Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57020.t1 | AES98664.2 | 57.6 | 66 | 28 | 0 | 13 | 78 | 943 | 1008 | 3.10E-12 | 81.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57020.t1 | G7JZJ5 | 57.6 | 66 | 28 | 0 | 13 | 78 | 943 | 1008 | 2.2e-12 | 81.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057469 | MS.gene57020 | 0.808587 | 2.88E-50 | -1.69E-46 |
| MS.gene05872 | MS.gene57020 | 0.822744 | 2.01E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57020.t1 | MTR_5g071310 | 57.576 | 66 | 28 | 0 | 13 | 78 | 943 | 1008 | 8.72e-21 | 85.9 |
| MS.gene57020.t1 | MTR_5g071310 | 82.353 | 34 | 6 | 0 | 70 | 103 | 944 | 977 | 7.24e-16 | 72.0 |
| MS.gene57020.t1 | MTR_5g070470 | 58.491 | 53 | 20 | 1 | 20 | 72 | 992 | 1042 | 7.46e-16 | 72.0 |
| MS.gene57020.t1 | MTR_1g007860 | 52.941 | 51 | 24 | 0 | 11 | 61 | 51 | 101 | 4.40e-13 | 63.9 |
| MS.gene57020.t1 | MTR_1g007860 | 67.647 | 34 | 11 | 0 | 73 | 106 | 57 | 90 | 2.97e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGACATTTGTTCTAATTC+TGG | 0.230046 | 5.1:+55016818 | MS.gene57020:CDS |
| GGGTGGGGTCGGCAGATAAA+TGG | 0.301324 | 5.1:+55017981 | MS.gene57020:CDS |
| ATGATGAAGACGGCTTCTTT+TGG | 0.314207 | 5.1:+55017016 | MS.gene57020:CDS |
| ATGATGAAGAGAACGTCTTT+TGG | 0.324794 | 5.1:+55018027 | MS.gene57020:CDS |
| AAGACGTTCTCTTCATCATT+AGG | 0.354386 | 5.1:-55018023 | None:intergenic |
| AAGAAGCCGTCTTCATCATT+AGG | 0.383282 | 5.1:-55017012 | None:intergenic |
| CAACAAACAACGGGATTAAC+CGG | 0.388636 | 5.1:+55016979 | MS.gene57020:CDS |
| CGGCAGATAAATGGATTAAC+TGG | 0.389659 | 5.1:+55017990 | MS.gene57020:CDS |
| ATCATTAGGTATGTCTGTTC+CGG | 0.401012 | 5.1:-55016998 | None:intergenic |
| GAATTGAATGGTTAGGACTT+AGG | 0.405260 | 5.1:+55016929 | MS.gene57020:intron |
| TCTATGCACAATAGAAAGTC+TGG | 0.445128 | 5.1:+55017116 | MS.gene57020:CDS |
| GATCATTGGGGCGTGTGGGT+CGG | 0.485426 | 5.1:+55017090 | MS.gene57020:CDS |
| GGAGAAGGTGACAATGAGTC+TGG | 0.493657 | 5.1:+55018053 | MS.gene57020:CDS |
| TGCCTTTGACACACAAGGGT+GGG | 0.502809 | 5.1:+55016954 | MS.gene57020:CDS |
| GGGTGGGGTCAACAAACAAC+GGG | 0.509248 | 5.1:+55016970 | MS.gene57020:CDS |
| GCCTTTGACACACAAGGGTG+GGG | 0.509819 | 5.1:+55016955 | MS.gene57020:CDS |
| TCGACACACAGGGGTGGGGT+CGG | 0.524559 | 5.1:+55017970 | MS.gene57020:intron |
| ACCCCACCCTTGTGTGTCAA+AGG | 0.536213 | 5.1:-55016956 | None:intergenic |
| GCTTTCGACACACAGGGGTG+GGG | 0.547007 | 5.1:+55017966 | MS.gene57020:intron |
| GCAATCCTCGATACCACCAC+TGG | 0.574989 | 5.1:-55017061 | None:intergenic |
| AGGATTGCCTTTGACACACA+AGG | 0.578167 | 5.1:+55016949 | MS.gene57020:CDS |
| AGGGTGGGGTCAACAAACAA+CGG | 0.598274 | 5.1:+55016969 | MS.gene57020:CDS |
| TTGCCTTTGACACACAAGGG+TGG | 0.618428 | 5.1:+55016953 | MS.gene57020:CDS |
| GAATTAGAACAAATGTCCAT+AGG | 0.622691 | 5.1:-55016815 | None:intergenic |
| TGAGTCCAGTGGTGGTATCG+AGG | 0.631616 | 5.1:+55017056 | MS.gene57020:CDS |
| GACATACCTAATGATGAAGA+CGG | 0.636658 | 5.1:+55017006 | MS.gene57020:CDS |
| GGTGACAATGAGTCCAGTGG+TGG | 0.656389 | 5.1:+55017048 | MS.gene57020:CDS |
| GGATTGCCTTTGACACACAA+GGG | 0.671237 | 5.1:+55016950 | MS.gene57020:CDS |
| GGAGGTGACAATGAGTCCAG+TGG | 0.677714 | 5.1:+55017045 | MS.gene57020:CDS |
| GAAGGTGACAATGAGTCTGG+TGG | 0.735906 | 5.1:+55018056 | MS.gene57020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTAAAAAAATTAAACT+AGG | - | chr5.1:55017878-55017897 | None:intergenic | 10.0% |
| !!! | AATATTATAAAACATCTTTT+GGG | - | chr5.1:55017832-55017851 | None:intergenic | 10.0% |
| !! | AATGAAATCAAAAATCAAAA+AGG | - | chr5.1:55017900-55017919 | None:intergenic | 15.0% |
| !! | AATTAATTAATCACATAAGA+AGG | - | chr5.1:55017723-55017742 | None:intergenic | 15.0% |
| !! | ATTAATTAATCACATAAGAA+GGG | - | chr5.1:55017722-55017741 | None:intergenic | 15.0% |
| !! | TCATTATATATTCTCATTAT+AGG | + | chr5.1:55017764-55017783 | MS.gene57020:intron | 15.0% |
| !! | TTCTAATAAATACGTATATT+TGG | - | chr5.1:55017614-55017633 | None:intergenic | 15.0% |
| !!! | AGTTTTTGAATGTAATTATA+TGG | + | chr5.1:55017230-55017249 | MS.gene57020:intron | 15.0% |
| !!! | ATATTATAAAACATCTTTTG+GGG | - | chr5.1:55017831-55017850 | None:intergenic | 15.0% |
| !!! | GAATATTATAAAACATCTTT+TGG | - | chr5.1:55017833-55017852 | None:intergenic | 15.0% |
| !!! | GTTTTATAATATTCTAATTC+AGG | + | chr5.1:55017839-55017858 | MS.gene57020:intron | 15.0% |
| !!! | GTTTTTGAATGTAATTATAT+GGG | + | chr5.1:55017231-55017250 | MS.gene57020:intron | 15.0% |
| !! | ATAATTTACAAAGAGTGAAA+TGG | + | chr5.1:55017274-55017293 | MS.gene57020:intron | 20.0% |
| !! | TGAAATGGAAATTTGAATAT+TGG | + | chr5.1:55017289-55017308 | MS.gene57020:intron | 20.0% |
| !!! | ATGCAATTACTTTATGTTTT+TGG | + | chr5.1:55017352-55017371 | MS.gene57020:intron | 20.0% |
| !!! | TATCTTTTTATGTAATGAGT+TGG | + | chr5.1:55017388-55017407 | MS.gene57020:intron | 20.0% |
| !!! | TATTATAAAACATCTTTTGG+GGG | - | chr5.1:55017830-55017849 | None:intergenic | 20.0% |
| !!! | TGCAATTACTTTATGTTTTT+GGG | + | chr5.1:55017353-55017372 | MS.gene57020:intron | 20.0% |
| !!! | TTTTAATTTTGCTGATTTTG+TGG | + | chr5.1:55016901-55016920 | MS.gene57020:intron | 20.0% |
| ! | AATATTGGTGAATGATTGTT+GGG | + | chr5.1:55017304-55017323 | MS.gene57020:intron | 25.0% |
| ! | CTACATATTAGATATTGTGA+AGG | + | chr5.1:55017458-55017477 | MS.gene57020:intron | 25.0% |
| ! | TTGAATGTAATTATATGGGT+TGG | + | chr5.1:55017235-55017254 | MS.gene57020:intron | 25.0% |
| !! | ATCGCAAAAATCAATTTTAC+TGG | + | chr5.1:55017678-55017697 | MS.gene57020:intron | 25.0% |
| !! | CAAAAATCAATTTTACTGGT+AGG | + | chr5.1:55017682-55017701 | MS.gene57020:intron | 25.0% |
| !!! | AGTAAAATTGATTTTTGCGA+TGG | - | chr5.1:55017679-55017698 | None:intergenic | 25.0% |
| !!! | TTTTAATTTTGCTGATTTCG+TGG | + | chr5.1:55017919-55017938 | MS.gene57020:intron | 25.0% |
| ATCTAATATGTAGAGTTCGA+AGG | - | chr5.1:55017451-55017470 | None:intergenic | 30.0% | |
| ATGGACATTTGTTCTAATTC+TGG | + | chr5.1:55016818-55016837 | MS.gene57020:CDS | 30.0% | |
| GAATATTGGTGAATGATTGT+TGG | + | chr5.1:55017303-55017322 | MS.gene57020:intron | 30.0% | |
| TCTAATATGTAGAGTTCGAA+GGG | - | chr5.1:55017450-55017469 | None:intergenic | 30.0% | |
| TTTGTGGAGATTGAATTGAA+TGG | + | chr5.1:55016917-55016936 | MS.gene57020:intron | 30.0% | |
| !! | ACATAAGAAGGGTGTATTTT+AGG | - | chr5.1:55017711-55017730 | None:intergenic | 30.0% |
| !! | ATAAGAAGGGTGTATTTTAG+GGG | - | chr5.1:55017709-55017728 | None:intergenic | 30.0% |
| !! | CATAAGAAGGGTGTATTTTA+GGG | - | chr5.1:55017710-55017729 | None:intergenic | 30.0% |
| AAGACGTTCTCTTCATCATT+AGG | - | chr5.1:55018026-55018045 | None:intergenic | 35.0% | |
| AATAGAAAGTCTGGTCATCA+AGG | + | chr5.1:55017125-55017144 | MS.gene57020:intron | 35.0% | |
| ATCATTAGGTATGTCTGTTC+CGG | - | chr5.1:55017001-55017020 | None:intergenic | 35.0% | |
| CTTGAGTTTCTTACTATCGT+TGG | + | chr5.1:55017521-55017540 | MS.gene57020:intron | 35.0% | |
| GAATTGAATGGTTAGGACTT+AGG | + | chr5.1:55016929-55016948 | MS.gene57020:intron | 35.0% | |
| GACATACCTAATGATGAAGA+CGG | + | chr5.1:55017006-55017025 | MS.gene57020:CDS | 35.0% | |
| GGAGATTGAATTGAATGGTT+AGG | + | chr5.1:55016922-55016941 | MS.gene57020:intron | 35.0% | |
| TAAGAAACTCAAGAGAAGTG+AGG | - | chr5.1:55017514-55017533 | None:intergenic | 35.0% | |
| TCTATGCACAATAGAAAGTC+TGG | + | chr5.1:55017116-55017135 | MS.gene57020:CDS | 35.0% | |
| TTCGTGGAGATTGAATTGAA+TGG | + | chr5.1:55017935-55017954 | MS.gene57020:intron | 35.0% | |
| ! | ATGATGAAGAGAACGTCTTT+TGG | + | chr5.1:55018027-55018046 | MS.gene57020:CDS | 35.0% |
| ! | CTCATTATAGGATGATTTGC+AGG | + | chr5.1:55017776-55017795 | MS.gene57020:intron | 35.0% |
| ! | TGATGAAGAGAACGTCTTTT+GGG | + | chr5.1:55018028-55018047 | MS.gene57020:CDS | 35.0% |
| !! | AGGATTGCGATTTTGATCAT+TGG | + | chr5.1:55017076-55017095 | MS.gene57020:CDS | 35.0% |
| !! | GATTGCGATTTTGATCATTG+GGG | + | chr5.1:55017078-55017097 | MS.gene57020:CDS | 35.0% |
| !! | GGATTGCGATTTTGATCATT+GGG | + | chr5.1:55017077-55017096 | MS.gene57020:CDS | 35.0% |
| !! | TAAGAAGGGTGTATTTTAGG+GGG | - | chr5.1:55017708-55017727 | None:intergenic | 35.0% |
| AAGAAGCCGTCTTCATCATT+AGG | - | chr5.1:55017015-55017034 | None:intergenic | 40.0% | |
| AGTCCTTACCAGAGCAAATT+TGG | + | chr5.1:55017411-55017430 | MS.gene57020:intron | 40.0% | |
| ATGGTGATTGAGGCAGATTA+AGG | - | chr5.1:55017660-55017679 | None:intergenic | 40.0% | |
| ATTAGAGGAACGATGCAAAG+AGG | + | chr5.1:55017553-55017572 | MS.gene57020:intron | 40.0% | |
| CAACAAACAACGGGATTAAC+CGG | + | chr5.1:55016979-55016998 | MS.gene57020:CDS | 40.0% | |
| CAGAACAATGATCATGGCAA+AGG | + | chr5.1:55017329-55017348 | MS.gene57020:intron | 40.0% | |
| GTCCTTACCAGAGCAAATTT+GGG | + | chr5.1:55017412-55017431 | MS.gene57020:intron | 40.0% | |
| TGCATCGTTCCTCTAATCTT+GGG | - | chr5.1:55017550-55017569 | None:intergenic | 40.0% | |
| TGGTGATTGAGGCAGATTAA+GGG | - | chr5.1:55017659-55017678 | None:intergenic | 40.0% | |
| TTACCAGAGCAAATTTGGGA+AGG | + | chr5.1:55017416-55017435 | MS.gene57020:intron | 40.0% | |
| TTAGAGGAACGATGCAAAGA+GGG | + | chr5.1:55017554-55017573 | MS.gene57020:intron | 40.0% | |
| TTGCATCGTTCCTCTAATCT+TGG | - | chr5.1:55017551-55017570 | None:intergenic | 40.0% | |
| ! | ATGATGAAGACGGCTTCTTT+TGG | + | chr5.1:55017016-55017035 | MS.gene57020:CDS | 40.0% |
| ! | GATGAAGAGAACGTCTTTTG+GGG | + | chr5.1:55018029-55018048 | MS.gene57020:CDS | 40.0% |
| ! | GATTTTTGCGATGGTGATTG+AGG | - | chr5.1:55017670-55017689 | None:intergenic | 40.0% |
| ! | TTCCCAAATTTGCTCTGGTA+AGG | - | chr5.1:55017417-55017436 | None:intergenic | 40.0% |
| !! | CGGCAGATAAATGGATTAAC+TGG | + | chr5.1:55017990-55018009 | MS.gene57020:CDS | 40.0% |
| AACGATGCAAAGAGGGAACA+GGG | + | chr5.1:55017561-55017580 | MS.gene57020:intron | 45.0% | |
| AGGATTGCCTTTGACACACA+AGG | + | chr5.1:55016949-55016968 | MS.gene57020:CDS | 45.0% | |
| AGGATTGCTTTCGACACACA+GGG | + | chr5.1:55017960-55017979 | MS.gene57020:intron | 45.0% | |
| GGATTGCCTTTGACACACAA+GGG | + | chr5.1:55016950-55016969 | MS.gene57020:CDS | 45.0% | |
| TAGGATTGCTTTCGACACAC+AGG | + | chr5.1:55017959-55017978 | MS.gene57020:intron | 45.0% | |
| TGGGTGCAGAACAATGATCA+TGG | + | chr5.1:55017323-55017342 | MS.gene57020:intron | 45.0% | |
| ! | AGACCTTCCCAAATTTGCTC+TGG | - | chr5.1:55017422-55017441 | None:intergenic | 45.0% |
| ! | TTTTGATCATTGGGGCGTGT+GGG | + | chr5.1:55017086-55017105 | MS.gene57020:CDS | 45.0% |
| !! | ATTTTGATCATTGGGGCGTG+TGG | + | chr5.1:55017085-55017104 | MS.gene57020:CDS | 45.0% |
| ACGATGCAAAGAGGGAACAG+GGG | + | chr5.1:55017562-55017581 | MS.gene57020:intron | 50.0% | |
| AGGGTGGGGTCAACAAACAA+CGG | + | chr5.1:55016969-55016988 | MS.gene57020:CDS | 50.0% | |
| GAACGATGCAAAGAGGGAAC+AGG | + | chr5.1:55017560-55017579 | MS.gene57020:intron | 50.0% | |
| GAAGGTGACAATGAGTCTGG+TGG | + | chr5.1:55018056-55018075 | MS.gene57020:CDS | 50.0% | |
| GGAGAAGGTGACAATGAGTC+TGG | + | chr5.1:55018053-55018072 | MS.gene57020:CDS | 50.0% | |
| GGATTGCTTTCGACACACAG+GGG | + | chr5.1:55017961-55017980 | MS.gene57020:intron | 50.0% | |
| TGAAGGATTGCGATGCTTGC+CGG | + | chr5.1:55017475-55017494 | MS.gene57020:intron | 50.0% | |
| TGCCTTTGACACACAAGGGT+GGG | + | chr5.1:55016954-55016973 | MS.gene57020:CDS | 50.0% | |
| TTGCCTTTGACACACAAGGG+TGG | + | chr5.1:55016953-55016972 | MS.gene57020:CDS | 50.0% | |
| ! | CGTTGGTTGCCCAAGATTAG+AGG | + | chr5.1:55017538-55017557 | MS.gene57020:intron | 50.0% |
| !! | GAAGACGGCTTCTTTTGGAG+AGG | + | chr5.1:55017021-55017040 | MS.gene57020:CDS | 50.0% |
| !! | GAAGAGAACGTCTTTTGGGG+TGG | + | chr5.1:55018032-55018051 | MS.gene57020:CDS | 50.0% |
| !!! | AACGTCTTTTGGGGTGGAGA+AGG | + | chr5.1:55018038-55018057 | MS.gene57020:CDS | 50.0% |
| AAGAGGGAACAGGGGAGGAT+TGG | + | chr5.1:55017570-55017589 | MS.gene57020:intron | 55.0% | |
| ACCCCACCCTTGTGTGTCAA+AGG | - | chr5.1:55016959-55016978 | None:intergenic | 55.0% | |
| AGAGGGAACAGGGGAGGATT+GGG | + | chr5.1:55017571-55017590 | MS.gene57020:intron | 55.0% | |
| ATGCAAAGAGGGAACAGGGG+AGG | + | chr5.1:55017565-55017584 | MS.gene57020:intron | 55.0% | |
| GCAATCCTCGATACCACCAC+TGG | - | chr5.1:55017064-55017083 | None:intergenic | 55.0% | |
| GCCTTTGACACACAAGGGTG+GGG | + | chr5.1:55016955-55016974 | MS.gene57020:CDS | 55.0% | |
| GGAGGTGACAATGAGTCCAG+TGG | + | chr5.1:55017045-55017064 | MS.gene57020:CDS | 55.0% | |
| GGGTGGGGTCAACAAACAAC+GGG | + | chr5.1:55016970-55016989 | MS.gene57020:CDS | 55.0% | |
| GGTGACAATGAGTCCAGTGG+TGG | + | chr5.1:55017048-55017067 | MS.gene57020:CDS | 55.0% | |
| TGAGTCCAGTGGTGGTATCG+AGG | + | chr5.1:55017056-55017075 | MS.gene57020:CDS | 55.0% | |
| TGCTTTCGACACACAGGGGT+GGG | + | chr5.1:55017965-55017984 | MS.gene57020:intron | 55.0% | |
| TTGCTTTCGACACACAGGGG+TGG | + | chr5.1:55017964-55017983 | MS.gene57020:intron | 55.0% | |
| !! | GACGGCTTCTTTTGGAGAGG+AGG | + | chr5.1:55017024-55017043 | MS.gene57020:CDS | 55.0% |
| !! | GGCTTCTTTTGGAGAGGAGG+AGG | + | chr5.1:55017027-55017046 | MS.gene57020:CDS | 55.0% |
| !! | GTGAGGTGTCGAATACCCTC+CGG | - | chr5.1:55017497-55017516 | None:intergenic | 55.0% |
| AGGATTGCGATGCTTGCCGG+AGG | + | chr5.1:55017478-55017497 | MS.gene57020:intron | 60.0% | |
| GATCATTGGGGCGTGTGGGT+CGG | + | chr5.1:55017090-55017109 | MS.gene57020:CDS | 60.0% | |
| GCTTTCGACACACAGGGGTG+GGG | + | chr5.1:55017966-55017985 | MS.gene57020:intron | 60.0% | |
| GGATTGCGATGCTTGCCGGA+GGG | + | chr5.1:55017479-55017498 | MS.gene57020:intron | 60.0% | |
| GGGTGGGGTCGGCAGATAAA+TGG | + | chr5.1:55017981-55018000 | MS.gene57020:CDS | 60.0% | |
| TCGACACACAGGGGTGGGGT+CGG | + | chr5.1:55017970-55017989 | MS.gene57020:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 55016818 | 55018085 | 55016818 | ID=MS.gene57020 |
| chr5.1 | mRNA | 55016818 | 55018085 | 55016818 | ID=MS.gene57020.t1;Parent=MS.gene57020 |
| chr5.1 | exon | 55016818 | 55016839 | 55016818 | ID=MS.gene57020.t1.exon1;Parent=MS.gene57020.t1 |
| chr5.1 | CDS | 55016818 | 55016839 | 55016818 | ID=cds.MS.gene57020.t1;Parent=MS.gene57020.t1 |
| chr5.1 | exon | 55016944 | 55017137 | 55016944 | ID=MS.gene57020.t1.exon2;Parent=MS.gene57020.t1 |
| chr5.1 | CDS | 55016944 | 55017137 | 55016944 | ID=cds.MS.gene57020.t1;Parent=MS.gene57020.t1 |
| chr5.1 | exon | 55017981 | 55018085 | 55017981 | ID=MS.gene57020.t1.exon3;Parent=MS.gene57020.t1 |
| chr5.1 | CDS | 55017981 | 55018085 | 55017981 | ID=cds.MS.gene57020.t1;Parent=MS.gene57020.t1 |
| Gene Sequence |
| Protein sequence |
