Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54708.t1 | RHN49902.1 | 91.8 | 194 | 15 | 1 | 1 | 194 | 46 | 238 | 4.60E-99 | 370.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54708.t1 | Q67YE6 | 58.0 | 193 | 73 | 2 | 1 | 193 | 1 | 185 | 5.2e-59 | 228.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54708.t1 | I3SH14 | 91.8 | 194 | 15 | 1 | 1 | 194 | 1 | 193 | 3.3e-99 | 370.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene54708 | 0.852721 | 3.83E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54708.t1 | MTR_6g008210 | 92.268 | 194 | 14 | 1 | 1 | 194 | 1 | 193 | 6.64e-131 | 365 |
| MS.gene54708.t1 | MTR_0100s0160 | 49.640 | 139 | 63 | 2 | 5 | 139 | 2 | 137 | 1.76e-38 | 134 |
| MS.gene54708.t1 | MTR_7g091810 | 47.482 | 139 | 66 | 2 | 5 | 139 | 2 | 137 | 2.31e-38 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54708.t1 | AT3G28210 | 60.694 | 173 | 64 | 1 | 1 | 173 | 1 | 169 | 3.23e-72 | 216 |
| MS.gene54708.t1 | AT3G28210 | 60.694 | 173 | 64 | 1 | 1 | 173 | 1 | 169 | 3.23e-72 | 216 |
| MS.gene54708.t1 | AT3G57480 | 46.875 | 160 | 78 | 3 | 5 | 160 | 2 | 158 | 5.27e-42 | 142 |
| MS.gene54708.t1 | AT3G57480 | 46.875 | 160 | 78 | 3 | 5 | 160 | 2 | 158 | 5.27e-42 | 142 |
| MS.gene54708.t1 | AT2G41835 | 51.799 | 139 | 60 | 3 | 5 | 139 | 2 | 137 | 1.36e-40 | 139 |
| MS.gene54708.t1 | AT5G48205 | 39.091 | 110 | 34 | 2 | 5 | 108 | 2 | 84 | 1.04e-14 | 69.3 |
Find 41 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGACATTCATGTGACTTAT+AGG | 0.238124 | 6.1:-4007834 | None:intergenic |
| CTGTAAAACTTGCCACATTA+AGG | 0.277586 | 6.1:+4008060 | MS.gene54708:CDS |
| CTGCTGTTGTGGTCTGGTTT+CGG | 0.299904 | 6.1:-4007854 | None:intergenic |
| AAGGACAAATAATAGGTTTC+TGG | 0.312800 | 6.1:+4008168 | MS.gene54708:CDS |
| TTAAAGAAACACCGTAGTTC+AGG | 0.320092 | 6.1:+4007953 | MS.gene54708:CDS |
| CAAATGCAAGGACAAATAAT+AGG | 0.336795 | 6.1:+4008161 | MS.gene54708:CDS |
| TTGCAGCCATCACATGTAAA+AGG | 0.365120 | 6.1:-4007013 | None:intergenic |
| TAATAGGTTTCTGGCTGCTC+TGG | 0.375383 | 6.1:+4008177 | MS.gene54708:CDS |
| TCCTAGTAATCCTTCTGTTA+AGG | 0.396096 | 6.1:+4008258 | MS.gene54708:CDS |
| ATCTCCTTGCAACGCTTCAC+GGG | 0.399170 | 6.1:-4008013 | None:intergenic |
| AATCTCCTTGCAACGCTTCA+CGG | 0.405276 | 6.1:-4008014 | None:intergenic |
| TGGCAATGTTTACCTAAATC+TGG | 0.409207 | 6.1:-4006959 | None:intergenic |
| GCTGCTCTGGCTTCAAGAAA+TGG | 0.428865 | 6.1:+4008190 | MS.gene54708:CDS |
| AAAATTGAAACTATGGCATC+AGG | 0.440213 | 6.1:+4006920 | None:intergenic |
| GTCGGTTTCTTCTTCTTGCT+AGG | 0.441467 | 6.1:-4007986 | None:intergenic |
| TACATGTGATGGCTGCAAAC+AGG | 0.460591 | 6.1:+4007018 | MS.gene54708:CDS |
| TCGGTTTCTTCTTCTTGCTA+GGG | 0.487824 | 6.1:-4007985 | None:intergenic |
| CAACGCTTCACGGGGCAAGT+CGG | 0.495450 | 6.1:-4008004 | None:intergenic |
| TAAAGAAACACCGTAGTTCA+GGG | 0.506051 | 6.1:+4007954 | MS.gene54708:CDS |
| CGAAGAATGTTCCATGTCGA+TGG | 0.514321 | 6.1:+4007892 | MS.gene54708:CDS |
| TTAAGGTGTGCCTCAAGCAC+CGG | 0.526623 | 6.1:+4008077 | MS.gene54708:CDS |
| AAGCATGATCAGCAGCAAAC+CGG | 0.531829 | 6.1:-4008096 | None:intergenic |
| GCCTTAACAGAAGGATTACT+AGG | 0.545552 | 6.1:-4008259 | None:intergenic |
| AGACCACAACAGCAGAAAAG+TGG | 0.550028 | 6.1:+4007862 | MS.gene54708:CDS |
| ATGGAGATCACATGCAACAT+AGG | 0.562428 | 6.1:+4007911 | MS.gene54708:CDS |
| CAGCAGCAAACCGGTGCTTG+AGG | 0.563456 | 6.1:-4008087 | None:intergenic |
| TAAAAGGAAGGAAATCAAGC+TGG | 0.567522 | 6.1:-4006997 | None:intergenic |
| TGCAACAGCAGTTGTTGATG+AGG | 0.577728 | 6.1:-4008140 | None:intergenic |
| GCTGATCATGCTTGTAGTAG+AGG | 0.580042 | 6.1:+4008106 | MS.gene54708:CDS |
| CAACTGCTGTTGCAAATGCA+AGG | 0.582464 | 6.1:+4008149 | MS.gene54708:CDS |
| GCTGGTGACAATCAACATGC+TGG | 0.594811 | 6.1:-4006979 | None:intergenic |
| AATCAACAAGCCTTAACAGA+AGG | 0.596098 | 6.1:-4008268 | None:intergenic |
| TTGCCCCGTGAAGCGTTGCA+AGG | 0.600506 | 6.1:+4008009 | MS.gene54708:CDS |
| ATTGAAACTATGGCATCAGG+TGG | 0.618057 | 6.1:+4006923 | None:intergenic |
| GGTCACACTTCCCTGAACTA+CGG | 0.626391 | 6.1:-4007964 | None:intergenic |
| AGCCATCACATGTAAAAGGA+AGG | 0.626659 | 6.1:-4007009 | None:intergenic |
| GCTTGAGGCACACCTTAATG+TGG | 0.642622 | 6.1:-4008072 | None:intergenic |
| TCTCCTTGCAACGCTTCACG+GGG | 0.654277 | 6.1:-4008012 | None:intergenic |
| GCATGTGATCTCCATCGACA+TGG | 0.664435 | 6.1:-4007903 | None:intergenic |
| TTAACAGAAGGATTACTAGG+AGG | 0.691197 | 6.1:-4008256 | None:intergenic |
| CAACATAGGAGAAAACGAAG+AGG | 0.717214 | 6.1:+4007925 | MS.gene54708:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAATCTCGATTAAT+AGG | + | chr6.1:4007360-4007379 | MS.gene54708:intron | 15.0% |
| !! | CATATTTATCAAACTTAAAT+TGG | - | chr6.1:4007755-4007774 | None:intergenic | 15.0% |
| !! | TTAACATAATTTATAATGAC+AGG | + | chr6.1:4007790-4007809 | MS.gene54708:intron | 15.0% |
| !!! | TTTTTTTTTTAACAGTTTGA+TGG | - | chr6.1:4007535-4007554 | None:intergenic | 15.0% |
| !! | TGATAAATATGAAACTGAAA+AGG | + | chr6.1:4007764-4007783 | MS.gene54708:intron | 20.0% |
| !! | TGTAGTATAATCAAATATGT+TGG | + | chr6.1:4007453-4007472 | MS.gene54708:intron | 20.0% |
| ! | ATTTGACACATTTGAATATG+TGG | - | chr6.1:4007574-4007593 | None:intergenic | 25.0% |
| ! | CAGTCAAATATAGAACTAAT+CGG | - | chr6.1:4007081-4007100 | None:intergenic | 25.0% |
| ! | TAATTCATGATAACCTTCTT+GGG | + | chr6.1:4007224-4007243 | MS.gene54708:intron | 25.0% |
| ! | TATAATCAAATATGTTGGTC+TGG | + | chr6.1:4007458-4007477 | MS.gene54708:intron | 25.0% |
| ! | TTAATTCATGATAACCTTCT+TGG | + | chr6.1:4007223-4007242 | MS.gene54708:intron | 25.0% |
| !! | TCTTTATTTCTTGAAACTGA+GGG | + | chr6.1:4007161-4007180 | MS.gene54708:intron | 25.0% |
| AAGGACAAATAATAGGTTTC+TGG | + | chr6.1:4008168-4008187 | MS.gene54708:CDS | 30.0% | |
| CAAATGCAAGGACAAATAAT+AGG | + | chr6.1:4008161-4008180 | MS.gene54708:CDS | 30.0% | |
| CTCAGTTTCAAGAAATAAAG+AGG | - | chr6.1:4007162-4007181 | None:intergenic | 30.0% | |
| GTCTTTGACTAAAACAACTT+TGG | + | chr6.1:4007663-4007682 | MS.gene54708:intron | 30.0% | |
| GTTTCCAATTACAAAGTTCT+TGG | + | chr6.1:4007710-4007729 | MS.gene54708:intron | 30.0% | |
| TCACGTGATTAATAAACTTC+AGG | + | chr6.1:4007607-4007626 | MS.gene54708:intron | 30.0% | |
| TTCAAATGTGTCAAATCAAG+TGG | + | chr6.1:4007578-4007597 | MS.gene54708:intron | 30.0% | |
| TTGACTAAAACAACTTTGGT+CGG | + | chr6.1:4007667-4007686 | MS.gene54708:intron | 30.0% | |
| ! | ATAATGACAGGTATTTTGTG+TGG | + | chr6.1:4007802-4007821 | MS.gene54708:intron | 30.0% |
| ! | ATATCTCAGGTTCAATTTTC+TGG | + | chr6.1:4007294-4007313 | MS.gene54708:intron | 30.0% |
| ! | CTCTTTATTTCTTGAAACTG+AGG | + | chr6.1:4007160-4007179 | MS.gene54708:intron | 30.0% |
| ! | TGACTGAACGATTATTGATT+AGG | + | chr6.1:4007095-4007114 | MS.gene54708:intron | 30.0% |
| ATTGAACCTGAGATATTGAG+AGG | - | chr6.1:4007290-4007309 | None:intergenic | 35.0% | |
| CAAGATTGTGCCAAAAAATC+TGG | + | chr6.1:4008214-4008233 | MS.gene54708:CDS | 35.0% | |
| CTGTAAAACTTGCCACATTA+AGG | + | chr6.1:4008060-4008079 | MS.gene54708:CDS | 35.0% | |
| GCTACCAAGAACTTTGTAAT+TGG | - | chr6.1:4007717-4007736 | None:intergenic | 35.0% | |
| TAAAAGGAAGGAAATCAAGC+TGG | - | chr6.1:4007000-4007019 | None:intergenic | 35.0% | |
| TAAAGAAACACCGTAGTTCA+GGG | + | chr6.1:4007954-4007973 | MS.gene54708:CDS | 35.0% | |
| TCCTAGTAATCCTTCTGTTA+AGG | + | chr6.1:4008258-4008277 | MS.gene54708:CDS | 35.0% | |
| TGGCAATGTTTACCTAAATC+TGG | - | chr6.1:4006962-4006981 | None:intergenic | 35.0% | |
| TTAAAGAAACACCGTAGTTC+AGG | + | chr6.1:4007953-4007972 | MS.gene54708:CDS | 35.0% | |
| TTAACAGAAGGATTACTAGG+AGG | - | chr6.1:4008259-4008278 | None:intergenic | 35.0% | |
| TTTAGTCAAAGACCAAACTC+TGG | - | chr6.1:4007656-4007675 | None:intergenic | 35.0% | |
| ! | ACAGAAGCTTTTCCAGATTT+AGG | + | chr6.1:4006947-4006966 | MS.gene54708:CDS | 35.0% |
| ! | TTCCTTCCTTTTACATGTGA+TGG | + | chr6.1:4007007-4007026 | MS.gene54708:CDS | 35.0% |
| !!! | TGAACGAGATCCAGATTTTT+TGG | - | chr6.1:4008227-4008246 | None:intergenic | 35.0% |
| AGCCATCACATGTAAAAGGA+AGG | - | chr6.1:4007012-4007031 | None:intergenic | 40.0% | |
| ATGGAGATCACATGCAACAT+AGG | + | chr6.1:4007911-4007930 | MS.gene54708:CDS | 40.0% | |
| CAACATAGGAGAAAACGAAG+AGG | + | chr6.1:4007925-4007944 | MS.gene54708:CDS | 40.0% | |
| CGGACATTCATGTGACTTAT+AGG | - | chr6.1:4007837-4007856 | None:intergenic | 40.0% | |
| GCCTTAACAGAAGGATTACT+AGG | - | chr6.1:4008262-4008281 | None:intergenic | 40.0% | |
| TCGGTTTCTTCTTCTTGCTA+GGG | - | chr6.1:4007988-4008007 | None:intergenic | 40.0% | |
| TTGCAGCCATCACATGTAAA+AGG | - | chr6.1:4007016-4007035 | None:intergenic | 40.0% | |
| ! | ATGTTGGTCTGGTCAAGATT+TGG | + | chr6.1:4007469-4007488 | MS.gene54708:intron | 40.0% |
| AAGCATGATCAGCAGCAAAC+CGG | - | chr6.1:4008099-4008118 | None:intergenic | 45.0% | |
| AATCTCCTTGCAACGCTTCA+CGG | - | chr6.1:4008017-4008036 | None:intergenic | 45.0% | |
| AGACCACAACAGCAGAAAAG+TGG | + | chr6.1:4007862-4007881 | MS.gene54708:CDS | 45.0% | |
| CAACTGCTGTTGCAAATGCA+AGG | + | chr6.1:4008149-4008168 | MS.gene54708:CDS | 45.0% | |
| CGAAGAATGTTCCATGTCGA+TGG | + | chr6.1:4007892-4007911 | MS.gene54708:CDS | 45.0% | |
| CGATCAGAAACACCAGAGTT+TGG | + | chr6.1:4007641-4007660 | MS.gene54708:intron | 45.0% | |
| GCTGATCATGCTTGTAGTAG+AGG | + | chr6.1:4008106-4008125 | MS.gene54708:CDS | 45.0% | |
| TACATGTGATGGCTGCAAAC+AGG | + | chr6.1:4007018-4007037 | MS.gene54708:CDS | 45.0% | |
| TGCAACAGCAGTTGTTGATG+AGG | - | chr6.1:4008143-4008162 | None:intergenic | 45.0% | |
| TGTGCTCCTCTCAATATCTC+AGG | + | chr6.1:4007281-4007300 | MS.gene54708:intron | 45.0% | |
| ! | ACTTTTCTGCTGTTGTGGTC+TGG | - | chr6.1:4007863-4007882 | None:intergenic | 45.0% |
| ! | GTCGGTTTCTTCTTCTTGCT+AGG | - | chr6.1:4007989-4008008 | None:intergenic | 45.0% |
| !! | CAACCACTTTTCTGCTGTTG+TGG | - | chr6.1:4007868-4007887 | None:intergenic | 45.0% |
| !! | TAATAGGTTTCTGGCTGCTC+TGG | + | chr6.1:4008177-4008196 | MS.gene54708:CDS | 45.0% |
| !! | TGACCTGATGGTGTTGGTTT+CGG | + | chr6.1:4007248-4007267 | MS.gene54708:intron | 45.0% |
| ACCATCAGGTCAACCCAAGA+AGG | - | chr6.1:4007240-4007259 | None:intergenic | 50.0% | |
| ATCTCCTTGCAACGCTTCAC+GGG | - | chr6.1:4008016-4008035 | None:intergenic | 50.0% | |
| GATCCGAAACCAACACCATC+AGG | - | chr6.1:4007254-4007273 | None:intergenic | 50.0% | |
| GCATGTGATCTCCATCGACA+TGG | - | chr6.1:4007906-4007925 | None:intergenic | 50.0% | |
| GCTGGTGACAATCAACATGC+TGG | - | chr6.1:4006982-4007001 | None:intergenic | 50.0% | |
| GCTTGAGGCACACCTTAATG+TGG | - | chr6.1:4008075-4008094 | None:intergenic | 50.0% | |
| GGTCACACTTCCCTGAACTA+CGG | - | chr6.1:4007967-4007986 | None:intergenic | 50.0% | |
| TTAAGGTGTGCCTCAAGCAC+CGG | + | chr6.1:4008077-4008096 | MS.gene54708:CDS | 50.0% | |
| ! | CTGCTGTTGTGGTCTGGTTT+CGG | - | chr6.1:4007857-4007876 | None:intergenic | 50.0% |
| ! | GCTGCTCTGGCTTCAAGAAA+TGG | + | chr6.1:4008190-4008209 | MS.gene54708:CDS | 50.0% |
| !! | ACCTTCTTGGGTTGACCTGA+TGG | + | chr6.1:4007236-4007255 | MS.gene54708:intron | 50.0% |
| !! | TTGGGTTGACCTGATGGTGT+TGG | + | chr6.1:4007242-4007261 | MS.gene54708:intron | 50.0% |
| TCTCCTTGCAACGCTTCACG+GGG | - | chr6.1:4008015-4008034 | None:intergenic | 55.0% | |
| TGCACGCGCGCAAAAACTAG+AGG | + | chr6.1:4007383-4007402 | MS.gene54708:intron | 55.0% | |
| CAACGCTTCACGGGGCAAGT+CGG | - | chr6.1:4008007-4008026 | None:intergenic | 60.0% | |
| CAGCAGCAAACCGGTGCTTG+AGG | - | chr6.1:4008090-4008109 | None:intergenic | 60.0% | |
| !! | TTGCCCCGTGAAGCGTTGCA+AGG | + | chr6.1:4008009-4008028 | MS.gene54708:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 4006932 | 4008288 | 4006932 | ID=MS.gene54708 |
| chr6.1 | mRNA | 4006932 | 4008288 | 4006932 | ID=MS.gene54708.t1;Parent=MS.gene54708 |
| chr6.1 | exon | 4006932 | 4007039 | 4006932 | ID=MS.gene54708.t1.exon1;Parent=MS.gene54708.t1 |
| chr6.1 | CDS | 4006932 | 4007039 | 4006932 | ID=cds.MS.gene54708.t1;Parent=MS.gene54708.t1 |
| chr6.1 | exon | 4007812 | 4008288 | 4007812 | ID=MS.gene54708.t1.exon2;Parent=MS.gene54708.t1 |
| chr6.1 | CDS | 4007812 | 4008288 | 4007812 | ID=cds.MS.gene54708.t1;Parent=MS.gene54708.t1 |
| Gene Sequence |
| Protein sequence |
