Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53469.t1 | GAU25724.1 | 71.7 | 53 | 15 | 0 | 5 | 57 | 66 | 118 | 9.20E-12 | 79.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53469.t1 | A0A2Z6LZX5 | 71.7 | 53 | 15 | 0 | 5 | 57 | 66 | 118 | 6.7e-12 | 79.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053298 | MS.gene53469 | 0.804978 | 1.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53469.t1 | MTR_5g069640 | 67.925 | 53 | 17 | 0 | 5 | 57 | 66 | 118 | 1.80e-20 | 80.9 |
| MS.gene53469.t1 | MTR_8g093450 | 92.308 | 39 | 3 | 0 | 72 | 110 | 57 | 95 | 9.07e-20 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53469.t1 | AT2G29590 | 58.491 | 53 | 22 | 0 | 5 | 57 | 63 | 115 | 2.23e-15 | 67.8 |
Find 26 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCCTCCTCTTTGTTAAAC+TGG | 0.287597 | 5.3:+23086132 | None:intergenic |
| TTTCACTTTCAATGTACTCT+TGG | 0.335237 | 5.3:-23087073 | None:intergenic |
| AAGAAAAGATGAAAAGTGTA+TGG | 0.395163 | 5.3:-23086097 | MS.gene53469:CDS |
| GTACTGTACCTGATACAATA+TGG | 0.402588 | 5.3:+23086265 | None:intergenic |
| GTGAAAGATAAGAAAGATCT+TGG | 0.408538 | 5.3:-23086182 | MS.gene53469:CDS |
| GAGGAGAACCATATTGTATC+AGG | 0.416982 | 5.3:-23086273 | MS.gene53469:intron |
| CATATCAACATAAACATTCA+TGG | 0.435825 | 5.3:+23086462 | None:intergenic |
| GTTTAACAAAGAGGAGGGAT+TGG | 0.488431 | 5.3:-23086129 | MS.gene53469:CDS |
| AACACTGGAAAGTTGGCTAA+TGG | 0.488900 | 5.3:-23086544 | MS.gene53469:CDS |
| CAATATGGTTCTCCTCTTCA+CGG | 0.490112 | 5.3:+23086280 | None:intergenic |
| GGGGCTGTAATCCATGGAAA+TGG | 0.501769 | 5.3:-23086490 | MS.gene53469:CDS |
| ACAGGACAACACTGGAAAGT+TGG | 0.504393 | 5.3:-23086551 | MS.gene53469:intron |
| TCATGGGGAGACCATTTCCA+TGG | 0.527491 | 5.3:+23086479 | None:intergenic |
| GAATATCTTGAGGAATATGT+TGG | 0.532265 | 5.3:-23086305 | MS.gene53469:CDS |
| AGAAAAGATGAAAAGTGTAT+GGG | 0.540681 | 5.3:-23086096 | MS.gene53469:CDS |
| GTTGGAGGGGCTGTAATCCA+TGG | 0.544476 | 5.3:-23086496 | MS.gene53469:CDS |
| ATATCAACATAAACATTCAT+GGG | 0.545871 | 5.3:+23086463 | None:intergenic |
| TACCAGTTTAACAAAGAGGA+GGG | 0.568079 | 5.3:-23086134 | MS.gene53469:CDS |
| GTATTACCAGTTTAACAAAG+AGG | 0.598288 | 5.3:-23086138 | MS.gene53469:CDS |
| CCTCTCTTACAGGACAACAC+TGG | 0.607683 | 5.3:-23086559 | MS.gene53469:intron |
| TCTTGGAATCATGAAAGTCA+AGG | 0.609272 | 5.3:-23086165 | MS.gene53469:CDS |
| TTACCAGTTTAACAAAGAGG+AGG | 0.617647 | 5.3:-23086135 | MS.gene53469:CDS |
| TCACGTGCAGGAATATCTTG+AGG | 0.635000 | 5.3:-23086315 | MS.gene53469:intron |
| AAGATGAAAAGTGTATGGGA+AGG | 0.640402 | 5.3:-23086092 | MS.gene53469:CDS |
| AATATGTTGGAACCGTGAAG+AGG | 0.650450 | 5.3:-23086292 | MS.gene53469:CDS |
| TATCAACATAAACATTCATG+GGG | 0.688765 | 5.3:+23086464 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATTACTTGAAATAAAA+AGG | - | chr5.3:23086216-23086235 | MS.gene53469:intron | 10.0% |
| !! | TATTTATACAAATATCAAAT+AGG | + | chr5.3:23086395-23086414 | None:intergenic | 10.0% |
| !! | AATAATCCTTTATTAGTTAA+GGG | - | chr5.3:23086460-23086479 | MS.gene53469:CDS | 15.0% |
| !! | AGATTAAATTTAAGAAAAAC+TGG | - | chr5.3:23086517-23086536 | MS.gene53469:CDS | 15.0% |
| !! | TAATAATCCTTTATTAGTTA+AGG | - | chr5.3:23086459-23086478 | MS.gene53469:CDS | 15.0% |
| !! | TTATTCTATATAGAATTGAA+TGG | - | chr5.3:23086546-23086565 | MS.gene53469:CDS | 15.0% |
| !!! | ATAAAGGATTATTATTTCTA+TGG | + | chr5.3:23086453-23086472 | None:intergenic | 15.0% |
| !! | ATATAGAATTGAATGGTAAA+AGG | - | chr5.3:23086553-23086572 | MS.gene53469:intron | 20.0% |
| !! | ATATCAACATAAACATTCAT+GGG | + | chr5.3:23086687-23086706 | None:intergenic | 20.0% |
| !! | TCATGTTAACATAAAAGATT+AGG | - | chr5.3:23086750-23086769 | MS.gene53469:intron | 20.0% |
| ! | CATATCAACATAAACATTCA+TGG | + | chr5.3:23086688-23086707 | None:intergenic | 25.0% |
| ! | CTGAAAAGAATCTTCAATTT+AGG | - | chr5.3:23086908-23086927 | MS.gene53469:intron | 25.0% |
| ! | TATCAACATAAACATTCATG+GGG | + | chr5.3:23086686-23086705 | None:intergenic | 25.0% |
| !! | AAATTTTATATATCACGTGC+AGG | - | chr5.3:23086820-23086839 | MS.gene53469:intron | 25.0% |
| !! | AAGAAAAGATGAAAAGTGTA+TGG | - | chr5.3:23087050-23087069 | MS.gene53469:intron | 25.0% |
| !! | AGAAAAGATGAAAAGTGTAT+GGG | - | chr5.3:23087051-23087070 | MS.gene53469:intron | 25.0% |
| !! | GATTTTTGTTGAATGTTGTT+TGG | - | chr5.3:23086249-23086268 | MS.gene53469:intron | 25.0% |
| !!! | ACACAACTATTGATTTTTCT+AGG | - | chr5.3:23086328-23086347 | MS.gene53469:intron | 25.0% |
| !!! | TCGTAGTTATTTTATTGAGA+AGG | + | chr5.3:23086155-23086174 | None:intergenic | 25.0% |
| ATCTTTCTTATCTTTCACCT+TGG | + | chr5.3:23086963-23086982 | None:intergenic | 30.0% | |
| GAATATCTTGAGGAATATGT+TGG | - | chr5.3:23086842-23086861 | MS.gene53469:intron | 30.0% | |
| GTATTACCAGTTTAACAAAG+AGG | - | chr5.3:23087009-23087028 | MS.gene53469:intron | 30.0% | |
| GTGAAAGATAAGAAAGATCT+TGG | - | chr5.3:23086965-23086984 | MS.gene53469:intron | 30.0% | |
| TCTTTCTTATCTTTCACCTT+GGG | + | chr5.3:23086962-23086981 | None:intergenic | 30.0% | |
| TGTGTTAATGTGCTATCTTT+AGG | - | chr5.3:23086782-23086801 | MS.gene53469:intron | 30.0% | |
| ! | ATTGAGAAGGTTTTCCTTTA+CGG | + | chr5.3:23086142-23086161 | None:intergenic | 30.0% |
| AGAGGTCATACAATCTTACT+TGG | - | chr5.3:23086487-23086506 | MS.gene53469:CDS | 35.0% | |
| CTCACACCCTTAACTAATAA+AGG | + | chr5.3:23086469-23086488 | None:intergenic | 35.0% | |
| GTACTGTACCTGATACAATA+TGG | + | chr5.3:23086885-23086904 | None:intergenic | 35.0% | |
| TACCAGTTTAACAAAGAGGA+GGG | - | chr5.3:23087013-23087032 | MS.gene53469:intron | 35.0% | |
| TCTTGGAATCATGAAAGTCA+AGG | - | chr5.3:23086982-23087001 | MS.gene53469:intron | 35.0% | |
| TCTTGGATTTGTGTATCTAG+AGG | - | chr5.3:23086091-23086110 | MS.gene53469:CDS | 35.0% | |
| TGTTTGGATTGTTTGCGATT+TGG | - | chr5.3:23086265-23086284 | MS.gene53469:intron | 35.0% | |
| TTACCAGTTTAACAAAGAGG+AGG | - | chr5.3:23087012-23087031 | MS.gene53469:intron | 35.0% | |
| ! | AAGATGAAAAGTGTATGGGA+AGG | - | chr5.3:23087055-23087074 | MS.gene53469:intron | 35.0% |
| ! | TCAACTTTTGTGAGTGAAGT+TGG | - | chr5.3:23086633-23086652 | MS.gene53469:intron | 35.0% |
| ! | TTATTAGTTAAGGGTGTGAG+AGG | - | chr5.3:23086469-23086488 | MS.gene53469:CDS | 35.0% |
| AACACTGGAAAGTTGGCTAA+TGG | - | chr5.3:23086603-23086622 | MS.gene53469:intron | 40.0% | |
| AAGCACGAACCAGAGAAATT+GGG | - | chr5.3:23086174-23086193 | MS.gene53469:CDS | 40.0% | |
| AATATGTTGGAACCGTGAAG+AGG | - | chr5.3:23086855-23086874 | MS.gene53469:intron | 40.0% | |
| ACGACAAAACCCAATTTCTC+TGG | + | chr5.3:23086186-23086205 | None:intergenic | 40.0% | |
| ATCCCTCCTCTTTGTTAAAC+TGG | + | chr5.3:23087018-23087037 | None:intergenic | 40.0% | |
| CAATATGGTTCTCCTCTTCA+CGG | + | chr5.3:23086870-23086889 | None:intergenic | 40.0% | |
| CTAGAGGAATACATATCTCG+TGG | - | chr5.3:23086107-23086126 | MS.gene53469:CDS | 40.0% | |
| GAGGAGAACCATATTGTATC+AGG | - | chr5.3:23086874-23086893 | MS.gene53469:intron | 40.0% | |
| GTCATGACTTGATATACCCA+AGG | - | chr5.3:23086943-23086962 | MS.gene53469:intron | 40.0% | |
| GTTTAACAAAGAGGAGGGAT+TGG | - | chr5.3:23087018-23087037 | MS.gene53469:intron | 40.0% | |
| ! | ACTTTTGTGAGTGAAGTTGG+AGG | - | chr5.3:23086636-23086655 | MS.gene53469:intron | 40.0% |
| ! | CTTTTGTGAGTGAAGTTGGA+GGG | - | chr5.3:23086637-23086656 | MS.gene53469:intron | 40.0% |
| ! | GTTACTTTCACCTCTCTTAC+AGG | - | chr5.3:23086578-23086597 | MS.gene53469:intron | 40.0% |
| ! | TTTTGTGAGTGAAGTTGGAG+GGG | - | chr5.3:23086638-23086657 | MS.gene53469:intron | 40.0% |
| !! | AGAAATTGGGTTTTGTCGTC+GGG | - | chr5.3:23086187-23086206 | MS.gene53469:intron | 40.0% |
| !! | GAGAAATTGGGTTTTGTCGT+CGG | - | chr5.3:23086186-23086205 | MS.gene53469:intron | 40.0% |
| ACAGGACAACACTGGAAAGT+TGG | - | chr5.3:23086596-23086615 | MS.gene53469:intron | 45.0% | |
| GAAGCACGAACCAGAGAAAT+TGG | - | chr5.3:23086173-23086192 | MS.gene53469:CDS | 45.0% | |
| TCACGTGCAGGAATATCTTG+AGG | - | chr5.3:23086832-23086851 | MS.gene53469:intron | 45.0% | |
| CCAGTGTTGTCCTGTAAGAG+AGG | + | chr5.3:23086591-23086610 | None:intergenic | 50.0% | |
| CCTCTCTTACAGGACAACAC+TGG | - | chr5.3:23086588-23086607 | MS.gene53469:intron | 50.0% | |
| CGTGGATTTGCGAACCGTAA+AGG | - | chr5.3:23086125-23086144 | MS.gene53469:CDS | 50.0% | |
| GGGGCTGTAATCCATGGAAA+TGG | - | chr5.3:23086657-23086676 | MS.gene53469:intron | 50.0% | |
| TCATGGGGAGACCATTTCCA+TGG | + | chr5.3:23086671-23086690 | None:intergenic | 50.0% | |
| GTTGGAGGGGCTGTAATCCA+TGG | - | chr5.3:23086651-23086670 | MS.gene53469:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 23086085 | 23087084 | 23086085 | ID=MS.gene53469 |
| chr5.3 | mRNA | 23086085 | 23087084 | 23086085 | ID=MS.gene53469.t1;Parent=MS.gene53469 |
| chr5.3 | exon | 23087067 | 23087084 | 23087067 | ID=MS.gene53469.t1.exon1;Parent=MS.gene53469.t1 |
| chr5.3 | CDS | 23087067 | 23087084 | 23087067 | ID=cds.MS.gene53469.t1;Parent=MS.gene53469.t1 |
| chr5.3 | exon | 23086429 | 23086569 | 23086429 | ID=MS.gene53469.t1.exon2;Parent=MS.gene53469.t1 |
| chr5.3 | CDS | 23086429 | 23086569 | 23086429 | ID=cds.MS.gene53469.t1;Parent=MS.gene53469.t1 |
| chr5.3 | exon | 23086274 | 23086327 | 23086274 | ID=MS.gene53469.t1.exon3;Parent=MS.gene53469.t1 |
| chr5.3 | CDS | 23086274 | 23086327 | 23086274 | ID=cds.MS.gene53469.t1;Parent=MS.gene53469.t1 |
| chr5.3 | exon | 23086085 | 23086204 | 23086085 | ID=MS.gene53469.t1.exon4;Parent=MS.gene53469.t1 |
| chr5.3 | CDS | 23086085 | 23086204 | 23086085 | ID=cds.MS.gene53469.t1;Parent=MS.gene53469.t1 |
| Gene Sequence |
| Protein sequence |
