Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55409.t1 | XP_003614060.2 | 96.5 | 604 | 21 | 0 | 1 | 604 | 1 | 604 | 0.00E+00 | 1166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55409.t1 | Q9FZD1 | 62.8 | 573 | 202 | 3 | 39 | 600 | 51 | 623 | 7.0e-211 | 734.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55409.t1 | G7JWU3 | 96.5 | 604 | 21 | 0 | 1 | 604 | 1 | 604 | 0.0e+00 | 1166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene55409 | MS.gene36441 | PPI |
| MS.gene35110 | MS.gene55409 | PPI |
| MS.gene55409 | MS.gene73119 | PPI |
| MS.gene55409 | MS.gene35108 | PPI |
| MS.gene55409 | MS.gene28721 | PPI |
| MS.gene55409 | MS.gene028350 | PPI |
| MS.gene035805 | MS.gene55409 | PPI |
| MS.gene55409 | MS.gene064542 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55409.t1 | MTR_5g044270 | 96.523 | 604 | 21 | 0 | 1 | 604 | 1 | 604 | 0.0 | 1212 |
| MS.gene55409.t1 | MTR_6g024415 | 95.413 | 109 | 5 | 0 | 287 | 395 | 3 | 111 | 2.34e-65 | 211 |
| MS.gene55409.t1 | MTR_1g009040 | 60.000 | 155 | 39 | 4 | 5 | 158 | 1 | 133 | 1.54e-49 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55409.t1 | AT1G26460 | 60.835 | 623 | 221 | 7 | 1 | 600 | 1 | 623 | 0.0 | 779 |
Find 136 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCAATTGTTTCTACTTT+TGG | 0.135748 | 5.2:-34580249 | MS.gene55409:CDS |
| ATTTGTTTAATCATTATATT+AGG | 0.156430 | 5.2:-34583644 | MS.gene55409:CDS |
| CTATTATGGCCTGGAGTTTA+TGG | 0.184716 | 5.2:-34581036 | MS.gene55409:CDS |
| GATTGATTTGATTGTGAAAT+TGG | 0.186157 | 5.2:+34583992 | None:intergenic |
| TGAAGGATGGGTTAGGAGTT+TGG | 0.215682 | 5.2:-34583700 | MS.gene55409:CDS |
| AGGAGCAGCATGGGCTGTTT+TGG | 0.238246 | 5.2:-34580898 | MS.gene55409:CDS |
| AAATTAACATCAGGTTTATT+AGG | 0.243041 | 5.2:+34583663 | None:intergenic |
| AATGGATGTAAAGAAGTAAA+TGG | 0.243768 | 5.2:+34580714 | None:intergenic |
| CCCCACTCTACAACGAAAAT+TGG | 0.270895 | 5.2:-34583872 | MS.gene55409:CDS |
| TTTGAGCATGCAACAGATAA+TGG | 0.279170 | 5.2:+34580696 | None:intergenic |
| TCCTAGCAGCTCAGAATTAT+AGG | 0.283390 | 5.2:+34580917 | None:intergenic |
| GTTTGGGCACATAGTAAGTT+TGG | 0.305274 | 5.2:-34576798 | MS.gene55409:CDS |
| TCGGGTTCTTGATTGAGATA+TGG | 0.306238 | 5.2:+34583960 | None:intergenic |
| CATAAACTCCAGGCCATAAT+AGG | 0.307963 | 5.2:+34581037 | None:intergenic |
| AATAGCAAATTAGCCTATTA+TGG | 0.308171 | 5.2:-34581050 | MS.gene55409:CDS |
| CATGATTATGAGCAAGCTTA+TGG | 0.308715 | 5.2:-34580771 | MS.gene55409:CDS |
| CGTGAAGTTTCAAAGGTGTT+TGG | 0.315096 | 5.2:-34576815 | MS.gene55409:CDS |
| GCGAGATAAGATTCTGGATT+TGG | 0.315401 | 5.2:+34580849 | None:intergenic |
| AAACAATTGATTCAAAAGTT+TGG | 0.321687 | 5.2:+34580259 | None:intergenic |
| CAAAAGCGCGTTGGAATCAT+AGG | 0.323561 | 5.2:+34583779 | None:intergenic |
| ATCTTTGCGAGATAAGATTC+TGG | 0.329322 | 5.2:+34580843 | None:intergenic |
| TCTGTTGGTGGTGGTGGTGT+GGG | 0.334079 | 5.2:+34583927 | None:intergenic |
| TATGAAAATGCACAATTTCC+AGG | 0.338364 | 5.2:+34581097 | None:intergenic |
| GGGGATCCTGAAGCTGGATT+TGG | 0.341174 | 5.2:+34583891 | None:intergenic |
| GATATATGCCCTTGCATCAT+TGG | 0.349384 | 5.2:-34580823 | MS.gene55409:CDS |
| ACTTCATCTTTAACCCCATT+TGG | 0.351693 | 5.2:-34583831 | MS.gene55409:CDS |
| ATTTGACATCCCGGTTAATC+AGG | 0.351971 | 5.2:+34576729 | None:intergenic |
| TGGTGTGGGGTCTACAAGTT+CGG | 0.354920 | 5.2:+34583941 | None:intergenic |
| GCGCTAAACTGTATCATTGT+AGG | 0.358136 | 5.2:-34580315 | MS.gene55409:CDS |
| CCATTGTGCGAATGAATATT+AGG | 0.358922 | 5.2:+34580219 | None:intergenic |
| GAATCATTTGACTTGGTCAT+TGG | 0.359618 | 5.2:-34581413 | MS.gene55409:CDS |
| ATTAGGGCTAATCTTATGAT+TGG | 0.367116 | 5.2:-34583627 | MS.gene55409:CDS |
| AAATTAAAACTCGCTGTATT+CGG | 0.367896 | 5.2:+34583546 | None:intergenic |
| TCGATGCCCACCTGATTAAC+CGG | 0.368565 | 5.2:-34576739 | MS.gene55409:CDS |
| TATGGGAGTGCTTGATGATA+TGG | 0.373361 | 5.2:-34576702 | MS.gene55409:intron |
| AGGCAATACGACTTTGCATT+AGG | 0.374621 | 5.2:+34576767 | None:intergenic |
| TTTGGGCACATAGTAAGTTT+GGG | 0.376407 | 5.2:-34576797 | MS.gene55409:CDS |
| CGGGTTCTTGATTGAGATAT+GGG | 0.376922 | 5.2:+34583961 | None:intergenic |
| TGCATTTGGGAAGGCCAATA+AGG | 0.377932 | 5.2:-34580187 | MS.gene55409:intron |
| AGTAAGATCCATCACCTTAT+TGG | 0.380285 | 5.2:+34580173 | None:intergenic |
| ACAAAGCCCTTTGCCCCTCC+TGG | 0.381258 | 5.2:-34581115 | MS.gene55409:CDS |
| TTGTAGGATCTGCGAACATA+TGG | 0.381386 | 5.2:-34580299 | MS.gene55409:CDS |
| TTCTGTTGGTGGTGGTGGTG+TGG | 0.384006 | 5.2:+34583926 | None:intergenic |
| GATCCTGAAGCTGGATTTGG+TGG | 0.389737 | 5.2:+34583894 | None:intergenic |
| TTTGTTTAATCATTATATTA+GGG | 0.392061 | 5.2:-34583643 | MS.gene55409:CDS |
| GCTTGATTTGTTAGCTCAGA+TGG | 0.397920 | 5.2:-34583586 | MS.gene55409:CDS |
| CTCCCACCAAATCCAGCTTC+AGG | 0.401623 | 5.2:-34583897 | MS.gene55409:CDS |
| GTGAAGTTTCAAAGGTGTTT+GGG | 0.404120 | 5.2:-34576814 | MS.gene55409:CDS |
| GTTATCAGGATGCTGCAATC+TGG | 0.406078 | 5.2:-34581458 | MS.gene55409:intron |
| TCTTAATTACAGTACTGAAA+TGG | 0.406262 | 5.2:-34576437 | MS.gene55409:CDS |
| GGTTCTTAAGGCTATGCATC+AGG | 0.418948 | 5.2:-34583523 | MS.gene55409:CDS |
| AAGTCAAATGATTCATCATC+AGG | 0.420113 | 5.2:+34581422 | None:intergenic |
| AATGGGCTGATGTATGCATT+TGG | 0.421515 | 5.2:-34580201 | MS.gene55409:CDS |
| ATTTATTTGCAGATAGCTGC+TGG | 0.422592 | 5.2:-34576607 | MS.gene55409:intron |
| GGCCATCTACAAAAGGCATT+TGG | 0.424516 | 5.2:-34580801 | MS.gene55409:CDS |
| ATCTGTTGCATGCTCAAAGA+AGG | 0.425706 | 5.2:-34580691 | MS.gene55409:CDS |
| ATTGATTTGATTGTGAAATT+GGG | 0.427484 | 5.2:+34583993 | None:intergenic |
| GATGATTCTGTTGGTGGTGG+TGG | 0.436566 | 5.2:+34583921 | None:intergenic |
| ATGGATGTAAAGAAGTAAAT+GGG | 0.436831 | 5.2:+34580715 | None:intergenic |
| GGTGTGGGGTCTACAAGTTC+GGG | 0.441233 | 5.2:+34583942 | None:intergenic |
| CTAATATTCATTCGCACAAT+GGG | 0.441539 | 5.2:-34580218 | MS.gene55409:CDS |
| CACTGAAAGCGATGAAAGAC+TGG | 0.442968 | 5.2:-34576524 | MS.gene55409:CDS |
| TTGGGTAGCTTCACAACGTT+GGG | 0.445343 | 5.2:-34583742 | MS.gene55409:CDS |
| CGATCTTACAATTACCGACT+TGG | 0.449042 | 5.2:-34576490 | MS.gene55409:CDS |
| ATATATGCCCTTGCATCATT+GGG | 0.454900 | 5.2:-34580822 | MS.gene55409:CDS |
| AAATGTTTGAAGGATGGGTT+AGG | 0.459710 | 5.2:-34583707 | MS.gene55409:CDS |
| GTGTTGCTTTCTGTAGATGA+AGG | 0.461555 | 5.2:-34580975 | MS.gene55409:CDS |
| GATTAAACAGCATGTTTCGT+CGG | 0.463811 | 5.2:+34576458 | None:intergenic |
| ATGGCTCGATGGATTGTAAA+GGG | 0.468300 | 5.2:-34581017 | MS.gene55409:CDS |
| TGATTAAACAAATTAACATC+AGG | 0.470842 | 5.2:+34583654 | None:intergenic |
| GGGAGTGATGATTCTGTTGG+TGG | 0.473703 | 5.2:+34583915 | None:intergenic |
| CAATTTCCAGGAGGGGCAAA+GGG | 0.475476 | 5.2:+34581109 | None:intergenic |
| CCGGGATGTCAAATCTGCTA+TGG | 0.480468 | 5.2:-34576720 | MS.gene55409:CDS |
| AGCGCAGCAACAGACTTGTA+TGG | 0.481918 | 5.2:+34580333 | None:intergenic |
| TAGAGTGGGGATCCTGAAGC+TGG | 0.482043 | 5.2:+34583885 | None:intergenic |
| ATTAAACAGCATGTTTCGTC+GGG | 0.488375 | 5.2:+34576459 | None:intergenic |
| ATGGGCTGATGTATGCATTT+GGG | 0.492973 | 5.2:-34580200 | MS.gene55409:CDS |
| TCAGGTGGGCATCGATAAGC+AGG | 0.497019 | 5.2:+34576747 | None:intergenic |
| TGAAGGAAATGTTTGAAGGA+TGG | 0.497761 | 5.2:-34583713 | MS.gene55409:CDS |
| AGTGATGATTCTGTTGGTGG+TGG | 0.500044 | 5.2:+34583918 | None:intergenic |
| CTGTTGGTGGTGGTGGTGTG+GGG | 0.502808 | 5.2:+34583928 | None:intergenic |
| CCTAATATTCATTCGCACAA+TGG | 0.508619 | 5.2:-34580219 | MS.gene55409:CDS |
| ACAAGGAAGGCTTGATACAT+TGG | 0.516589 | 5.2:-34581273 | MS.gene55409:CDS |
| GGTGGGAGTGATGATTCTGT+TGG | 0.523245 | 5.2:+34583912 | None:intergenic |
| ATCATTGGGCCATCTACAAA+AGG | 0.524550 | 5.2:-34580808 | MS.gene55409:CDS |
| GCGCGTGTGAAATGACCAAA+TGG | 0.529121 | 5.2:+34583816 | None:intergenic |
| CAAATTAGCCTATTATGGCC+TGG | 0.529386 | 5.2:-34581045 | MS.gene55409:CDS |
| AAGTTTGGTAAGCTCGATCA+AGG | 0.529861 | 5.2:+34580274 | None:intergenic |
| ACAATTTCCAGGAGGGGCAA+AGG | 0.530807 | 5.2:+34581108 | None:intergenic |
| CTGGATTTGAACCGATGAAA+GGG | 0.534065 | 5.2:-34576588 | MS.gene55409:CDS |
| GTGTCAGCTCTGATGACTGC+AGG | 0.542612 | 5.2:-34580945 | MS.gene55409:CDS |
| CGGGATGTCAAATCTGCTAT+GGG | 0.544855 | 5.2:-34576719 | MS.gene55409:CDS |
| TGGCAATAATTGAAAAGTGC+AGG | 0.545019 | 5.2:-34581250 | MS.gene55409:CDS |
| GCAGACACGTGAAGTTTCAA+AGG | 0.545561 | 5.2:-34576822 | MS.gene55409:intron |
| CTGCTGAGAAACTTCTTGAA+CGG | 0.549508 | 5.2:-34583485 | MS.gene55409:intron |
| GCTGGATTTGAACCGATGAA+AGG | 0.552778 | 5.2:-34576589 | MS.gene55409:CDS |
| CGATGCCCACCTGATTAACC+GGG | 0.553923 | 5.2:-34576738 | MS.gene55409:CDS |
| GATGTGAAGGAAATGTTTGA+AGG | 0.554620 | 5.2:-34583717 | MS.gene55409:CDS |
| TCTGTTGCATGCTCAAAGAA+GGG | 0.556751 | 5.2:-34580690 | MS.gene55409:CDS |
| CTGAGCTGCTAGGAGCAGCA+TGG | 0.557087 | 5.2:-34580908 | MS.gene55409:CDS |
| TATGGCTCGATGGATTGTAA+AGG | 0.558390 | 5.2:-34581018 | MS.gene55409:CDS |
| GCCTGGAGTTTATGGCTCGA+TGG | 0.561213 | 5.2:-34581028 | MS.gene55409:CDS |
| ATCCTGAAGCTGGATTTGGT+GGG | 0.565034 | 5.2:+34583895 | None:intergenic |
| TGATGATGAATCATTTGACT+TGG | 0.565954 | 5.2:-34581420 | MS.gene55409:CDS |
| CGCGTGTGAAATGACCAAAT+GGG | 0.566353 | 5.2:+34583817 | None:intergenic |
| TGAGCTGCTAGGAGCAGCAT+GGG | 0.569572 | 5.2:-34580907 | MS.gene55409:CDS |
| TCCATCGAGCCATAAACTCC+AGG | 0.571109 | 5.2:+34581027 | None:intergenic |
| TGTAGGATCTGCGAACATAT+GGG | 0.572027 | 5.2:-34580298 | MS.gene55409:CDS |
| GAAGGAAATGTTTGAAGGAT+GGG | 0.576137 | 5.2:-34583712 | MS.gene55409:CDS |
| TGACATCCCGGTTAATCAGG+TGG | 0.581779 | 5.2:+34576732 | None:intergenic |
| GTAGATGGCCCAATGATGCA+AGG | 0.582438 | 5.2:+34580814 | None:intergenic |
| ACCTATAATTCTGAGCTGCT+AGG | 0.584992 | 5.2:-34580918 | MS.gene55409:CDS |
| ATTGGGTAGCTTCACAACGT+TGG | 0.592801 | 5.2:-34583743 | MS.gene55409:CDS |
| CTTAAGGCTATGCATCAGGC+TGG | 0.602664 | 5.2:-34583519 | MS.gene55409:CDS |
| AAAATGCACAATTTCCAGGA+GGG | 0.604881 | 5.2:+34581101 | None:intergenic |
| AGGAAGGCTTGATACATTGG+TGG | 0.606481 | 5.2:-34581270 | MS.gene55409:CDS |
| GACATCCCGGTTAATCAGGT+GGG | 0.607363 | 5.2:+34576733 | None:intergenic |
| ACAACGTTGGGATGATGTGA+AGG | 0.611093 | 5.2:-34583730 | MS.gene55409:CDS |
| AAATGCACAATTTCCAGGAG+GGG | 0.611198 | 5.2:+34581102 | None:intergenic |
| ACATGGTAACACGTTGTCAG+TGG | 0.611871 | 5.2:-34581330 | MS.gene55409:CDS |
| CCATAGCAGATTTGACATCC+CGG | 0.613360 | 5.2:+34576720 | None:intergenic |
| TGCAGTCATCAGAGCTGACA+CGG | 0.624877 | 5.2:+34580947 | None:intergenic |
| TAGATGGCCCAATGATGCAA+GGG | 0.631417 | 5.2:+34580815 | None:intergenic |
| GGAGTTGTGTCAAACAAGGA+AGG | 0.640031 | 5.2:-34581286 | MS.gene55409:CDS |
| AGCTGAAGTTGCAATTCGAG+CGG | 0.642238 | 5.2:-34581075 | MS.gene55409:CDS |
| GTCGGGCTTCAGAACCAAGT+CGG | 0.648802 | 5.2:+34576476 | None:intergenic |
| GCGTGTGAAATGACCAAATG+GGG | 0.653600 | 5.2:+34583818 | None:intergenic |
| ACTTGTATGGACGCTCAGCA+TGG | 0.661731 | 5.2:+34580346 | None:intergenic |
| TCATCTACAGAAAGCAACAC+AGG | 0.661970 | 5.2:+34580978 | None:intergenic |
| GGCAATAATTGAAAAGTGCA+GGG | 0.665279 | 5.2:-34581249 | MS.gene55409:intron |
| ATAGATTCAACTCTAAAACA+TGG | 0.670661 | 5.2:-34581347 | MS.gene55409:CDS |
| GTGAGGAGTTGTGTCAAACA+AGG | 0.685959 | 5.2:-34581290 | MS.gene55409:CDS |
| ATTTATTTATGAAATGTGTG+AGG | 0.693600 | 5.2:-34581307 | MS.gene55409:CDS |
| GCTGATGTATGCATTTGGGA+AGG | 0.709918 | 5.2:-34580196 | MS.gene55409:CDS |
| GAAAATGCACAATTTCCAGG+AGG | 0.712171 | 5.2:+34581100 | None:intergenic |
| TCTACAGAAAGCAACACAGG+AGG | 0.717369 | 5.2:+34580981 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 34576432 | 34584066 | 34576432 | ID=MS.gene55409 |
| chr5.2 | mRNA | 34576432 | 34584066 | 34576432 | ID=MS.gene55409.t1;Parent=MS.gene55409 |
| chr5.2 | exon | 34583486 | 34584066 | 34583486 | ID=MS.gene55409.t1.exon1;Parent=MS.gene55409.t1 |
| chr5.2 | CDS | 34583486 | 34584066 | 34583486 | ID=cds.MS.gene55409.t1;Parent=MS.gene55409.t1 |
| chr5.2 | exon | 34581250 | 34581472 | 34581250 | ID=MS.gene55409.t1.exon2;Parent=MS.gene55409.t1 |
| chr5.2 | CDS | 34581250 | 34581472 | 34581250 | ID=cds.MS.gene55409.t1;Parent=MS.gene55409.t1 |
| chr5.2 | exon | 34580671 | 34581150 | 34580671 | ID=MS.gene55409.t1.exon3;Parent=MS.gene55409.t1 |
| chr5.2 | CDS | 34580671 | 34581150 | 34580671 | ID=cds.MS.gene55409.t1;Parent=MS.gene55409.t1 |
| chr5.2 | exon | 34580188 | 34580394 | 34580188 | ID=MS.gene55409.t1.exon4;Parent=MS.gene55409.t1 |
| chr5.2 | CDS | 34580188 | 34580394 | 34580188 | ID=cds.MS.gene55409.t1;Parent=MS.gene55409.t1 |
| chr5.2 | exon | 34576703 | 34576840 | 34576703 | ID=MS.gene55409.t1.exon5;Parent=MS.gene55409.t1 |
| chr5.2 | CDS | 34576703 | 34576840 | 34576703 | ID=cds.MS.gene55409.t1;Parent=MS.gene55409.t1 |
| chr5.2 | exon | 34576432 | 34576617 | 34576432 | ID=MS.gene55409.t1.exon6;Parent=MS.gene55409.t1 |
| chr5.2 | CDS | 34576432 | 34576617 | 34576432 | ID=cds.MS.gene55409.t1;Parent=MS.gene55409.t1 |
| Gene Sequence |
| Protein sequence |
