224657-13224676 MS.gene53273:CDS 40.0%
!! GATGCTGTTGTTGATGGTAA+GGG + chr4.1:13223877-13223896 None:intergenic 40.0%
!! GGTATAAACAGTACCGGTAA+CGG + chr4.1:13224397-13224416 None:intergenic 40.0%
!! TGATGCTGTTGTTGATGGTA+AGG + chr4.1:13223878-13223897 None:intergenic 40.0%
!! TGTTTGAACAGGGTTGGTTA+AGG + chr4.1:13224286-13224305 None:intergenic 40.0%
AACCCTGTTCAAACACAAGC+CGG - chr4.1:13224291-13224310 MS.gene53273:CDS 45.0%
AACGGTAGCATTAACTGCTC+CGG + chr4.1:13224379-13224398 None:intergenic 45.0%
ACTGTGCTTAATCCTGGTGA+TGG + chr4.1:13223931-13223950 None:intergenic 45.0%
AGTGGAACTGTGCTTAATCC+TGG + chr4.1:13223937-13223956 None:intergenic 45.0%
CTTGTTCCAACAACACCAAC+TGG - chr4.1:13223955-13223974 MS.gene53273:CDS 45.0%
GATGGAGATGATGTTGTAGG+GGG + chr4.1:13223850-13223869 None:intergenic 45.0%
GCAGTTAATGCTACCGTTAC+CGG - chr4.1:13224381-13224400 MS.gene53273:CDS 45.0%
GCATTCTCCAAACTCAAAGC+AGG - chr4.1:13224171-13224190 MS.gene53273:CDS 45.0%
GCCTCTTGATCTTGTTCTTC+CGG - chr4.1:13224458-13224477 MS.gene53273:CDS 45.0%
GTATCATCGCCATCAAGTTC+AGG - chr4.1:13224117-13224136 MS.gene53273:CDS 45.0%
GTTAACCCTCCTGAACTTGA+TGG + chr4.1:13224129-13224148 None:intergenic 45.0%
TGATCTTGTTCTTCCGGCTA+AGG - chr4.1:13224464-13224483 MS.gene53273:CDS 45.0%
TTAGGCAACAAACCCTTAGC+TGG + chr4.1:13224510-13224529 None:intergenic 45.0%
TTGAAGCCTAGCATCATCAC+CGG + chr4.1:13224313-13224332 None:intergenic 45.0%
! ATACTAGCTGCGAGTTGAGT+TGG + chr4.1:13224101-13224120 None:intergenic 45.0%
! GTTGCTGTTGTTGCTGGTAA+TGG + chr4.1:13223904-13223923 None:intergenic 45.0%
!! AGCTAAGGGTTTGTTGCCTA+AGG - chr4.1:13224509-13224528 MS.gene53273:CDS 45.0%
!! GGTGATGGAGATGATGTTGT+AGG + chr4.1:13223853-13223872 None:intergenic 45.0%
!! GTTGGTGTTGTTGGAACAAG+TGG + chr4.1:13223955-13223974 None:intergenic 45.0%
!! TAGTGATGATGGTGATGGCA+AGG - chr4.1:13224578-13224597 MS.gene53273:CDS 45.0%
!!! ATGTCAATGGTGGGTCCTTT+TGG + chr4.1:13224006-13224025 None:intergenic 45.0%
!!! CCTTTTGGGACTGTTATGGT+GGG + chr4.1:13223991-13224010 None:intergenic 45.0%
!!! TCCTTTTGGGACTGTTATGG+TGG + chr4.1:13223992-13224011 None:intergenic 45.0%
!!! TGTCAATGGTGGGTCCTTTT+GGG + chr4.1:13224005-13224024 None:intergenic 45.0%
CATCGCCATCAAGTTCAGGA+GGG - chr4.1:13224121-13224140 MS.gene53273:CDS 50.0%
CCCACCATAACAGTCCCAAA+AGG - chr4.1:13223988-13224007 MS.gene53273:CDS 50.0%
CGGTGGCAGTCAAGTTAGTA+TGG - chr4.1:13224350-13224369 MS.gene53273:CDS 50.0%
CTGAACGTCACTACTTACGG+TGG - chr4.1:13224333-13224352 MS.gene53273:CDS 50.0%
GATGTTGTAGGGGGTGGAAT+TGG + chr4.1:13223841-13223860 None:intergenic 50.0%
GCCGGAAGAACAAGATCAAG+AGG + chr4.1:13224462-13224481 None:intergenic 50.0%
GGCTTGTGTTTGAACAGGGT+TGG + chr4.1:13224292-13224311 None:intergenic 50.0%
GGGTGGAATTGGTGAGAGTT+GGG + chr4.1:13223830-13223849 None:intergenic 50.0%
GTCTATCAGTGAGGGAGTTG+AGG + chr4.1:13224200-13224219 None:intergenic 50.0%
TCATCGCCATCAAGTTCAGG+AGG - chr4.1:13224120-13224139 MS.gene53273:CDS 50.0%
TGAGAGTTGGGCTAAAGTGG+TGG + chr4.1:13223818-13223837 None:intergenic 50.0%
TGGTGAGAGTTGGGCTAAAG+TGG + chr4.1:13223821-13223840 None:intergenic 50.0%
! CTGCTGATGTTTCTGCTGCA+GGG - chr4.1:13224607-13224626 MS.gene53273:CDS 50.0%
! GAGGAACCCTGCTTTGAGTT+TGG + chr4.1:13224181-13224200 None:intergenic 50.0%
! GGTGGTGATGCTGTTGTTGA+TGG + chr4.1:13223883-13223902 None:intergenic 50.0%
!! AAGGGTTTGTTGCCTAAGGC+TGG - chr4.1:13224513-13224532 MS.gene53273:CDS 50.0%
!! AGTGGTGCTGGTAGTGATGA+TGG - chr4.1:13224567-13224586 MS.gene53273:CDS 50.0%
!! CACCGGCTTGTGTTTGAACA+GGG + chr4.1:13224296-13224315 None:intergenic 50.0%
!! GCTGGTAGTGATGATGGTGA+TGG - chr4.1:13224573-13224592 MS.gene53273:CDS 50.0%
!! GCTGTTGTTGATGGTAAGGG+TGG + chr4.1:13223874-13223893 None:intergenic 50.0%
!! GCTGTTGTTGCTGGTAATGG+TGG + chr4.1:13223901-13223920 None:intergenic 50.0%
!! GTTGATGGTAAGGGTGGTGA+TGG + chr4.1:13223868-13223887 None:intergenic 50.0%
!! TCACCGGCTTGTGTTTGAAC+AGG + chr4.1:13224297-13224316 None:intergenic 50.0%
!!! GGGTCCTTTTGGGACTGTTA+TGG + chr4.1:13223995-13224014 None:intergenic 50.0%
AACAAACCCTTAGCTGGCGC+CGG + chr4.1:13224504-13224523 None:intergenic 55.0%
ACAGCAACAGCACCATCACC+AGG - chr4.1:13223916-13223935 MS.gene53273:CDS 55.0%
CCTGCAGCAGAAACATCAGC+AGG + chr4.1:13224609-13224628 None:intergenic 55.0%
GAGTTGGGCTAAAGTGGTGG+AGG + chr4.1:13223815-13223834 None:intergenic 55.0%
GGAGATGATGTTGTAGGGGG+TGG + chr4.1:13223847-13223866 None:intergenic 55.0%
GGGGTGGAATTGGTGAGAGT+TGG + chr4.1:13223831-13223850 None:intergenic 55.0%
TCGGTTGTAGACGATGGCAG+TGG - chr4.1:13224549-13224568 MS.gene53273:CDS 55.0%
TGCTGCAGGGTCTGTGATGT+GGG - chr4.1:13224620-13224639 MS.gene53273:CDS 55.0%
! CACAAGCCGGTGATGATGCT+AGG - chr4.1:13224304-13224323 MS.gene53273:CDS 55.0%
! CCTGCTGATGTTTCTGCTGC+AGG - chr4.1:13224606-13224625 MS.gene53273:CDS 55.0%
! TAAGGCTCCATCTCTGGCTC+CGG - chr4.1:13224482-13224501 MS.gene53273:CDS 55.0%
!! GGGACTGTTATGGTGGGTGA+TGG + chr4.1:13223985-13224004 None:intergenic 55.0%
!! GGTGCACCAGTTGGTGTTGT+TGG + chr4.1:13223964-13223983 None:intergenic 55.0%
!! GGTGCTGTTGCTGTTGTTGC+TGG + chr4.1:13223910-13223929 None:intergenic 55.0%
CTGCTGCAGGGTCTGTGATG+TGG - chr4.1:13224619-13224638 MS.gene53273:CDS 60.0%
GCCAGAGATGGAGCCTTAGC+CGG + chr4.1:13224480-13224499 None:intergenic 60.0%
GTAGACGATGGCAGTGGTGC+TGG - chr4.1:13224555-13224574 MS.gene53273:CDS 60.0%
TCCGGCTAAGGCTCCATCTC+TGG - chr4.1:13224476-13224495 MS.gene53273:CDS 60.0%
! GTGGGTGATGGTGCACCAGT+TGG + chr4.1:13223973-13223992 None:intergenic 60.0%
CTGGCTCCGGCGCCAGCTAA+GGG - chr4.1:13224495-13224514 MS.gene53273:CDS 70.0%
! TCTGGCTCCGGCGCCAGCTA+AGG - chr4.1:13224494-13224513 MS.gene53273:CDS 70.0%
! GCTGGCGCCGGAGCCAGAGA+TGG + chr4.1:13224492-13224511 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13223745 13224692 13223745 ID=MS.gene53273
chr4.1 mRNA 13223745 13224692 13223745 ID=MS.gene53273.t1;Parent=MS.gene53273
chr4.1 exon 13223745 13224692 13223745 ID=MS.gene53273.t1.exon1;Parent=MS.gene53273.t1
chr4.1 CDS 13223745 13224692 13223745 ID=cds.MS.gene53273.t1;Parent=MS.gene53273.t1
Gene Sequence

>MS.gene53273

ATGAGGAATATCATGCAACAATCTCTCATTTCCACCTCATTTCTTCTTCTAGCAATTACTTTCTACACCTCCACCACTTTAGCCCAACTCTCACCAATTCCACCCCCTACAACATCATCTCCATCACCACCCTTACCATCAACAACAGCATCACCACCATTACCAGCAACAACAGCAACAGCACCATCACCAGGATTAAGCACAGTTCCACTTGTTCCAACAACACCAACTGGTGCACCATCACCCACCATAACAGTCCCAAAAGGACCCACCATTGACATAATCAACATTCTTCAAAAAGCCAAAAGGTTTTCCGTTCTAATTCGGCTTCTCAAAACAACACAATTAATCAACCAACTCAACTCGCAGCTAGTATCATCGCCATCAAGTTCAGGAGGGTTAACAATTTTCGCACCAGAAGATTCTGCATTCTCCAAACTCAAAGCAGGGTTCCTCAACTCCCTCACTGATAGACAAAAAGTTGAACTCTTACAGTTTCACTCCCTCGCTTCATTCGTTTCGATTTCAAACTTCGATACCTTAACCAACCCTGTTCAAACACAAGCCGGTGATGATGCTAGGCTTCAACTGAACGTCACTACTTACGGTGGCAGTCAAGTTAGTATGGCTACCGGAGCAGTTAATGCTACCGTTACCGGTACTGTTTATACCGATAGCAAACTTGCTATTTATCAGGTTGATAAGGTACTTATGCCTCTTGATCTTGTTCTTCCGGCTAAGGCTCCATCTCTGGCTCCGGCGCCAGCTAAGGGTTTGTTGCCTAAGGCTGGAAAAACTAATTCATCGGTTGTAGACGATGGCAGTGGTGCTGGTAGTGATGATGGTGATGGCAAGGATTTACCTGCTGATGTTTCTGCTGCAGGGTCTGTGATGTGGGTGAATGTTGTTGTTTTTGGAATGAGTTTGGTTGGTGGAATTGTTCTATAA
Protein sequence

>MS.gene53273.t1

MRNIMQQSLISTSFLLLAITFYTSTTLAQLSPIPPPTTSSPSPPLPSTTASPPLPATTATAPSPGLSTVPLVPTTPTGAPSPTITVPKGPTIDIINILQKAKRFSVLIRLLKTTQLINQLNSQLVSSPSSSGGLTIFAPEDSAFSKLKAGFLNSLTDRQKVELLQFHSLASFVSISNFDTLTNPVQTQAGDDARLQLNVTTYGGSQVSMATGAVNATVTGTVYTDSKLAIYQVDKVLMPLDLVLPAKAPSLAPAPAKGLLPKAGKTNSSVVDDGSGAGSDDGDGKDLPADVSAAGSVMWVNVVVFGMSLVGGIVL