Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56302.t1 | XP_024633784.1 | 99.2 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 6.40E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56302.t1 | Q19AV6 | 33.6 | 116 | 73 | 3 | 15 | 129 | 20 | 132 | 1.9e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56302.t1 | A0A396IIP6 | 99.2 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 4.6e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene56302 | 0.805378 | 1.37E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene56302 | 0.800311 | 1.53E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56302.t1 | MTR_0393s0040 | 93.750 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 2.57e-89 | 255 |
| MS.gene56302.t1 | MTR_3g010320 | 93.182 | 132 | 8 | 1 | 1 | 132 | 1 | 131 | 1.37e-86 | 248 |
| MS.gene56302.t1 | MTR_8g072380 | 62.319 | 69 | 10 | 1 | 64 | 132 | 4 | 56 | 7.28e-22 | 82.4 |
| MS.gene56302.t1 | MTR_2g093710 | 46.729 | 107 | 20 | 3 | 52 | 126 | 7 | 108 | 2.78e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56302.t1 | AT4G33925 | 67.424 | 132 | 43 | 0 | 1 | 132 | 1 | 132 | 2.01e-64 | 192 |
| MS.gene56302.t1 | AT4G33925 | 66.387 | 119 | 40 | 0 | 1 | 119 | 1 | 119 | 5.82e-57 | 173 |
Find 22 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTGGAAGGTTTATCTTTC+AGG | 0.114697 | 3.1:+5420133 | MS.gene56302:CDS |
| ACTAGATTACATTTCTTATT+TGG | 0.150291 | 3.1:+5420044 | MS.gene56302:intron |
| TCTCCATTTGTCTAGGTTAC+AGG | 0.214028 | 3.1:+5420822 | MS.gene56302:intron |
| GAGAATGAGTGCAAGTAATT+TGG | 0.305236 | 3.1:+5419752 | None:intergenic |
| GCAAGACTCGCTGCATCATT+AGG | 0.352521 | 3.1:+5421094 | MS.gene56302:CDS |
| ATCATAGAAGAAAGAATAAC+AGG | 0.376490 | 3.1:-5420895 | None:intergenic |
| CAAATTGAATCTACACAAAC+TGG | 0.395068 | 3.1:+5419795 | MS.gene56302:CDS |
| CTCCATTTGTCTAGGTTACA+GGG | 0.399853 | 3.1:+5420823 | MS.gene56302:intron |
| TTTCAGCAAAACACAGATAC+TGG | 0.406479 | 3.1:-5420863 | None:intergenic |
| TCTAGGTTACAGGGGAATCC+AGG | 0.471736 | 3.1:+5420832 | MS.gene56302:intron |
| TGAGGGAGCTACTAGAATTG+TGG | 0.522452 | 3.1:+5420076 | MS.gene56302:CDS |
| TCTATGATGTTGTCAACAGA+GGG | 0.540121 | 3.1:+5420910 | MS.gene56302:CDS |
| TTCTATGATGTTGTCAACAG+AGG | 0.568989 | 3.1:+5420909 | MS.gene56302:CDS |
| AAAATTTCTGCTGATCCTAG+TGG | 0.582691 | 3.1:+5420116 | MS.gene56302:CDS |
| TTTCTGCTGATCCTAGTGGA+AGG | 0.593857 | 3.1:+5420120 | MS.gene56302:CDS |
| AGGATCATACGTCGAAGTCA+AGG | 0.608072 | 3.1:+5421114 | MS.gene56302:CDS |
| TCCATTTGTCTAGGTTACAG+GGG | 0.612671 | 3.1:+5420824 | MS.gene56302:intron |
| CGAAGTCAAGGTATCTGATG+AGG | 0.612908 | 3.1:+5421126 | MS.gene56302:CDS |
| ATTTGGTAGCAGATCAAGTG+TGG | 0.614557 | 3.1:+5419769 | MS.gene56302:CDS |
| TGAAAGATAAACCTTCCACT+AGG | 0.644297 | 3.1:-5420131 | None:intergenic |
| ACTAGAATTGTGGATCAAAG+AGG | 0.644685 | 3.1:+5420086 | MS.gene56302:CDS |
| CTATGATGTTGTCAACAGAG+GGG | 0.760944 | 3.1:+5420911 | MS.gene56302:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAAAAAAGATGTAAA+AGG | - | chr3.1:5419966-5419985 | None:intergenic | 15.0% |
| !! | AAACAAAAAAAGATGTAAAA+GGG | - | chr3.1:5419965-5419984 | None:intergenic | 15.0% |
| !! | ATTGAAATTAAAGCAAAAAA+AGG | - | chr3.1:5419863-5419882 | None:intergenic | 15.0% |
| !! | TTGAAATTAAAGCAAAAAAA+GGG | - | chr3.1:5419862-5419881 | None:intergenic | 15.0% |
| !! | AAACAAACAAACAAAAACAA+AGG | - | chr3.1:5420192-5420211 | None:intergenic | 20.0% |
| !! | ACTAGATTACATTTCTTATT+TGG | + | chr3.1:5420044-5420063 | MS.gene56302:intron | 20.0% |
| !! | TTTCAACTATTCAATTGAAA+TGG | - | chr3.1:5420245-5420264 | None:intergenic | 20.0% |
| !!! | TTATTTGGTAAGAATTTTGA+GGG | + | chr3.1:5420059-5420078 | MS.gene56302:CDS | 20.0% |
| !!! | TTTTTGTTTTTTTGTAGCAT+AGG | + | chr3.1:5419976-5419995 | MS.gene56302:intron | 20.0% |
| ! | ACTGTGACATGAAAATAAAA+AGG | - | chr3.1:5419906-5419925 | None:intergenic | 25.0% |
| ! | ATCATAGAAGAAAGAATAAC+AGG | - | chr3.1:5420898-5420917 | None:intergenic | 25.0% |
| ! | CAAATCACAATTTGAATCAA+GGG | - | chr3.1:5420797-5420816 | None:intergenic | 25.0% |
| ! | GAAGAAAATAAAAGACAACA+AGG | - | chr3.1:5420966-5420985 | None:intergenic | 25.0% |
| !! | CAAATTTTCATGACAGTATA+AGG | + | chr3.1:5420760-5420779 | MS.gene56302:intron | 25.0% |
| !! | CTTATTTGGTAAGAATTTTG+AGG | + | chr3.1:5420058-5420077 | MS.gene56302:CDS | 25.0% |
| !! | GCATTTTCTCAAATTTGTAT+AGG | + | chr3.1:5420646-5420665 | MS.gene56302:intron | 25.0% |
| !! | GTTTGACATTTTATGTTTGT+TGG | + | chr3.1:5420009-5420028 | MS.gene56302:intron | 25.0% |
| !!! | AAAATTGATTTTGGCATGTT+TGG | - | chr3.1:5420566-5420585 | None:intergenic | 25.0% |
| !!! | TAAGGGTGTAAAATTGATTT+TGG | - | chr3.1:5420575-5420594 | None:intergenic | 25.0% |
| !!! | TGTATGTTTGATTTCACTTT+TGG | - | chr3.1:5420616-5420635 | None:intergenic | 25.0% |
| AAGGCAAAATCATTTCTACT+TGG | + | chr3.1:5420527-5420546 | MS.gene56302:intron | 30.0% | |
| AGGCAAAATCATTTCTACTT+GGG | + | chr3.1:5420528-5420547 | MS.gene56302:intron | 30.0% | |
| ATTGTCTTCTTCATTCAACA+GGG | + | chr3.1:5420217-5420236 | MS.gene56302:intron | 30.0% | |
| CAAATTGAATCTACACAAAC+TGG | + | chr3.1:5419795-5419814 | MS.gene56302:CDS | 30.0% | |
| CAAGTTAGAATCAATTCTGA+AGG | + | chr3.1:5420508-5420527 | MS.gene56302:intron | 30.0% | |
| GCAAATCACAATTTGAATCA+AGG | - | chr3.1:5420798-5420817 | None:intergenic | 30.0% | |
| TATTGTCTTCTTCATTCAAC+AGG | + | chr3.1:5420216-5420235 | MS.gene56302:intron | 30.0% | |
| ! | AAAAAGGTGCAACAAAAGTT+AGG | - | chr3.1:5419890-5419909 | None:intergenic | 30.0% |
| ! | AAAAGGTGCAACAAAAGTTA+GGG | - | chr3.1:5419889-5419908 | None:intergenic | 30.0% |
| !! | AGGAGTCAAAATTGTTTCTA+AGG | - | chr3.1:5420593-5420612 | None:intergenic | 30.0% |
| !! | ATGTTTGATTTCACTTTTGG+AGG | - | chr3.1:5420613-5420632 | None:intergenic | 30.0% |
| !! | GGAGTCAAAATTGTTTCTAA+GGG | - | chr3.1:5420592-5420611 | None:intergenic | 30.0% |
| AAAATTTCTGCTGATCCTAG+TGG | + | chr3.1:5420116-5420135 | MS.gene56302:CDS | 35.0% | |
| AATCATTTCTACTTGGGACT+TGG | + | chr3.1:5420534-5420553 | MS.gene56302:intron | 35.0% | |
| ACTAGAATTGTGGATCAAAG+AGG | + | chr3.1:5420086-5420105 | MS.gene56302:CDS | 35.0% | |
| ATCATTTCTACTTGGGACTT+GGG | + | chr3.1:5420535-5420554 | MS.gene56302:intron | 35.0% | |
| GTGTCAAACACATACATATG+TGG | + | chr3.1:5420376-5420395 | MS.gene56302:intron | 35.0% | |
| TAGTGGAAGGTTTATCTTTC+AGG | + | chr3.1:5420133-5420152 | MS.gene56302:CDS | 35.0% | |
| TCTATGATGTTGTCAACAGA+GGG | + | chr3.1:5420910-5420929 | MS.gene56302:CDS | 35.0% | |
| TGAAAGATAAACCTTCCACT+AGG | - | chr3.1:5420134-5420153 | None:intergenic | 35.0% | |
| TGCATATTCTCCATTTGTCT+AGG | + | chr3.1:5420815-5420834 | MS.gene56302:intron | 35.0% | |
| TTCTATGATGTTGTCAACAG+AGG | + | chr3.1:5420909-5420928 | MS.gene56302:CDS | 35.0% | |
| TTGTCTTCTTCATTCAACAG+GGG | + | chr3.1:5420218-5420237 | MS.gene56302:intron | 35.0% | |
| TTTCAGCAAAACACAGATAC+TGG | - | chr3.1:5420866-5420885 | None:intergenic | 35.0% | |
| ! | AGACAACAAGGAAGTGTATA+TGG | - | chr3.1:5420954-5420973 | None:intergenic | 35.0% |
| ! | ATGCCTTCAATCTAAAGTGT+CGG | - | chr3.1:5420339-5420358 | None:intergenic | 35.0% |
| ! | CATTGGTTGCATCTAATTGT+TGG | + | chr3.1:5420709-5420728 | MS.gene56302:intron | 35.0% |
| CATGTCTGTGTTTGCTTCAT+TGG | + | chr3.1:5420692-5420711 | MS.gene56302:intron | 40.0% | |
| CTATGATGTTGTCAACAGAG+GGG | + | chr3.1:5420911-5420930 | MS.gene56302:CDS | 40.0% | |
| CTCCATTTGTCTAGGTTACA+GGG | + | chr3.1:5420823-5420842 | MS.gene56302:intron | 40.0% | |
| GGCATGTTTGATATCCAACA+CGG | + | chr3.1:5420354-5420373 | MS.gene56302:intron | 40.0% | |
| TCCATTTGTCTAGGTTACAG+GGG | + | chr3.1:5420824-5420843 | MS.gene56302:intron | 40.0% | |
| TCTCCATTTGTCTAGGTTAC+AGG | + | chr3.1:5420822-5420841 | MS.gene56302:intron | 40.0% | |
| ! | ATTTGGTAGCAGATCAAGTG+TGG | + | chr3.1:5419769-5419788 | MS.gene56302:CDS | 40.0% |
| !!! | CAGATACTGGTCTTTTTTCC+TGG | - | chr3.1:5420853-5420872 | None:intergenic | 40.0% |
| AGGATCATACGTCGAAGTCA+AGG | + | chr3.1:5421114-5421133 | MS.gene56302:CDS | 45.0% | |
| GTATGTGTTTGACACCGTGT+TGG | - | chr3.1:5420371-5420390 | None:intergenic | 45.0% | |
| TCCCCTGTAACCTAGACAAA+TGG | - | chr3.1:5420828-5420847 | None:intergenic | 45.0% | |
| TGAGGGAGCTACTAGAATTG+TGG | + | chr3.1:5420076-5420095 | MS.gene56302:CDS | 45.0% | |
| TTTCTGCTGATCCTAGTGGA+AGG | + | chr3.1:5420120-5420139 | MS.gene56302:CDS | 45.0% | |
| ! | CGAAGTCAAGGTATCTGATG+AGG | + | chr3.1:5421126-5421145 | MS.gene56302:CDS | 45.0% |
| ! | GCACCGACACTTTAGATTGA+AGG | + | chr3.1:5420333-5420352 | MS.gene56302:intron | 45.0% |
| !! | TTAAAATATATTAATATTGT+TGG | + | chr3.1:5420732-5420751 | MS.gene56302:intron | 5.0% |
| GCAAGACTCGCTGCATCATT+AGG | + | chr3.1:5421094-5421113 | MS.gene56302:CDS | 50.0% | |
| TCTAGGTTACAGGGGAATCC+AGG | + | chr3.1:5420832-5420851 | MS.gene56302:intron | 50.0% | |
| GAGGGGAGCAACTCTGTGTA+AGG | + | chr3.1:5420928-5420947 | MS.gene56302:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 5419756 | 5421177 | 5419756 | ID=MS.gene56302 |
| chr3.1 | mRNA | 5419756 | 5421177 | 5419756 | ID=MS.gene56302.t1;Parent=MS.gene56302 |
| chr3.1 | exon | 5419756 | 5419816 | 5419756 | ID=MS.gene56302.t1.exon1;Parent=MS.gene56302.t1 |
| chr3.1 | CDS | 5419756 | 5419816 | 5419756 | ID=cds.MS.gene56302.t1;Parent=MS.gene56302.t1 |
| chr3.1 | exon | 5419925 | 5419946 | 5419925 | ID=MS.gene56302.t1.exon2;Parent=MS.gene56302.t1 |
| chr3.1 | CDS | 5419925 | 5419946 | 5419925 | ID=cds.MS.gene56302.t1;Parent=MS.gene56302.t1 |
| chr3.1 | exon | 5420049 | 5420154 | 5420049 | ID=MS.gene56302.t1.exon3;Parent=MS.gene56302.t1 |
| chr3.1 | CDS | 5420049 | 5420154 | 5420049 | ID=cds.MS.gene56302.t1;Parent=MS.gene56302.t1 |
| chr3.1 | exon | 5420837 | 5420944 | 5420837 | ID=MS.gene56302.t1.exon4;Parent=MS.gene56302.t1 |
| chr3.1 | CDS | 5420837 | 5420944 | 5420837 | ID=cds.MS.gene56302.t1;Parent=MS.gene56302.t1 |
| chr3.1 | exon | 5421076 | 5421177 | 5421076 | ID=MS.gene56302.t1.exon5;Parent=MS.gene56302.t1 |
| chr3.1 | CDS | 5421076 | 5421177 | 5421076 | ID=cds.MS.gene56302.t1;Parent=MS.gene56302.t1 |
| Gene Sequence |
| Protein sequence |
