Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55655.t1 | XP_004504355.1 | 95.4 | 370 | 17 | 0 | 1 | 370 | 20 | 389 | 1.60E-204 | 721.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55655.t1 | P48406 | 93.5 | 370 | 23 | 1 | 1 | 370 | 20 | 388 | 4.3e-203 | 708.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55655.t1 | A0A1S2YG89 | 95.4 | 370 | 17 | 0 | 1 | 370 | 20 | 389 | 1.1e-204 | 721.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052870 | MS.gene55655 | 0.803288 | 3.75E-49 | -1.69E-46 |
| MS.gene052872 | MS.gene55655 | 0.803974 | 2.70E-49 | -1.69E-46 |
| MS.gene056898 | MS.gene55655 | 0.804217 | 2.40E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55655.t1 | MTR_3g083910 | 92.683 | 369 | 27 | 0 | 1 | 369 | 20 | 388 | 0.0 | 719 |
| MS.gene55655.t1 | MTR_3g083920 | 90.786 | 369 | 34 | 0 | 1 | 369 | 20 | 388 | 0.0 | 708 |
| MS.gene55655.t1 | MTR_7g016700 | 88.108 | 370 | 44 | 0 | 1 | 370 | 20 | 389 | 0.0 | 698 |
| MS.gene55655.t1 | MTR_5g007730 | 87.838 | 370 | 45 | 0 | 1 | 370 | 20 | 389 | 0.0 | 698 |
| MS.gene55655.t1 | MTR_1g098140 | 87.838 | 370 | 45 | 0 | 1 | 370 | 20 | 389 | 0.0 | 697 |
| MS.gene55655.t1 | MTR_7g016800 | 87.838 | 370 | 45 | 0 | 1 | 370 | 20 | 389 | 0.0 | 696 |
| MS.gene55655.t1 | MTR_5g007713 | 87.568 | 370 | 46 | 0 | 1 | 370 | 20 | 389 | 0.0 | 696 |
| MS.gene55655.t1 | MTR_7g016720 | 87.838 | 370 | 45 | 0 | 1 | 370 | 20 | 389 | 0.0 | 695 |
| MS.gene55655.t1 | MTR_7g016820 | 87.568 | 370 | 46 | 0 | 1 | 370 | 20 | 389 | 0.0 | 694 |
| MS.gene55655.t1 | MTR_5g007760 | 87.297 | 370 | 47 | 0 | 1 | 370 | 20 | 389 | 0.0 | 694 |
| MS.gene55655.t1 | MTR_1g097935 | 87.568 | 370 | 46 | 0 | 1 | 370 | 20 | 389 | 0.0 | 694 |
| MS.gene55655.t1 | MTR_1g097910 | 87.297 | 370 | 47 | 0 | 1 | 370 | 20 | 389 | 0.0 | 693 |
| MS.gene55655.t1 | MTR_5g007717 | 86.757 | 370 | 49 | 0 | 1 | 370 | 20 | 389 | 0.0 | 692 |
| MS.gene55655.t1 | MTR_7g016780 | 87.297 | 370 | 47 | 0 | 1 | 370 | 20 | 389 | 0.0 | 692 |
| MS.gene55655.t1 | MTR_7g084300 | 85.135 | 370 | 55 | 0 | 1 | 370 | 20 | 389 | 0.0 | 667 |
| MS.gene55655.t1 | MTR_2g058470 | 82.432 | 370 | 65 | 0 | 1 | 370 | 20 | 389 | 0.0 | 658 |
| MS.gene55655.t1 | MTR_3g086260 | 82.114 | 369 | 66 | 0 | 1 | 369 | 20 | 388 | 0.0 | 640 |
| MS.gene55655.t1 | MTR_3g088675 | 72.162 | 370 | 103 | 0 | 1 | 370 | 20 | 389 | 0.0 | 579 |
| MS.gene55655.t1 | MTR_7g113405 | 88.119 | 303 | 36 | 0 | 68 | 370 | 1 | 303 | 0.0 | 560 |
| MS.gene55655.t1 | MTR_5g007740 | 78.221 | 326 | 71 | 0 | 45 | 370 | 1 | 326 | 0.0 | 535 |
| MS.gene55655.t1 | MTR_5g007723 | 65.217 | 391 | 83 | 5 | 1 | 370 | 40 | 398 | 3.29e-179 | 503 |
| MS.gene55655.t1 | MTR_5g007770 | 74.000 | 300 | 45 | 2 | 51 | 350 | 1 | 267 | 1.02e-160 | 451 |
| MS.gene55655.t1 | MTR_3g088670 | 49.737 | 380 | 177 | 7 | 1 | 369 | 12 | 388 | 6.66e-130 | 378 |
| MS.gene55655.t1 | MTR_4g075560 | 43.396 | 371 | 203 | 4 | 1 | 368 | 22 | 388 | 2.35e-102 | 307 |
| MS.gene55655.t1 | MTR_4g078730 | 39.402 | 368 | 216 | 2 | 1 | 368 | 29 | 389 | 9.90e-98 | 296 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55655.t1 | AT5G13930 | 84.824 | 369 | 55 | 1 | 1 | 368 | 25 | 393 | 0.0 | 654 |
| MS.gene55655.t1 | AT4G34850 | 41.398 | 372 | 214 | 3 | 1 | 370 | 23 | 392 | 2.36e-98 | 297 |
| MS.gene55655.t1 | AT4G00040 | 41.509 | 371 | 207 | 5 | 1 | 368 | 22 | 385 | 1.25e-96 | 293 |
| MS.gene55655.t1 | AT1G02050 | 41.081 | 370 | 210 | 4 | 1 | 368 | 29 | 392 | 1.67e-88 | 272 |
| MS.gene55655.t1 | AT4G34850 | 42.188 | 320 | 182 | 2 | 53 | 370 | 1 | 319 | 5.92e-86 | 263 |
| MS.gene55655.t1 | AT3G52160 | 29.388 | 245 | 149 | 11 | 131 | 368 | 97 | 324 | 1.04e-12 | 68.9 |
| MS.gene55655.t1 | AT3G52160 | 29.388 | 245 | 149 | 11 | 131 | 368 | 210 | 437 | 2.36e-12 | 68.6 |
Find 0 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTCATTGAAATATATTTC+AGG | + | 56445:2130-2149 | MS.gene55655:intron | 15.0% |
| ! | AAAAGTCTATGATTAAGAAG+CGG | + | 56445:2155-2174 | MS.gene55655:CDS | 25.0% |
| ! | GAAATGTGTTGAGTAATTAT+GGG | + | 56445:2956-2975 | MS.gene55655:CDS | 25.0% |
| ! | TATCTCAAAGAATATCGAAA+AGG | + | 56445:2795-2814 | MS.gene55655:CDS | 25.0% |
| ! | TCTTTAACAAACATTGTCTT+TGG | - | 56445:2083-2102 | MS.gene55655:intergenic | 25.0% |
| ! | TTATAATCAGATATACCCAA+AGG | - | 56445:2841-2860 | MS.gene55655:intergenic | 25.0% |
| !! | CTGATTATAACTCAATCTTT+TGG | + | 56445:2851-2870 | MS.gene55655:CDS | 25.0% |
| AGATATACCCAAAGGTTTAA+AGG | - | 56445:2833-2852 | MS.gene55655:intergenic | 30.0% | |
| ATCATATAACGTTTCACTGA+CGG | - | 56445:2430-2449 | MS.gene55655:intergenic | 30.0% | |
| CGAAATGTGTTGAGTAATTA+TGG | + | 56445:2955-2974 | MS.gene55655:CDS | 30.0% | |
| GTTATATGATGTACCAACAA+GGG | + | 56445:2440-2459 | MS.gene55655:CDS | 30.0% | |
| !! | ATATTCTTTGAGATAAGTCC+AGG | - | 56445:2790-2809 | MS.gene55655:intergenic | 30.0% |
| AAAGAATATCGAAAAGGCCT+TGG | + | 56445:2801-2820 | MS.gene55655:CDS | 35.0% | |
| AAAGATGAGATGAGTGATCT+TGG | - | 56445:2341-2360 | MS.gene55655:intergenic | 35.0% | |
| AACAAAAGCGATCAAGGAAT+GGG | + | 56445:2303-2322 | MS.gene55655:CDS | 35.0% | |
| ACGAAAGTAGTAATCAGGAT+AGG | - | 56445:1961-1980 | MS.gene55655:intergenic | 35.0% | |
| AGAAAGAAGTCCAAAGACAA+TGG | + | 56445:3021-3040 | MS.gene55655:CDS | 35.0% | |
| ATTACTCAACACATTTCGAG+TGG | - | 56445:2953-2972 | MS.gene55655:intergenic | 35.0% | |
| CGTTATATGATGTACCAACA+AGG | + | 56445:2439-2458 | MS.gene55655:CDS | 35.0% | |
| GTAACACGAAAGTAGTAATC+AGG | - | 56445:1966-1985 | MS.gene55655:intergenic | 35.0% | |
| GTTGAAGCCTTTAAACCTTT+GGG | + | 56445:2823-2842 | MS.gene55655:CDS | 35.0% | |
| TATCAGCTCACAAAATTGCT+AGG | + | 56445:2397-2416 | MS.gene55655:CDS | 35.0% | |
| TCTTCGTTTGGCTAAAGATT+TGG | + | 56445:2483-2502 | MS.gene55655:CDS | 35.0% | |
| ! | AGGTTTGAAGCCTGAAAAAT+TGG | + | 56445:2924-2943 | MS.gene55655:CDS | 35.0% |
| ACAAAAGCGATCAAGGAATG+GGG | + | 56445:2304-2323 | MS.gene55655:CDS | 40.0% | |
| ACGAGCACCTTTATTGTTCT+CGG | - | 56445:2509-2528 | MS.gene55655:intergenic | 40.0% | |
| ACTAACGTTCCATCTTCTCA+AGG | + | 56445:2759-2778 | MS.gene55655:CDS | 40.0% | |
| AGATGAGTGATCTTGGACTT+TGG | - | 56445:2334-2353 | MS.gene55655:intergenic | 40.0% | |
| AGGTTTAAAGGCTTCAACCA+AGG | - | 56445:2821-2840 | MS.gene55655:intergenic | 40.0% | |
| CAACAAAAGCGATCAAGGAA+TGG | + | 56445:2302-2321 | MS.gene55655:CDS | 40.0% | |
| GATAGTGAAGGAGCTATTGA+TGG | + | 56445:2718-2737 | MS.gene55655:CDS | 40.0% | |
| GATTTGGCCGAGAACAATAA+AGG | + | 56445:2499-2518 | MS.gene55655:CDS | 40.0% | |
| GCACTCTGATCAACACAATT+GGG | - | 56445:1939-1958 | MS.gene55655:intergenic | 40.0% | |
| GCCATGTAAGCACACATATT+GGG | - | 56445:2217-2236 | MS.gene55655:intergenic | 40.0% | |
| GCGGTACATGTATTTAACAG+AGG | + | 56445:2174-2193 | MS.gene55655:CDS | 40.0% | |
| GGTTGAAGCCTTTAAACCTT+TGG | + | 56445:2822-2841 | MS.gene55655:CDS | 40.0% | |
| GTCCACACTAACTCAAACAA+AGG | - | 56445:2679-2698 | MS.gene55655:intergenic | 40.0% | |
| TCAATAGCTCCTTCACTATC+AGG | - | 56445:2718-2737 | MS.gene55655:intergenic | 40.0% | |
| TCCCAATATGTGTGCTTACA+TGG | + | 56445:2213-2232 | MS.gene55655:CDS | 40.0% | |
| TCCCACAAGACTATCAAGAT+GGG | - | 56445:2587-2606 | MS.gene55655:intergenic | 40.0% | |
| TGCCATGTAAGCACACATAT+TGG | - | 56445:2218-2237 | MS.gene55655:intergenic | 40.0% | |
| ! | AACCTAACTTGTCCTCAACT+TGG | - | 56445:2909-2928 | MS.gene55655:intergenic | 40.0% |
| ! | ACAATCCTTCCTGATAGTGA+AGG | + | 56445:2706-2725 | MS.gene55655:CDS | 40.0% |
| ! | CTCATCTTTTGTACCACTAG+TGG | + | 56445:2352-2371 | MS.gene55655:CDS | 40.0% |
| ! | GACCTTTGTTTGAGTTAGTG+TGG | + | 56445:2674-2693 | MS.gene55655:CDS | 40.0% |
| !! | ACCCATCTTGATAGTCTTGT+GGG | + | 56445:2583-2602 | MS.gene55655:CDS | 40.0% |
| AACAAAGGTCTCTCAACCTC+AGG | - | 56445:2664-2683 | MS.gene55655:intergenic | 45.0% | |
| AACTGGTGAAGGGCTTGAAT+GGG | + | 56445:3053-3072 | MS.gene55655:CDS | 45.0% | |
| ACAGTCTCAACAGTAAGTCC+AGG | - | 56445:3099-3118 | MS.gene55655:intergenic | 45.0% | |
| AGCAGCAACAAAAGCGATCA+AGG | + | 56445:2297-2316 | MS.gene55655:CDS | 45.0% | |
| AGCGATTCTTGACCAAGTTG+AGG | + | 56445:2894-2913 | MS.gene55655:CDS | 45.0% | |
| ATCACTGCAGTCACATTTCG+TGG | + | 56445:2550-2569 | MS.gene55655:CDS | 45.0% | |
| ATTGGATGCAAGGCAAGACA+TGG | + | 56445:2243-2262 | MS.gene55655:CDS | 45.0% | |
| CAGTCTCAACAGTAAGTCCA+GGG | - | 56445:3098-3117 | MS.gene55655:intergenic | 45.0% | |
| CTCTGATCAACACAATTGGG+AGG | - | 56445:1936-1955 | MS.gene55655:intergenic | 45.0% | |
| GGCACTCTGATCAACACAAT+TGG | - | 56445:1940-1959 | MS.gene55655:intergenic | 45.0% | |
| GTCCCACAAGACTATCAAGA+TGG | - | 56445:2588-2607 | MS.gene55655:intergenic | 45.0% | |
| TAGCTCCTTCACTATCAGGA+AGG | - | 56445:2714-2733 | MS.gene55655:intergenic | 45.0% | |
| TCAACTTGGTCAAGAATCGC+TGG | - | 56445:2895-2914 | MS.gene55655:intergenic | 45.0% | |
| TCACTGCAGTCACATTTCGT+GGG | + | 56445:2551-2570 | MS.gene55655:CDS | 45.0% | |
| TCTTGCCTTGCATCCAATGA+TGG | - | 56445:2241-2260 | MS.gene55655:intergenic | 45.0% | |
| TGCTTACATGGCACCATCAT+TGG | + | 56445:2225-2244 | MS.gene55655:CDS | 45.0% | |
| TGGTTCAGATCCATTACCTG+AGG | + | 56445:2645-2664 | MS.gene55655:CDS | 45.0% | |
| ! | ATCTTTTGGATTGCTCACCC+CGG | + | 56445:2865-2884 | MS.gene55655:CDS | 45.0% |
| ! | CACCCATCTTGATAGTCTTG+TGG | + | 56445:2582-2601 | MS.gene55655:CDS | 45.0% |
| ! | CGAGTGGCTTCCAATTTTTC+AGG | - | 56445:2937-2956 | MS.gene55655:intergenic | 45.0% |
| ! | CTTGAATGGGGTGTGTTGTT+TGG | + | 56445:3066-3085 | MS.gene55655:CDS | 45.0% |
| ! | CTTTTGTACCACTAGTGGTG+TGG | + | 56445:2357-2376 | MS.gene55655:CDS | 45.0% |
| ! | GACCAAGTTGAGGACAAGTT+AGG | + | 56445:2904-2923 | MS.gene55655:CDS | 45.0% |
| ! | GCTGCTTCTTTTCCTAACCT+TGG | - | 56445:2283-2302 | MS.gene55655:intergenic | 45.0% |
| ! | TGTGGCTAGTCCATTGTCTT+TGG | - | 56445:3034-3053 | MS.gene55655:intergenic | 45.0% |
| !! | CTATCAAGATGGGTGTCACT+AGG | - | 56445:2577-2596 | MS.gene55655:intergenic | 45.0% |
| !! | TAAAGGTGCTCGTGTTCTTG+TGG | + | 56445:2516-2535 | MS.gene55655:CDS | 45.0% |
| !!! | TACCAACAAGGGTGTTTTGC+TGG | + | 56445:2451-2470 | MS.gene55655:CDS | 45.0% |
| !!! | TGGGGTGTGTTGTTTGGTTT+TGG | + | 56445:3072-3091 | MS.gene55655:CDS | 45.0% |
| ACTAGTGGTGTGGATATGCC+TGG | + | 56445:2367-2386 | MS.gene55655:CDS | 50.0% | |
| ACTGGTGAAGGGCTTGAATG+GGG | + | 56445:3054-3073 | MS.gene55655:CDS | 50.0% | |
| AGGCATATCCACACCACTAG+TGG | - | 56445:2368-2387 | MS.gene55655:intergenic | 50.0% | |
| CAACTGGTGAAGGGCTTGAA+TGG | + | 56445:3052-3071 | MS.gene55655:CDS | 50.0% | |
| CACCAGCAAAACACCCTTGT+TGG | - | 56445:2456-2475 | MS.gene55655:intergenic | 50.0% | |
| CAGGCACATCCTTGAGAAGA+TGG | - | 56445:2771-2790 | MS.gene55655:intergenic | 50.0% | |
| CATCTTCTCAAGGATGTGCC+TGG | + | 56445:2769-2788 | MS.gene55655:CDS | 50.0% | |
| GGTCTCTCAACCTCAGGTAA+TGG | - | 56445:2658-2677 | MS.gene55655:intergenic | 50.0% | |
| GTGAGCTGATAATCAGCACC+AGG | - | 56445:2388-2407 | MS.gene55655:intergenic | 50.0% | |
| TGGCACCATCATTGGATGCA+AGG | + | 56445:2233-2252 | MS.gene55655:CDS | 50.0% | |
| TGGTGGTACGGTTCTTCGTT+TGG | + | 56445:2471-2490 | MS.gene55655:CDS | 50.0% | |
| TTCAAGCCCTTCACCAGTTG+TGG | - | 56445:3052-3071 | MS.gene55655:intergenic | 50.0% | |
| ! | GACTAGCCACAACTGGTGAA+GGG | + | 56445:3043-3062 | MS.gene55655:CDS | 50.0% |
| ! | TATTGATGGTCACCTTCGCG+AGG | + | 56445:2732-2751 | MS.gene55655:CDS | 50.0% |
| !! | AGGGTGTTTTGCTGGTGGTA+CGG | + | 56445:2459-2478 | MS.gene55655:CDS | 50.0% |
| !! | CTTGTGGGACAAGCCTTGTT+TGG | + | 56445:2598-2617 | MS.gene55655:CDS | 50.0% |
| !! | GACAATGGACTAGCCACAAC+TGG | + | 56445:3036-3055 | MS.gene55655:CDS | 50.0% |
| !! | GGACAAGCCTTGTTTGGAGA+TGG | + | 56445:2604-2623 | MS.gene55655:CDS | 50.0% |
| !! | GTAGCAGAGGTACCAAGGTT+AGG | + | 56445:2268-2287 | MS.gene55655:CDS | 50.0% |
| !!! | CAACAAGGGTGTTTTGCTGG+TGG | + | 56445:2454-2473 | MS.gene55655:CDS | 50.0% |
| !!! | GTGTTGTTTGGTTTTGGCCC+TGG | + | 56445:3078-3097 | MS.gene55655:CDS | 50.0% |
| ACGTTAGTCCAACCTCGCGA+AGG | - | 56445:2747-2766 | MS.gene55655:intergenic | 55.0% | |
| AGCAGCGCCATCTCCAAACA+AGG | - | 56445:2614-2633 | MS.gene55655:intergenic | 55.0% | |
| GCAAGACATGGTGGTAGCAG+AGG | + | 56445:2255-2274 | MS.gene55655:CDS | 55.0% | |
| GGATGCAAGGCAAGACATGG+TGG | + | 56445:2246-2265 | MS.gene55655:CDS | 55.0% | |
| GTCAAGAATCGCTGGTCCAC+CGG | - | 56445:2887-2906 | MS.gene55655:intergenic | 55.0% | |
| TCAAGAATCGCTGGTCCACC+GGG | - | 56445:2886-2905 | MS.gene55655:intergenic | 55.0% | |
| ! | TTTTGGATTGCTCACCCCGG+TGG | + | 56445:2868-2887 | MS.gene55655:CDS | 55.0% |
| !! | GGACTAGCCACAACTGGTGA+AGG | + | 56445:3042-3061 | MS.gene55655:CDS | 55.0% |
| !! | TGGTGGTAGCAGAGGTACCA+AGG | + | 56445:2263-2282 | MS.gene55655:CDS | 55.0% |
| CAAGAATCGCTGGTCCACCG+GGG | - | 56445:2885-2904 | MS.gene55655:intergenic | 60.0% | |
| GATGGTCACCTTCGCGAGGT+TGG | + | 56445:2736-2755 | MS.gene55655:CDS | 60.0% | |
| GGCGCTGCTGCTGTGATCGT+TGG | + | 56445:2625-2644 | MS.gene55655:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 56445 | gene | 1912 | 3143 | 1912 | ID=MS.gene55655 |
| 56445 | mRNA | 1912 | 3143 | 1912 | ID=MS.gene55655.t1;Parent=MS.gene55655 |
| 56445 | exon | 1912 | 2032 | 1912 | ID=MS.gene55655.t1.exon1;Parent=MS.gene55655.t1 |
| 56445 | CDS | 1912 | 2032 | 1912 | ID=cds.MS.gene55655.t1;Parent=MS.gene55655.t1 |
| 56445 | exon | 2152 | 3143 | 2152 | ID=MS.gene55655.t1.exon2;Parent=MS.gene55655.t1 |
| 56445 | CDS | 2152 | 3143 | 2152 | ID=cds.MS.gene55655.t1;Parent=MS.gene55655.t1 |
| Gene Sequence |
| Protein sequence |
