Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53563.t1 | XP_003590183.2 | 92.8 | 982 | 28 | 1 | 20 | 958 | 146 | 1127 | 0.00E+00 | 1812 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53563.t1 | Q84LK0 | 67.5 | 925 | 273 | 4 | 20 | 943 | 144 | 1041 | 0.0e+00 | 1236.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53563.t1 | G7I6Q0 | 92.8 | 982 | 28 | 1 | 20 | 958 | 146 | 1127 | 0.0e+00 | 1812.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene53563 | 0.807783 | 4.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53563.t1 | MTR_1g045670 | 92.770 | 982 | 28 | 1 | 20 | 958 | 146 | 1127 | 0.0 | 1871 |
| MS.gene53563.t1 | MTR_1g045660 | 90.123 | 81 | 6 | 1 | 953 | 1033 | 50 | 128 | 3.39e-39 | 143 |
| MS.gene53563.t1 | MTR_7g109470 | 38.021 | 192 | 111 | 4 | 634 | 818 | 1023 | 1213 | 2.32e-25 | 114 |
| MS.gene53563.t1 | MTR_5g068600 | 33.333 | 195 | 120 | 4 | 638 | 827 | 330 | 519 | 7.39e-21 | 99.0 |
| MS.gene53563.t1 | MTR_5g068600 | 33.333 | 195 | 120 | 4 | 638 | 827 | 430 | 619 | 8.37e-21 | 99.0 |
| MS.gene53563.t1 | MTR_6g086270 | 32.474 | 194 | 111 | 4 | 639 | 817 | 897 | 1085 | 4.42e-19 | 94.0 |
| MS.gene53563.t1 | MTR_4g111945 | 31.771 | 192 | 119 | 4 | 640 | 819 | 664 | 855 | 3.84e-18 | 90.5 |
| MS.gene53563.t1 | MTR_4g088920 | 27.586 | 203 | 135 | 3 | 617 | 818 | 414 | 605 | 7.98e-17 | 86.3 |
| MS.gene53563.t1 | MTR_1g100683 | 30.279 | 251 | 149 | 7 | 584 | 817 | 747 | 988 | 9.50e-16 | 83.2 |
| MS.gene53563.t1 | MTR_1g100683 | 30.279 | 251 | 149 | 7 | 584 | 817 | 747 | 988 | 9.50e-16 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53563.t1 | AT3G24320 | 67.459 | 925 | 273 | 4 | 20 | 943 | 144 | 1041 | 0.0 | 1276 |
| MS.gene53563.t1 | AT3G24320 | 65.249 | 823 | 258 | 4 | 122 | 943 | 1 | 796 | 0.0 | 1079 |
| MS.gene53563.t1 | AT1G04555 | 71.429 | 77 | 20 | 1 | 957 | 1033 | 52 | 126 | 1.65e-27 | 109 |
| MS.gene53563.t1 | AT4G02070 | 36.634 | 202 | 117 | 6 | 622 | 817 | 1064 | 1260 | 1.02e-23 | 108 |
| MS.gene53563.t1 | AT4G02070 | 36.634 | 202 | 117 | 6 | 622 | 817 | 1067 | 1263 | 1.07e-23 | 108 |
| MS.gene53563.t1 | AT5G54090 | 34.649 | 228 | 132 | 6 | 617 | 839 | 424 | 639 | 8.07e-22 | 102 |
| MS.gene53563.t1 | AT3G24495 | 28.919 | 370 | 204 | 15 | 516 | 844 | 711 | 1062 | 5.49e-21 | 100 |
| MS.gene53563.t1 | AT4G25540 | 32.381 | 210 | 124 | 6 | 640 | 838 | 814 | 1016 | 1.03e-16 | 86.3 |
| MS.gene53563.t1 | AT3G18524 | 30.208 | 192 | 120 | 4 | 640 | 819 | 663 | 852 | 1.77e-16 | 85.5 |
| MS.gene53563.t1 | AT4G17380 | 27.727 | 220 | 143 | 4 | 637 | 855 | 547 | 751 | 6.77e-15 | 80.1 |
| MS.gene53563.t1 | AT1G65070 | 30.939 | 181 | 122 | 2 | 638 | 817 | 397 | 575 | 4.11e-14 | 77.4 |
| MS.gene53563.t1 | AT1G65070 | 30.939 | 181 | 122 | 2 | 638 | 817 | 397 | 575 | 4.24e-14 | 77.4 |
| MS.gene53563.t1 | AT1G65070 | 30.939 | 181 | 122 | 2 | 638 | 817 | 397 | 575 | 4.98e-14 | 77.0 |
| MS.gene53563.t1 | AT1G65070 | 30.939 | 181 | 122 | 2 | 638 | 817 | 397 | 575 | 5.02e-14 | 77.4 |
| MS.gene53563.t1 | AT4G17380 | 32.258 | 124 | 84 | 0 | 637 | 760 | 547 | 670 | 7.36e-14 | 76.6 |
Find 255 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTATGTATATGGACTTAT+TGG | 0.101408 | 1.2:-28562849 | MS.gene53563:CDS |
| TTTGAAATAACCTTGAATTT+TGG | 0.143407 | 1.2:+28560084 | None:intergenic |
| AAGATAAAGTGTTTCTTAAT+TGG | 0.179571 | 1.2:-28549444 | MS.gene53563:CDS |
| CAACCCTGTTTCTGATCTTT+TGG | 0.186678 | 1.2:-28561304 | MS.gene53563:CDS |
| GTAGATGTTCCAAGAGTTAA+AGG | 0.212866 | 1.2:+28561088 | None:intergenic |
| CTTTGGTTCTCAGGTATGTT+AGG | 0.214976 | 1.2:-28560144 | MS.gene53563:intron |
| TAACTGTGTCTTCGGGAAAT+AGG | 0.228381 | 1.2:-28561117 | MS.gene53563:CDS |
| TATATACTACCTCAAGCTTC+TGG | 0.228940 | 1.2:+28552731 | None:intergenic |
| TGATCACAAAAGTTCATTTC+AGG | 0.235726 | 1.2:-28551628 | MS.gene53563:intron |
| GATAAAGATCTTCAGCTCTT+TGG | 0.239855 | 1.2:+28549721 | None:intergenic |
| CTTGGAACATCTACTCAAAT+TGG | 0.241948 | 1.2:-28561079 | MS.gene53563:intron |
| CTGCGGTTGATTTGTCTAAA+AGG | 0.245303 | 1.2:-28536562 | MS.gene53563:CDS |
| CGTCAGCTATATTGCTTAAC+AGG | 0.258185 | 1.2:+28548553 | None:intergenic |
| TAGCCATATCTGAACCAATT+TGG | 0.260142 | 1.2:-28534127 | MS.gene53563:CDS |
| AGATATAAAACGACGTTTCT+TGG | 0.262650 | 1.2:+28563064 | None:intergenic |
| TAGCATGATAGAGTCAAAAT+AGG | 0.263250 | 1.2:+28551677 | None:intergenic |
| ACTTGGGATATGTGGACTTA+TGG | 0.268862 | 1.2:-28551724 | MS.gene53563:CDS |
| TTCTTGGTTCGAGCTTGAGC+TGG | 0.277875 | 1.2:+28563080 | None:intergenic |
| CGTCCCACGTGGGTGGCTAC+TGG | 0.278644 | 1.2:-28558790 | MS.gene53563:CDS |
| TTGCCTTGACATTTGCTTCA+TGG | 0.279311 | 1.2:+28553883 | None:intergenic |
| CTTGATAGAAGTGGCTGTTT+GGG | 0.282969 | 1.2:-28549933 | MS.gene53563:CDS |
| TTCTTTGGTTTCAGAATCTT+AGG | 0.288160 | 1.2:-28563378 | MS.gene53563:intron |
| CCAGGAAACCCCTATGTATA+TGG | 0.292216 | 1.2:-28562858 | MS.gene53563:CDS |
| CAAGGCAAACACTAGAGTTT+TGG | 0.296309 | 1.2:-28553868 | MS.gene53563:CDS |
| TGATTTGTCTAAAAGGTTAA+AGG | 0.297744 | 1.2:-28536555 | MS.gene53563:CDS |
| ATCAATGGTAATAATTCTAA+TGG | 0.303527 | 1.2:-28549630 | MS.gene53563:CDS |
| GAATCCTTTACTCACAATAT+TGG | 0.308722 | 1.2:+28560916 | None:intergenic |
| AACGCTTTATACCAAATATA+TGG | 0.309972 | 1.2:-28554133 | MS.gene53563:CDS |
| ACGTCCAAGTGGGCAGAATT+TGG | 0.331384 | 1.2:-28561384 | MS.gene53563:CDS |
| ACCACACTGCTTCCTGTTCC+CGG | 0.334290 | 1.2:+28554165 | None:intergenic |
| CCATATACATAGGGGTTTCC+TGG | 0.337764 | 1.2:+28562858 | None:intergenic |
| TTCTGAAGAATATGTCTTCA+TGG | 0.338718 | 1.2:+28561850 | None:intergenic |
| CACAACAGGGCAACCAGCTT+TGG | 0.339516 | 1.2:+28563915 | None:intergenic |
| GCAAAAGGGACTTGCATTGC+AGG | 0.339981 | 1.2:-28549972 | MS.gene53563:CDS |
| ATTTGTGCTGCTGCACTACT+TGG | 0.350396 | 1.2:-28551741 | MS.gene53563:CDS |
| TCTACAAAGTTCAATATGAT+TGG | 0.351830 | 1.2:+28558888 | None:intergenic |
| TCTTGATAGAAGTGGCTGTT+TGG | 0.356538 | 1.2:-28549934 | MS.gene53563:CDS |
| CCTATGTATATGGACTTATT+GGG | 0.357493 | 1.2:-28562848 | MS.gene53563:CDS |
| TCTGCTATGTTGAACCAATA+TGG | 0.360428 | 1.2:+28551855 | None:intergenic |
| AACTGTGTCTTCGGGAAATA+GGG | 0.360832 | 1.2:-28561116 | MS.gene53563:CDS |
| GGATAAGTGAACCTCCAAAT+TGG | 0.360993 | 1.2:+28534113 | None:intergenic |
| TTCCTTTATGTAACTGAGTT+CGG | 0.363823 | 1.2:+28544191 | None:intergenic |
| ACAACAGGGCAACCAGCTTT+GGG | 0.369360 | 1.2:+28563916 | None:intergenic |
| TCCTTATGTGAAACTTCAAT+TGG | 0.371476 | 1.2:+28549546 | None:intergenic |
| GGGGTACCAGTCCATATATT+TGG | 0.374561 | 1.2:+28554122 | None:intergenic |
| CCTCCAAATTGGTTCAGATA+TGG | 0.379373 | 1.2:+28534124 | None:intergenic |
| GGAGTTCTGCTATAAAATCT+TGG | 0.386208 | 1.2:+28549496 | None:intergenic |
| CAGTGTAACATGCTCAATTC+CGG | 0.387233 | 1.2:-28559903 | MS.gene53563:CDS |
| TCCAATTGAAGTTTCACATA+AGG | 0.387461 | 1.2:-28549547 | MS.gene53563:CDS |
| TCGTAACGTAACTGTGTCTT+CGG | 0.392745 | 1.2:-28561125 | MS.gene53563:CDS |
| AGGGAGAAATATCTTATTCC+CGG | 0.394099 | 1.2:-28554184 | MS.gene53563:CDS |
| TAGTTGGTTTATTACCATAT+TGG | 0.395583 | 1.2:-28551869 | MS.gene53563:CDS |
| CCTACGTAGAGTTTCTTATC+TGG | 0.396333 | 1.2:+28549360 | None:intergenic |
| TTTGGTTCTCAGGTATGTTA+GGG | 0.397494 | 1.2:-28560143 | MS.gene53563:intron |
| TGCAGAAAACTATGCAAAAC+AGG | 0.403387 | 1.2:-28549670 | MS.gene53563:CDS |
| GCAGTGTTCTTGATGTTTAG+TGG | 0.405353 | 1.2:+28549873 | None:intergenic |
| GTGAAGGGAGAAGAAGGAAA+TGG | 0.407787 | 1.2:-28552777 | MS.gene53563:CDS |
| ATATACTACCTCAAGCTTCT+GGG | 0.410554 | 1.2:+28552732 | None:intergenic |
| ATGGGCACAACTTGCATTGA+TGG | 0.412451 | 1.2:-28549840 | MS.gene53563:CDS |
| AGGGCTCAACCTTTAACTCT+TGG | 0.413129 | 1.2:-28561097 | MS.gene53563:CDS |
| CAAGCTGGCTGCATCGTTCA+AGG | 0.416807 | 1.2:+28536523 | None:intergenic |
| TAAAGAAACACACCTTGATA+AGG | 0.417802 | 1.2:+28554004 | None:intergenic |
| TGTTTAATTTGTTCCTCTCC+AGG | 0.418327 | 1.2:+28554101 | None:intergenic |
| TGGAATACCTTCTTTGTTGA+AGG | 0.419005 | 1.2:-28560968 | MS.gene53563:CDS |
| TTTCTGGGCTGTTTCTGGTT+CGG | 0.420161 | 1.2:+28536604 | None:intergenic |
| GAACTTCGATCCATCATTGC+TGG | 0.422226 | 1.2:-28550050 | MS.gene53563:CDS |
| AACAAGCACAAGGCTCCTTT+CGG | 0.426958 | 1.2:+28550022 | None:intergenic |
| ATCGAACCATTCAGAATGTC+TGG | 0.427480 | 1.2:+28561329 | None:intergenic |
| TTGACCAAAAGATCAGAAAC+AGG | 0.429669 | 1.2:+28561300 | None:intergenic |
| TTCGGTGGTTCCAGCAATGA+TGG | 0.430365 | 1.2:+28550040 | None:intergenic |
| CTTGATTTACCACCACCATT+TGG | 0.431025 | 1.2:+28551777 | None:intergenic |
| TTCAATATTTCATTGAGCTC+AGG | 0.431501 | 1.2:+28558823 | None:intergenic |
| GGAGACTTCTATGAAGCTCT+TGG | 0.433758 | 1.2:-28564009 | MS.gene53563:CDS |
| ACATAGGTAAAGGAGCTCTA+TGG | 0.435253 | 1.2:-28561169 | MS.gene53563:intron |
| AATATCTTATTCCCGGGAAC+AGG | 0.435984 | 1.2:-28554177 | MS.gene53563:CDS |
| TGAAGGGAGAAGAAGGAAAT+GGG | 0.436793 | 1.2:-28552776 | MS.gene53563:CDS |
| TTGAGAATATGGAGTCTGTA+TGG | 0.437197 | 1.2:-28554454 | MS.gene53563:CDS |
| CCTACTACAGGCATAGGTTC+TGG | 0.437803 | 1.2:+28562804 | None:intergenic |
| TTCACCAAATTCTGCCCACT+TGG | 0.439880 | 1.2:+28561380 | None:intergenic |
| GTCTCAACTTAGAGCTCCTA+AGG | 0.447138 | 1.2:-28554204 | MS.gene53563:CDS |
| TCAGAATCTTAGGCAAACTT+TGG | 0.447427 | 1.2:-28563368 | MS.gene53563:intron |
| CAATCATTATTTCTATTGAC+AGG | 0.448373 | 1.2:-28551801 | MS.gene53563:CDS |
| TATTTCTGCATACATCTTTG+AGG | 0.450139 | 1.2:-28561769 | MS.gene53563:CDS |
| GAGATTGCATCCAAAATTCA+AGG | 0.450407 | 1.2:-28560094 | MS.gene53563:intron |
| TATGCGGGTTTAAATCCCTT+TGG | 0.450508 | 1.2:-28563961 | MS.gene53563:CDS |
| CTCAGGAATTCCTTCCCTCT+TGG | 0.450796 | 1.2:+28549752 | None:intergenic |
| AACTACATTCTCCCCACAAC+AGG | 0.458392 | 1.2:+28561352 | None:intergenic |
| TTGAAGAGCCTATAGTCATC+GGG | 0.460296 | 1.2:+28549403 | None:intergenic |
| AAACATGCTTTAGATTCTAA+AGG | 0.460656 | 1.2:-28554074 | MS.gene53563:CDS |
| TCTTGGTTCGAGCTTGAGCT+GGG | 0.468735 | 1.2:+28563081 | None:intergenic |
| GAATAAGATATTTCTCCCTT+AGG | 0.469726 | 1.2:+28554188 | None:intergenic |
| GATTCTAAAGGTAGAAAGGT+AGG | 0.469843 | 1.2:-28554062 | MS.gene53563:CDS |
| CGAGGAACAGAGACTGCAAA+AGG | 0.471698 | 1.2:-28549987 | MS.gene53563:CDS |
| AGCAATGATGGATCGAAGTT+CGG | 0.472676 | 1.2:+28550052 | None:intergenic |
| GTCCCACGTGGGTGGCTACT+GGG | 0.473923 | 1.2:-28558789 | MS.gene53563:CDS |
| GCTCTTTGGATAACAATCTC+AGG | 0.474099 | 1.2:+28549735 | None:intergenic |
| TCAATGGTAATAATTCTAAT+GGG | 0.475596 | 1.2:-28549629 | MS.gene53563:CDS |
| AGCCGAACTCAGTTACATAA+AGG | 0.475710 | 1.2:-28544193 | MS.gene53563:CDS |
| AATCGCAAGATGAAGATAGT+TGG | 0.476644 | 1.2:-28551885 | MS.gene53563:CDS |
| TTTAGATTCTAAAGGTAGAA+AGG | 0.482930 | 1.2:-28554066 | MS.gene53563:CDS |
| CACATGAGCATATAACGCTT+TGG | 0.483246 | 1.2:+28553767 | None:intergenic |
| CCAATAAGTCCATATACATA+GGG | 0.484730 | 1.2:+28562849 | None:intergenic |
| AACGATGCAGCCAGCTTGAA+AGG | 0.487651 | 1.2:-28536518 | MS.gene53563:intron |
| GAAGAAACACACGTAAATTC+CGG | 0.487982 | 1.2:+28559884 | None:intergenic |
| TTAAAGACCGGAAAGTTTCA+AGG | 0.488874 | 1.2:-28564794 | MS.gene53563:CDS |
| TCTTAATTGGCACTAGGGAA+TGG | 0.489576 | 1.2:-28549431 | MS.gene53563:CDS |
| CGTAACGTAACTGTGTCTTC+GGG | 0.490069 | 1.2:-28561124 | MS.gene53563:CDS |
| AGGAATTTCTCATTCGGCAA+GGG | 0.490800 | 1.2:-28561900 | MS.gene53563:CDS |
| GCTGCACTACTTGGGATATG+TGG | 0.491552 | 1.2:-28551732 | MS.gene53563:CDS |
| GGAATCACGGCGGATTCAGC+AGG | 0.494688 | 1.2:+28551699 | None:intergenic |
| AACACTGCACACAAAGCAAT+GGG | 0.495188 | 1.2:-28549858 | MS.gene53563:CDS |
| GAGACTTCTATGAAGCTCTT+GGG | 0.497556 | 1.2:-28564008 | MS.gene53563:CDS |
| GATTGACGAAGTGATATCTC+TGG | 0.499713 | 1.2:-28554531 | MS.gene53563:CDS |
| TTGGATGCAATCTCATAAGA+AGG | 0.501103 | 1.2:+28560103 | None:intergenic |
| GTTTAATTTGTTCCTCTCCA+GGG | 0.501339 | 1.2:+28554102 | None:intergenic |
| ATGGACAAACAAAACCTACT+TGG | 0.502117 | 1.2:-28549821 | MS.gene53563:CDS |
| AGCATGATAGAGTCAAAATA+GGG | 0.503339 | 1.2:+28551678 | None:intergenic |
| CCCAATAAGTCCATATACAT+AGG | 0.504850 | 1.2:+28562848 | None:intergenic |
| ACTTTCAGGCATGCTCATCC+AGG | 0.506081 | 1.2:-28562876 | MS.gene53563:intron |
| AAGTAAGCACATCATTTATG+TGG | 0.507055 | 1.2:+28554411 | None:intergenic |
| ATCTGATCGTAGACCGCCAA+AGG | 0.508138 | 1.2:+28563945 | None:intergenic |
| TTGTATTCTTGTTGAATATG+CGG | 0.508271 | 1.2:-28563977 | MS.gene53563:CDS |
| GTGTTTCTTAATTGGCACTA+GGG | 0.508539 | 1.2:-28549436 | MS.gene53563:CDS |
| CCACACTGCTTCCTGTTCCC+GGG | 0.508620 | 1.2:+28554166 | None:intergenic |
| GTTCTTGATGTTTAGTGGCA+AGG | 0.508692 | 1.2:+28549878 | None:intergenic |
| CCAGAACCTATGCCTGTAGT+AGG | 0.508789 | 1.2:-28562804 | MS.gene53563:intron |
| GCGGGTTTAAATCCCTTTGG+CGG | 0.509226 | 1.2:-28563958 | MS.gene53563:CDS |
| TCGTCTTGCCATGTTACCAT+TGG | 0.509525 | 1.2:-28544157 | MS.gene53563:CDS |
| ACAGGAAGCAGTGTGGTTCA+AGG | 0.510474 | 1.2:-28554159 | MS.gene53563:CDS |
| TTCTGGGATTCTACTAATGT+AGG | 0.510720 | 1.2:+28552748 | None:intergenic |
| TGTATTCTTGTTGAATATGC+GGG | 0.511487 | 1.2:-28563976 | MS.gene53563:CDS |
| AGTGTTTCTTAATTGGCACT+AGG | 0.513671 | 1.2:-28549437 | MS.gene53563:CDS |
| TTTCTATTGACAGGACCAAA+TGG | 0.518729 | 1.2:-28551792 | MS.gene53563:CDS |
| TTTAATTTGTTCCTCTCCAG+GGG | 0.519994 | 1.2:+28554103 | None:intergenic |
| AGTAAGACCTTCAACAAAGA+AGG | 0.520985 | 1.2:+28560961 | None:intergenic |
| CCATATCTGAACCAATTTGG+AGG | 0.523989 | 1.2:-28534124 | MS.gene53563:CDS |
| AGGTGGAGGCCGCCTTATCA+AGG | 0.528089 | 1.2:-28554016 | MS.gene53563:intron |
| CTTGCAGTGAAGGGAGAAGA+AGG | 0.529921 | 1.2:-28552783 | MS.gene53563:intron |
| CTTAACAGGAAGCCTCGATT+CGG | 0.532794 | 1.2:+28548567 | None:intergenic |
| TACGTGTGTTTCTTCAGCAA+AGG | 0.535657 | 1.2:-28559876 | MS.gene53563:intron |
| TCAGGTGTCATTCCGACGGA+TGG | 0.539396 | 1.2:-28560988 | MS.gene53563:intron |
| TTTGAAACTGCCAAGAGGGA+AGG | 0.541249 | 1.2:-28549762 | MS.gene53563:CDS |
| CAAAGCTGGTTGCCCTGTTG+TGG | 0.541775 | 1.2:-28563914 | MS.gene53563:intron |
| GTAGTTCCAGACATTCTGAA+TGG | 0.543495 | 1.2:-28561335 | MS.gene53563:CDS |
| TCAGATAGCATCCCCAAAGC+TGG | 0.543781 | 1.2:-28563928 | MS.gene53563:CDS |
| ATTGAAACTCTTGATAGAAG+TGG | 0.546230 | 1.2:-28549942 | MS.gene53563:CDS |
| TTCATCAGGAATAGCAGAGA+AGG | 0.546650 | 1.2:+28554481 | None:intergenic |
| AATTGAAGTTTCACATAAGG+AGG | 0.549162 | 1.2:-28549544 | MS.gene53563:CDS |
| TATTAGTTCCAATGGTAACA+TGG | 0.549852 | 1.2:+28544149 | None:intergenic |
| TAAGAGAGATTGAGCAGGAA+GGG | 0.550637 | 1.2:-28536631 | MS.gene53563:CDS |
| AGACATTGACTTCAAAACCT+TGG | 0.551104 | 1.2:-28558764 | MS.gene53563:intron |
| CCATCTTGCAGCATCCAAGT+AGG | 0.551358 | 1.2:+28549807 | None:intergenic |
| CTTGAAGAGCCTATAGTCAT+CGG | 0.553092 | 1.2:+28549402 | None:intergenic |
| TGCTAAGCTACTTGAATGGA+AGG | 0.553515 | 1.2:-28558914 | MS.gene53563:CDS |
| TTTGTGCTGCTGCACTACTT+GGG | 0.553681 | 1.2:-28551740 | MS.gene53563:CDS |
| TGTATTGTGGAGGAAATTCA+AGG | 0.555964 | 1.2:-28563104 | MS.gene53563:CDS |
| GTCCTCAGCTGCTTTGTCCA+CGG | 0.556624 | 1.2:+28554388 | None:intergenic |
| ATAAGCTCCTCCTTATCTGA+TGG | 0.557752 | 1.2:+28534092 | None:intergenic |
| GTTCACTTATCCATCAGATA+AGG | 0.558311 | 1.2:-28534102 | MS.gene53563:CDS |
| CAGGAAGCAGTGTGGTTCAA+GGG | 0.561932 | 1.2:-28554158 | MS.gene53563:CDS |
| CCTACTTGGATGCTGCAAGA+TGG | 0.562471 | 1.2:-28549807 | MS.gene53563:CDS |
| TTCTGCATACATCTTTGAGG+AGG | 0.564382 | 1.2:-28561766 | MS.gene53563:CDS |
| TTTATACCAAATATATGGAC+TGG | 0.566964 | 1.2:-28554128 | MS.gene53563:CDS |
| AAGAGGAAAACCTTTCAAGC+TGG | 0.567212 | 1.2:+28536508 | None:intergenic |
| AACATTTAATTCGTCCCACG+TGG | 0.567215 | 1.2:-28558801 | MS.gene53563:CDS |
| TTGATAGAAGTGGCTGTTTG+GGG | 0.567646 | 1.2:-28549932 | MS.gene53563:CDS |
| TAAAGACCGGAAAGTTTCAA+GGG | 0.568388 | 1.2:-28564793 | MS.gene53563:CDS |
| GTAGAAAGGTAGGTGAGGAA+TGG | 0.569613 | 1.2:-28554052 | MS.gene53563:CDS |
| GGGAGAAATATCTTATTCCC+GGG | 0.571092 | 1.2:-28554183 | MS.gene53563:CDS |
| GAATTGTCTAGTTACATCAA+TGG | 0.571307 | 1.2:-28549645 | MS.gene53563:CDS |
| TAGGAATTTCTCATTCGGCA+AGG | 0.571429 | 1.2:-28561901 | MS.gene53563:CDS |
| TTGGATGATCTAACACGCAA+TGG | 0.575052 | 1.2:-28563349 | MS.gene53563:CDS |
| GAACACTGCACACAAAGCAA+TGG | 0.575474 | 1.2:-28549859 | MS.gene53563:CDS |
| ATCAACCAGCTTCCGAATCG+AGG | 0.577794 | 1.2:-28548579 | MS.gene53563:intron |
| TCTTTGAGGAGGAGTTCTTG+TGG | 0.579682 | 1.2:-28561755 | MS.gene53563:intron |
| AAATGATGTGCTTACTTCCG+TGG | 0.579926 | 1.2:-28554405 | MS.gene53563:CDS |
| TGGCTGCATCGTTCAAGGAA+CGG | 0.580442 | 1.2:+28536528 | None:intergenic |
| AGACATTCTGAATGGTTCGA+TGG | 0.584193 | 1.2:-28561327 | MS.gene53563:CDS |
| AAGTGGGCAGAATTTGGTGA+AGG | 0.584310 | 1.2:-28561378 | MS.gene53563:CDS |
| CACTTATCCATCAGATAAGG+AGG | 0.584631 | 1.2:-28534099 | MS.gene53563:CDS |
| TTCCGTGGACAAAGCAGCTG+AGG | 0.588630 | 1.2:-28554390 | MS.gene53563:CDS |
| AATATATGGACTGGTACCCC+TGG | 0.588784 | 1.2:-28554119 | MS.gene53563:CDS |
| TTACTTCCACCACATTGCAG+TGG | 0.589221 | 1.2:-28560943 | MS.gene53563:CDS |
| GTGGTAAATCAAGTTTACTT+CGG | 0.593011 | 1.2:-28551767 | MS.gene53563:CDS |
| AGAGTCAAAATAGGGAATCA+CGG | 0.597474 | 1.2:+28551686 | None:intergenic |
| TAAAGGTAGAAAGGTAGGTG+AGG | 0.597978 | 1.2:-28554057 | MS.gene53563:CDS |
| TGGGCAGAATTTGGTGAAGG+GGG | 0.598874 | 1.2:-28561375 | MS.gene53563:CDS |
| CTGTTAAGCAATATAGCTGA+CGG | 0.601314 | 1.2:-28548552 | MS.gene53563:intron |
| TCAGGTAAGAGAGATTGAGC+AGG | 0.601613 | 1.2:-28536636 | MS.gene53563:intron |
| AAGCACAAGGCTCCTTTCGG+TGG | 0.602348 | 1.2:+28550025 | None:intergenic |
| AGTGGGCAGAATTTGGTGAA+GGG | 0.606015 | 1.2:-28561377 | MS.gene53563:CDS |
| TTCATACCTACTACAGGCAT+AGG | 0.606169 | 1.2:+28562798 | None:intergenic |
| GGAATTTCTCATTCGGCAAG+GGG | 0.606177 | 1.2:-28561899 | MS.gene53563:CDS |
| GCTCTATGGTCTTGATCATG+AGG | 0.608538 | 1.2:-28561155 | MS.gene53563:CDS |
| AGATTGCTAAGCTACTTGAA+TGG | 0.611550 | 1.2:-28558918 | MS.gene53563:CDS |
| TATTGGTTCAACATAGCAGA+AGG | 0.611717 | 1.2:-28551852 | MS.gene53563:CDS |
| TTTGTCTTGACAGTGTATTG+TGG | 0.613295 | 1.2:-28563117 | MS.gene53563:intron |
| GAGGAACAGAGACTGCAAAA+GGG | 0.616780 | 1.2:-28549986 | MS.gene53563:CDS |
| GTAAGAGAGATTGAGCAGGA+AGG | 0.617360 | 1.2:-28536632 | MS.gene53563:CDS |
| GTGGGCAGAATTTGGTGAAG+GGG | 0.618082 | 1.2:-28561376 | MS.gene53563:CDS |
| AGTAGAATCCCAGAAGCTTG+AGG | 0.618095 | 1.2:-28552740 | MS.gene53563:intron |
| TTTAATTCGTCCCACGTGGG+TGG | 0.619599 | 1.2:-28558797 | MS.gene53563:CDS |
| ATGGTTTACCACTACGAAGG+TGG | 0.620328 | 1.2:-28554033 | MS.gene53563:CDS |
| CTATGTATATGGACTTATTG+GGG | 0.620912 | 1.2:-28562847 | MS.gene53563:CDS |
| CAACAGGGCAACCAGCTTTG+GGG | 0.623060 | 1.2:+28563917 | None:intergenic |
| CGTCTCAGGTGTCATTCCGA+CGG | 0.627015 | 1.2:-28560992 | MS.gene53563:intron |
| TTGACAGGACCAAATGGTGG+TGG | 0.627465 | 1.2:-28551786 | MS.gene53563:CDS |
| AATATGGAGTCTGTATGGAA+AGG | 0.632058 | 1.2:-28554449 | MS.gene53563:CDS |
| AAAGAAGGTATTCCATCCGT+CGG | 0.632398 | 1.2:+28560976 | None:intergenic |
| GATGCAATCTCATAAGAAGG+AGG | 0.635557 | 1.2:+28560106 | None:intergenic |
| TCTGATCGTAGACCGCCAAA+GGG | 0.635573 | 1.2:+28563946 | None:intergenic |
| GTCTTGACAGTGTATTGTGG+AGG | 0.636667 | 1.2:-28563114 | MS.gene53563:intron |
| GTACCATGAAGCAAATGTCA+AGG | 0.637451 | 1.2:-28553886 | MS.gene53563:CDS |
| GACGAAGTGATATCTCTGGA+TGG | 0.641344 | 1.2:-28554527 | MS.gene53563:CDS |
| ATGGACTGGTACCCCTGGAG+AGG | 0.642746 | 1.2:-28554114 | MS.gene53563:CDS |
| GTTTACCACTACGAAGGTGG+AGG | 0.643110 | 1.2:-28554030 | MS.gene53563:CDS |
| AAGCGTTATATGCTCATGTG+AGG | 0.646209 | 1.2:-28553764 | MS.gene53563:intron |
| TCTCAACTTAGAGCTCCTAA+GGG | 0.649001 | 1.2:-28554203 | MS.gene53563:CDS |
| ATGCAATCTCATAAGAAGGA+GGG | 0.653021 | 1.2:+28560107 | None:intergenic |
| CTATTGACAGGACCAAATGG+TGG | 0.654961 | 1.2:-28551789 | MS.gene53563:CDS |
| AGAAACACACCTTGATAAGG+CGG | 0.656077 | 1.2:+28554007 | None:intergenic |
| AACACGCAATGGTTATTCAG+TGG | 0.656780 | 1.2:-28563338 | MS.gene53563:intron |
| AATATGTCTTCATGGTCTCG+AGG | 0.658332 | 1.2:+28561858 | None:intergenic |
| TAAGAAACTCTACGTAGGAG+AGG | 0.665205 | 1.2:-28549355 | MS.gene53563:intron |
| AAAGACCGGAAAGTTTCAAG+GGG | 0.668254 | 1.2:-28564792 | MS.gene53563:CDS |
| AGGAGCTCTAAGTTGAGACA+TGG | 0.668968 | 1.2:+28554208 | None:intergenic |
| TCATTGCTGGAACCACCGAA+AGG | 0.673113 | 1.2:-28550037 | MS.gene53563:CDS |
| TTATCAGGAATTTCTCACGA+GGG | 0.673961 | 1.2:-28549585 | MS.gene53563:CDS |
| ACATTTAATTCGTCCCACGT+GGG | 0.675111 | 1.2:-28558800 | MS.gene53563:CDS |
| CAATAAGTCCATATACATAG+GGG | 0.675168 | 1.2:+28562850 | None:intergenic |
| TGGTTCAACATAGCAGAAGG+AGG | 0.676802 | 1.2:-28551849 | MS.gene53563:CDS |
| TGTATATGGACTTATTGGGG+CGG | 0.677499 | 1.2:-28562844 | MS.gene53563:CDS |
| TTTATCAGGAATTTCTCACG+AGG | 0.678520 | 1.2:-28549586 | MS.gene53563:CDS |
| GGCGGCCTCCACCTTCGTAG+TGG | 0.679029 | 1.2:+28554025 | None:intergenic |
| TATTTCAGGAACGTCCAAGT+GGG | 0.679077 | 1.2:-28561394 | MS.gene53563:intron |
| TAAGCTACTTGAATGGAAGG+AGG | 0.685452 | 1.2:-28558911 | MS.gene53563:CDS |
| TGAAGAGCCTATAGTCATCG+GGG | 0.689448 | 1.2:+28549404 | None:intergenic |
| GGTAATCCACTGCAATGTGG+TGG | 0.696559 | 1.2:+28560937 | None:intergenic |
| AGACACGAAGCAGACAGCTG+CGG | 0.697819 | 1.2:-28536579 | MS.gene53563:CDS |
| GAAGAGCCTATAGTCATCGG+GGG | 0.702345 | 1.2:+28549405 | None:intergenic |
| ATATTTCATCAACAAGCACA+AGG | 0.705941 | 1.2:+28550012 | None:intergenic |
| GTATTTCAGGAACGTCCAAG+TGG | 0.706411 | 1.2:-28561395 | MS.gene53563:intron |
| AAGACCGGAAAGTTTCAAGG+GGG | 0.715418 | 1.2:-28564791 | MS.gene53563:CDS |
| TTTGGTAATCACAAGCAATG+TGG | 0.717978 | 1.2:+28553785 | None:intergenic |
| TAACCCAGTAGCCACCCACG+TGG | 0.719610 | 1.2:+28558786 | None:intergenic |
| CCCGGGAACAGGAAGCAGTG+TGG | 0.720310 | 1.2:-28554166 | MS.gene53563:CDS |
| CCAGATAAGAAACTCTACGT+AGG | 0.723183 | 1.2:-28549360 | MS.gene53563:CDS |
| TGACCAAAAGATCAGAAACA+GGG | 0.725618 | 1.2:+28561301 | None:intergenic |
| GGAATGGTTTACCACTACGA+AGG | 0.726194 | 1.2:-28554036 | MS.gene53563:CDS |
| GAATTTCTCATTCGGCAAGG+GGG | 0.728126 | 1.2:-28561898 | MS.gene53563:CDS |
| AAAATGCCAATACCACAACA+GGG | 0.735670 | 1.2:+28563902 | None:intergenic |
| CTTGTTGATGAAATATGCCG+AGG | 0.737644 | 1.2:-28550005 | MS.gene53563:CDS |
| AACCCAGTAGCCACCCACGT+GGG | 0.758606 | 1.2:+28558787 | None:intergenic |
| ATTGGTAATCCACTGCAATG+TGG | 0.767045 | 1.2:+28560934 | None:intergenic |
| GTCAAAATAGGGAATCACGG+CGG | 0.796421 | 1.2:+28551689 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 28534073 | 28564843 | 28534073 | ID=MS.gene53563 |
| chr1.2 | mRNA | 28534073 | 28564843 | 28534073 | ID=MS.gene53563.t1;Parent=MS.gene53563 |
| chr1.2 | exon | 28564787 | 28564843 | 28564787 | ID=MS.gene53563.t1.exon1;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28564787 | 28564843 | 28564787 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28563915 | 28564034 | 28563915 | ID=MS.gene53563.t1.exon2;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28563915 | 28564034 | 28563915 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28563339 | 28563386 | 28563339 | ID=MS.gene53563.t1.exon3;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28563339 | 28563386 | 28563339 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28563062 | 28563126 | 28563062 | ID=MS.gene53563.t1.exon4;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28563062 | 28563126 | 28563062 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28562805 | 28562890 | 28562805 | ID=MS.gene53563.t1.exon5;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28562805 | 28562890 | 28562805 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28561756 | 28561920 | 28561756 | ID=MS.gene53563.t1.exon6;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28561756 | 28561920 | 28561756 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28561299 | 28561408 | 28561299 | ID=MS.gene53563.t1.exon7;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28561299 | 28561408 | 28561299 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28561080 | 28561185 | 28561080 | ID=MS.gene53563.t1.exon8;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28561080 | 28561185 | 28561080 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28560931 | 28561006 | 28560931 | ID=MS.gene53563.t1.exon9;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28560931 | 28561006 | 28560931 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28560095 | 28560153 | 28560095 | ID=MS.gene53563.t1.exon10;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28560095 | 28560153 | 28560095 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28559877 | 28559944 | 28559877 | ID=MS.gene53563.t1.exon11;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28559877 | 28559944 | 28559877 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28558765 | 28558938 | 28558765 | ID=MS.gene53563.t1.exon12;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28558765 | 28558938 | 28558765 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28554376 | 28554582 | 28554376 | ID=MS.gene53563.t1.exon13;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28554376 | 28554582 | 28554376 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28554017 | 28554273 | 28554017 | ID=MS.gene53563.t1.exon14;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28554017 | 28554273 | 28554017 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28553765 | 28553908 | 28553765 | ID=MS.gene53563.t1.exon15;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28553765 | 28553908 | 28553765 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28552741 | 28552798 | 28552741 | ID=MS.gene53563.t1.exon16;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28552741 | 28552798 | 28552741 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28551629 | 28551928 | 28551629 | ID=MS.gene53563.t1.exon17;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28551629 | 28551928 | 28551629 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28549356 | 28550084 | 28549356 | ID=MS.gene53563.t1.exon18;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28549356 | 28550084 | 28549356 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28548553 | 28548592 | 28548553 | ID=MS.gene53563.t1.exon19;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28548553 | 28548592 | 28548553 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28544143 | 28544213 | 28544143 | ID=MS.gene53563.t1.exon20;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28544143 | 28544213 | 28544143 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28536519 | 28536654 | 28536519 | ID=MS.gene53563.t1.exon21;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28536519 | 28536654 | 28536519 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| chr1.2 | exon | 28534073 | 28534146 | 28534073 | ID=MS.gene53563.t1.exon22;Parent=MS.gene53563.t1 |
| chr1.2 | CDS | 28534073 | 28534146 | 28534073 | ID=cds.MS.gene53563.t1;Parent=MS.gene53563.t1 |
| Gene Sequence |
| Protein sequence |
