Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57208.t1 | XP_028762943.1 | 61.7 | 350 | 80 | 3 | 1 | 298 | 1 | 348 | 1.10E-112 | 416.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57208.t1 | A0A1U8KII2 | 56.8 | 336 | 93 | 3 | 1 | 284 | 1 | 336 | 2.1e-100 | 375.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059272 | MS.gene57208 | 0.826633 | 2.44E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57208.t1 | MTR_7g062720 | 65.909 | 132 | 45 | 0 | 1 | 132 | 1 | 132 | 2.76e-60 | 189 |
| MS.gene57208.t1 | MTR_5g061150 | 62.205 | 127 | 48 | 0 | 20 | 146 | 10 | 136 | 1.73e-48 | 158 |
| MS.gene57208.t1 | MTR_4g037355 | 50.000 | 116 | 17 | 2 | 151 | 225 | 1 | 116 | 1.96e-26 | 101 |
| MS.gene57208.t1 | MTR_4g112730 | 75.410 | 61 | 15 | 0 | 138 | 198 | 1 | 61 | 7.51e-25 | 95.9 |
| MS.gene57208.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 218 | 298 | 5 | 80 | 2.64e-23 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 5.3:-8759754 | MS.gene57208:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 5.3:+8759702 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 5.3:-8758853 | MS.gene57208:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 5.3:-8758861 | MS.gene57208:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.269782 | 5.3:-8759564 | MS.gene57208:CDS |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 5.3:-8759740 | MS.gene57208:CDS |
| ATGGTTGTATTTGCGTTTAC+AGG | 0.299146 | 5.3:-8759479 | MS.gene57208:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 5.3:+8758699 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 5.3:+8758884 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 5.3:-8758801 | MS.gene57208:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 5.3:-8759735 | MS.gene57208:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.353242 | 5.3:-8759734 | MS.gene57208:CDS |
| TTTGGGAAAATTTAAAAGAA+AGG | 0.385472 | 5.3:-8759546 | MS.gene57208:CDS |
| AACCATTCGTTACGAGCATT+AGG | 0.390171 | 5.3:+8759496 | None:intergenic |
| AGAAGATCCAAAAGATCTTT+GGG | 0.393236 | 5.3:-8759563 | MS.gene57208:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.394855 | 5.3:-8758809 | MS.gene57208:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 5.3:-8758932 | MS.gene57208:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 5.3:-8759611 | MS.gene57208:CDS |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 5.3:-8759650 | MS.gene57208:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 5.3:+8758771 | None:intergenic |
| AATGGTATCTCCATGCCCTT+CGG | 0.470113 | 5.3:+8759684 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 5.3:-8758983 | MS.gene57208:CDS |
| ATTGACACTGTGTGGAGAAA+AGG | 0.482879 | 5.3:-8759404 | MS.gene57208:CDS |
| GCTACATTCACTTCTGGGAA+TGG | 0.483138 | 5.3:+8759196 | None:intergenic |
| GCAGCAACAATACCGCGAAA+AGG | 0.486558 | 5.3:-8759320 | MS.gene57208:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 5.3:-8758852 | MS.gene57208:CDS |
| TACATTAGATGCATGAAAAG+TGG | 0.490606 | 5.3:+8759348 | None:intergenic |
| CAGCAACAATACCGCGAAAA+GGG | 0.491478 | 5.3:-8759319 | MS.gene57208:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 5.3:-8759694 | MS.gene57208:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 5.3:-8758925 | MS.gene57208:CDS |
| CATGGAGATACCATTAAAGA+AGG | 0.521442 | 5.3:-8759676 | MS.gene57208:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 5.3:-8758717 | MS.gene57208:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 5.3:-8759700 | MS.gene57208:CDS |
| AACCACACGACTCGTCCCAC+TGG | 0.556010 | 5.3:-8759226 | MS.gene57208:CDS |
| TTCCTAATGCTCGTAACGAA+TGG | 0.575736 | 5.3:-8759498 | MS.gene57208:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 5.3:-8759644 | MS.gene57208:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 5.3:-8758941 | MS.gene57208:CDS |
| ATTCGTTACGAGCATTAGGA+AGG | 0.589689 | 5.3:+8759500 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 5.3:+8759611 | None:intergenic |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 5.3:-8758938 | MS.gene57208:CDS |
| TGGGAATGGAGCACTGCCAG+TGG | 0.601752 | 5.3:+8759210 | None:intergenic |
| TGCCAGTGGGACGAGTCGTG+TGG | 0.614060 | 5.3:+8759224 | None:intergenic |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 5.3:+8758772 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 5.3:-8759699 | MS.gene57208:CDS |
| GGGAATGGAGCACTGCCAGT+GGG | 0.646793 | 5.3:+8759211 | None:intergenic |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 5.3:+8759621 | None:intergenic |
| ACTTCTCTATTGACACTGTG+TGG | 0.668278 | 5.3:-8759412 | MS.gene57208:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr5.3:8759507-8759526 | MS.gene57208:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr5.3:8759638-8759657 | MS.gene57208:CDS | 20.0% |
| !! | TTTGGGAAAATTTAAAAGAA+AGG | - | chr5.3:8758944-8758963 | MS.gene57208:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | - | chr5.3:8758750-8758769 | MS.gene57208:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | - | chr5.3:8758790-8758809 | MS.gene57208:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | - | chr5.3:8758791-8758810 | MS.gene57208:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr5.3:8759637-8759656 | MS.gene57208:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr5.3:8759681-8759700 | MS.gene57208:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr5.3:8759689-8759708 | MS.gene57208:CDS | 25.0% |
| !! | AAATTTTCCCAAAGATCTTT+TGG | + | chr5.3:8758937-8758956 | None:intergenic | 25.0% |
| !! | ATCATTTTCACATGCAAAAT+GGG | + | chr5.3:8759670-8759689 | None:intergenic | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr5.3:8759609-8759628 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | + | chr5.3:8759497-8759516 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | + | chr5.3:8758791-8758810 | None:intergenic | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | + | chr5.3:8758894-8758913 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | + | chr5.3:8759473-8759492 | None:intergenic | 25.0% |
| !!! | TGTTTTGTTTCCTTCTTTAA+TGG | + | chr5.3:8758827-8758846 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | - | chr5.3:8758927-8758946 | MS.gene57208:CDS | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | - | chr5.3:8758840-8758859 | MS.gene57208:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | - | chr5.3:8759773-8759792 | MS.gene57208:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr5.3:8759629-8759648 | MS.gene57208:CDS | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | + | chr5.3:8759145-8759164 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | - | chr5.3:8758926-8758945 | MS.gene57208:CDS | 30.0% | |
| ! | AAGATCTTTTGGATCTTCTA+TGG | + | chr5.3:8758926-8758945 | None:intergenic | 30.0% |
| ! | AGATGATCAATCTTTTCATC+AGG | + | chr5.3:8759753-8759772 | None:intergenic | 30.0% |
| ! | CATCATTTTCACATGCAAAA+TGG | + | chr5.3:8759671-8759690 | None:intergenic | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | - | chr5.3:8758756-8758775 | MS.gene57208:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | - | chr5.3:8758736-8758755 | MS.gene57208:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | + | chr5.3:8758891-8758910 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | - | chr5.3:8759423-8759442 | MS.gene57208:CDS | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr5.3:8759632-8759651 | None:intergenic | 30.0% |
| AACTGAACAACAAAAGGCTA+AGG | - | chr5.3:8758846-8758865 | MS.gene57208:CDS | 35.0% | |
| ATGGTTGTATTTGCGTTTAC+AGG | - | chr5.3:8759011-8759030 | MS.gene57208:intron | 35.0% | |
| CATGGAGATACCATTAAAGA+AGG | - | chr5.3:8758814-8758833 | MS.gene57208:CDS | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | - | chr5.3:8758755-8758774 | MS.gene57208:CDS | 35.0% | |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | + | chr5.3:8758723-8758742 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | - | chr5.3:8758720-8758739 | MS.gene57208:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | + | chr5.3:8758921-8758940 | None:intergenic | 35.0% |
| !!! | ACCTTTTAAAACCCTTTTCG+CGG | + | chr5.3:8759185-8759204 | None:intergenic | 35.0% |
| AACCATTCGTTACGAGCATT+AGG | + | chr5.3:8758997-8759016 | None:intergenic | 40.0% | |
| ACTTCTCTATTGACACTGTG+TGG | - | chr5.3:8759078-8759097 | MS.gene57208:intron | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr5.3:8759722-8759741 | None:intergenic | 40.0% | |
| ATTCGTTACGAGCATTAGGA+AGG | + | chr5.3:8758993-8759012 | None:intergenic | 40.0% | |
| ATTGACACTGTGTGGAGAAA+AGG | - | chr5.3:8759086-8759105 | MS.gene57208:intron | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr5.3:8759721-8759740 | None:intergenic | 40.0% | |
| TTCCTAATGCTCGTAACGAA+TGG | - | chr5.3:8758992-8759011 | MS.gene57208:CDS | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | + | chr5.3:8758975-8758994 | None:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | + | chr5.3:8759302-8759321 | None:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | + | chr5.3:8759303-8759322 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr5.3:8759552-8759571 | MS.gene57208:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr5.3:8759549-8759568 | MS.gene57208:CDS | 40.0% |
| !!! | ACCGCGAAAAGGGTTTTAAA+AGG | - | chr5.3:8759181-8759200 | MS.gene57208:intron | 40.0% |
| AATGGTATCTCCATGCCCTT+CGG | + | chr5.3:8758809-8758828 | None:intergenic | 45.0% | |
| CAGCAACAATACCGCGAAAA+GGG | - | chr5.3:8759171-8759190 | MS.gene57208:intron | 45.0% | |
| CATTTAAATGCCGAAGGGCA+TGG | - | chr5.3:8758796-8758815 | MS.gene57208:CDS | 45.0% | |
| GTAGCAACACATGACCACTA+TGG | - | chr5.3:8759312-8759331 | MS.gene57208:CDS | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr5.3:8759565-8759584 | MS.gene57208:CDS | 45.0% | |
| TATGGACAAACTCGTGGTCA+TGG | - | chr5.3:8759330-8759349 | MS.gene57208:CDS | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr5.3:8759558-8759577 | MS.gene57208:CDS | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | + | chr5.3:8759297-8759316 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | + | chr5.3:8758872-8758891 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr5.3:8759598-8759617 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | - | chr5.3:8759389-8759408 | MS.gene57208:CDS | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | - | chr5.3:8758879-8758898 | MS.gene57208:CDS | 50.0% | |
| GACCACGAGTTTGTCCATAG+TGG | + | chr5.3:8759329-8759348 | None:intergenic | 50.0% | |
| GACCACTATGGACAAACTCG+TGG | - | chr5.3:8759324-8759343 | MS.gene57208:CDS | 50.0% | |
| GCAGCAACAATACCGCGAAA+AGG | - | chr5.3:8759170-8759189 | MS.gene57208:intron | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | + | chr5.3:8758882-8758901 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | - | chr5.3:8759402-8759421 | MS.gene57208:CDS | 50.0% |
| CAAACTCGTGGTCATGGTCG+TGG | - | chr5.3:8759336-8759355 | MS.gene57208:CDS | 55.0% | |
| CGTGGTCGTGGTTATGGAAG+GGG | - | chr5.3:8759390-8759409 | MS.gene57208:CDS | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | - | chr5.3:8759388-8759407 | MS.gene57208:CDS | 55.0% | |
| AACCACACGACTCGTCCCAC+TGG | - | chr5.3:8759264-8759283 | MS.gene57208:CDS | 60.0% | |
| CATGGTCGTGGTCGTGGTCA+TGG | - | chr5.3:8759348-8759367 | MS.gene57208:CDS | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | - | chr5.3:8759384-8759403 | MS.gene57208:CDS | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | - | chr5.3:8759396-8759415 | MS.gene57208:CDS | 60.0% | |
| !! | GGGAATGGAGCACTGCCAGT+GGG | + | chr5.3:8759282-8759301 | None:intergenic | 60.0% |
| !! | TGGGAATGGAGCACTGCCAG+TGG | + | chr5.3:8759283-8759302 | None:intergenic | 60.0% |
| CATGGTCGTGGACGTGGACG+TGG | - | chr5.3:8759366-8759385 | MS.gene57208:CDS | 65.0% | |
| CGTGGTCATGGTCGTGGACG+TGG | - | chr5.3:8759360-8759379 | MS.gene57208:CDS | 65.0% | |
| CGTGGTCATGGTCGTGGTCG+TGG | - | chr5.3:8759342-8759361 | MS.gene57208:CDS | 65.0% | |
| CGTGGTCGTGGTCATGGTCG+TGG | - | chr5.3:8759354-8759373 | MS.gene57208:CDS | 65.0% | |
| TGCCAGTGGGACGAGTCGTG+TGG | + | chr5.3:8759269-8759288 | None:intergenic | 65.0% | |
| CGTGGACGTGGACGTGGACG+TGG | - | chr5.3:8759372-8759391 | MS.gene57208:CDS | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | - | chr5.3:8759378-8759397 | MS.gene57208:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 8758712 | 8759800 | 8758712 | ID=MS.gene57208 |
| chr5.3 | mRNA | 8758712 | 8759800 | 8758712 | ID=MS.gene57208.t1;Parent=MS.gene57208 |
| chr5.3 | exon | 8759207 | 8759800 | 8759207 | ID=MS.gene57208.t1.exon1;Parent=MS.gene57208.t1 |
| chr5.3 | CDS | 8759207 | 8759800 | 8759207 | ID=cds.MS.gene57208.t1;Parent=MS.gene57208.t1 |
| chr5.3 | exon | 8758712 | 8759014 | 8758712 | ID=MS.gene57208.t1.exon2;Parent=MS.gene57208.t1 |
| chr5.3 | CDS | 8758712 | 8759014 | 8758712 | ID=cds.MS.gene57208.t1;Parent=MS.gene57208.t1 |
| Gene Sequence |
| Protein sequence |
