Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56345.t1 | XP_003598365.1 | 97.5 | 325 | 6 | 1 | 1 | 323 | 1 | 325 | 4.60E-176 | 627.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56345.t1 | G7IVH8 | 97.5 | 325 | 6 | 1 | 1 | 323 | 1 | 325 | 3.3e-176 | 627.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055580 | MS.gene56345 | -0.800391 | 1.47E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56345.t1 | MTR_3g010800 | 97.538 | 325 | 6 | 1 | 1 | 323 | 1 | 325 | 0.0 | 659 |
| MS.gene56345.t1 | MTR_3g062840 | 35.494 | 324 | 176 | 14 | 7 | 318 | 8 | 310 | 8.03e-40 | 142 |
| MS.gene56345.t1 | MTR_5g075750 | 33.750 | 320 | 188 | 12 | 1 | 318 | 4 | 301 | 1.10e-38 | 139 |
| MS.gene56345.t1 | MTR_8g461170 | 29.874 | 318 | 192 | 10 | 8 | 318 | 1 | 294 | 3.31e-30 | 116 |
| MS.gene56345.t1 | MTR_7g074120 | 29.595 | 321 | 196 | 10 | 7 | 317 | 13 | 313 | 2.88e-28 | 111 |
| MS.gene56345.t1 | MTR_8g028165 | 27.817 | 284 | 176 | 10 | 37 | 314 | 34 | 294 | 3.55e-22 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56345.t1 | AT4G22900 | 46.765 | 340 | 150 | 9 | 1 | 317 | 1 | 332 | 2.81e-97 | 291 |
| MS.gene56345.t1 | AT4G11950 | 44.479 | 326 | 150 | 10 | 14 | 317 | 10 | 326 | 2.67e-83 | 255 |
| MS.gene56345.t1 | AT1G62981 | 42.395 | 309 | 152 | 12 | 28 | 318 | 41 | 341 | 2.16e-63 | 204 |
| MS.gene56345.t1 | AT1G62981 | 42.395 | 309 | 152 | 12 | 28 | 318 | 41 | 341 | 2.16e-63 | 204 |
| MS.gene56345.t1 | AT3G08600 | 32.886 | 298 | 160 | 13 | 33 | 313 | 37 | 311 | 2.82e-23 | 98.2 |
| MS.gene56345.t1 | AT4G23720 | 27.055 | 292 | 186 | 12 | 26 | 313 | 38 | 306 | 3.54e-19 | 86.7 |
| MS.gene56345.t1 | AT4G01140 | 28.328 | 293 | 184 | 13 | 27 | 316 | 28 | 297 | 2.53e-17 | 81.3 |
| MS.gene56345.t1 | AT1G62981 | 35.135 | 185 | 103 | 8 | 146 | 318 | 29 | 208 | 7.53e-16 | 75.5 |
Find 43 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATTGACTTAGTCTTAT+TGG | 0.092073 | 3.1:+6701978 | None:intergenic |
| GCTAAAACTCATGGACATTT+TGG | 0.170821 | 3.1:-6702047 | MS.gene56345:CDS |
| AGTACTATTGGTGCTGCTTT+AGG | 0.264423 | 3.1:-6701942 | MS.gene56345:CDS |
| CCTTCAAAACTTACACATAA+AGG | 0.290832 | 3.1:+6702125 | None:intergenic |
| TTATTGTCATTGAAATTTCA+TGG | 0.299770 | 3.1:-6702647 | MS.gene56345:CDS |
| AAGCTACCTTCAAATTTATC+AGG | 0.315618 | 3.1:-6702515 | MS.gene56345:CDS |
| CAACTTGTTTCTCCAATTGT+TGG | 0.336972 | 3.1:-6702296 | MS.gene56345:CDS |
| TAAGCTAAAATTCCAACAAT+TGG | 0.344113 | 3.1:+6702284 | None:intergenic |
| GTTGGTGTTGTTCTAGTTCC+AGG | 0.353242 | 3.1:+6701777 | None:intergenic |
| TTATGAAGAAGAAGCACTTC+AGG | 0.366104 | 3.1:-6701835 | MS.gene56345:CDS |
| GCATAGAGTATAATAATAAT+TGG | 0.373049 | 3.1:-6702361 | MS.gene56345:CDS |
| AAGGTATGGTGCAAAATTAA+AGG | 0.383254 | 3.1:-6702450 | MS.gene56345:CDS |
| AAGGAGTTTCATCTTGGTAT+TGG | 0.399934 | 3.1:-6702431 | MS.gene56345:CDS |
| TTTCAAGAATCAAGAAGAAA+AGG | 0.407881 | 3.1:-6702162 | MS.gene56345:CDS |
| TTGATGCCTGATAAATTTGA+AGG | 0.417038 | 3.1:+6702509 | None:intergenic |
| GCTTCAATTCCAAGTTGAAA+AGG | 0.422597 | 3.1:+6702227 | None:intergenic |
| ACACAAACATTTGTTGCTAA+AGG | 0.423104 | 3.1:+6702071 | None:intergenic |
| ATTGCTGTTGGGAGTACTAT+TGG | 0.428321 | 3.1:-6701954 | MS.gene56345:CDS |
| GCACTTCAGGTTTCAATGGT+TGG | 0.434477 | 3.1:-6701822 | MS.gene56345:CDS |
| AGAAGCACTTCAGGTTTCAA+TGG | 0.442190 | 3.1:-6701826 | MS.gene56345:CDS |
| GAAGGTAGCTTCACAGATTG+AGG | 0.451958 | 3.1:+6702527 | None:intergenic |
| CTTCAATTCCAAGTTGAAAA+GGG | 0.452206 | 3.1:+6702228 | None:intergenic |
| AAATTAAAGGAGTTTCATCT+TGG | 0.452708 | 3.1:-6702437 | MS.gene56345:CDS |
| AACATTACTCTTTCAATACA+TGG | 0.463603 | 3.1:+6702395 | None:intergenic |
| GTCAATGGAAAATTGCTGTT+GGG | 0.471587 | 3.1:-6701965 | MS.gene56345:CDS |
| TTTCAACTTGGAATTGAAGC+TGG | 0.480245 | 3.1:-6702224 | MS.gene56345:CDS |
| TTAGACAAAGATTTCAAGAC+AGG | 0.484697 | 3.1:-6702554 | MS.gene56345:CDS |
| GTTCTAGTTCCAGGTGCAGT+AGG | 0.488529 | 3.1:+6701786 | None:intergenic |
| TGAATGTGTAATCTCTTATG+AGG | 0.490113 | 3.1:+6702582 | None:intergenic |
| GGTTAAGAAAAGGTCAAGAA+TGG | 0.493692 | 3.1:-6701877 | MS.gene56345:CDS |
| AGCCAATAAGACTAAGTCAA+TGG | 0.499042 | 3.1:-6701980 | MS.gene56345:CDS |
| GTTTGTGTTGCTAAAACTCA+TGG | 0.503467 | 3.1:-6702056 | MS.gene56345:CDS |
| TGTGGAAGTTTAACAAGGTA+TGG | 0.509899 | 3.1:-6702464 | MS.gene56345:CDS |
| AGTCAATGGAAAATTGCTGT+TGG | 0.510007 | 3.1:-6701966 | MS.gene56345:CDS |
| TTCAAGAATCAAGAAGAAAA+GGG | 0.519947 | 3.1:-6702161 | MS.gene56345:CDS |
| AAGGTCAAGAATGGTTCAGA+TGG | 0.524303 | 3.1:-6701868 | MS.gene56345:CDS |
| TGGTTGTTAGGGTTAAGAAA+AGG | 0.555158 | 3.1:-6701887 | MS.gene56345:CDS |
| GTGAGAGCTCCTACTGCACC+TGG | 0.585262 | 3.1:-6701795 | MS.gene56345:CDS |
| TTAGATGTGGAAGTTTAACA+AGG | 0.623871 | 3.1:-6702469 | MS.gene56345:CDS |
| GATACTGTGAGATTTAGATG+TGG | 0.646729 | 3.1:-6702482 | MS.gene56345:CDS |
| TAGTCATGTTGCTCAAGTGT+TGG | 0.655160 | 3.1:+6701759 | None:intergenic |
| ATGTGTAATCTCTTATGAGG+AGG | 0.665772 | 3.1:+6702585 | None:intergenic |
| TGCAGTAAACCTTATCAACG+AGG | 0.756012 | 3.1:+6701723 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GCATAGAGTATAATAATAAT+TGG | - | chr3.1:6702062-6702081 | MS.gene56345:CDS | 20.0% |
| !! | TTATTGTCATTGAAATTTCA+TGG | - | chr3.1:6701776-6701795 | MS.gene56345:CDS | 20.0% |
| ! | AAATTAAAGGAGTTTCATCT+TGG | - | chr3.1:6701986-6702005 | MS.gene56345:CDS | 25.0% |
| ! | AACATTACTCTTTCAATACA+TGG | + | chr3.1:6702031-6702050 | None:intergenic | 25.0% |
| ! | TAAGCTAAAATTCCAACAAT+TGG | + | chr3.1:6702142-6702161 | None:intergenic | 25.0% |
| ! | TTCAAGAATCAAGAAGAAAA+GGG | - | chr3.1:6702262-6702281 | MS.gene56345:CDS | 25.0% |
| ! | TTTCAAGAATCAAGAAGAAA+AGG | - | chr3.1:6702261-6702280 | MS.gene56345:CDS | 25.0% |
| AAGCTACCTTCAAATTTATC+AGG | - | chr3.1:6701908-6701927 | MS.gene56345:CDS | 30.0% | |
| AAGGTATGGTGCAAAATTAA+AGG | - | chr3.1:6701973-6701992 | MS.gene56345:CDS | 30.0% | |
| CCTTCAAAACTTACACATAA+AGG | + | chr3.1:6702301-6702320 | None:intergenic | 30.0% | |
| TGAATGTGTAATCTCTTATG+AGG | + | chr3.1:6701844-6701863 | None:intergenic | 30.0% | |
| TTAGACAAAGATTTCAAGAC+AGG | - | chr3.1:6701869-6701888 | MS.gene56345:CDS | 30.0% | |
| TTAGATGTGGAAGTTTAACA+AGG | - | chr3.1:6701954-6701973 | MS.gene56345:CDS | 30.0% | |
| TTGATGCCTGATAAATTTGA+AGG | + | chr3.1:6701917-6701936 | None:intergenic | 30.0% | |
| ! | ACACAAACATTTGTTGCTAA+AGG | + | chr3.1:6702355-6702374 | None:intergenic | 30.0% |
| ! | CTTCAATTCCAAGTTGAAAA+GGG | + | chr3.1:6702198-6702217 | None:intergenic | 30.0% |
| ! | TTTTGTAGCAATGGTTGTTA+GGG | - | chr3.1:6702525-6702544 | MS.gene56345:CDS | 30.0% |
| ! | TTTTTGTAGCAATGGTTGTT+AGG | - | chr3.1:6702524-6702543 | MS.gene56345:CDS | 30.0% |
| !! | CCTTTATGTGTAAGTTTTGA+AGG | - | chr3.1:6702298-6702317 | MS.gene56345:CDS | 30.0% |
| !! | TTCCATTGACTTAGTCTTAT+TGG | + | chr3.1:6702448-6702467 | None:intergenic | 30.0% |
| !!! | AGGTTTACTTTTTGTAGCAA+TGG | - | chr3.1:6702516-6702535 | MS.gene56345:CDS | 30.0% |
| !!! | TGTGTAAGTTTTGAAGGAAA+TGG | - | chr3.1:6702304-6702323 | MS.gene56345:CDS | 30.0% |
| AAGGAGTTTCATCTTGGTAT+TGG | - | chr3.1:6701992-6702011 | MS.gene56345:CDS | 35.0% | |
| AGCCAATAAGACTAAGTCAA+TGG | - | chr3.1:6702443-6702462 | MS.gene56345:CDS | 35.0% | |
| AGTCAATGGAAAATTGCTGT+TGG | - | chr3.1:6702457-6702476 | MS.gene56345:CDS | 35.0% | |
| ATGTGTAATCTCTTATGAGG+AGG | + | chr3.1:6701841-6701860 | None:intergenic | 35.0% | |
| GATACTGTGAGATTTAGATG+TGG | - | chr3.1:6701941-6701960 | MS.gene56345:CDS | 35.0% | |
| GGTTAAGAAAAGGTCAAGAA+TGG | - | chr3.1:6702546-6702565 | MS.gene56345:CDS | 35.0% | |
| TGGTTGTTAGGGTTAAGAAA+AGG | - | chr3.1:6702536-6702555 | MS.gene56345:CDS | 35.0% | |
| TGTGGAAGTTTAACAAGGTA+TGG | - | chr3.1:6701959-6701978 | MS.gene56345:CDS | 35.0% | |
| TTTCAACTTGGAATTGAAGC+TGG | - | chr3.1:6702199-6702218 | MS.gene56345:CDS | 35.0% | |
| ! | AGTAGTAACCCTTTTCAACT+TGG | - | chr3.1:6702187-6702206 | MS.gene56345:CDS | 35.0% |
| ! | CAACTTGTTTCTCCAATTGT+TGG | - | chr3.1:6702127-6702146 | MS.gene56345:CDS | 35.0% |
| ! | GCTAAAACTCATGGACATTT+TGG | - | chr3.1:6702376-6702395 | MS.gene56345:CDS | 35.0% |
| ! | GCTTCAATTCCAAGTTGAAA+AGG | + | chr3.1:6702199-6702218 | None:intergenic | 35.0% |
| ! | GTCAATGGAAAATTGCTGTT+GGG | - | chr3.1:6702458-6702477 | MS.gene56345:CDS | 35.0% |
| ! | GTTTGTGTTGCTAAAACTCA+TGG | - | chr3.1:6702367-6702386 | MS.gene56345:CDS | 35.0% |
| !! | GCTTTAGGTGCTTTTCTTTT+AGG | - | chr3.1:6702496-6702515 | MS.gene56345:CDS | 35.0% |
| !! | TTATGAAGAAGAAGCACTTC+AGG | - | chr3.1:6702588-6702607 | MS.gene56345:CDS | 35.0% |
| !!! | AACTCATGGACATTTTGGTT+TGG | - | chr3.1:6702381-6702400 | MS.gene56345:CDS | 35.0% |
| AAGGTCAAGAATGGTTCAGA+TGG | - | chr3.1:6702555-6702574 | MS.gene56345:CDS | 40.0% | |
| TAGTCATGTTGCTCAAGTGT+TGG | + | chr3.1:6702667-6702686 | None:intergenic | 40.0% | |
| !! | AGAAGCACTTCAGGTTTCAA+TGG | - | chr3.1:6702597-6702616 | MS.gene56345:CDS | 40.0% |
| !! | AGTACTATTGGTGCTGCTTT+AGG | - | chr3.1:6702481-6702500 | MS.gene56345:CDS | 40.0% |
| !! | ATTGCTGTTGGGAGTACTAT+TGG | - | chr3.1:6702469-6702488 | MS.gene56345:CDS | 40.0% |
| GAAGGTAGCTTCACAGATTG+AGG | + | chr3.1:6701899-6701918 | None:intergenic | 45.0% | |
| ! | GCACTTCAGGTTTCAATGGT+TGG | - | chr3.1:6702601-6702620 | MS.gene56345:CDS | 45.0% |
| !! | GTTGGTGTTGTTCTAGTTCC+AGG | + | chr3.1:6702649-6702668 | None:intergenic | 45.0% |
| !! | GTTCTAGTTCCAGGTGCAGT+AGG | + | chr3.1:6702640-6702659 | None:intergenic | 50.0% |
| GTGAGAGCTCCTACTGCACC+TGG | - | chr3.1:6702628-6702647 | MS.gene56345:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 6701737 | 6702708 | 6701737 | ID=MS.gene56345 |
| chr3.1 | mRNA | 6701737 | 6702708 | 6701737 | ID=MS.gene56345.t1;Parent=MS.gene56345 |
| chr3.1 | exon | 6701737 | 6702708 | 6701737 | ID=MS.gene56345.t1.exon1;Parent=MS.gene56345.t1 |
| chr3.1 | CDS | 6701737 | 6702708 | 6701737 | ID=cds.MS.gene56345.t1;Parent=MS.gene56345.t1 |
| Gene Sequence |
| Protein sequence |
