Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54567.t1 | XP_013456284.1 | 85.5 | 653 | 40 | 4 | 1 | 605 | 1 | 646 | 4.50E-302 | 1046.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54567.t1 | A0A072UWT7 | 85.5 | 653 | 40 | 4 | 1 | 605 | 1 | 646 | 3.3e-302 | 1046.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene54567 | 0.800666 | 1.29E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene54567 | 0.802149 | 6.43E-49 | -1.69E-46 |
| MS.gene056368 | MS.gene54567 | 0.884381 | 2.01E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54567.t1 | MTR_4g067090 | 85.758 | 653 | 38 | 5 | 1 | 605 | 1 | 646 | 0.0 | 1114 |
| MS.gene54567.t1 | MTR_2g098830 | 50.604 | 662 | 259 | 8 | 2 | 601 | 644 | 1299 | 0.0 | 609 |
| MS.gene54567.t1 | MTR_2g098830 | 34.641 | 153 | 46 | 3 | 271 | 369 | 8 | 160 | 1.71e-16 | 84.0 |
| MS.gene54567.t1 | MTR_2g098830 | 37.143 | 140 | 80 | 3 | 341 | 477 | 510 | 644 | 2.72e-13 | 73.9 |
| MS.gene54567.t1 | MTR_2g098830 | 50.604 | 662 | 259 | 8 | 2 | 601 | 911 | 1566 | 0.0 | 609 |
| MS.gene54567.t1 | MTR_2g098830 | 55.269 | 427 | 126 | 6 | 5 | 369 | 4 | 427 | 2.66e-142 | 450 |
| MS.gene54567.t1 | MTR_2g098830 | 37.143 | 140 | 80 | 3 | 341 | 477 | 777 | 911 | 1.21e-13 | 75.1 |
| MS.gene54567.t1 | MTR_2g098710 | 64.706 | 323 | 112 | 1 | 5 | 327 | 3 | 323 | 1.57e-143 | 422 |
| MS.gene54567.t1 | MTR_2g098720 | 63.063 | 333 | 119 | 2 | 4 | 336 | 3 | 331 | 6.20e-140 | 412 |
| MS.gene54567.t1 | MTR_2g098730 | 63.072 | 306 | 111 | 1 | 5 | 310 | 4 | 307 | 2.35e-128 | 384 |
| MS.gene54567.t1 | MTR_0002s1280 | 54.925 | 335 | 106 | 4 | 5 | 336 | 4 | 296 | 7.12e-113 | 340 |
| MS.gene54567.t1 | MTR_2g098770 | 51.034 | 290 | 93 | 4 | 5 | 285 | 4 | 253 | 8.10e-83 | 265 |
| MS.gene54567.t1 | MTR_2g098740 | 52.308 | 65 | 31 | 0 | 2 | 66 | 20 | 84 | 8.96e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54567.t1 | AT1G05950 | 33.094 | 556 | 299 | 14 | 4 | 534 | 13 | 520 | 4.66e-76 | 252 |
| MS.gene54567.t1 | AT1G05950 | 33.094 | 556 | 299 | 14 | 4 | 534 | 13 | 520 | 7.61e-76 | 252 |
| MS.gene54567.t1 | AT1G05950 | 33.094 | 556 | 299 | 14 | 4 | 534 | 13 | 520 | 7.61e-76 | 252 |
| MS.gene54567.t1 | AT1G05950 | 33.094 | 556 | 299 | 14 | 4 | 534 | 13 | 520 | 2.98e-75 | 252 |
| MS.gene54567.t1 | AT1G05950 | 33.094 | 556 | 299 | 14 | 4 | 534 | 13 | 520 | 3.36e-75 | 251 |
Find 107 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGAATCTTGAAGACTTT+GGG | 0.158603 | 4.3:+37301502 | None:intergenic |
| CTGTTGAGACTTCAGAATTT+TGG | 0.161594 | 4.3:+37304442 | None:intergenic |
| CAGCTATCATTGACAGAAAA+AGG | 0.162964 | 4.3:-37304654 | MS.gene54567:CDS |
| GGTTCTGAGGCCACCTTTAT+TGG | 0.191132 | 4.3:-37304733 | MS.gene54567:CDS |
| CCTTCTTGCTGTCGATTAAA+AGG | 0.199021 | 4.3:+37304541 | None:intergenic |
| TAACACTGATATTATGCTTT+TGG | 0.222359 | 4.3:-37303882 | MS.gene54567:CDS |
| AACTTGCAAAATTCGTTAAA+TGG | 0.228149 | 4.3:+37304762 | None:intergenic |
| ATGGGCCATCGCTCGATATT+AGG | 0.237185 | 4.3:+37304579 | None:intergenic |
| TTATCTCACAGGGGATTTCT+AGG | 0.264933 | 4.3:-37303128 | MS.gene54567:intron |
| TGGCTCTGGCAATGCTATTC+AGG | 0.269758 | 4.3:-37302110 | MS.gene54567:intron |
| AAGGACAGAAACTTTCGATA+TGG | 0.276928 | 4.3:+37304560 | None:intergenic |
| GTTGAATAAACTGAGAAGTA+TGG | 0.279829 | 4.3:-37297324 | MS.gene54567:CDS |
| ATCACCAATGCTATACAATT+TGG | 0.297771 | 4.3:-37302178 | MS.gene54567:CDS |
| TGTGAGATAACTTCAGAATA+AGG | 0.310344 | 4.3:+37303141 | None:intergenic |
| GTATAGCATTGGTGATCTTC+TGG | 0.317782 | 4.3:+37302185 | None:intergenic |
| AATGGCAGATATTCTAGTTC+TGG | 0.328231 | 4.3:+37304780 | None:intergenic |
| GCATAGCTTGCAAGGTCCTT+TGG | 0.341619 | 4.3:-37303223 | MS.gene54567:intron |
| TCCAACTGAGGATGCAATTC+AGG | 0.359131 | 4.3:-37305467 | MS.gene54567:CDS |
| TCTTTGAATCTTGAAGACTT+TGG | 0.360035 | 4.3:+37301501 | None:intergenic |
| ATTTGTTCACATCCATTTCA+TGG | 0.360457 | 4.3:+37302242 | None:intergenic |
| ATCAATGATAGAATGCATTA+TGG | 0.363892 | 4.3:-37299351 | MS.gene54567:CDS |
| TCAGGTGTTTCTACAACATT+TGG | 0.364760 | 4.3:-37305449 | MS.gene54567:CDS |
| ATTTCAAACTCTGTCACTTC+AGG | 0.385082 | 4.3:+37302328 | None:intergenic |
| TGCAAGGTCCTTTGGTCAAA+AGG | 0.391451 | 4.3:-37303215 | MS.gene54567:CDS |
| TTTACAAGATTTGAGGGTAG+TGG | 0.401313 | 4.3:-37302374 | MS.gene54567:CDS |
| AATTTCATATATGCCAATAA+AGG | 0.402771 | 4.3:+37304720 | None:intergenic |
| CCTTCAGCAGCGCAAGATAG+AGG | 0.405235 | 4.3:-37300798 | MS.gene54567:CDS |
| TAACCTCCTGATTGATTGAC+TGG | 0.422854 | 4.3:+37303792 | None:intergenic |
| TATATGCTTCCAACATTACT+TGG | 0.425020 | 4.3:+37297292 | None:intergenic |
| TATTAACAGTTACATTGGCT+TGG | 0.431725 | 4.3:+37297426 | None:intergenic |
| CAAATTCTGAGAATGTTAAC+TGG | 0.435144 | 4.3:-37300752 | MS.gene54567:intron |
| CATATAGTTTACAAGATTTG+AGG | 0.436673 | 4.3:-37302381 | MS.gene54567:CDS |
| ACAGCAAGTAATTGCCAAAC+AGG | 0.437795 | 4.3:-37305371 | MS.gene54567:intron |
| CTCTATCTTGCGCTGCTGAA+GGG | 0.440040 | 4.3:+37300799 | None:intergenic |
| CCTCTATCTTGCGCTGCTGA+AGG | 0.443269 | 4.3:+37300798 | None:intergenic |
| AATAGCATTGCCAGAGCCAT+TGG | 0.446449 | 4.3:+37302114 | None:intergenic |
| TATCATGGAGACCCATGAAA+TGG | 0.452413 | 4.3:-37302254 | MS.gene54567:CDS |
| AAGCAGAAAACACACCTGTT+TGG | 0.458431 | 4.3:+37305357 | None:intergenic |
| CCTTTCAATTACATCCTTGA+GGG | 0.460597 | 4.3:+37303760 | None:intergenic |
| AGGACAGAAACTTTCGATAT+GGG | 0.463069 | 4.3:+37304561 | None:intergenic |
| TTTCAAACTCTGTCACTTCA+GGG | 0.468554 | 4.3:+37302329 | None:intergenic |
| TATACAATTTGGTGAACATC+AGG | 0.472255 | 4.3:-37302167 | MS.gene54567:CDS |
| GTTCCATCACTGAGGAGGTA+TGG | 0.478798 | 4.3:-37304497 | MS.gene54567:CDS |
| TTGAATAAACTGAGAAGTAT+GGG | 0.480086 | 4.3:-37297323 | MS.gene54567:CDS |
| TTCACCAAATTGTATAGCAT+TGG | 0.480139 | 4.3:+37302174 | None:intergenic |
| ACAAGGATGCTCAACGGTAA+AGG | 0.491827 | 4.3:-37301650 | MS.gene54567:CDS |
| TATCTTCTCTAATAAGAAAG+AGG | 0.494834 | 4.3:-37301460 | MS.gene54567:CDS |
| CAGTTTAAATCCAATGGCTC+TGG | 0.497458 | 4.3:-37302124 | MS.gene54567:CDS |
| TTATTCTGAAGTTATCTCAC+AGG | 0.498048 | 4.3:-37303139 | MS.gene54567:CDS |
| GTGCTCCCAGTCAATCAATC+AGG | 0.501419 | 4.3:-37303798 | MS.gene54567:CDS |
| TTTGAATCTTGAAGACTTTG+GGG | 0.502496 | 4.3:+37301503 | None:intergenic |
| ATATAGTTTACAAGATTTGA+GGG | 0.508018 | 4.3:-37302380 | MS.gene54567:CDS |
| TTGGATTTAAACTGCACAGA+TGG | 0.508608 | 4.3:+37302133 | None:intergenic |
| TTTGTTCACATCCATTTCAT+GGG | 0.518510 | 4.3:+37302243 | None:intergenic |
| TTCTCTTGCTTCAAGAGGAG+TGG | 0.519482 | 4.3:+37297364 | None:intergenic |
| GTTTATATTAACAGTTACAT+TGG | 0.520185 | 4.3:+37297421 | None:intergenic |
| TCTCTTGCTTCAAGAGGAGT+GGG | 0.522171 | 4.3:+37297365 | None:intergenic |
| CCCTCAAGGATGTAATTGAA+AGG | 0.522915 | 4.3:-37303760 | MS.gene54567:intron |
| TCAAAAGGAGTTCTAGCAGT+TGG | 0.526233 | 4.3:-37303200 | MS.gene54567:CDS |
| CTATTCTCAAAGCAAAGAAA+AGG | 0.537022 | 4.3:-37303846 | MS.gene54567:CDS |
| GAGTTAGAATGATTTGAACT+TGG | 0.543518 | 4.3:+37301539 | None:intergenic |
| AAGTTTAAGTGTTCCGATGA+GGG | 0.544204 | 4.3:-37297398 | MS.gene54567:CDS |
| GTAATAAATCAAGAAGGGAC+AGG | 0.545367 | 4.3:-37299308 | MS.gene54567:intron |
| ACCTGAATTGCATCCTCAGT+TGG | 0.546001 | 4.3:+37305466 | None:intergenic |
| TAGACCATGTGATTCTATCA+TGG | 0.549522 | 4.3:-37302269 | MS.gene54567:CDS |
| GCTGATTCTCTTGCTTCAAG+AGG | 0.555429 | 4.3:+37297359 | None:intergenic |
| GCAAGTTGATTGTGGTTCTG+AGG | 0.556738 | 4.3:-37304746 | MS.gene54567:CDS |
| ACCTTTCAATTACATCCTTG+AGG | 0.562218 | 4.3:+37303759 | None:intergenic |
| AATAAGAAAGAGGAATGAGC+TGG | 0.567307 | 4.3:-37301450 | MS.gene54567:intron |
| TACTTGCTGTTGAGAAGCTG+TGG | 0.567536 | 4.3:+37305384 | None:intergenic |
| GGAAGGCTGCAATGATGTAT+CGG | 0.573097 | 4.3:-37299330 | MS.gene54567:CDS |
| CTATGAAGATTGACTCGACA+AGG | 0.577961 | 4.3:-37301667 | MS.gene54567:CDS |
| CTTCAGCAGCGCAAGATAGA+GGG | 0.579807 | 4.3:-37300797 | MS.gene54567:CDS |
| TGTTGTACAACTACTACCAC+AGG | 0.579811 | 4.3:-37304812 | MS.gene54567:CDS |
| ACATGGAAGTAAACCACCAC+AGG | 0.580384 | 4.3:+37303168 | None:intergenic |
| CTTCAGAATAAGGAAGCACA+TGG | 0.581249 | 4.3:+37303151 | None:intergenic |
| GGACCATACCTCCTCAGTGA+TGG | 0.581962 | 4.3:+37304494 | None:intergenic |
| TATTCTGAAGTTATCTCACA+GGG | 0.582725 | 4.3:-37303138 | MS.gene54567:CDS |
| ATGATAGAATGCATTATGGA+AGG | 0.584757 | 4.3:-37299347 | MS.gene54567:CDS |
| GGTTATTAAAGTTCCCCTCA+AGG | 0.585039 | 4.3:-37303774 | MS.gene54567:CDS |
| TCTGTGCAGTTTAAATCCAA+TGG | 0.586104 | 4.3:-37302130 | MS.gene54567:CDS |
| TTCATATATGCCAATAAAGG+TGG | 0.587022 | 4.3:+37304723 | None:intergenic |
| GTATCGGTAATAAATCAAGA+AGG | 0.589608 | 4.3:-37299314 | MS.gene54567:CDS |
| GTTTGGTTCCATCACTGAGG+AGG | 0.590291 | 4.3:-37304502 | MS.gene54567:CDS |
| GTTCTGGATGCTGTTTCCTG+TGG | 0.591655 | 4.3:+37304796 | None:intergenic |
| CAAGTTTAAGTGTTCCGATG+AGG | 0.593108 | 4.3:-37297399 | MS.gene54567:CDS |
| TCTGCTGTGAAGGAAGCTGC+AGG | 0.595596 | 4.3:-37304324 | MS.gene54567:intron |
| CTTTCAATTACATCCTTGAG+GGG | 0.598422 | 4.3:+37303761 | None:intergenic |
| GGGACACAAGTTACCCTCAT+CGG | 0.598530 | 4.3:+37297385 | None:intergenic |
| CACTGAGGAGGTATGGTCCG+TGG | 0.602860 | 4.3:-37304490 | MS.gene54567:CDS |
| TGAAGTAGAGCTCATAGATG+TGG | 0.603411 | 4.3:-37304682 | MS.gene54567:CDS |
| AGTCGGGTATTCTGCTGTGA+AGG | 0.608334 | 4.3:-37304334 | MS.gene54567:CDS |
| AAGTTCAAATCATTCTAACT+CGG | 0.614523 | 4.3:-37301537 | MS.gene54567:CDS |
| TATCGGTAATAAATCAAGAA+GGG | 0.616879 | 4.3:-37299313 | MS.gene54567:CDS |
| TTGGACGATCACTCCTGTGG+TGG | 0.625303 | 4.3:-37303181 | MS.gene54567:CDS |
| AACCTCCTGATTGATTGACT+GGG | 0.625426 | 4.3:+37303793 | None:intergenic |
| CATGAGCAGAAAGAGAACAA+TGG | 0.627353 | 4.3:-37302304 | MS.gene54567:CDS |
| ACTTGCTGTTGAGAAGCTGT+GGG | 0.635516 | 4.3:+37305385 | None:intergenic |
| CTCCCAGTCAATCAATCAGG+AGG | 0.644545 | 4.3:-37303795 | MS.gene54567:CDS |
| GACTCGACAAGGATGCTCAA+CGG | 0.644800 | 4.3:-37301656 | MS.gene54567:CDS |
| GCTGTTTGGTTCCATCACTG+AGG | 0.651655 | 4.3:-37304505 | MS.gene54567:CDS |
| AGGATGCTCAACGGTAAAGG+CGG | 0.660364 | 4.3:-37301647 | MS.gene54567:CDS |
| CAGTTGGACGATCACTCCTG+TGG | 0.662817 | 4.3:-37303184 | MS.gene54567:CDS |
| ATGTTCCTAATATCGAGCGA+TGG | 0.664265 | 4.3:-37304584 | MS.gene54567:CDS |
| ATCAGATGTGTGTCCAACTG+AGG | 0.695565 | 4.3:-37305479 | MS.gene54567:CDS |
| GTCTCCATGATAGAATCACA+TGG | 0.715164 | 4.3:+37302265 | None:intergenic |
| ATTCTGAAGTTATCTCACAG+GGG | 0.731508 | 4.3:-37303137 | MS.gene54567:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 37297307 | 37305512 | 37297307 | ID=MS.gene54567 |
| chr4.3 | mRNA | 37297307 | 37305512 | 37297307 | ID=MS.gene54567.t1;Parent=MS.gene54567 |
| chr4.3 | exon | 37305372 | 37305512 | 37305372 | ID=MS.gene54567.t1.exon1;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37305372 | 37305512 | 37305372 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37304325 | 37304850 | 37304325 | ID=MS.gene54567.t1.exon2;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37304325 | 37304850 | 37304325 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37303761 | 37303911 | 37303761 | ID=MS.gene54567.t1.exon3;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37303761 | 37303911 | 37303761 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37303129 | 37303239 | 37303129 | ID=MS.gene54567.t1.exon4;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37303129 | 37303239 | 37303129 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37302111 | 37302414 | 37302111 | ID=MS.gene54567.t1.exon5;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37302111 | 37302414 | 37302111 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37301451 | 37301690 | 37301451 | ID=MS.gene54567.t1.exon6;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37301451 | 37301690 | 37301451 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37300753 | 37300822 | 37300753 | ID=MS.gene54567.t1.exon7;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37300753 | 37300822 | 37300753 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37299309 | 37299392 | 37299309 | ID=MS.gene54567.t1.exon8;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37299309 | 37299392 | 37299309 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| chr4.3 | exon | 37297307 | 37297497 | 37297307 | ID=MS.gene54567.t1.exon9;Parent=MS.gene54567.t1 |
| chr4.3 | CDS | 37297307 | 37297497 | 37297307 | ID=cds.MS.gene54567.t1;Parent=MS.gene54567.t1 |
| Gene Sequence |
| Protein sequence |
