AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54813

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54813.t1 MTR_1g069720 97.577 454 5 1 1 448 1 454 0.0 919
MS.gene54813.t1 MTR_7g097160 78.065 465 83 7 1 448 1 463 0.0 726
MS.gene54813.t1 MTR_1g075870 71.304 460 115 6 1 446 2 458 0.0 641
MS.gene54813.t1 MTR_1g042280 71.145 454 120 5 1 446 2 452 0.0 632
MS.gene54813.t1 MTR_1g075870 84.098 327 51 1 1 327 2 327 0.0 585
MS.gene54813.t1 MTR_2g099670 65.358 433 114 8 1 425 1 405 0.0 551
MS.gene54813.t1 MTR_4g126010 72.222 360 87 4 1 347 1 360 0.0 545
MS.gene54813.t1 MTR_8g018310 59.958 472 154 10 1 444 1 465 0.0 540
MS.gene54813.t1 MTR_8g018310 79.167 312 64 1 1 312 1 311 0.0 533
MS.gene54813.t1 MTR_2g039930 55.274 474 176 6 1 447 1 465 0.0 516
MS.gene54813.t1 MTR_0492s0030 68.011 372 110 4 1 370 1 365 0.0 515
MS.gene54813.t1 MTR_0543s0020 68.011 372 110 4 1 370 1 365 0.0 515
MS.gene54813.t1 MTR_1g042280 89.474 266 28 0 40 305 9 274 0.0 513
MS.gene54813.t1 MTR_6g087070 76.797 306 71 0 1 306 1 306 3.19e-179 508
MS.gene54813.t1 MTR_8g102930 77.181 298 68 0 1 298 1 298 3.98e-177 502
MS.gene54813.t1 MTR_1g075870 81.295 278 50 2 57 334 1 276 2.24e-171 482
MS.gene54813.t1 MTR_3g110180 55.582 421 171 4 1 417 1 409 3.88e-164 469
MS.gene54813.t1 MTR_3g110180 55.582 421 170 5 1 417 1 408 1.24e-162 466
MS.gene54813.t1 MTR_1g004990 70.508 295 87 0 1 295 1 295 1.71e-162 465
MS.gene54813.t1 MTR_2g099670 63.441 372 100 8 62 425 10 353 1.26e-157 452
MS.gene54813.t1 MTR_7g097160 71.118 322 74 7 144 448 25 344 4.31e-154 441
MS.gene54813.t1 MTR_2g039930 52.926 393 149 6 82 447 1 384 4.22e-136 397
MS.gene54813.t1 MTR_7g006030 37.716 289 161 6 5 277 31 316 1.21e-57 200
MS.gene54813.t1 MTR_3g110240 37.276 279 162 6 5 273 115 390 2.96e-54 192
MS.gene54813.t1 MTR_7g085540 36.525 282 163 5 9 277 147 425 4.43e-54 192
MS.gene54813.t1 MTR_1g007150 36.918 279 163 6 5 273 118 393 4.95e-54 191
MS.gene54813.t1 MTR_1g116210 36.268 284 167 6 5 277 113 393 1.45e-53 190
MS.gene54813.t1 MTR_8g014050 36.170 282 158 7 9 273 128 404 1.26e-49 179
MS.gene54813.t1 MTR_7g116400 62.687 67 24 1 80 145 1 67 9.55e-21 86.7
MS.gene54813.t1 MTR_7g106710 29.167 216 131 9 9 215 135 337 2.98e-14 74.7
MS.gene54813.t1 MTR_5g007590 45.333 75 33 1 71 145 53 119 3.62e-14 69.7
MS.gene54813.t1 MTR_5g022030 26.818 220 144 6 8 219 98 308 4.06e-14 74.7
MS.gene54813.t1 MTR_5g022030 26.818 220 144 6 8 219 98 308 4.77e-14 74.7
MS.gene54813.t1 MTR_7g106710 29.167 216 131 9 9 215 135 337 6.77e-14 73.9
MS.gene54813.t1 MTR_7g106710 29.167 216 131 9 9 215 135 337 7.40e-14 73.9
MS.gene54813.t1 MTR_4g128820 29.630 216 128 7 7 213 8 208 8.37e-14 73.6
MS.gene54813.t1 MTR_7g106710 29.167 216 131 9 9 215 135 337 1.19e-13 73.6
MS.gene54813.t1 MTR_3g466480 29.817 218 133 9 5 214 17 222 1.11e-12 70.1
MS.gene54813.t1 MTR_7g073520 34.375 128 70 4 109 236 252 365 2.51e-12 68.9
MS.gene54813.t1 MTR_1g026190 28.030 264 151 10 9 251 78 323 3.90e-12 68.6
MS.gene54813.t1 MTR_5g092815 26.609 233 148 8 9 233 91 308 9.31e-12 67.0
MS.gene54813.t1 MTR_7g106730 26.522 230 142 8 9 228 88 300 9.41e-12 67.0
MS.gene54813.t1 MTR_3g099920 28.017 232 107 9 9 213 19 217 3.30e-11 65.5
MS.gene54813.t1 MTR_5g092810 27.488 211 137 6 9 213 92 292 3.68e-11 65.5
MS.gene54813.t1 MTR_8g079790 27.948 229 139 10 6 219 122 339 4.06e-11 65.5
MS.gene54813.t1 MTR_1g098300 28.302 212 127 9 9 209 126 323 4.53e-11 65.5
MS.gene54813.t1 MTR_4g132860 25.887 282 179 10 11 281 82 344 6.35e-11 64.3
MS.gene54813.t1 MTR_8g099095 29.437 231 134 11 9 228 66 278 8.95e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54813.t1 AT4G28540 72.594 478 98 11 1 448 5 479 0.0 679
MS.gene54813.t1 AT4G14340 70.861 453 116 7 1 446 7 450 0.0 642
MS.gene54813.t1 AT3G23340 71.938 449 110 7 1 446 1 436 0.0 642
MS.gene54813.t1 AT1G03930 68.354 474 121 8 1 448 1 471 0.0 626
MS.gene54813.t1 AT5G43320 67.579 475 124 7 1 446 1 474 0.0 625
MS.gene54813.t1 AT3G23340 69.265 449 108 8 1 446 1 422 0.0 608
MS.gene54813.t1 AT1G04440 66.164 464 137 8 1 446 1 462 0.0 599
MS.gene54813.t1 AT5G44100 82.000 350 58 2 1 346 1 349 0.0 597
MS.gene54813.t1 AT5G44100 82.000 350 58 2 1 346 1 349 0.0 597
MS.gene54813.t1 AT4G14340 83.537 328 53 1 1 328 7 333 0.0 576
MS.gene54813.t1 AT5G43320 81.024 332 60 1 1 329 1 332 0.0 565
MS.gene54813.t1 AT5G43320 81.024 332 60 1 1 329 1 332 0.0 565
MS.gene54813.t1 AT1G72710 75.152 330 79 2 1 328 1 329 0.0 536
MS.gene54813.t1 AT4G26100 80.872 298 57 0 1 298 1 298 0.0 535
MS.gene54813.t1 AT5G57015 80.537 298 58 0 1 298 1 298 0.0 529
MS.gene54813.t1 AT2G19470 73.556 329 84 3 1 326 1 329 0.0 524
MS.gene54813.t1 AT4G28880 71.160 319 92 0 1 319 1 319 7.36e-176 499
MS.gene54813.t1 AT4G28880 71.160 319 92 0 1 319 1 319 7.36e-176 499
MS.gene54813.t1 AT4G28880 71.160 319 92 0 1 319 1 319 7.36e-176 499
MS.gene54813.t1 AT4G28880 71.160 319 92 0 1 319 1 319 7.36e-176 499
MS.gene54813.t1 AT4G28860 70.533 319 94 0 1 319 1 319 1.04e-174 494
MS.gene54813.t1 AT4G28860 74.832 298 75 0 1 298 1 298 1.94e-174 495
MS.gene54813.t1 AT4G08800 66.216 296 68 2 1 296 1 264 3.59e-145 416
MS.gene54813.t1 AT2G25760 39.286 280 155 4 9 273 107 386 7.13e-59 204
MS.gene54813.t1 AT2G25760 38.929 280 153 5 9 273 107 383 3.77e-56 197
MS.gene54813.t1 AT3G13670 35.986 289 166 6 5 277 131 416 3.59e-53 189
MS.gene54813.t1 AT5G18190 35.461 282 166 5 9 277 130 408 1.80e-52 187
MS.gene54813.t1 AT5G18190 35.461 282 166 5 9 277 130 408 1.80e-52 187
MS.gene54813.t1 AT3G03940 35.461 282 166 5 9 277 140 418 4.55e-52 186
MS.gene54813.t1 AT3G50530 28.319 226 141 7 5 219 144 359 1.09e-13 73.6
MS.gene54813.t1 AT5G21326 29.870 231 113 10 7 214 11 215 3.36e-13 71.6
MS.gene54813.t1 AT3G20410 28.384 229 143 8 9 230 91 305 5.44e-12 68.2
MS.gene54813.t1 AT3G20410 28.384 229 143 8 9 230 91 305 5.44e-12 68.2
MS.gene54813.t1 AT2G17890 25.909 220 146 7 8 219 107 317 5.73e-12 68.2
MS.gene54813.t1 AT1G30270 27.386 241 147 10 7 236 29 252 6.30e-12 67.8
MS.gene54813.t1 AT1G30270 27.386 241 147 10 7 236 29 252 6.46e-12 67.8
MS.gene54813.t1 AT2G38910 28.063 253 144 10 1 230 119 356 1.06e-11 67.4
MS.gene54813.t1 AT3G50530 26.104 249 148 9 5 219 144 390 1.41e-11 67.0
MS.gene54813.t1 AT4G36070 25.000 220 148 6 8 219 70 280 1.47e-11 67.0
MS.gene54813.t1 AT4G36070 25.000 220 148 6 8 219 70 280 1.79e-11 66.6
MS.gene54813.t1 AT3G61960 24.843 318 204 10 7 318 8 296 1.88e-11 66.6
MS.gene54813.t1 AT3G61960 24.843 318 204 10 7 318 8 296 2.01e-11 66.6
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT4G31170 28.319 226 128 8 8 218 130 336 2.84e-11 65.5
MS.gene54813.t1 AT1G29230 27.397 219 141 9 2 213 67 274 2.89e-11 65.9
MS.gene54813.t1 AT1G01140 28.689 244 140 13 7 236 17 240 3.10e-11 65.5
MS.gene54813.t1 AT1G01140 28.689 244 140 13 7 236 17 240 3.30e-11 65.5
MS.gene54813.t1 AT1G50700 25.597 293 192 10 9 293 73 347 4.34e-11 65.1
MS.gene54813.t1 AT1G76040 27.586 232 147 8 9 233 112 329 4.63e-11 65.1
MS.gene54813.t1 AT1G76040 28.037 214 136 7 9 215 112 314 4.65e-11 65.5
MS.gene54813.t1 AT4G23650 29.167 216 131 7 9 215 78 280 6.32e-11 64.7
MS.gene54813.t1 AT1G50700 25.597 293 192 10 9 293 73 347 6.52e-11 64.7
MS.gene54813.t1 AT4G04720 23.052 308 201 9 2 293 66 353 8.04e-11 64.3
MS.gene54813.t1 AT4G04720 23.052 308 201 9 2 293 66 353 8.04e-11 64.3
MS.gene54813.t1 AT1G50700 25.357 280 184 9 9 281 73 334 8.91e-11 64.3

Find 121 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAAACTTGGAAGGAAAAT+TGG 0.141980 1.3:-49275032 MS.gene54813:CDS
TAGAATTTGCGCGGTTCCTC+TGG 0.208716 1.3:+49267282 None:intergenic
GAATGGTCACCTGAGGAATT+TGG 0.252408 1.3:+49266735 None:intergenic
CCGACTTCAAAGTGAATTTC+CGG 0.284081 1.3:+49274173 None:intergenic
ACATACCTTCTCGAATAAAA+AGG 0.309439 1.3:+49267842 None:intergenic
CTCTTTAAATATGAATAATC+TGG 0.309704 1.3:+49267877 None:intergenic
CAAGTCATAATTACCTCTAT+TGG 0.319914 1.3:+49269185 None:intergenic
AAAATTGGGAGTGGTTCTTT+TGG 0.322856 1.3:-49275017 MS.gene54813:CDS
TTCAGTTGGGAGAGAGATTC+GGG 0.329420 1.3:-49267339 MS.gene54813:intron
CAACACAATTCAACTCGATA+TGG 0.357353 1.3:-49266792 MS.gene54813:CDS
CATAGCGTGCCGTGCCTGTA+AGG 0.359631 1.3:+49269579 None:intergenic
AATTCCTCAGGTGACCATTC+TGG 0.359748 1.3:-49266732 MS.gene54813:CDS
ATTTATTCAATTATTGTAAC+CGG 0.360177 1.3:-49274192 MS.gene54813:CDS
GTGCTAATGTATTTCTTAAG+AGG 0.362458 1.3:-49269368 MS.gene54813:CDS
TTAAACTTGGAAGGAAAATT+GGG 0.363410 1.3:-49275031 MS.gene54813:CDS
TCCAGCCCTCAGTCCTTGCC+AGG 0.368539 1.3:+49269256 None:intergenic
AGGGAAAATAAGAACCTTAC+AGG 0.372840 1.3:-49269593 MS.gene54813:CDS
TTTCAGTTGGGAGAGAGATT+CGG 0.379707 1.3:-49267340 MS.gene54813:intron
GTCATGGTCATCGACCTTCT+TGG 0.387600 1.3:-49274229 MS.gene54813:CDS
CGACCTTCTTGGACCCAGTC+TGG 0.390528 1.3:-49274218 MS.gene54813:CDS
ATGATTCTTCAAGGAGGAAC+TGG 0.404829 1.3:-49274295 MS.gene54813:CDS
ACCTCAACGAACGGCAATAG+TGG 0.405356 1.3:+49267911 None:intergenic
CCTCAACTCCAAACCATTTG+AGG 0.409202 1.3:+49274263 None:intergenic
GCTTTGAGCTCCTCACTATC+AGG 0.414666 1.3:-49266584 MS.gene54813:CDS
GAGATTCGGGAGAAATTATC+TGG 0.415618 1.3:-49267326 MS.gene54813:CDS
CTCAAATGGTTTGGAGTTGA+GGG 0.423483 1.3:-49274262 MS.gene54813:CDS
TCAGCTGTTAATATACAAAC+TGG 0.426788 1.3:-49274878 MS.gene54813:intron
GCAAGTGTCAACACCCATCT+TGG 0.433561 1.3:-49269557 MS.gene54813:CDS
ATAGGGATCTTCAGACTCAT+AGG 0.434481 1.3:-49269916 MS.gene54813:CDS
TTTATCATGGATCATGTGAT+TGG 0.443086 1.3:-49275062 None:intergenic
CCTCAAATGGTTTGGAGTTG+AGG 0.448526 1.3:-49274263 MS.gene54813:CDS
TTGCCGTTCGTTGAGGTTTG+AGG 0.451388 1.3:-49267905 MS.gene54813:CDS
ATATCGAGTTGAATTGTGTT+GGG 0.458129 1.3:+49266794 None:intergenic
ACAAAGTCGAAGGGATGATC+TGG 0.458546 1.3:-49269405 MS.gene54813:CDS
TTTCGCAAGGCCGAAGTCAA+TGG 0.458863 1.3:+49269943 None:intergenic
ATTGAATAAATCTTCCAGAC+TGG 0.465309 1.3:+49274204 None:intergenic
CATAAAGCACAAAAGATTCC+AGG 0.469567 1.3:-49267545 MS.gene54813:intron
AAACTGTATATGATTCTTCA+AGG 0.471640 1.3:-49274304 MS.gene54813:CDS
GTGGAAAGTTTAAACTTGGA+AGG 0.472881 1.3:-49275040 MS.gene54813:CDS
CTCCAAACCATTTGAGGTGG+GGG 0.473526 1.3:+49274269 None:intergenic
GGCAGGATGGCCACAAGTGG+GGG 0.486821 1.3:-49266711 MS.gene54813:CDS
ATAGTGAAGCTGTGGATGCC+TGG 0.493685 1.3:+49274333 None:intergenic
TCTTCCCTGGCAAGGACTGA+GGG 0.499281 1.3:-49269261 MS.gene54813:CDS
TTGTGGCCATCCTGCCAGAA+TGG 0.501550 1.3:+49266718 None:intergenic
CCCTGGCAAGGACTGAGGGC+TGG 0.502421 1.3:-49269257 MS.gene54813:CDS
GTCAACACCCATCTTGGAAT+CGG 0.505171 1.3:-49269551 MS.gene54813:intron
ATCATCATAAGTCCTATGTC+TGG 0.505727 1.3:+49267238 None:intergenic
CATATCGAGTTGAATTGTGT+TGG 0.505789 1.3:+49266793 None:intergenic
ATTGGTGGAAAGTTTAAACT+TGG 0.508093 1.3:-49275044 MS.gene54813:CDS
AGAGGAGGTGGCTGTTAAGC+TGG 0.511655 1.3:-49274855 MS.gene54813:intron
CAGGTGTATGCCATTGACTT+CGG 0.512497 1.3:-49269953 MS.gene54813:CDS
AGAATTTGCGCGGTTCCTCT+GGG 0.513807 1.3:+49267283 None:intergenic
CTGGCAGGATGGCCACAAGT+GGG 0.513957 1.3:-49266713 MS.gene54813:CDS
AGGGATGATCTGGAATCTCT+TGG 0.515030 1.3:-49269395 MS.gene54813:CDS
GAAGGTATCAACTCCAATAG+AGG 0.515426 1.3:-49269198 MS.gene54813:intron
GTCTTCCCTGGCAAGGACTG+AGG 0.516641 1.3:-49269262 MS.gene54813:CDS
AATTGTCTGGCTTTATGTCT+CGG 0.521040 1.3:+49273987 None:intergenic
ATCACTTACCGATTCCAAGA+TGG 0.522339 1.3:+49269543 None:intergenic
TCCACTATTGCCGTTCGTTG+AGG 0.522705 1.3:-49267912 MS.gene54813:CDS
CAGAGTAGTGGCAGCTCTAG+AGG 0.523385 1.3:-49267687 MS.gene54813:CDS
TCACTTACCGATTCCAAGAT+GGG 0.528445 1.3:+49269544 None:intergenic
AGGCCCATCAGAAAATTGTC+TGG 0.529417 1.3:+49273974 None:intergenic
AACTCCAAACCATTTGAGGT+GGG 0.533255 1.3:+49274267 None:intergenic
TGGCAGGATGGCCACAAGTG+GGG 0.534769 1.3:-49266712 MS.gene54813:CDS
CTCTATGTGCGCCAGATGGA+CGG 0.535759 1.3:+49266688 None:intergenic
CTTGATTTGCTTTACGTCCG+AGG 0.536022 1.3:+49273954 None:intergenic
TTGTAACGACAGCATGGCAC+TGG 0.538838 1.3:-49267599 MS.gene54813:intron
ATCATGGATCATGTGATTGG+TGG 0.540398 1.3:-49275059 MS.gene54813:CDS
TATACAAACTGGAGAGGAGG+TGG 0.543406 1.3:-49274867 MS.gene54813:CDS
TTGAATAAATCTTCCAGACT+GGG 0.547330 1.3:+49274205 None:intergenic
TAATGTATTTCTTAAGAGGA+AGG 0.552033 1.3:-49269364 MS.gene54813:intron
TAGTGGCAGCTCTAGAGGAC+GGG 0.553447 1.3:-49267682 MS.gene54813:intron
TCTGGCAGGATGGCCACAAG+TGG 0.554722 1.3:-49266714 MS.gene54813:CDS
GTAGTGGCAGCTCTAGAGGA+CGG 0.557752 1.3:-49267683 MS.gene54813:CDS
GTTGAATACATGCATTCAAG+AGG 0.558652 1.3:-49274019 MS.gene54813:CDS
TCATCAAAGCCAAATTCCTC+AGG 0.559975 1.3:-49266744 MS.gene54813:CDS
CAACTCCAAACCATTTGAGG+TGG 0.572196 1.3:+49274266 None:intergenic
TCCAGGAATCTGTGAAGACC+AGG 0.574379 1.3:-49274351 MS.gene54813:intron
CCTCAGGTGACCATTCTGGC+AGG 0.574601 1.3:-49266728 MS.gene54813:CDS
AGGTGACCATTCTGGCAGGA+TGG 0.577708 1.3:-49266724 MS.gene54813:CDS
TGGTGTGTTCACCGTGATGA+GGG 0.579905 1.3:+49267258 None:intergenic
CTCATAGGCACATACCGTAC+AGG 0.580718 1.3:-49269901 MS.gene54813:intron
AGACAGACTTACTAATTGAT+CGG 0.581760 1.3:+49274138 None:intergenic
CTTGGAAGGAAAATTGGGAG+TGG 0.582542 1.3:-49275026 MS.gene54813:CDS
CTTCCAGACTGGGTCCAAGA+AGG 0.585280 1.3:+49274215 None:intergenic
GATCACAAAACCAACCTGTA+CGG 0.586380 1.3:+49269887 None:intergenic
GAGATTACTTCCTGATAGTG+AGG 0.590566 1.3:+49266574 None:intergenic
CCAGCCCTCAGTCCTTGCCA+GGG 0.593406 1.3:+49269257 None:intergenic
ACTCCAAACCATTTGAGGTG+GGG 0.595250 1.3:+49274268 None:intergenic
TGAACTCTATGTGCGCCAGA+TGG 0.596034 1.3:+49266684 None:intergenic
ATTTGGCTTTGATGATACCA+TGG 0.597247 1.3:+49266752 None:intergenic
TGGTGGCAGAACAAAGTCGA+AGG 0.597531 1.3:-49269415 MS.gene54813:intron
GCTTGTTTCGTATCATACAC+AGG 0.597643 1.3:+49266660 None:intergenic
ACGGTGAACACACCAGACAT+AGG 0.599179 1.3:-49267250 MS.gene54813:CDS
GCTCGCAATGGGTCTATGAG+AGG 0.601655 1.3:-49266630 MS.gene54813:CDS
CCGGAAATTCACTTTGAAGT+CGG 0.603149 1.3:-49274173 MS.gene54813:CDS
CTGGTGTGTTCACCGTGATG+AGG 0.605762 1.3:+49267257 None:intergenic
AGGAGCTCAAAGCTCCTCAG+AGG 0.611695 1.3:+49266594 None:intergenic
ACATAGGACTTATGATGATG+TGG 0.615199 1.3:-49267234 MS.gene54813:intron
ACGACAGCATGGCACTGGCA+AGG 0.615258 1.3:-49267594 MS.gene54813:intron
TTGAAGTATCCACAGAGTAG+TGG 0.619683 1.3:-49267699 MS.gene54813:CDS
TTTGACTCATAGTGAAGCTG+TGG 0.620621 1.3:+49274325 None:intergenic
CGATAAAATCAGTGAGACGA+AGG 0.621170 1.3:-49269216 MS.gene54813:CDS
TGTCCTCAAACCTCAACGAA+CGG 0.628598 1.3:+49267902 None:intergenic
TGAGGGAGAGTACAATGTCA+TGG 0.631924 1.3:-49274245 MS.gene54813:CDS
AAATAAGAACCTTACAGGCA+CGG 0.632067 1.3:-49269588 MS.gene54813:CDS
GATGAGGGCTAGAATTTGCG+CGG 0.634849 1.3:+49267273 None:intergenic
TAATATACAAACTGGAGAGG+AGG 0.635465 1.3:-49274870 MS.gene54813:CDS
AAGCAGTTCAAGAAGAGCCA+TGG 0.646616 1.3:-49266769 MS.gene54813:CDS
CTAGAGCTGCCACTACTCTG+TGG 0.650464 1.3:+49267690 None:intergenic
AAGTATGATACAAACTCAGA+GGG 0.655305 1.3:+49267934 None:intergenic
TGTTAATATACAAACTGGAG+AGG 0.660638 1.3:-49274873 MS.gene54813:CDS
GAAGTATGATACAAACTCAG+AGG 0.660966 1.3:+49267933 None:intergenic
GGTGGCAGAACAAAGTCGAA+GGG 0.669079 1.3:-49269414 MS.gene54813:intron
TTGTTGAAGCATTCACCCAG+AGG 0.669158 1.3:-49267298 MS.gene54813:CDS
CCTGCCAGAATGGTCACCTG+AGG 0.679602 1.3:+49266728 None:intergenic
GCAAATTCTAGCCCTCATCA+CGG 0.684087 1.3:-49267269 MS.gene54813:CDS
AGCTCCTCAGAGGATCATCA+CGG 0.696325 1.3:+49266604 None:intergenic
GCCACCGTGATGATCCTCTG+AGG 0.725239 1.3:-49266608 MS.gene54813:CDS
CTGTATATGATTCTTCAAGG+AGG 0.728537 1.3:-49274301 MS.gene54813:CDS
TCCTCAGAGGATCATCACGG+TGG 0.751052 1.3:+49266607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.3 gene 49266578 49275078 49266578 ID=MS.gene54813
chr1.3 mRNA 49266578 49275078 49266578 ID=MS.gene54813.t1;Parent=MS.gene54813
chr1.3 exon 49275003 49275078 49275003 ID=MS.gene54813.t1.exon1;Parent=MS.gene54813.t1
chr1.3 CDS 49275003 49275078 49275003 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49274856 49274896 49274856 ID=MS.gene54813.t1.exon2;Parent=MS.gene54813.t1
chr1.3 CDS 49274856 49274896 49274856 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49274150 49274368 49274150 ID=MS.gene54813.t1.exon3;Parent=MS.gene54813.t1
chr1.3 CDS 49274150 49274368 49274150 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49273955 49274050 49273955 ID=MS.gene54813.t1.exon4;Parent=MS.gene54813.t1
chr1.3 CDS 49273955 49274050 49273955 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49269902 49269972 49269902 ID=MS.gene54813.t1.exon5;Parent=MS.gene54813.t1
chr1.3 CDS 49269902 49269972 49269902 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49269552 49269613 49269552 ID=MS.gene54813.t1.exon6;Parent=MS.gene54813.t1
chr1.3 CDS 49269552 49269613 49269552 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49269365 49269428 49269365 ID=MS.gene54813.t1.exon7;Parent=MS.gene54813.t1
chr1.3 CDS 49269365 49269428 49269365 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49269199 49269283 49269199 ID=MS.gene54813.t1.exon8;Parent=MS.gene54813.t1
chr1.3 CDS 49269199 49269283 49269199 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49267848 49267974 49267848 ID=MS.gene54813.t1.exon9;Parent=MS.gene54813.t1
chr1.3 CDS 49267848 49267974 49267848 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49267683 49267756 49267683 ID=MS.gene54813.t1.exon10;Parent=MS.gene54813.t1
chr1.3 CDS 49267683 49267756 49267683 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49267546 49267609 49267546 ID=MS.gene54813.t1.exon11;Parent=MS.gene54813.t1
chr1.3 CDS 49267546 49267609 49267546 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49267235 49267356 49267235 ID=MS.gene54813.t1.exon12;Parent=MS.gene54813.t1
chr1.3 CDS 49267235 49267356 49267235 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
chr1.3 exon 49266578 49266823 49266578 ID=MS.gene54813.t1.exon13;Parent=MS.gene54813.t1
chr1.3 CDS 49266578 49266823 49266578 ID=cds.MS.gene54813.t1;Parent=MS.gene54813.t1
Gene Sequence

>MS.gene54813

ATGGATCATGTGATTGGTGGAAAGTTTAAACTTGGAAGGAAAATTGGGAGTGGTTCTTTTGGAGAGCTCTATTTAGGTTTGTTCATCTTTATTCCCTTTTTTTTGTTTTTAATTCATTTATTTGGGTACATTGTATTGTTTTGTTAATATTAACATGTTCATTCTATTGTTTCTTATATCAGCTGTTAATATACAAACTGGAGAGGAGGTGGCTGTTAAGCTGGTATGTGAAGTTCTCTTCAATCATTTGACTCATTTTTATTTTTTATTGTTGAATTCTTTTATGAATTTCTAGAATTTGGTTGATTTTTTTTTTCATGTTGGTTCTAGATGTATTATTAGGATATTATCTGAACTATTTAGGGTCAATGATGATCCATGAAATTGAGCATGCTTGCGATATCATTTATCGAAGGAAATGGCATGAGATATGTTTTAAATGTTAACTTTTGGTCAATAGGTGGATGATTGGTTCTTGAAATGAGATGTGAAATGGCACATTATGTTTATCCTCACAATTCTGTTTTGCAGCTGCTAGATGCTCACTTCGTATCATATTTTTACAATACATTTTCTGTTGCATTGTTGATCACGTTTTGCTAGTTTGTGAAAGATTTTTTTTTTGTTATCAGTTAGTGGTGGCACTTCATGTGACACACTATGTTTTTCCAATTTCCAATTGTCTCACGGTTCTGTCAAAAATATTCCAGGAATCTGTGAAGACCAGGCATCCACAGCTTCACTATGAGTCAAAACTGTATATGATTCTTCAAGGAGGAACTGGAATCCCCCACCTCAAATGGTTTGGAGTTGAGGGAGAGTACAATGTCATGGTCATCGACCTTCTTGGACCCAGTCTGGAAGATTTATTCAATTATTGTAACCGGAAATTCACTTTGAAGTCGGTGTTGATGCTTGCCGATCAATTAGTAAGTCTGTCTGTCAAGTTGGCGAAAGTTATTTAATATGGTTCTTTATATAATGGTACTAATAGCTTATTTGCCTCACACTTTTGTTTGTTTTTTCAGATTAACAGAGTTGAATACATGCATTCAAGAGGCTTTCTTCACCGAGACATAAAGCCAGACAATTTTCTGATGGGCCTCGGACGTAAAGCAAATCAAGTATGCGATTATTGTCTGTTGAAGATTTCTTGTTTTTTTTTTTTTTTTTTTTAATTCCTTTGTCTATTGTGGGTTCAATATGCCTTTTGAAAAGTTTGATAGAGGAGTATGGGAGAAGCCTAAATTATCTATATATGATTATTATTGACCTAGAAAAGAATTATGATAGGGTGTCTATGATAGATATCGGTTCTGAACAGAAACAGAATTACACAATACTAACAATTTATTGATAGAAACTCTGGTAAATGATTCACTACCAGAATATTACAGACACACAGTACAATAAATATACAAAATAACATAACACAGACACACCAAGATTCGAGAGCTTGGCCTCTCCCCTTCCCAAATGTTCGAGAGTTGGGTCTCTCCCAATAACCACTCAATAACTTCCCGATATCCCTCTTATGTCTGACCCTTCTCTTATTTATCCTTAAAACATAACTGACTCAATTAATAATTAATTAATAAAACATAACTGACACAACACAAATAAGACAGAACCTAACTGTCCTAAAATAACTGCTAGTCTAACAATACTCCCCCCCAAAAGATTCACCTTGTCCTCAAGGTGAAGAATTACACACGTGTCCCCCAGGATCCCAGAACTTGAATTCTGTCATCCCTTCTTCGGCCTTTTCTTCTTTATAATCTTCTTCAGCCATTATCATGATCCGTAACTGTTTGTTGGGACATCTATGACCTGGACTAAATTTCTCATCACACCGGAAACATAATCCTTTTCCCCTTTTATCTTGAATCTCTGCATCAGTTAGTTTCTTAAACATTTGTCCTTTAATCGCCTCAGAAGTCTTTGAATTATCGCTCTCTCCCATGGAATTTCCGACCCCGGATTGGTTTGTTTGAACATGACTTTTGTTTTCTGACTCCCAAATTGCAGTTTTGGACTTATTGGAAGTTCTCATTGTTCTTCCAATTGAACCCGTTTCATTACTCCCTTTTGTTAATAACATATTTTTCTCGTCAATGCGTTGGGCATAATCCATCAATTCGGGTAACGACCCGACAGGATGAAGTTTCAATTCAGCCTTAATCACATCCTGCAATCCACTAAGGAAAATACCTTTCAGATATTCTGGTTCGGCACTTCGTAGTGGGCCTGCATATAATTCAAATTGTTCACGGTATAATTCGACGGAACCAATTTGTTTGAGACTCATCAGAAGTTCATATGGGCTTTGAAGCATTGAAGGTTGGAACCGTTTTAATAAAGCTTCCTTAAATGCACTCCATGTTTGGTTTGGGGAACAAAATTCCCACCATTGATACCAAGTAAGGGCCTTGCCTTCCATTGCTACCATGGCTGGTTGCAGTTTTTCTTCATCCAATACACCTTTTAAATCGAAATAACGTTCCACCCGATTTATCCAACCAAACGCATCGTCACCGTCGAAAATTGGAATTGACAGTTTTCGCCAACGTTCTTCTGGTGCCCGCTGTTCTGTGTGATACCCATTGTTGGAATCGGCCTGATGATGTTCCTGGCTTGGTGAAGAATGCGTCAGGGATAGGCCGCGAACGGTTTCTTGCAATTCATTGATGCGTCGCTGGGCTTCAATTTTCTCTGTTTCTAACTGAAGACGCAAATCTTGAACTTCATCACGAGTTTTCATGTAATCATGGTATGCTCTTTCCAAAAATTCTAAACGTGCCTCCATTTCTCCCCGCGTAGATACCATGAAGCAGTCCCGGAAGGTAACTGCTCTGATACCAGTGATAGATATCGGTTCTGAACAGAAACAGAATTACACAATACTAACAATTTATTGATAGAAACTCTGGTAAATGATTCACTACCAGAATATTACAGACACACAGTACAATAAATATACAAAATAACATAACACAGACACACCAAGATTCGAGAGCTTGGCCTCTCCCCTTCCCAAATGTTCGAGAGTTGGGTCTCTCCCAATAACCACTCAATAACTTCCCGATATCCCTCTTATGTCTGACCCTTCTCTTATTTATCCTTAAAACATAACTGACTCAATTAATAATTAATTAATAAAACATAACTGACACAACACAAATAAGACAGAACCTAACTGTCCTAAAATAACTGCTAAAATAACTGCTAGTCTAACAGTCTATAGACAAGTATAACACATGGTAGGGCCCATTTTAAGCTATTATGTTTTTTCTTTGGTATTAAATGTTCTTACTAGCATATCCAGGAGGTAACACTGTCATATATTTTCTTGTGTGGGTTGTATGGTCATAGTTGAAGAGTCTAAATAGGAATAAATGGTAAACTAGAGTTGTGAAGAGAAGCTTAAAATCACATGATTTATGATTAAATATAAGAAAATCTTGGGAGTACATTTGTTGAGTCCCACATTGGATATGGTATGACCTGAATATTAAGAGGGGAATATTGGAGGTCATATTAATGCCACAACACAAGGTGCACAGTTTCGGCCCCTTGAATCTATCATACCAAGCGACGAAGAAATGGAGAAAGATATAAATCGTAGGATTCAAGTGAGTTGGATGAAATGGAGAAATATTTCAGTTATTTGTGTTTGAAAGGTACCTCTCAAAAGTTTGAAGGAAATAAAGTTCCAACAAGAGAAGTCAAGTGTTTTAGAGATGAGAATTTTGCAGGGGATGAGTGGACATACAAGAAAAGATATGATTGTGCAATGGAATAACACCTGTCAGGACTACGAATTTACAACCCAAGAGAGAGTAGGAAAAACACTTATTACCGAAAAAATGATAGAATCTTGTCTAACATGTGAGGAGTCTAATAGAAACCCTGGTAAAAAAAGTTGATGTTAGAAACCTTGAAAAGAACTGAGCAACAGAAAAGATGATTTTTATTGATTGAATTGAACTGAGTATATAGTTAGTGATTCAATAATAGCATCTACAGTTTCTGTATTATATTGATATTGATAGCGGCACTTCTACTTGTATCTGTTAGACATGTTAGATTCCGTTACATAGTGCAATCAAGCAACTGCAAGCTAGTCAGGTTCTTTAACTAACATTTTAGTTGCAATCAAGCAGCTGCAAGTTAGTTTTTGACAGAATGCAACGGCGTCATTTGATTCGTGGTTTTAATGGTTGTGTAGTTTGGATGATTTTGGTTTTTATTTTCCTGTCTTTGATATAAATAAAGAGTTACCCATTATAATGAGTCTTCGGCATTCAATAACATCAAAATACAAGCTTGGTTGAGTAACATTTGAATTGGCAGTGTCCTGTTTCCATGTTTGTCCTTGAGTTTTTCAAGAGTACTCTCACTTCGTCCTGGACCTTTCCTTTAAAATCCTAAGTTCACCAGTCATTTTACTTATTTTCATATCACGTCTATTGGTTTACTTCATAATCTATCATAGTAATATTTACTTTTTGGGAGGGTGTGTTTCTCCTTTAGTTCAATGACTTACGCTGTAGATACTGTTGCCGTTCATAGCATAGATCCCTGCACGACAAATTTAGGAAATAAATGTACTGCCCTTGAGTATAATGTTGAGTTGGTCTTACTTGAGAAAATGTCTGCTTTGACATGTGCACACATTTTGGCATGCAAGTGACTTTATGTTGGTATCAGTTTATTGATTTCATCGTGGCATACCAACCATACAGTGCTAATTATAACATGATTAACTTCTAGTGAGACTAGATTACCTAGCGTTAACACGATAAAATGAAGCCTAAACTTAACCGTTTGTATCCTTTAAAAGACTTCATTTTGTAGTCTCCTTGTTGGTCAAAGCAATTGGTTTCTTTAAGGGTGGGAATTTCTTAATCGTTGGATATGCTAAGTTTGTACAGCACGTTGAGTTAATATTCTTTCCATTTTTTATTTTATTTTATGGATGTTATAGGAGTGTTTCTGTTTGTCTATCTTTTATTTCAATTTTTTATTTATTTTAATAAATTTTTATTCTCTTGTCCAAAACTAAATATAATCGACTGATACATCACACTCTGTATCTCATTTCAGGTGTATGCCATTGACTTCGGCCTTGCGAAAAAATATAGGGATCTTCAGACTCATAGGCACATACCGTACAGGTTGGTTTTGTGATCATAATTTTAGGAGGGAGCAATATTAGGTTAAGCTAAACAATTAGGCAAACTAAACCTTATATTGCTTAATAATTGCTTAGTTTTTTTTAATTTTTTTTTCTACGCATTTGCATCCCTTTAACAGGCTGTTATGCCATTTTTTTTACTGGAAATCACTACCTAAATGTAGATAATATTTTAGTATACTTTAACTAAAACAATATCAAACTTGGTCCCACAAATTCCCATTGATCTCAATATATTTTGTAAGTGATATTTTTCTTCTTTGCTTAGGGAAAATAAGAACCTTACAGGCACGGCACGCTATGCAAGTGTCAACACCCATCTTGGAATCGGTAAGTGATATTTTATTCTGGTTTGTTACTTTCTCGGTTAGGACTATTCAGTTCAAAATGAATTATTACATTATTAGGACTGAATGTGTTTTCTATATACTAATTGTGTGATTGTGGTGGCAGAACAAAGTCGAAGGGATGATCTGGAATCTCTTGGTTATGTGCTAATGTATTTCTTAAGAGGAAGGTTAGTTAATTGACAAACGCATCTACAACTTTATAGTTTATTCACACTATCAACTTTGTTTCATACTTTTTCCTTTTTCAGTCTTCCCTGGCAAGGACTGAGGGCTGGAAACAAAAAGCAAAAATACGATAAAATCAGTGAGACGAAGGTATCAACTCCAATAGAGGTAATTATGACTTGACTTCCTGCATCTGTCAGTTTTTTTTTTTTTTTTTTATATTTTTTTAATGAAATTAAATTAATACCAGTTATTGAGCACTATTGCATGTTTCTTGTTTATTAGAAATTGGAATTAACTAAACACTCTCAAGTTGATGTTTGCTTTTTTAATTATTTACCTATATTTCCTGCATTCAACTGTTGGCACTTAGGGTGTGTTTGGATGGAGTGTTTAGGAGGGGAGAGTTTACTTTTCATTTTTAAATTACGGACACTATGAAATGTTTTTTTTAAAAAGTTAACATATTAACATGATAAACGATCATGTAATATTTCAATCACACTCAGTGGCGTAGCCAGAAACTTTGAATAGTGGGGGCACTATATATAAATTTGTAGCATAGCCATCATCTTAAATAATTTGGATCTTAAACAATATAGACGTCGATGTACAATACTAACTTAGAGGTCGATACCAATTTAGAGTTGTCATTTTGGTCTCTAAATATATGGCGTGTTATCACTAAAATATTTGAATTTTATCAAAACTATGAGCATATCTTCAAATGTTTCATTTGTTATTAATTGTATAGACCAAATTGATTAGTGAAAAATATATATAAAGATTAACTTAGCTAACTAAAGACACATTTAAAAATTAAAATGACTAATAAAAACTAAGGTTTGTTTTTGGTAATTTTAATAGAAATTATTAATAACCCATTTTAATTCAAAACCTTCAAGTTTTTCCATAATATAACATTACATAATCATCATACATTAATTATTAGGAAAATGAAACCAACACAACAGAAAGTGTTGAGAGATGCAGCCAAGCGTTTTTGTTTAAAGGCTCCATAACATCTTTAGGAATGCTTGGTAAAACTTTTTACTAAATATTATACATAAATATATAATATATGTATTAGTGTGTATATATGTAATGCATGAAGCAACTTTCAAAAATTAGTGGGGGCACAAGCCCACAGATAGCTTAACGTGGCTCCGCCACTGATCACACTTAATTTATTTTAGAAAAACATGTTCTATAGTCCGTAATTTGAAAAAGAAAAGTAAACCCTCCCCTCCCTTCCCCCTCCTAAACCCTCAATCCAAACACACCCTAAAATGTTATTTTCATAAAACTTTCCTCTTCATTTCCCTTCTGTCTCATGTAAAATCTTAATTTTATCGCTCTTGTTGATAGAATTACGTGCTTTTATAAAAATAGGTGCTCTGTAAATCATATCCCTCTGAGTTTGTATCATACTTCCACTATTGCCGTTCGTTGAGGTTTGAGGACAAACCAGATTATTCATATTTAAAGAGACTTTTCCGAGACCTTTTTATTCGAGAAGGTATGTTACCCAAAATAATGTGGAAAATGCATTCAGTATATTTTGCTGGAAATTCGGTGGTTGTGAATTGACGTGGGCATTGATCATGCAGGCTATCAATTTGACTATGTTTTTGACTGGACTGTATTGAAGTATCCACAGAGTAGTGGCAGCTCTAGAGGACGGGTTAGTATTTTATCTATAGATCTTGTTGTTTTACATTTTTGTTGGTCGCTTACTGTGGTTGTTGTAACGACAGCATGGCACTGGCAAGGCAGCTATGCATGCAGCTACACCAGTACATAAAGCACAAAAGATTCCAGGTCTGTTGTCTTTCAGCTGAATAAGAATGAAATTCGGTATCCTGCTAATATGTGCTGTGACAGAAAATAAATGTTTGAACTGAAAGCATTCAACTGAAGTTATCTTTTTCGTTGTTCTCATCAATAGTAAAAATATTGTACTGTTTTCAAAGTCTGTTGTATAATATTTGTTCATGCTATTTGTTTCAGTTGGGAGAGAGATTCGGGAGAAATTATCTGGTGCTGTTGTTGAAGCATTCACCCAGAGGAACCGCGCAAATTCTAGCCCTCATCACGGTGAACACACCAGACATAGGACTTATGATGATGTGGTAATACTCTTTCACAACAATAAGAATTTTTAAATCATCATATGAAGTTTTCAAATGAGAATGTTGTTTCCTGTTTGGTTTGACAAGACGTTTTCTAGTTCCATTTCATGTTTTTTCACATAATTACAAAAATGTCCCTTTTTTTTACCTTGTTTACCATTTGTAAATATTTGTACTTGAAATTATAAGCACGATAAAATATTGCTTTCACCGTTTTATTTACAAATCTAAAAATTAAGTAATGAAGTGAAAGAAAACAAGTCTGTTTTTAATAATTTACAATGAGAACAGAAAGTGTTTTGTCAAATCAAACAAACGTATTCTCTTAATATTTAGTCAAGTCTTGAGTGTAACTATGCATTTGTTTATTGAGTTATTATTCTTATGTTTAACGTTATCTTATATTAACAGCACTATGCCCAACACAATTCAACTCGATATGGAAGCAGTTCAAGAAGAGCCATGGTATCATCAAAGCCAAATTCCTCAGGTGACCATTCTGGCAGGATGGCCACAAGTGGGGGCCGTCCATCTGGCGCACATAGAGTTCAACCTGTGTATGATACGAAACAAGCAACTTTTGCTCGCAATGGGTCTATGAGAGGCCACCGTGATGATCCTCTGAGGAGCTTTGAGCTCCTCACTATCAGGAAGTAA
Protein sequence

>MS.gene54813.t1

MDHVIGGKFKLGRKIGSGSFGELYLAVNIQTGEEVAVKLESVKTRHPQLHYESKLYMILQGGTGIPHLKWFGVEGEYNVMVIDLLGPSLEDLFNYCNRKFTLKSVLMLADQLINRVEYMHSRGFLHRDIKPDNFLMGLGRKANQVYAIDFGLAKKYRDLQTHRHIPYRENKNLTGTARYASVNTHLGIEQSRRDDLESLGYVLMYFLRGSLPWQGLRAGNKKQKYDKISETKVSTPIEVLCKSYPSEFVSYFHYCRSLRFEDKPDYSYLKRLFRDLFIREGYQFDYVFDWTVLKYPQSSGSSRGRHGTGKAAMHAATPVHKAQKIPVGREIREKLSGAVVEAFTQRNRANSSPHHGEHTRHRTYDDVHYAQHNSTRYGSSSRRAMVSSKPNSSGDHSGRMATSGGRPSGAHRVQPVYDTKQATFARNGSMRGHRDDPLRSFELLTIRK