Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54995.t1 | XP_003624429.1 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 8.80E-31 | 143.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54995.t1 | B9RH17 | 59.4 | 69 | 28 | 0 | 115 | 183 | 119 | 187 | 2.4e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54995.t1 | G7KW47 | 98.6 | 72 | 1 | 0 | 115 | 186 | 115 | 186 | 6.3e-31 | 143.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene025030 | MS.gene54995 | PPI |
| MS.gene54995 | MS.gene63947 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54995.t1 | MTR_7g083120 | 96.774 | 186 | 6 | 0 | 1 | 186 | 1 | 186 | 1.45e-133 | 371 |
| MS.gene54995.t1 | MTR_7g083120 | 95.726 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 3.03e-76 | 227 |
| MS.gene54995.t1 | MTR_4g118810 | 29.655 | 145 | 88 | 3 | 33 | 166 | 36 | 177 | 5.29e-16 | 72.8 |
| MS.gene54995.t1 | MTR_0004s0210 | 29.927 | 137 | 88 | 1 | 33 | 169 | 11 | 139 | 1.59e-15 | 70.9 |
| MS.gene54995.t1 | MTR_4g081880 | 32.099 | 162 | 105 | 3 | 11 | 169 | 10 | 169 | 1.85e-14 | 68.6 |
| MS.gene54995.t1 | MTR_7g108130 | 30.496 | 141 | 92 | 3 | 33 | 169 | 32 | 170 | 6.94e-14 | 67.0 |
| MS.gene54995.t1 | MTR_4g118800 | 36.000 | 125 | 79 | 1 | 33 | 157 | 31 | 154 | 9.21e-13 | 63.9 |
| MS.gene54995.t1 | MTR_2g020060 | 27.778 | 162 | 104 | 2 | 33 | 184 | 26 | 184 | 2.74e-12 | 62.8 |
| MS.gene54995.t1 | MTR_7g011090 | 30.435 | 161 | 107 | 3 | 11 | 169 | 11 | 168 | 1.23e-11 | 60.8 |
| MS.gene54995.t1 | MTR_5g076480 | 24.242 | 165 | 118 | 3 | 22 | 186 | 29 | 186 | 3.80e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54995.t1 | AT4G25040 | 36.424 | 151 | 92 | 1 | 31 | 181 | 18 | 164 | 3.48e-21 | 85.9 |
| MS.gene54995.t1 | AT4G03540 | 34.959 | 123 | 72 | 1 | 47 | 169 | 26 | 140 | 6.31e-18 | 77.0 |
| MS.gene54995.t1 | AT4G15630 | 26.984 | 189 | 119 | 4 | 6 | 184 | 10 | 189 | 1.12e-15 | 72.0 |
| MS.gene54995.t1 | AT4G15620 | 30.435 | 161 | 100 | 3 | 34 | 184 | 31 | 189 | 3.41e-15 | 70.5 |
| MS.gene54995.t1 | AT1G03700 | 32.143 | 112 | 68 | 1 | 45 | 156 | 24 | 127 | 3.23e-14 | 67.4 |
| MS.gene54995.t1 | AT4G15610 | 28.105 | 153 | 105 | 2 | 10 | 157 | 9 | 161 | 4.20e-12 | 62.4 |
| MS.gene54995.t1 | AT5G15290 | 30.216 | 139 | 90 | 3 | 33 | 167 | 31 | 166 | 7.69e-12 | 61.6 |
| MS.gene54995.t1 | AT3G06390 | 25.455 | 165 | 108 | 5 | 5 | 157 | 9 | 170 | 4.19e-11 | 59.7 |
Find 29 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGCCTTCTTTGCCAATT+TGG | 0.186565 | 7.2:+24224866 | MS.gene54995:CDS |
| TTGGCCTTCTTTGCCAATTT+GGG | 0.251825 | 7.2:+24224867 | MS.gene54995:CDS |
| AAAGTTCTGCAAAACCAATT+TGG | 0.258540 | 7.2:+24224824 | MS.gene54995:CDS |
| TAAAATTGTGAGTCCCAAAT+TGG | 0.269455 | 7.2:-24224880 | None:intergenic |
| TTCCATCCTGAAGGAATCTT+TGG | 0.318409 | 7.2:-24223446 | None:intergenic |
| AAGCAAAAGTGAAACCAAAT+TGG | 0.326060 | 7.2:-24224838 | None:intergenic |
| GGATGAGGTTGTTGAAGATT+TGG | 0.339479 | 7.2:-24223470 | None:intergenic |
| ACATCATAGGACTTTCTTCA+TGG | 0.382860 | 7.2:+24223501 | MS.gene54995:CDS |
| CTGTTATGCTCTTCTCTATC+CGG | 0.383937 | 7.2:+24223599 | MS.gene54995:CDS |
| GATGAGGTTGTTGAAGATTT+GGG | 0.392076 | 7.2:-24223469 | None:intergenic |
| TAATTAAGAGGTGAACTTGT+AGG | 0.396788 | 7.2:-24224907 | None:intergenic |
| GTTGGATACGTGGGACAGTA+TGG | 0.412618 | 7.2:+24224759 | MS.gene54995:CDS |
| CTGATGCCAAAGATTCCTTC+AGG | 0.434784 | 7.2:+24223440 | MS.gene54995:CDS |
| TCAAAACATCCTCAGAATCT+TGG | 0.440400 | 7.2:+24223525 | MS.gene54995:CDS |
| GAGAGGAGAAAATAATTAAG+AGG | 0.459921 | 7.2:-24224919 | None:intergenic |
| ATGACGGTGTTGCTGATAGC+TGG | 0.468432 | 7.2:+24224717 | MS.gene54995:CDS |
| ATGGAGAGAAACACGTTGGA+TGG | 0.514689 | 7.2:+24224778 | MS.gene54995:CDS |
| GAGCATAACAGATTGGTTGT+TGG | 0.526003 | 7.2:-24223587 | None:intergenic |
| GAGTCCCAAATTGGCAAAGA+AGG | 0.541836 | 7.2:-24224871 | None:intergenic |
| TAGAGAAGAGCATAACAGAT+TGG | 0.552990 | 7.2:-24223594 | None:intergenic |
| AGATTTGGGTTCCATCCTGA+AGG | 0.555523 | 7.2:-24223455 | None:intergenic |
| AGCCACAGCCGTTGGATACG+TGG | 0.562855 | 7.2:+24224749 | MS.gene54995:CDS |
| TGTTCAGCAGCCACAGCCGT+TGG | 0.589627 | 7.2:+24224741 | MS.gene54995:CDS |
| TCCCACGTATCCAACGGCTG+TGG | 0.593429 | 7.2:-24224751 | None:intergenic |
| GCCACAGCCGTTGGATACGT+GGG | 0.599202 | 7.2:+24224750 | MS.gene54995:CDS |
| TGCCAAAGATTCCTTCAGGA+TGG | 0.603471 | 7.2:+24223444 | MS.gene54995:CDS |
| GAACAATGACCAAGATTCTG+AGG | 0.616784 | 7.2:-24223534 | None:intergenic |
| ATACTGTCCCACGTATCCAA+CGG | 0.624247 | 7.2:-24224757 | None:intergenic |
| CAGTATGGAGAGAAACACGT+TGG | 0.708078 | 7.2:+24224774 | MS.gene54995:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGACATTATATTTTTTAT+AGG | + | chr7.2:24224355-24224374 | MS.gene54995:intron | 10.0% |
| !!! | ATGACATTATATTTTTTATA+GGG | + | chr7.2:24224356-24224375 | MS.gene54995:intron | 10.0% |
| !! | TCATATGTATAAACAAAAAA+GGG | + | chr7.2:24223693-24223712 | MS.gene54995:intron | 15.0% |
| !! | TTCAATATAATTATACATGT+CGG | - | chr7.2:24224490-24224509 | None:intergenic | 15.0% |
| !! | TTCATATGTATAAACAAAAA+AGG | + | chr7.2:24223692-24223711 | MS.gene54995:intron | 15.0% |
| !! | TTTGTTTATACATATGAAAA+TGG | - | chr7.2:24223690-24223709 | None:intergenic | 15.0% |
| !!! | TGACATTATATTTTTTATAG+GGG | + | chr7.2:24224357-24224376 | MS.gene54995:intron | 15.0% |
| !!! | TGATTTTCTTAAATTATTAG+CGG | + | chr7.2:24224515-24224534 | MS.gene54995:intron | 15.0% |
| !! | AAACAATGACATGTATAATT+TGG | - | chr7.2:24224303-24224322 | None:intergenic | 20.0% |
| !! | ACTTGACTAAAACATTAAAT+GGG | - | chr7.2:24224247-24224266 | None:intergenic | 20.0% |
| !! | ATACATATGAAAATGGAAAA+TGG | - | chr7.2:24223683-24223702 | None:intergenic | 20.0% |
| !! | TACATATGAAAATGGAAAAT+GGG | - | chr7.2:24223682-24223701 | None:intergenic | 20.0% |
| !!! | CTATTATTTCTTCCTATTTT+TGG | + | chr7.2:24223872-24223891 | MS.gene54995:CDS | 20.0% |
| !!! | TCTGTATTTATTTTTGTCTT+TGG | + | chr7.2:24223747-24223766 | MS.gene54995:intron | 20.0% |
| ! | AATAAAAATGAGCTTCAAAC+CGG | - | chr7.2:24223621-24223640 | None:intergenic | 25.0% |
| ! | ATACATGTCATTGTTTATCA+TGG | + | chr7.2:24224307-24224326 | MS.gene54995:intron | 25.0% |
| ! | GACTTGACTAAAACATTAAA+TGG | - | chr7.2:24224248-24224267 | None:intergenic | 25.0% |
| ! | TCTTTCTAATCCACAAAATT+AGG | + | chr7.2:24224625-24224644 | MS.gene54995:intron | 25.0% |
| ! | TTACCAAATCAACCAAAAAT+AGG | - | chr7.2:24223887-24223906 | None:intergenic | 25.0% |
| ! | TTAGAAAGAAAGGTAAATGA+TGG | - | chr7.2:24224614-24224633 | None:intergenic | 25.0% |
| !! | AAATAATAGTCTTTTCGTTG+AGG | - | chr7.2:24223861-24223880 | None:intergenic | 25.0% |
| !! | GGAATATGATTGATAATATG+TGG | + | chr7.2:24224328-24224347 | MS.gene54995:intron | 25.0% |
| !! | TGATAATCAATCTTTTGATG+AGG | + | chr7.2:24223826-24223845 | MS.gene54995:CDS | 25.0% |
| !!! | AAAATTGGTGAATTTTGATC+AGG | + | chr7.2:24224692-24224711 | MS.gene54995:intron | 25.0% |
| !!! | AAGCCAATAACATTTTAACA+TGG | - | chr7.2:24223981-24224000 | None:intergenic | 25.0% |
| !!! | ATTTTGTGGATTAGAAAGAA+AGG | - | chr7.2:24224624-24224643 | None:intergenic | 25.0% |
| !!! | CATGTTTGTTATGAGAAAAT+TGG | + | chr7.2:24224677-24224696 | MS.gene54995:intron | 25.0% |
| !!! | TTACTAGTGTCAATTTTAGT+GGG | + | chr7.2:24224160-24224179 | MS.gene54995:intron | 25.0% |
| !!! | TTCACTTTTGCTTTCATATT+TGG | + | chr7.2:24224848-24224867 | MS.gene54995:CDS | 25.0% |
| AAAGTTCTGCAAAACCAATT+TGG | + | chr7.2:24224824-24224843 | MS.gene54995:CDS | 30.0% | |
| AAGCAAAAGTGAAACCAAAT+TGG | - | chr7.2:24224841-24224860 | None:intergenic | 30.0% | |
| GGTCCATGTTAAAATGTTAT+TGG | + | chr7.2:24223975-24223994 | MS.gene54995:intron | 30.0% | |
| TAAAATTGTGAGTCCCAAAT+TGG | - | chr7.2:24224883-24224902 | None:intergenic | 30.0% | |
| TCTTAAATTATTAGCGGTGT+CGG | + | chr7.2:24224521-24224540 | MS.gene54995:intron | 30.0% | |
| ! | CTTACTAGTGTCAATTTTAG+TGG | + | chr7.2:24224159-24224178 | MS.gene54995:intron | 30.0% |
| !!! | AATAGTTTTGAGTTAGACCT+TGG | + | chr7.2:24224104-24224123 | MS.gene54995:intron | 30.0% |
| !!! | AGATTTTTTGTTGCTGCAAA+TGG | + | chr7.2:24223774-24223793 | MS.gene54995:intron | 30.0% |
| !!! | CTTCCTATTTTTGGTTGATT+TGG | + | chr7.2:24223881-24223900 | MS.gene54995:CDS | 30.0% |
| ACATCATAGGACTTTCTTCA+TGG | + | chr7.2:24223501-24223520 | MS.gene54995:CDS | 35.0% | |
| CATCCTCACAAAAACATCAT+AGG | + | chr7.2:24223488-24223507 | MS.gene54995:CDS | 35.0% | |
| GACAGTATCTATGTTATGTC+TGG | + | chr7.2:24224547-24224566 | MS.gene54995:intron | 35.0% | |
| GATGAGGTTGTTGAAGATTT+GGG | - | chr7.2:24223472-24223491 | None:intergenic | 35.0% | |
| TATCCGTATTCATGCTTCAT+AGG | + | chr7.2:24224576-24224595 | MS.gene54995:intron | 35.0% | |
| TCAAAACATCCTCAGAATCT+TGG | + | chr7.2:24223525-24223544 | MS.gene54995:CDS | 35.0% | |
| TGACCTATGAAGCATGAATA+CGG | - | chr7.2:24224582-24224601 | None:intergenic | 35.0% | |
| ! | CTTATTCGCTCCTAATTTTG+TGG | - | chr7.2:24224638-24224657 | None:intergenic | 35.0% |
| ! | TAGAGAAGAGCATAACAGAT+TGG | - | chr7.2:24223597-24223616 | None:intergenic | 35.0% |
| ! | TTCACAGATGTCTTTTACTC+TGG | + | chr7.2:24224459-24224478 | MS.gene54995:intron | 35.0% |
| ! | TTGGCTTAGACATGTTTTGT+CGG | + | chr7.2:24223994-24224013 | MS.gene54995:intron | 35.0% |
| ! | TTTTGCAGAACTTTTGCACA+TGG | - | chr7.2:24224818-24224837 | None:intergenic | 35.0% |
| !! | AGTCCTATGATGTTTTTGTG+AGG | - | chr7.2:24223494-24223513 | None:intergenic | 35.0% |
| !! | ATGATGTTTTTGTGAGGATG+AGG | - | chr7.2:24223488-24223507 | None:intergenic | 35.0% |
| !! | GAATTTTGATCAGGTGATGA+CGG | + | chr7.2:24224701-24224720 | MS.gene54995:intron | 35.0% |
| !! | TGAAAATGGAAAATGGGTTG+TGG | - | chr7.2:24223676-24223695 | None:intergenic | 35.0% |
| !!! | GTCAATTTTAGTGGGTTAGT+CGG | + | chr7.2:24224168-24224187 | MS.gene54995:intron | 35.0% |
| ATTGAAGTCGAGACTTTGAG+AGG | - | chr7.2:24224138-24224157 | None:intergenic | 40.0% | |
| CTGTTATGCTCTTCTCTATC+CGG | + | chr7.2:24223599-24223618 | MS.gene54995:CDS | 40.0% | |
| GAACAATGACCAAGATTCTG+AGG | - | chr7.2:24223537-24223556 | None:intergenic | 40.0% | |
| GAGTTGTAACCTACTTAGGT+TGG | + | chr7.2:24224073-24224092 | MS.gene54995:intron | 40.0% | |
| GGATGAGGTTGTTGAAGATT+TGG | - | chr7.2:24223473-24223492 | None:intergenic | 40.0% | |
| TCAACCAAACCAACCTAAGT+AGG | - | chr7.2:24224085-24224104 | None:intergenic | 40.0% | |
| TGGTTAATTACCTCAGCACT+AGG | - | chr7.2:24224283-24224302 | None:intergenic | 40.0% | |
| TGTCTGAAAACAGAAATCGC+AGG | + | chr7.2:24223954-24223973 | MS.gene54995:intron | 40.0% | |
| TTCCATCCTGAAGGAATCTT+TGG | - | chr7.2:24223449-24223468 | None:intergenic | 40.0% | |
| ! | GAGCATAACAGATTGGTTGT+TGG | - | chr7.2:24223590-24223609 | None:intergenic | 40.0% |
| ! | TTGGCCTTCTTTGCCAATTT+GGG | + | chr7.2:24224867-24224886 | MS.gene54995:CDS | 40.0% |
| ! | TTTGAGAGGAGTATACTCCA+AGG | - | chr7.2:24224124-24224143 | None:intergenic | 40.0% |
| ! | TTTGGCCTTCTTTGCCAATT+TGG | + | chr7.2:24224866-24224885 | MS.gene54995:CDS | 40.0% |
| ! | TTTTGTTGCTGCAAATGGTG+TGG | + | chr7.2:24223779-24223798 | MS.gene54995:CDS | 40.0% |
| !! | GTAACCTACTTAGGTTGGTT+TGG | + | chr7.2:24224078-24224097 | MS.gene54995:intron | 40.0% |
| ATACTGTCCCACGTATCCAA+CGG | - | chr7.2:24224760-24224779 | None:intergenic | 45.0% | |
| ATGGAGAGAAACACGTTGGA+TGG | + | chr7.2:24224778-24224797 | MS.gene54995:CDS | 45.0% | |
| CAGTATGGAGAGAAACACGT+TGG | + | chr7.2:24224774-24224793 | MS.gene54995:CDS | 45.0% | |
| CGCAGAGTTGTAACCTACTT+AGG | + | chr7.2:24224069-24224088 | MS.gene54995:intron | 45.0% | |
| CTGATGCCAAAGATTCCTTC+AGG | + | chr7.2:24223440-24223459 | MS.gene54995:CDS | 45.0% | |
| GAGTCCCAAATTGGCAAAGA+AGG | - | chr7.2:24224874-24224893 | None:intergenic | 45.0% | |
| TGCCAAAGATTCCTTCAGGA+TGG | + | chr7.2:24223444-24223463 | MS.gene54995:CDS | 45.0% | |
| TTACCTCAGCACTAGGCAAA+CGG | - | chr7.2:24224276-24224295 | None:intergenic | 45.0% | |
| ! | AGATTTGGGTTCCATCCTGA+AGG | - | chr7.2:24223458-24223477 | None:intergenic | 45.0% |
| !!! | ATATTTTTTTTTATGTAAAT+CGG | + | chr7.2:24224036-24224055 | MS.gene54995:intron | 5.0% |
| GTTGGATACGTGGGACAGTA+TGG | + | chr7.2:24224759-24224778 | MS.gene54995:CDS | 50.0% | |
| !! | ATGACGGTGTTGCTGATAGC+TGG | + | chr7.2:24224717-24224736 | MS.gene54995:CDS | 50.0% |
| TCTCCGTTTGCCTAGTGCTG+AGG | + | chr7.2:24224270-24224289 | MS.gene54995:intron | 55.0% | |
| AGCCACAGCCGTTGGATACG+TGG | + | chr7.2:24224749-24224768 | MS.gene54995:CDS | 60.0% | |
| GCCACAGCCGTTGGATACGT+GGG | + | chr7.2:24224750-24224769 | MS.gene54995:CDS | 60.0% | |
| TCCCACGTATCCAACGGCTG+TGG | - | chr7.2:24224754-24224773 | None:intergenic | 60.0% | |
| TGTTCAGCAGCCACAGCCGT+TGG | + | chr7.2:24224741-24224760 | MS.gene54995:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 24223436 | 24224926 | 24223436 | ID=MS.gene54995 |
| chr7.2 | mRNA | 24223436 | 24224926 | 24223436 | ID=MS.gene54995.t1;Parent=MS.gene54995 |
| chr7.2 | exon | 24223436 | 24223656 | 24223436 | ID=MS.gene54995.t1.exon1;Parent=MS.gene54995.t1 |
| chr7.2 | CDS | 24223436 | 24223656 | 24223436 | ID=cds.MS.gene54995.t1;Parent=MS.gene54995.t1 |
| chr7.2 | exon | 24223776 | 24223902 | 24223776 | ID=MS.gene54995.t1.exon2;Parent=MS.gene54995.t1 |
| chr7.2 | CDS | 24223776 | 24223902 | 24223776 | ID=cds.MS.gene54995.t1;Parent=MS.gene54995.t1 |
| chr7.2 | exon | 24224714 | 24224926 | 24224714 | ID=MS.gene54995.t1.exon3;Parent=MS.gene54995.t1 |
| chr7.2 | CDS | 24224714 | 24224926 | 24224714 | ID=cds.MS.gene54995.t1;Parent=MS.gene54995.t1 |
| Gene Sequence |
| Protein sequence |
