Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53753.t1 | XP_013447731.1 | 86.4 | 103 | 13 | 1 | 48 | 149 | 253 | 355 | 3.10E-26 | 128.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53753.t1 | A0A072TXB5 | 86.4 | 103 | 13 | 1 | 48 | 149 | 253 | 355 | 2.2e-26 | 128.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055359 | MS.gene53753 | 0.818599 | 1.80E-52 | -1.69E-46 |
| MS.gene058168 | MS.gene53753 | 0.80656 | 7.75E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCAATGTTATCACCAAAT+TGG | 0.217754 | 7.4:+81353853 | MS.gene53753:CDS |
| CTTCGAAGACGAAGGATTCT+AGG | 0.286350 | 7.4:+81353559 | MS.gene53753:CDS |
| TTTGAAGTTTCATCCTCATT+AGG | 0.336904 | 7.4:-81353696 | None:intergenic |
| TTATTATTAGCATCCCAATT+TGG | 0.341400 | 7.4:-81353867 | None:intergenic |
| TCATGAGAATCACTATTATT+AGG | 0.353829 | 7.4:-81353828 | None:intergenic |
| TCCAATGTTATCACCAAATT+GGG | 0.411724 | 7.4:+81353854 | MS.gene53753:CDS |
| CTAAAGATAGAAAGATGAAA+AGG | 0.414926 | 7.4:+81353523 | MS.gene53753:CDS |
| TCTTGATATTGCAGAAAAGT+TGG | 0.424180 | 7.4:-81353916 | None:intergenic |
| AATAACAATGATGCTGTCAC+TGG | 0.496363 | 7.4:+81353888 | MS.gene53753:CDS |
| CATTAGGCATCAATTGATGA+AGG | 0.499616 | 7.4:-81353680 | None:intergenic |
| TCAAGAACGAATCTATCATC+AGG | 0.510472 | 7.4:+81353933 | MS.gene53753:CDS |
| AAAGATGAAAAGGGCACAGA+AGG | 0.517893 | 7.4:+81353533 | MS.gene53753:CDS |
| TAAAGATAGAAAGATGAAAA+GGG | 0.526209 | 7.4:+81353524 | MS.gene53753:CDS |
| AGAAAACAAGAGCAAGAGCA+AGG | 0.530089 | 7.4:+81353583 | MS.gene53753:CDS |
| TCATCAATTGATGCCTAATG+AGG | 0.536962 | 7.4:+81353683 | MS.gene53753:CDS |
| TTCGAAGACGAAGGATTCTA+GGG | 0.537014 | 7.4:+81353560 | MS.gene53753:CDS |
| TGATATCTTTAACTTCATTG+AGG | 0.548363 | 7.4:+81353734 | MS.gene53753:CDS |
| TCCCAATTTGGTGATAACAT+TGG | 0.563066 | 7.4:-81353855 | None:intergenic |
| GAAAACAAGAGCAAGAGCAA+GGG | 0.591086 | 7.4:+81353584 | MS.gene53753:CDS |
| GAAGGAATCTTCGAAGACGA+AGG | 0.639563 | 7.4:+81353551 | MS.gene53753:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAGATAGAAAGATGAAAA+GGG | + | chr7.4:81353524-81353543 | MS.gene53753:CDS | 20.0% |
| ! | CTAAAGATAGAAAGATGAAA+AGG | + | chr7.4:81353523-81353542 | MS.gene53753:CDS | 25.0% |
| ! | TCATGAGAATCACTATTATT+AGG | - | chr7.4:81353831-81353850 | None:intergenic | 25.0% |
| ! | TTATTATTAGCATCCCAATT+TGG | - | chr7.4:81353870-81353889 | None:intergenic | 25.0% |
| !! | TGATATCTTTAACTTCATTG+AGG | + | chr7.4:81353734-81353753 | MS.gene53753:CDS | 25.0% |
| !!! | TGTTGATTTTGATCAATTTC+AGG | - | chr7.4:81353657-81353676 | None:intergenic | 25.0% |
| TCCAATGTTATCACCAAATT+GGG | + | chr7.4:81353854-81353873 | MS.gene53753:CDS | 30.0% | |
| TCTTGATATTGCAGAAAAGT+TGG | - | chr7.4:81353919-81353938 | None:intergenic | 30.0% | |
| ! | GCATCAAATTAGTGTTTTGT+TGG | - | chr7.4:81353809-81353828 | None:intergenic | 30.0% |
| ! | TTTGAAGTTTCATCCTCATT+AGG | - | chr7.4:81353699-81353718 | None:intergenic | 30.0% |
| AATAACAATGATGCTGTCAC+TGG | + | chr7.4:81353888-81353907 | MS.gene53753:CDS | 35.0% | |
| CATTAGGCATCAATTGATGA+AGG | - | chr7.4:81353683-81353702 | None:intergenic | 35.0% | |
| CTCCAATGTTATCACCAAAT+TGG | + | chr7.4:81353853-81353872 | MS.gene53753:CDS | 35.0% | |
| TCAAGAACGAATCTATCATC+AGG | + | chr7.4:81353933-81353952 | MS.gene53753:CDS | 35.0% | |
| TCATCAATTGATGCCTAATG+AGG | + | chr7.4:81353683-81353702 | MS.gene53753:CDS | 35.0% | |
| ! | TCCCAATTTGGTGATAACAT+TGG | - | chr7.4:81353858-81353877 | None:intergenic | 35.0% |
| AGAAAACAAGAGCAAGAGCA+AGG | + | chr7.4:81353583-81353602 | MS.gene53753:CDS | 40.0% | |
| GAAAACAAGAGCAAGAGCAA+GGG | + | chr7.4:81353584-81353603 | MS.gene53753:CDS | 40.0% | |
| TTCGAAGACGAAGGATTCTA+GGG | + | chr7.4:81353560-81353579 | MS.gene53753:CDS | 40.0% | |
| ! | AAAGATGAAAAGGGCACAGA+AGG | + | chr7.4:81353533-81353552 | MS.gene53753:CDS | 40.0% |
| CTTCGAAGACGAAGGATTCT+AGG | + | chr7.4:81353559-81353578 | MS.gene53753:CDS | 45.0% | |
| GAAGGAATCTTCGAAGACGA+AGG | + | chr7.4:81353551-81353570 | MS.gene53753:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 81353505 | 81353954 | 81353505 | ID=MS.gene53753 |
| chr7.4 | mRNA | 81353505 | 81353954 | 81353505 | ID=MS.gene53753.t1;Parent=MS.gene53753 |
| chr7.4 | exon | 81353505 | 81353954 | 81353505 | ID=MS.gene53753.t1.exon1;Parent=MS.gene53753.t1 |
| chr7.4 | CDS | 81353505 | 81353954 | 81353505 | ID=cds.MS.gene53753.t1;Parent=MS.gene53753.t1 |
| Gene Sequence |
| Protein sequence |
