Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56492.t1 | GAU32879.1 | 76.7 | 86 | 20 | 0 | 1 | 86 | 1 | 86 | 1.10E-25 | 125.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56492.t1 | A0A2Z6NMK5 | 76.7 | 86 | 20 | 0 | 1 | 86 | 1 | 86 | 8.3e-26 | 125.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056849 | MS.gene56492 | 0.803177 | 3.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56492.t1 | MTR_4g082883 | 50.667 | 75 | 32 | 2 | 16 | 86 | 26 | 99 | 7.41e-13 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGGTAGAAACTTATT+TGG | 0.193374 | 4.4:+30148441 | None:intergenic |
| GTTTCTACCAAGTGAGGTTT+TGG | 0.234806 | 4.4:-30148433 | MS.gene56492:CDS |
| AGCACACCACCTTCGGATTT+AGG | 0.251846 | 4.4:+30148261 | None:intergenic |
| GAGAAGGTGGCAACTGCTGC+AGG | 0.381310 | 4.4:-30148357 | MS.gene56492:CDS |
| TCCGAAGGTGGTGTGCTTGA+TGG | 0.441201 | 4.4:-30148255 | MS.gene56492:CDS |
| GAGTCAGCACAGACAACTGC+AGG | 0.472436 | 4.4:-30148390 | MS.gene56492:CDS |
| ACCATCAAGCACACCACCTT+CGG | 0.487811 | 4.4:+30148254 | None:intergenic |
| CTGTAGTTGAGCATGTTGAC+AGG | 0.490478 | 4.4:-30148320 | MS.gene56492:CDS |
| TCGGATTTAGGAGGCTCCTC+TGG | 0.493494 | 4.4:+30148273 | None:intergenic |
| GGTGGCAACTGCTGCAGGGG+AGG | 0.534933 | 4.4:-30148352 | MS.gene56492:CDS |
| GCACTCCCCAAAACCTCACT+TGG | 0.541506 | 4.4:+30148426 | None:intergenic |
| AAAGAAACAATGGCAGAAAG+TGG | 0.544848 | 4.4:-30148468 | None:intergenic |
| GAAGGTGGCAACTGCTGCAG+GGG | 0.556856 | 4.4:-30148355 | MS.gene56492:CDS |
| AGAAGGTGGCAACTGCTGCA+GGG | 0.562834 | 4.4:-30148356 | MS.gene56492:CDS |
| AAATAAGTTTCTACCAAGTG+AGG | 0.567992 | 4.4:-30148439 | MS.gene56492:CDS |
| TTTAGGAGGCTCCTCTGGTG+TGG | 0.573361 | 4.4:+30148278 | None:intergenic |
| ACACCACCTTCGGATTTAGG+AGG | 0.610944 | 4.4:+30148264 | None:intergenic |
| TGCAGGGAAACAATCTGAGA+AGG | 0.618025 | 4.4:-30148373 | MS.gene56492:CDS |
| AGGGAAACAATCTGAGAAGG+TGG | 0.629631 | 4.4:-30148370 | MS.gene56492:CDS |
| GAGGAGCCTCCTAAATCCGA+AGG | 0.645422 | 4.4:-30148270 | MS.gene56492:CDS |
| TCATGTTCATTCCACACCAG+AGG | 0.674914 | 4.4:-30148289 | MS.gene56492:CDS |
| GAGCCTCCTAAATCCGAAGG+TGG | 0.678300 | 4.4:-30148267 | MS.gene56492:CDS |
| TGTAGTTGAGCATGTTGACA+GGG | 0.688044 | 4.4:-30148319 | MS.gene56492:CDS |
| AGTCAGCACAGACAACTGCA+GGG | 0.705798 | 4.4:-30148389 | MS.gene56492:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAGTTTCTACCAAGTG+AGG | - | chr4.4:30148241-30148260 | MS.gene56492:CDS | 30.0% | |
| TCACTTGGTAGAAACTTATT+TGG | + | chr4.4:30148242-30148261 | None:intergenic | 30.0% | |
| ! | GATGGTGTTAAAAAAGCTGT+TGG | - | chr4.4:30148443-30148462 | MS.gene56492:CDS | 35.0% |
| ! | TTTCTACCAAGTGAGGTTTT+GGG | - | chr4.4:30148248-30148267 | MS.gene56492:CDS | 35.0% |
| GGTGTTAAAAAAGCTGTTGG+AGG | - | chr4.4:30148446-30148465 | MS.gene56492:CDS | 40.0% | |
| TGTAGTTGAGCATGTTGACA+GGG | - | chr4.4:30148361-30148380 | MS.gene56492:CDS | 40.0% | |
| ! | GTTTCTACCAAGTGAGGTTT+TGG | - | chr4.4:30148247-30148266 | MS.gene56492:CDS | 40.0% |
| ! | TTCTACCAAGTGAGGTTTTG+GGG | - | chr4.4:30148249-30148268 | MS.gene56492:CDS | 40.0% |
| AGGGAAACAATCTGAGAAGG+TGG | - | chr4.4:30148310-30148329 | MS.gene56492:CDS | 45.0% | |
| CTGTAGTTGAGCATGTTGAC+AGG | - | chr4.4:30148360-30148379 | MS.gene56492:CDS | 45.0% | |
| TCATGTTCATTCCACACCAG+AGG | - | chr4.4:30148391-30148410 | MS.gene56492:CDS | 45.0% | |
| TGCAGGGAAACAATCTGAGA+AGG | - | chr4.4:30148307-30148326 | MS.gene56492:CDS | 45.0% | |
| ACACCACCTTCGGATTTAGG+AGG | + | chr4.4:30148419-30148438 | None:intergenic | 50.0% | |
| ACCATCAAGCACACCACCTT+CGG | + | chr4.4:30148429-30148448 | None:intergenic | 50.0% | |
| AGCACACCACCTTCGGATTT+AGG | + | chr4.4:30148422-30148441 | None:intergenic | 50.0% | |
| AGTCAGCACAGACAACTGCA+GGG | - | chr4.4:30148291-30148310 | MS.gene56492:CDS | 50.0% | |
| !!! | GTTTTGGGGAGTGCAAAGGT+GGG | - | chr4.4:30148263-30148282 | MS.gene56492:CDS | 50.0% |
| !!! | TGAGGTTTTGGGGAGTGCAA+AGG | - | chr4.4:30148259-30148278 | MS.gene56492:CDS | 50.0% |
| AGAAGGTGGCAACTGCTGCA+GGG | - | chr4.4:30148324-30148343 | MS.gene56492:CDS | 55.0% | |
| GAGCCTCCTAAATCCGAAGG+TGG | - | chr4.4:30148413-30148432 | MS.gene56492:CDS | 55.0% | |
| GAGGAGCCTCCTAAATCCGA+AGG | - | chr4.4:30148410-30148429 | MS.gene56492:CDS | 55.0% | |
| GAGTCAGCACAGACAACTGC+AGG | - | chr4.4:30148290-30148309 | MS.gene56492:CDS | 55.0% | |
| GCACTCCCCAAAACCTCACT+TGG | + | chr4.4:30148257-30148276 | None:intergenic | 55.0% | |
| TCCGAAGGTGGTGTGCTTGA+TGG | - | chr4.4:30148425-30148444 | MS.gene56492:CDS | 55.0% | |
| TCGGATTTAGGAGGCTCCTC+TGG | + | chr4.4:30148410-30148429 | None:intergenic | 55.0% | |
| TTTAGGAGGCTCCTCTGGTG+TGG | + | chr4.4:30148405-30148424 | None:intergenic | 55.0% | |
| !!! | GGTTTTGGGGAGTGCAAAGG+TGG | - | chr4.4:30148262-30148281 | MS.gene56492:CDS | 55.0% |
| GAAGGTGGCAACTGCTGCAG+GGG | - | chr4.4:30148325-30148344 | MS.gene56492:CDS | 60.0% | |
| GAGAAGGTGGCAACTGCTGC+AGG | - | chr4.4:30148323-30148342 | MS.gene56492:CDS | 60.0% | |
| GGTGGCAACTGCTGCAGGGG+AGG | - | chr4.4:30148328-30148347 | MS.gene56492:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30148221 | 30148481 | 30148221 | ID=MS.gene56492 |
| chr4.4 | mRNA | 30148221 | 30148481 | 30148221 | ID=MS.gene56492.t1;Parent=MS.gene56492 |
| chr4.4 | exon | 30148221 | 30148481 | 30148221 | ID=MS.gene56492.t1.exon1;Parent=MS.gene56492.t1 |
| chr4.4 | CDS | 30148221 | 30148481 | 30148221 | ID=cds.MS.gene56492.t1;Parent=MS.gene56492.t1 |
| Gene Sequence |
| Protein sequence |
