Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53274.t1 | GAU18803.1 | 88.6 | 236 | 21 | 5 | 48 | 280 | 82 | 314 | 2.50E-98 | 368.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53274.t1 | Q8LEE9 | 56.2 | 169 | 66 | 5 | 45 | 210 | 26 | 189 | 1.1e-38 | 161.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53274.t1 | A0A2Z6M9N0 | 88.6 | 236 | 21 | 5 | 48 | 280 | 82 | 314 | 1.8e-98 | 368.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056470 | MS.gene53274 | 0.914271 | 2.33E-84 | -1.69E-46 |
| MS.gene058848 | MS.gene53274 | 0.889465 | 2.36E-73 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53274.t1 | MTR_8g087460 | 95.200 | 250 | 11 | 1 | 32 | 280 | 67 | 316 | 2.83e-155 | 436 |
| MS.gene53274.t1 | MTR_8g087470 | 95.600 | 250 | 10 | 1 | 32 | 280 | 67 | 316 | 6.71e-155 | 434 |
| MS.gene53274.t1 | MTR_2g017950 | 68.093 | 257 | 60 | 7 | 32 | 277 | 46 | 291 | 5.39e-99 | 292 |
| MS.gene53274.t1 | MTR_4g059840 | 57.005 | 207 | 75 | 7 | 36 | 234 | 53 | 253 | 1.58e-66 | 209 |
| MS.gene53274.t1 | MTR_4g059790 | 49.402 | 251 | 101 | 9 | 36 | 280 | 53 | 283 | 8.03e-66 | 207 |
| MS.gene53274.t1 | MTR_4g053380 | 58.000 | 200 | 73 | 7 | 36 | 230 | 57 | 250 | 1.26e-64 | 204 |
| MS.gene53274.t1 | MTR_2g017970 | 59.563 | 183 | 65 | 5 | 58 | 239 | 68 | 242 | 5.66e-62 | 197 |
| MS.gene53274.t1 | MTR_4g059720 | 62.874 | 167 | 56 | 4 | 44 | 209 | 29 | 190 | 7.66e-62 | 196 |
| MS.gene53274.t1 | MTR_2g017960 | 52.232 | 224 | 90 | 5 | 58 | 280 | 68 | 275 | 8.24e-62 | 196 |
| MS.gene53274.t1 | MTR_2g093980 | 57.062 | 177 | 64 | 4 | 32 | 207 | 51 | 216 | 3.03e-58 | 187 |
| MS.gene53274.t1 | MTR_2g093990 | 54.359 | 195 | 82 | 4 | 16 | 209 | 1 | 189 | 5.48e-58 | 186 |
| MS.gene53274.t1 | MTR_2g093970 | 42.177 | 294 | 140 | 12 | 4 | 277 | 5 | 288 | 4.02e-57 | 185 |
| MS.gene53274.t1 | MTR_8g087450 | 56.627 | 166 | 60 | 4 | 51 | 207 | 62 | 224 | 4.06e-55 | 180 |
| MS.gene53274.t1 | MTR_4g059780 | 60.116 | 173 | 60 | 5 | 36 | 207 | 73 | 237 | 4.29e-54 | 177 |
| MS.gene53274.t1 | MTR_7g101080 | 51.266 | 158 | 70 | 4 | 52 | 207 | 35 | 187 | 8.21e-46 | 155 |
| MS.gene53274.t1 | MTR_2g090765 | 42.775 | 173 | 90 | 5 | 36 | 207 | 20 | 184 | 7.04e-30 | 113 |
| MS.gene53274.t1 | MTR_5g098060 | 31.977 | 172 | 113 | 2 | 57 | 228 | 47 | 214 | 1.58e-19 | 85.9 |
| MS.gene53274.t1 | MTR_7g075453 | 32.323 | 198 | 111 | 6 | 39 | 226 | 165 | 349 | 1.87e-17 | 82.0 |
| MS.gene53274.t1 | MTR_2g437700 | 30.769 | 156 | 97 | 4 | 57 | 207 | 194 | 343 | 6.31e-14 | 71.6 |
| MS.gene53274.t1 | MTR_5g098420 | 31.148 | 183 | 102 | 7 | 41 | 219 | 188 | 350 | 1.86e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53274.t1 | AT5G03170 | 51.579 | 190 | 81 | 6 | 36 | 223 | 18 | 198 | 3.33e-54 | 176 |
| MS.gene53274.t1 | AT5G60490 | 57.718 | 149 | 57 | 3 | 58 | 204 | 39 | 183 | 1.90e-49 | 164 |
| MS.gene53274.t1 | AT2G20520 | 41.361 | 191 | 102 | 6 | 33 | 220 | 17 | 200 | 2.62e-32 | 119 |
| MS.gene53274.t1 | AT5G44130 | 40.719 | 167 | 92 | 4 | 57 | 220 | 35 | 197 | 8.74e-29 | 110 |
| MS.gene53274.t1 | AT1G03870 | 34.842 | 221 | 123 | 6 | 57 | 275 | 38 | 239 | 1.75e-28 | 109 |
| MS.gene53274.t1 | AT3G46550 | 31.383 | 188 | 118 | 4 | 40 | 222 | 189 | 370 | 3.18e-18 | 84.3 |
| MS.gene53274.t1 | AT3G60900 | 30.928 | 194 | 119 | 4 | 44 | 235 | 180 | 360 | 3.32e-14 | 72.4 |
| MS.gene53274.t1 | AT2G04780 | 31.333 | 150 | 95 | 4 | 57 | 204 | 45 | 188 | 3.97e-12 | 65.1 |
| MS.gene53274.t1 | AT2G04780 | 31.333 | 150 | 95 | 4 | 57 | 204 | 45 | 188 | 3.97e-12 | 65.1 |
Find 68 sgRNAs with CRISPR-Local
Find 175 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTCAATGGTGGGTCCTTT+TGG | 0.235120 | 4.1:+13221094 | None:intergenic |
| GCCTCTTGATCTTGTTCTTC+CGG | 0.272392 | 4.1:-13220639 | MS.gene53274:CDS |
| TAGCAAACTTGCTATTTATC+AGG | 0.275231 | 4.1:-13220678 | MS.gene53274:CDS |
| GAAATCATTGAACTTAACTA+TGG | 0.283444 | 4.1:-13222435 | MS.gene53274:CDS |
| GAGGAACCCTGCTTTGAGTT+TGG | 0.336926 | 4.1:+13220919 | None:intergenic |
| GCAGTTAATGCTACCGTTAC+TGG | 0.358591 | 4.1:-13220716 | MS.gene53274:CDS |
| TTGGAGAATGCAGAATCTTC+TGG | 0.359282 | 4.1:+13220938 | None:intergenic |
| GTTGGTGGAGTTGTTCTATA+AGG | 0.359667 | 4.1:-13220425 | None:intergenic |
| CCTGCTGATGTTTCTGCTGC+AGG | 0.360987 | 4.1:-13220491 | MS.gene53274:CDS |
| TTTGGCTCCTGCACCGGCTA+AGG | 0.362528 | 4.1:-13220603 | MS.gene53274:CDS |
| CAGTGGCAATCAAGTTAGTA+TGG | 0.363282 | 4.1:-13220747 | MS.gene53274:CDS |
| TCCGGCTAAGGCTCCAGCTT+TGG | 0.363612 | 4.1:-13220621 | MS.gene53274:CDS |
| TCACCGGCTTGTGTTTGAAC+AGG | 0.366430 | 4.1:+13220803 | None:intergenic |
| TGCTATTTATCAGGTTGATA+AGG | 0.394299 | 4.1:-13220669 | MS.gene53274:CDS |
| AATTAGAACGGAGAACCTTT+TGG | 0.395304 | 4.1:+13221051 | None:intergenic |
| TTTGGAATGGGTTTGGTTGG+TGG | 0.406116 | 4.1:-13220440 | MS.gene53274:CDS |
| GGTGCACCAGTTGGTGTTGT+TGG | 0.418778 | 4.1:+13221136 | None:intergenic |
| TGATCTTGTTCTTCCGGCTA+AGG | 0.420269 | 4.1:-13220633 | MS.gene53274:CDS |
| GCCGGTGCAGGAGCCAAAGC+TGG | 0.431263 | 4.1:+13220608 | None:intergenic |
| TGTTTGAACAGGGTTGGTTA+AGG | 0.439720 | 4.1:+13220814 | None:intergenic |
| CTGCTGCAGGATCTGTGCAT+TGG | 0.442652 | 4.1:-13220478 | MS.gene53274:CDS |
| AAGGGTTTGTTGCCTAAGGC+TGG | 0.443081 | 4.1:-13220584 | MS.gene53274:CDS |
| GTATCATCACCATCAGGTTC+AGG | 0.443291 | 4.1:-13220980 | MS.gene53274:CDS |
| CTTGTTCCAACAACACCAAC+TGG | 0.444147 | 4.1:-13221142 | MS.gene53274:CDS |
| AAGAATGTTGATTATGTCAA+TGG | 0.455371 | 4.1:+13221081 | None:intergenic |
| TTGGCTCCTGCACCGGCTAA+GGG | 0.463641 | 4.1:-13220602 | MS.gene53274:CDS |
| AACAAACCCTTAGCCGGTGC+AGG | 0.463951 | 4.1:+13220596 | None:intergenic |
| GCATTCTCCAAACTCAAAGC+AGG | 0.466381 | 4.1:-13220926 | MS.gene53274:CDS |
| GGCTAAGGGTTTGTTGCCTA+AGG | 0.468336 | 4.1:-13220588 | MS.gene53274:CDS |
| AATTCTTCGGTTGTAGATGA+TGG | 0.470488 | 4.1:-13220554 | MS.gene53274:CDS |
| GTAGATGATGGTAGCGGTGC+TGG | 0.479324 | 4.1:-13220542 | MS.gene53274:CDS |
| AAATCATTGAACTTAACTAT+GGG | 0.487536 | 4.1:-13222434 | MS.gene53274:CDS |
| TCCAGCTTTGGCTCCTGCAC+CGG | 0.487588 | 4.1:-13220609 | MS.gene53274:CDS |
| GTCTATCAGTGAGGGAGTTG+AGG | 0.498053 | 4.1:+13220900 | None:intergenic |
| GGCTTGTGTTTGAACAGGGT+TGG | 0.506531 | 4.1:+13220808 | None:intergenic |
| CACCGGCTTGTGTTTGAACA+GGG | 0.508119 | 4.1:+13220804 | None:intergenic |
| ATACTAGCTGCGAGTTGAGT+TGG | 0.509205 | 4.1:+13220999 | None:intergenic |
| GCCAAAGCTGGAGCCTTAGC+CGG | 0.517768 | 4.1:+13220620 | None:intergenic |
| AACGGTAGCATTAACTGCTC+CGG | 0.527378 | 4.1:+13220721 | None:intergenic |
| CAGCTAGTATCATCACCATC+AGG | 0.530331 | 4.1:-13220986 | MS.gene53274:CDS |
| CCCACCAAAGCAATCCCAAA+AGG | 0.532318 | 4.1:-13221109 | MS.gene53274:CDS |
| AAATAGAAACAAACGAAGCA+AGG | 0.540997 | 4.1:+13220849 | None:intergenic |
| AGCGGTGCTGGCAGTGATGA+TGG | 0.560075 | 4.1:-13220530 | MS.gene53274:CDS |
| TTAGGCAACAAACCCTTAGC+CGG | 0.560846 | 4.1:+13220590 | None:intergenic |
| ATAAATAGCAAGTTTGCTAT+CGG | 0.562446 | 4.1:+13220682 | None:intergenic |
| GTTGGTGTTGTTGGAACAAG+CGG | 0.574823 | 4.1:+13221145 | None:intergenic |
| AACCCTGTTCAAACACAAGC+CGG | 0.576254 | 4.1:-13220806 | MS.gene53274:CDS |
| CACAAGCCGGTGATGATGCT+AGG | 0.584174 | 4.1:-13220793 | MS.gene53274:CDS |
| AATCAAGTTAGTATGGCTAC+CGG | 0.593663 | 4.1:-13220740 | MS.gene53274:CDS |
| TCGGTTGTAGATGATGGTAG+CGG | 0.599850 | 4.1:-13220548 | MS.gene53274:CDS |
| TCACGAATCTTGCAAGTCAG+AGG | 0.604969 | 4.1:+13222401 | None:intergenic |
| CCTGCAGCAGAAACATCAGC+AGG | 0.607919 | 4.1:+13220491 | None:intergenic |
| TCATCACCATCAGGTTCAGG+AGG | 0.610251 | 4.1:-13220977 | MS.gene53274:CDS |
| CATTCTCCAAACTCAAAGCA+GGG | 0.610410 | 4.1:-13220925 | MS.gene53274:CDS |
| TTTGAGAAGACGAATTAGAA+CGG | 0.612652 | 4.1:+13221039 | None:intergenic |
| GCCGGAAGAACAAGATCAAG+AGG | 0.613702 | 4.1:+13220638 | None:intergenic |
| AATGTTGATTATGTCAATGG+TGG | 0.616748 | 4.1:+13221084 | None:intergenic |
| AATAGAAACAAACGAAGCAA+GGG | 0.620497 | 4.1:+13220850 | None:intergenic |
| TTGAAGCCTAGCATCATCAC+CGG | 0.621788 | 4.1:+13220787 | None:intergenic |
| CAGTGATGATGGTGATGGAA+AGG | 0.627687 | 4.1:-13220519 | MS.gene53274:CDS |
| CATTGAACTTAACTATGGGG+CGG | 0.636341 | 4.1:-13222430 | MS.gene53274:CDS |
| GGTATAAACAGTACCAGTAA+CGG | 0.640082 | 4.1:+13220703 | None:intergenic |
| GCTGGCAGTGATGATGGTGA+TGG | 0.645590 | 4.1:-13220524 | MS.gene53274:CDS |
| GTTAACCCTCCTGAACCTGA+TGG | 0.664853 | 4.1:+13220971 | None:intergenic |
| CTGAACGTCACTACTTACAG+TGG | 0.677815 | 4.1:-13220764 | MS.gene53274:CDS |
| AATCATTGAACTTAACTATG+GGG | 0.681123 | 4.1:-13222433 | MS.gene53274:CDS |
| ATGTTGATTATGTCAATGGT+GGG | 0.681774 | 4.1:+13221085 | None:intergenic |
| CATCACCATCAGGTTCAGGA+GGG | 0.700637 | 4.1:-13220976 | MS.gene53274:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTTATTAAGATATCAATT+TGG | - | chr4.1:13220737-13220756 | MS.gene53274:CDS | 15.0% |
| !!! | AAATTAATGATTGGTTAATT+AGG | + | chr4.1:13221412-13221431 | None:intergenic | 15.0% |
| !! | AAATCATTGAACTTAACTAT+GGG | - | chr4.1:13220470-13220489 | MS.gene53274:CDS | 20.0% |
| !! | TACATATTAATACACCTTAT+AGG | - | chr4.1:13221246-13221265 | MS.gene53274:intron | 20.0% |
| !! | TCCTAATTTGACATAATTTA+TGG | - | chr4.1:13220833-13220852 | MS.gene53274:CDS | 20.0% |
| !!! | ATCATAATTAGATAACTTGT+CGG | + | chr4.1:13221010-13221029 | None:intergenic | 20.0% |
| !!! | GCATCATATTTTTCATTTTT+GGG | + | chr4.1:13221548-13221567 | None:intergenic | 20.0% |
| !!! | TGCATCATATTTTTCATTTT+TGG | + | chr4.1:13221549-13221568 | None:intergenic | 20.0% |
| !!! | TTGATTTCTTCTTTTATTTC+AGG | - | chr4.1:13221057-13221076 | MS.gene53274:CDS | 20.0% |
| ! | AATCATTGAACTTAACTATG+GGG | - | chr4.1:13220471-13220490 | MS.gene53274:CDS | 25.0% |
| ! | ACTAACATAATGAAGAATTG+AGG | - | chr4.1:13220698-13220717 | MS.gene53274:CDS | 25.0% |
| ! | ATAGTATTGTAACTGATAAG+TGG | + | chr4.1:13221312-13221331 | None:intergenic | 25.0% |
| ! | ATTAGAAGATGCGTTTAATT+AGG | + | chr4.1:13221112-13221131 | None:intergenic | 25.0% |
| ! | CATCTATTCAAAAACTTTCA+AGG | + | chr4.1:13220913-13220932 | None:intergenic | 25.0% |
| ! | GAAATCATTGAACTTAACTA+TGG | - | chr4.1:13220469-13220488 | MS.gene53274:CDS | 25.0% |
| ! | GCCATAAATTATGTCAAATT+AGG | + | chr4.1:13220837-13220856 | None:intergenic | 25.0% |
| ! | TAATGTCCCTATTTAACATT+GGG | - | chr4.1:13220665-13220684 | MS.gene53274:CDS | 25.0% |
| ! | TGAATGTGATTATACATTGA+TGG | - | chr4.1:13220858-13220877 | MS.gene53274:CDS | 25.0% |
| ! | TTAATGTCCCTATTTAACAT+TGG | - | chr4.1:13220664-13220683 | MS.gene53274:CDS | 25.0% |
| ! | TTATTCCCCAATGTTAAATA+GGG | + | chr4.1:13220674-13220693 | None:intergenic | 25.0% |
| ! | TTTATTCCCCAATGTTAAAT+AGG | + | chr4.1:13220675-13220694 | None:intergenic | 25.0% |
| !! | AAGAATGTTGATTATGTCAA+TGG | + | chr4.1:13221826-13221845 | None:intergenic | 25.0% |
| !! | CTTGAAAGTTTTTGAATAGA+TGG | - | chr4.1:13220911-13220930 | MS.gene53274:CDS | 25.0% |
| !!! | AATGTTGTTGTTTTTGGAAT+GGG | - | chr4.1:13222452-13222471 | MS.gene53274:CDS | 25.0% |
| !!! | ATAAATAGCAAGTTTGCTAT+CGG | + | chr4.1:13222225-13222244 | None:intergenic | 25.0% |
| AAATAGAAACAAACGAAGCA+AGG | + | chr4.1:13222058-13222077 | None:intergenic | 30.0% | |
| AATAGAAACAAACGAAGCAA+GGG | + | chr4.1:13222057-13222076 | None:intergenic | 30.0% | |
| AATGTCCCTATTTAACATTG+GGG | - | chr4.1:13220666-13220685 | MS.gene53274:CDS | 30.0% | |
| AATTGCTAGAAGAAAGAATG+AGG | + | chr4.1:13221589-13221608 | None:intergenic | 30.0% | |
| ACAATGAAGAAGAAGAATGA+AGG | - | chr4.1:13220569-13220588 | MS.gene53274:CDS | 30.0% | |
| ACATTCTTCAAAAAGCCAAA+AGG | - | chr4.1:13221838-13221857 | MS.gene53274:intron | 30.0% | |
| AGGTAAGATTTGTGTATGAT+AGG | + | chr4.1:13221283-13221302 | None:intergenic | 30.0% | |
| CAATGAAGAAGAAGAATGAA+GGG | - | chr4.1:13220570-13220589 | MS.gene53274:CDS | 30.0% | |
| TTTACTCACATCTTCAAAGA+CGG | - | chr4.1:13220973-13220992 | MS.gene53274:CDS | 30.0% | |
| TTTGAACCGTTGTTTAGAAA+TGG | + | chr4.1:13221522-13221541 | None:intergenic | 30.0% | |
| TTTGAGAAGACGAATTAGAA+CGG | + | chr4.1:13221868-13221887 | None:intergenic | 30.0% | |
| ! | AACTTTTTGTCTATCAGTGA+GGG | + | chr4.1:13222015-13222034 | None:intergenic | 30.0% |
| ! | AATGTTGATTATGTCAATGG+TGG | + | chr4.1:13221823-13221842 | None:intergenic | 30.0% |
| ! | ATGAAGGGTTTCTTTCATTT+TGG | - | chr4.1:13220585-13220604 | MS.gene53274:CDS | 30.0% |
| ! | ATGTTGATTATGTCAATGGT+GGG | + | chr4.1:13221822-13221841 | None:intergenic | 30.0% |
| ! | CATTTTATATCTTAGGAGGA+TGG | + | chr4.1:13221435-13221454 | None:intergenic | 30.0% |
| ! | GTTGCATTTTATATCTTAGG+AGG | + | chr4.1:13221439-13221458 | None:intergenic | 30.0% |
| ! | TGCAATGCTTTTATCAGAAA+TGG | + | chr4.1:13221389-13221408 | None:intergenic | 30.0% |
| !! | TAGCAAACTTGCTATTTATC+AGG | - | chr4.1:13222226-13222245 | MS.gene53274:intron | 30.0% |
| !! | TGCTATTTATCAGGTTGATA+AGG | - | chr4.1:13222235-13222254 | MS.gene53274:intron | 30.0% |
| !!! | ACTTTTTGAGACTAAAACGT+TGG | - | chr4.1:13220771-13220790 | MS.gene53274:CDS | 30.0% |
| !!! | GAATGTTGTTGTTTTTGGAA+TGG | - | chr4.1:13222451-13222470 | MS.gene53274:CDS | 30.0% |
| AACCGTTGTTTAGAAATGGT+GGG | + | chr4.1:13221518-13221537 | None:intergenic | 35.0% | |
| AATCAAGTTAGTATGGCTAC+CGG | - | chr4.1:13222164-13222183 | MS.gene53274:intron | 35.0% | |
| AATTCTTCGGTTGTAGATGA+TGG | - | chr4.1:13222350-13222369 | MS.gene53274:intron | 35.0% | |
| AGAATCTTCTGGTGCAAAAA+TGG | + | chr4.1:13221958-13221977 | None:intergenic | 35.0% | |
| ATTGTAACTGATAAGTGGTG+AGG | + | chr4.1:13221307-13221326 | None:intergenic | 35.0% | |
| CAAATCTTCTTTGTCTGTCA+TGG | + | chr4.1:13221349-13221368 | None:intergenic | 35.0% | |
| GAGTAAACAAGAATCACATC+TGG | + | chr4.1:13220960-13220979 | None:intergenic | 35.0% | |
| GATATTGTTGGGGTTTGAAT+TGG | + | chr4.1:13221648-13221667 | None:intergenic | 35.0% | |
| GGAGGATGGAAATTAATGAT+TGG | + | chr4.1:13221421-13221440 | None:intergenic | 35.0% | |
| GGCTGGAAAAACAAATTCTT+CGG | - | chr4.1:13222337-13222356 | MS.gene53274:intron | 35.0% | |
| GGTATAAACAGTACCAGTAA+CGG | + | chr4.1:13222204-13222223 | None:intergenic | 35.0% | |
| TACTAGTGCTTATTCTTGCT+TGG | + | chr4.1:13221491-13221510 | None:intergenic | 35.0% | |
| TTGTAACTGATAAGTGGTGA+GGG | + | chr4.1:13221306-13221325 | None:intergenic | 35.0% | |
| ! | AATTAGAACGGAGAACCTTT+TGG | + | chr4.1:13221856-13221875 | None:intergenic | 35.0% |
| ! | ACTTTTAGCCGTAAAAAAGG+TGG | - | chr4.1:13221188-13221207 | MS.gene53274:intron | 35.0% |
| ! | CAACTTTTTGTCTATCAGTG+AGG | + | chr4.1:13222016-13222035 | None:intergenic | 35.0% |
| ! | CGTACTTTTAGCCGTAAAAA+AGG | - | chr4.1:13221185-13221204 | MS.gene53274:intron | 35.0% |
| ! | CTTTTAGCCGTAAAAAAGGT+GGG | - | chr4.1:13221189-13221208 | MS.gene53274:intron | 35.0% |
| ! | GGGGTTGCATTTTATATCTT+AGG | + | chr4.1:13221442-13221461 | None:intergenic | 35.0% |
| !! | GAATTTGTTTTTCCAGCCTT+AGG | + | chr4.1:13222335-13222354 | None:intergenic | 35.0% |
| !!! | GTTAGAACCCACCTTTTTTA+CGG | + | chr4.1:13221199-13221218 | None:intergenic | 35.0% |
| !!! | TGGCTGAATGTTGTTGTTTT+TGG | - | chr4.1:13222446-13222465 | MS.gene53274:CDS | 35.0% |
| !!! | TGTTGTTTTTGGAATGGGTT+TGG | - | chr4.1:13222457-13222476 | MS.gene53274:CDS | 35.0% |
| AAACTGAGACTCGAATGAGA+AGG | + | chr4.1:13220633-13220652 | None:intergenic | 40.0% | |
| AGGTAGTGTTAGAGCCTATA+AGG | + | chr4.1:13221263-13221282 | None:intergenic | 40.0% | |
| AGGTCTGTATTTCCTTCACA+TGG | + | chr4.1:13220535-13220554 | None:intergenic | 40.0% | |
| CAGTGGCAATCAAGTTAGTA+TGG | - | chr4.1:13222157-13222176 | MS.gene53274:intron | 40.0% | |
| CATTCTCCAAACTCAAAGCA+GGG | - | chr4.1:13221979-13221998 | MS.gene53274:intron | 40.0% | |
| CATTGAACTTAACTATGGGG+CGG | - | chr4.1:13220474-13220493 | MS.gene53274:CDS | 40.0% | |
| CTCCCACCATTTCTAAACAA+CGG | - | chr4.1:13221513-13221532 | MS.gene53274:intron | 40.0% | |
| CTTCTTTGTCTGTCATGGAA+AGG | + | chr4.1:13221344-13221363 | None:intergenic | 40.0% | |
| GAACCGTTGTTTAGAAATGG+TGG | + | chr4.1:13221519-13221538 | None:intergenic | 40.0% | |
| GGAAATACAGACCTGAAACA+AGG | - | chr4.1:13220541-13220560 | MS.gene53274:CDS | 40.0% | |
| GGTTTGAATTGGTGAGAGTT+GGG | + | chr4.1:13221637-13221656 | None:intergenic | 40.0% | |
| GTGAGGGTGATGATATTGTT+GGG | + | chr4.1:13221659-13221678 | None:intergenic | 40.0% | |
| GTTCTAACACTTCATGCAGA+TGG | - | chr4.1:13221211-13221230 | MS.gene53274:intron | 40.0% | |
| TGAGGGTGATGATATTGTTG+GGG | + | chr4.1:13221658-13221677 | None:intergenic | 40.0% | |
| TGCTAGAAGAAAGAATGAGG+TGG | + | chr4.1:13221586-13221605 | None:intergenic | 40.0% | |
| TGCTAGCTCATCAAGATACA+AGG | + | chr4.1:13221163-13221182 | None:intergenic | 40.0% | |
| TTGGAGAATGCAGAATCTTC+TGG | + | chr4.1:13221969-13221988 | None:intergenic | 40.0% | |
| ! | CATTGTGAGTTCCTTGTTTC+AGG | + | chr4.1:13220555-13220574 | None:intergenic | 40.0% |
| !! | ATTCTTGCTTGGTGATGTTG+TGG | + | chr4.1:13221480-13221499 | None:intergenic | 40.0% |
| !! | GATGCTGTTGTTGATGGTAA+GGG | + | chr4.1:13221684-13221703 | None:intergenic | 40.0% |
| !! | TGATGCTGTTGTTGATGGTA+AGG | + | chr4.1:13221685-13221704 | None:intergenic | 40.0% |
| !! | TGTTTGAACAGGGTTGGTTA+AGG | + | chr4.1:13222093-13222112 | None:intergenic | 40.0% |
| !!! | GTTTTTGGAATGGGTTTGGT+TGG | - | chr4.1:13222461-13222480 | MS.gene53274:CDS | 40.0% |
| AACCCTGTTCAAACACAAGC+CGG | - | chr4.1:13222098-13222117 | MS.gene53274:intron | 45.0% | |
| AACGGTAGCATTAACTGCTC+CGG | + | chr4.1:13222186-13222205 | None:intergenic | 45.0% | |
| ACTGTGCTTAATCCTGGTGA+TGG | + | chr4.1:13221738-13221757 | None:intergenic | 45.0% | |
| AGAGTTGGGCTAATGTGGTT+GGG | + | chr4.1:13221623-13221642 | None:intergenic | 45.0% | |
| ATGAGAAGGACCAAATGACG+TGG | + | chr4.1:13220619-13220638 | None:intergenic | 45.0% | |
| CAGCTAGTATCATCACCATC+AGG | - | chr4.1:13221918-13221937 | MS.gene53274:intron | 45.0% | |
| CTGAACGTCACTACTTACAG+TGG | - | chr4.1:13222140-13222159 | MS.gene53274:intron | 45.0% | |
| CTTGTTCCAACAACACCAAC+TGG | - | chr4.1:13221762-13221781 | MS.gene53274:intron | 45.0% | |
| CTTTGTCTGTCATGGAAAGG+TGG | + | chr4.1:13221341-13221360 | None:intergenic | 45.0% | |
| GCAGTTAATGCTACCGTTAC+TGG | - | chr4.1:13222188-13222207 | MS.gene53274:intron | 45.0% | |
| GCATTCTCCAAACTCAAAGC+AGG | - | chr4.1:13221978-13221997 | MS.gene53274:intron | 45.0% | |
| GCCTCTTGATCTTGTTCTTC+CGG | - | chr4.1:13222265-13222284 | MS.gene53274:intron | 45.0% | |
| GGGTTTGAATTGGTGAGAGT+TGG | + | chr4.1:13221638-13221657 | None:intergenic | 45.0% | |
| GGTGAGGGTGATGATATTGT+TGG | + | chr4.1:13221660-13221679 | None:intergenic | 45.0% | |
| GGTGATGTTGTGGATGAAGA+GGG | + | chr4.1:13221470-13221489 | None:intergenic | 45.0% | |
| GTATCATCACCATCAGGTTC+AGG | - | chr4.1:13221924-13221943 | MS.gene53274:intron | 45.0% | |
| GTGATGTTGTGGATGAAGAG+GGG | + | chr4.1:13221469-13221488 | None:intergenic | 45.0% | |
| TAACTGATAAGTGGTGAGGG+AGG | + | chr4.1:13221303-13221322 | None:intergenic | 45.0% | |
| TCACGAATCTTGCAAGTCAG+AGG | + | chr4.1:13220506-13220525 | None:intergenic | 45.0% | |
| TGATCTTGTTCTTCCGGCTA+AGG | - | chr4.1:13222271-13222290 | MS.gene53274:intron | 45.0% | |
| TTAGGCAACAAACCCTTAGC+CGG | + | chr4.1:13222317-13222336 | None:intergenic | 45.0% | |
| TTGAAGCCTAGCATCATCAC+CGG | + | chr4.1:13222120-13222139 | None:intergenic | 45.0% | |
| ! | ATACTAGCTGCGAGTTGAGT+TGG | + | chr4.1:13221908-13221927 | None:intergenic | 45.0% |
| ! | GTTGCTGTTGTTGCTGGTAA+TGG | + | chr4.1:13221711-13221730 | None:intergenic | 45.0% |
| ! | TCGGTTGTAGATGATGGTAG+CGG | - | chr4.1:13222356-13222375 | MS.gene53274:intron | 45.0% |
| ! | TGGTGATGTTGTGGATGAAG+AGG | + | chr4.1:13221471-13221490 | None:intergenic | 45.0% |
| ! | TTTGGAATGGGTTTGGTTGG+TGG | - | chr4.1:13222464-13222483 | MS.gene53274:CDS | 45.0% |
| !! | CAGTGATGATGGTGATGGAA+AGG | - | chr4.1:13222385-13222404 | MS.gene53274:intron | 45.0% |
| !! | GTTGGTGTTGTTGGAACAAG+CGG | + | chr4.1:13221762-13221781 | None:intergenic | 45.0% |
| !!! | ATGTCAATGGTGGGTCCTTT+TGG | + | chr4.1:13221813-13221832 | None:intergenic | 45.0% |
| !!! | CCTTTTGGGATTGCTTTGGT+GGG | + | chr4.1:13221798-13221817 | None:intergenic | 45.0% |
| !!! | CTTTTGGGATTGCTTTGGTG+GGG | + | chr4.1:13221797-13221816 | None:intergenic | 45.0% |
| !!! | TCCTTTTGGGATTGCTTTGG+TGG | + | chr4.1:13221799-13221818 | None:intergenic | 45.0% |
| !!! | TGTCAATGGTGGGTCCTTTT+GGG | + | chr4.1:13221812-13221831 | None:intergenic | 45.0% |
| !!! | TTTTGGGATTGCTTTGGTGG+GGG | + | chr4.1:13221796-13221815 | None:intergenic | 45.0% |
| AGCGGAACTGTGCTTAATCC+TGG | + | chr4.1:13221744-13221763 | None:intergenic | 50.0% | |
| CATCACCATCAGGTTCAGGA+GGG | - | chr4.1:13221928-13221947 | MS.gene53274:intron | 50.0% | |
| CCCACCAAAGCAATCCCAAA+AGG | - | chr4.1:13221795-13221814 | MS.gene53274:intron | 50.0% | |
| GAGAGTTGGGCTAATGTGGT+TGG | + | chr4.1:13221624-13221643 | None:intergenic | 50.0% | |
| GAGTTGGGCTAATGTGGTTG+GGG | + | chr4.1:13221622-13221641 | None:intergenic | 50.0% | |
| GCCGGAAGAACAAGATCAAG+AGG | + | chr4.1:13222269-13222288 | None:intergenic | 50.0% | |
| GGACCAAATGACGTGGTACA+TGG | + | chr4.1:13220612-13220631 | None:intergenic | 50.0% | |
| GGCTTGTGTTTGAACAGGGT+TGG | + | chr4.1:13222099-13222118 | None:intergenic | 50.0% | |
| GGTCCATGTACCACGTCATT+TGG | - | chr4.1:13220606-13220625 | MS.gene53274:CDS | 50.0% | |
| GTCTATCAGTGAGGGAGTTG+AGG | + | chr4.1:13222007-13222026 | None:intergenic | 50.0% | |
| GTTAACCCTCCTGAACCTGA+TGG | + | chr4.1:13221936-13221955 | None:intergenic | 50.0% | |
| TCATCACCATCAGGTTCAGG+AGG | - | chr4.1:13221927-13221946 | MS.gene53274:intron | 50.0% | |
| TCGTGAGCACTACCATGTGA+AGG | - | chr4.1:13220520-13220539 | MS.gene53274:CDS | 50.0% | |
| TGGTGAGAGTTGGGCTAATG+TGG | + | chr4.1:13221628-13221647 | None:intergenic | 50.0% | |
| TGTGGATGAAGAGGGGAGAA+GGG | + | chr4.1:13221462-13221481 | None:intergenic | 50.0% | |
| TTGTGGATGAAGAGGGGAGA+AGG | + | chr4.1:13221463-13221482 | None:intergenic | 50.0% | |
| ! | GAGGAACCCTGCTTTGAGTT+TGG | + | chr4.1:13221988-13222007 | None:intergenic | 50.0% |
| ! | GGTGGTGATGCTGTTGTTGA+TGG | + | chr4.1:13221690-13221709 | None:intergenic | 50.0% |
| !! | AAGGGTTTGTTGCCTAAGGC+TGG | - | chr4.1:13222320-13222339 | MS.gene53274:intron | 50.0% |
| !! | CACCGGCTTGTGTTTGAACA+GGG | + | chr4.1:13222103-13222122 | None:intergenic | 50.0% |
| !! | GCTGTTGTTGATGGTAAGGG+TGG | + | chr4.1:13221681-13221700 | None:intergenic | 50.0% |
| !! | GCTGTTGTTGCTGGTAATGG+TGG | + | chr4.1:13221708-13221727 | None:intergenic | 50.0% |
| !! | GGCTAAGGGTTTGTTGCCTA+AGG | - | chr4.1:13222316-13222335 | MS.gene53274:intron | 50.0% |
| !! | GTTGATGGTAAGGGTGGTGA+GGG | + | chr4.1:13221675-13221694 | None:intergenic | 50.0% |
| !! | TCACCGGCTTGTGTTTGAAC+AGG | + | chr4.1:13222104-13222123 | None:intergenic | 50.0% |
| !! | TGTTGATGGTAAGGGTGGTG+AGG | + | chr4.1:13221676-13221695 | None:intergenic | 50.0% |
| !!! | GGGTCCTTTTGGGATTGCTT+TGG | + | chr4.1:13221802-13221821 | None:intergenic | 50.0% |
| AACAAACCCTTAGCCGGTGC+AGG | + | chr4.1:13222311-13222330 | None:intergenic | 55.0% | |
| ACAGCAACAGCACCATCACC+AGG | - | chr4.1:13221723-13221742 | MS.gene53274:intron | 55.0% | |
| CCTGCAGCAGAAACATCAGC+AGG | + | chr4.1:13222416-13222435 | None:intergenic | 55.0% | |
| CTGCTGCAGGATCTGTGCAT+TGG | - | chr4.1:13222426-13222445 | MS.gene53274:CDS | 55.0% | |
| GTGGATGAAGAGGGGAGAAG+GGG | + | chr4.1:13221461-13221480 | None:intergenic | 55.0% | |
| ! | CACAAGCCGGTGATGATGCT+AGG | - | chr4.1:13222111-13222130 | MS.gene53274:intron | 55.0% |
| ! | CCTGCTGATGTTTCTGCTGC+AGG | - | chr4.1:13222413-13222432 | MS.gene53274:CDS | 55.0% |
| !! | GCTGGCAGTGATGATGGTGA+TGG | - | chr4.1:13222380-13222399 | MS.gene53274:intron | 55.0% |
| !! | GGTGCACCAGTTGGTGTTGT+TGG | + | chr4.1:13221771-13221790 | None:intergenic | 55.0% |
| !! | GGTGCTGTTGCTGTTGTTGC+TGG | + | chr4.1:13221717-13221736 | None:intergenic | 55.0% |
| !! | GTAGATGATGGTAGCGGTGC+TGG | - | chr4.1:13222362-13222381 | MS.gene53274:intron | 55.0% |
| GCCAAAGCTGGAGCCTTAGC+CGG | + | chr4.1:13222287-13222306 | None:intergenic | 60.0% | |
| GGGATTGCTTTGGTGGGGGA+TGG | + | chr4.1:13221792-13221811 | None:intergenic | 60.0% | |
| TCCGGCTAAGGCTCCAGCTT+TGG | - | chr4.1:13222283-13222302 | MS.gene53274:intron | 60.0% | |
| ! | TCCAGCTTTGGCTCCTGCAC+CGG | - | chr4.1:13222295-13222314 | MS.gene53274:intron | 60.0% |
| !! | AGCGGTGCTGGCAGTGATGA+TGG | - | chr4.1:13222374-13222393 | MS.gene53274:intron | 60.0% |
| !! | TTGGCTCCTGCACCGGCTAA+GGG | - | chr4.1:13222302-13222321 | MS.gene53274:intron | 60.0% |
| !! | TTTGGCTCCTGCACCGGCTA+AGG | - | chr4.1:13222301-13222320 | MS.gene53274:intron | 60.0% |
| GTGGGGGATGGTGCACCAGT+TGG | + | chr4.1:13221780-13221799 | None:intergenic | 65.0% | |
| GCCGGTGCAGGAGCCAAAGC+TGG | + | chr4.1:13222299-13222318 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 13220427 | 13222499 | 13220427 | ID=MS.gene53274 |
| chr4.1 | mRNA | 13220427 | 13222499 | 13220427 | ID=MS.gene53274.t1;Parent=MS.gene53274 |
| chr4.1 | exon | 13222405 | 13222499 | 13222405 | ID=MS.gene53274.t1.exon1;Parent=MS.gene53274.t1 |
| chr4.1 | CDS | 13222405 | 13222499 | 13222405 | ID=cds.MS.gene53274.t1;Parent=MS.gene53274.t1 |
| chr4.1 | exon | 13220427 | 13221174 | 13220427 | ID=MS.gene53274.t1.exon2;Parent=MS.gene53274.t1 |
| chr4.1 | CDS | 13220427 | 13221174 | 13220427 | ID=cds.MS.gene53274.t1;Parent=MS.gene53274.t1 |
| Gene Sequence |
| Protein sequence |
